Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61143.t1 | XP_013453854.1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 285 | 358 | 7.10E-32 | 146 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61143.t1 | D4N502 | 52.1 | 71 | 34 | 0 | 1 | 71 | 290 | 360 | 5.1e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61143.t1 | A0A072UPY1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 285 | 358 | 5.1e-32 | 146.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene61143 | 0.83092 | 2.24E-55 | -1.69E-46 |
| MS.gene050380 | MS.gene61143 | 0.806219 | 9.15E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene61143 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene61143 | 0.815054 | 1.13E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene61143 | 0.804434 | 2.16E-49 | -1.69E-46 |
| MS.gene051844 | MS.gene61143 | 0.804704 | 1.90E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene61143 | 0.835694 | 1.45E-56 | -1.69E-46 |
| MS.gene05240 | MS.gene61143 | 0.837223 | 5.90E-57 | -1.69E-46 |
| MS.gene052606 | MS.gene61143 | 0.827096 | 1.89E-54 | -1.69E-46 |
| MS.gene053031 | MS.gene61143 | 0.825922 | 3.60E-54 | -1.69E-46 |
| MS.gene053033 | MS.gene61143 | 0.808883 | 2.49E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene61143 | 0.818898 | 1.54E-52 | -1.69E-46 |
| MS.gene053406 | MS.gene61143 | 0.867141 | 1.70E-65 | -1.69E-46 |
| MS.gene053420 | MS.gene61143 | 0.821489 | 3.93E-53 | -1.69E-46 |
| MS.gene054146 | MS.gene61143 | 0.822411 | 2.40E-53 | -1.69E-46 |
| MS.gene054181 | MS.gene61143 | 0.835872 | 1.30E-56 | -1.69E-46 |
| MS.gene054742 | MS.gene61143 | 0.801402 | 9.16E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene61143 | 0.80515 | 1.53E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene61143 | 0.820269 | 7.50E-53 | -1.69E-46 |
| MS.gene055159 | MS.gene61143 | 0.807381 | 5.20E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene61143 | 0.827377 | 1.62E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene61143 | -0.809251 | 2.08E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene61143 | 0.801284 | 9.68E-49 | -1.69E-46 |
| MS.gene056218 | MS.gene61143 | 0.835706 | 1.44E-56 | -1.69E-46 |
| MS.gene056298 | MS.gene61143 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene61143 | 0.806145 | 9.48E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene61143 | 0.806836 | 6.78E-50 | -1.69E-46 |
| MS.gene056680 | MS.gene61143 | 0.801529 | 8.63E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene61143 | 0.80487 | 1.76E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene61143 | 0.808101 | 3.65E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene61143 | 0.813991 | 1.93E-51 | -1.69E-46 |
| MS.gene057768 | MS.gene61143 | 0.814192 | 1.75E-51 | -1.69E-46 |
| MS.gene058109 | MS.gene61143 | 0.814393 | 1.58E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene61143 | 0.83399 | 3.88E-56 | -1.69E-46 |
| MS.gene058173 | MS.gene61143 | 0.821912 | 3.14E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene61143 | 0.834476 | 2.93E-56 | -1.69E-46 |
| MS.gene058177 | MS.gene61143 | 0.803938 | 2.74E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene61143 | -0.807115 | 5.92E-50 | -1.69E-46 |
| MS.gene059037 | MS.gene61143 | 0.804196 | 2.43E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene61143 | 0.809468 | 1.86E-50 | -1.69E-46 |
| MS.gene059402 | MS.gene61143 | 0.806967 | 6.36E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene61143 | 0.810708 | 1.01E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene61143 | 0.821489 | 3.93E-53 | -1.69E-46 |
| MS.gene060087 | MS.gene61143 | 0.813877 | 2.05E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene61143 | 0.825499 | 4.54E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGTAATAAGGCTTGGTAC+TGG | 0.450966 | 5.1:+37378517 | None:intergenic |
| TATTTCTCACGCCAGCTTGA+AGG | 0.471903 | 5.1:-37378442 | MS.gene61143:CDS |
| CTAATGTCGTTGAACACTGC+AGG | 0.481272 | 5.1:+37378487 | None:intergenic |
| AGTGTGGAAGATTTCTTCAA+AGG | 0.508864 | 5.1:-37378466 | MS.gene61143:CDS |
| TAATGTCGTTGAACACTGCA+GGG | 0.582551 | 5.1:+37378488 | None:intergenic |
| CAATCTAGCACTGTGAAATG+TGG | 0.587201 | 5.1:+37378555 | None:intergenic |
| CGATTAACTCAGAGAAGGAG+AGG | 0.590103 | 5.1:-37378588 | MS.gene61143:CDS |
| AGCACTGTGAAATGTGGCAA+TGG | 0.625118 | 5.1:+37378561 | None:intergenic |
| AGCAACGATTAACTCAGAGA+AGG | 0.647001 | 5.1:-37378593 | MS.gene61143:CDS |
| AAATGTACATTGATGACATG+AGG | 0.655321 | 5.1:-37378417 | MS.gene61143:CDS |
| AATGTCGTTGAACACTGCAG+GGG | 0.691919 | 5.1:+37378489 | None:intergenic |
| AGTGTTCAACGACATTAGTG+TGG | 0.716232 | 5.1:-37378482 | MS.gene61143:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGTACATTGATGACATG+AGG | - | chr5.1:37378590-37378609 | MS.gene61143:CDS | 30.0% |
| AGTGTGGAAGATTTCTTCAA+AGG | - | chr5.1:37378541-37378560 | MS.gene61143:CDS | 35.0% | |
| ! | TGTACATTTTTCCTTCAAGC+TGG | + | chr5.1:37378579-37378598 | None:intergenic | 35.0% |
| !!! | GTTTTAGGAGTAATAAGGCT+TGG | + | chr5.1:37378499-37378518 | None:intergenic | 35.0% |
| AGCAACGATTAACTCAGAGA+AGG | - | chr5.1:37378414-37378433 | MS.gene61143:CDS | 40.0% | |
| AGTGTTCAACGACATTAGTG+TGG | - | chr5.1:37378525-37378544 | MS.gene61143:CDS | 40.0% | |
| CAATCTAGCACTGTGAAATG+TGG | + | chr5.1:37378455-37378474 | None:intergenic | 40.0% | |
| TAATGTCGTTGAACACTGCA+GGG | + | chr5.1:37378522-37378541 | None:intergenic | 40.0% | |
| !!! | CAGGGGTTTTAGGAGTAATA+AGG | + | chr5.1:37378504-37378523 | None:intergenic | 40.0% |
| AATGTCGTTGAACACTGCAG+GGG | + | chr5.1:37378521-37378540 | None:intergenic | 45.0% | |
| AGCACTGTGAAATGTGGCAA+TGG | + | chr5.1:37378449-37378468 | None:intergenic | 45.0% | |
| CGATTAACTCAGAGAAGGAG+AGG | - | chr5.1:37378419-37378438 | MS.gene61143:CDS | 45.0% | |
| CTAATGTCGTTGAACACTGC+AGG | + | chr5.1:37378523-37378542 | None:intergenic | 45.0% | |
| TATTTCTCACGCCAGCTTGA+AGG | - | chr5.1:37378565-37378584 | MS.gene61143:CDS | 45.0% | |
| ! | GGAGTAATAAGGCTTGGTAC+TGG | + | chr5.1:37378493-37378512 | None:intergenic | 45.0% |
| ! | TTGAACACTGCAGGGGTTTT+AGG | + | chr5.1:37378514-37378533 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 37378402 | 37378627 | 37378402 | ID=MS.gene61143 |
| chr5.1 | mRNA | 37378402 | 37378627 | 37378402 | ID=MS.gene61143.t1;Parent=MS.gene61143 |
| chr5.1 | exon | 37378402 | 37378627 | 37378402 | ID=MS.gene61143.t1.exon1;Parent=MS.gene61143.t1 |
| chr5.1 | CDS | 37378402 | 37378627 | 37378402 | ID=cds.MS.gene61143.t1;Parent=MS.gene61143.t1 |
| Gene Sequence |
| Protein sequence |
