AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59169

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59169.t1 MTR_1g040315 86.811 417 55 0 1 417 1 417 0.0 748
MS.gene59169.t1 MTR_1g040195 69.348 460 74 5 1 417 1 436 0.0 621
MS.gene59169.t1 MTR_1g040215 64.208 461 121 4 1 417 1 461 0.0 589
MS.gene59169.t1 MTR_1g040175 61.388 461 131 5 1 417 1 458 0.0 550
MS.gene59169.t1 MTR_1g040320 58.261 460 114 4 1 417 1 425 6.77e-179 505
MS.gene59169.t1 MTR_5g076700 51.948 462 166 10 5 417 6 460 6.30e-155 446
MS.gene59169.t1 MTR_7g114470 49.460 463 177 9 5 417 7 462 8.20e-149 430
MS.gene59169.t1 MTR_7g114560 49.351 462 177 11 5 417 6 459 5.33e-148 428
MS.gene59169.t1 MTR_1g081660 53.303 439 156 9 4 396 13 448 2.11e-146 424
MS.gene59169.t1 MTR_7g114550 48.780 451 178 9 2 404 3 448 8.35e-146 422
MS.gene59169.t1 MTR_7g114520 47.495 459 192 8 5 417 7 462 3.57e-140 408
MS.gene59169.t1 MTR_7g114490 47.907 430 176 7 5 388 6 433 2.21e-136 400
MS.gene59169.t1 MTR_0042s0190 54.882 297 90 4 165 417 1 297 3.77e-105 313
MS.gene59169.t1 MTR_0502s0010 58.667 225 81 6 100 316 1 221 3.21e-83 255
MS.gene59169.t1 MTR_0042s0230 76.871 147 34 0 1 147 1 147 5.79e-77 236
MS.gene59169.t1 MTR_0042s0190 53.070 228 63 4 234 417 5 232 4.28e-72 226
MS.gene59169.t1 MTR_7g114510 42.947 319 130 10 146 417 7 320 1.43e-70 225
MS.gene59169.t1 MTR_8g039950 78.906 128 27 0 1 128 1 128 9.49e-68 211
MS.gene59169.t1 MTR_7g114420 42.053 302 92 11 165 417 1 268 2.93e-60 197
MS.gene59169.t1 MTR_8g096530 31.566 396 220 14 1 353 1 388 3.21e-55 189
MS.gene59169.t1 MTR_7g114500 55.102 147 66 0 5 151 6 152 4.98e-55 179
MS.gene59169.t1 MTR_3g098980 30.120 415 239 11 17 388 22 428 1.11e-54 187
MS.gene59169.t1 MTR_3g051790 55.479 146 65 0 2 147 6 151 1.71e-50 169
MS.gene59169.t1 MTR_7g027175 30.263 380 218 10 16 353 23 397 5.17e-50 175
MS.gene59169.t1 MTR_1g053315 28.864 440 251 16 17 396 26 463 7.97e-41 151
MS.gene59169.t1 MTR_4g055170 26.667 450 265 11 4 393 9 453 8.85e-39 145
MS.gene59169.t1 MTR_3g035000 39.583 240 75 7 195 387 39 255 1.44e-38 141
MS.gene59169.t1 MTR_7g110610 26.904 394 197 11 5 358 1 343 3.70e-36 137
MS.gene59169.t1 MTR_2g105330 27.231 437 253 16 5 388 1 425 1.06e-35 136
MS.gene59169.t1 MTR_2g064495 27.294 425 246 15 17 388 11 425 3.00e-34 132
MS.gene59169.t1 MTR_8g075610 28.046 435 234 15 17 392 2 416 3.02e-34 132
MS.gene59169.t1 MTR_4g007540 27.295 403 228 13 41 388 34 426 1.35e-32 128
MS.gene59169.t1 MTR_3g112180 27.577 388 226 10 17 357 20 399 1.79e-32 128
MS.gene59169.t1 MTR_7g085930 30.154 325 186 12 16 311 13 325 1.16e-30 123
MS.gene59169.t1 MTR_3g071320 27.107 439 255 17 8 391 6 434 1.79e-28 116
MS.gene59169.t1 MTR_4g013050 26.498 434 214 13 17 388 12 402 8.90e-28 114
MS.gene59169.t1 MTR_7g085990 29.012 324 189 11 15 309 8 319 9.62e-28 114
MS.gene59169.t1 MTR_4g088765 67.123 73 24 0 1 73 1 73 1.41e-26 103
MS.gene59169.t1 MTR_7g084940 24.872 390 247 9 5 352 12 397 1.51e-26 111
MS.gene59169.t1 MTR_6g053810 26.300 327 206 12 3 304 5 321 1.81e-26 111
MS.gene59169.t1 MTR_7g084910 25.858 379 237 10 8 352 16 384 8.18e-26 108
MS.gene59169.t1 MTR_7g086000 27.387 398 198 19 61 380 57 441 3.07e-25 107
MS.gene59169.t1 MTR_3g465930 27.414 321 201 11 7 304 8 319 4.97e-25 107
MS.gene59169.t1 MTR_3g465910 26.791 321 200 11 7 304 8 316 7.13e-24 103
MS.gene59169.t1 MTR_3g465990 26.852 324 200 12 4 304 7 316 8.31e-24 103
MS.gene59169.t1 MTR_3g465980 26.012 346 217 13 5 325 8 339 4.23e-23 101
MS.gene59169.t1 MTR_3g465950 29.104 268 163 8 56 304 60 319 1.66e-22 99.8
MS.gene59169.t1 MTR_2g015430 26.126 444 261 19 6 392 1 434 2.95e-22 98.6
MS.gene59169.t1 MTR_7g056087 26.380 326 203 11 17 316 12 326 4.49e-22 98.2
MS.gene59169.t1 MTR_3g026950 23.342 407 235 11 17 353 10 409 6.64e-21 94.7
MS.gene59169.t1 MTR_4g127650 25.962 312 208 10 8 303 9 313 1.35e-20 94.0
MS.gene59169.t1 MTR_8g079980 24.036 441 248 18 15 393 9 424 1.55e-20 93.6
MS.gene59169.t1 MTR_3g465880 28.754 313 191 11 16 304 17 321 1.06e-19 91.3
MS.gene59169.t1 MTR_3g465870 27.483 302 191 11 5 287 8 300 3.06e-19 89.7
MS.gene59169.t1 MTR_4g097090 24.458 323 214 9 1 303 1 313 6.94e-19 88.6
MS.gene59169.t1 MTR_8g079970 24.281 313 198 11 15 309 9 300 3.39e-18 86.7
MS.gene59169.t1 MTR_5g084870 23.377 308 204 12 15 309 9 297 6.54e-18 85.9
MS.gene59169.t1 MTR_7g026860 25.118 422 237 16 23 386 25 425 2.21e-17 84.0
MS.gene59169.t1 MTR_8g079950 29.050 179 111 5 15 187 9 177 1.21e-16 82.0
MS.gene59169.t1 MTR_3g049150 24.752 303 209 10 17 305 10 307 2.76e-16 80.9
MS.gene59169.t1 MTR_7g114417 67.308 52 16 1 89 139 29 80 3.16e-16 73.9
MS.gene59169.t1 MTR_8g041770 26.885 305 187 11 23 311 31 315 3.86e-16 80.5
MS.gene59169.t1 MTR_3g101960 29.927 274 166 13 58 314 62 326 4.34e-16 80.1
MS.gene59169.t1 MTR_8g467590 23.160 462 258 19 12 401 6 442 5.10e-16 80.1
MS.gene59169.t1 MTR_5g090880 25.342 292 181 11 41 308 49 327 6.31e-16 79.7
MS.gene59169.t1 MTR_4g094842 26.165 279 177 11 48 305 46 316 1.46e-15 78.6
MS.gene59169.t1 MTR_0190s0020 28.231 294 183 11 23 303 30 308 1.63e-15 78.6
MS.gene59169.t1 MTR_3g101930 29.304 273 161 13 64 314 70 332 3.50e-15 77.4
MS.gene59169.t1 MTR_5g090830 25.402 311 191 11 29 314 37 331 5.38e-15 77.0
MS.gene59169.t1 MTR_4g022800 27.044 318 165 12 23 305 25 310 1.82e-14 75.1
MS.gene59169.t1 MTR_5g090810 24.204 314 191 11 29 314 37 331 2.04e-14 75.1
MS.gene59169.t1 MTR_3g450690 28.378 222 126 7 64 268 72 277 3.76e-14 74.3
MS.gene59169.t1 MTR_4g022720 25.581 301 185 13 23 305 25 304 3.97e-14 74.3
MS.gene59169.t1 MTR_3g450710 26.978 278 154 11 64 308 65 326 5.57e-14 73.9
MS.gene59169.t1 MTR_4g022760 27.215 316 167 12 23 305 25 310 5.64e-14 73.6
MS.gene59169.t1 MTR_4g094835 26.042 288 174 10 51 314 51 323 1.10e-13 72.8
MS.gene59169.t1 MTR_8g079710 31.868 182 101 6 23 196 30 196 2.09e-13 72.0
MS.gene59169.t1 MTR_8g467580 22.350 434 239 18 14 375 8 415 2.34e-13 72.0
MS.gene59169.t1 MTR_4g094832 30.603 232 127 10 94 307 85 300 2.99e-13 71.6
MS.gene59169.t1 MTR_3g101970 26.936 297 187 13 29 314 36 313 3.40e-13 71.2
MS.gene59169.t1 MTR_3g088420 26.780 295 183 12 23 303 25 300 3.52e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59169.t1 AT5G17540 50.000 466 179 11 1 417 1 461 1.64e-147 427
MS.gene59169.t1 AT3G03480 48.409 440 176 10 5 397 15 450 4.77e-132 387
MS.gene59169.t1 AT5G41040 31.250 384 209 12 16 352 40 415 4.12e-54 186
MS.gene59169.t1 AT5G41040 31.250 384 209 12 16 352 24 399 4.75e-54 186
MS.gene59169.t1 AT3G48720 27.842 431 261 11 1 388 1 424 2.90e-53 183
MS.gene59169.t1 AT5G63560 29.452 438 251 14 1 393 1 425 1.02e-52 182
MS.gene59169.t1 AT1G27620 27.251 422 242 12 16 388 16 421 2.84e-36 138
MS.gene59169.t1 AT3G62160 29.863 365 192 14 57 369 48 400 1.52e-35 136
MS.gene59169.t1 AT1G65450 27.333 450 261 15 1 396 12 449 7.29e-35 134
MS.gene59169.t1 AT5G57840 25.785 446 255 11 7 390 1 432 2.15e-34 133
MS.gene59169.t1 AT5G48930 27.696 408 230 14 40 392 33 430 3.03e-33 129
MS.gene59169.t1 AT2G19070 25.450 444 255 13 17 392 12 447 7.07e-32 126
MS.gene59169.t1 AT3G47170 26.933 375 221 15 7 344 10 368 1.01e-30 123
MS.gene59169.t1 AT1G03390 28.188 447 236 17 16 392 24 455 9.61e-30 120
MS.gene59169.t1 AT1G28680 25.167 449 259 15 14 397 9 445 2.70e-29 119
MS.gene59169.t1 AT2G40230 28.133 391 212 14 17 351 13 390 2.82e-29 119
MS.gene59169.t1 AT1G28680 28.065 310 191 11 14 303 9 306 5.78e-28 114
MS.gene59169.t1 AT5G07080 29.664 327 197 11 9 314 14 328 1.44e-26 111
MS.gene59169.t1 AT2G23510 24.573 468 258 14 1 395 5 450 2.16e-26 110
MS.gene59169.t1 AT2G25150 24.000 450 273 15 9 397 13 454 2.49e-24 105
MS.gene59169.t1 AT1G31490 29.104 268 154 10 17 268 14 261 2.04e-22 99.4
MS.gene59169.t1 AT1G78990 26.055 403 210 16 62 397 65 446 1.43e-20 94.0
MS.gene59169.t1 AT3G26040 23.501 417 246 15 15 375 9 408 6.86e-19 88.6
MS.gene59169.t1 AT4G31910 28.428 299 185 9 43 314 43 339 4.75e-18 86.3
MS.gene59169.t1 AT1G24430 23.367 398 229 15 43 388 41 414 1.17e-17 85.1
MS.gene59169.t1 AT1G32910 25.926 405 215 18 62 397 63 451 2.87e-17 84.0
MS.gene59169.t1 AT5G47980 24.891 458 251 22 15 405 11 442 4.23e-17 83.2
MS.gene59169.t1 AT1G24420 27.796 313 180 13 15 307 11 297 6.53e-17 82.8
MS.gene59169.t1 AT1G24420 27.796 313 180 13 15 307 11 297 6.53e-17 82.8
MS.gene59169.t1 AT5G07850 27.809 356 201 16 16 345 15 340 1.27e-16 82.0
MS.gene59169.t1 AT4G15390 25.632 277 178 12 3 269 2 260 1.07e-15 79.0
MS.gene59169.t1 AT4G15390 25.632 277 178 12 3 269 2 260 1.07e-15 79.0
MS.gene59169.t1 AT5G07860 24.211 380 228 13 64 393 72 441 2.97e-15 77.8
MS.gene59169.t1 AT3G30280 30.978 184 113 7 7 184 4 179 5.29e-15 77.0
MS.gene59169.t1 AT5G16410 24.378 402 229 14 62 402 89 476 7.17e-14 73.6
MS.gene59169.t1 AT5G07870 27.799 259 162 11 64 302 72 325 9.57e-14 73.2
MS.gene59169.t1 AT5G47950 24.306 288 174 10 7 281 4 260 1.82e-13 72.0
MS.gene59169.t1 AT3G23840 28.293 205 126 8 73 269 72 263 1.67e-11 66.2
MS.gene59169.t1 AT4G15400 23.282 451 261 18 15 406 11 435 2.67e-11 65.5

Find 107 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCCATTGCTGCTTATTC+AGG 0.196232 1.4:-24812125 MS.gene59169:intron
CAACATTAACCAACCTACTA+TGG 0.247529 1.4:-24810031 MS.gene59169:CDS
TAAAGGGATAATTGTTTCTT+TGG 0.270402 1.4:+24810566 None:intergenic
TCATCCTCCATTTCCTTGCT+TGG 0.281673 1.4:-24812191 MS.gene59169:CDS
GAAATAATGAAAATAGGTTT+TGG 0.315255 1.4:+24810003 None:intergenic
AAGGAAGAAATAATGAAAAT+AGG 0.323796 1.4:+24809997 None:intergenic
ATTCTCTGCTCTTTCTTCTT+CGG 0.349771 1.4:-24810534 MS.gene59169:CDS
TCACTTTGAATACTAGAGAT+TGG 0.355410 1.4:+24812536 None:intergenic
GATGTTACTCTTATACAATT+TGG 0.359716 1.4:-24812223 MS.gene59169:CDS
AAATCCTCACTGCATTCCTT+TGG 0.393738 1.4:-24810446 MS.gene59169:CDS
CACTTTGAATACTAGAGATT+GGG 0.397967 1.4:+24812537 None:intergenic
AGCTGATCCAAGCAAGGAAA+TGG 0.402058 1.4:+24812184 None:intergenic
AGGTCCTGGCAGGAAACTTA+TGG 0.416255 1.4:-24812290 MS.gene59169:CDS
GGTTGGGGAAAACCTTGTTA+TGG 0.420339 1.4:-24810198 MS.gene59169:CDS
CAGCTTCTATACGATGTTCC+TGG 0.423861 1.4:-24812166 MS.gene59169:CDS
CGATGAGTGCATGTTACTCT+TGG 0.426648 1.4:+24810612 None:intergenic
TGCTAAAGGAGTAGAAGGTT+TGG 0.428539 1.4:-24810124 MS.gene59169:CDS
GATGAGTGATGCTGCTGGTT+TGG 0.433088 1.4:-24810733 MS.gene59169:CDS
CGAACTTCTTCATCGATGTC+TGG 0.436986 1.4:+24810399 None:intergenic
AACTGGATCTTTCCCTGCCA+TGG 0.438203 1.4:+24812381 None:intergenic
ATAATTGTTTCTTTGGTGTC+GGG 0.439179 1.4:+24810573 None:intergenic
AGAGAGTTAGATTTCGGTTG+GGG 0.441093 1.4:-24810213 MS.gene59169:CDS
GGATCTTTCCCTGCCATGGT+TGG 0.441588 1.4:+24812385 None:intergenic
CGCCTAAGGCACTCATGAAT+CGG 0.443530 1.4:+24810709 None:intergenic
AACCTCTCCATGGCTTTAGT+TGG 0.444544 1.4:+24810084 None:intergenic
ACCGTAGTAACCGTTTGGTA+AGG 0.450110 1.4:+24810341 None:intergenic
CAAAATTAGAGTGTTGTTGT+TGG 0.454490 1.4:+24810469 None:intergenic
TAGAGAGTTAGATTTCGGTT+GGG 0.455559 1.4:-24810214 MS.gene59169:CDS
GCTTTAGTTGGCAAACAAAC+CGG 0.457440 1.4:+24810096 None:intergenic
GTCCGATTCATGAGTGCCTT+AGG 0.459205 1.4:-24810711 MS.gene59169:CDS
CCATCTTGTTCATCCCTGTC+TGG 0.459657 1.4:+24812451 None:intergenic
ATCCATTTGCAGGTAGATTG+AGG 0.464119 1.4:-24812315 MS.gene59169:CDS
CAGAGAATTGTGGCATGTAA+AGG 0.466829 1.4:+24810549 None:intergenic
GATAATTGTTTCTTTGGTGT+CGG 0.469946 1.4:+24810572 None:intergenic
TCCCTCAATCTACCTGCAAA+TGG 0.471845 1.4:+24812313 None:intergenic
AACTACGATCCAACCATGGC+AGG 0.475216 1.4:-24812394 MS.gene59169:CDS
AGTAGAAGGTTTGGTTATAC+CGG 0.475564 1.4:-24810115 MS.gene59169:CDS
TTAGAGAGTTAGATTTCGGT+TGG 0.481429 1.4:-24810215 MS.gene59169:CDS
AAAGAAAATTACTTCCAGAC+AGG 0.481528 1.4:-24812465 MS.gene59169:CDS
TCCATTTGCAGGTAGATTGA+GGG 0.491578 1.4:-24812314 MS.gene59169:CDS
TTGCTCTTCGTCTAAACCAT+AGG 0.494992 1.4:-24810755 MS.gene59169:CDS
AGAGAATTGTGGCATGTAAA+GGG 0.496978 1.4:+24810550 None:intergenic
GGAGAGGTTCATTAAAGAGT+TGG 0.500199 1.4:-24810070 MS.gene59169:CDS
AAACTTATGGTTGATTGTAA+TGG 0.500661 1.4:-24812277 MS.gene59169:CDS
GTGGTTGATGTTAAACATGC+TGG 0.504572 1.4:-24810240 MS.gene59169:CDS
TGCCATGCTGGTGAGATTGA+AGG 0.507840 1.4:+24810663 None:intergenic
GGGATGAACAAGATGGATTA+CGG 0.508792 1.4:-24812444 MS.gene59169:CDS
TGAGTGCATGTTACTCTTGG+AGG 0.513001 1.4:+24810615 None:intergenic
CAGCAGCATCACTCATCCTA+TGG 0.516343 1.4:+24810739 None:intergenic
GTGTTACCGTAGTAACCGTT+TGG 0.518079 1.4:+24810336 None:intergenic
CCCTTACCAAACGGTTACTA+CGG 0.520851 1.4:-24810342 MS.gene59169:CDS
CCCATGAGATATTTCGCCTA+AGG 0.523720 1.4:+24810695 None:intergenic
ATTAACCCACCCTTACCAAA+CGG 0.526261 1.4:-24810351 MS.gene59169:CDS
TTGTTTGCCAACTAAAGCCA+TGG 0.530156 1.4:-24810091 MS.gene59169:CDS
CCGTAGTAACCGTTTGGTAA+GGG 0.532489 1.4:+24810342 None:intergenic
GCCTTAGGCGAAATATCTCA+TGG 0.541451 1.4:-24810696 MS.gene59169:CDS
GAAGAAAGAGCAGAGAATTG+TGG 0.542995 1.4:+24810539 None:intergenic
TAGTTGTAAAATTGTATCAC+AGG 0.546959 1.4:+24812412 None:intergenic
GAGTGTTGTTGTTGGTGAGG+TGG 0.551701 1.4:+24810477 None:intergenic
GATTGAGGGAAGGTCCTGGC+AGG 0.552476 1.4:-24812300 MS.gene59169:CDS
AGAAGCTCTCTATGCCATGC+TGG 0.552535 1.4:+24810651 None:intergenic
AACATTAACCAACCTACTAT+GGG 0.554023 1.4:-24810030 MS.gene59169:CDS
ATAGAGAGCTTCTAAACGCA+AGG 0.554177 1.4:-24810641 MS.gene59169:CDS
TATACCTGAATAAGCAGCAA+TGG 0.559109 1.4:+24812121 None:intergenic
CTCTTTAATGAACCTCTCCA+TGG 0.561562 1.4:+24810074 None:intergenic
TAGTAACCGTTTGGTAAGGG+TGG 0.562247 1.4:+24810345 None:intergenic
GAAACAACTCCTCCTTTAGC+TGG 0.563051 1.4:+24810171 None:intergenic
TGTTATGGAGGACCAGCTAA+AGG 0.564399 1.4:-24810183 MS.gene59169:CDS
ACTGCAGTTGTAGCCGCGGA+AGG 0.565373 1.4:+24810303 None:intergenic
ACATTAGTTCAAGGTCTACA+AGG 0.565448 1.4:+24809978 None:intergenic
GGTAGATTGAGGGAAGGTCC+TGG 0.568132 1.4:-24812304 MS.gene59169:CDS
CTCTAGTATTCAAAGTGAAG+CGG 0.576350 1.4:-24812531 MS.gene59169:CDS
CAATTGTACGACAACGCCAA+AGG 0.577956 1.4:+24810430 None:intergenic
GCGAATTGCAGCTACCTCAG+TGG 0.579537 1.4:+24810509 None:intergenic
TTAGAGTGTTGTTGTTGGTG+AGG 0.580129 1.4:+24810474 None:intergenic
TTGGACCACCCATAGTAGGT+TGG 0.580335 1.4:+24810022 None:intergenic
TTAGCTGGTCCTCCATAACA+AGG 0.580970 1.4:+24810186 None:intergenic
ACTACGATCCAACCATGGCA+GGG 0.581910 1.4:-24812393 MS.gene59169:CDS
CGAATTGCAGCTACCTCAGT+GGG 0.590147 1.4:+24810510 None:intergenic
CTTTACTGCAGTTGTAGCCG+CGG 0.598947 1.4:+24810299 None:intergenic
AGTAACCGTTTGGTAAGGGT+GGG 0.602355 1.4:+24810346 None:intergenic
CTAAGCATATCTGAGGAACC+AGG 0.605844 1.4:+24812148 None:intergenic
TGATCCAAGCAAGGAAATGG+AGG 0.612576 1.4:+24812187 None:intergenic
TCAACCATAAGTTTCCTGCC+AGG 0.615495 1.4:+24812286 None:intergenic
TGGGGAAAACCTTGTTATGG+AGG 0.615938 1.4:-24810195 MS.gene59169:CDS
TATAGAAGCTGATCCAAGCA+AGG 0.620895 1.4:+24812178 None:intergenic
GCTCTCACCACAGTAAAATG+AGG 0.627802 1.4:+24810270 None:intergenic
ATGGTTGATTGTAATGGTGA+AGG 0.627996 1.4:-24812271 MS.gene59169:CDS
AAGAAAATTACTTCCAGACA+GGG 0.632501 1.4:-24812464 MS.gene59169:CDS
TGCCAACTAAAGCCATGGAG+AGG 0.635260 1.4:-24810086 MS.gene59169:CDS
TCACCACAGTAAAATGAGGT+CGG 0.638789 1.4:+24810274 None:intergenic
CCAGACAGGGATGAACAAGA+TGG 0.641844 1.4:-24812451 MS.gene59169:CDS
CCTTAGGCGAAATATCTCAT+GGG 0.643188 1.4:-24810695 MS.gene59169:CDS
GTAACACGTCTTAAGTGCGG+TGG 0.646528 1.4:-24810786 MS.gene59169:CDS
TGGAGTGCTAAGCATATCTG+AGG 0.646945 1.4:+24812141 None:intergenic
ATTAACCAACCTACTATGGG+TGG 0.647419 1.4:-24810027 MS.gene59169:CDS
GTTCGCATAGTCAATGCGCA+TGG 0.650306 1.4:-24810381 MS.gene59169:CDS
AACCTTCAATCTCACCAGCA+TGG 0.650946 1.4:-24810665 MS.gene59169:CDS
TAGAGAGCTTCTAAACGCAA+GGG 0.655440 1.4:-24810640 MS.gene59169:CDS
TTTGCAGGTAGATTGAGGGA+AGG 0.669320 1.4:-24812310 MS.gene59169:CDS
CATAGGATGAGTGATGCTGC+TGG 0.673345 1.4:-24810738 MS.gene59169:CDS
TTACAACTACGATCCAACCA+TGG 0.676438 1.4:-24812398 MS.gene59169:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.679087 1.4:-24810180 MS.gene59169:CDS
CAGGTAACACGTCTTAAGTG+CGG 0.702810 1.4:-24810789 MS.gene59169:CDS
AACGCCAAAGGAATGCAGTG+AGG 0.720001 1.4:+24810442 None:intergenic
TTTCTTCTTCGGTCCCACTG+AGG 0.728927 1.4:-24810523 MS.gene59169:CDS
TGTGGTGAGAGCATATCTAG+TGG 0.753755 1.4:-24810259 MS.gene59169:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTATTTATTTATACAAAAT+AGG + chr1.4:24811168-24811187 None:intergenic 10.0%
!!! ATTTTCTTTTCAAAATTATT+GGG + chr1.4:24810585-24810604 None:intergenic 10.0%
!!! TCTCTTATTTTTTATTTTTT+TGG + chr1.4:24811200-24811219 None:intergenic 10.0%
!!! TTGCATTTATTTTAAAAAAT+AGG - chr1.4:24811301-24811320 MS.gene59169:intron 10.0%
!! TTAGTGTTAAATTAAAAGTA+GGG - chr1.4:24811013-24811032 MS.gene59169:intron 15.0%
!! TTTAGTGTTAAATTAAAAGT+AGG - chr1.4:24811012-24811031 MS.gene59169:intron 15.0%
!!! ATAATTTTGAAAAGAAAATG+AGG - chr1.4:24810586-24810605 MS.gene59169:CDS 15.0%
!!! CATTTTCTTTTCAAAATTAT+TGG + chr1.4:24810586-24810605 None:intergenic 15.0%
!!! TTTTGTAGTTTTTTTTCTTA+GGG - chr1.4:24811562-24811581 MS.gene59169:intron 15.0%
!! AAGGAAGAAATAATGAAAAT+AGG + chr1.4:24812540-24812559 None:intergenic 20.0%
!! ATAGATAGTGTTTATAAGTT+GGG + chr1.4:24811081-24811100 None:intergenic 20.0%
!! CTAGGTTATATATTTATGTT+TGG + chr1.4:24811267-24811286 None:intergenic 20.0%
!! TAAAAAACAGTTAAATTGCA+GGG + chr1.4:24810918-24810937 None:intergenic 20.0%
!! TATAGATAGTGTTTATAAGT+TGG + chr1.4:24811082-24811101 None:intergenic 20.0%
!! TCAATTCAATTATTTCATGT+TGG - chr1.4:24810973-24810992 MS.gene59169:intron 20.0%
!!! AACACATGTGATTATTTTTT+AGG + chr1.4:24811638-24811657 None:intergenic 20.0%
!!! ATACCTTTTAAAAATGCTAA+AGG - chr1.4:24812396-24812415 MS.gene59169:CDS 20.0%
!!! ATTAGATTTTACAAGCAATA+AGG + chr1.4:24810661-24810680 None:intergenic 20.0%
!!! ATTTATTTAAAATTGCCCTT+GGG + chr1.4:24811436-24811455 None:intergenic 20.0%
!!! ATTTTTCGAATATATGTATG+AGG + chr1.4:24811235-24811254 None:intergenic 20.0%
!!! GAAATAATGAAAATAGGTTT+TGG + chr1.4:24812534-24812553 None:intergenic 20.0%
!!! GTTTTGTAGTTTTTTTTCTT+AGG - chr1.4:24811561-24811580 MS.gene59169:intron 20.0%
!!! TAGCATTTTTTTTTGTTGAA+CGG + chr1.4:24811125-24811144 None:intergenic 20.0%
!!! TATTTATTTAAAATTGCCCT+TGG + chr1.4:24811437-24811456 None:intergenic 20.0%
!!! TGAAATATTTTAACGTGTTT+TGG + chr1.4:24810827-24810846 None:intergenic 20.0%
!!! TTTGTAGTTTTTTTTCTTAG+GGG - chr1.4:24811563-24811582 MS.gene59169:intron 20.0%
! AAACTTATGGTTGATTGTAA+TGG - chr1.4:24810257-24810276 MS.gene59169:CDS 25.0%
! AAAGTAAGGTTGAATATATG+AGG - chr1.4:24810612-24810631 MS.gene59169:CDS 25.0%
! CTAAAAAACAGTTAAATTGC+AGG + chr1.4:24810919-24810938 None:intergenic 25.0%
! GAAATAATTGAATTGAACCA+CGG + chr1.4:24810969-24810988 None:intergenic 25.0%
! GATGTTACTCTTATACAATT+TGG - chr1.4:24810311-24810330 MS.gene59169:CDS 25.0%
! TAGTTGTAAAATTGTATCAC+AGG + chr1.4:24810125-24810144 None:intergenic 25.0%
! TATTTCATGTTGGATAAGTT+TGG - chr1.4:24810983-24811002 MS.gene59169:intron 25.0%
! TGTTAAATTAAAAGTAGGGT+TGG - chr1.4:24811017-24811036 MS.gene59169:intron 25.0%
!! ACTCCTTTAGCATTTTTAAA+AGG + chr1.4:24812402-24812421 None:intergenic 25.0%
!! CAGATTTTCGAATCTAAATA+GGG - chr1.4:24810845-24810864 MS.gene59169:intron 25.0%
!! GCTTTTCTAATAATATCAAC+TGG + chr1.4:24810173-24810192 None:intergenic 25.0%
!! TAAAGGGATAATTGTTTCTT+TGG + chr1.4:24811971-24811990 None:intergenic 25.0%
!! TCAGATTTTCGAATCTAAAT+AGG - chr1.4:24810844-24810863 MS.gene59169:intron 25.0%
!! TTAGTCTTCATAATATGCTT+CGG - chr1.4:24810442-24810461 MS.gene59169:CDS 25.0%
!! TTTATTTAAAATTGCCCTTG+GGG + chr1.4:24811435-24811454 None:intergenic 25.0%
!!! AAATTGCAGGGTTTAATTTT+AGG + chr1.4:24810906-24810925 None:intergenic 25.0%
!!! AATATTTTAACGTGTTTTGG+AGG + chr1.4:24810824-24810843 None:intergenic 25.0%
!!! GGTTTTGTAGTTTTTTCTTT+GGG + chr1.4:24811377-24811396 None:intergenic 25.0%
!!! GTTTTGTAGTTTTTTCTTTG+GGG + chr1.4:24811376-24811395 None:intergenic 25.0%
!!! GTTTTTTAGAGTTTTCTCTT+TGG - chr1.4:24810930-24810949 MS.gene59169:intron 25.0%
!!! TAATCACATGTGTTTGATTT+TGG - chr1.4:24811644-24811663 MS.gene59169:intron 25.0%
!!! TTGATGGTTTTACAAATTCA+AGG + chr1.4:24810034-24810053 None:intergenic 25.0%
!!! TTGTAGTTTTTTTTCTTAGG+GGG - chr1.4:24811564-24811583 MS.gene59169:intron 25.0%
!!! TTTTGTAGTTTTTTCTTTGG+GGG + chr1.4:24811375-24811394 None:intergenic 25.0%
AAAAATGCTAAAGGAGTAGA+AGG - chr1.4:24812405-24812424 MS.gene59169:CDS 30.0%
AAAGAAAATTACTTCCAGAC+AGG - chr1.4:24810069-24810088 MS.gene59169:CDS 30.0%
AACATTAACCAACCTACTAT+GGG - chr1.4:24812504-24812523 MS.gene59169:CDS 30.0%
AAGAAAATTACTTCCAGACA+GGG - chr1.4:24810070-24810089 MS.gene59169:CDS 30.0%
AATAAGGCCTCATATTCTAA+GGG + chr1.4:24810645-24810664 None:intergenic 30.0%
AGAAAATGAGGTCAAAAGTA+AGG - chr1.4:24810598-24810617 MS.gene59169:CDS 30.0%
CACTTTGAATACTAGAGATT+GGG + chr1.4:24810000-24810019 None:intergenic 30.0%
GATTAGATTCACAGATATCT+TGG + chr1.4:24810499-24810518 None:intergenic 30.0%
TCACTTTGAATACTAGAGAT+TGG + chr1.4:24810001-24810020 None:intergenic 30.0%
TTATATAATCCGAACTGCAT+AGG + chr1.4:24810773-24810792 None:intergenic 30.0%
TTTCATGCTTTATGCTTTGA+TGG - chr1.4:24810693-24810712 MS.gene59169:CDS 30.0%
TTTCGAATATATGTATGAGG+CGG + chr1.4:24811232-24811251 None:intergenic 30.0%
! AATTTTCTTTCATGAGGTGT+TGG + chr1.4:24810059-24810078 None:intergenic 30.0%
! ATAGTGTTTATAAGTTGGGT+TGG + chr1.4:24811077-24811096 None:intergenic 30.0%
! GATAATTGTTTCTTTGGTGT+CGG + chr1.4:24811965-24811984 None:intergenic 30.0%
! GGAAGTAATTTTCTTTCATG+AGG + chr1.4:24810065-24810084 None:intergenic 30.0%
! TAGTGTTTATAAGTTGGGTT+GGG + chr1.4:24811076-24811095 None:intergenic 30.0%
! TTATTTAAAATTGCCCTTGG+GGG + chr1.4:24811434-24811453 None:intergenic 30.0%
!! ATAATTGTTTCTTTGGTGTC+GGG + chr1.4:24811964-24811983 None:intergenic 30.0%
!! CAAAATTAGAGTGTTGTTGT+TGG + chr1.4:24812068-24812087 None:intergenic 30.0%
!! CGGTTTTGTAGTTTTTTCTT+TGG + chr1.4:24811378-24811397 None:intergenic 30.0%
!!! ACCAAGTCAAAATTGGTTTT+GGG - chr1.4:24811503-24811522 MS.gene59169:intron 30.0%
!!! CTTTTTTCCCTTAGAATATG+AGG - chr1.4:24810635-24810654 MS.gene59169:CDS 30.0%
!!! GGTTTTAGAGAGTTAGATTT+CGG - chr1.4:24812315-24812334 MS.gene59169:CDS 30.0%
AAGCAAAAGCTTCAAGGTTA+AGG + chr1.4:24810719-24810738 None:intergenic 35.0%
AGAGAATTGTGGCATGTAAA+GGG + chr1.4:24811987-24812006 None:intergenic 35.0%
ATCGCAAATTCGTGAAAGTT+AGG + chr1.4:24811347-24811366 None:intergenic 35.0%
ATGGTTGATTGTAATGGTGA+AGG - chr1.4:24810263-24810282 MS.gene59169:CDS 35.0%
ATTCTCTGCTCTTTCTTCTT+CGG - chr1.4:24812000-24812019 MS.gene59169:intron 35.0%
CAACATTAACCAACCTACTA+TGG - chr1.4:24812503-24812522 MS.gene59169:CDS 35.0%
CAATAAGGCCTCATATTCTA+AGG + chr1.4:24810646-24810665 None:intergenic 35.0%
CTCTAGTATTCAAAGTGAAG+CGG - chr1.4:24810003-24810022 MS.gene59169:CDS 35.0%
GCAAGTTCAATACATTAGCT+AGG + chr1.4:24811285-24811304 None:intergenic 35.0%
TAGAGAGTTAGATTTCGGTT+GGG - chr1.4:24812320-24812339 MS.gene59169:CDS 35.0%
TATACCTGAATAAGCAGCAA+TGG + chr1.4:24810416-24810435 None:intergenic 35.0%
TCGCAAATTCGTGAAAGTTA+GGG + chr1.4:24811346-24811365 None:intergenic 35.0%
TCTTCATAATATGCTTCGGT+TGG - chr1.4:24810446-24810465 MS.gene59169:CDS 35.0%
TTAGAGAGTTAGATTTCGGT+TGG - chr1.4:24812319-24812338 MS.gene59169:CDS 35.0%
TTCGAATATATGTATGAGGC+GGG + chr1.4:24811231-24811250 None:intergenic 35.0%
! AGTAGAAGGTTTGGTTATAC+CGG - chr1.4:24812419-24812438 MS.gene59169:CDS 35.0%
! ATAATATGCTTCGGTTGGTT+TGG - chr1.4:24810451-24810470 MS.gene59169:CDS 35.0%
! ATCGCAACTTTGAGAAAGTT+TGG - chr1.4:24811592-24811611 MS.gene59169:intron 35.0%
! CTTTCACGAATTTGCGATTT+TGG - chr1.4:24811349-24811368 MS.gene59169:intron 35.0%
! CTTTCTCAAAGTTGCGATTT+TGG + chr1.4:24811590-24811609 None:intergenic 35.0%
! GTGTTTTATCATCCATTTGC+AGG - chr1.4:24810209-24810228 MS.gene59169:CDS 35.0%
! TATTTAAAATTGCCCTTGGG+GGG + chr1.4:24811433-24811452 None:intergenic 35.0%
! TCGCAACTTTGAGAAAGTTT+GGG - chr1.4:24811593-24811612 MS.gene59169:intron 35.0%
!! GACCAAGTCAAAATTGGTTT+TGG - chr1.4:24811502-24811521 MS.gene59169:intron 35.0%
!! TAATATGCTTCGGTTGGTTT+GGG - chr1.4:24810452-24810471 MS.gene59169:CDS 35.0%
!!! ACGTGTTTTGGAGGTTTTTA+TGG + chr1.4:24810815-24810834 None:intergenic 35.0%
!!! CCAAGTCAAAATTGGTTTTG+GGG - chr1.4:24811504-24811523 MS.gene59169:intron 35.0%
!!! CGTGTTTTGGAGGTTTTTAT+GGG + chr1.4:24810814-24810833 None:intergenic 35.0%
!!! GTGTTTTGGAGGTTTTTATG+GGG + chr1.4:24810813-24810832 None:intergenic 35.0%
!!! TTGGCATATTTTTAGAACCG+TGG - chr1.4:24810949-24810968 MS.gene59169:intron 35.0%
AAATATGTGGCACCTCTCTT+AGG - chr1.4:24811463-24811482 MS.gene59169:intron 40.0%
AAATCCTCACTGCATTCCTT+TGG - chr1.4:24812088-24812107 MS.gene59169:intron 40.0%
AATATGTGGCACCTCTCTTA+GGG - chr1.4:24811464-24811483 MS.gene59169:intron 40.0%
ACACTATCACACAAATCCCA+GGG - chr1.4:24810556-24810575 MS.gene59169:CDS 40.0%
AGAGAGTTAGATTTCGGTTG+GGG - chr1.4:24812321-24812340 MS.gene59169:CDS 40.0%
AGCGTAGACCAAGTCAAAAT+TGG - chr1.4:24811496-24811515 MS.gene59169:intron 40.0%
AGGACATGCGAGAAATCAAT+TGG + chr1.4:24810753-24810772 None:intergenic 40.0%
AGGGTGCACGAGAAATTAAT+AGG - chr1.4:24810864-24810883 MS.gene59169:intron 40.0%
ATAGAGAGCTTCTAAACGCA+AGG - chr1.4:24811893-24811912 MS.gene59169:intron 40.0%
ATCCATTTGCAGGTAGATTG+AGG - chr1.4:24810219-24810238 MS.gene59169:CDS 40.0%
ATGCGAGAAATCAATTGGGT+GGG + chr1.4:24810748-24810767 None:intergenic 40.0%
ATTAACCAACCTACTATGGG+TGG - chr1.4:24812507-24812526 MS.gene59169:CDS 40.0%
ATTAACCCACCCTTACCAAA+CGG - chr1.4:24812183-24812202 MS.gene59169:CDS 40.0%
CAGAGAATTGTGGCATGTAA+AGG + chr1.4:24811988-24812007 None:intergenic 40.0%
CCTTAGGCGAAATATCTCAT+GGG - chr1.4:24811839-24811858 MS.gene59169:intron 40.0%
CGCAAATTCGTGAAAGTTAG+GGG + chr1.4:24811345-24811364 None:intergenic 40.0%
CGGATGTGTCTATAAGTCAA+CGG + chr1.4:24810521-24810540 None:intergenic 40.0%
CTCTTTAATGAACCTCTCCA+TGG + chr1.4:24812463-24812482 None:intergenic 40.0%
CTTGAAGTGAAATTGTGTGC+AGG - chr1.4:24811726-24811745 MS.gene59169:intron 40.0%
GAAGAAAGAGCAGAGAATTG+TGG + chr1.4:24811998-24812017 None:intergenic 40.0%
GCAAATTCGTGAAAGTTAGG+GGG + chr1.4:24811344-24811363 None:intergenic 40.0%
GCTTTAGTTGGCAAACAAAC+CGG + chr1.4:24812441-24812460 None:intergenic 40.0%
GGACATGCGAGAAATCAATT+GGG + chr1.4:24810752-24810771 None:intergenic 40.0%
GGAGAGGTTCATTAAAGAGT+TGG - chr1.4:24812464-24812483 MS.gene59169:CDS 40.0%
TAGAGAGCTTCTAAACGCAA+GGG - chr1.4:24811894-24811913 MS.gene59169:intron 40.0%
TATAGAAGCTGATCCAAGCA+AGG + chr1.4:24810359-24810378 None:intergenic 40.0%
TCACCACAGTAAAATGAGGT+CGG + chr1.4:24812263-24812282 None:intergenic 40.0%
TCCATTTGCAGGTAGATTGA+GGG - chr1.4:24810220-24810239 MS.gene59169:CDS 40.0%
TGCCACAGTTGCAAAACTTA+GGG + chr1.4:24811403-24811422 None:intergenic 40.0%
TGCTAAAGGAGTAGAAGGTT+TGG - chr1.4:24812410-24812429 MS.gene59169:CDS 40.0%
TTACAACTACGATCCAACCA+TGG - chr1.4:24810136-24810155 MS.gene59169:CDS 40.0%
TTGCTCTTCGTCTAAACCAT+AGG - chr1.4:24811779-24811798 MS.gene59169:intron 40.0%
TTGTTTGCCAACTAAAGCCA+TGG - chr1.4:24812443-24812462 MS.gene59169:CDS 40.0%
! CCTTGAAGCTTTTGCTTTGA+CGG - chr1.4:24810722-24810741 MS.gene59169:CDS 40.0%
! CGCAACTTTGAGAAAGTTTG+GGG - chr1.4:24811594-24811613 MS.gene59169:intron 40.0%
! GCAACTTTGAGAAAGTTTGG+GGG - chr1.4:24811595-24811614 MS.gene59169:intron 40.0%
! GTGGTTGATGTTAAACATGC+TGG - chr1.4:24812294-24812313 MS.gene59169:CDS 40.0%
! TTTTGCAACTGTGGCAGTTT+TGG - chr1.4:24811407-24811426 MS.gene59169:intron 40.0%
!! GGGATGAACAAGATGGATTA+CGG - chr1.4:24810090-24810109 MS.gene59169:CDS 40.0%
!! TTAGAGTGTTGTTGTTGGTG+AGG + chr1.4:24812063-24812082 None:intergenic 40.0%
!!! CCCCAAAACCAATTTTGACT+TGG + chr1.4:24811507-24811526 None:intergenic 40.0%
!!! TCATGAGGTGTTGGTTTTGA+TGG + chr1.4:24810050-24810069 None:intergenic 40.0%
AACCTTCAATCTCACCAGCA+TGG - chr1.4:24811869-24811888 MS.gene59169:intron 45.0%
ACCGTAGTAACCGTTTGGTA+AGG + chr1.4:24812196-24812215 None:intergenic 45.0%
AGCTGATCCAAGCAAGGAAA+TGG + chr1.4:24810353-24810372 None:intergenic 45.0%
AGTAACCGTTTGGTAAGGGT+GGG + chr1.4:24812191-24812210 None:intergenic 45.0%
CAATTGTACGACAACGCCAA+AGG + chr1.4:24812107-24812126 None:intergenic 45.0%
CACACTATCACACAAATCCC+AGG - chr1.4:24810555-24810574 MS.gene59169:CDS 45.0%
CAGCTTCTATACGATGTTCC+TGG - chr1.4:24810368-24810387 MS.gene59169:CDS 45.0%
CAGGTAACACGTCTTAAGTG+CGG - chr1.4:24811745-24811764 MS.gene59169:intron 45.0%
CATGCGAGAAATCAATTGGG+TGG + chr1.4:24810749-24810768 None:intergenic 45.0%
CCACAGTTGCAAAACTTAGG+GGG + chr1.4:24811401-24811420 None:intergenic 45.0%
CCCATGAGATATTTCGCCTA+AGG + chr1.4:24811842-24811861 None:intergenic 45.0%
CCCTTACCAAACGGTTACTA+CGG - chr1.4:24812192-24812211 MS.gene59169:CDS 45.0%
CCGTAGTAACCGTTTGGTAA+GGG + chr1.4:24812195-24812214 None:intergenic 45.0%
CCGTCAAAGCAAAAGCTTCA+AGG + chr1.4:24810725-24810744 None:intergenic 45.0%
CGAACTTCTTCATCGATGTC+TGG + chr1.4:24812138-24812157 None:intergenic 45.0%
CGATGAGTGCATGTTACTCT+TGG + chr1.4:24811925-24811944 None:intergenic 45.0%
CTAAGCATATCTGAGGAACC+AGG + chr1.4:24810389-24810408 None:intergenic 45.0%
CTGCCACAGTTGCAAAACTT+AGG + chr1.4:24811404-24811423 None:intergenic 45.0%
GAAACAACTCCTCCTTTAGC+TGG + chr1.4:24812366-24812385 None:intergenic 45.0%
GCCACAGTTGCAAAACTTAG+GGG + chr1.4:24811402-24811421 None:intergenic 45.0%
GCCTTAGGCGAAATATCTCA+TGG - chr1.4:24811838-24811857 MS.gene59169:intron 45.0%
GCTCTCACCACAGTAAAATG+AGG + chr1.4:24812267-24812286 None:intergenic 45.0%
GGTTGGGGAAAACCTTGTTA+TGG - chr1.4:24812336-24812355 MS.gene59169:CDS 45.0%
GTGTTACCGTAGTAACCGTT+TGG + chr1.4:24812201-24812220 None:intergenic 45.0%
TAGTAACCGTTTGGTAAGGG+TGG + chr1.4:24812192-24812211 None:intergenic 45.0%
TCAACCATAAGTTTCCTGCC+AGG + chr1.4:24810251-24810270 None:intergenic 45.0%
TCATCCTCCATTTCCTTGCT+TGG - chr1.4:24810343-24810362 MS.gene59169:CDS 45.0%
TCCCTCAATCTACCTGCAAA+TGG + chr1.4:24810224-24810243 None:intergenic 45.0%
TGAGTGCATGTTACTCTTGG+AGG + chr1.4:24811922-24811941 None:intergenic 45.0%
TGATCCAAGCAAGGAAATGG+AGG + chr1.4:24810350-24810369 None:intergenic 45.0%
TGCCACACGTGCAAAACTTA+GGG - chr1.4:24811534-24811553 MS.gene59169:intron 45.0%
TGTTATGGAGGACCAGCTAA+AGG - chr1.4:24812351-24812370 MS.gene59169:CDS 45.0%
TTAGCTGGTCCTCCATAACA+AGG + chr1.4:24812351-24812370 None:intergenic 45.0%
TTTGCAGGTAGATTGAGGGA+AGG - chr1.4:24810224-24810243 MS.gene59169:CDS 45.0%
! CACTCCATTGCTGCTTATTC+AGG - chr1.4:24810409-24810428 MS.gene59169:CDS 45.0%
! CACTTTTGCATTTCCTTCCG+CGG - chr1.4:24812218-24812237 MS.gene59169:CDS 45.0%
! TGGAGTGCTAAGCATATCTG+AGG + chr1.4:24810396-24810415 None:intergenic 45.0%
! TGGGGAAAACCTTGTTATGG+AGG - chr1.4:24812339-24812358 MS.gene59169:CDS 45.0%
! TGTGGTGAGAGCATATCTAG+TGG - chr1.4:24812275-24812294 MS.gene59169:CDS 45.0%
! TTTTGCACGTGTGGCAGTTT+TGG + chr1.4:24811530-24811549 None:intergenic 45.0%
!! AACCTCTCCATGGCTTTAGT+TGG + chr1.4:24812453-24812472 None:intergenic 45.0%
!! CTTCGGTTGGTTTGGGTATT+GGG - chr1.4:24810459-24810478 MS.gene59169:CDS 45.0%
!! ATAAATAAAAAAAAAATATG+TGG - chr1.4:24811450-24811469 MS.gene59169:intron 5.0%
AACGCCAAAGGAATGCAGTG+AGG + chr1.4:24812095-24812114 None:intergenic 50.0%
AACTACGATCCAACCATGGC+AGG - chr1.4:24810140-24810159 MS.gene59169:CDS 50.0%
AACTGGATCTTTCCCTGCCA+TGG + chr1.4:24810156-24810175 None:intergenic 50.0%
ACTACGATCCAACCATGGCA+GGG - chr1.4:24810141-24810160 MS.gene59169:CDS 50.0%
AGAAGCTCTCTATGCCATGC+TGG + chr1.4:24811886-24811905 None:intergenic 50.0%
AGGTCCTGGCAGGAAACTTA+TGG - chr1.4:24810244-24810263 MS.gene59169:CDS 50.0%
AGTGTGTTTGTGCGCATGTG+CGG + chr1.4:24810541-24810560 None:intergenic 50.0%
CAAAATTATTGGGCCGCCCT+GGG + chr1.4:24810575-24810594 None:intergenic 50.0%
CAGCAGCATCACTCATCCTA+TGG + chr1.4:24811798-24811817 None:intergenic 50.0%
CAGTTGCAAAACTTAGGGGG+CGG + chr1.4:24811398-24811417 None:intergenic 50.0%
CATAGGATGAGTGATGCTGC+TGG - chr1.4:24811796-24811815 MS.gene59169:intron 50.0%
CCACACGTGCAAAACTTAGG+GGG - chr1.4:24811536-24811555 MS.gene59169:intron 50.0%
CCAGACAGGGATGAACAAGA+TGG - chr1.4:24810083-24810102 MS.gene59169:CDS 50.0%
CCATCTTGTTCATCCCTGTC+TGG + chr1.4:24810086-24810105 None:intergenic 50.0%
CGAATTGCAGCTACCTCAGT+GGG + chr1.4:24812027-24812046 None:intergenic 50.0%
CTATCACACAAATCCCAGGG+CGG - chr1.4:24810559-24810578 MS.gene59169:CDS 50.0%
CTCGCATGTCCTATGCAGTT+CGG - chr1.4:24810761-24810780 MS.gene59169:CDS 50.0%
CTGCCACACGTGCAAAACTT+AGG - chr1.4:24811533-24811552 MS.gene59169:intron 50.0%
CTTTACTGCAGTTGTAGCCG+CGG + chr1.4:24812238-24812257 None:intergenic 50.0%
GCCACACGTGCAAAACTTAG+GGG - chr1.4:24811535-24811554 MS.gene59169:intron 50.0%
GTAACACGTCTTAAGTGCGG+TGG - chr1.4:24811748-24811767 MS.gene59169:intron 50.0%
GTCCGATTCATGAGTGCCTT+AGG - chr1.4:24811823-24811842 MS.gene59169:intron 50.0%
GTTCGCATAGTCAATGCGCA+TGG - chr1.4:24812153-24812172 MS.gene59169:CDS 50.0%
TATGGAGGACCAGCTAAAGG+AGG - chr1.4:24812354-24812373 MS.gene59169:CDS 50.0%
TCAAAATTATTGGGCCGCCC+TGG + chr1.4:24810576-24810595 None:intergenic 50.0%
TGCCAACTAAAGCCATGGAG+AGG - chr1.4:24812448-24812467 MS.gene59169:CDS 50.0%
TGCCATGCTGGTGAGATTGA+AGG + chr1.4:24811874-24811893 None:intergenic 50.0%
TTGGACCACCCATAGTAGGT+TGG + chr1.4:24812515-24812534 None:intergenic 50.0%
TTTCTTCTTCGGTCCCACTG+AGG - chr1.4:24812011-24812030 MS.gene59169:intron 50.0%
! CCCCCTAAGTTTTGCAACTG+TGG - chr1.4:24811398-24811417 MS.gene59169:intron 50.0%
! CGCCTAAGGCACTCATGAAT+CGG + chr1.4:24811828-24811847 None:intergenic 50.0%
! GAGCCGACCTCATTTTACTG+TGG - chr1.4:24812257-24812276 MS.gene59169:CDS 50.0%
! GAGTGTTGTTGTTGGTGAGG+TGG + chr1.4:24812060-24812079 None:intergenic 50.0%
! GATGAGTGATGCTGCTGGTT+TGG - chr1.4:24811801-24811820 MS.gene59169:intron 50.0%
!! GCTTCGGTTGGTTTGGGTAT+TGG - chr1.4:24810458-24810477 MS.gene59169:CDS 50.0%
!!! GGTTTTGGACCACCCATAGT+AGG + chr1.4:24812519-24812538 None:intergenic 50.0%
CTGACGTGACACCCTAAGAG+AGG + chr1.4:24811478-24811497 None:intergenic 55.0%
GCGAATTGCAGCTACCTCAG+TGG + chr1.4:24812028-24812047 None:intergenic 55.0%
GGATCTTTCCCTGCCATGGT+TGG + chr1.4:24810152-24810171 None:intergenic 55.0%
GGTAGATTGAGGGAAGGTCC+TGG - chr1.4:24810230-24810249 MS.gene59169:CDS 55.0%
! CCCCCTAAGTTTTGCACGTG+TGG + chr1.4:24811539-24811558 None:intergenic 55.0%
ACTGCAGTTGTAGCCGCGGA+AGG + chr1.4:24812234-24812253 None:intergenic 60.0%
GATTGAGGGAAGGTCCTGGC+AGG - chr1.4:24810234-24810253 MS.gene59169:CDS 60.0%
!! TGGCAGTTTTGGCCCCCCAA+GGG - chr1.4:24811418-24811437 MS.gene59169:intron 60.0%
!! GTGGCAGTTTTGGCCCCCCA+AGG - chr1.4:24811417-24811436 MS.gene59169:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 24809993 24812563 24809993 ID=MS.gene59169
chr1.4 mRNA 24809993 24812563 24809993 ID=MS.gene59169.t1;Parent=MS.gene59169
chr1.4 exon 24812126 24812563 24812126 ID=MS.gene59169.t1.exon1;Parent=MS.gene59169.t1
chr1.4 CDS 24812126 24812563 24812126 ID=cds.MS.gene59169.t1;Parent=MS.gene59169.t1
chr1.4 exon 24809993 24810808 24809993 ID=MS.gene59169.t1.exon2;Parent=MS.gene59169.t1
chr1.4 CDS 24809993 24810808 24809993 ID=cds.MS.gene59169.t1;Parent=MS.gene59169.t1
Gene Sequence

>MS.gene59169

ATGGCCCAATCTCTAGTATTCAAAGTGAAGCGGCGTGTCCTTGAATTTGTAAAACCATCAAAACCAACACCTCATGAAAGAAAATTACTTCCAGACAGGGATGAACAAGATGGATTACGGTATCAAATGCCTGTGATACAATTTTACAACTACGATCCAACCATGGCAGGGAAAGATCCAGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATCATCCATTTGCAGGTAGATTGAGGGAAGGTCCTGGCAGGAAACTTATGGTTGATTGTAATGGTGAAGGTGCTTTGTTCATTGAAGCTGATGCTGATGTTACTCTTATACAATTTGGTGATGCTCTTCATCCTCCATTTCCTTGCTTGGATCAGCTTCTATACGATGTTCCTGGTTCCTCAGATATGCTTAGCACTCCATTGCTGCTTATTCAGGTATAGTTTTTTTAGTCTTCATAATATGCTTCGGTTGGTTTGGGTATTGGGTTTGAAATTTTAAACCAAGATATCTGTGAATCTAATCCGTTGACTTATAGACACATCCGCACATGCGCACAAACACACTATCACACAAATCCCAGGGCGGCCCAATAATTTTGAAAAGAAAATGAGGTCAAAAGTAAGGTTGAATATATGAGGCTTTTTTCCCTTAGAATATGAGGCCTTATTGCTTGTAAAATCTAATGAAAAATATTATTTTCATGCTTTATGCTTTGATGGCCTTAACCTTGAAGCTTTTGCTTTGACGGCCCACCCAATTGATTTCTCGCATGTCCTATGCAGTTCGGATTATATAATTCGAACGACATAATCACCCCATAAAAACCTCCAAAACACGTTAAAATATTTCAGATTTTCGAATCTAAATAGGGTGCACGAGAAATTAATAGGATGAGAAAAATAATGACCTAAAATTAAACCCTGCAATTTAACTGTTTTTTAGAGTTTTCTCTTTGGCATATTTTTAGAACCGTGGTTCAATTCAATTATTTCATGTTGGATAAGTTTGGTTTGATTTTAGTGTTAAATTAAAAGTAGGGTTGGAATTAGTTAATTTAAATTTTTTGTGAAATTCAACCCAACCCAACTTATAAACACTATCTATATACAAGTGACTGAATAATTACCGTTCAACAAAAAAAAATGCTAAATAATGTACACTAAGACAACCTATTTTGTATAAATAAATAAGAGAATTGTACCAAAAAAATAAAAAATAAGAGAAATCAATACCCGCCTCATACATATATTCGAAAAATATGCTAGATCCAAACATAAATATATAACCTAGCTAATGTATTGAACTTGCATTTATTTTAAAAAATAGGCTTAATAGCAGTTTTCGCCCCCTAACTTTCACGAATTTGCGATTTTGGCCCCCAAAGAAAAAACTACAAAACCGCCCCCTAAGTTTTGCAACTGTGGCAGTTTTGGCCCCCCAAGGGCAATTTTAAATAAATAAAAAAAAAATATGTGGCACCTCTCTTAGGGTGTCACGTCAGCGTAGACCAAGTCAAAATTGGTTTTGGGGCCAAAACTGCCACACGTGCAAAACTTAGGGGGCAGTTTTGTAGTTTTTTTTCTTAGGGGGCCAAAATCGCAACTTTGAGAAAGTTTGGGGGATAAAACTGCTATTAAGCCTAAAAAATAATCACATGTGTTTGATTTTGGTCAATTTTTTTTTTTTTTTAAATAGATCAATTATATATGTATTCATATATATTCACATACTTGAAGTGAAATTGTGTGCAGGTAACACGTCTTAAGTGCGGTGGTTTCATTTTTGCTCTTCGTCTAAACCATAGGATGAGTGATGCTGCTGGTTTGGTCCGATTCATGAGTGCCTTAGGCGAAATATCTCATGGGATGAACAAACCTTCAATCTCACCAGCATGGCATAGAGAGCTTCTAAACGCAAGGGATCCTCCAAGAGTAACATGCACTCATCGTGAATATGAACAAGTTCCCGACACCAAAGAAACAATTATCCCTTTACATGCCACAATTCTCTGCTCTTTCTTCTTCGGTCCCACTGAGGTAGCTGCAATTCGCACTCTTCTTCCACCTCACCAACAACAACACTCTAATTTTGAAATCCTCACTGCATTCCTTTGGCGTTGTCGTACAATTGCGTTACAACCAGACATCGATGAAGAAGTTCGCATAGTCAATGCGCATGGCAAGTTTATTAACCCACCCTTACCAAACGGTTACTACGGTAACACTTTTGCATTTCCTTCCGCGGCTACAACTGCAGTAAAGAGCCGACCTCATTTTACTGTGGTGAGAGCATATCTAGTGGTTGATGTTAAACATGCTGGTTTTAGAGAGTTAGATTTCGGTTGGGGAAAACCTTGTTATGGAGGACCAGCTAAAGGAGGAGTTGTTTCTAGTTCGTATATACCTTTTAAAAATGCTAAAGGAGTAGAAGGTTTGGTTATACCGGTTTGTTTGCCAACTAAAGCCATGGAGAGGTTCATTAAAGAGTTGGATAGTGTGCTTAAGAACAACATTAACCAACCTACTATGGGTGGTCCAAAACCTATTTTCATTATTTCTTCCTTGTAG
Protein sequence

>MS.gene59169.t1

MAQSLVFKVKRRVLEFVKPSKPTPHERKLLPDRDEQDGLRYQMPVIQFYNYDPTMAGKDPVDIIRKALAKTLVFYHPFAGRLREGPGRKLMVDCNGEGALFIEADADVTLIQFGDALHPPFPCLDQLLYDVPGSSDMLSTPLLLIQVTRLKCGGFIFALRLNHRMSDAAGLVRFMSALGEISHGMNKPSISPAWHRELLNARDPPRVTCTHREYEQVPDTKETIIPLHATILCSFFFGPTEVAAIRTLLPPHQQQHSNFEILTAFLWRCRTIALQPDIDEEVRIVNAHGKFINPPLPNGYYGNTFAFPSAATTAVKSRPHFTVVRAYLVVDVKHAGFRELDFGWGKPCYGGPAKGGVVSSSYIPFKNAKGVEGLVIPVCLPTKAMERFIKELDSVLKNNINQPTMGGPKPIFIISSL