AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64934


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64934.t1 MTR_2g069350 42.407 349 135 10 1 298 32 365 2.62e-75 235
MS.gene64934.t1 MTR_4g083540 36.000 300 135 8 9 298 40 292 7.67e-55 180
MS.gene64934.t1 MTR_2g028830 35.354 297 150 5 5 298 61 318 2.15e-54 179
MS.gene64934.t1 MTR_5g013690 32.000 225 128 3 74 298 135 334 2.13e-31 120
MS.gene64934.t1 MTR_5g013690 32.000 225 128 3 74 298 72 271 2.93e-31 118
MS.gene64934.t1 MTR_1g019250 28.251 223 134 5 74 296 142 338 1.62e-23 98.6
MS.gene64934.t1 MTR_4g099060 30.667 225 128 3 74 298 89 285 2.70e-23 97.1
MS.gene64934.t1 MTR_4g099060 30.667 225 128 3 74 298 134 330 2.75e-23 97.8
MS.gene64934.t1 MTR_4g036485 26.457 223 118 5 66 286 21 199 1.41e-20 88.2
MS.gene64934.t1 MTR_3g117970 27.706 231 112 6 69 294 11 191 1.72e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64934.t1 AT3G12920 40.550 291 128 9 32 298 64 333 1.82e-61 198
MS.gene64934.t1 AT1G79110 43.668 229 116 4 72 298 114 331 2.12e-56 185
MS.gene64934.t1 AT1G79110 43.668 229 116 4 72 298 136 353 2.54e-56 186
MS.gene64934.t1 AT5G45100 38.403 263 120 7 38 298 43 265 1.70e-55 181
MS.gene64934.t1 AT5G45100 38.403 263 120 7 38 298 70 292 5.09e-55 180
MS.gene64934.t1 AT1G79110 43.103 232 116 5 72 298 136 356 3.67e-54 180
MS.gene64934.t1 AT4G19700 37.860 243 117 5 59 298 91 302 1.73e-48 164
MS.gene64934.t1 AT4G35070 31.963 219 118 6 79 296 19 207 8.32e-26 102
MS.gene64934.t1 AT4G35070 32.258 217 116 6 79 294 74 260 1.51e-25 102
MS.gene64934.t1 AT1G45976 28.251 223 127 5 74 296 134 323 3.73e-23 97.4
MS.gene64934.t1 AT1G32740 28.700 223 129 6 75 294 68 263 6.40e-23 95.9
MS.gene64934.t1 AT1G32740 28.700 223 129 6 75 294 68 263 6.40e-23 95.9
MS.gene64934.t1 AT1G32740 28.700 223 129 6 75 294 101 296 7.99e-23 96.3
MS.gene64934.t1 AT1G32740 28.700 223 129 6 75 294 112 307 8.21e-23 96.3
MS.gene64934.t1 AT1G60610 27.706 231 146 5 66 296 128 337 1.38e-22 96.3
MS.gene64934.t1 AT1G60610 27.706 231 146 5 66 296 128 337 1.38e-22 96.3
MS.gene64934.t1 AT1G60610 27.706 231 146 5 66 296 128 337 1.38e-22 96.3
MS.gene64934.t1 AT1G60610 27.706 231 146 5 66 296 128 337 1.38e-22 96.3
MS.gene64934.t1 AT1G60610 27.706 231 146 5 66 296 128 337 1.38e-22 96.3
MS.gene64934.t1 AT4G17680 30.909 220 123 7 78 296 121 312 6.10e-21 91.3
MS.gene64934.t1 AT4G17680 30.909 220 123 7 78 296 49 240 8.72e-21 89.4
MS.gene64934.t1 AT1G10650 28.837 215 128 4 83 296 90 280 1.42e-20 89.7
MS.gene64934.t1 AT1G10650 27.570 214 132 3 83 296 146 336 2.02e-20 90.1
MS.gene64934.t1 AT5G47050 27.530 247 149 4 54 296 77 297 1.12e-17 82.0
MS.gene64934.t1 AT4G17680 31.731 208 112 8 90 296 10 188 5.06e-17 77.8

Find 45 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCACTGTTACCACTTTC+AGG 0.214320 8.2:-50123881 MS.gene64934:CDS
AGGGTAGTTATTGTTATTGA+TGG 0.337872 8.2:+50123700 None:intergenic
GAGAGAAACAATGATGATAA+TGG 0.367299 8.2:-50123471 MS.gene64934:CDS
TGCCGTTGCTGCGGCGGTGG+AGG 0.390131 8.2:-50123192 MS.gene64934:CDS
GTGTTCAATGTGTGGGGCTA+TGG 0.402214 8.2:-50122985 MS.gene64934:CDS
GATTTAACAGAATCACAAAT+AGG 0.421677 8.2:+50122951 None:intergenic
ATATTCATCATTACTACAAC+AGG 0.424115 8.2:+50123154 None:intergenic
CGTTGCTGCGGCGGTGGAGG+CGG 0.430174 8.2:-50123189 MS.gene64934:CDS
TGCTGCGGCGGTGGAGGCGG+AGG 0.433823 8.2:-50123186 MS.gene64934:CDS
AGTAATGACGGCGCAGGATA+AGG 0.449129 8.2:-50123114 MS.gene64934:CDS
TATGCATGGAGAATCAAATC+TGG 0.461576 8.2:-50123346 MS.gene64934:CDS
TATGTTTGTGTTCAATGTGT+GGG 0.464104 8.2:-50122992 MS.gene64934:CDS
GAAGATGGTGTCTGGTTTCA+CGG 0.465973 8.2:+50123769 None:intergenic
CGCCTCCACCGCCGCAGCAA+CGG 0.480109 8.2:+50123190 None:intergenic
TTGTAAGCGAAGATGGTGTC+TGG 0.480918 8.2:+50123761 None:intergenic
ATGAATATTGTGGGTGGCGT+AGG 0.488245 8.2:-50123139 MS.gene64934:CDS
AACGTTGTTGTAAGCGAAGA+TGG 0.493808 8.2:+50123754 None:intergenic
CACAAACATAAGTGTCTACA+AGG 0.505076 8.2:+50123005 None:intergenic
CGGAGGAAATCGGTTATGAA+AGG 0.505897 8.2:+50123806 None:intergenic
AAGGATGAAGGTACTAGTAG+TGG 0.520250 8.2:-50123095 MS.gene64934:CDS
AAAAGTGAAAACACTATGCA+TGG 0.524997 8.2:-50123360 MS.gene64934:CDS
AACAACAACAACGGCTGCGA+CGG 0.528600 8.2:-50123236 MS.gene64934:CDS
TGTAGTAATGATGAATATTG+TGG 0.529768 8.2:-50123149 MS.gene64934:CDS
ATAGATTATGCAGAAACTGT+GGG 0.542740 8.2:-50123055 MS.gene64934:CDS
TTATGTTTGTGTTCAATGTG+TGG 0.554213 8.2:-50122993 MS.gene64934:CDS
TTAGACGGAGAAGTTTAGAG+TGG 0.557294 8.2:-50123513 MS.gene64934:intron
AATAGATTATGCAGAAACTG+TGG 0.573162 8.2:-50123056 MS.gene64934:CDS
GTAGTAATGATGAATATTGT+GGG 0.574573 8.2:-50123148 MS.gene64934:CDS
GGAGGAAATCGGTTATGAAA+GGG 0.577225 8.2:+50123807 None:intergenic
TTATGCAGAAACTGTGGGGA+AGG 0.589265 8.2:-50123050 MS.gene64934:CDS
ATGTTTGTGTTCAATGTGTG+GGG 0.595264 8.2:-50122991 MS.gene64934:CDS
ACGGCGCAGGATAAGGATGA+AGG 0.596413 8.2:-50123107 MS.gene64934:CDS
TGAATATTGTGGGTGGCGTA+GGG 0.602718 8.2:-50123138 MS.gene64934:CDS
GTTATGAAAGGGTTGTTCGA+CGG 0.613447 8.2:+50123818 None:intergenic
ACGGTGGTTGTGCCTGAAAG+TGG 0.615359 8.2:+50123869 None:intergenic
TTGTTCGACGGAGATTGCGA+CGG 0.619398 8.2:+50123830 None:intergenic
TAGGGTAGTAATGACGGCGC+AGG 0.626421 8.2:-50123120 MS.gene64934:CDS
GTGGCGTAGGGTAGTAATGA+CGG 0.629194 8.2:-50123126 MS.gene64934:CDS
TTCAGGCACAACCACCGTGT+CGG 0.634510 8.2:-50123864 MS.gene64934:CDS
AGAAATGAAGTTGTCGATGT+CGG 0.644465 8.2:+50123634 None:intergenic
TGCGACGGTGAATATAGCGG+TGG 0.671652 8.2:-50123221 MS.gene64934:CDS
GGCTGCGACGGTGAATATAG+CGG 0.693061 8.2:-50123224 MS.gene64934:CDS
TAGATTATGCAGAAACTGTG+GGG 0.698504 8.2:-50123054 MS.gene64934:CDS
GTAATGATGAATATTGTGGG+TGG 0.732674 8.2:-50123145 MS.gene64934:CDS
GATGGTGTCTGGTTTCACGG+TGG 0.760177 8.2:+50123772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAATTTAGGTGAATA+AGG + chr8.2:50123229-50123248 None:intergenic 20.0%
!!! TGTTCATTCTTTCAATTTTT+TGG + chr8.2:50123426-50123445 None:intergenic 20.0%
! AAAAATCTTCACTGATGAAA+GGG + chr8.2:50123141-50123160 None:intergenic 25.0%
! AAATTTAGGTGAATAAGGTA+AGG + chr8.2:50123224-50123243 None:intergenic 25.0%
! ATATTCATCATTACTACAAC+AGG + chr8.2:50123668-50123687 None:intergenic 25.0%
! GATTTAACAGAATCACAAAT+AGG + chr8.2:50123871-50123890 None:intergenic 25.0%
! GTAGTAATGATGAATATTGT+GGG - chr8.2:50123671-50123690 MS.gene64934:CDS 25.0%
! TGTAGTAATGATGAATATTG+TGG - chr8.2:50123670-50123689 MS.gene64934:CDS 25.0%
AAAAGTGAAAACACTATGCA+TGG - chr8.2:50123459-50123478 MS.gene64934:CDS 30.0%
AATAGATTATGCAGAAACTG+TGG - chr8.2:50123763-50123782 MS.gene64934:CDS 30.0%
ATAGATTATGCAGAAACTGT+GGG - chr8.2:50123764-50123783 MS.gene64934:CDS 30.0%
CAAAACAACAACAACAACAA+CGG - chr8.2:50123574-50123593 MS.gene64934:intron 30.0%
GAAAAATCTTCACTGATGAA+AGG + chr8.2:50123142-50123161 None:intergenic 30.0%
GAGAGAAACAATGATGATAA+TGG - chr8.2:50123348-50123367 MS.gene64934:CDS 30.0%
TATGTTTGTGTTCAATGTGT+GGG - chr8.2:50123827-50123846 MS.gene64934:CDS 30.0%
TTATGTTTGTGTTCAATGTG+TGG - chr8.2:50123826-50123845 MS.gene64934:CDS 30.0%
! AGGGTAGTTATTGTTATTGA+TGG + chr8.2:50123122-50123141 None:intergenic 30.0%
!! TGTTTTGCATTTGTTGCATA+AGG + chr8.2:50123561-50123580 None:intergenic 30.0%
!!! AATCTCTAGAACGTTTTTGT+GGG + chr8.2:50123099-50123118 None:intergenic 30.0%
!!! TATTTTTTGACGGAGGAAAT+CGG + chr8.2:50123026-50123045 None:intergenic 30.0%
!!! TGCATGTGTGTTTTTTTAGA+CGG - chr8.2:50123291-50123310 MS.gene64934:CDS 30.0%
AGAAATGAAGTTGTCGATGT+CGG + chr8.2:50123188-50123207 None:intergenic 35.0%
ATGTTTGTGTTCAATGTGTG+GGG - chr8.2:50123828-50123847 MS.gene64934:CDS 35.0%
CACAAACATAAGTGTCTACA+AGG + chr8.2:50123817-50123836 None:intergenic 35.0%
GTAATGATGAATATTGTGGG+TGG - chr8.2:50123674-50123693 MS.gene64934:CDS 35.0%
TAGATTATGCAGAAACTGTG+GGG - chr8.2:50123765-50123784 MS.gene64934:CDS 35.0%
TATGCATGGAGAATCAAATC+TGG - chr8.2:50123473-50123492 MS.gene64934:CDS 35.0%
!!! GAATCTCTAGAACGTTTTTG+TGG + chr8.2:50123100-50123119 None:intergenic 35.0%
!!! TCACGGTGGATATTTTTTGA+CGG + chr8.2:50123036-50123055 None:intergenic 35.0%
AACGTTGTTGTAAGCGAAGA+TGG + chr8.2:50123068-50123087 None:intergenic 40.0%
GGAGGAAATCGGTTATGAAA+GGG + chr8.2:50123015-50123034 None:intergenic 40.0%
GTTATGAAAGGGTTGTTCGA+CGG + chr8.2:50123004-50123023 None:intergenic 40.0%
TCATCACTGTTACCACTTTC+AGG - chr8.2:50122938-50122957 MS.gene64934:CDS 40.0%
TTAGACGGAGAAGTTTAGAG+TGG - chr8.2:50123306-50123325 MS.gene64934:CDS 40.0%
!! AAGGATGAAGGTACTAGTAG+TGG - chr8.2:50123724-50123743 MS.gene64934:CDS 40.0%
ATGAATATTGTGGGTGGCGT+AGG - chr8.2:50123680-50123699 MS.gene64934:CDS 45.0%
CGGAGGAAATCGGTTATGAA+AGG + chr8.2:50123016-50123035 None:intergenic 45.0%
TGAATATTGTGGGTGGCGTA+GGG - chr8.2:50123681-50123700 MS.gene64934:CDS 45.0%
TTATGCAGAAACTGTGGGGA+AGG - chr8.2:50123769-50123788 MS.gene64934:CDS 45.0%
TTGTAAGCGAAGATGGTGTC+TGG + chr8.2:50123061-50123080 None:intergenic 45.0%
! AATGAGATTTTCCGACACGG+TGG + chr8.2:50122969-50122988 None:intergenic 45.0%
! CGGAATGAGATTTTCCGACA+CGG + chr8.2:50122972-50122991 None:intergenic 45.0%
! GAAGATGGTGTCTGGTTTCA+CGG + chr8.2:50123053-50123072 None:intergenic 45.0%
!! TGTCGATGTCGGTGTTTTGA+TGG + chr8.2:50123177-50123196 None:intergenic 45.0%
!!! CGGTGGATATTTTTTGACGG+AGG + chr8.2:50123033-50123052 None:intergenic 45.0%
!! AAATAAAAAAACAAAAATTT+AGG + chr8.2:50123238-50123257 None:intergenic 5.0%
AACAACAACAACGGCTGCGA+CGG - chr8.2:50123583-50123602 MS.gene64934:intron 50.0%
AGTAATGACGGCGCAGGATA+AGG - chr8.2:50123705-50123724 MS.gene64934:CDS 50.0%
GTGGCGTAGGGTAGTAATGA+CGG - chr8.2:50123693-50123712 MS.gene64934:CDS 50.0%
GTGTTCAATGTGTGGGGCTA+TGG - chr8.2:50123834-50123853 MS.gene64934:CDS 50.0%
TTGTTCGACGGAGATTGCGA+CGG + chr8.2:50122992-50123011 None:intergenic 50.0%
! TGGTGATTTTGCCGTTGCTG+CGG - chr8.2:50123618-50123637 MS.gene64934:intron 50.0%
!!! CGATGTCGGTGTTTTGATGG+TGG + chr8.2:50123174-50123193 None:intergenic 50.0%
ACGGCGCAGGATAAGGATGA+AGG - chr8.2:50123712-50123731 MS.gene64934:CDS 55.0%
ACGGTGGTTGTGCCTGAAAG+TGG + chr8.2:50122953-50122972 None:intergenic 55.0%
GGCTGCGACGGTGAATATAG+CGG - chr8.2:50123595-50123614 MS.gene64934:intron 55.0%
TAGGGTAGTAATGACGGCGC+AGG - chr8.2:50123699-50123718 MS.gene64934:CDS 55.0%
TGCGACGGTGAATATAGCGG+TGG - chr8.2:50123598-50123617 MS.gene64934:intron 55.0%
TTCAGGCACAACCACCGTGT+CGG - chr8.2:50122955-50122974 MS.gene64934:CDS 55.0%
! GATGGTGTCTGGTTTCACGG+TGG + chr8.2:50123050-50123069 None:intergenic 55.0%
! TGATTTTGCCGTTGCTGCGG+CGG - chr8.2:50123621-50123640 MS.gene64934:intron 55.0%
! TTTTGCCGTTGCTGCGGCGG+TGG - chr8.2:50123624-50123643 MS.gene64934:intron 65.0%
CGCCTCCACCGCCGCAGCAA+CGG + chr8.2:50123632-50123651 None:intergenic 75.0%
CGTTGCTGCGGCGGTGGAGG+CGG - chr8.2:50123630-50123649 MS.gene64934:CDS 75.0%
TGCCGTTGCTGCGGCGGTGG+AGG - chr8.2:50123627-50123646 MS.gene64934:intron 75.0%
! TGCTGCGGCGGTGGAGGCGG+AGG - chr8.2:50123633-50123652 MS.gene64934:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 50122923 50123918 50122923 ID=MS.gene64934
chr8.2 mRNA 50122923 50123918 50122923 ID=MS.gene64934.t1;Parent=MS.gene64934
chr8.2 exon 50123628 50123918 50123628 ID=MS.gene64934.t1.exon1;Parent=MS.gene64934.t1
chr8.2 CDS 50123628 50123918 50123628 ID=cds.MS.gene64934.t1;Parent=MS.gene64934.t1
chr8.2 exon 50122923 50123531 50122923 ID=MS.gene64934.t1.exon2;Parent=MS.gene64934.t1
chr8.2 CDS 50122923 50123531 50122923 ID=cds.MS.gene64934.t1;Parent=MS.gene64934.t1
Gene Sequence

>MS.gene64934

ATGTACAACAACTACTCATCACTGTTACCACTTTCAGGCACAACCACCGTGTCGGAAAATCTCATTCCGTCGCAATCTCCGTCGAACAACCCTTTCATAACCGATTTCCTCCGTCAAAAAATATCCACCGTGAAACCAGACACCATCTTCGCTTACAACAACGTTGTCTCGTTCCCACAAAAACGTTCTAGAGATTCCATCAATAACAATAACTACCCTTTCATCAGTGAAGATTTTTCTCATTACATCCACCATCAAAACACCGACATCGACAACTTCATTTCTCAACATGTAAATTCCTTACCTTATTCACCTAAATTTTTGTTTTTTTATTTATTTATTAAAAAAAAAAACTGATTTGTTTTTTTTGCATGTGTGTTTTTTTAGACGGAGAAGTTTAGAGTGGAATTAGAAGAGAAAAGAAAGAGAGAAACAATGATGATAATGGAAGCAATAGAAAACGAAGTGATGAAGAAAATGAAAGCAAAAGAAGAAGAAATCCAAAAAATTGAAAGAATGAACATGATTCTTGAAGAAAAAGTGAAAACACTATGCATGGAGAATCAAATCTGGAGAGAGTTAGCACAAAACAATGAAGCAACAGCAAATGCTCTCAGAAACAATCTACAACAAATCCTTATGCAACAAATGCAAAACAACAACAACAACAACGGCTGCGACGGTGAATATAGCGGTGGTGATTTTGCCGTTGCTGCGGCGGTGGAGGCGGAGGATGCTGAGTCCTGTTGTAGTAATGATGAATATTGTGGGTGGCGTAGGGTAGTAATGACGGCGCAGGATAAGGATGAAGGTACTAGTAGTGGTAGAGTGATGAATAATAATAGATTATGCAGAAACTGTGGGGAAGGTGAATCTTGTGTTTTGATCATGCCTTGTAGACACTTATGTTTGTGTTCAATGTGTGGGGCTATGGTTCTTACTTGTCCTATTTGTGATTCTGTTAAATCTGCTACTCTTAATGTTAATCTTGATTAA

Protein sequence

>MS.gene64934.t1

MYNNYSSLLPLSGTTTVSENLIPSQSPSNNPFITDFLRQKISTVKPDTIFAYNNVVSFPQKRSRDSINNNNYPFISEDFSHYIHHQNTDIDNFISQHTEKFRVELEEKRKRETMMIMEAIENEVMKKMKAKEEEIQKIERMNMILEEKVKTLCMENQIWRELAQNNEATANALRNNLQQILMQQMQNNNNNNGCDGEYSGGDFAVAAAVEAEDAESCCSNDEYCGWRRVVMTAQDKDEGTSSGRVMNNNRLCRNCGEGESCVLIMPCRHLCLCSMCGAMVLTCPICDSVKSATLNVNLD