AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59170.t1 MTR_1g040175 85.995 407 54 1 1 407 55 458 0.0 723
MS.gene59170.t1 MTR_1g040320 85.012 407 25 1 1 407 55 425 0.0 696
MS.gene59170.t1 MTR_1g040215 77.396 407 92 0 1 407 55 461 0.0 669
MS.gene59170.t1 MTR_1g040195 76.042 384 90 2 24 407 55 436 0.0 600
MS.gene59170.t1 MTR_1g040315 64.128 407 102 4 1 407 55 417 0.0 510
MS.gene59170.t1 MTR_0042s0190 74.411 297 76 0 111 407 1 297 3.48e-168 473
MS.gene59170.t1 MTR_7g114560 55.446 404 173 6 1 401 56 455 1.39e-159 457
MS.gene59170.t1 MTR_7g114550 53.827 405 180 6 1 402 56 456 1.20e-158 455
MS.gene59170.t1 MTR_7g114520 55.446 404 161 7 1 394 57 451 1.39e-157 452
MS.gene59170.t1 MTR_5g076700 54.455 404 176 6 1 400 56 455 2.06e-156 449
MS.gene59170.t1 MTR_7g114470 54.187 406 175 7 1 400 57 457 6.99e-152 438
MS.gene59170.t1 MTR_7g114490 55.118 381 165 5 1 378 56 433 1.55e-148 431
MS.gene59170.t1 MTR_1g081660 55.928 388 166 5 1 386 64 448 1.94e-146 424
MS.gene59170.t1 MTR_0042s0190 75.431 232 57 0 176 407 1 232 1.35e-127 367
MS.gene59170.t1 MTR_7g114510 51.768 311 132 6 93 394 8 309 9.45e-106 315
MS.gene59170.t1 MTR_0502s0010 58.436 243 93 4 46 284 1 239 1.67e-96 288
MS.gene59170.t1 MTR_3g035000 63.063 222 55 3 145 365 43 238 1.49e-88 271
MS.gene59170.t1 MTR_3g098980 34.646 381 235 8 2 378 58 428 4.77e-78 248
MS.gene59170.t1 MTR_7g114420 44.027 293 131 5 111 400 1 263 3.72e-77 240
MS.gene59170.t1 MTR_8g096530 33.773 379 236 8 3 376 52 420 2.84e-74 238
MS.gene59170.t1 MTR_7g027175 34.121 381 234 9 2 376 60 429 5.04e-73 235
MS.gene59170.t1 MTR_8g075610 31.053 380 218 9 9 366 46 403 2.73e-52 180
MS.gene59170.t1 MTR_4g007540 30.997 371 234 9 9 366 55 416 4.18e-52 180
MS.gene59170.t1 MTR_0042s0230 87.097 93 12 0 1 93 55 147 2.85e-51 169
MS.gene59170.t1 MTR_2g105330 30.479 397 230 12 9 382 55 428 2.27e-50 176
MS.gene59170.t1 MTR_2g064495 30.570 386 244 10 9 382 55 428 1.41e-49 173
MS.gene59170.t1 MTR_3g112180 33.043 345 209 11 7 337 58 394 1.10e-48 172
MS.gene59170.t1 MTR_1g053315 30.179 391 258 9 8 386 76 463 1.31e-48 172
MS.gene59170.t1 MTR_4g055170 29.008 393 264 8 4 383 63 453 2.99e-47 168
MS.gene59170.t1 MTR_8g039950 91.892 74 6 0 1 74 55 128 5.32e-41 142
MS.gene59170.t1 MTR_7g086000 30.084 359 209 13 7 331 57 407 3.74e-40 149
MS.gene59170.t1 MTR_3g465990 30.221 407 238 15 9 385 67 457 1.07e-39 147
MS.gene59170.t1 MTR_7g114500 62.887 97 36 0 1 97 56 152 4.21e-39 137
MS.gene59170.t1 MTR_7g056087 29.199 387 241 12 13 380 61 433 4.55e-39 145
MS.gene59170.t1 MTR_6g053810 26.290 407 270 9 2 385 63 462 2.66e-38 144
MS.gene59170.t1 MTR_7g110610 30.220 364 190 12 3 357 48 356 3.47e-38 142
MS.gene59170.t1 MTR_2g015430 30.149 335 225 7 5 333 53 384 5.21e-38 142
MS.gene59170.t1 MTR_3g071320 30.347 346 213 12 10 337 60 395 6.31e-38 142
MS.gene59170.t1 MTR_7g084940 29.167 336 223 8 10 337 69 397 8.36e-38 142
MS.gene59170.t1 MTR_3g051790 64.583 96 34 0 1 96 59 154 1.51e-36 132
MS.gene59170.t1 MTR_3g465980 29.529 403 246 14 9 385 67 457 2.00e-36 139
MS.gene59170.t1 MTR_4g127650 28.652 356 220 10 10 338 66 414 3.67e-36 138
MS.gene59170.t1 MTR_3g465930 28.986 414 245 14 5 385 64 461 8.00e-36 137
MS.gene59170.t1 MTR_7g085990 27.536 414 246 16 7 380 52 451 9.80e-36 137
MS.gene59170.t1 MTR_3g465910 31.959 291 178 7 5 283 63 345 1.42e-35 137
MS.gene59170.t1 MTR_7g084910 28.739 341 204 10 10 337 70 384 5.88e-35 134
MS.gene59170.t1 MTR_7g085930 27.200 375 219 13 7 337 56 420 2.52e-34 133
MS.gene59170.t1 MTR_3g026950 26.648 364 230 9 7 338 51 409 4.63e-33 129
MS.gene59170.t1 MTR_4g097090 27.013 385 242 11 10 366 65 438 1.21e-32 128
MS.gene59170.t1 MTR_3g465950 30.847 295 185 9 2 283 60 348 9.78e-32 125
MS.gene59170.t1 MTR_3g465880 28.467 411 261 14 2 385 60 464 3.87e-30 121
MS.gene59170.t1 MTR_5g090830 26.485 404 229 14 7 373 63 435 4.56e-29 118
MS.gene59170.t1 MTR_5g090810 25.815 399 238 13 7 373 63 435 4.89e-28 115
MS.gene59170.t1 MTR_3g049150 25.210 357 235 9 7 336 52 403 1.15e-26 111
MS.gene59170.t1 MTR_5g090880 25.250 400 240 14 7 373 64 437 3.05e-25 107
MS.gene59170.t1 MTR_4g013050 23.704 405 214 15 10 376 53 400 1.99e-24 104
MS.gene59170.t1 MTR_3g465870 28.854 253 160 8 2 241 62 307 9.92e-24 102
MS.gene59170.t1 MTR_3g450710 25.576 434 242 17 10 400 65 460 4.16e-23 101
MS.gene59170.t1 MTR_3g450690 25.116 430 246 16 10 400 72 464 4.56e-22 98.2
MS.gene59170.t1 MTR_1g094790 24.745 392 228 15 10 365 60 420 4.27e-20 92.0
MS.gene59170.t1 MTR_4g094842 26.027 365 227 14 10 345 62 412 9.48e-20 91.3
MS.gene59170.t1 MTR_4g094828 23.929 397 268 13 5 378 54 439 1.82e-19 90.5
MS.gene59170.t1 MTR_3g101930 27.717 368 212 17 10 343 70 417 1.32e-18 87.8
MS.gene59170.t1 MTR_8g079710 24.929 353 229 14 5 333 54 394 4.24e-18 86.3
MS.gene59170.t1 MTR_8g041770 26.099 364 217 15 5 341 59 397 7.14e-18 85.5
MS.gene59170.t1 MTR_5g084900 25.000 336 197 15 10 334 9 300 1.41e-17 84.0
MS.gene59170.t1 MTR_0190s0020 24.297 391 252 17 10 378 63 431 1.73e-17 84.3
MS.gene59170.t1 MTR_3g101900 24.878 410 259 16 7 391 63 448 3.99e-17 83.2
MS.gene59170.t1 MTR_3g088420 24.691 405 252 18 5 383 50 427 4.16e-17 83.2
MS.gene59170.t1 MTR_7g026860 24.495 396 259 16 5 378 50 427 8.69e-16 79.3
MS.gene59170.t1 MTR_4g094838 22.071 367 237 12 2 345 56 396 1.20e-15 78.6
MS.gene59170.t1 MTR_5g084870 21.910 356 210 13 10 334 59 377 1.20e-15 78.6
MS.gene59170.t1 MTR_4g022800 24.862 362 240 14 5 341 50 404 1.32e-15 78.6
MS.gene59170.t1 MTR_7g114417 65.385 52 17 1 35 85 29 80 1.33e-15 72.0
MS.gene59170.t1 MTR_3g102010 26.385 379 222 16 7 348 62 420 2.66e-15 77.8
MS.gene59170.t1 MTR_3g102020 25.729 377 223 15 7 348 66 420 2.88e-15 77.8
MS.gene59170.t1 MTR_2g036780 24.740 384 243 12 18 383 74 429 4.39e-15 77.0
MS.gene59170.t1 MTR_8g079970 21.591 352 222 9 10 334 59 383 2.94e-14 74.3
MS.gene59170.t1 MTR_4g022825 24.570 407 251 17 3 378 48 429 3.69e-14 74.3
MS.gene59170.t1 MTR_3g051820 47.917 96 44 4 313 407 1 91 5.78e-14 67.8
MS.gene59170.t1 MTR_5g084850 20.294 340 234 10 10 334 59 376 1.56e-13 72.4
MS.gene59170.t1 MTR_1g035890 25.065 383 225 15 25 382 82 427 2.23e-13 71.6
MS.gene59170.t1 MTR_8g079980 25.764 229 137 9 10 224 59 268 6.87e-13 70.1
MS.gene59170.t1 MTR_4g022760 27.007 274 182 9 10 269 55 324 1.40e-12 69.3
MS.gene59170.t1 MTR_3g049140 31.333 150 84 5 203 336 54 200 7.44e-12 65.5
MS.gene59170.t1 MTR_3g100150 24.101 278 183 10 21 287 76 336 1.74e-11 66.2
MS.gene59170.t1 MTR_4g007460 30.496 141 88 4 24 164 1 131 7.00e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59170.t1 AT5G17540 54.146 410 175 8 5 407 58 461 2.74e-148 429
MS.gene59170.t1 AT3G03480 51.804 388 175 7 5 386 68 449 5.14e-133 389
MS.gene59170.t1 AT5G41040 37.360 356 203 9 4 350 63 407 2.06e-79 252
MS.gene59170.t1 AT5G41040 37.151 358 205 9 2 350 77 423 2.59e-79 252
MS.gene59170.t1 AT5G63560 34.817 382 226 10 2 376 53 418 3.19e-75 240
MS.gene59170.t1 AT3G48720 31.053 380 247 9 2 376 53 422 2.32e-69 225
MS.gene59170.t1 AT5G48930 30.645 372 234 9 9 366 55 416 8.72e-51 177
MS.gene59170.t1 AT3G62160 33.239 352 202 12 3 340 48 380 2.70e-47 167
MS.gene59170.t1 AT1G28680 28.928 401 263 10 1 387 53 445 2.20e-46 165
MS.gene59170.t1 AT1G03390 32.849 344 207 11 10 337 76 411 5.42e-46 164
MS.gene59170.t1 AT1G27620 29.219 397 258 10 10 396 58 441 1.62e-44 160
MS.gene59170.t1 AT5G57840 29.859 355 216 9 12 343 58 402 4.37e-44 159
MS.gene59170.t1 AT1G65450 31.078 399 232 13 10 386 72 449 2.42e-42 154
MS.gene59170.t1 AT2G19070 30.748 361 215 12 7 338 55 409 5.83e-42 154
MS.gene59170.t1 AT1G28680 30.124 322 212 7 1 314 53 369 7.12e-42 152
MS.gene59170.t1 AT2G40230 30.851 376 225 14 10 367 59 417 9.46e-41 150
MS.gene59170.t1 AT2G23510 29.803 406 239 14 6 385 65 450 7.18e-38 142
MS.gene59170.t1 AT5G07080 28.500 400 231 15 5 375 65 438 2.78e-35 135
MS.gene59170.t1 AT3G47170 27.536 414 254 14 6 386 66 466 4.79e-35 135
MS.gene59170.t1 AT2G25150 27.387 398 269 10 5 387 62 454 6.12e-35 134
MS.gene59170.t1 AT1G78990 27.551 392 227 13 8 377 65 421 4.06e-33 129
MS.gene59170.t1 AT1G32910 28.148 405 250 14 8 387 63 451 5.24e-33 129
MS.gene59170.t1 AT1G31490 27.635 351 209 15 7 335 59 386 6.33e-26 109
MS.gene59170.t1 AT4G31910 29.261 352 210 13 10 330 64 407 1.33e-25 108
MS.gene59170.t1 AT1G24420 26.126 333 201 12 20 334 75 380 7.56e-22 97.4
MS.gene59170.t1 AT1G24420 26.126 333 201 12 20 334 75 380 7.56e-22 97.4
MS.gene59170.t1 AT5G16410 25.212 353 221 13 8 336 89 422 1.03e-21 97.4
MS.gene59170.t1 AT5G38130 27.606 355 224 15 13 345 82 425 1.30e-21 97.1
MS.gene59170.t1 AT5G07860 25.510 392 252 12 10 383 72 441 1.46e-20 93.6
MS.gene59170.t1 AT5G07850 26.316 361 236 13 10 348 70 422 2.33e-20 93.2
MS.gene59170.t1 AT3G50270 25.140 358 239 12 10 345 62 412 2.24e-19 90.1
MS.gene59170.t1 AT1G65450 27.063 303 180 11 105 386 3 285 4.33e-19 87.4
MS.gene59170.t1 AT3G50280 25.141 354 242 11 10 345 59 407 4.79e-19 89.0
MS.gene59170.t1 AT5G42830 27.586 377 219 14 7 348 61 418 1.17e-18 87.8
MS.gene59170.t1 AT5G23940 24.631 406 262 13 7 386 62 449 2.67e-18 87.0
MS.gene59170.t1 AT1G24430 23.352 364 220 13 1 339 53 382 3.65e-18 86.3
MS.gene59170.t1 AT5G67160 25.553 407 246 14 4 383 50 426 1.53e-17 84.3
MS.gene59170.t1 AT4G15390 23.714 350 219 14 10 334 64 390 5.67e-16 79.7
MS.gene59170.t1 AT4G15390 23.714 350 219 14 10 334 64 390 5.67e-16 79.7
MS.gene59170.t1 AT3G23840 25.430 291 169 9 26 299 81 340 1.25e-14 75.5
MS.gene59170.t1 AT3G30280 22.286 350 223 13 10 334 62 387 2.95e-14 74.7
MS.gene59170.t1 AT3G29670 24.207 347 230 15 10 333 66 402 1.09e-12 69.7
MS.gene59170.t1 AT2G39980 28.889 135 94 2 10 143 62 195 9.83e-11 63.9

Find 93 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCCATTTCCTTTCT+TGG 0.075005 1.4:-24820357 MS.gene59170:CDS
TGCAGCAATGATTTCAAAAT+TGG 0.167630 1.4:+24818255 None:intergenic
TCTCCTTTATCATTTGTAAA+AGG 0.217002 1.4:+24817806 None:intergenic
ACTAAGGGTGATCACAAATT+TGG 0.227915 1.4:-24817686 MS.gene59170:CDS
GATGTTACTCTTAAAGAATT+TGG 0.276060 1.4:-24820389 MS.gene59170:CDS
GGATTTAGAGATGTTGATTT+TGG 0.298021 1.4:-24817905 MS.gene59170:CDS
GTTCCTTTGTTGTCGGGTTC+AGG 0.305288 1.4:+24818382 None:intergenic
GAGCTTCTTTATGATGTTCC+TGG 0.318598 1.4:-24820332 MS.gene59170:CDS
TATAATCCGGCAGTAGTTAA+AGG 0.320164 1.4:+24817953 None:intergenic
AAATCATTGCTGCATACTTT+TGG 0.324455 1.4:-24818246 MS.gene59170:CDS
CAACTTCCAAATGGTTACTA+TGG 0.337065 1.4:-24818133 MS.gene59170:CDS
GGAGGGGATGGTGCTCTTCC+TGG 0.355512 1.4:-24817848 MS.gene59170:CDS
GTTCACCACACTTTCTTCAT+TGG 0.359940 1.4:-24818337 MS.gene59170:CDS
CCTGCAGTTGTAACCGCAAC+GGG 0.366367 1.4:+24818094 None:intergenic
CCCGTTGCGGTTACAACTGC+AGG 0.385362 1.4:-24818094 MS.gene59170:CDS
TGATGAGTACATGTCACTTT+TGG 0.395312 1.4:+24818415 None:intergenic
ACAAATGATAAAGGAGAGAA+AGG 0.408216 1.4:-24817800 MS.gene59170:CDS
AGCTCATCCAAGAAAGGAAA+TGG 0.411901 1.4:+24820350 None:intergenic
AACATGGTTCCTTTGTTGTC+GGG 0.413970 1.4:+24818376 None:intergenic
CTCCACACCGGTGTGATTGA+AGG 0.417124 1.4:+24818466 None:intergenic
AATTTGTGATCACCCTTAGT+AGG 0.417980 1.4:+24817689 None:intergenic
GTGGTGAACCATGTCGTCTA+AGG 0.420378 1.4:+24818351 None:intergenic
AGGTCCTAGTAGGAAACTTA+TGG 0.435900 1.4:-24820456 MS.gene59170:CDS
AGTAACCATTTGGAAGTTGT+TGG 0.444242 1.4:+24818137 None:intergenic
CAACATTAACCAGCCTACTA+AGG 0.446515 1.4:-24817702 MS.gene59170:CDS
GTGTCTGATGTTACGCGTGC+TGG 0.446593 1.4:-24817926 MS.gene59170:CDS
AATTGGAGTATTGTTGCAAT+TGG 0.448429 1.4:+24818272 None:intergenic
GCGTTACCATAGTAACCATT+TGG 0.448893 1.4:+24818127 None:intergenic
CACTCCATTGCTACTTATCC+AGG 0.458160 1.4:-24820291 MS.gene59170:intron
CGTCCTCCTTTAACTACTGC+CGG 0.461233 1.4:-24817959 MS.gene59170:CDS
ATCCATTTGCAGGTAGATTG+AGG 0.464119 1.4:-24820481 MS.gene59170:CDS
GGAGAGGTTGGTTAAAGAGT+TGG 0.469038 1.4:-24817741 MS.gene59170:CDS
TGTGATCACCCTTAGTAGGC+TGG 0.470105 1.4:+24817693 None:intergenic
TCCCTCAATCTACCTGCAAA+TGG 0.471845 1.4:+24820479 None:intergenic
AACCATGATCCAAATATGGC+AGG 0.473833 1.4:-24820560 None:intergenic
CTGATGCATCACTCATTGTA+TGG 0.478329 1.4:+24818542 None:intergenic
AAACATGGTTCCTTTGTTGT+CGG 0.483476 1.4:+24818375 None:intergenic
AAAACTTATTGAAAATCCAT+TGG 0.488114 1.4:-24818071 MS.gene59170:CDS
TCCATTTGCAGGTAGATTGA+GGG 0.488896 1.4:-24820480 MS.gene59170:CDS
GCACCATCCCCTCCTTTAGC+TGG 0.492161 1.4:+24817857 None:intergenic
CGCTTTAGCATTTCCCGTTG+CGG 0.494082 1.4:-24818107 MS.gene59170:CDS
GATTGAGGGAAGGTCCTAGT+AGG 0.496365 1.4:-24820466 MS.gene59170:CDS
ATCAGACACTAAATATAATC+CGG 0.507875 1.4:+24817940 None:intergenic
AACATATTTGCTACGTGCAT+CGG 0.510191 1.4:+24818162 None:intergenic
GTTGACCAACAACTTCCAAA+TGG 0.511375 1.4:-24818142 MS.gene59170:CDS
TATTGTTGCAATTGGTGAGT+TGG 0.518445 1.4:+24818280 None:intergenic
TGATAAAGGAGAGAAAGGTT+TGG 0.523809 1.4:-24817795 MS.gene59170:CDS
AACTCAAGTCATGGAGAGGT+TGG 0.531928 1.4:-24817753 MS.gene59170:CDS
TAAAGAAGCTCATCCAAGAA+AGG 0.532338 1.4:+24820344 None:intergenic
AACCTCTCCATGACTTGAGT+TGG 0.536373 1.4:+24817755 None:intergenic
CATGTTTCCCTTAGACGACA+TGG 0.537703 1.4:-24818359 MS.gene59170:CDS
CCCGCGAGATATCTCTCCTA+AGG 0.540283 1.4:+24818498 None:intergenic
TCCTGCAGTTGTAACCGCAA+CGG 0.546523 1.4:+24818093 None:intergenic
ACCATGATCCAAATATGGCA+GGG 0.552731 1.4:-24820559 None:intergenic
GCAGAGAGCTTCTTAACGCA+AGG 0.556473 1.4:-24818444 MS.gene59170:CDS
GTTTATGGAGGACCAGCTAA+AGG 0.560640 1.4:-24817869 MS.gene59170:CDS
CATACAATGAGTGATGCATC+AGG 0.566369 1.4:-24818541 MS.gene59170:CDS
AAATTCAATGCATACTCCAA+TGG 0.567686 1.4:+24818055 None:intergenic
TCCACCATAAGTTTCCTACT+AGG 0.568105 1.4:+24820452 None:intergenic
AAACTTATGGTGGATTGTAA+TGG 0.572360 1.4:-24820443 MS.gene59170:CDS
CGAATTGCAGCAACCTCGGT+GGG 0.573531 1.4:+24818313 None:intergenic
TGCCAACTCAAGTCATGGAG+AGG 0.581695 1.4:-24817757 MS.gene59170:CDS
ACAACTAGATTCTAACGAAG+AGG 0.582842 1.4:-24818203 MS.gene59170:CDS
TGGTGAACCATGTCGTCTAA+GGG 0.584264 1.4:+24818352 None:intergenic
AACATTAACCAGCCTACTAA+GGG 0.592477 1.4:-24817701 MS.gene59170:CDS
GGACCAGCTAAAGGAGGGGA+TGG 0.594000 1.4:-24817860 MS.gene59170:CDS
AGAGCGAATTGCAGCAACCT+CGG 0.595184 1.4:+24818309 None:intergenic
CATGTCGTCTAAGGGAAACA+TGG 0.598974 1.4:+24818360 None:intergenic
ATGGAGGACCAGCTAAAGGA+GGG 0.603039 1.4:-24817865 MS.gene59170:CDS
ATATGAAAGACAGCTTGACC+AGG 0.606019 1.4:+24817830 None:intergenic
TATACCTGGATAAGTAGCAA+TGG 0.608440 1.4:+24820287 None:intergenic
GTTGCAATTGGTGAGTTGGA+AGG 0.610618 1.4:+24818284 None:intergenic
GCCTTAGGAGAGATATCTCG+CGG 0.618905 1.4:-24818499 MS.gene59170:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.622508 1.4:-24817866 MS.gene59170:CDS
CAGAGAGCTTCTTAACGCAA+GGG 0.631889 1.4:-24818443 MS.gene59170:CDS
GAACCTGAACCCGACAACAA+AGG 0.632322 1.4:-24818385 MS.gene59170:CDS
CAAAGAGTATATGCATTCAG+TGG 0.634352 1.4:-24818005 MS.gene59170:CDS
TCCTAGTAGGAAACTTATGG+TGG 0.635661 1.4:-24820453 MS.gene59170:CDS
CAGGTAACACGCTTCAAGTG+TGG 0.636908 1.4:-24818592 MS.gene59170:CDS
GTAACACGCTTCAAGTGTGG+TGG 0.643774 1.4:-24818589 MS.gene59170:CDS
AACCTTCAATCACACCGGTG+TGG 0.648198 1.4:-24818468 MS.gene59170:CDS
TCATCCAAGAAAGGAAATGG+AGG 0.650133 1.4:+24820353 None:intergenic
AGAAGCTCTCTGCTCCACAC+CGG 0.657482 1.4:+24818454 None:intergenic
ATGGTGGATTGTAATGGTGA+AGG 0.660354 1.4:-24820437 MS.gene59170:CDS
TTAATCACATCTGAAGAACC+AGG 0.665982 1.4:+24820314 None:intergenic
TTTGCAGGTAGATTGAGGGA+AGG 0.669320 1.4:-24820476 MS.gene59170:CDS
GCGAATTGCAGCAACCTCGG+TGG 0.672845 1.4:+24818312 None:intergenic
AATCCGGCAGTAGTTAAAGG+AGG 0.679429 1.4:+24817956 None:intergenic
GAGTAAACCTTCAATCACAC+CGG 0.681995 1.4:-24818473 MS.gene59170:CDS
CCTTAGGAGAGATATCTCGC+GGG 0.690826 1.4:-24818498 MS.gene59170:CDS
TGGAGGACCAGCTAAAGGAG+GGG 0.700713 1.4:-24817864 MS.gene59170:CDS
TTTCTTCATTGGTCCCACCG+AGG 0.710851 1.4:-24818326 MS.gene59170:CDS
TGGGACCAATGAAGAAAGTG+TGG 0.712278 1.4:+24818332 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATAATAAAATATTTTATGT+TGG + chr1.4:24819062-24819081 None:intergenic 10.0%
!!! TAAATTATTTTAATAAGTGA+AGG + chr1.4:24819365-24819384 None:intergenic 10.0%
!!! TATTTGAATCATTTTTTTTA+AGG + chr1.4:24819427-24819446 None:intergenic 10.0%
!! AAAAATGATTCAAATATATG+TGG - chr1.4:24819431-24819450 MS.gene59170:intron 15.0%
!! TATTAAAATCAATTGATATG+CGG + chr1.4:24819560-24819579 None:intergenic 15.0%
!! TCCTCAAAAAAAAAAAAAAA+AGG - chr1.4:24818861-24818880 MS.gene59170:intron 15.0%
!!! AAAAAACCTTATTTTCTATA+TGG + chr1.4:24818842-24818861 None:intergenic 15.0%
!!! AATAATTTAAACTTTTTGTG+AGG - chr1.4:24819376-24819395 MS.gene59170:intron 15.0%
!!! ACCTTTTTTTTTTTTTTTTG+AGG + chr1.4:24818865-24818884 None:intergenic 15.0%
!!! CAATTTATTTTATCTTTTCA+AGG - chr1.4:24819101-24819120 MS.gene59170:intron 15.0%
!! AAAACTTATTGAAAATCCAT+TGG - chr1.4:24820138-24820157 MS.gene59170:intron 20.0%
!! ATACAAAATTATCCTCATTT+AGG + chr1.4:24818445-24818464 None:intergenic 20.0%
!! GATTTATGTTCCATTATTAT+AGG + chr1.4:24818267-24818286 None:intergenic 20.0%
!! TACAAAATTATCCTCATTTA+GGG + chr1.4:24818444-24818463 None:intergenic 20.0%
!! TGTAAAAAATGGTCATTAAT+TGG + chr1.4:24819459-24819478 None:intergenic 20.0%
!!! ATCAAATTTTGAGATCATTT+TGG + chr1.4:24819284-24819303 None:intergenic 20.0%
!!! ATCAAGAGTATTTAAAAGTT+TGG + chr1.4:24819240-24819259 None:intergenic 20.0%
!!! ATGAAAATTACACATTTTAC+AGG + chr1.4:24818974-24818993 None:intergenic 20.0%
!!! ATTCCTTTTACAAATGATAA+AGG - chr1.4:24820400-24820419 MS.gene59170:CDS 20.0%
!!! ATTTTAGTTTCTTGAGTTTT+CGG - chr1.4:24819316-24819335 MS.gene59170:intron 20.0%
!!! GATCTCAAAATTTGATTTTT+AGG - chr1.4:24819288-24819307 MS.gene59170:intron 20.0%
!!! TATTGAAGAAAAGTTTTGAA+GGG + chr1.4:24818408-24818427 None:intergenic 20.0%
!!! TCTAGGTTTTAATATCTTTT+TGG + chr1.4:24819183-24819202 None:intergenic 20.0%
!!! TGAAAATTACACATTTTACA+GGG + chr1.4:24818973-24818992 None:intergenic 20.0%
!!! TTCAATTCATTTTAACTTCT+AGG + chr1.4:24819200-24819219 None:intergenic 20.0%
! AAGAAATAGATAACATTAGG+AGG + chr1.4:24818500-24818519 None:intergenic 25.0%
! AATCTATGTGGTGTAAAAAA+TGG + chr1.4:24819470-24819489 None:intergenic 25.0%
! AGAAATAGATAACATTAGGA+GGG + chr1.4:24818499-24818518 None:intergenic 25.0%
! ATCAGACACTAAATATAATC+CGG + chr1.4:24820272-24820291 None:intergenic 25.0%
! CATTATTATAGGAAAACATG+GGG + chr1.4:24818256-24818275 None:intergenic 25.0%
! CCATTATTATAGGAAAACAT+GGG + chr1.4:24818257-24818276 None:intergenic 25.0%
! GATGTTACTCTTAAAGAATT+TGG - chr1.4:24817820-24817839 MS.gene59170:CDS 25.0%
! GCTTTGTGGAAAAATATAAA+GGG - chr1.4:24818067-24818086 MS.gene59170:CDS 25.0%
! TACATATTATCGGAGAAATT+TGG - chr1.4:24817963-24817982 MS.gene59170:CDS 25.0%
! TCCATTATTATAGGAAAACA+TGG + chr1.4:24818258-24818277 None:intergenic 25.0%
! TCTCCTTTATCATTTGTAAA+AGG + chr1.4:24820406-24820425 None:intergenic 25.0%
! TGGAAGAAATAGATAACATT+AGG + chr1.4:24818503-24818522 None:intergenic 25.0%
! TTTAGGCCATATAGAAAATA+AGG - chr1.4:24818833-24818852 MS.gene59170:intron 25.0%
!! AAAATGAATTGAAGCCTTAT+CGG - chr1.4:24819207-24819226 MS.gene59170:intron 25.0%
!! AGCTTTGTGGAAAAATATAA+AGG - chr1.4:24818066-24818085 MS.gene59170:CDS 25.0%
!! CTTTTCACACTACATATTAT+CGG - chr1.4:24817953-24817972 MS.gene59170:CDS 25.0%
!! GCTTTTCTAATAATATCAAC+AGG + chr1.4:24817682-24817701 None:intergenic 25.0%
!!! ATAGTTTTGGAAAAAAGGAA+AGG + chr1.4:24818532-24818551 None:intergenic 25.0%
!!! GTATTGAAGAAAAGTTTTGA+AGG + chr1.4:24818409-24818428 None:intergenic 25.0%
!!! GTCACATGTTCAATTTTTTA+AGG + chr1.4:24818314-24818333 None:intergenic 25.0%
AAATTCAATGCATACTCCAA+TGG + chr1.4:24820157-24820176 None:intergenic 30.0%
AACATAGCAATGTAACATCA+AGG + chr1.4:24819504-24819523 None:intergenic 30.0%
ACAAATGATAAAGGAGAGAA+AGG - chr1.4:24820409-24820428 MS.gene59170:CDS 30.0%
GAAAAGATCAATACTATACC+TGG + chr1.4:24817939-24817958 None:intergenic 30.0%
GAAATAGATAACATTAGGAG+GGG + chr1.4:24818498-24818517 None:intergenic 30.0%
GAGAAACAATTCGCTATTAT+AGG + chr1.4:24818289-24818308 None:intergenic 30.0%
GTAGAAAGTTCAAATCTATG+TGG + chr1.4:24819482-24819501 None:intergenic 30.0%
GTCAAAATTCAATGCAGATT+TGG + chr1.4:24818685-24818704 None:intergenic 30.0%
GTCCTTTCAATACTTATGTA+AGG + chr1.4:24819003-24819022 None:intergenic 30.0%
TGCAGCAATGATTTCAAAAT+TGG + chr1.4:24819957-24819976 None:intergenic 30.0%
! AAATCATTGCTGCATACTTT+TGG - chr1.4:24819963-24819982 MS.gene59170:intron 30.0%
! CCCATGTTTTCCTATAATAA+TGG - chr1.4:24818254-24818273 MS.gene59170:CDS 30.0%
! GCAGATTTTATGGTTATCAA+GGG - chr1.4:24820226-24820245 MS.gene59170:intron 30.0%
!! AAACTTATGGTGGATTGTAA+TGG - chr1.4:24817766-24817785 MS.gene59170:CDS 30.0%
!! AATTGGAGTATTGTTGCAAT+TGG + chr1.4:24819940-24819959 None:intergenic 30.0%
!! GTCCTTACATAAGTATTGAA+AGG - chr1.4:24818998-24819017 MS.gene59170:intron 30.0%
!! GTGTTTTATTATCCATTTGC+AGG - chr1.4:24817718-24817737 MS.gene59170:CDS 30.0%
!!! GGATTTAGAGATGTTGATTT+TGG - chr1.4:24820304-24820323 MS.gene59170:CDS 30.0%
!!! TAGAGATGTTGATTTTGGTT+GGG - chr1.4:24820309-24820328 MS.gene59170:CDS 30.0%
!!! TTAGAGATGTTGATTTTGGT+TGG - chr1.4:24820308-24820327 MS.gene59170:CDS 30.0%
AACATTAACCAGCCTACTAA+GGG - chr1.4:24820508-24820527 MS.gene59170:CDS 35.0%
AATTTGTGATCACCCTTAGT+AGG + chr1.4:24820523-24820542 None:intergenic 35.0%
ACAAATGTTCACCTAAGCTT+TGG + chr1.4:24818583-24818602 None:intergenic 35.0%
ACAACTAGATTCTAACGAAG+AGG - chr1.4:24820006-24820025 MS.gene59170:intron 35.0%
ACATTTGTATCGAGATCACA+CGG - chr1.4:24818595-24818614 MS.gene59170:intron 35.0%
ACCAATAGAAACAATTCGCT+AGG + chr1.4:24818215-24818234 None:intergenic 35.0%
ACTAAGGGTGATCACAAATT+TGG - chr1.4:24820523-24820542 MS.gene59170:CDS 35.0%
ACTTCTTAGTTCCCTAAATG+AGG - chr1.4:24818430-24818449 MS.gene59170:CDS 35.0%
AGTAACCATTTGGAAGTTGT+TGG + chr1.4:24820075-24820094 None:intergenic 35.0%
ATATGCGGTGATTAAACTTG+AGG + chr1.4:24819545-24819564 None:intergenic 35.0%
CAACTTCCAAATGGTTACTA+TGG - chr1.4:24820076-24820095 MS.gene59170:intron 35.0%
TAAAGAAGCTCATCCAAGAA+AGG + chr1.4:24817868-24817887 None:intergenic 35.0%
TATAATCCGGCAGTAGTTAA+AGG + chr1.4:24820259-24820278 None:intergenic 35.0%
TATACCTGGATAAGTAGCAA+TGG + chr1.4:24817925-24817944 None:intergenic 35.0%
TATTGTTGCAATTGGTGAGT+TGG + chr1.4:24819932-24819951 None:intergenic 35.0%
TGATAAAGGAGAGAAAGGTT+TGG - chr1.4:24820414-24820433 MS.gene59170:CDS 35.0%
TTAATCACATCTGAAGAACC+AGG + chr1.4:24817898-24817917 None:intergenic 35.0%
TTTCGTTGGATCGAAATCAA+AGG + chr1.4:24818709-24818728 None:intergenic 35.0%
! AAACATGGTTCCTTTGTTGT+CGG + chr1.4:24819837-24819856 None:intergenic 35.0%
! AACATATTTGCTACGTGCAT+CGG + chr1.4:24820050-24820069 None:intergenic 35.0%
! AGTTTGGTAGTATTCCGATA+AGG + chr1.4:24819224-24819243 None:intergenic 35.0%
! CAAAGAGTATATGCATTCAG+TGG - chr1.4:24820204-24820223 MS.gene59170:intron 35.0%
! GGCAGATTTTATGGTTATCA+AGG - chr1.4:24820225-24820244 MS.gene59170:intron 35.0%
!! ACTTGAGTTGGCAAAAAAAC+TGG + chr1.4:24820445-24820464 None:intergenic 35.0%
!! AGCGAATTGTTTCTATTGGT+TGG - chr1.4:24818215-24818234 MS.gene59170:CDS 35.0%
!!! AGAGATGTTGATTTTGGTTG+GGG - chr1.4:24820310-24820329 MS.gene59170:CDS 35.0%
!!! AGGGGATAGTTTTGGAAAAA+AGG + chr1.4:24818537-24818556 None:intergenic 35.0%
!!! CTTCAATTTTTGAGTGCCTT+AGG - chr1.4:24819695-24819714 MS.gene59170:intron 35.0%
!!! TGATGAGTACATGTCACTTT+TGG + chr1.4:24819797-24819816 None:intergenic 35.0%
!!! TTTTTTGCCAACTCAAGTCA+TGG - chr1.4:24820447-24820466 MS.gene59170:CDS 35.0%
AATTCATTTCTAACCCGTGC+AGG - chr1.4:24819598-24819617 MS.gene59170:intron 40.0%
AGCTCATCCAAGAAAGGAAA+TGG + chr1.4:24817862-24817881 None:intergenic 40.0%
AGGTCCTAGTAGGAAACTTA+TGG - chr1.4:24817753-24817772 MS.gene59170:CDS 40.0%
ATAGATAACATTAGGAGGGG+AGG + chr1.4:24818495-24818514 None:intergenic 40.0%
ATATGAAAGACAGCTTGACC+AGG + chr1.4:24820382-24820401 None:intergenic 40.0%
ATCCATTTGCAGGTAGATTG+AGG - chr1.4:24817728-24817747 MS.gene59170:CDS 40.0%
ATTTCGATCCAACGAAACGA+TGG - chr1.4:24818712-24818731 MS.gene59170:intron 40.0%
CAAACTTGCAACCAAAGCTT+AGG - chr1.4:24818569-24818588 MS.gene59170:CDS 40.0%
CAACATTAACCAGCCTACTA+AGG - chr1.4:24820507-24820526 MS.gene59170:CDS 40.0%
CATACAATGAGTGATGCATC+AGG - chr1.4:24819668-24819687 MS.gene59170:intron 40.0%
CTGATGCATCACTCATTGTA+TGG + chr1.4:24819670-24819689 None:intergenic 40.0%
GAAAAAAGGAAAGGAGCAAG+TGG + chr1.4:24818523-24818542 None:intergenic 40.0%
GAGCTTCTTTATGATGTTCC+TGG - chr1.4:24817877-24817896 MS.gene59170:CDS 40.0%
GAGTAAACCTTCAATCACAC+CGG - chr1.4:24819736-24819755 MS.gene59170:intron 40.0%
GCGTTACCATAGTAACCATT+TGG + chr1.4:24820085-24820104 None:intergenic 40.0%
GTTCACCACACTTTCTTCAT+TGG - chr1.4:24819872-24819891 MS.gene59170:intron 40.0%
GTTGACCAACAACTTCCAAA+TGG - chr1.4:24820067-24820086 MS.gene59170:intron 40.0%
TAGATAACATTAGGAGGGGA+GGG + chr1.4:24818494-24818513 None:intergenic 40.0%
TCATCCAAGAAAGGAAATGG+AGG + chr1.4:24817859-24817878 None:intergenic 40.0%
TCATCCTCCATTTCCTTTCT+TGG - chr1.4:24817852-24817871 MS.gene59170:CDS 40.0%
TCCACCATAAGTTTCCTACT+AGG + chr1.4:24817760-24817779 None:intergenic 40.0%
TCCATTTGCAGGTAGATTGA+GGG - chr1.4:24817729-24817748 MS.gene59170:CDS 40.0%
TCCTAGTAGGAAACTTATGG+TGG - chr1.4:24817756-24817775 MS.gene59170:CDS 40.0%
TTTCGATCCAACGAAACGAT+GGG - chr1.4:24818713-24818732 MS.gene59170:intron 40.0%
! AACATGGTTCCTTTGTTGTC+GGG + chr1.4:24819836-24819855 None:intergenic 40.0%
! CTAAGCTTTGGTTGCAAGTT+TGG + chr1.4:24818571-24818590 None:intergenic 40.0%
! CTTTATTTGGGAGTTTGGAG+GGG - chr1.4:24818031-24818050 MS.gene59170:CDS 40.0%
! GCATTCAGTGGCAGATTTTA+TGG - chr1.4:24820216-24820235 MS.gene59170:intron 40.0%
!! ATGGTGGATTGTAATGGTGA+AGG - chr1.4:24817772-24817791 MS.gene59170:CDS 40.0%
!! CTGTCTCTAGGGCTTTATTT+GGG - chr1.4:24818019-24818038 MS.gene59170:CDS 40.0%
!! GCCTAGCGAATTGTTTCTAT+TGG - chr1.4:24818211-24818230 MS.gene59170:CDS 40.0%
!! GCTTTATTTGGGAGTTTGGA+GGG - chr1.4:24818030-24818049 MS.gene59170:CDS 40.0%
!! TAGGGCTTTATTTGGGAGTT+TGG - chr1.4:24818026-24818045 MS.gene59170:CDS 40.0%
!!! GAGATGTTGATTTTGGTTGG+GGG - chr1.4:24820311-24820330 MS.gene59170:CDS 40.0%
!!! TGAGTACATGTCACTTTTGG+TGG + chr1.4:24819794-24819813 None:intergenic 40.0%
AACCTCTCCATGACTTGAGT+TGG + chr1.4:24820457-24820476 None:intergenic 45.0%
AACTCAAGTCATGGAGAGGT+TGG - chr1.4:24820456-24820475 MS.gene59170:CDS 45.0%
AATCCGGCAGTAGTTAAAGG+AGG + chr1.4:24820256-24820275 None:intergenic 45.0%
ACACTTTCACGCTGTCTCTA+GGG - chr1.4:24818008-24818027 MS.gene59170:CDS 45.0%
CAATCAACCCATCGTTTCGT+TGG + chr1.4:24818723-24818742 None:intergenic 45.0%
CAGAGAGCTTCTTAACGCAA+GGG - chr1.4:24819766-24819785 MS.gene59170:intron 45.0%
CATGTCGTCTAAGGGAAACA+TGG + chr1.4:24819852-24819871 None:intergenic 45.0%
CATGTTTCCCTTAGACGACA+TGG - chr1.4:24819850-24819869 MS.gene59170:intron 45.0%
CTTTGGTTGCAAGTTTGGAG+GGG + chr1.4:24818566-24818585 None:intergenic 45.0%
GCTTTGGTTGCAAGTTTGGA+GGG + chr1.4:24818567-24818586 None:intergenic 45.0%
GTTTATGGAGGACCAGCTAA+AGG - chr1.4:24820340-24820359 MS.gene59170:CDS 45.0%
TCCCTCAATCTACCTGCAAA+TGG + chr1.4:24817733-24817752 None:intergenic 45.0%
TGGGACCAATGAAGAAAGTG+TGG + chr1.4:24819880-24819899 None:intergenic 45.0%
TGGTGAACCATGTCGTCTAA+GGG + chr1.4:24819860-24819879 None:intergenic 45.0%
TTTGCAGGTAGATTGAGGGA+AGG - chr1.4:24817733-24817752 MS.gene59170:CDS 45.0%
TTTGGTTGCAAGTTTGGAGG+GGG + chr1.4:24818565-24818584 None:intergenic 45.0%
! AGCTTTGGTTGCAAGTTTGG+AGG + chr1.4:24818568-24818587 None:intergenic 45.0%
! CACTCCATTGCTACTTATCC+AGG - chr1.4:24817918-24817937 MS.gene59170:CDS 45.0%
! GGAGAGGTTGGTTAAAGAGT+TGG - chr1.4:24820468-24820487 MS.gene59170:CDS 45.0%
! GTTGCAATTGGTGAGTTGGA+AGG + chr1.4:24819928-24819947 None:intergenic 45.0%
!! GCTGTCTCTAGGGCTTTATT+TGG - chr1.4:24818018-24818037 MS.gene59170:CDS 45.0%
!! GGCTTTATTTGGGAGTTTGG+AGG - chr1.4:24818029-24818048 MS.gene59170:CDS 45.0%
!!! AAATAAAATATATTTTTACA+AGG - chr1.4:24819127-24819146 MS.gene59170:intron 5.0%
!!! ATTTCATTTATAAATTTTTT+TGG + chr1.4:24818661-24818680 None:intergenic 5.0%
!!! ATTTTTTTTCATTTAATTTT+AGG - chr1.4:24818816-24818835 MS.gene59170:intron 5.0%
AACCTTCAATCACACCGGTG+TGG - chr1.4:24819741-24819760 MS.gene59170:intron 50.0%
AGAGCGAATTGCAGCAACCT+CGG + chr1.4:24819903-24819922 None:intergenic 50.0%
AGCGTTATTCACAGCGAAGC+AGG + chr1.4:24818336-24818355 None:intergenic 50.0%
ATGGAGGACCAGCTAAAGGA+GGG - chr1.4:24820344-24820363 MS.gene59170:CDS 50.0%
ATTTGGGAGTTTGGAGGGGA+GGG - chr1.4:24818035-24818054 MS.gene59170:CDS 50.0%
CACACTTTCACGCTGTCTCT+AGG - chr1.4:24818007-24818026 MS.gene59170:CDS 50.0%
CAGGTAACACGCTTCAAGTG+TGG - chr1.4:24819617-24819636 MS.gene59170:intron 50.0%
CCTTAGGAGAGATATCTCGC+GGG - chr1.4:24819711-24819730 MS.gene59170:intron 50.0%
CGCTTTAGCATTTCCCGTTG+CGG - chr1.4:24820102-24820121 MS.gene59170:intron 50.0%
CGTCCTCCTTTAACTACTGC+CGG - chr1.4:24820250-24820269 MS.gene59170:intron 50.0%
CTTGAAGCGTGTTACCTGCA+CGG + chr1.4:24819615-24819634 None:intergenic 50.0%
GAACCTGAACCCGACAACAA+AGG - chr1.4:24819824-24819843 MS.gene59170:intron 50.0%
GATTGAGGGAAGGTCCTAGT+AGG - chr1.4:24817743-24817762 MS.gene59170:CDS 50.0%
GCAGAGAGCTTCTTAACGCA+AGG - chr1.4:24819765-24819784 MS.gene59170:intron 50.0%
GCCTTAGGAGAGATATCTCG+CGG - chr1.4:24819710-24819729 MS.gene59170:intron 50.0%
GGTTGGGGGAAAGCTGTTTA+TGG - chr1.4:24820325-24820344 MS.gene59170:CDS 50.0%
GTAACACGCTTCAAGTGTGG+TGG - chr1.4:24819620-24819639 MS.gene59170:intron 50.0%
GTGGTGAACCATGTCGTCTA+AGG + chr1.4:24819861-24819880 None:intergenic 50.0%
TATGGAGGACCAGCTAAAGG+AGG - chr1.4:24820343-24820362 MS.gene59170:CDS 50.0%
TATTTGGGAGTTTGGAGGGG+AGG - chr1.4:24818034-24818053 MS.gene59170:CDS 50.0%
TCCTGCAGTTGTAACCGCAA+CGG + chr1.4:24820119-24820138 None:intergenic 50.0%
TGCCAACTCAAGTCATGGAG+AGG - chr1.4:24820452-24820471 MS.gene59170:CDS 50.0%
TGGGGGAAAGCTGTTTATGG+AGG - chr1.4:24820328-24820347 MS.gene59170:CDS 50.0%
TGTGATCACCCTTAGTAGGC+TGG + chr1.4:24820519-24820538 None:intergenic 50.0%
TTGAAGCGTGTTACCTGCAC+GGG + chr1.4:24819614-24819633 None:intergenic 50.0%
TTGCAAGTTTGGAGGGGGAA+GGG + chr1.4:24818560-24818579 None:intergenic 50.0%
TTTCTTCATTGGTCCCACCG+AGG - chr1.4:24819883-24819902 MS.gene59170:intron 50.0%
! GTTCCTTTGTTGTCGGGTTC+AGG + chr1.4:24819830-24819849 None:intergenic 50.0%
AAGTTTGGAGGGGGAAGGGA+GGG + chr1.4:24818556-24818575 None:intergenic 55.0%
AGAAGCTCTCTGCTCCACAC+CGG + chr1.4:24819758-24819777 None:intergenic 55.0%
CAATTCGCTAGGCATTCGCG+AGG + chr1.4:24818204-24818223 None:intergenic 55.0%
CCCGCGAGATATCTCTCCTA+AGG + chr1.4:24819714-24819733 None:intergenic 55.0%
CCTGCAGTTGTAACCGCAAC+GGG + chr1.4:24820118-24820137 None:intergenic 55.0%
CGAATTGCAGCAACCTCGGT+GGG + chr1.4:24819899-24819918 None:intergenic 55.0%
CTCCACACCGGTGTGATTGA+AGG + chr1.4:24819746-24819765 None:intergenic 55.0%
GAGGGGAAGAGAGAGCTTTG+TGG - chr1.4:24818053-24818072 MS.gene59170:CDS 55.0%
GTGTCTGATGTTACGCGTGC+TGG - chr1.4:24820283-24820302 MS.gene59170:intron 55.0%
GTTGCAAGTTTGGAGGGGGA+AGG + chr1.4:24818561-24818580 None:intergenic 55.0%
TGGAGGACCAGCTAAAGGAG+GGG - chr1.4:24820345-24820364 MS.gene59170:CDS 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr1.4:24818036-24818055 MS.gene59170:CDS 55.0%
! GGGAAGGGAGGGGATAGTTT+TGG + chr1.4:24818545-24818564 None:intergenic 55.0%
! TTAGGAGGGGAGGGCTTTAG+AGG + chr1.4:24818485-24818504 None:intergenic 55.0%
!! TAGGAGGGGAGGGCTTTAGA+GGG + chr1.4:24818484-24818503 None:intergenic 55.0%
AGTTTGGAGGGGGAAGGGAG+GGG + chr1.4:24818555-24818574 None:intergenic 60.0%
CAAGTTTGGAGGGGGAAGGG+AGG + chr1.4:24818557-24818576 None:intergenic 60.0%
CCCGTTGCGGTTACAACTGC+AGG - chr1.4:24820115-24820134 MS.gene59170:intron 60.0%
GCACCATCCCCTCCTTTAGC+TGG + chr1.4:24820355-24820374 None:intergenic 60.0%
GCGAATTGCAGCAACCTCGG+TGG + chr1.4:24819900-24819919 None:intergenic 60.0%
GGACCAGCTAAAGGAGGGGA+TGG - chr1.4:24820349-24820368 MS.gene59170:CDS 60.0%
GGAGGGGATGGTGCTCTTCC+TGG - chr1.4:24820361-24820380 MS.gene59170:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 24817664 24820567 24817664 ID=MS.gene59170
chr1.4 mRNA 24817664 24820567 24817664 ID=MS.gene59170.t1;Parent=MS.gene59170
chr1.4 exon 24820292 24820567 24820292 ID=MS.gene59170.t1.exon1;Parent=MS.gene59170.t1
chr1.4 CDS 24820292 24820567 24820292 ID=cds.MS.gene59170.t1;Parent=MS.gene59170.t1
chr1.4 exon 24817664 24818611 24817664 ID=MS.gene59170.t1.exon2;Parent=MS.gene59170.t1
chr1.4 CDS 24817664 24818611 24817664 ID=cds.MS.gene59170.t1;Parent=MS.gene59170.t1
Gene Sequence

>MS.gene59170

ATGGCAGGGAAAGATCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGATTGAGGGAAGGTCCTAGTAGGAAACTTATGGTGGATTGTAATGGTGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATGTTACTCTTAAAGAATTTGGTGATGCTCTTCATCCTCCATTTCCTTTCTTGGATGAGCTTCTTTATGATGTTCCTGGTTCTTCAGATGTGATTAACACTCCATTGCTACTTATCCAGGTATAGTATTGATCTTTTCACACTACATATTATCGGAGAAATTTGGATTCTAGTCAGTCACGATTTTCACACTTTCACGCTGTCTCTAGGGCTTTATTTGGGAGTTTGGAGGGGAGGGGAAGAGAGAGCTTTGTGGAAAAATATAAAGGGAAATAGAGAAATCTCTCACATTTTTTCAAAAGAATATTTTTTTAGAGAAAGAGAAACTAATGCTTATAATTCATATATTTTTAATATTAAGAATACGAAAATGCTTCTTCTCCTCGCGAATGCCTAGCGAATTGTTTCTATTGGTTGGTCAAACTTCAACACTCCCCATGTTTTCCTATAATAATGGAACATAAATCCTATAATAGCGAATTGTTTCTCTTCCTTAAAAAATTGAACATGTGACCTGCTTCGCTGTGAATAACGCTGCTCTTAAGAATATTATAACATAGATTTAATTTAAAGAATTAATATAAACCCTTCAAAACTTTTCTTCAATACAACTTCTTAGTTCCCTAAATGAGGATAATTTTGTATTATGAAGAAAAATTAACCCTCTAAAGCCCTCCCCTCCTAATGTTATCTATTTCTTCCACTTGCTCCTTTCCTTTTTTCCAAAACTATCCCCTCCCTTCCCCCTCCAAACTTGCAACCAAAGCTTAGGTGAACATTTGTATCGAGATCACACGGTTCATATTTCAAAATCAAAATAAATAAAAAATCAACTTTTCCAAAAAAATTTATAAATGAAATTCCAAATCTGCATTGAATTTTGACTCCTTTGATTTCGATCCAACGAAACGATGGGTTGATTGCGTGATTGTATGAAAATCGTGACAAAGTCATCTAATTTTTATTTTTTTATTTGATTTTAAGTCTCTAAAATATATTTTTTTTCATTTAATTTTAGGCCATATAGAAAATAAGGTTTTTTCCTCAAAAAAAAAAAAAAAAGGTTTAAATATAGTTTTGTTACTTGTAAGTATACAATTTTTTTTTGTTTGTTTTAGTCATTAGAAGATTTGTTAATGTCAAAATTAGTCCCTGTAAAATGTGTAATTTTCATTTTAGTCCTTACATAAGTATTGAAAGGACTGAAAACTAAACATTTTACATTTTACATGATCAATTCCAACATAAAATATTTTATTATGACTGAATTCTAATGTAAAGCAATTTATTTTATCTTTTCAAGGTCTAAATAAAATATATTTTTACAAGGTTGAAAAATAGAAAGTATTATATTTGTATGCCAAAAAGATATTAAAACCTAGAAGTTAAAATGAATTGAAGCCTTATCGGAATACTACCAAACTTTTAAATACTCTTGATGTGTTATCATAGAAGTTGTTGCCAAAATGATCTCAAAATTTGATTTTTAGGTCTATATTTTAGTTTCTTGAGTTTTCGGAGCTTAAATCTTTTATGATTAATCCTTCACTTATTAAAATAATTTAAACTTTTTGTGAGGAGTTGTTTATAATGTTCTAAACATTCCTTAAAAAAAATGATTCAAATATATGTGGTACCAATTAATGACCATTTTTTACACCACATAGATTTGAACTTTCTACCTTGATGTTACATTGCTATGTTTTTCAAACTAAGCAAGAACCTCAAGTTTAATCACCGCATATCAATTGATTTTAATAAATCTTTAATCATTCATTAATTCATTTCTAACCCGTGCAGGTAACACGCTTCAAGTGTGGTGGTTTCATCTTTGCTCTTCGTATGAACCATACAATGAGTGATGCATCAGGTATGCTTCAATTTTTGAGTGCCTTAGGAGAGATATCTCGCGGGATGAGTAAACCTTCAATCACACCGGTGTGGAGCAGAGAGCTTCTTAACGCAAGGGATCCACCAAAAGTGACATGTACTCATCATGAATACGAACCTGAACCCGACAACAAAGGAACCATGTTTCCCTTAGACGACATGGTTCACCACACTTTCTTCATTGGTCCCACCGAGGTTGCTGCAATTCGCTCTCTCCTTCCAACTCACCAATTGCAACAATACTCCAATTTTGAAATCATTGCTGCATACTTTTGGCGTTGTCGTACAATAGCATTACAACTAGATTCTAACGAAGAGGTTCGCATAATCTGCATAGCCGATGCACGTAGCAAATATGTTGACCAACAACTTCCAAATGGTTACTATGGTAACGCTTTAGCATTTCCCGTTGCGGTTACAACTGCAGGAAAACTTATTGAAAATCCATTGGAGTATGCATTGAATTTAGTTAAGAAAGCAAAAGCTAATGTCAACAAAGAGTATATGCATTCAGTGGCAGATTTTATGGTTATCAAGGGTCGTCCTCCTTTAACTACTGCCGGATTATATTTAGTGTCTGATGTTACGCGTGCTGGATTTAGAGATGTTGATTTTGGTTGGGGGAAAGCTGTTTATGGAGGACCAGCTAAAGGAGGGGATGGTGCTCTTCCTGGTCAAGCTGTCTTTCATATTCCTTTTACAAATGATAAAGGAGAGAAAGGTTTGGTTATACCAGTTTTTTTGCCAACTCAAGTCATGGAGAGGTTGGTTAAAGAGTTGGATAGTGTTCTTAAGAACAACATTAACCAGCCTACTAAGGGTGATCACAAATTTGGCTTCATTAAATCTTCGTTGTAG
Protein sequence

>MS.gene59170.t1

MAGKDPVDIIRKALAKTLVFYYPFAGRLREGPSRKLMVDCNGEGVLFIEADADVTLKEFGDALHPPFPFLDELLYDVPGSSDVINTPLLLIQVTRFKCGGFIFALRMNHTMSDASGMLQFLSALGEISRGMSKPSITPVWSRELLNARDPPKVTCTHHEYEPEPDNKGTMFPLDDMVHHTFFIGPTEVAAIRSLLPTHQLQQYSNFEIIAAYFWRCRTIALQLDSNEEVRIICIADARSKYVDQQLPNGYYGNALAFPVAVTTAGKLIENPLEYALNLVKKAKANVNKEYMHSVADFMVIKGRPPLTTAGLYLVSDVTRAGFRDVDFGWGKAVYGGPAKGGDGALPGQAVFHIPFTNDKGEKGLVIPVFLPTQVMERLVKELDSVLKNNINQPTKGDHKFGFIKSSL