AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59174

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59174.t1 MTR_1g040195 84.434 424 43 1 1 424 1 401 0.0 741
MS.gene59174.t1 MTR_1g040215 75.352 426 103 2 1 424 1 426 0.0 673
MS.gene59174.t1 MTR_1g040175 74.648 426 106 2 1 424 1 426 0.0 664
MS.gene59174.t1 MTR_1g040315 71.294 425 78 4 1 424 1 382 0.0 603
MS.gene59174.t1 MTR_1g040320 69.412 425 94 2 1 424 1 390 0.0 601
MS.gene59174.t1 MTR_7g114550 58.879 428 168 5 2 424 3 427 0.0 530
MS.gene59174.t1 MTR_5g076700 59.811 423 165 3 5 423 6 427 0.0 528
MS.gene59174.t1 MTR_7g114560 60.000 425 162 5 5 424 6 427 0.0 527
MS.gene59174.t1 MTR_7g114490 59.811 423 162 5 5 422 6 425 0.0 524
MS.gene59174.t1 MTR_7g114470 59.434 424 166 3 5 423 7 429 0.0 523
MS.gene59174.t1 MTR_7g114520 60.000 420 160 5 5 418 7 424 0.0 519
MS.gene59174.t1 MTR_1g081660 60.377 424 163 5 4 424 13 434 0.0 513
MS.gene59174.t1 MTR_0042s0190 67.939 262 82 2 165 424 1 262 2.30e-128 372
MS.gene59174.t1 MTR_7g114510 55.517 290 110 5 146 424 7 288 4.39e-104 311
MS.gene59174.t1 MTR_0502s0010 57.438 242 93 5 100 335 1 238 3.33e-94 283
MS.gene59174.t1 MTR_0042s0190 68.528 197 60 2 230 424 1 197 1.48e-93 281
MS.gene59174.t1 MTR_0042s0230 80.272 147 29 0 1 147 1 147 1.54e-82 250
MS.gene59174.t1 MTR_7g114420 49.810 263 100 6 165 423 1 235 1.92e-80 249
MS.gene59174.t1 MTR_8g039950 90.698 129 12 0 1 129 1 129 9.41e-80 242
MS.gene59174.t1 MTR_8g096530 34.761 397 245 7 1 391 1 389 3.07e-77 247
MS.gene59174.t1 MTR_3g035000 56.889 225 70 4 195 417 39 238 2.47e-76 240
MS.gene59174.t1 MTR_3g098980 32.304 421 266 8 9 423 14 421 7.28e-75 240
MS.gene59174.t1 MTR_7g027175 34.625 387 239 7 12 391 19 398 3.16e-73 236
MS.gene59174.t1 MTR_7g114500 60.667 150 59 0 2 151 3 152 3.61e-63 201
MS.gene59174.t1 MTR_3g112180 34.190 389 232 11 19 394 22 399 4.26e-57 194
MS.gene59174.t1 MTR_3g051790 59.589 146 59 0 2 147 6 151 4.91e-56 183
MS.gene59174.t1 MTR_4g055170 30.179 391 245 10 16 385 19 402 3.06e-53 184
MS.gene59174.t1 MTR_1g053315 30.986 426 270 14 17 423 26 446 4.55e-52 181
MS.gene59174.t1 MTR_3g071320 31.266 387 248 9 17 389 13 395 1.39e-49 174
MS.gene59174.t1 MTR_2g105330 28.736 435 273 12 5 420 1 417 1.56e-47 168
MS.gene59174.t1 MTR_4g007540 29.224 438 268 15 5 420 1 418 2.22e-47 168
MS.gene59174.t1 MTR_2g064495 28.506 435 274 12 5 420 1 417 3.25e-46 165
MS.gene59174.t1 MTR_8g075610 29.880 415 269 12 17 420 2 405 5.70e-46 164
MS.gene59174.t1 MTR_7g110610 28.395 405 226 11 5 401 1 349 6.17e-43 155
MS.gene59174.t1 MTR_7g085930 29.792 433 236 14 9 390 6 421 1.31e-41 153
MS.gene59174.t1 MTR_7g084940 27.273 385 257 9 17 389 24 397 6.59e-41 151
MS.gene59174.t1 MTR_2g015430 31.250 368 240 8 30 391 25 385 1.73e-40 150
MS.gene59174.t1 MTR_7g085990 28.868 433 240 13 9 390 2 417 6.84e-39 146
MS.gene59174.t1 MTR_7g084910 26.424 439 274 14 1 423 11 416 4.12e-37 140
MS.gene59174.t1 MTR_4g127650 28.606 409 247 14 17 391 18 415 7.26e-37 140
MS.gene59174.t1 MTR_3g026950 25.971 412 263 11 13 390 6 409 2.97e-36 138
MS.gene59174.t1 MTR_6g053810 30.286 350 216 13 57 385 64 406 8.88e-36 137
MS.gene59174.t1 MTR_4g097090 28.049 410 250 14 16 391 16 414 7.24e-35 134
MS.gene59174.t1 MTR_3g465930 27.404 416 275 12 16 408 17 428 1.85e-34 134
MS.gene59174.t1 MTR_7g086000 27.136 398 241 16 61 422 57 441 4.18e-34 132
MS.gene59174.t1 MTR_3g465990 27.047 403 253 14 16 390 17 406 2.34e-32 127
MS.gene59174.t1 MTR_3g465910 26.866 402 256 13 16 390 17 407 7.33e-32 127
MS.gene59174.t1 MTR_4g088765 68.750 80 23 1 1 78 1 80 1.20e-30 114
MS.gene59174.t1 MTR_4g013050 26.316 399 237 11 12 390 7 368 1.32e-30 122
MS.gene59174.t1 MTR_3g465980 26.651 424 264 15 16 408 17 424 3.11e-30 122
MS.gene59174.t1 MTR_7g056087 26.097 433 274 14 17 423 12 424 6.70e-30 120
MS.gene59174.t1 MTR_3g049150 24.818 411 273 11 6 388 1 403 6.44e-28 115
MS.gene59174.t1 MTR_3g465880 29.240 342 212 12 16 335 17 350 5.07e-27 112
MS.gene59174.t1 MTR_3g465950 25.587 383 249 11 16 376 17 385 5.61e-27 112
MS.gene59174.t1 MTR_5g084870 21.986 423 283 14 15 417 9 404 7.62e-25 106
MS.gene59174.t1 MTR_5g090830 25.109 458 270 17 7 423 8 433 1.71e-24 105
MS.gene59174.t1 MTR_5g090810 24.891 458 271 17 7 423 8 433 3.31e-24 104
MS.gene59174.t1 MTR_3g101930 29.050 358 208 15 9 334 7 350 4.58e-24 104
MS.gene59174.t1 MTR_5g090880 24.938 401 234 13 61 423 64 435 1.36e-23 102
MS.gene59174.t1 MTR_3g450710 26.316 399 224 15 64 423 65 432 3.16e-23 102
MS.gene59174.t1 MTR_3g101960 28.840 319 202 12 23 327 30 337 3.67e-23 101
MS.gene59174.t1 MTR_3g450690 25.888 394 229 14 64 423 72 436 7.07e-23 100
MS.gene59174.t1 MTR_8g079950 22.300 426 284 12 15 417 9 410 1.85e-21 96.7
MS.gene59174.t1 MTR_5g084850 22.308 390 263 12 15 386 9 376 2.05e-21 96.3
MS.gene59174.t1 MTR_8g041770 25.686 401 264 13 14 399 22 403 2.77e-21 95.9
MS.gene59174.t1 MTR_3g101970 28.662 314 196 12 23 327 30 324 4.19e-21 95.5
MS.gene59174.t1 MTR_0190s0020 27.189 434 244 20 23 423 30 424 5.48e-21 95.1
MS.gene59174.t1 MTR_3g102020 27.744 328 193 13 24 327 31 338 9.96e-21 94.4
MS.gene59174.t1 MTR_4g022290 24.505 404 253 13 23 401 31 407 1.71e-20 93.6
MS.gene59174.t1 MTR_8g079970 22.108 389 272 11 15 386 9 383 1.94e-20 93.2
MS.gene59174.t1 MTR_3g102010 25.783 415 242 15 24 399 32 419 2.13e-20 93.2
MS.gene59174.t1 MTR_3g101900 24.424 434 261 15 9 399 7 416 2.17e-20 93.2
MS.gene59174.t1 MTR_5g026320 26.000 400 252 14 22 395 33 414 7.08e-20 91.7
MS.gene59174.t1 MTR_4g094838 24.057 424 259 15 23 422 29 413 7.23e-20 91.7
MS.gene59174.t1 MTR_4g022800 25.754 431 259 20 23 423 25 424 7.28e-20 91.7
MS.gene59174.t1 MTR_7g026860 26.303 422 264 17 23 423 25 420 8.32e-20 91.7
MS.gene59174.t1 MTR_4g094835 24.750 400 257 16 46 422 49 427 1.14e-19 91.3
MS.gene59174.t1 MTR_4g094842 24.612 451 280 20 5 422 6 429 2.26e-19 90.1
MS.gene59174.t1 MTR_3g088420 25.986 431 249 17 23 423 25 415 6.48e-19 89.0
MS.gene59174.t1 MTR_4g094832 26.286 350 214 14 94 422 85 411 1.06e-18 88.2
MS.gene59174.t1 MTR_3g465870 31.579 190 120 4 16 197 19 206 1.11e-18 88.2
MS.gene59174.t1 MTR_1g094790 22.434 419 279 14 12 401 6 407 6.74e-18 85.9
MS.gene59174.t1 MTR_4g022670 25.116 430 262 18 23 423 25 423 8.50e-18 85.5
MS.gene59174.t1 MTR_4g022720 24.561 399 262 14 23 400 25 405 9.46e-18 85.1
MS.gene59174.t1 MTR_5g084900 22.253 364 228 13 64 417 9 327 3.42e-17 82.8
MS.gene59174.t1 MTR_4g022825 25.183 409 239 17 23 394 25 403 3.07e-16 80.9
MS.gene59174.t1 MTR_8g467590 23.844 411 242 22 15 386 9 387 7.51e-16 79.7
MS.gene59174.t1 MTR_7g114417 56.364 55 23 1 89 142 29 83 2.11e-13 65.9
MS.gene59174.t1 MTR_3g049140 28.387 155 93 4 250 388 48 200 7.63e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59174.t1 AT5G17540 59.674 429 163 8 1 423 1 425 1.02e-176 501
MS.gene59174.t1 AT3G03480 57.377 427 167 11 5 423 15 434 6.40e-162 464
MS.gene59174.t1 AT5G41040 34.694 392 236 9 9 389 17 399 6.16e-77 246
MS.gene59174.t1 AT5G63560 34.761 397 239 9 1 389 1 385 7.60e-77 245
MS.gene59174.t1 AT5G41040 34.694 392 236 9 9 389 33 415 7.72e-77 246
MS.gene59174.t1 AT3G48720 31.818 396 258 8 1 390 1 390 2.13e-72 234
MS.gene59174.t1 AT3G62160 32.381 420 251 12 5 411 1 400 5.04e-56 191
MS.gene59174.t1 AT1G27620 31.266 403 240 9 10 394 10 393 7.84e-55 188
MS.gene59174.t1 AT2G40230 33.333 387 234 11 17 388 13 390 1.11e-50 177
MS.gene59174.t1 AT2G19070 29.078 423 249 12 35 422 29 435 1.21e-46 166
MS.gene59174.t1 AT1G28680 30.390 385 252 11 16 391 11 388 3.74e-46 165
MS.gene59174.t1 AT1G03390 30.858 431 275 13 7 423 23 444 3.47e-44 160
MS.gene59174.t1 AT1G65450 29.064 406 259 11 1 389 12 405 1.06e-43 159
MS.gene59174.t1 AT1G28680 31.287 342 225 9 16 349 11 350 2.63e-43 156
MS.gene59174.t1 AT5G57840 27.778 432 266 11 17 422 11 422 4.03e-43 157
MS.gene59174.t1 AT5G48930 27.273 418 280 9 17 420 11 418 6.43e-42 153
MS.gene59174.t1 AT5G07080 30.147 408 240 13 7 385 12 403 1.60e-41 153
MS.gene59174.t1 AT3G47170 28.767 365 210 13 7 335 10 360 2.31e-39 147
MS.gene59174.t1 AT2G23510 28.261 414 248 14 1 385 5 398 8.94e-35 134
MS.gene59174.t1 AT2G25150 29.293 396 253 14 15 391 19 406 1.97e-34 134
MS.gene59174.t1 AT1G78990 29.014 355 221 11 62 395 65 409 2.43e-31 125
MS.gene59174.t1 AT1G31490 26.161 409 250 14 6 387 3 386 4.85e-31 124
MS.gene59174.t1 AT1G32910 26.425 386 232 14 62 423 63 420 4.04e-26 110
MS.gene59174.t1 AT3G26040 24.121 398 255 15 15 388 9 383 1.15e-24 105
MS.gene59174.t1 AT3G50270 26.905 420 236 17 23 401 30 419 1.29e-24 105
MS.gene59174.t1 AT5G07860 24.385 447 284 13 4 423 12 431 5.54e-24 103
MS.gene59174.t1 AT5G42830 27.078 421 245 14 15 399 23 417 2.38e-23 102
MS.gene59174.t1 AT5G07850 27.335 439 247 19 4 399 12 421 6.57e-23 100
MS.gene59174.t1 AT4G15390 24.091 440 286 15 3 417 2 418 1.25e-22 100
MS.gene59174.t1 AT4G15390 24.091 440 286 15 3 417 2 418 1.25e-22 100
MS.gene59174.t1 AT5G07870 26.446 363 235 11 64 399 72 429 2.63e-22 99.4
MS.gene59174.t1 AT4G31910 26.866 402 261 13 48 422 48 443 6.40e-22 97.8
MS.gene59174.t1 AT5G16410 26.648 349 219 13 62 388 89 422 6.49e-22 98.2
MS.gene59174.t1 AT1G24420 26.566 399 237 17 15 386 11 380 1.35e-21 96.7
MS.gene59174.t1 AT1G24420 26.566 399 237 17 15 386 11 380 1.35e-21 96.7
MS.gene59174.t1 AT3G50280 26.010 396 252 15 23 395 28 405 2.47e-21 96.3
MS.gene59174.t1 AT1G65450 28.226 248 156 6 157 389 1 241 3.37e-21 93.6
MS.gene59174.t1 AT3G50300 26.685 356 222 15 64 392 59 402 1.27e-20 94.0
MS.gene59174.t1 AT1G24430 24.566 403 251 16 12 390 8 381 4.73e-20 92.4
MS.gene59174.t1 AT5G23970 24.419 430 273 14 13 423 7 403 4.19e-18 86.3
MS.gene59174.t1 AT5G67160 25.806 372 244 15 58 408 50 410 9.94e-17 82.4
MS.gene59174.t1 AT5G47950 23.394 436 264 17 9 417 6 398 2.92e-14 74.7
MS.gene59174.t1 AT5G23940 24.752 404 211 20 41 383 28 399 3.03e-13 71.6
MS.gene59174.t1 AT3G50280 24.176 273 189 9 136 395 8 275 7.91e-13 69.3
MS.gene59174.t1 AT3G50290 25.000 224 134 8 138 342 16 224 1.39e-11 65.1

Find 89 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTGATGATTTCGAATT+TGG 0.114001 1.4:+24868016 None:intergenic
GGGTCTTTCCCTTCCATATT+TGG 0.169165 1.4:+24870791 None:intergenic
ATCCATTTGCAGGTAGATTA+AGG 0.198110 1.4:-24870721 MS.gene59174:CDS
GCATTCAGTTGCGGATTTAA+TGG 0.222413 1.4:-24867757 MS.gene59174:CDS
TTTGAGGGTCTTACAAATTC+AGG 0.230264 1.4:+24870908 None:intergenic
TTTGAAAACGTAACCCTTCT+TGG 0.271683 1.4:+24870843 None:intergenic
GGACTTAGAGAGGTTGATTT+TGG 0.303724 1.4:-24867669 MS.gene59174:CDS
CACCTACAGCACTCATGAAT+TGG 0.305227 1.4:+24868270 None:intergenic
TTATAAATATGATCCAAATA+TGG 0.321793 1.4:-24870804 MS.gene59174:CDS
GCATTTCCATAGTAACCTTT+TGG 0.330837 1.4:+24867891 None:intergenic
AAACTAATTGAAAATCCATT+AGG 0.336479 1.4:-24867834 MS.gene59174:CDS
GATGTTAGTTTGAAAGAATT+TGG 0.347283 1.4:-24870629 MS.gene59174:CDS
CCTGCAGTTGTAATTGCAAC+AGG 0.365928 1.4:+24867858 None:intergenic
AACTCAAGTGCATACCCTAA+TGG 0.368393 1.4:+24867819 None:intergenic
TGCTAAAGGAGAGGAAGGTT+TGG 0.383515 1.4:-24867559 MS.gene59174:CDS
AACTAATTGAAAATCCATTA+GGG 0.389241 1.4:-24867833 MS.gene59174:CDS
CCGTTGCCAAAAGGTTACTA+TGG 0.390644 1.4:-24867897 MS.gene59174:CDS
CGAATTTGGATTGTTGTTGT+AGG 0.393458 1.4:+24868030 None:intergenic
TCACTGTGAATAAAAGAGAT+TGG 0.393886 1.4:+24870942 None:intergenic
CATTACACCCTTAGATGAAA+TGG 0.397062 1.4:-24868117 MS.gene59174:CDS
GCGGTGGACCATTTCATCTA+AGG 0.404967 1.4:+24868109 None:intergenic
TCCATTTGCAGGTAGATTAA+GGG 0.410433 1.4:-24870720 MS.gene59174:CDS
AATGAGCGACGCGACGGGTT+TGG 0.422279 1.4:-24868294 MS.gene59174:CDS
TGCGGATTTAATGGTTATCA+AGG 0.430701 1.4:-24867748 MS.gene59174:CDS
AAATCATCACTGCATGCCTT+TGG 0.430886 1.4:-24868007 MS.gene59174:CDS
AGGTCCTGCTAGGAAACTTA+TGG 0.433584 1.4:-24870696 MS.gene59174:CDS
CCTGTTGCAATTACAACTGC+AGG 0.441218 1.4:-24867858 MS.gene59174:CDS
CGAACTTCCTCGTCTGTGTC+TGG 0.443541 1.4:+24867960 None:intergenic
CATTTCATCTAAGGGTGTAA+TGG 0.449505 1.4:+24868118 None:intergenic
TATTTATAAAATTGTATCAC+TGG 0.450663 1.4:+24870818 None:intergenic
CACTCCAATCCTTCTTATCC+AGG 0.451699 1.4:-24870531 MS.gene59174:intron
TCCCTTAATCTACCTGCAAA+TGG 0.452547 1.4:+24870719 None:intergenic
CACTGTGAATAAAAGAGATT+GGG 0.457495 1.4:+24870943 None:intergenic
CTGTAGGTGAAATTTCTTGC+GGG 0.463153 1.4:-24868256 MS.gene59174:CDS
AATAATATACCTGGATAAGA+AGG 0.480629 1.4:+24870522 None:intergenic
TTGAGGGTCTTACAAATTCA+GGG 0.486277 1.4:+24870909 None:intergenic
AACTCATCCAAACAAGGAAA+AGG 0.492878 1.4:+24870590 None:intergenic
AAACTTATGGTGGATTGCAC+TGG 0.500723 1.4:-24870683 MS.gene59174:CDS
TGTAGGTGACGTGGAAATAG+AGG 0.515080 1.4:+24868047 None:intergenic
CCGTCGCGTCGCTCATTGCA+TGG 0.516181 1.4:+24868300 None:intergenic
CGATGAGTGCATGTTACTCT+TGG 0.517477 1.4:+24868173 None:intergenic
TATACCTGGATAAGAAGGAT+TGG 0.520763 1.4:+24870527 None:intergenic
GAACAAGTTCCTGACACAAA+AGG 0.522484 1.4:-24868143 MS.gene59174:CDS
GTATATGGAGGACCAGCTAC+AGG 0.522562 1.4:-24867633 MS.gene59174:CDS
TGCCATACCGGTGAGATTGA+AGG 0.523692 1.4:+24868224 None:intergenic
AACCTCTCCATAGCTTAAGT+TGG 0.530625 1.4:+24867519 None:intergenic
ACACCAACTCCTCCTGTAGC+TGG 0.532688 1.4:+24867621 None:intergenic
TCAGATATAGATGACCAAGA+AGG 0.542060 1.4:-24870857 MS.gene59174:CDS
CAGATATAGATGACCAAGAA+GGG 0.543769 1.4:-24870856 MS.gene59174:CDS
AAATATGATCCAAATATGGA+AGG 0.544870 1.4:-24870800 MS.gene59174:CDS
GTGTCTGATGTTACACATGC+TGG 0.545058 1.4:-24867690 MS.gene59174:CDS
CGGTGGACCATTTCATCTAA+GGG 0.545372 1.4:+24868110 None:intergenic
TGAGTGCATGTTACTCTTGG+TGG 0.546616 1.4:+24868176 None:intergenic
AATATGATCCAAATATGGAA+GGG 0.552554 1.4:-24870799 MS.gene59174:CDS
ATAGAGAGCTTCTAAACGCA+AGG 0.554177 1.4:-24868202 MS.gene59174:CDS
AACCTTCAATCTCACCGGTA+TGG 0.559901 1.4:-24868226 MS.gene59174:CDS
CGATTGTACGGCAACGCCAA+AGG 0.564878 1.4:+24867991 None:intergenic
GGACCAGCTACAGGAGGAGT+TGG 0.569672 1.4:-24867624 MS.gene59174:CDS
CGAAGTGCAGATAACTCAGT+TGG 0.569992 1.4:+24868071 None:intergenic
TCCACCATAAGTTTCCTAGC+AGG 0.571714 1.4:+24870692 None:intergenic
AGAATACATGCATTCAGTTG+CGG 0.574989 1.4:-24867766 MS.gene59174:CDS
CATGCAATGAGCGACGCGAC+GGG 0.578001 1.4:-24868299 MS.gene59174:CDS
CTGGTTGTAATGCGATTGTA+CGG 0.579543 1.4:+24867979 None:intergenic
AGAAGCTCTCTATGCCATAC+CGG 0.587394 1.4:+24868212 None:intergenic
GAATGAACCTTCAATCTCAC+CGG 0.587572 1.4:-24868231 MS.gene59174:CDS
GATTAAGGGAAGGTCCTGCT+AGG 0.590882 1.4:-24870706 MS.gene59174:CDS
CCATGCAATGAGCGACGCGA+CGG 0.601332 1.4:-24868300 MS.gene59174:CDS
GCTTAAGTTGGCAAACAAAG+TGG 0.603180 1.4:+24867531 None:intergenic
GCGGATTTAATGGTTATCAA+GGG 0.606067 1.4:-24867747 MS.gene59174:CDS
ATATAAAAGCTAGCAACACC+AGG 0.611199 1.4:+24867594 None:intergenic
TCATCCAAACAAGGAAAAGG+AGG 0.613793 1.4:+24870593 None:intergenic
TACACATGCTGGACTTAGAG+AGG 0.617962 1.4:-24867679 MS.gene59174:CDS
GGAGGAGTTGGTGTTACACC+TGG 0.626042 1.4:-24867612 MS.gene59174:CDS
TGAGGGTCTTACAAATTCAG+GGG 0.629933 1.4:+24870910 None:intergenic
TCCTGCTAGGAAACTTATGG+TGG 0.635781 1.4:-24870693 MS.gene59174:CDS
ATTCTCACCACTGTGAAATG+TGG 0.636248 1.4:+24867720 None:intergenic
GTCCAATTCATGAGTGCTGT+AGG 0.638777 1.4:-24868272 MS.gene59174:CDS
AAAAGAAACTCATCCAAACA+AGG 0.642589 1.4:+24870584 None:intergenic
ATGGTGGATTGCACTGGAGA+AGG 0.645652 1.4:-24870677 MS.gene59174:CDS
GATTGTTGTTGTAGGTGACG+TGG 0.649549 1.4:+24868038 None:intergenic
TAGAGAGCTTCTAAACGCAA+GGG 0.655440 1.4:-24868201 MS.gene59174:CDS
GCTGTAGGTGAAATTTCTTG+CGG 0.660670 1.4:-24868257 MS.gene59174:CDS
TTTGCAGGTAGATTAAGGGA+AGG 0.667024 1.4:-24870716 MS.gene59174:CDS
TATGGAGGACCAGCTACAGG+AGG 0.683939 1.4:-24867630 MS.gene59174:CDS
TTAATCACATCTGAATAACC+AGG 0.690578 1.4:+24870554 None:intergenic
GTAACACGTCTCAAGTGTGG+TGG 0.692143 1.4:-24868347 MS.gene59174:CDS
TAGGTAACACGTCTCAAGTG+TGG 0.700766 1.4:-24868350 MS.gene59174:CDS
ATTACAACCAGACACAGACG+AGG 0.718438 1.4:-24867967 MS.gene59174:CDS
GGGTAGACCACATTTCACAG+TGG 0.791465 1.4:-24867727 MS.gene59174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAAAAATTAATATT+CGG + chr1.4:24868538-24868557 None:intergenic 0.0%
!! ACTAGTTATTTAATTAAAAA+AGG - chr1.4:24868236-24868255 MS.gene59174:CDS 10.0%
!! TTTATCAAAATAAAATTATG+GGG - chr1.4:24869523-24869542 MS.gene59174:intron 10.0%
!!! CAAAATTGAATTTTAAATTA+AGG - chr1.4:24869301-24869320 MS.gene59174:intron 10.0%
!! AAAACTCAAAATACAAAATA+TGG + chr1.4:24868961-24868980 None:intergenic 15.0%
!! TACATAATATATGTAAAGTT+CGG + chr1.4:24868088-24868107 None:intergenic 15.0%
!! TAGTTATTCAAATTTATTAC+TGG - chr1.4:24868380-24868399 MS.gene59174:intron 15.0%
!! TATTTATAAAATTGTATCAC+TGG + chr1.4:24867665-24867684 None:intergenic 15.0%
!! TTATAAATATGATCCAAATA+TGG - chr1.4:24867676-24867695 MS.gene59174:CDS 15.0%
!!! AAGATTTGAGTTAATTAATT+AGG + chr1.4:24869458-24869477 None:intergenic 15.0%
!!! AGTAATGATGTTTTTTATTT+AGG - chr1.4:24868712-24868731 MS.gene59174:intron 15.0%
!!! ATTTTCCTAATCATAATTTT+GGG + chr1.4:24869726-24869745 None:intergenic 15.0%
!!! TATTTTCCTAATCATAATTT+TGG + chr1.4:24869727-24869746 None:intergenic 15.0%
!!! TGCTTATATTTTATTAGAAA+GGG + chr1.4:24868186-24868205 None:intergenic 15.0%
!!! TTGCTTATATTTTATTAGAA+AGG + chr1.4:24868187-24868206 None:intergenic 15.0%
!! AAACTAATTGAAAATCCATT+AGG - chr1.4:24870646-24870665 MS.gene59174:CDS 20.0%
!! AAACTCAAAAGCAAAAAAAT+TGG + chr1.4:24868934-24868953 None:intergenic 20.0%
!! AACTAATTGAAAATCCATTA+GGG - chr1.4:24870647-24870666 MS.gene59174:CDS 20.0%
!! ACATAAAAAATAATGATGGT+GGG - chr1.4:24869604-24869623 MS.gene59174:intron 20.0%
!! ACATAATATATGTAAAGTTC+GGG + chr1.4:24868087-24868106 None:intergenic 20.0%
!! AGACACATAAAAAATAATGA+TGG - chr1.4:24869600-24869619 MS.gene59174:intron 20.0%
!! ATCATAAGAGATATGATATA+TGG + chr1.4:24870067-24870086 None:intergenic 20.0%
!! ATGATTAGGAAAATACTAAA+TGG - chr1.4:24869732-24869751 MS.gene59174:intron 20.0%
!! GAAAATATCAATAATATACC+TGG + chr1.4:24867970-24867989 None:intergenic 20.0%
!! GAAATGGAAAAAGTTATAAA+TGG - chr1.4:24869688-24869707 MS.gene59174:intron 20.0%
!! GAAATGGAAAAAGTTATAAA+TGG + chr1.4:24869707-24869688 None:intergenic 20.0%
!! TATAAATGGATAGCATTTAA+AGG - chr1.4:24868336-24868355 MS.gene59174:CDS 20.0%
!! TCACCACAAAAAAAAAAAAT+CGG + chr1.4:24868051-24868070 None:intergenic 20.0%
!! TTAAAATTCAAGATACGATA+CGG + chr1.4:24869025-24869044 None:intergenic 20.0%
!! TTATGAATTTATGTGAATGA+AGG + chr1.4:24870092-24870111 None:intergenic 20.0%
!! TTCTTGTAAAAGAATAAATC+AGG + chr1.4:24869845-24869864 None:intergenic 20.0%
!! TTGAATATAATTACTTCGAA+TGG + chr1.4:24869119-24869138 None:intergenic 20.0%
!! TTTAACTATATAGATACAAG+TGG - chr1.4:24869265-24869284 MS.gene59174:intron 20.0%
!!! ATATTTTCATGATGTATCAA+AGG - chr1.4:24867982-24868001 MS.gene59174:CDS 20.0%
!!! CAAATGTATTTGAAAAGTAT+CGG + chr1.4:24868568-24868587 None:intergenic 20.0%
!!! CTTTTCTAATAATATCAACA+GGG + chr1.4:24867712-24867731 None:intergenic 20.0%
!!! GAAAGTAATTTTATTTCATG+AGG + chr1.4:24867605-24867624 None:intergenic 20.0%
!!! GATACTTTTCAAATACATTT+GGG - chr1.4:24868567-24868586 MS.gene59174:intron 20.0%
!!! TAATTATATTCAAAGTGTCT+TGG - chr1.4:24869126-24869145 MS.gene59174:intron 20.0%
! AAATATGATCCAAATATGGA+AGG - chr1.4:24867680-24867699 MS.gene59174:CDS 25.0%
! AATAATATACCTGGATAAGA+AGG + chr1.4:24867961-24867980 None:intergenic 25.0%
! AATATGATCCAAATATGGAA+GGG - chr1.4:24867681-24867700 MS.gene59174:CDS 25.0%
! ACAGACATAGAAAAACAATA+AGG + chr1.4:24868894-24868913 None:intergenic 25.0%
! ACTAACAATCCAATACTTAA+TGG - chr1.4:24869933-24869952 MS.gene59174:intron 25.0%
! ATACCTTTCAAAAATGCTAA+AGG - chr1.4:24870907-24870926 MS.gene59174:CDS 25.0%
! ATTACTTAGTCAATTACATC+AGG + chr1.4:24868698-24868717 None:intergenic 25.0%
! ATTGAATTGTAATTGTGTGT+AGG - chr1.4:24870111-24870130 MS.gene59174:intron 25.0%
! CAATCACAAGTGTTTAATTA+TGG - chr1.4:24869485-24869504 MS.gene59174:intron 25.0%
! CACATAAAAAATAATGATGG+TGG - chr1.4:24869603-24869622 MS.gene59174:intron 25.0%
! CATAATATATGTAAAGTTCG+GGG + chr1.4:24868086-24868105 None:intergenic 25.0%
! CATTATCGTATCATATATCA+GGG - chr1.4:24869082-24869101 MS.gene59174:intron 25.0%
! CCAAATATAATAGAAACATC+AGG + chr1.4:24868634-24868653 None:intergenic 25.0%
! CTAACAATCCAATACTTAAT+GGG - chr1.4:24869934-24869953 MS.gene59174:intron 25.0%
! CTTTACCCAAAATTATGATT+AGG - chr1.4:24869718-24869737 MS.gene59174:intron 25.0%
! GATACGATAATGATATGATA+TGG + chr1.4:24869074-24869093 None:intergenic 25.0%
! TAACAATCCAATACTTAATG+GGG - chr1.4:24869935-24869954 MS.gene59174:intron 25.0%
! TATCAAACAAGACAAAACAT+CGG + chr1.4:24869914-24869933 None:intergenic 25.0%
! TCATTATCGTATCATATATC+AGG - chr1.4:24869081-24869100 MS.gene59174:intron 25.0%
! TCGAATAGAATTGCTATAAA+TGG - chr1.4:24868322-24868341 MS.gene59174:CDS 25.0%
! TCGATATTCAAACATTAACT+CGG + chr1.4:24868306-24868325 None:intergenic 25.0%
!! CATAAAAAATAATGATGGTG+GGG - chr1.4:24869605-24869624 MS.gene59174:intron 25.0%
!! CCTGATGTTTCTATTATATT+TGG - chr1.4:24868631-24868650 MS.gene59174:intron 25.0%
!! CGATACTTTTCAAATACATT+TGG - chr1.4:24868566-24868585 MS.gene59174:intron 25.0%
!! GATGTTAGTTTGAAAGAATT+TGG - chr1.4:24867851-24867870 MS.gene59174:CDS 25.0%
!! GCTTTTCTAATAATATCAAC+AGG + chr1.4:24867713-24867732 None:intergenic 25.0%
!! TTTTTCCATTTCTTGCAATT+TGG - chr1.4:24869569-24869588 MS.gene59174:intron 25.0%
!! TTTTTCCATTTCTTGCAATT+TGG + chr1.4:24869588-24869569 None:intergenic 25.0%
!!! AATTTTATTTCATGAGGTGT+TGG + chr1.4:24867599-24867618 None:intergenic 25.0%
!!! ATTGTTTTTCTATGTCTGTT+TGG - chr1.4:24868895-24868914 MS.gene59174:intron 25.0%
!!! CTATTGTTTTATTGTGTGAA+GGG - chr1.4:24868853-24868872 MS.gene59174:intron 25.0%
!!! GTCTTTGATTTTCTTTTTAC+CGG - chr1.4:24869987-24870006 MS.gene59174:intron 25.0%
!!! TCATTCATTTTGAAGTATCT+AGG + chr1.4:24868446-24868465 None:intergenic 25.0%
!!! TTTTTTTTTTTTGTGGTGAC+CGG - chr1.4:24868052-24868071 MS.gene59174:CDS 25.0%
AAAAGAAACTCATCCAAACA+AGG + chr1.4:24867899-24867918 None:intergenic 30.0%
CACTGTGAATAAAAGAGATT+GGG + chr1.4:24867540-24867559 None:intergenic 30.0%
GATAGACACACAAAAATAAG+TGG + chr1.4:24869652-24869671 None:intergenic 30.0%
GGAAAAAGTTATAAATGGAG+AGG - chr1.4:24869693-24869712 MS.gene59174:intron 30.0%
TCACTGTGAATAAAAGAGAT+TGG + chr1.4:24867541-24867560 None:intergenic 30.0%
TTAATCACATCTGAATAACC+AGG + chr1.4:24867929-24867948 None:intergenic 30.0%
TTTCAAAAATGCTAAAGGAG+AGG - chr1.4:24870912-24870931 MS.gene59174:CDS 30.0%
! CAAATACATTTGGGATACAA+GGG - chr1.4:24868576-24868595 MS.gene59174:intron 30.0%
! CTTTTAACCCCATTAAGTAT+TGG + chr1.4:24869945-24869964 None:intergenic 30.0%
! TCAAATACATTTGGGATACA+AGG - chr1.4:24868575-24868594 MS.gene59174:intron 30.0%
! TCTCCTTTAGCATTTTTGAA+AGG + chr1.4:24870913-24870932 None:intergenic 30.0%
!! GCTATTGTTTTATTGTGTGA+AGG - chr1.4:24868852-24868871 MS.gene59174:intron 30.0%
!!! GCACCGATTTTTTTTTTTTG+TGG - chr1.4:24868045-24868064 MS.gene59174:CDS 30.0%
AAAAATGCTAAAGGAGAGGA+AGG - chr1.4:24870916-24870935 MS.gene59174:CDS 35.0%
AACGAAGCAATGTTACAACA+TGG + chr1.4:24869179-24869198 None:intergenic 35.0%
AACTCATCCAAACAAGGAAA+AGG + chr1.4:24867893-24867912 None:intergenic 35.0%
ACATAGAGACACAAAACAGA+CGG + chr1.4:24868817-24868836 None:intergenic 35.0%
AGAATACATGCATTCAGTTG+CGG - chr1.4:24870714-24870733 MS.gene59174:CDS 35.0%
ATATAAAAGCTAGCAACACC+AGG + chr1.4:24870889-24870908 None:intergenic 35.0%
ATCATATATCAGGGCTTCAT+AGG - chr1.4:24869091-24869110 MS.gene59174:intron 35.0%
ATCCATTTGCAGGTAGATTA+AGG - chr1.4:24867759-24867778 MS.gene59174:CDS 35.0%
CAGATATAGATGACCAAGAA+GGG - chr1.4:24867624-24867643 MS.gene59174:CDS 35.0%
CATTACACCCTTAGATGAAA+TGG - chr1.4:24870363-24870382 MS.gene59174:intron 35.0%
GAAACTTGCATGTTGTTCAA+GGG - chr1.4:24870009-24870028 MS.gene59174:intron 35.0%
GACACACAAAAATAAGTGGT+AGG + chr1.4:24869648-24869667 None:intergenic 35.0%
GCGGATTTAATGGTTATCAA+GGG - chr1.4:24870733-24870752 MS.gene59174:CDS 35.0%
TATACCTGGATAAGAAGGAT+TGG + chr1.4:24867956-24867975 None:intergenic 35.0%
TCAGATATAGATGACCAAGA+AGG - chr1.4:24867623-24867642 MS.gene59174:CDS 35.0%
TCCATTTGCAGGTAGATTAA+GGG - chr1.4:24867760-24867779 MS.gene59174:CDS 35.0%
TCTCTCCAAATTGCAAGAAA+TGG - chr1.4:24869672-24869691 MS.gene59174:intron 35.0%
TCTCTCCAAATTGCAAGAAA+TGG + chr1.4:24869691-24869672 None:intergenic 35.0%
TGCAGTGATGATTTCGAATT+TGG + chr1.4:24870467-24870486 None:intergenic 35.0%
TGCGGATTTAATGGTTATCA+AGG - chr1.4:24870732-24870751 MS.gene59174:CDS 35.0%
TTAAAAAAGGTTCAGTCCAG+TGG - chr1.4:24868249-24868268 MS.gene59174:CDS 35.0%
TTGAACAACATGCAAGTTTC+CGG + chr1.4:24870009-24870028 None:intergenic 35.0%
TTGAGGGTCTTACAAATTCA+GGG + chr1.4:24867574-24867593 None:intergenic 35.0%
TTTCTATGTCTGTTTGGTCT+AGG - chr1.4:24868901-24868920 MS.gene59174:intron 35.0%
TTTGAAAACGTAACCCTTCT+TGG + chr1.4:24867640-24867659 None:intergenic 35.0%
TTTGAGGGTCTTACAAATTC+AGG + chr1.4:24867575-24867594 None:intergenic 35.0%
! CATTTCATCTAAGGGTGTAA+TGG + chr1.4:24870365-24870384 None:intergenic 35.0%
! CGAATTTGGATTGTTGTTGT+AGG + chr1.4:24870453-24870472 None:intergenic 35.0%
! GCATTTCCATAGTAACCTTT+TGG + chr1.4:24870592-24870611 None:intergenic 35.0%
! GTGTTTTACTATCCATTTGC+AGG - chr1.4:24867749-24867768 MS.gene59174:CDS 35.0%
!! TTAGAGAGGTTGATTTTGGT+TGG - chr1.4:24870815-24870834 MS.gene59174:CDS 35.0%
!!! TAGAGAGGTTGATTTTGGTT+GGG - chr1.4:24870816-24870835 MS.gene59174:CDS 35.0%
AAATCATCACTGCATGCCTT+TGG - chr1.4:24870473-24870492 MS.gene59174:intron 40.0%
AACTCAAGTGCATACCCTAA+TGG + chr1.4:24870664-24870683 None:intergenic 40.0%
AAGACAAAACATCGGAGAAG+TGG + chr1.4:24869906-24869925 None:intergenic 40.0%
ATAGAGAGCTTCTAAACGCA+AGG - chr1.4:24870278-24870297 MS.gene59174:intron 40.0%
ATCTAGGCTTCATCAGAGAA+TGG + chr1.4:24868430-24868449 None:intergenic 40.0%
ATTCTCACCACTGTGAAATG+TGG + chr1.4:24870763-24870782 None:intergenic 40.0%
CTGGTTGTAATGCGATTGTA+CGG + chr1.4:24870504-24870523 None:intergenic 40.0%
CTGTAGGTGAAATTTCTTGC+GGG - chr1.4:24870224-24870243 MS.gene59174:intron 40.0%
GAACAAGTTCCTGACACAAA+AGG - chr1.4:24870337-24870356 MS.gene59174:intron 40.0%
GAATGAACCTTCAATCTCAC+CGG - chr1.4:24870249-24870268 MS.gene59174:intron 40.0%
GATACTTCTCGATACGTATC+AGG - chr1.4:24868505-24868524 MS.gene59174:intron 40.0%
GATGTTGAGTCTAGTGAAAG+TGG + chr1.4:24869219-24869238 None:intergenic 40.0%
GCATTCAGTTGCGGATTTAA+TGG - chr1.4:24870723-24870742 MS.gene59174:CDS 40.0%
GCTGTAGGTGAAATTTCTTG+CGG - chr1.4:24870223-24870242 MS.gene59174:intron 40.0%
GGAAACTTGCATGTTGTTCA+AGG - chr1.4:24870008-24870027 MS.gene59174:intron 40.0%
TAGAGAGCTTCTAAACGCAA+GGG - chr1.4:24870279-24870298 MS.gene59174:intron 40.0%
TCATCCAAACAAGGAAAAGG+AGG + chr1.4:24867890-24867909 None:intergenic 40.0%
TCCCTTAATCTACCTGCAAA+TGG + chr1.4:24867764-24867783 None:intergenic 40.0%
TGAGGGTCTTACAAATTCAG+GGG + chr1.4:24867573-24867592 None:intergenic 40.0%
TTGGACCGAAGAAAAAAGAG+CGG + chr1.4:24870393-24870412 None:intergenic 40.0%
TTTGCAGGTAGATTAAGGGA+AGG - chr1.4:24867764-24867783 MS.gene59174:CDS 40.0%
! ATAGTAACCTTTTGGCAACG+GGG + chr1.4:24870584-24870603 None:intergenic 40.0%
! CATAGTAACCTTTTGGCAAC+GGG + chr1.4:24870585-24870604 None:intergenic 40.0%
! CCATAGTAACCTTTTGGCAA+CGG + chr1.4:24870586-24870605 None:intergenic 40.0%
! GAGTTTCTTTTCGATGTTCC+TGG - chr1.4:24867908-24867927 MS.gene59174:CDS 40.0%
! GTAATGGTTCCTTTTGTGTC+AGG + chr1.4:24870349-24870368 None:intergenic 40.0%
! GTATCGGTATAGGATACACA+GGG + chr1.4:24868479-24868498 None:intergenic 40.0%
! TATCGAGCATGTATCGGTAT+AGG + chr1.4:24868489-24868508 None:intergenic 40.0%
! TCAACCTCCTTTTCCTTGTT+TGG - chr1.4:24867883-24867902 MS.gene59174:CDS 40.0%
! TGTATCGGTATAGGATACAC+AGG + chr1.4:24868480-24868499 None:intergenic 40.0%
! TTTGGGATACAAGGGATATG+CGG - chr1.4:24868584-24868603 MS.gene59174:intron 40.0%
!! AAACTTATGGTGGATTGCAC+TGG - chr1.4:24867797-24867816 MS.gene59174:CDS 40.0%
!! GAGAAGTATCGAGCATGTAT+CGG + chr1.4:24868495-24868514 None:intergenic 40.0%
!! GGACTTAGAGAGGTTGATTT+TGG - chr1.4:24870811-24870830 MS.gene59174:CDS 40.0%
!!! AGAGAGGTTGATTTTGGTTG+GGG - chr1.4:24870817-24870836 MS.gene59174:CDS 40.0%
!!! TCATGAGGTGTTGGTTTTGA+GGG + chr1.4:24867590-24867609 None:intergenic 40.0%
!!! TTCATGAGGTGTTGGTTTTG+AGG + chr1.4:24867591-24867610 None:intergenic 40.0%
AAAGTTCGGGGTTCGAATTC+CGG + chr1.4:24868074-24868093 None:intergenic 45.0%
AACCTTCAATCTCACCGGTA+TGG - chr1.4:24870254-24870273 MS.gene59174:intron 45.0%
AGAAGCTCTCTATGCCATAC+CGG + chr1.4:24870271-24870290 None:intergenic 45.0%
AGGTCCTGCTAGGAAACTTA+TGG - chr1.4:24867784-24867803 MS.gene59174:CDS 45.0%
ATTACAACCAGACACAGACG+AGG - chr1.4:24870513-24870532 MS.gene59174:intron 45.0%
CACCTACAGCACTCATGAAT+TGG + chr1.4:24870213-24870232 None:intergenic 45.0%
CACTCCAATCCTTCTTATCC+AGG - chr1.4:24867949-24867968 MS.gene59174:CDS 45.0%
CCGTTGCCAAAAGGTTACTA+TGG - chr1.4:24870583-24870602 MS.gene59174:CDS 45.0%
CCTGCAGTTGTAATTGCAAC+AGG + chr1.4:24870625-24870644 None:intergenic 45.0%
CCTGTTGCAATTACAACTGC+AGG - chr1.4:24870622-24870641 MS.gene59174:CDS 45.0%
CGAAGTGCAGATAACTCAGT+TGG + chr1.4:24870412-24870431 None:intergenic 45.0%
CGATGAGTGCATGTTACTCT+TGG + chr1.4:24870310-24870329 None:intergenic 45.0%
CGGTGGACCATTTCATCTAA+GGG + chr1.4:24870373-24870392 None:intergenic 45.0%
GGGTCTTTCCCTTCCATATT+TGG + chr1.4:24867692-24867711 None:intergenic 45.0%
GTGTCTGATGTTACACATGC+TGG - chr1.4:24870790-24870809 MS.gene59174:CDS 45.0%
GTGTTATGTTCTCGTGAGCT+TGG + chr1.4:24868129-24868148 None:intergenic 45.0%
TACACATGCTGGACTTAGAG+AGG - chr1.4:24870801-24870820 MS.gene59174:CDS 45.0%
TAGACTCTAACCTCATCCAC+TGG + chr1.4:24868268-24868287 None:intergenic 45.0%
TAGAGACACAAAACAGACGG+CGG + chr1.4:24868814-24868833 None:intergenic 45.0%
TAGGTAACACGTCTCAAGTG+TGG - chr1.4:24870130-24870149 MS.gene59174:intron 45.0%
TCCACCATAAGTTTCCTAGC+AGG + chr1.4:24867791-24867810 None:intergenic 45.0%
TCCTGCTAGGAAACTTATGG+TGG - chr1.4:24867787-24867806 MS.gene59174:CDS 45.0%
TGAGTGCATGTTACTCTTGG+TGG + chr1.4:24870307-24870326 None:intergenic 45.0%
TGCTAAAGGAGAGGAAGGTT+TGG - chr1.4:24870921-24870940 MS.gene59174:CDS 45.0%
TGGTAGGTCCTATCACTAAG+TGG + chr1.4:24869632-24869651 None:intergenic 45.0%
TGTAGGTGACGTGGAAATAG+AGG + chr1.4:24870436-24870455 None:intergenic 45.0%
! GATTGTTGTTGTAGGTGACG+TGG + chr1.4:24870445-24870464 None:intergenic 45.0%
! GTCCAATTCATGAGTGCTGT+AGG - chr1.4:24870208-24870227 MS.gene59174:intron 45.0%
! TAGTAACCTTTTGGCAACGG+GGG + chr1.4:24870583-24870602 None:intergenic 45.0%
!! ATGGCATCGATACGCAGATA+CGG + chr1.4:24869055-24869074 None:intergenic 45.0%
!!! GAGAGGTTGATTTTGGTTGG+GGG - chr1.4:24870818-24870837 MS.gene59174:CDS 45.0%
!!! GTCCACCGCTCTTTTTTCTT+CGG - chr1.4:24870385-24870404 MS.gene59174:intron 45.0%
!! AATTTATCAAAATAAAATTA+TGG - chr1.4:24869521-24869540 MS.gene59174:intron 5.0%
!! ATTTATCAAAATAAAATTAT+GGG - chr1.4:24869522-24869541 MS.gene59174:intron 5.0%
!! TTTGATAAATTAATTAAAAT+TGG + chr1.4:24869512-24869531 None:intergenic 5.0%
!!! AACTATTTTATTTTATTTTT+TGG + chr1.4:24868365-24868384 None:intergenic 5.0%
AAGGTTCAGTCCAGTGGATG+AGG - chr1.4:24868255-24868274 MS.gene59174:CDS 50.0%
ATCAGGTAGCCCGCACATAT+CGG + chr1.4:24868617-24868636 None:intergenic 50.0%
CGGTGGAAAACCGATATGTG+CGG - chr1.4:24868604-24868623 MS.gene59174:intron 50.0%
GACCGAAGAAAAAAGAGCGG+TGG + chr1.4:24870390-24870409 None:intergenic 50.0%
GATTAAGGGAAGGTCCTGCT+AGG - chr1.4:24867774-24867793 MS.gene59174:CDS 50.0%
GCGGTGGACCATTTCATCTA+AGG + chr1.4:24870374-24870393 None:intergenic 50.0%
GGGTAGACCACATTTCACAG+TGG - chr1.4:24870753-24870772 MS.gene59174:CDS 50.0%
GGTGGAAAACCGATATGTGC+GGG - chr1.4:24868605-24868624 MS.gene59174:intron 50.0%
GGTTGGGGGAAAGCTGTATA+TGG - chr1.4:24870832-24870851 MS.gene59174:CDS 50.0%
GTAACACGTCTCAAGTGTGG+TGG - chr1.4:24870133-24870152 MS.gene59174:intron 50.0%
GTATATGGAGGACCAGCTAC+AGG - chr1.4:24870847-24870866 MS.gene59174:CDS 50.0%
TGCCATACCGGTGAGATTGA+AGG + chr1.4:24870259-24870278 None:intergenic 50.0%
TGGGGGAAAGCTGTATATGG+AGG - chr1.4:24870835-24870854 MS.gene59174:CDS 50.0%
TTCAATCCCCCGTTGCCAAA+AGG - chr1.4:24870574-24870593 MS.gene59174:CDS 50.0%
! TGAGCTTGGCTCAGTTGGTA+TGG + chr1.4:24868115-24868134 None:intergenic 50.0%
!! ATGGTGGATTGCACTGGAGA+AGG - chr1.4:24867803-24867822 MS.gene59174:CDS 50.0%
!! TGGCATCGATACGCAGATAC+GGG + chr1.4:24869054-24869073 None:intergenic 50.0%
ACACCAACTCCTCCTGTAGC+TGG + chr1.4:24870862-24870881 None:intergenic 55.0%
CGAACTTCCTCGTCTGTGTC+TGG + chr1.4:24870523-24870542 None:intergenic 55.0%
CGATTGTACGGCAACGCCAA+AGG + chr1.4:24870492-24870511 None:intergenic 55.0%
GGGATACAAGGGATATGCGG+TGG - chr1.4:24868587-24868606 MS.gene59174:intron 55.0%
GGTGGGGTCCACTTAGTGAT+AGG - chr1.4:24869621-24869640 MS.gene59174:intron 55.0%
TATGGAGGACCAGCTACAGG+AGG - chr1.4:24870850-24870869 MS.gene59174:CDS 55.0%
TCTCGTGAGCTTGGCTCAGT+TGG + chr1.4:24868120-24868139 None:intergenic 55.0%
!! GGAGGAGTTGGTGTTACACC+TGG - chr1.4:24870868-24870887 MS.gene59174:CDS 55.0%
CATGCAATGAGCGACGCGAC+GGG - chr1.4:24870181-24870200 MS.gene59174:intron 60.0%
CCATGCAATGAGCGACGCGA+CGG - chr1.4:24870180-24870199 MS.gene59174:intron 60.0%
GGACCAGCTACAGGAGGAGT+TGG - chr1.4:24870856-24870875 MS.gene59174:CDS 60.0%
! AATGAGCGACGCGACGGGTT+TGG - chr1.4:24870186-24870205 MS.gene59174:intron 60.0%
CCGTCGCGTCGCTCATTGCA+TGG + chr1.4:24870183-24870202 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 24867533 24870969 24867533 ID=MS.gene59174
chr1.4 mRNA 24867533 24870969 24867533 ID=MS.gene59174.t1;Parent=MS.gene59174
chr1.4 exon 24870532 24870969 24870532 ID=MS.gene59174.t1.exon1;Parent=MS.gene59174.t1
chr1.4 CDS 24870532 24870969 24870532 ID=cds.MS.gene59174.t1;Parent=MS.gene59174.t1
chr1.4 exon 24867533 24868369 24867533 ID=MS.gene59174.t1.exon2;Parent=MS.gene59174.t1
chr1.4 CDS 24867533 24868369 24867533 ID=cds.MS.gene59174.t1;Parent=MS.gene59174.t1
Gene Sequence

>MS.gene59174

ATGGCCCAATCTCTTTTATTCACAGTGAAGAGAAGTGCCCCTGAATTTGTAAGACCCTCAAAACCAACACCTCATGAAATAAAATTACTTTCAGATATAGATGACCAAGAAGGGTTACGTTTTCAAATTCCAGTGATACAATTTTATAAATATGATCCAAATATGGAAGGGAAAGACCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTCGTGTTTTACTATCCATTTGCAGGTAGATTAAGGGAAGGTCCTGCTAGGAAACTTATGGTGGATTGCACTGGAGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATGTTAGTTTGAAAGAATTTGGTGACGCTCTTCAACCTCCTTTTCCTTGTTTGGATGAGTTTCTTTTCGATGTTCCTGGTTATTCAGATGTGATTAACACTCCAATCCTTCTTATCCAGGTATATTATTGATATTTTCATGATGTATCAAAGGTCTCTTAATTTCTCAAAAGAAATAGAGAAATTTATTTATAGCACCGATTTTTTTTTTTTGTGGTGACCGGAATTCGAACCCCGAACTTTACATATATTATGTATTGTCCATACCAACTGAGCCAAGCTCACGAGAACATAACACAGATCATTTGAAATTACAGTTTCATTTACTTAATCCCTTTCTAATAAAATATAAGCAAACTTAATTAGTTACACACTTATTAATATAACTAGTTATTTAATTAAAAAAGGTTCAGTCCAGTGGATGAGGTTAGAGTCTAGATTTATATATTGTACCGAGTTAATGTTTGAATATCGAATAGAATTGCTATAAATGGATAGCATTTAAAGGAAGCCAAAAAATAAAATAAAATAGTTATTCAAATTTATTACTGGATTCTTATCTTTGATATATTTGTTCCATTCTCTGATGAAGCCTAGATACTTCAAAATGAATGACGTATCATATCCCTGTGTATCCTATACCGATACATGCTCGATACTTCTCGATACGTATCAGGAGAGTATCCGAATATTAATTTTTTTTTAAACAATTATCCGATACTTTTCAAATACATTTGGGATACAAGGGATATGCGGTGGAAAACCGATATGTGCGGGCTACCTGATGTTTCTATTATATTTGGTATCTATTATGTTTCTATTATACTTTTCTATTATGTTTCTACCTGATGTAATTGACTAAGTAATGATGTTTTTTATTTAGGATATATCATATATGTAATTAACTAATTGTCGCTTTCTTTATTATCAATATTATTTACATTTATATTTATATTGTTGCCGCCGTCTGTTTTGTGTCTCTATGTTTGTTTGAAAACAATGCTATTGTTTTATTGTGTGAAGGGAATTTCATTGTTGTGCCTTATTGTTTTTCTATGTCTGTTTGGTCTAGGTTTTGCGCCAATTTTTTTGCTTTTGAGTTTTAGTCCATATTTTGTATTTTGAGTTTTAATATGTAGTTTAAATTTAATTTTTTATTGAAGTATCTCAGCCGTATCGTATCTTGAATTTTAAAATTTTCCCGTATCTGCGTATCGATGCCATATCATATCATTATCGTATCATATATCAGGGCTTCATAGGTTCCATTCGAAGTAATTATATTCAAAGTGTCTTGGACACTAAATATTGATATATCATTCGATCCATGTTGTAACATTGCTTCGTTATGCTGAAAGAGTTTAACCACTTTCACTAGACTCAACATCTATACTTACATTGCACTTTCATTTTATTTAACTATATAGATACAAGTGGACATTTAATTTAACAAAATTGAATTTTAAATTAAGGACATCTTTAATATACTATCATTGAATAGATGTATTTCTCAAAAAAAGAAATCATTGAATATTGAATAGATGTATTTGTCAACAAAAAAAAAATCATTGAATAGATGTAGAAAAGTCGAAATTTGTTTATAACCTAATTAATTAACTCAAATCTTTTATTCACAATCACAAGTGTTTAATTATGGTCCAATTTTAATTAATTTATCAAAATAAAATTATGGGGTCAAGATTCTCTCCATTTATAACTTTTTCCATTTCTTGCAATTTGGAGAGAGATAGACACATAAAAAATAATGATGGTGGGGTCCACTTAGTGATAGGACCTACCACTTATTTTTGTGTGTCTATCTCTCTCCAAATTGCAAGAAATGGAAAAAGTTATAAATGGAGAGGATCTTTACCCAAAATTATGATTAGGAAAATACTAAATGGTACGACAATGTTTTCGCTTTAATTGCACGACACTACTAACTTGCTTCTTTTTTAATTTAAATATAACTTAAAATAATGCATTCAATTCCTGATTTATTCTTTTACAAGAAATCACAAGTTATAAATTACTAGTACATCATATTTCTTGCCACTTCTCCGATGTTTTGTCTTGTTTGATACTAACAATCCAATACTTAATGGGGTTAAAAGAGTTTTGAGTTTTGATCTTAAAGTCTTTGATTTTCTTTTTACCGGAAACTTGCATGTTGTTCAAGGGTTCGTGCGTATTCGTGTGTCAAGTATGTCGCACCATATATCATATCTCTTATGATTACCTTCATTCACATAAATTCATAATTGAATTGTAATTGTGTGTAGGTAACACGTCTCAAGTGTGGTGGTTTCATTTTTGCTCTTCGTCTAAACCATGCAATGAGCGACGCGACGGGTTTGGTCCAATTCATGAGTGCTGTAGGTGAAATTTCTTGCGGGATGAATGAACCTTCAATCTCACCGGTATGGCATAGAGAGCTTCTAAACGCAAGGGATCCACCAAGAGTAACATGCACTCATCGTGAATACGAACAAGTTCCTGACACAAAAGGAACCATTACACCCTTAGATGAAATGGTCCACCGCTCTTTTTTCTTCGGTCCAACTGAGTTATCTGCACTTCGTCCTCTATTTCCACGTCACCTACAACAACAATCCAAATTCGAAATCATCACTGCATGCCTTTGGCGTTGCCGTACAATCGCATTACAACCAGACACAGACGAGGAAGTTCGCATAATTTGCTTATACAATGCACGTCGCAAGTTCAATCCCCCGTTGCCAAAAGGTTACTATGGAAATGCTTTTGCGTTTCCTGTTGCAATTACAACTGCAGGAAAACTAATTGAAAATCCATTAGGGTATGCACTTGAGTTAGTTAAAAAAGCTAAATCAGATGTCACTCAAGAATACATGCATTCAGTTGCGGATTTAATGGTTATCAAGGGTAGACCACATTTCACAGTGGTGAGAATGTACTTAGTGTCTGATGTTACACATGCTGGACTTAGAGAGGTTGATTTTGGTTGGGGGAAAGCTGTATATGGAGGACCAGCTACAGGAGGAGTTGGTGTTACACCTGGTGTTGCTAGCTTTTATATACCTTTCAAAAATGCTAAAGGAGAGGAAGGTTTGGTTATACCACTTTGTTTGCCAACTTAA
Protein sequence

>MS.gene59174.t1

MAQSLLFTVKRSAPEFVRPSKPTPHEIKLLSDIDDQEGLRFQIPVIQFYKYDPNMEGKDPVDIIRKALAKTLVFYYPFAGRLREGPARKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDEFLFDVPGYSDVINTPILLIQVTRLKCGGFIFALRLNHAMSDATGLVQFMSAVGEISCGMNEPSISPVWHRELLNARDPPRVTCTHREYEQVPDTKGTITPLDEMVHRSFFFGPTELSALRPLFPRHLQQQSKFEIITACLWRCRTIALQPDTDEEVRIICLYNARRKFNPPLPKGYYGNAFAFPVAITTAGKLIENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRMYLVSDVTHAGLREVDFGWGKAVYGGPATGGVGVTPGVASFYIPFKNAKGEEGLVIPLCLPT