Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57673.t1 | XP_013448012.1 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 2.30E-219 | 771.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57673.t1 | Q9LI89 | 48.5 | 388 | 184 | 5 | 4 | 375 | 34 | 421 | 1.0e-95 | 351.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57673.t1 | A0A072TX07 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 1.6e-219 | 771.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene57673 | 0.850756 | 1.38E-60 | -1.69E-46 |
| MS.gene049320 | MS.gene57673 | -0.804012 | 2.65E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene57673 | 0.814318 | 1.64E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene57673 | -0.820383 | 7.06E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene57673 | -0.822422 | 2.39E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene57673 | -0.820641 | 6.16E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene57673 | -0.806369 | 8.51E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene57673 | -0.822207 | 2.68E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene57673 | 0.835401 | 1.71E-56 | -1.69E-46 |
| MS.gene051127 | MS.gene57673 | 0.818513 | 1.88E-52 | -1.69E-46 |
| MS.gene051364 | MS.gene57673 | 0.837829 | 4.13E-57 | -1.69E-46 |
| MS.gene051366 | MS.gene57673 | 0.823976 | 1.04E-53 | -1.69E-46 |
| MS.gene051367 | MS.gene57673 | 0.801939 | 7.10E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene57673 | 0.824657 | 7.17E-54 | -1.69E-46 |
| MS.gene053549 | MS.gene57673 | 0.811612 | 6.42E-51 | -1.69E-46 |
| MS.gene053826 | MS.gene57673 | 0.808006 | 3.83E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene57673 | 0.800438 | 1.44E-48 | -1.69E-46 |
| MS.gene05470 | MS.gene57673 | -0.82376 | 1.16E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene57673 | -0.800136 | 1.66E-48 | -1.69E-46 |
| MS.gene054959 | MS.gene57673 | 0.838951 | 2.12E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene57673 | 0.819037 | 1.43E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene57673 | -0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene57673 | -0.8248 | 6.63E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene57673 | -0.800608 | 1.33E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene57673 | -0.800335 | 1.51E-48 | -1.69E-46 |
| MS.gene056218 | MS.gene57673 | 0.806583 | 7.67E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene57673 | -0.81795 | 2.53E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene57673 | -0.801635 | 8.20E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene57673 | 0.827982 | 1.16E-54 | -1.69E-46 |
| MS.gene056988 | MS.gene57673 | 0.809947 | 1.47E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene57673 | 0.820588 | 6.34E-53 | -1.69E-46 |
| MS.gene058068 | MS.gene57673 | 0.806988 | 6.30E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene57673 | 0.810963 | 8.88E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene57673 | 0.806098 | 9.70E-50 | -1.69E-46 |
| MS.gene058268 | MS.gene57673 | 0.808417 | 3.13E-50 | -1.69E-46 |
| MS.gene058269 | MS.gene57673 | 0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene058334 | MS.gene57673 | 0.801713 | 7.91E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene57673 | -0.804184 | 2.44E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene57673 | -0.81126 | 7.65E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene57673 | 0.814939 | 1.19E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene57673 | -0.815165 | 1.06E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene57673 | -0.801389 | 9.21E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene57673 | -0.811909 | 5.53E-51 | -1.69E-46 |
| MS.gene059756 | MS.gene57673 | -0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene059953 | MS.gene57673 | 0.825506 | 4.52E-54 | -1.69E-46 |
| MS.gene059955 | MS.gene57673 | 0.810472 | 1.13E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene57673 | 0.817284 | 3.57E-52 | -1.69E-46 |
| MS.gene060778 | MS.gene57673 | -0.807766 | 4.31E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene57673 | -0.840975 | 6.29E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57673.t1 | MTR_7g029290 | 97.619 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 0.0 | 763 |
| MS.gene57673.t1 | MTR_6g023805 | 64.433 | 388 | 116 | 6 | 1 | 376 | 28 | 405 | 3.08e-168 | 476 |
| MS.gene57673.t1 | MTR_2g094093 | 39.420 | 345 | 201 | 6 | 32 | 374 | 24 | 362 | 2.02e-77 | 243 |
| MS.gene57673.t1 | MTR_4g108280 | 36.388 | 371 | 213 | 10 | 18 | 374 | 17 | 378 | 2.85e-71 | 228 |
| MS.gene57673.t1 | MTR_0345s0010 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.07e-70 | 228 |
| MS.gene57673.t1 | MTR_0105s0020 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.48e-70 | 228 |
| MS.gene57673.t1 | MTR_0105s0130 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 3.56e-70 | 226 |
| MS.gene57673.t1 | MTR_0105s0170 | 37.736 | 371 | 212 | 11 | 13 | 374 | 86 | 446 | 2.10e-69 | 225 |
| MS.gene57673.t1 | MTR_0496s0010 | 38.095 | 378 | 195 | 13 | 13 | 374 | 55 | 409 | 2.41e-69 | 224 |
| MS.gene57673.t1 | MTR_0105s0040 | 37.736 | 371 | 212 | 11 | 13 | 374 | 171 | 531 | 5.73e-69 | 226 |
| MS.gene57673.t1 | MTR_4g022260 | 37.037 | 378 | 205 | 13 | 13 | 374 | 55 | 415 | 1.96e-68 | 222 |
| MS.gene57673.t1 | MTR_8g468030 | 39.244 | 344 | 192 | 9 | 39 | 374 | 161 | 495 | 3.55e-68 | 223 |
| MS.gene57673.t1 | MTR_1330s0010 | 38.136 | 354 | 202 | 10 | 13 | 357 | 86 | 431 | 8.47e-68 | 221 |
| MS.gene57673.t1 | MTR_4g022400 | 35.890 | 365 | 217 | 9 | 18 | 374 | 57 | 412 | 1.13e-67 | 219 |
| MS.gene57673.t1 | MTR_5g039390 | 37.435 | 382 | 218 | 11 | 5 | 374 | 102 | 474 | 1.64e-67 | 221 |
| MS.gene57673.t1 | MTR_8g085600 | 27.303 | 304 | 200 | 10 | 5 | 299 | 55 | 346 | 4.28e-24 | 103 |
| MS.gene57673.t1 | MTR_4g114110 | 25.168 | 298 | 198 | 10 | 9 | 298 | 69 | 349 | 3.80e-19 | 89.0 |
| MS.gene57673.t1 | MTR_3g073640 | 25.652 | 230 | 156 | 5 | 31 | 254 | 46 | 266 | 1.21e-16 | 81.6 |
| MS.gene57673.t1 | MTR_7g095220 | 25.957 | 235 | 145 | 8 | 32 | 248 | 8 | 231 | 2.45e-15 | 76.6 |
| MS.gene57673.t1 | MTR_8g080370 | 24.471 | 331 | 213 | 12 | 14 | 325 | 33 | 345 | 9.47e-15 | 75.5 |
| MS.gene57673.t1 | MTR_3g085570 | 24.272 | 309 | 201 | 11 | 32 | 325 | 39 | 329 | 1.54e-13 | 71.6 |
| MS.gene57673.t1 | MTR_2g019230 | 23.776 | 286 | 197 | 9 | 32 | 304 | 56 | 333 | 1.40e-12 | 68.9 |
| MS.gene57673.t1 | MTR_2g008440 | 25.543 | 368 | 199 | 14 | 31 | 326 | 46 | 410 | 1.67e-12 | 68.9 |
| MS.gene57673.t1 | MTR_2g012790 | 29.091 | 165 | 101 | 3 | 138 | 292 | 109 | 267 | 2.99e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57673.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57673.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57673.t1 | AT5G40680 | 47.757 | 379 | 192 | 4 | 4 | 376 | 37 | 415 | 1.98e-119 | 352 |
| MS.gene57673.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57673.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57673.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57673.t1 | AT1G14330 | 39.650 | 343 | 192 | 9 | 39 | 374 | 105 | 439 | 1.55e-69 | 225 |
| MS.gene57673.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57673.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57673.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57673.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57673.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57673.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57673.t1 | AT1G26930 | 36.340 | 377 | 206 | 10 | 13 | 374 | 62 | 419 | 5.25e-64 | 210 |
| MS.gene57673.t1 | AT1G26930 | 30.769 | 377 | 175 | 12 | 13 | 374 | 62 | 367 | 1.46e-41 | 150 |
| MS.gene57673.t1 | AT1G55270 | 26.449 | 276 | 185 | 8 | 32 | 299 | 82 | 347 | 1.00e-20 | 93.6 |
| MS.gene57673.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57673.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57673.t1 | AT4G35120 | 28.241 | 216 | 139 | 6 | 31 | 234 | 29 | 240 | 3.21e-13 | 70.9 |
| MS.gene57673.t1 | AT3G63220 | 24.510 | 306 | 186 | 13 | 41 | 328 | 17 | 295 | 3.44e-13 | 70.5 |
| MS.gene57673.t1 | AT1G60570 | 25.574 | 305 | 197 | 10 | 10 | 299 | 2 | 291 | 2.22e-12 | 68.2 |
| MS.gene57673.t1 | AT1G55270 | 25.500 | 200 | 134 | 7 | 105 | 299 | 32 | 221 | 1.84e-11 | 65.1 |
| MS.gene57673.t1 | AT4G29370 | 29.500 | 200 | 118 | 8 | 17 | 204 | 14 | 202 | 2.36e-11 | 65.1 |
| MS.gene57673.t1 | AT1G22040 | 25.751 | 233 | 151 | 7 | 31 | 254 | 46 | 265 | 3.38e-11 | 65.1 |
| MS.gene57673.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57673.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57673.t1 | AT2G29830 | 21.446 | 401 | 270 | 15 | 1 | 375 | 1 | 382 | 4.49e-11 | 64.3 |
Find 75 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCTATAGCGAAATTT+TGG | 0.201938 | 7.4:+72224853 | MS.gene57673:CDS |
| AATTTGACGGACCTGTTATT+TGG | 0.224264 | 7.4:+72224714 | MS.gene57673:CDS |
| TCAGATTACAGCTTTGAATT+TGG | 0.229608 | 7.4:+72224641 | MS.gene57673:CDS |
| GGGTCAGAGTTATTTGTTTC+GGG | 0.243484 | 7.4:+72224686 | MS.gene57673:CDS |
| GACAAGAGATTCTATGTTAT+TGG | 0.250445 | 7.4:+72224968 | MS.gene57673:CDS |
| AGTTTCCAATGTTGCGATCT+TGG | 0.284283 | 7.4:-72224446 | None:intergenic |
| CTATTTGCTTCAGCTAGTTC+TGG | 0.294486 | 7.4:+72224797 | MS.gene57673:CDS |
| GCGGCGAGATCTACAAAATA+AGG | 0.302206 | 7.4:+72224507 | MS.gene57673:CDS |
| TTTCTCTTTGAATTCATATT+CGG | 0.325901 | 7.4:-72224923 | None:intergenic |
| AGCTGATGCACAAGGCGGTT+GGG | 0.354563 | 7.4:+72225228 | MS.gene57673:CDS |
| CAGAACTAGCTGAAGCAAAT+AGG | 0.366562 | 7.4:-72224796 | None:intergenic |
| GTTGAAAGACATTCCACTTT+CGG | 0.373291 | 7.4:+72225075 | MS.gene57673:CDS |
| TACAGCTTAATCATTTCATT+AGG | 0.374102 | 7.4:+72225398 | MS.gene57673:CDS |
| GGTGATGAGTTACTTGTTAT+CGG | 0.384004 | 7.4:+72225271 | MS.gene57673:CDS |
| CATTTCAATTTCTCACCCTC+AGG | 0.390524 | 7.4:-72225352 | None:intergenic |
| AGGGTCAGAGTTATTTGTTT+CGG | 0.390691 | 7.4:+72224685 | MS.gene57673:CDS |
| CTGTAATCTGATTGAATCCT+TGG | 0.399975 | 7.4:-72224629 | None:intergenic |
| GAATCATATCGCCAAATAAC+AGG | 0.403217 | 7.4:-72224725 | None:intergenic |
| ATTTGAAAGGGATCAATAAC+TGG | 0.407898 | 7.4:+72225179 | MS.gene57673:CDS |
| TGTTATTTGGCGATATGATT+CGG | 0.418939 | 7.4:+72224727 | MS.gene57673:CDS |
| AATTTCTCACCCTCAGGATC+AGG | 0.424534 | 7.4:-72225346 | None:intergenic |
| CGAAAGCAATTGGTGGGGTA+TGG | 0.427240 | 7.4:+72224577 | MS.gene57673:CDS |
| TACAAAATAAGGAAAGAGTT+AGG | 0.428564 | 7.4:+72224518 | MS.gene57673:CDS |
| TTCGCATAAAGAACGAGCTT+TGG | 0.431074 | 7.4:+72225306 | MS.gene57673:CDS |
| GGAATGTCTTTCAACATGTC+AGG | 0.452600 | 7.4:-72225067 | None:intergenic |
| TTTGAATTCATATTCGGTAA+AGG | 0.467260 | 7.4:-72224917 | None:intergenic |
| GCTGATGCACAAGGCGGTTG+GGG | 0.472323 | 7.4:+72225229 | MS.gene57673:CDS |
| CTGAGGGTGAGAAATTGAAA+TGG | 0.473233 | 7.4:+72225353 | MS.gene57673:CDS |
| TAGCTAGCGGCGAAAGCAAT+TGG | 0.476177 | 7.4:+72224567 | MS.gene57673:CDS |
| AGCTTCCTGCTTGATTTGAA+AGG | 0.476416 | 7.4:-72224605 | None:intergenic |
| CGAGCTAGAGACATCGATCT+TGG | 0.476661 | 7.4:+72224415 | MS.gene57673:CDS |
| ATTCTATGTTATTGGTGGAC+AGG | 0.479496 | 7.4:+72224976 | MS.gene57673:CDS |
| AGCTGAAAGTGTATTTGAAA+GGG | 0.490575 | 7.4:+72225167 | MS.gene57673:CDS |
| AATCAAGCAGGAAGCTTCCA+AGG | 0.494763 | 7.4:+72224612 | MS.gene57673:CDS |
| GAGCTGAAAGTGTATTTGAA+AGG | 0.497856 | 7.4:+72225166 | MS.gene57673:CDS |
| TAGGAATTGTGCTGTGATGA+TGG | 0.506085 | 7.4:+72225417 | MS.gene57673:CDS |
| GAGCTGATGCACAAGGCGGT+TGG | 0.506566 | 7.4:+72225227 | MS.gene57673:CDS |
| GATTTCCAAGATCGCAACAT+TGG | 0.509774 | 7.4:+72224441 | MS.gene57673:CDS |
| CCTTGTGCATCAGCTCTCAC+AGG | 0.510167 | 7.4:-72225220 | None:intergenic |
| AAATGGAAGCAAATTGATTG+TGG | 0.518481 | 7.4:+72225370 | MS.gene57673:CDS |
| GAAAGAAGAATCTTTACCTC+AGG | 0.525784 | 7.4:+72224364 | MS.gene57673:CDS |
| GTTTCGGGTAAAGAATTTGA+CGG | 0.527787 | 7.4:+72224701 | MS.gene57673:CDS |
| GGTGTCCAAAATTTCGCTAT+AGG | 0.532541 | 7.4:-72224858 | None:intergenic |
| TACTTGTTGTCCTGATCCTG+AGG | 0.533715 | 7.4:+72225336 | MS.gene57673:CDS |
| AAGAGATTCTATGTTATTGG+TGG | 0.534243 | 7.4:+72224971 | MS.gene57673:CDS |
| GATACTAATGAATGGTTCAA+AGG | 0.541527 | 7.4:+72224749 | MS.gene57673:CDS |
| ATGATTCGGATACTAATGAA+TGG | 0.555866 | 7.4:+72224741 | MS.gene57673:CDS |
| TGAAGAACCGTTGCCATCAG+AGG | 0.562265 | 7.4:-72224339 | None:intergenic |
| TCGACGAAGAAACAAACACT+TGG | 0.563005 | 7.4:+72225035 | MS.gene57673:CDS |
| GTCCGTTCATGATTAATCCT+AGG | 0.572154 | 7.4:+72224771 | MS.gene57673:CDS |
| GCAATTGGTGGGGTATGGAA+TGG | 0.578887 | 7.4:+72224582 | MS.gene57673:CDS |
| TAGCGGCGAAAGCAATTGGT+GGG | 0.579866 | 7.4:+72224571 | MS.gene57673:CDS |
| TTACCACCCTCTGATGGCAA+CGG | 0.580464 | 7.4:+72224332 | MS.gene57673:CDS |
| CGTTCTTTATGCGAAACAGA+AGG | 0.588464 | 7.4:-72225298 | None:intergenic |
| GAAGAACCGTTGCCATCAGA+GGG | 0.591725 | 7.4:-72224338 | None:intergenic |
| TATTAACAACAGCTATCAGA+GGG | 0.594157 | 7.4:-72225111 | None:intergenic |
| GGAGAGTGCGAATCCGAAAG+TGG | 0.596088 | 7.4:-72225088 | None:intergenic |
| ACTTGTTGTCCTGATCCTGA+GGG | 0.606724 | 7.4:+72225337 | MS.gene57673:CDS |
| GGTGTTGCATTTAAATCACT+TGG | 0.608539 | 7.4:+72225250 | MS.gene57673:CDS |
| GACTTGTTACCACCCTCTGA+TGG | 0.609485 | 7.4:+72224326 | MS.gene57673:CDS |
| CACCTAGGATTAATCATGAA+CGG | 0.612535 | 7.4:-72224773 | None:intergenic |
| AATGGCCTTTCAAATCAAGC+AGG | 0.615937 | 7.4:+72224600 | MS.gene57673:CDS |
| AGGAATTGTGCTGTGATGAT+GGG | 0.616810 | 7.4:+72225418 | MS.gene57673:CDS |
| CTAGCGGCGAAAGCAATTGG+TGG | 0.628757 | 7.4:+72224570 | MS.gene57673:CDS |
| TTATTAACAACAGCTATCAG+AGG | 0.633695 | 7.4:-72225112 | None:intergenic |
| CCTGTGAGAGCTGATGCACA+AGG | 0.635244 | 7.4:+72225220 | MS.gene57673:CDS |
| GCTGAAGCAAATAGGCACCT+AGG | 0.638194 | 7.4:-72224788 | None:intergenic |
| AGCGGCGAAAGCAATTGGTG+GGG | 0.663533 | 7.4:+72224572 | MS.gene57673:CDS |
| AGATTATAATCTGCATCCTG+AGG | 0.665618 | 7.4:-72224380 | None:intergenic |
| GAACCGTTGCCATCAGAGGG+TGG | 0.673262 | 7.4:-72224335 | None:intergenic |
| ATTAACAACAGCTATCAGAG+GGG | 0.676676 | 7.4:-72225110 | None:intergenic |
| TGGACAGGACGAGAATCGAA+AGG | 0.677851 | 7.4:+72224991 | MS.gene57673:CDS |
| TTTCGAATCATATTTCTCAG+CGG | 0.689567 | 7.4:-72224879 | None:intergenic |
| TTAACAACAGCTATCAGAGG+GGG | 0.692839 | 7.4:-72225109 | None:intergenic |
| GTGAGAGCTGATGCACAAGG+CGG | 0.771409 | 7.4:+72225223 | MS.gene57673:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATAACTGGAAAAAATT+AGG | + | chr7.4:72225190-72225209 | MS.gene57673:CDS | 20.0% |
| !! | TTTCTCTTTGAATTCATATT+CGG | - | chr7.4:72224926-72224945 | None:intergenic | 20.0% |
| ! | ATGATTCGAAAACAAAAACT+TGG | + | chr7.4:72224891-72224910 | MS.gene57673:CDS | 25.0% |
| ! | TACAAAATAAGGAAAGAGTT+AGG | + | chr7.4:72224518-72224537 | MS.gene57673:CDS | 25.0% |
| ! | TACAGCTTAATCATTTCATT+AGG | + | chr7.4:72225398-72225417 | MS.gene57673:CDS | 25.0% |
| ! | TTTGAATTCATATTCGGTAA+AGG | - | chr7.4:72224920-72224939 | None:intergenic | 25.0% |
| !! | ATAAAGAATCATTTTCAGCA+GGG | + | chr7.4:72224666-72224685 | MS.gene57673:CDS | 25.0% |
| !! | TCAAAGAGAAAATTTTGTTC+AGG | + | chr7.4:72224935-72224954 | MS.gene57673:CDS | 25.0% |
| AAATGGAAGCAAATTGATTG+TGG | + | chr7.4:72225370-72225389 | MS.gene57673:CDS | 30.0% | |
| AAGAGATTCTATGTTATTGG+TGG | + | chr7.4:72224971-72224990 | MS.gene57673:CDS | 30.0% | |
| ATTTGAAAGGGATCAATAAC+TGG | + | chr7.4:72225179-72225198 | MS.gene57673:CDS | 30.0% | |
| GACAAGAGATTCTATGTTAT+TGG | + | chr7.4:72224968-72224987 | MS.gene57673:CDS | 30.0% | |
| GATACTAATGAATGGTTCAA+AGG | + | chr7.4:72224749-72224768 | MS.gene57673:CDS | 30.0% | |
| TATTAACAACAGCTATCAGA+GGG | - | chr7.4:72225114-72225133 | None:intergenic | 30.0% | |
| TTATTAACAACAGCTATCAG+AGG | - | chr7.4:72225115-72225134 | None:intergenic | 30.0% | |
| TTTCGAATCATATTTCTCAG+CGG | - | chr7.4:72224882-72224901 | None:intergenic | 30.0% | |
| ! | AAACACCTATAGCGAAATTT+TGG | + | chr7.4:72224853-72224872 | MS.gene57673:CDS | 30.0% |
| ! | AGCTGAAAGTGTATTTGAAA+GGG | + | chr7.4:72225167-72225186 | MS.gene57673:CDS | 30.0% |
| ! | ATGATTCGGATACTAATGAA+TGG | + | chr7.4:72224741-72224760 | MS.gene57673:CDS | 30.0% |
| ! | ATTTTGTTCAGGATGTTACA+TGG | + | chr7.4:72224946-72224965 | MS.gene57673:CDS | 30.0% |
| ! | GATAAAGAATCATTTTCAGC+AGG | + | chr7.4:72224665-72224684 | MS.gene57673:CDS | 30.0% |
| ! | TGTTATTTGGCGATATGATT+CGG | + | chr7.4:72224727-72224746 | MS.gene57673:CDS | 30.0% |
| ! | TTTTTAACCCTGATGAAAAG+CGG | + | chr7.4:72224488-72224507 | MS.gene57673:CDS | 30.0% |
| !! | TCAGATTACAGCTTTGAATT+TGG | + | chr7.4:72224641-72224660 | MS.gene57673:CDS | 30.0% |
| AGATTATAATCTGCATCCTG+AGG | - | chr7.4:72224383-72224402 | None:intergenic | 35.0% | |
| AGGGTCAGAGTTATTTGTTT+CGG | + | chr7.4:72224685-72224704 | MS.gene57673:CDS | 35.0% | |
| ATTAACAACAGCTATCAGAG+GGG | - | chr7.4:72225113-72225132 | None:intergenic | 35.0% | |
| CACCTAGGATTAATCATGAA+CGG | - | chr7.4:72224776-72224795 | None:intergenic | 35.0% | |
| CTAGCTAACATAAAAACGGA+CGG | - | chr7.4:72224554-72224573 | None:intergenic | 35.0% | |
| CTGTAATCTGATTGAATCCT+TGG | - | chr7.4:72224632-72224651 | None:intergenic | 35.0% | |
| GAAAGAAGAATCTTTACCTC+AGG | + | chr7.4:72224364-72224383 | MS.gene57673:CDS | 35.0% | |
| GAATCATATCGCCAAATAAC+AGG | - | chr7.4:72224728-72224747 | None:intergenic | 35.0% | |
| GTTGAAAGACATTCCACTTT+CGG | + | chr7.4:72225075-72225094 | MS.gene57673:CDS | 35.0% | |
| ! | AATTTGACGGACCTGTTATT+TGG | + | chr7.4:72224714-72224733 | MS.gene57673:CDS | 35.0% |
| ! | ATTCTATGTTATTGGTGGAC+AGG | + | chr7.4:72224976-72224995 | MS.gene57673:CDS | 35.0% |
| ! | GAGCTGAAAGTGTATTTGAA+AGG | + | chr7.4:72225166-72225185 | MS.gene57673:CDS | 35.0% |
| ! | GGTGTTGCATTTAAATCACT+TGG | + | chr7.4:72225250-72225269 | MS.gene57673:CDS | 35.0% |
| ! | GTTTCGGGTAAAGAATTTGA+CGG | + | chr7.4:72224701-72224720 | MS.gene57673:CDS | 35.0% |
| !! | AATTTTGCTTTTGTTGCAGG+TGG | + | chr7.4:72224821-72224840 | MS.gene57673:CDS | 35.0% |
| !! | GGTAATTTTGCTTTTGTTGC+AGG | + | chr7.4:72224818-72224837 | MS.gene57673:CDS | 35.0% |
| !! | GGTGATGAGTTACTTGTTAT+CGG | + | chr7.4:72225271-72225290 | MS.gene57673:CDS | 35.0% |
| !! | TCCGTTTTTATGTTAGCTAG+CGG | + | chr7.4:72224554-72224573 | MS.gene57673:CDS | 35.0% |
| AATGGCCTTTCAAATCAAGC+AGG | + | chr7.4:72224600-72224619 | MS.gene57673:CDS | 40.0% | |
| AGGAATTGTGCTGTGATGAT+GGG | + | chr7.4:72225418-72225437 | MS.gene57673:CDS | 40.0% | |
| AGTTTCCAATGTTGCGATCT+TGG | - | chr7.4:72224449-72224468 | None:intergenic | 40.0% | |
| CAGAACTAGCTGAAGCAAAT+AGG | - | chr7.4:72224799-72224818 | None:intergenic | 40.0% | |
| CATTTCAATTTCTCACCCTC+AGG | - | chr7.4:72225355-72225374 | None:intergenic | 40.0% | |
| CGTTCTTTATGCGAAACAGA+AGG | - | chr7.4:72225301-72225320 | None:intergenic | 40.0% | |
| CTATTTGCTTCAGCTAGTTC+TGG | + | chr7.4:72224797-72224816 | MS.gene57673:CDS | 40.0% | |
| CTGAGGGTGAGAAATTGAAA+TGG | + | chr7.4:72225353-72225372 | MS.gene57673:CDS | 40.0% | |
| GATTTCCAAGATCGCAACAT+TGG | + | chr7.4:72224441-72224460 | MS.gene57673:CDS | 40.0% | |
| GCCGCTAGCTAACATAAAAA+CGG | - | chr7.4:72224558-72224577 | None:intergenic | 40.0% | |
| GGAATGTCTTTCAACATGTC+AGG | - | chr7.4:72225070-72225089 | None:intergenic | 40.0% | |
| GGGTCAGAGTTATTTGTTTC+GGG | + | chr7.4:72224686-72224705 | MS.gene57673:CDS | 40.0% | |
| GGTGTCCAAAATTTCGCTAT+AGG | - | chr7.4:72224861-72224880 | None:intergenic | 40.0% | |
| GTCCGTTCATGATTAATCCT+AGG | + | chr7.4:72224771-72224790 | MS.gene57673:CDS | 40.0% | |
| TAGGAATTGTGCTGTGATGA+TGG | + | chr7.4:72225417-72225436 | MS.gene57673:CDS | 40.0% | |
| TCGACGAAGAAACAAACACT+TGG | + | chr7.4:72225035-72225054 | MS.gene57673:CDS | 40.0% | |
| TTAACAACAGCTATCAGAGG+GGG | - | chr7.4:72225112-72225131 | None:intergenic | 40.0% | |
| TTCGCATAAAGAACGAGCTT+TGG | + | chr7.4:72225306-72225325 | MS.gene57673:CDS | 40.0% | |
| ! | AGCTTCCTGCTTGATTTGAA+AGG | - | chr7.4:72224608-72224627 | None:intergenic | 40.0% |
| AATCAAGCAGGAAGCTTCCA+AGG | + | chr7.4:72224612-72224631 | MS.gene57673:CDS | 45.0% | |
| AATTTCTCACCCTCAGGATC+AGG | - | chr7.4:72225349-72225368 | None:intergenic | 45.0% | |
| GCGGCGAGATCTACAAAATA+AGG | + | chr7.4:72224507-72224526 | MS.gene57673:CDS | 45.0% | |
| ! | TGCTTTTGTTGCAGGTGGTT+TGG | + | chr7.4:72224826-72224845 | MS.gene57673:CDS | 45.0% |
| !! | ACTTGTTGTCCTGATCCTGA+GGG | + | chr7.4:72225337-72225356 | MS.gene57673:CDS | 45.0% |
| !! | TACTTGTTGTCCTGATCCTG+AGG | + | chr7.4:72225336-72225355 | MS.gene57673:CDS | 45.0% |
| CGAAAGCAATTGGTGGGGTA+TGG | + | chr7.4:72224577-72224596 | MS.gene57673:CDS | 50.0% | |
| CGAGCTAGAGACATCGATCT+TGG | + | chr7.4:72224415-72224434 | MS.gene57673:CDS | 50.0% | |
| GAAGAACCGTTGCCATCAGA+GGG | - | chr7.4:72224341-72224360 | None:intergenic | 50.0% | |
| GCAATTGGTGGGGTATGGAA+TGG | + | chr7.4:72224582-72224601 | MS.gene57673:CDS | 50.0% | |
| GCTGAAGCAAATAGGCACCT+AGG | - | chr7.4:72224791-72224810 | None:intergenic | 50.0% | |
| TAGCGGCGAAAGCAATTGGT+GGG | + | chr7.4:72224571-72224590 | MS.gene57673:CDS | 50.0% | |
| TAGCTAGCGGCGAAAGCAAT+TGG | + | chr7.4:72224567-72224586 | MS.gene57673:CDS | 50.0% | |
| TGAAGAACCGTTGCCATCAG+AGG | - | chr7.4:72224342-72224361 | None:intergenic | 50.0% | |
| TGGACAGGACGAGAATCGAA+AGG | + | chr7.4:72224991-72225010 | MS.gene57673:CDS | 50.0% | |
| TTACCACCCTCTGATGGCAA+CGG | + | chr7.4:72224332-72224351 | MS.gene57673:CDS | 50.0% | |
| !! | GACTTGTTACCACCCTCTGA+TGG | + | chr7.4:72224326-72224345 | MS.gene57673:CDS | 50.0% |
| !!! | AGATCTCGCCGCTTTTCATC+AGG | - | chr7.4:72224499-72224518 | None:intergenic | 50.0% |
| !!! | GATCTCGCCGCTTTTCATCA+GGG | - | chr7.4:72224498-72224517 | None:intergenic | 50.0% |
| AGCGGCGAAAGCAATTGGTG+GGG | + | chr7.4:72224572-72224591 | MS.gene57673:CDS | 55.0% | |
| AGCTGATGCACAAGGCGGTT+GGG | + | chr7.4:72225228-72225247 | MS.gene57673:CDS | 55.0% | |
| CCTGTGAGAGCTGATGCACA+AGG | + | chr7.4:72225220-72225239 | MS.gene57673:CDS | 55.0% | |
| CCTTGTGCATCAGCTCTCAC+AGG | - | chr7.4:72225223-72225242 | None:intergenic | 55.0% | |
| CTAGCGGCGAAAGCAATTGG+TGG | + | chr7.4:72224570-72224589 | MS.gene57673:CDS | 55.0% | |
| GTGAGAGCTGATGCACAAGG+CGG | + | chr7.4:72225223-72225242 | MS.gene57673:CDS | 55.0% | |
| ! | GGAGAGTGCGAATCCGAAAG+TGG | - | chr7.4:72225091-72225110 | None:intergenic | 55.0% |
| GAACCGTTGCCATCAGAGGG+TGG | - | chr7.4:72224338-72224357 | None:intergenic | 60.0% | |
| GAGCTGATGCACAAGGCGGT+TGG | + | chr7.4:72225227-72225246 | MS.gene57673:CDS | 60.0% | |
| GCTGATGCACAAGGCGGTTG+GGG | + | chr7.4:72225229-72225248 | MS.gene57673:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72224314 | 72225450 | 72224314 | ID=MS.gene57673 |
| chr7.4 | mRNA | 72224314 | 72225450 | 72224314 | ID=MS.gene57673.t1;Parent=MS.gene57673 |
| chr7.4 | exon | 72224314 | 72225450 | 72224314 | ID=MS.gene57673.t1.exon1;Parent=MS.gene57673.t1 |
| chr7.4 | CDS | 72224314 | 72225450 | 72224314 | ID=cds.MS.gene57673.t1;Parent=MS.gene57673.t1 |
| Gene Sequence |
| Protein sequence |
