AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59166

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59166.t1 MTR_1g040195 92.810 459 10 1 1 459 1 436 0.0 868
MS.gene59166.t1 MTR_1g040215 76.356 461 107 2 1 459 1 461 0.0 734
MS.gene59166.t1 MTR_1g040175 74.620 461 112 3 1 459 1 458 0.0 701
MS.gene59166.t1 MTR_1g040315 73.913 460 76 4 1 459 1 417 0.0 675
MS.gene59166.t1 MTR_1g040320 69.565 460 104 2 1 459 1 425 0.0 644
MS.gene59166.t1 MTR_5g076700 58.913 460 179 6 5 459 6 460 0.0 560
MS.gene59166.t1 MTR_7g114560 58.696 460 179 7 5 459 6 459 0.0 559
MS.gene59166.t1 MTR_7g114470 58.478 460 182 5 5 459 7 462 0.0 555
MS.gene59166.t1 MTR_7g114550 57.837 453 176 7 2 446 3 448 0.0 552
MS.gene59166.t1 MTR_7g114520 56.745 467 179 8 5 459 7 462 0.0 544
MS.gene59166.t1 MTR_1g081660 60.502 438 168 5 4 438 13 448 0.0 532
MS.gene59166.t1 MTR_7g114490 58.237 431 172 5 5 430 6 433 0.0 528
MS.gene59166.t1 MTR_0042s0190 69.360 297 89 2 165 459 1 297 4.11e-150 429
MS.gene59166.t1 MTR_7g114510 55.000 320 130 7 147 459 8 320 1.27e-116 345
MS.gene59166.t1 MTR_0042s0190 69.828 232 68 2 230 459 1 232 8.27e-115 337
MS.gene59166.t1 MTR_0502s0010 61.157 242 84 5 100 335 1 238 2.09e-99 298
MS.gene59166.t1 MTR_7g114420 52.000 300 107 7 165 459 1 268 1.49e-97 294
MS.gene59166.t1 MTR_3g035000 60.444 225 62 4 195 417 39 238 1.11e-81 255
MS.gene59166.t1 MTR_8g039950 92.248 129 10 0 1 129 1 129 2.84e-81 247
MS.gene59166.t1 MTR_0042s0230 77.551 147 33 0 1 147 1 147 1.10e-78 241
MS.gene59166.t1 MTR_3g098980 32.944 428 268 9 9 430 14 428 1.70e-77 248
MS.gene59166.t1 MTR_8g096530 32.517 449 285 9 1 444 1 436 8.05e-77 247
MS.gene59166.t1 MTR_7g027175 33.854 384 242 7 12 389 19 396 1.86e-72 235
MS.gene59166.t1 MTR_7g114500 57.823 147 62 0 5 151 6 152 1.83e-60 194
MS.gene59166.t1 MTR_1g053315 31.603 443 277 15 17 438 26 463 3.44e-57 196
MS.gene59166.t1 MTR_4g055170 29.139 453 296 9 3 435 6 453 9.04e-56 192
MS.gene59166.t1 MTR_3g112180 32.908 392 239 10 19 397 22 402 3.75e-55 190
MS.gene59166.t1 MTR_3g051790 56.849 146 63 0 2 147 6 151 9.74e-54 178
MS.gene59166.t1 MTR_4g007540 30.787 432 267 16 16 430 10 426 4.83e-50 176
MS.gene59166.t1 MTR_3g071320 30.254 433 274 11 17 434 13 432 7.22e-49 173
MS.gene59166.t1 MTR_2g105330 29.040 427 278 11 17 430 11 425 1.67e-47 169
MS.gene59166.t1 MTR_8g075610 29.318 440 264 11 17 434 2 416 2.25e-46 166
MS.gene59166.t1 MTR_2g064495 28.806 427 279 11 17 430 11 425 3.33e-46 166
MS.gene59166.t1 MTR_7g110610 28.148 405 227 12 5 401 1 349 1.26e-42 155
MS.gene59166.t1 MTR_7g085930 28.632 475 263 18 9 432 6 455 3.74e-42 155
MS.gene59166.t1 MTR_7g084940 28.211 436 292 10 5 430 12 436 5.18e-42 155
MS.gene59166.t1 MTR_7g085990 27.719 469 275 17 9 432 2 451 2.29e-40 150
MS.gene59166.t1 MTR_4g127650 27.617 449 280 14 17 434 18 452 2.56e-39 147
MS.gene59166.t1 MTR_6g053810 28.851 409 255 13 56 437 63 462 6.51e-39 147
MS.gene59166.t1 MTR_2g015430 28.675 415 281 12 30 434 25 434 1.48e-38 145
MS.gene59166.t1 MTR_7g084910 27.723 404 247 12 1 389 11 384 2.52e-38 144
MS.gene59166.t1 MTR_4g097090 28.541 466 287 17 1 434 1 452 6.54e-37 141
MS.gene59166.t1 MTR_3g026950 26.277 411 263 10 13 390 6 409 1.07e-36 140
MS.gene59166.t1 MTR_3g465990 27.473 455 283 16 16 437 17 457 2.10e-36 140
MS.gene59166.t1 MTR_3g465980 27.692 455 282 15 16 437 17 457 1.03e-35 138
MS.gene59166.t1 MTR_7g086000 27.318 399 239 17 61 422 57 441 1.16e-35 137
MS.gene59166.t1 MTR_3g465930 27.212 452 292 14 16 437 17 461 6.63e-34 133
MS.gene59166.t1 MTR_7g056087 26.577 444 276 14 17 432 12 433 1.58e-33 131
MS.gene59166.t1 MTR_4g013050 24.374 439 269 13 12 430 7 402 1.45e-30 122
MS.gene59166.t1 MTR_3g465910 27.293 458 281 16 16 437 17 458 3.28e-30 123
MS.gene59166.t1 MTR_3g465880 27.311 476 264 17 16 437 17 464 1.13e-29 121
MS.gene59166.t1 MTR_4g088765 67.500 80 24 1 1 78 1 80 3.67e-29 110
MS.gene59166.t1 MTR_3g049150 25.750 400 263 11 17 388 10 403 6.22e-29 119
MS.gene59166.t1 MTR_3g101930 28.182 440 251 19 6 399 4 424 2.60e-27 114
MS.gene59166.t1 MTR_3g101960 29.781 319 199 12 23 327 30 337 1.68e-26 111
MS.gene59166.t1 MTR_3g465950 27.566 341 219 8 16 336 17 349 2.83e-26 111
MS.gene59166.t1 MTR_4g094835 25.616 406 258 15 46 428 49 433 8.17e-26 109
MS.gene59166.t1 MTR_0190s0020 27.002 437 251 19 23 428 30 429 3.37e-25 107
MS.gene59166.t1 MTR_3g101900 26.818 440 243 18 9 399 7 416 3.40e-25 107
MS.gene59166.t1 MTR_4g094838 24.884 430 260 14 23 428 29 419 4.47e-25 107
MS.gene59166.t1 MTR_5g090830 25.383 457 274 16 7 425 8 435 4.99e-25 107
MS.gene59166.t1 MTR_3g086020 23.937 447 286 17 5 435 1 409 6.71e-25 106
MS.gene59166.t1 MTR_5g090810 24.893 466 265 16 7 425 8 435 1.41e-24 106
MS.gene59166.t1 MTR_4g022290 26.515 396 243 13 23 395 31 401 2.12e-24 105
MS.gene59166.t1 MTR_4g094842 26.328 433 267 17 23 428 28 435 2.30e-24 105
MS.gene59166.t1 MTR_4g022800 26.606 436 259 20 23 428 25 429 2.53e-24 105
MS.gene59166.t1 MTR_5g084870 23.980 392 255 14 15 386 9 377 2.98e-24 105
MS.gene59166.t1 MTR_5g090880 26.000 400 235 15 61 425 64 437 4.65e-24 104
MS.gene59166.t1 MTR_5g084850 24.682 393 250 14 15 386 9 376 7.17e-24 103
MS.gene59166.t1 MTR_3g102020 26.517 445 274 19 24 441 31 449 8.25e-24 103
MS.gene59166.t1 MTR_4g094828 24.661 442 261 17 24 428 31 437 9.14e-24 103
MS.gene59166.t1 MTR_8g041770 24.886 438 275 15 14 428 22 428 2.11e-23 102
MS.gene59166.t1 MTR_4g094832 27.809 356 213 14 94 428 85 417 2.21e-23 102
MS.gene59166.t1 MTR_7g026860 27.103 428 263 18 23 428 25 425 5.08e-23 101
MS.gene59166.t1 MTR_3g088420 26.744 430 271 17 23 435 25 427 6.04e-23 101
MS.gene59166.t1 MTR_8g079950 23.983 467 285 18 15 448 9 438 3.73e-22 99.0
MS.gene59166.t1 MTR_3g450710 25.935 401 223 15 64 423 65 432 5.03e-22 98.6
MS.gene59166.t1 MTR_8g079710 26.757 441 250 21 23 428 30 432 5.13e-22 98.6
MS.gene59166.t1 MTR_3g101970 27.000 400 245 16 23 399 30 405 6.12e-22 98.2
MS.gene59166.t1 MTR_3g450690 24.752 404 221 14 64 423 72 436 6.68e-22 98.6
MS.gene59166.t1 MTR_3g070170 25.128 390 243 12 53 399 55 438 7.84e-22 98.2
MS.gene59166.t1 MTR_3g102010 27.208 419 231 17 24 399 32 419 8.30e-22 98.2
MS.gene59166.t1 MTR_4g022720 25.000 424 274 14 23 428 25 422 2.53e-21 96.3
MS.gene59166.t1 MTR_4g022670 25.116 430 272 18 23 428 25 428 2.90e-21 96.3
MS.gene59166.t1 MTR_8g079970 27.673 318 179 15 15 309 9 298 1.72e-20 94.0
MS.gene59166.t1 MTR_3g465870 28.378 296 188 9 16 294 19 307 2.15e-20 93.6
MS.gene59166.t1 MTR_4g022760 27.707 314 198 9 23 321 25 324 4.89e-19 89.4
MS.gene59166.t1 MTR_4g022825 25.450 444 252 19 23 428 25 427 6.53e-19 89.4
MS.gene59166.t1 MTR_8g079980 25.649 308 199 12 15 309 9 299 2.08e-18 87.8
MS.gene59166.t1 MTR_2g007880 24.412 340 212 14 17 340 25 335 9.59e-18 85.9
MS.gene59166.t1 MTR_5g084900 22.727 330 210 12 64 386 9 300 2.74e-17 83.6
MS.gene59166.t1 MTR_7g114417 61.538 52 19 1 89 139 29 80 1.40e-15 72.0
MS.gene59166.t1 MTR_3g051820 48.958 96 43 4 365 459 1 91 6.64e-14 67.8
MS.gene59166.t1 MTR_6g016815 28.774 212 122 9 59 249 64 267 4.78e-12 67.8
MS.gene59166.t1 MTR_3g102030 25.325 308 185 12 90 385 42 316 8.10e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59166.t1 AT5G17540 57.940 466 184 9 1 459 1 461 0.0 526
MS.gene59166.t1 AT3G03480 57.336 443 174 11 5 439 15 450 6.53e-170 485
MS.gene59166.t1 AT5G41040 31.891 439 279 9 4 434 28 454 4.49e-79 253
MS.gene59166.t1 AT5G41040 31.891 439 279 9 4 434 12 438 5.00e-79 252
MS.gene59166.t1 AT5G63560 33.183 443 270 11 1 435 1 425 1.35e-77 248
MS.gene59166.t1 AT3G48720 31.121 437 281 11 1 430 1 424 2.90e-72 234
MS.gene59166.t1 AT1G27620 29.754 447 283 12 10 444 10 437 2.21e-55 191
MS.gene59166.t1 AT3G62160 29.540 457 276 16 5 444 1 428 2.78e-52 182
MS.gene59166.t1 AT1G65450 29.605 456 285 13 1 438 12 449 7.64e-51 179
MS.gene59166.t1 AT2G40230 32.300 387 238 11 17 388 13 390 1.06e-47 170
MS.gene59166.t1 AT2G19070 28.605 430 266 13 35 434 29 447 3.30e-47 169
MS.gene59166.t1 AT5G57840 28.182 440 274 11 17 432 11 432 1.51e-46 167
MS.gene59166.t1 AT1G03390 29.705 441 279 16 16 434 24 455 8.89e-46 165
MS.gene59166.t1 AT5G48930 29.452 438 271 14 17 434 11 430 2.18e-45 164
MS.gene59166.t1 AT1G28680 28.345 441 293 14 16 439 11 445 8.35e-45 162
MS.gene59166.t1 AT3G47170 26.899 487 279 17 3 438 6 466 5.02e-43 158
MS.gene59166.t1 AT1G28680 30.814 344 224 10 16 349 11 350 3.22e-41 151
MS.gene59166.t1 AT5G07080 28.298 470 273 16 1 435 6 446 1.89e-40 150
MS.gene59166.t1 AT2G23510 27.897 466 287 17 1 437 5 450 9.18e-36 138
MS.gene59166.t1 AT2G25150 28.105 459 293 16 5 439 9 454 1.95e-35 137
MS.gene59166.t1 AT1G78990 27.569 399 251 12 62 439 65 446 2.57e-32 128
MS.gene59166.t1 AT1G31490 25.432 405 256 13 7 387 4 386 5.21e-30 121
MS.gene59166.t1 AT5G07860 27.233 459 278 16 4 435 12 441 6.76e-29 118
MS.gene59166.t1 AT5G07870 27.093 454 262 17 15 428 24 448 5.28e-27 113
MS.gene59166.t1 AT1G65450 28.283 297 184 8 158 438 2 285 8.32e-27 109
MS.gene59166.t1 AT5G42830 27.912 455 246 17 15 428 23 436 1.48e-26 112
MS.gene59166.t1 AT5G07850 27.123 424 267 16 4 399 12 421 3.36e-26 110
MS.gene59166.t1 AT1G32910 26.173 405 256 15 62 439 63 451 8.18e-26 110
MS.gene59166.t1 AT3G50270 27.119 413 233 15 23 397 30 412 4.18e-25 107
MS.gene59166.t1 AT5G67150 25.513 439 263 18 23 430 30 435 1.18e-23 103
MS.gene59166.t1 AT3G26040 23.737 396 259 14 15 388 9 383 2.57e-23 102
MS.gene59166.t1 AT3G50280 26.382 398 252 17 23 397 28 407 6.27e-23 101
MS.gene59166.t1 AT4G31910 26.636 428 279 12 30 430 32 451 9.56e-23 100
MS.gene59166.t1 AT5G38130 25.628 398 253 14 23 397 48 425 1.51e-22 100
MS.gene59166.t1 AT1G24430 23.725 451 282 17 5 430 1 414 1.56e-22 100
MS.gene59166.t1 AT3G50300 26.685 356 222 13 64 392 59 402 4.18e-22 99.0
MS.gene59166.t1 AT1G24420 26.434 401 235 17 15 386 11 380 2.42e-21 96.7
MS.gene59166.t1 AT1G24420 26.434 401 235 17 15 386 11 380 2.42e-21 96.7
MS.gene59166.t1 AT5G23970 24.838 463 288 16 13 456 7 428 6.21e-21 95.1
MS.gene59166.t1 AT5G16410 25.575 348 224 12 62 388 89 422 1.40e-19 91.7
MS.gene59166.t1 AT5G47980 26.180 466 277 22 15 447 11 442 1.78e-18 87.8
MS.gene59166.t1 AT5G67160 25.917 409 240 15 58 435 50 426 4.59e-18 86.7
MS.gene59166.t1 AT3G30280 24.444 450 282 18 6 427 3 422 4.85e-17 83.6
MS.gene59166.t1 AT3G50280 25.641 273 185 10 138 397 10 277 7.08e-15 75.9
MS.gene59166.t1 AT5G23940 24.586 423 239 16 61 438 62 449 1.04e-14 76.6
MS.gene59166.t1 AT3G50290 26.022 269 160 8 138 382 16 269 7.37e-14 72.0
MS.gene59166.t1 AT5G47950 22.799 443 281 18 6 427 3 405 5.81e-13 70.9
MS.gene59166.t1 AT3G23840 27.136 199 120 6 80 269 81 263 1.12e-11 67.0

Find 108 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCATTGTTGGTGATTC+AGG 0.100165 1.4:-24706930 MS.gene59166:intron
TGCGGTGAGGATTTCGAAAT+TGG 0.188633 1.4:+24704364 None:intergenic
GGGTCTTTCCCTGCCATATT+TGG 0.199762 1.4:+24707190 None:intergenic
TTTGATGGTCTTACAAATTC+AGG 0.203685 1.4:+24707307 None:intergenic
GCATTCAGTTGCAGATTTAA+TGG 0.210614 1.4:-24704105 MS.gene59166:CDS
GGATTTGGAGATGTTGAATT+TGG 0.267773 1.4:-24704017 MS.gene59166:CDS
TTTGAAAACGTAACCCTTCT+TGG 0.271683 1.4:+24707242 None:intergenic
GCGATGGGCCATGTCATCTA+AGG 0.301591 1.4:+24704457 None:intergenic
CACCTAAGGCACTCATGAAT+TGG 0.305227 1.4:+24704618 None:intergenic
GATGTTAGTTTGAAAGAATT+TGG 0.347283 1.4:-24707028 MS.gene59166:CDS
GAAACAATGAAACTAGGTTT+TGG 0.349312 1.4:+24703786 None:intergenic
TTATAAGTATGATCCAAATA+TGG 0.357454 1.4:-24707203 MS.gene59166:CDS
TAAGGGGATAATTGTTCCTT+TGG 0.358085 1.4:+24704475 None:intergenic
GATGTGACACGTGCTGGATT+TGG 0.358389 1.4:-24704032 MS.gene59166:CDS
GAATGAACCTTCAATCTTAC+CGG 0.372906 1.4:-24704579 MS.gene59166:CDS
TGGAGATGTTGAATTTGGTT+GGG 0.375385 1.4:-24704012 MS.gene59166:CDS
TACAACCGCCGGGAAACTAA+TGG 0.379563 1.4:-24704195 MS.gene59166:CDS
TGCTAAAGGAGAGGAAGGTT+TGG 0.383515 1.4:-24703907 MS.gene59166:CDS
TTGGAGATGTTGAATTTGGT+TGG 0.391534 1.4:-24704013 MS.gene59166:CDS
AACATTAACGAACCTACTAT+TGG 0.395610 1.4:-24703813 MS.gene59166:CDS
AATGAGCGACGCAGCAGGTT+TGG 0.400466 1.4:-24704642 MS.gene59166:CDS
TGCAGATTTAATGGTTATCA+AGG 0.405426 1.4:-24704096 MS.gene59166:CDS
ATAATTGTTCCTTTGGTGTC+GGG 0.410344 1.4:+24704482 None:intergenic
AAATCCTCACCGCATGCCTT+TGG 0.410833 1.4:-24704355 MS.gene59166:CDS
CTGCTGCGTCGCTCATTGTA+TGG 0.414060 1.4:+24704648 None:intergenic
CGAACTTCTTCGTCAGTGTC+TGG 0.418327 1.4:+24704308 None:intergenic
AGGTCCTGCTAGGAAACTTA+TGG 0.433584 1.4:-24707095 MS.gene59166:CDS
GTCCAATTCATGAGTGCCTT+AGG 0.433988 1.4:-24704620 MS.gene59166:CDS
CCGGCGGTTGTAACCGCGAC+GGG 0.439479 1.4:+24704206 None:intergenic
TCACTGTGAATGATAGAGAT+TGG 0.444139 1.4:+24707341 None:intergenic
AGTGCTTAACTCTCCATTGT+TGG 0.450887 1.4:-24706939 MS.gene59166:CDS
CCCGTCGCGGTTACAACCGC+CGG 0.452094 1.4:-24704206 MS.gene59166:CDS
GCATTACCGTAGTAACCATT+TGG 0.454482 1.4:+24704239 None:intergenic
AAACTAATGGAAAATCCGTT+GGG 0.456426 1.4:-24704182 MS.gene59166:CDS
TACTTATAAAATTGTATCAC+TGG 0.463814 1.4:+24707217 None:intergenic
CACTGTGAATGATAGAGATT+GGG 0.466767 1.4:+24707342 None:intergenic
TCCATTTGCAGGTAGATTGA+AGG 0.477837 1.4:-24707119 MS.gene59166:CDS
CAATCTTACCGGTATGGCAT+AGG 0.482008 1.4:-24704568 MS.gene59166:CDS
TCCTTCAATCTACCTGCAAA+TGG 0.485704 1.4:+24707118 None:intergenic
GGAGATGTTGAATTTGGTTG+GGG 0.493359 1.4:-24704011 MS.gene59166:CDS
TTTAATCCACCGTTACCAAA+TGG 0.496653 1.4:-24704254 MS.gene59166:CDS
TTGATGGTCTTACAAATTCA+GGG 0.497631 1.4:+24707308 None:intergenic
TGCCATACCGGTAAGATTGA+AGG 0.498825 1.4:+24704572 None:intergenic
AAGTATGATCCAAATATGGC+AGG 0.501582 1.4:-24707199 MS.gene59166:CDS
CGAATTGTGGCTACCTCGGT+TGG 0.505273 1.4:+24704419 None:intergenic
AGCTCATCCAAACAAGGAAA+AGG 0.507667 1.4:+24706989 None:intergenic
CGATGAGTGCATGTTACTCT+TGG 0.517477 1.4:+24704521 None:intergenic
AACTTCTCCATGGCTTGAGC+TGG 0.517646 1.4:+24703867 None:intergenic
GAAACTAATGGAAAATCCGT+TGG 0.517685 1.4:-24704183 MS.gene59166:CDS
GATAATTGTTCCTTTGGTGT+CGG 0.520228 1.4:+24704481 None:intergenic
CGCTCATTGTATGGTTTAGA+CGG 0.527004 1.4:+24704657 None:intergenic
GTATCTGATGTGACACGTGC+TGG 0.527780 1.4:-24704038 MS.gene59166:CDS
TCCGCAAGAAATTTCACCTA+AGG 0.535116 1.4:+24704604 None:intergenic
GGAGGAGTTGGTGCTATACC+TGG 0.535378 1.4:-24703960 MS.gene59166:CDS
AACCTTCAATCTTACCGGTA+TGG 0.535791 1.4:-24704574 MS.gene59166:CDS
GCACCAACTCCTCCTTTAGC+TGG 0.539177 1.4:+24703969 None:intergenic
GTATATGGTGGACCAGCTAA+AGG 0.539427 1.4:-24703981 MS.gene59166:CDS
CTCTTTGACGAACTTCTCCA+TGG 0.543009 1.4:+24703857 None:intergenic
TGAGTGCATGTTACTCTTGG+TGG 0.546616 1.4:+24704524 None:intergenic
CAGACATAGATGACCAAGAA+GGG 0.546800 1.4:-24707255 MS.gene59166:CDS
AATCTTACCGGTATGGCATA+GGG 0.551315 1.4:-24704567 MS.gene59166:CDS
CGATGGGCCATGTCATCTAA+GGG 0.551926 1.4:+24704458 None:intergenic
TTGTTTACCAGCTCAAGCCA+TGG 0.552757 1.4:-24703874 MS.gene59166:CDS
AGAAGCTCCCTATGCCATAC+CGG 0.552944 1.4:+24704560 None:intergenic
GGACCAGCTAAAGGAGGAGT+TGG 0.553044 1.4:-24703972 MS.gene59166:CDS
ATAGGGAGCTTCTAAACGCA+AGG 0.556473 1.4:-24704550 MS.gene59166:CDS
CCGTCGCGGTTACAACCGCC+GGG 0.557301 1.4:-24704205 MS.gene59166:CDS
GCCTTAGGTGAAATTTCTTG+CGG 0.557407 1.4:-24704605 MS.gene59166:CDS
ATTAACGAACCTACTATTGG+TGG 0.562257 1.4:-24703810 MS.gene59166:CDS
AGCGCGAATTGTGGCTACCT+CGG 0.567132 1.4:+24704415 None:intergenic
AGTATGATCCAAATATGGCA+GGG 0.572294 1.4:-24707198 MS.gene59166:CDS
CCGTTACCAAATGGTTACTA+CGG 0.574521 1.4:-24704245 MS.gene59166:CDS
GATTGAAGGAAGGTCCTGCT+AGG 0.585694 1.4:-24707105 MS.gene59166:CDS
CCCGGCGGTTGTAACCGCGA+CGG 0.586843 1.4:+24704205 None:intergenic
AATACCTGAATCACCAACAA+TGG 0.589517 1.4:+24706926 None:intergenic
ATCTGTATTGTCAATGCTCG+TGG 0.590052 1.4:-24704281 MS.gene59166:CDS
GCAGATTTAATGGTTATCAA+GGG 0.590389 1.4:-24704095 MS.gene59166:CDS
ACATTAGTTTAAGGTCTACA+AGG 0.590944 1.4:+24703761 None:intergenic
TCCACCATAAGTTTCCTAGC+AGG 0.593970 1.4:+24707091 None:intergenic
CATACAATGAGCGACGCAGC+AGG 0.597595 1.4:-24704647 MS.gene59166:CDS
AACTCTAGTGCATACCCCAA+CGG 0.601820 1.4:+24704167 None:intergenic
AAGGAAGAAACAATGAAACT+AGG 0.603955 1.4:+24703780 None:intergenic
CCGTAGTAACCATTTGGTAA+CGG 0.610295 1.4:+24704245 None:intergenic
ATATAAAAGCTAGCAACACC+AGG 0.611199 1.4:+24703942 None:intergenic
TCAGACATAGATGACCAAGA+AGG 0.615146 1.4:-24707256 MS.gene59166:CDS
AATTATCCCCTTAGATGACA+TGG 0.615469 1.4:-24704465 MS.gene59166:CDS
TGGGAGAAGAGCGCGAATTG+TGG 0.616656 1.4:+24704406 None:intergenic
CTTCTAACCACAGTAAAATG+TGG 0.618528 1.4:+24704068 None:intergenic
AAAAGTAGCTCATCCAAACA+AGG 0.619216 1.4:+24706983 None:intergenic
TGATGGTCTTACAAATTCAG+GGG 0.623986 1.4:+24707309 None:intergenic
ATGGTGGATTGTAATGATGA+AGG 0.626655 1.4:-24707076 MS.gene59166:CDS
CGATTGTACGACAACGCCAA+AGG 0.629387 1.4:+24704339 None:intergenic
GAACAAGTTCCCGACACCAA+AGG 0.634936 1.4:-24704491 MS.gene59166:CDS
TCCTGCTAGGAAACTTATGG+TGG 0.635781 1.4:-24707092 MS.gene59166:CDS
GCTTGAGCTGGTAAACAAAG+TGG 0.635861 1.4:+24703879 None:intergenic
TCATCCAAACAAGGAAAAGG+AGG 0.640809 1.4:+24706992 None:intergenic
TAGTAACCATTTGGTAACGG+TGG 0.653193 1.4:+24704248 None:intergenic
TAGGTAACACGTCTCAAGTG+TGG 0.654765 1.4:-24704698 MS.gene59166:CDS
AACGCCAAAGGCATGCGGTG+AGG 0.656317 1.4:+24704351 None:intergenic
TTTGCAGGTAGATTGAAGGA+AGG 0.658164 1.4:-24707115 MS.gene59166:CDS
TAGGGAGCTTCTAAACGCAA+GGG 0.659865 1.4:-24704549 MS.gene59166:CDS
TATGGTGGACCAGCTAAAGG+AGG 0.665823 1.4:-24703978 MS.gene59166:CDS
TTAAGCACTTCTGAAGAACC+AGG 0.667440 1.4:+24706953 None:intergenic
GAGATGTTGAATTTGGTTGG+GGG 0.671180 1.4:-24704010 MS.gene59166:CDS
AACTAATGGAAAATCCGTTG+GGG 0.673325 1.4:-24704181 MS.gene59166:CDS
GTAACACGTCTCAAGTGTGG+TGG 0.692143 1.4:-24704695 MS.gene59166:CDS
GATGGGCCATGTCATCTAAG+GGG 0.704148 1.4:+24704459 None:intergenic
ACGACAACGCCAAAGGCATG+CGG 0.713965 1.4:+24704346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAGATAACTTTATATTA+TGG - chr1.4:24704412-24704431 MS.gene59166:CDS 10.0%
!! ATATTGAAACAATATTTAAA+GGG - chr1.4:24704559-24704578 MS.gene59166:CDS 10.0%
!! ATGAATTAATTAGTTAAAAT+TGG + chr1.4:24706356-24706375 None:intergenic 10.0%
!!! ATGAATTTTTATATCTTTTA+AGG - chr1.4:24706051-24706070 MS.gene59166:intron 10.0%
!!! ATTATTTTAGGTTATTATTT+TGG + chr1.4:24704833-24704852 None:intergenic 10.0%
!!! TAAAAAATTCTGATTTTTTT+TGG + chr1.4:24706013-24706032 None:intergenic 10.0%
!!! TAACCTAAAATAATTTTTTT+AGG - chr1.4:24704839-24704858 MS.gene59166:intron 10.0%
!!! TATTTCTTTTGAAAAATTAA+CGG + chr1.4:24704397-24704416 None:intergenic 10.0%
!!! TTTCCTAAAAAAATTATTTT+AGG + chr1.4:24704845-24704864 None:intergenic 10.0%
!! CATATTGAAACAATATTTAA+AGG - chr1.4:24704558-24704577 MS.gene59166:CDS 15.0%
!! TTATTTAATTGCATAAAAAG+TGG - chr1.4:24704946-24704965 MS.gene59166:intron 15.0%
!!! ATTATCACTTTTCATTTTTT+TGG - chr1.4:24705017-24705036 MS.gene59166:intron 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG - chr1.4:24706210-24706229 MS.gene59166:intron 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG + chr1.4:24705897-24705916 None:intergenic 15.0%
!!! TTATCACTTTTCATTTTTTT+GGG - chr1.4:24705018-24705037 MS.gene59166:intron 15.0%
!!! TTATTTTTCTCACTTTTTAT+TGG - chr1.4:24705548-24705567 MS.gene59166:intron 15.0%
!!! TTTTATGCAATTAAATAAGA+TGG + chr1.4:24704944-24704963 None:intergenic 15.0%
!! AAAAAAAATTAATTCAGCTG+AGG - chr1.4:24705298-24705317 MS.gene59166:intron 20.0%
!! ATATGAGTTTACATTAAAAG+AGG + chr1.4:24705929-24705948 None:intergenic 20.0%
!! CTTACATGTAAATTTGTAAA+TGG - chr1.4:24705046-24705065 MS.gene59166:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr1.4:24706270-24706289 MS.gene59166:intron 20.0%
!! GAAAAGTTGACATATTTATA+TGG + chr1.4:24706289-24706270 None:intergenic 20.0%
!! TACTTATAAAATTGTATCAC+TGG + chr1.4:24703908-24703927 None:intergenic 20.0%
!! TAGACAAAATTTATACATCA+CGG - chr1.4:24705736-24705755 MS.gene59166:intron 20.0%
!! TATATTTCACAAATTTACAG+TGG - chr1.4:24704264-24704283 MS.gene59166:CDS 20.0%
!! TATGAGTTTACATTAAAAGA+GGG + chr1.4:24705928-24705947 None:intergenic 20.0%
!! TATTCCTAGGATTTAAATAT+GGG + chr1.4:24704764-24704783 None:intergenic 20.0%
!! TGTGATTGTTATAAAAACAT+GGG + chr1.4:24706313-24706332 None:intergenic 20.0%
!! TTATAAGTATGATCCAAATA+TGG - chr1.4:24703919-24703938 MS.gene59166:CDS 20.0%
!! TTATTCCTAGGATTTAAATA+TGG + chr1.4:24704765-24704784 None:intergenic 20.0%
!! TTTAGGAAACACAAAATAAT+TGG - chr1.4:24704856-24704875 MS.gene59166:intron 20.0%
!!! AAATTTTCAATCACTACTTT+TGG + chr1.4:24706206-24706225 None:intergenic 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG - chr1.4:24705901-24705920 MS.gene59166:intron 20.0%
!!! AAGAAAATGTTTCCATTTTT+GGG - chr1.4:24704700-24704719 MS.gene59166:intron 20.0%
!!! CAAGCACAAATATATTTTTT+TGG - chr1.4:24705169-24705188 MS.gene59166:intron 20.0%
!!! CTTATCTCATATTGTTTTTA+GGG - chr1.4:24705794-24705813 MS.gene59166:intron 20.0%
!!! GAAAGTAATTTTATTTCATG+AGG + chr1.4:24703848-24703867 None:intergenic 20.0%
!!! GTAGTGATTGAAAATTTTAT+AGG - chr1.4:24706209-24706228 MS.gene59166:intron 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG + chr1.4:24705898-24705917 None:intergenic 20.0%
!!! TAAGAAAATGTTTCCATTTT+TGG - chr1.4:24704699-24704718 MS.gene59166:intron 20.0%
!!! TATCACTTTTCATTTTTTTG+GGG - chr1.4:24705019-24705038 MS.gene59166:intron 20.0%
!!! TCTTATCTCATATTGTTTTT+AGG - chr1.4:24705793-24705812 MS.gene59166:intron 20.0%
!!! TGGTACATATATGTTTTTAA+AGG - chr1.4:24705066-24705085 MS.gene59166:intron 20.0%
!!! TTATTCAACTAGTTAGATTT+TGG - chr1.4:24704507-24704526 MS.gene59166:CDS 20.0%
!!! TTTAGGGTTAAATATGTTTT+TGG - chr1.4:24705810-24705829 MS.gene59166:intron 20.0%
!!! TTTTTATTGGTCAAAAACAA+TGG - chr1.4:24705561-24705580 MS.gene59166:intron 20.0%
! AATATAGTACGAGTATATGT+TGG + chr1.4:24704368-24704387 None:intergenic 25.0%
! ATACCTTTCAAAAATGCTAA+AGG - chr1.4:24707201-24707220 MS.gene59166:CDS 25.0%
! ATATAGTACAAGTGTATGTT+CGG + chr1.4:24704321-24704340 None:intergenic 25.0%
! ATATAGTACGAGTATATGTT+GGG + chr1.4:24704367-24704386 None:intergenic 25.0%
! CATATACTCGTACTATATTA+AGG - chr1.4:24704369-24704388 MS.gene59166:CDS 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr1.4:24706269-24706288 MS.gene59166:intron 25.0%
! CTGTGATTGTTATAAAAACA+TGG + chr1.4:24706314-24706333 None:intergenic 25.0%
! TTTCTATCGAATAGAATCTT+AGG - chr1.4:24704804-24704823 MS.gene59166:intron 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG - chr1.4:24706238-24706257 MS.gene59166:intron 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr1.4:24706257-24706238 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG - chr1.4:24706239-24706258 MS.gene59166:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr1.4:24706258-24706239 None:intergenic 25.0%
!! CTTTTCTAATGATATCAACA+GGG + chr1.4:24703955-24703974 None:intergenic 25.0%
!! GATGTTAGTTTGAAAGAATT+TGG - chr1.4:24704094-24704113 MS.gene59166:CDS 25.0%
!! TTGATTGTAACAACACATTT+TGG + chr1.4:24705401-24705420 None:intergenic 25.0%
!!! AACCATTTTTTGACAACTTT+TGG + chr1.4:24705646-24705665 None:intergenic 25.0%
!!! AATCATTAACTTGTATCTTG+AGG + chr1.4:24704601-24704620 None:intergenic 25.0%
!!! AATTTTATTTCATGAGGTGT+TGG + chr1.4:24703842-24703861 None:intergenic 25.0%
!!! ATCACTTTTCATTTTTTTGG+GGG - chr1.4:24705020-24705039 MS.gene59166:intron 25.0%
!!! ATCATTAACTTGTATCTTGA+GGG + chr1.4:24704600-24704619 None:intergenic 25.0%
AAAACAATGGAGAGAGAAAA+AGG - chr1.4:24705574-24705593 MS.gene59166:intron 30.0%
AAACTAATGGAAAATCCGTT+GGG - chr1.4:24706940-24706959 MS.gene59166:CDS 30.0%
AACATTAACGAACCTACTAT+TGG - chr1.4:24707309-24707328 MS.gene59166:CDS 30.0%
AAGGAAGAAACAATGAAACT+AGG + chr1.4:24707345-24707364 None:intergenic 30.0%
AGATTTGTAAATGACACAAG+TGG - chr1.4:24705103-24705122 MS.gene59166:intron 30.0%
ATGACCCATATTTAAATCCT+AGG - chr1.4:24704757-24704776 MS.gene59166:intron 30.0%
GCAGATTTAATGGTTATCAA+GGG - chr1.4:24707027-24707046 MS.gene59166:CDS 30.0%
GTCCAAAAGTTGTCAAAAAA+TGG - chr1.4:24705641-24705660 MS.gene59166:intron 30.0%
TATATTTGTGCTTGAACTAC+AGG + chr1.4:24705163-24705182 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr1.4:24705523-24705542 None:intergenic 30.0%
TGCAGATTTAATGGTTATCA+AGG - chr1.4:24707026-24707045 MS.gene59166:CDS 30.0%
TTATAGGGACTAAAAGTTGA+AGG - chr1.4:24706225-24706244 MS.gene59166:intron 30.0%
TTATAGGGACTAAAAGTTGA+AGG + chr1.4:24706244-24706225 None:intergenic 30.0%
TTTCAAAAATGCTAAAGGAG+AGG - chr1.4:24707206-24707225 MS.gene59166:CDS 30.0%
! GAAACAATGAAACTAGGTTT+TGG + chr1.4:24707339-24707358 None:intergenic 30.0%
! GCTTTTCTAATGATATCAAC+AGG + chr1.4:24703956-24703975 None:intergenic 30.0%
! TCTCCTTTAGCATTTTTGAA+AGG + chr1.4:24707207-24707226 None:intergenic 30.0%
! TTGATGGTCTTACAAATTCA+GGG + chr1.4:24703817-24703836 None:intergenic 30.0%
! TTTGATGGTCTTACAAATTC+AGG + chr1.4:24703818-24703837 None:intergenic 30.0%
!! CTTGAATTTTGACCATTTGT+AGG - chr1.4:24706405-24706424 MS.gene59166:intron 30.0%
!! GTGTTTTATTATCCATTTGC+AGG - chr1.4:24703992-24704011 MS.gene59166:CDS 30.0%
!!! CGAAATTGGATTGTTTTTGT+TGG + chr1.4:24706747-24706766 None:intergenic 30.0%
AAAAATGCTAAAGGAGAGGA+AGG - chr1.4:24707210-24707229 MS.gene59166:CDS 35.0%
AAAAGTAGCTCATCCAAACA+AGG + chr1.4:24704142-24704161 None:intergenic 35.0%
AAATGATTCAAGTGAGGTCA+AGG + chr1.4:24705241-24705260 None:intergenic 35.0%
AACTAATGGAAAATCCGTTG+GGG - chr1.4:24706941-24706960 MS.gene59166:CDS 35.0%
AAGTATGATCCAAATATGGC+AGG - chr1.4:24703923-24703942 MS.gene59166:CDS 35.0%
AATACCTGAATCACCAACAA+TGG + chr1.4:24704199-24704218 None:intergenic 35.0%
AATGATTCAAGTGAGGTCAA+GGG + chr1.4:24705240-24705259 None:intergenic 35.0%
AATTATCCCCTTAGATGACA+TGG - chr1.4:24706657-24706676 MS.gene59166:intron 35.0%
AGACACACACTAGAGTTAAA+AGG + chr1.4:24705440-24705459 None:intergenic 35.0%
AGTATGATCCAAATATGGCA+GGG - chr1.4:24703924-24703943 MS.gene59166:CDS 35.0%
ATATAAAAGCTAGCAACACC+AGG + chr1.4:24707183-24707202 None:intergenic 35.0%
ATTAACGAACCTACTATTGG+TGG - chr1.4:24707312-24707331 MS.gene59166:CDS 35.0%
CACTGTGAATGATAGAGATT+GGG + chr1.4:24703783-24703802 None:intergenic 35.0%
CATGTTAAGCAACCCAAAAA+TGG + chr1.4:24704715-24704734 None:intergenic 35.0%
CTACACAAATGATTCAAGTG+AGG + chr1.4:24705247-24705266 None:intergenic 35.0%
CTCACTTGAATCATTTGTGT+AGG - chr1.4:24705245-24705264 MS.gene59166:intron 35.0%
CTTCTAACCACAGTAAAATG+TGG + chr1.4:24707057-24707076 None:intergenic 35.0%
GAAACTAATGGAAAATCCGT+TGG - chr1.4:24706939-24706958 MS.gene59166:CDS 35.0%
GAATGAACCTTCAATCTTAC+CGG - chr1.4:24706543-24706562 MS.gene59166:intron 35.0%
GCATTCAGTTGCAGATTTAA+TGG - chr1.4:24707017-24707036 MS.gene59166:CDS 35.0%
GGATTTGGAGATGTTGAATT+TGG - chr1.4:24707105-24707124 MS.gene59166:CDS 35.0%
TAAGGGGATAATTGTTCCTT+TGG + chr1.4:24706650-24706669 None:intergenic 35.0%
TCACTGTGAATGATAGAGAT+TGG + chr1.4:24703784-24703803 None:intergenic 35.0%
TGGAGATGTTGAATTTGGTT+GGG - chr1.4:24707110-24707129 MS.gene59166:CDS 35.0%
TTGGAGATGTTGAATTTGGT+TGG - chr1.4:24707109-24707128 MS.gene59166:CDS 35.0%
TTTAATCCACCGTTACCAAA+TGG - chr1.4:24706868-24706887 MS.gene59166:intron 35.0%
TTTGAAAACGTAACCCTTCT+TGG + chr1.4:24703883-24703902 None:intergenic 35.0%
TTTGTAAATGACACAAGTGG+AGG - chr1.4:24705106-24705125 MS.gene59166:intron 35.0%
! CTAGTTAGATTTTGGACTCA+TGG - chr1.4:24704515-24704534 MS.gene59166:CDS 35.0%
! GATAATTGTTCCTTTGGTGT+CGG + chr1.4:24706644-24706663 None:intergenic 35.0%
! TCTTGTTTCGTAGAAAGAGT+GGG - chr1.4:24705370-24705389 MS.gene59166:intron 35.0%
! TGATGGTCTTACAAATTCAG+GGG + chr1.4:24703816-24703835 None:intergenic 35.0%
! TGCAAGACACTTTTATTCCT+AGG + chr1.4:24704777-24704796 None:intergenic 35.0%
! TTCTTGTTTCGTAGAAAGAG+TGG - chr1.4:24705369-24705388 MS.gene59166:intron 35.0%
!! ATAATTGTTCCTTTGGTGTC+GGG + chr1.4:24706643-24706662 None:intergenic 35.0%
!! ATGGTGGATTGTAATGATGA+AGG - chr1.4:24704046-24704065 MS.gene59166:CDS 35.0%
!! TCATGTTAACTTGTGCTCTT+GGG + chr1.4:24704741-24704760 None:intergenic 35.0%
!!! ATTGTTTTTGTTGGTGAGGT+GGG + chr1.4:24706738-24706757 None:intergenic 35.0%
!!! CTTTTAAGATCAGATACGTG+TGG + chr1.4:24705330-24705349 None:intergenic 35.0%
!!! TGAAGGTGTTTTGTTCATTG+AGG - chr1.4:24704063-24704082 MS.gene59166:CDS 35.0%
!!! TTGGATTGTTTTTGTTGGTG+AGG + chr1.4:24706742-24706761 None:intergenic 35.0%
AACCTTCAATCTTACCGGTA+TGG - chr1.4:24706548-24706567 MS.gene59166:intron 40.0%
AATCTTACCGGTATGGCATA+GGG - chr1.4:24706555-24706574 MS.gene59166:intron 40.0%
ACCGAAGAAAAAAGAGCGAT+GGG + chr1.4:24706683-24706702 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr1.4:24705584-24705603 MS.gene59166:intron 40.0%
AGCTCATCCAAACAAGGAAA+AGG + chr1.4:24704136-24704155 None:intergenic 40.0%
AGTGCTTAACTCTCCATTGT+TGG - chr1.4:24704183-24704202 MS.gene59166:CDS 40.0%
ATCTGTATTGTCAATGCTCG+TGG - chr1.4:24706841-24706860 MS.gene59166:intron 40.0%
CAAGTGTATGTTCGGATTTG+CGG + chr1.4:24704313-24704332 None:intergenic 40.0%
CACAGCAAGTGTTTCATTTG+TGG - chr1.4:24706329-24706348 MS.gene59166:intron 40.0%
CAGACATAGATGACCAAGAA+GGG - chr1.4:24703867-24703886 MS.gene59166:CDS 40.0%
CCGTAGTAACCATTTGGTAA+CGG + chr1.4:24706880-24706899 None:intergenic 40.0%
CCGTTACCAAATGGTTACTA+CGG - chr1.4:24706877-24706896 MS.gene59166:intron 40.0%
CGCTCATTGTATGGTTTAGA+CGG + chr1.4:24706468-24706487 None:intergenic 40.0%
GAGATGTTGAATTTGGTTGG+GGG - chr1.4:24707112-24707131 MS.gene59166:CDS 40.0%
GCATTACCGTAGTAACCATT+TGG + chr1.4:24706886-24706905 None:intergenic 40.0%
GCCTTAGGTGAAATTTCTTG+CGG - chr1.4:24706517-24706536 MS.gene59166:intron 40.0%
GGAGATGTTGAATTTGGTTG+GGG - chr1.4:24707111-24707130 MS.gene59166:CDS 40.0%
GGATCAAGCTATTACATGGT+TGG - chr1.4:24705757-24705776 MS.gene59166:intron 40.0%
TCACGGATCAAGCTATTACA+TGG - chr1.4:24705753-24705772 MS.gene59166:intron 40.0%
TCAGACATAGATGACCAAGA+AGG - chr1.4:24703866-24703885 MS.gene59166:CDS 40.0%
TCATCCAAACAAGGAAAAGG+AGG + chr1.4:24704133-24704152 None:intergenic 40.0%
TCCATTTGCAGGTAGATTGA+AGG - chr1.4:24704003-24704022 MS.gene59166:CDS 40.0%
TCCGCAAGAAATTTCACCTA+AGG + chr1.4:24706521-24706540 None:intergenic 40.0%
TCCTTCAATCTACCTGCAAA+TGG + chr1.4:24704007-24704026 None:intergenic 40.0%
TGAGACGTGTTACCTACAAA+TGG + chr1.4:24706420-24706439 None:intergenic 40.0%
TTAAGATCAGATACGTGTGG+TGG + chr1.4:24705327-24705346 None:intergenic 40.0%
TTTGCAGGTAGATTGAAGGA+AGG - chr1.4:24704007-24704026 MS.gene59166:CDS 40.0%
! TCAACCTCCTTTTCCTTGTT+TGG - chr1.4:24704126-24704145 MS.gene59166:CDS 40.0%
! TTAAGCACTTCTGAAGAACC+AGG + chr1.4:24704172-24704191 None:intergenic 40.0%
!! ATTTTCCATTAGTTTCCCGG+CGG + chr1.4:24706935-24706954 None:intergenic 40.0%
!! CATGTTAACTTGTGCTCTTG+GGG + chr1.4:24704740-24704759 None:intergenic 40.0%
!! CGGATTTTCCATTAGTTTCC+CGG + chr1.4:24706938-24706957 None:intergenic 40.0%
!! GTCATGTTAACTTGTGCTCT+TGG + chr1.4:24704742-24704761 None:intergenic 40.0%
!! TAGTAACCATTTGGTAACGG+TGG + chr1.4:24706877-24706896 None:intergenic 40.0%
!!! GAGCTACTTTTTGATGTTCC+TGG - chr1.4:24704151-24704170 MS.gene59166:CDS 40.0%
!!! GATTGTTTTTGTTGGTGAGG+TGG + chr1.4:24706739-24706758 None:intergenic 40.0%
!!! TCATGAGGTGTTGGTTTTGA+TGG + chr1.4:24703833-24703852 None:intergenic 40.0%
AACTCTAGTGCATACCCCAA+CGG + chr1.4:24706958-24706977 None:intergenic 45.0%
AGGTCCTGCTAGGAAACTTA+TGG - chr1.4:24704027-24704046 MS.gene59166:CDS 45.0%
ATAGGGAGCTTCTAAACGCA+AGG - chr1.4:24706572-24706591 MS.gene59166:intron 45.0%
CAATCTTACCGGTATGGCAT+AGG - chr1.4:24706554-24706573 MS.gene59166:intron 45.0%
CGAGTATATGTTGGGATGAG+TGG + chr1.4:24704359-24704378 None:intergenic 45.0%
CGATGAGTGCATGTTACTCT+TGG + chr1.4:24706604-24706623 None:intergenic 45.0%
CTCTTTGACGAACTTCTCCA+TGG + chr1.4:24707268-24707287 None:intergenic 45.0%
GACCGAAGAAAAAAGAGCGA+TGG + chr1.4:24706684-24706703 None:intergenic 45.0%
GCTTGAGCTGGTAAACAAAG+TGG + chr1.4:24707246-24707265 None:intergenic 45.0%
GTCCAATTCATGAGTGCCTT+AGG - chr1.4:24706502-24706521 MS.gene59166:intron 45.0%
GTTTCGTAGAAAGAGTGGGT+GGG - chr1.4:24705374-24705393 MS.gene59166:intron 45.0%
TAGGGAGCTTCTAAACGCAA+GGG - chr1.4:24706573-24706592 MS.gene59166:intron 45.0%
TAGGTAACACGTCTCAAGTG+TGG - chr1.4:24706424-24706443 MS.gene59166:intron 45.0%
TCCACCATAAGTTTCCTAGC+AGG + chr1.4:24704034-24704053 None:intergenic 45.0%
TCCTGCTAGGAAACTTATGG+TGG - chr1.4:24704030-24704049 MS.gene59166:CDS 45.0%
TGAGTGCATGTTACTCTTGG+TGG + chr1.4:24706601-24706620 None:intergenic 45.0%
TGCCATACCGGTAAGATTGA+AGG + chr1.4:24706553-24706572 None:intergenic 45.0%
TGCGGTGAGGATTTCGAAAT+TGG + chr1.4:24706761-24706780 None:intergenic 45.0%
TGCTAAAGGAGAGGAAGGTT+TGG - chr1.4:24707215-24707234 MS.gene59166:CDS 45.0%
TGTTTCGTAGAAAGAGTGGG+TGG - chr1.4:24705373-24705392 MS.gene59166:intron 45.0%
TTGTTTACCAGCTCAAGCCA+TGG - chr1.4:24707248-24707267 MS.gene59166:CDS 45.0%
! CACCTAAGGCACTCATGAAT+TGG + chr1.4:24706507-24706526 None:intergenic 45.0%
! GGGTAGACCACATTTTACTG+TGG - chr1.4:24707047-24707066 MS.gene59166:CDS 45.0%
!! CTCTCCATTGTTGGTGATTC+AGG - chr1.4:24704192-24704211 MS.gene59166:CDS 45.0%
!! GTATATGGTGGACCAGCTAA+AGG - chr1.4:24707141-24707160 MS.gene59166:CDS 45.0%
!!! GCCCATCGCTCTTTTTTCTT+CGG - chr1.4:24706679-24706698 MS.gene59166:intron 45.0%
!!! GGTTTTGGACCACCAATAGT+AGG + chr1.4:24707324-24707343 None:intergenic 45.0%
!!! TTTTTTCTTCGGTCCAACCG+AGG - chr1.4:24706690-24706709 MS.gene59166:intron 45.0%
!! ACAAAAATTAAATTAAAAAT+AGG - chr1.4:24706178-24706197 MS.gene59166:intron 5.0%
!! CAAAAATTAAATTAAAAATA+GGG - chr1.4:24706179-24706198 MS.gene59166:intron 5.0%
!!! AAAAAATTCTAATTTTTTTT+AGG - chr1.4:24706105-24706124 MS.gene59166:intron 5.0%
!!! TATAATATTTTTAATTTTTC+TGG + chr1.4:24705981-24706000 None:intergenic 5.0%
AAATCCTCACCGCATGCCTT+TGG - chr1.4:24706767-24706786 MS.gene59166:intron 50.0%
AACTTCTCCATGGCTTGAGC+TGG + chr1.4:24707258-24707277 None:intergenic 50.0%
AGAAGCTCCCTATGCCATAC+CGG + chr1.4:24706565-24706584 None:intergenic 50.0%
CGAACTTCTTCGTCAGTGTC+TGG + chr1.4:24706817-24706836 None:intergenic 50.0%
CGATGGGCCATGTCATCTAA+GGG + chr1.4:24706667-24706686 None:intergenic 50.0%
CGATTGTACGACAACGCCAA+AGG + chr1.4:24706786-24706805 None:intergenic 50.0%
GAACAAGTTCCCGACACCAA+AGG - chr1.4:24706631-24706650 MS.gene59166:intron 50.0%
GATGGGCCATGTCATCTAAG+GGG + chr1.4:24706666-24706685 None:intergenic 50.0%
GATGTGACACGTGCTGGATT+TGG - chr1.4:24707090-24707109 MS.gene59166:CDS 50.0%
GATTGAAGGAAGGTCCTGCT+AGG - chr1.4:24704017-24704036 MS.gene59166:CDS 50.0%
GGGTCTTTCCCTGCCATATT+TGG + chr1.4:24703935-24703954 None:intergenic 50.0%
GGTTGGGGGAAAGCTGTATA+TGG - chr1.4:24707126-24707145 MS.gene59166:CDS 50.0%
GTAACACGTCTCAAGTGTGG+TGG - chr1.4:24706427-24706446 MS.gene59166:intron 50.0%
GTATCTGATGTGACACGTGC+TGG - chr1.4:24707084-24707103 MS.gene59166:CDS 50.0%
TACAACCGCCGGGAAACTAA+TGG - chr1.4:24706927-24706946 MS.gene59166:intron 50.0%
TGGGGGAAAGCTGTATATGG+TGG - chr1.4:24707129-24707148 MS.gene59166:CDS 50.0%
! TGAGGTCAAGGGTGTGTTTG+AGG + chr1.4:24705229-24705248 None:intergenic 50.0%
! TGCTTTTGCATTTCCCGTCG+CGG - chr1.4:24706903-24706922 MS.gene59166:intron 50.0%
!! TATGGTGGACCAGCTAAAGG+AGG - chr1.4:24707144-24707163 MS.gene59166:CDS 50.0%
AATGAGCGACGCAGCAGGTT+TGG - chr1.4:24706480-24706499 MS.gene59166:intron 55.0%
AGCGCGAATTGTGGCTACCT+CGG + chr1.4:24706710-24706729 None:intergenic 55.0%
CATACAATGAGCGACGCAGC+AGG - chr1.4:24706475-24706494 MS.gene59166:intron 55.0%
CGAATTGTGGCTACCTCGGT+TGG + chr1.4:24706706-24706725 None:intergenic 55.0%
CTGCTGCGTCGCTCATTGTA+TGG + chr1.4:24706477-24706496 None:intergenic 55.0%
GCACCAACTCCTCCTTTAGC+TGG + chr1.4:24707156-24707175 None:intergenic 55.0%
GCGATGGGCCATGTCATCTA+AGG + chr1.4:24706668-24706687 None:intergenic 55.0%
GGACCAGCTAAAGGAGGAGT+TGG - chr1.4:24707150-24707169 MS.gene59166:CDS 55.0%
! ACGACAACGCCAAAGGCATG+CGG + chr1.4:24706779-24706798 None:intergenic 55.0%
! TGGGAGAAGAGCGCGAATTG+TGG + chr1.4:24706719-24706738 None:intergenic 55.0%
!! GGAGGAGTTGGTGCTATACC+TGG - chr1.4:24707162-24707181 MS.gene59166:CDS 55.0%
! AACGCCAAAGGCATGCGGTG+AGG + chr1.4:24706774-24706793 None:intergenic 60.0%
CCCGGCGGTTGTAACCGCGA+CGG + chr1.4:24706920-24706939 None:intergenic 70.0%
CCCGTCGCGGTTACAACCGC+CGG - chr1.4:24706916-24706935 MS.gene59166:intron 70.0%
CCGGCGGTTGTAACCGCGAC+GGG + chr1.4:24706919-24706938 None:intergenic 70.0%
CCGTCGCGGTTACAACCGCC+GGG - chr1.4:24706917-24706936 MS.gene59166:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 24703776 24707368 24703776 ID=MS.gene59166
chr1.4 mRNA 24703776 24707368 24703776 ID=MS.gene59166.t1;Parent=MS.gene59166
chr1.4 exon 24706931 24707368 24706931 ID=MS.gene59166.t1.exon1;Parent=MS.gene59166.t1
chr1.4 CDS 24706931 24707368 24706931 ID=cds.MS.gene59166.t1;Parent=MS.gene59166.t1
chr1.4 exon 24703776 24704717 24703776 ID=MS.gene59166.t1.exon2;Parent=MS.gene59166.t1
chr1.4 CDS 24703776 24704717 24703776 ID=cds.MS.gene59166.t1;Parent=MS.gene59166.t1
Gene Sequence

>MS.gene59166

ATGGCCCAATCTCTATCATTCACAGTGAAGAGAAGTGCCCCTGAATTTGTAAGACCATCAAAACCAACACCTCATGAAATAAAATTACTTTCAGACATAGATGACCAAGAAGGGTTACGTTTTCAAATTCCAGTGATACAATTTTATAAGTATGATCCAAATATGGCAGGGAAAGACCCTGTTGATATCATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGATTGAAGGAAGGTCCTGCTAGGAAACTTATGGTGGATTGTAATGATGAAGGTGTTTTGTTCATTGAGGCTGATGCTGATGTTAGTTTGAAAGAATTTGGTGATGCTCTTCAACCTCCTTTTCCTTGTTTGGATGAGCTACTTTTTGATGTTCCTGGTTCTTCAGAAGTGCTTAACTCTCCATTGTTGGTGATTCAGGTATTGTATTGATGTTCTTAATGCATAGTTATTTTGACAAAGCTATACTTTATATTTCACAAATTTACAGTGGTTCATCTTGATTTTTCAATTTCACCGCAAATCCGAACATACACTTGTACTATATGATATTTCAATTTCACCACTCATCCCAACATATACTCGTACTATATTAAGGATCCGTTAATTTTTCAAAAGAAATAGATAACTTTATATTATGGATTATTTGAAAGACTTTCAAGTCAATAAATATATATAAAATTAATAGACTACATGATTATAAAAATAATTAATTATTCAACTAGTTAGATTTTGGACTCATGGAAGAAGTTAGACTTTCACATCATATTGAAACAATATTTAAAGGGAAATGTTAACTTGTGCCCTCAAGATACAAGTTAATGATTCAAAATAGAAATATTCGACTGTAATTAATGCATTTGACTTTAATTTTTTTACTTTTCAATAATAGAATGCACAAAATTTAAGAAAATGTTTCCATTTTTGGGTTGCTTAACATGTGCCCCAAGAGCACAAGTTAACATGACCCATATTTAAATCCTAGGAATAAAAGTGTCTTGCATTTAGTGTTTCTATCGAATAGAATCTTAGGAAACCAAAATAATAACCTAAAATAATTTTTTTAGGAAACACAAAATAATTGGTTATTCACAATTATTATTAAGAATATCGTTTAAAAGTTCAAATTTGTTATTTATATTAGCTACCATCTTATTTAATTGCATAAAAAGTGGACATTTAATTTGAAAATATGAATATTAAATTAAAGACATCTTTAATACATTATCACTTTTCATTTTTTTGGGGGAAACTTACATGTAAATTTGTAAATGGTACATATATGTTTTTAAAGGTATAAATGACACATAGATTTGTAAATGACACAAGTGGAGGATGAAGAGATAGCATTAGTGAAAATTTGCAACCTGTAGTTCAAGCACAAATATATTTTTTTGGATCAAGATTATTTTTATTGATGCTGCGTAGAATTCCTCAAACACACCCTTGACCTCACTTGAATCATTTGTGTAGGTTAAAATATACTTTTTCTTTTATCTTGAACAAAAAAAATTAATTCAGCTGAGGATACCACCACACGTATCTGATCTTAAAAGTATGTTTGCAATAACTTTATTCTTGTTTCGTAGAAAGAGTGGGTGGGACCAAAATGTGTTGTTACAATCAATCAATTAATACTTGTTCCTTTTAACTCTAGTGTGTGTCTCAATTTTAAAAATGAAATGTTCATTTGTTATATCTTTCTTTAGTAATGATATTTTGACATCCATTTGATGACAACTTTGTTGACAACTTTATTTTTCTCACTTTTTATTGGTCAAAAACAATGGAGAGAGAAAAAGGAAGAGAGAGGATAACAACATCATGTGAGTATGAGAGAGAAAATTGTCCAAAAGTTGTCAAAAAATGGTTGTCAAAATATCATTTCTCATCTTTCTTTTAGCTGACTTTTATTCATCGTTTAATTAGAATGAATCACAATTAGACAAAATTTATACATCACGGATCAAGCTATTACATGGTTGGTATGAAGTCAATGTCTTATCTCATATTGTTTTTAGGGTTAAATATGTTTTTGGTCCATATAAATATGTCAACTTTTCGTTTTAATCCCTCTAAAATTTTCCTTCAACTTTTAGTCCCTATAAAATTTTCAATCTTCACTTTTGGTCCCTCTTTTAATGTAAACTCATATGTAGAATTCATATAATTTAATAAAATTTTCCAGAAAAATTAAAAATATTATAAGAATCACTCCAAAAAAAATCAGAATTTTTTAACAAAACATGAATTTAATATGAATTTTTATATCTTTTAAGGTTAAAAATTCATATTTAATTTGTGTTTTGTTAAAAAATTCTAATTTTTTTTAGGAAATTTATTCAGAATATTCTACACATTTCTGCACAATTTCATTGAAAAATACAAAAATTAAATTAAAAATAGGGACCAAAAGTAGTGATTGAAAATTTTATAGGGACTAAAAGTTGAAGGAAAATTTTAGAGGGACTAAAACGAAAAGTTGACATATTTATAGGGACTAAAAACATATTTAACCCATGTTTTTATAACAATCACAGCAAGTGTTTCATTTGTGGTCCAATTTTAACTAATTAATTCATGAAAATAAAATTATTCATTCATTCACATACTTGAATTTTGACCATTTGTAGGTAACACGTCTCAAGTGTGGTGGTTTCATTTTTGCTCTCCGTCTAAACCATACAATGAGCGACGCAGCAGGTTTGGTCCAATTCATGAGTGCCTTAGGTGAAATTTCTTGCGGAATGAATGAACCTTCAATCTTACCGGTATGGCATAGGGAGCTTCTAAACGCAAGGGATCCACCAAGAGTAACATGCACTCATCGTGAATATGAACAAGTTCCCGACACCAAAGGAACAATTATCCCCTTAGATGACATGGCCCATCGCTCTTTTTTCTTCGGTCCAACCGAGGTAGCCACAATTCGCGCTCTTCTCCCACCTCACCAACAAAAACAATCCAATTTCGAAATCCTCACCGCATGCCTTTGGCGTTGTCGTACAATCGCGTTACAACCAGACACTGACGAAGAAGTTCGCATAATCTGTATTGTCAATGCTCGTGGCAAGTTTAATCCACCGTTACCAAATGGTTACTACGGTAATGCTTTTGCATTTCCCGTCGCGGTTACAACCGCCGGGAAACTAATGGAAAATCCGTTGGGGTATGCACTAGAGTTAGTTAAAAAAGCAAAATCTGATGTAACTCAAGAATACATGCATTCAGTTGCAGATTTAATGGTTATCAAGGGTAGACCACATTTTACTGTGGTTAGAAGTTATCTTGTATCTGATGTGACACGTGCTGGATTTGGAGATGTTGAATTTGGTTGGGGGAAAGCTGTATATGGTGGACCAGCTAAAGGAGGAGTTGGTGCTATACCTGGTGTTGCTAGCTTTTATATACCTTTCAAAAATGCTAAAGGAGAGGAAGGTTTGGTTATACCACTTTGTTTACCAGCTCAAGCCATGGAGAAGTTCGTCAAAGAGTTAGATAGTGTGCTTAAGAACAACATTAACGAACCTACTATTGGTGGTCCAAAACCTAGTTTCATTGTTTCTTCCTTGTAG
Protein sequence

>MS.gene59166.t1

MAQSLSFTVKRSAPEFVRPSKPTPHEIKLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKALAKTLVFYYPFAGRLKEGPARKLMVDCNDEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNSPLLVIQVTRLKCGGFIFALRLNHTMSDAAGLVQFMSALGEISCGMNEPSILPVWHRELLNARDPPRVTCTHREYEQVPDTKGTIIPLDDMAHRSFFFGPTEVATIRALLPPHQQKQSNFEILTACLWRCRTIALQPDTDEEVRIICIVNARGKFNPPLPNGYYGNAFAFPVAVTTAGKLMENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRSYLVSDVTRAGFGDVEFGWGKAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKELDSVLKNNINEPTIGGPKPSFIVSSL