AlfalfaGEDB | MS.gene66476

M. sativa cultivar XinJiangDaYe / MS.gene66476

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66476.t1	XP_003589041.1	97.7	434	10	0	1	434	1	434	3.50E-224	787.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66476.t1	Q058P4	59.0	407	161	4	28	434	48	448	1.4e-128	461.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66476.t1	G7IBA8	97.7	434	10	0	1	434	1	434	2.6e-224	787.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene66476	0.820023	8.54E-53	-1.69E-46
MS.gene049208	MS.gene66476	0.819077	1.40E-52	-1.69E-46
MS.gene049368	MS.gene66476	0.824142	9.47E-54	-1.69E-46
MS.gene04998	MS.gene66476	0.801825	7.50E-49	-1.69E-46
MS.gene050253	MS.gene66476	0.863704	2.06E-64	-1.69E-46
MS.gene050838	MS.gene66476	0.801268	9.76E-49	-1.69E-46
MS.gene051343	MS.gene66476	0.831789	1.37E-55	-1.69E-46
MS.gene051676	MS.gene66476	0.973693	7.30E-137	-1.69E-46
MS.gene05188	MS.gene66476	0.804784	1.83E-49	-1.69E-46
MS.gene051902	MS.gene66476	0.836962	6.88E-57	-1.69E-46
MS.gene051975	MS.gene66476	0.871226	8.03E-67	-1.69E-46
MS.gene052072	MS.gene66476	0.81882	1.61E-52	-1.69E-46
MS.gene052434	MS.gene66476	0.82716	1.82E-54	-1.69E-46
MS.gene052609	MS.gene66476	0.807475	4.96E-50	-1.69E-46
MS.gene052932	MS.gene66476	0.800577	1.35E-48	-1.69E-46
MS.gene053045	MS.gene66476	0.80004	1.74E-48	-1.69E-46
MS.gene053298	MS.gene66476	0.831607	1.52E-55	-1.69E-46
MS.gene053340	MS.gene66476	0.821602	3.70E-53	-1.69E-46
MS.gene053564	MS.gene66476	0.808946	2.41E-50	-1.69E-46
MS.gene05364	MS.gene66476	0.814607	1.41E-51	-1.69E-46
MS.gene05458	MS.gene66476	0.817461	3.26E-52	-1.69E-46
MS.gene05469	MS.gene66476	0.852115	5.70E-61	-1.69E-46
MS.gene054892	MS.gene66476	0.802773	4.79E-49	-1.69E-46
MS.gene054893	MS.gene66476	0.805339	1.40E-49	-1.69E-46
MS.gene055311	MS.gene66476	0.83897	2.09E-57	-1.69E-46
MS.gene055313	MS.gene66476	0.844848	5.86E-59	-1.69E-46
MS.gene055314	MS.gene66476	0.838598	2.62E-57	-1.69E-46
MS.gene055513	MS.gene66476	0.84111	5.80E-58	-1.69E-46
MS.gene055603	MS.gene66476	0.819966	8.80E-53	-1.69E-46
MS.gene056045	MS.gene66476	0.801989	6.94E-49	-1.69E-46
MS.gene056181	MS.gene66476	0.818705	1.70E-52	-1.69E-46
MS.gene056986	MS.gene66476	0.855226	7.27E-62	-1.69E-46
MS.gene057102	MS.gene66476	0.815649	8.30E-52	-1.69E-46
MS.gene057999	MS.gene66476	0.81627	6.03E-52	-1.69E-46
MS.gene058639	MS.gene66476	0.801129	1.04E-48	-1.69E-46
MS.gene059146	MS.gene66476	0.801848	7.42E-49	-1.69E-46
MS.gene059171	MS.gene66476	0.820268	7.51E-53	-1.69E-46
MS.gene059227	MS.gene66476	0.815589	8.55E-52	-1.69E-46
MS.gene05985	MS.gene66476	-0.815861	7.44E-52	-1.69E-46
MS.gene06011	MS.gene66476	0.823072	1.69E-53	-1.69E-46
MS.gene060259	MS.gene66476	0.815758	7.84E-52	-1.69E-46
MS.gene060365	MS.gene66476	0.82534	4.95E-54	-1.69E-46
MS.gene060933	MS.gene66476	0.83036	3.07E-55	-1.69E-46
MS.gene060935	MS.gene66476	0.811045	8.52E-51	-1.69E-46
MS.gene060937	MS.gene66476	0.829458	5.09E-55	-1.69E-46
MS.gene061100	MS.gene66476	0.800232	1.59E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66476.t1	MTR_1g017770	97.696	434	10	0	1	434	1	434	0.0	859
MS.gene66476.t1	MTR_5g015210	63.882	407	141	4	28	434	51	451	0.0	527
MS.gene66476.t1	MTR_8g092870	29.167	408	237	13	45	417	10	400	3.05e-35	135
MS.gene66476.t1	MTR_4g085720	26.382	398	259	7	36	413	5	388	6.42e-32	125
MS.gene66476.t1	MTR_5g077510	27.792	403	237	10	45	413	35	417	4.09e-30	121
MS.gene66476.t1	MTR_7g106340	28.095	420	260	11	44	431	7	416	1.03e-27	114
MS.gene66476.t1	MTR_4g485520	28.198	344	221	8	45	372	105	438	1.26e-27	115
MS.gene66476.t1	MTR_4g063800	26.425	386	256	9	45	415	255	627	1.85e-27	115
MS.gene66476.t1	MTR_4g485520	28.198	344	221	8	45	372	273	606	1.95e-27	115
MS.gene66476.t1	MTR_3g095730	27.295	403	249	13	45	417	13	401	5.63e-27	112
MS.gene66476.t1	MTR_6g071340	26.434	401	253	10	45	413	5	395	1.11e-26	111
MS.gene66476.t1	MTR_3g065080	25.917	409	260	10	31	413	26	417	2.53e-26	110
MS.gene66476.t1	MTR_1g094215	27.824	363	229	9	45	385	64	415	2.88e-26	110
MS.gene66476.t1	MTR_1g094215	27.824	363	229	9	45	385	247	598	5.10e-26	111
MS.gene66476.t1	MTR_3g096370	29.262	393	244	11	45	413	10	392	7.12e-26	108
MS.gene66476.t1	MTR_7g078330	25.789	380	248	9	45	408	274	635	2.38e-25	109
MS.gene66476.t1	MTR_5g048050	26.434	401	253	10	45	413	25	415	6.47e-25	106
MS.gene66476.t1	MTR_2g062480	65.476	84	9	1	130	213	50	113	5.35e-24	97.1
MS.gene66476.t1	MTR_7g059405	28.358	335	217	10	45	367	264	587	1.24e-22	100
MS.gene66476.t1	MTR_2g018010	27.778	306	194	6	28	312	274	573	1.47e-21	97.8
MS.gene66476.t1	MTR_2g087350	27.094	406	278	11	39	431	1	401	2.87e-20	92.8
MS.gene66476.t1	MTR_7g053260	29.614	233	146	5	46	264	79	307	1.66e-19	90.9
MS.gene66476.t1	MTR_7g116600	31.489	235	140	6	46	264	79	308	3.21e-19	90.1
MS.gene66476.t1	MTR_1g056910	27.470	415	249	18	39	421	1	395	1.39e-18	87.8
MS.gene66476.t1	MTR_5g083030	23.611	360	234	8	44	372	292	641	4.23e-18	87.4
MS.gene66476.t1	MTR_1g056880	26.824	425	261	14	41	421	4	422	7.54e-18	85.9
MS.gene66476.t1	MTR_4g051515	26.253	419	263	16	44	430	5	409	8.19e-18	85.5
MS.gene66476.t1	MTR_4g107010	28.526	312	188	10	46	334	281	580	1.15e-17	85.9
MS.gene66476.t1	MTR_1g056870	26.959	434	249	18	41	421	4	422	1.43e-17	84.7
MS.gene66476.t1	MTR_3g078160	26.590	346	211	10	22	341	254	582	2.32e-17	85.1
MS.gene66476.t1	MTR_3g078160	26.590	346	211	10	22	341	254	582	2.75e-17	84.7
MS.gene66476.t1	MTR_4g028960	29.956	227	115	8	45	237	280	496	9.64e-17	83.2
MS.gene66476.t1	MTR_1g056840	25.768	423	240	14	42	421	5	396	1.14e-16	82.0
MS.gene66476.t1	MTR_4g091880	27.217	327	192	9	44	364	4	290	3.07e-16	80.1
MS.gene66476.t1	MTR_5g034440	27.961	304	191	9	49	333	284	578	6.87e-16	80.5
MS.gene66476.t1	MTR_2g011140	26.149	348	230	11	51	387	21	352	3.37e-15	77.4
MS.gene66476.t1	MTR_3g085610	50.000	66	33	0	46	111	281	346	7.09e-15	77.4
MS.gene66476.t1	MTR_3g078160	28.322	286	176	7	22	285	254	532	1.40e-14	76.3
MS.gene66476.t1	MTR_4g091880	26.979	341	203	12	44	364	4	318	1.94e-14	74.7
MS.gene66476.t1	MTR_1g069845	46.970	66	35	0	46	111	125	190	2.45e-13	72.4
MS.gene66476.t1	MTR_1g093965	47.692	65	34	0	58	122	1	65	3.17e-13	68.6
MS.gene66476.t1	MTR_1g069845	46.970	66	35	0	46	111	246	311	3.56e-13	72.0
MS.gene66476.t1	MTR_1g093995	47.692	65	34	0	58	122	1	65	3.65e-13	68.6
MS.gene66476.t1	MTR_1g079450	25.275	273	181	7	45	303	63	326	3.76e-13	71.2
MS.gene66476.t1	MTR_1g069845	46.970	66	35	0	46	111	242	307	3.91e-13	72.0
MS.gene66476.t1	MTR_3g466220	47.761	67	35	0	45	111	233	299	1.42e-12	70.1
MS.gene66476.t1	MTR_3g466220	47.761	67	35	0	45	111	212	278	1.46e-12	70.1
MS.gene66476.t1	MTR_3g115670	28.452	239	132	12	42	263	27	243	5.53e-12	68.2
MS.gene66476.t1	MTR_8g080280	45.455	66	36	0	46	111	283	348	8.07e-12	67.8
MS.gene66476.t1	MTR_7g097020	45.455	66	35	1	46	111	243	307	1.02e-11	67.4
MS.gene66476.t1	MTR_8g077205	45.455	66	36	0	46	111	164	229	1.14e-11	67.0
MS.gene66476.t1	MTR_8g077205	45.455	66	36	0	46	111	276	341	1.14e-11	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66476.t1	AT3G49810	62.408	407	147	5	28	434	48	448	0.0	516
MS.gene66476.t1	AT5G65920	59.330	418	162	6	19	434	33	444	6.02e-174	494
MS.gene66476.t1	AT5G65920	59.330	418	162	6	19	434	33	444	6.02e-174	494
MS.gene66476.t1	AT3G19380	32.718	379	238	10	36	399	5	381	1.82e-41	152
MS.gene66476.t1	AT1G49780	30.886	395	254	8	36	413	5	397	6.64e-37	140
MS.gene66476.t1	AT3G18710	27.628	409	258	13	37	419	4	400	5.17e-32	126
MS.gene66476.t1	AT5G64660	27.685	419	231	14	45	417	10	402	7.42e-32	126
MS.gene66476.t1	AT3G54850	28.529	340	216	8	45	368	248	576	2.89e-30	124
MS.gene66476.t1	AT1G23030	26.776	366	243	10	26	377	218	572	2.70e-28	117
MS.gene66476.t1	AT2G35930	26.506	415	267	11	39	429	6	406	3.32e-28	115
MS.gene66476.t1	AT3G46510	25.581	387	258	9	45	415	256	628	1.40e-26	113
MS.gene66476.t1	AT1G71020	27.859	341	218	9	45	367	95	425	1.60e-26	111
MS.gene66476.t1	AT1G71020	27.859	341	218	9	45	367	243	573	3.25e-26	111
MS.gene66476.t1	AT1G66160	26.234	385	254	11	45	413	33	403	3.59e-26	110
MS.gene66476.t1	AT5G37490	25.065	387	265	6	45	413	31	410	1.08e-25	108
MS.gene66476.t1	AT5G42340	30.894	246	159	6	45	282	290	532	2.36e-25	109
MS.gene66476.t1	AT2G28830	27.597	308	197	4	46	335	185	484	8.59e-25	107
MS.gene66476.t1	AT5G09800	26.434	401	253	16	42	417	8	391	3.19e-23	101
MS.gene66476.t1	AT3G52450	26.190	420	259	12	39	413	1	414	3.22e-22	99.0
MS.gene66476.t1	AT1G29340	26.370	292	190	5	40	312	300	585	1.93e-20	94.7
MS.gene66476.t1	AT3G07360	28.808	302	185	10	46	331	75	362	1.71e-17	84.7
MS.gene66476.t1	AT5G01830	30.244	205	125	4	44	234	273	473	6.69e-17	83.6
MS.gene66476.t1	AT4G21350	25.076	331	229	7	44	367	4	322	1.17e-16	81.6
MS.gene66476.t1	AT1G67530	54.545	66	30	0	46	111	273	338	1.39e-15	79.3
MS.gene66476.t1	AT1G67530	54.545	66	30	0	46	111	273	338	1.44e-15	79.7
MS.gene66476.t1	AT1G67530	54.545	66	30	0	46	111	273	338	1.44e-15	79.7
MS.gene66476.t1	AT1G67530	54.545	66	30	0	46	111	273	338	1.44e-15	79.7
MS.gene66476.t1	AT1G60190	28.315	279	174	9	51	310	284	555	5.52e-15	77.8
MS.gene66476.t1	AT1G24330	51.515	66	32	0	46	111	276	341	3.49e-14	75.1
MS.gene66476.t1	AT1G24330	51.515	66	32	0	46	111	276	341	3.49e-14	75.1
MS.gene66476.t1	AT3G11840	39.167	120	65	5	44	157	9	126	7.35e-14	73.6
MS.gene66476.t1	AT1G27910	51.515	66	32	0	46	111	280	345	9.48e-14	73.9
MS.gene66476.t1	AT1G10560	24.923	325	200	11	49	339	292	606	6.18e-13	71.2
MS.gene66476.t1	AT2G23140	47.619	63	33	0	49	111	237	299	1.10e-12	70.5
MS.gene66476.t1	AT2G23140	47.619	63	33	0	49	111	234	296	1.14e-12	70.5
MS.gene66476.t1	AT2G23140	47.619	63	33	0	49	111	234	296	1.14e-12	70.5
MS.gene66476.t1	AT2G23140	47.619	63	33	0	49	111	45	107	1.28e-12	70.1
MS.gene66476.t1	AT2G23140	47.619	63	33	0	49	111	45	107	1.28e-12	70.1
MS.gene66476.t1	AT3G54790	42.647	68	39	0	45	112	202	269	2.08e-12	69.7
MS.gene66476.t1	AT3G54790	42.647	68	39	0	45	112	238	305	2.29e-12	69.7
MS.gene66476.t1	AT3G54790	42.647	68	39	0	45	112	238	305	2.29e-12	69.7
MS.gene66476.t1	AT3G02840	28.025	157	107	3	262	412	195	351	7.54e-12	67.0
MS.gene66476.t1	AT5G65500	41.096	73	43	0	43	115	721	793	3.16e-11	65.9
MS.gene66476.t1	AT5G65500	41.096	73	43	0	43	115	713	785	3.23e-11	65.9

Find 68 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTCGTTATTCAAATT+TGG	0.155411	1.4:+5134353	None:intergenic
GACCAAGTGAAAACCATTTA+AGG	0.159560	1.4:+5135059	None:intergenic
TATCGGTATCATTGTGAATT+TGG	0.172886	1.4:-5134711	MS.gene66476:CDS
CATCCAAATGGGATATTAAT+TGG	0.260923	1.4:-5134470	MS.gene66476:CDS
TGTTTCAAGTTTGAGTCTTT+TGG	0.266950	1.4:-5134525	MS.gene66476:CDS
ATAGATCCAACATCCTTAAA+TGG	0.267794	1.4:-5135072	MS.gene66476:CDS
GGTTTGGTTTGTTCTTTGTT+AGG	0.271110	1.4:-5134767	MS.gene66476:CDS
TCAAAGCTCTTAAGGATCTT+AGG	0.281536	1.4:-5134847	MS.gene66476:CDS
ATTGATTGAAATGTTGTTGA+TGG	0.296284	1.4:-5134579	MS.gene66476:CDS
GCCTTTGAAGCACATTCTTC+AGG	0.321820	1.4:+5134161	None:intergenic
TGAAATGTTGTTGATGGAAT+TGG	0.325557	1.4:-5134573	MS.gene66476:CDS
GAAGATTTCTGTTTCAAAAC+AGG	0.331345	1.4:+5134083	None:intergenic
AACACTTGAAGAAGGGAAAT+TGG	0.332578	1.4:-5134297	MS.gene66476:CDS
CATAACTCTTGCATTGTTGT+TGG	0.349959	1.4:+5135025	None:intergenic
GGAAAATGGCGGGGTTGGTT+TGG	0.353204	1.4:-5134783	MS.gene66476:CDS
TGCATTGGTTCAAGTGAGAT+TGG	0.353424	1.4:+5135130	None:intergenic
GGATGTTCAAGGAAGAGTTT+TGG	0.360543	1.4:-5134912	MS.gene66476:CDS
CAACAACAATGCAAGAGTTA+TGG	0.362659	1.4:-5135024	MS.gene66476:CDS
AGACCAATTAATATCCCATT+TGG	0.368703	1.4:+5134467	None:intergenic
AAGGATGTTGGATCTATCAT+AGG	0.371646	1.4:+5135078	None:intergenic
AAATTTGAATAACGAGTGTT+TGG	0.375150	1.4:-5134351	MS.gene66476:CDS
TGACCTGTGCAAAGTGTAAC+AGG	0.377472	1.4:+5135103	None:intergenic
GCATCCAGCTAAAGTTTCAT+TGG	0.383621	1.4:-5134654	MS.gene66476:CDS
AGGAAAACAGTGTTGGAAAA+TGG	0.387358	1.4:-5134797	MS.gene66476:CDS
AACAACAATGCAAGAGTTAT+GGG	0.388335	1.4:-5135023	MS.gene66476:CDS
TGAGATTAATAATCCTTTGT+TGG	0.392736	1.4:-5135272	MS.gene66476:CDS
GATAGCACTTTACATATTAG+AGG	0.398032	1.4:-5134327	MS.gene66476:CDS
GAGATTAATAATCCTTTGTT+GGG	0.398485	1.4:-5135271	MS.gene66476:CDS
ACCACATTAGGTATAATGTT+TGG	0.400820	1.4:+5134260	None:intergenic
AAAGGCTGTGGAAGCTGGAT+TGG	0.406186	1.4:-5134144	MS.gene66476:CDS
ATCAACATCTTTACCACATT+AGG	0.406347	1.4:+5134248	None:intergenic
AAGTAGAGTCAAAGCTCTTA+AGG	0.410312	1.4:-5134855	MS.gene66476:CDS
TGAAAACCATTTAAGGATGT+TGG	0.416261	1.4:+5135066	None:intergenic
TTTGGTTGGATTATTGAGAT+TGG	0.418112	1.4:-5134507	MS.gene66476:CDS
GCTTCAAAGGCTGTGGAAGC+TGG	0.429451	1.4:-5134149	MS.gene66476:CDS
CAATTGTTTGAGAGTGTTGT+TGG	0.445240	1.4:+5134985	None:intergenic
AGAGATAAGAAACATCCAAA+TGG	0.458686	1.4:-5134482	MS.gene66476:CDS
TCAAACAATTGATCTTTACT+TGG	0.459813	1.4:-5134973	MS.gene66476:CDS
TTGTTTCTCATGTTTGTGCT+AGG	0.464535	1.4:-5134817	MS.gene66476:CDS
TGGTTCTCACACAAGTACTT+AGG	0.487678	1.4:-5134953	MS.gene66476:CDS
GTTGTTGATATTATGAATGA+AGG	0.490566	1.4:-5134632	MS.gene66476:CDS
AATTGTTTGAGAGTGTTGTT+GGG	0.500960	1.4:+5134986	None:intergenic
GAGATAAGAAACATCCAAAT+GGG	0.501701	1.4:-5134481	MS.gene66476:CDS
GAGACACTCAAGAAGGTAAA+GGG	0.502037	1.4:-5134881	MS.gene66476:CDS
ATTGGACATATAAACACATA+AGG	0.504104	1.4:+5135148	None:intergenic
AAACAGTGTTGGAAAATGGC+GGG	0.518645	1.4:-5134793	MS.gene66476:CDS
AAGAAGCTTGAGGATGTTCA+AGG	0.520273	1.4:-5134923	MS.gene66476:CDS
GTGTTGGAAAATGGCGGGGT+TGG	0.525268	1.4:-5134788	MS.gene66476:CDS
ACAACCAATGAAACTTTAGC+TGG	0.528214	1.4:+5134650	None:intergenic
GGAGACACTCAAGAAGGTAA+AGG	0.529677	1.4:-5134882	MS.gene66476:CDS
AGTGTAACAGGCTCTTGCAT+TGG	0.532871	1.4:+5135115	None:intergenic
GGTAAAATCTCAATCAATTG+CGG	0.551711	1.4:+5134374	None:intergenic
ACCTGAAGAATGTGCTTCAA+AGG	0.554133	1.4:-5134162	MS.gene66476:CDS
AGAATGTGCTTCAAAGGCTG+TGG	0.559930	1.4:-5134156	MS.gene66476:CDS
TCCAAACATTATACCTAATG+TGG	0.567506	1.4:-5134261	MS.gene66476:CDS
GTATTGTCAACACTTGAAGA+AGG	0.568213	1.4:-5134305	MS.gene66476:CDS
GAGCCTGTTACACTTTGCAC+AGG	0.589911	1.4:-5135106	MS.gene66476:CDS
AGAAGCTCTATGATAAGCAT+AGG	0.590919	1.4:-5134404	MS.gene66476:CDS
GATGTTTCAAAACCCAACAA+AGG	0.598829	1.4:+5135259	None:intergenic
TTGTGCTAGGAAAACAGTGT+TGG	0.603588	1.4:-5134804	MS.gene66476:CDS
TATTGTCAACACTTGAAGAA+GGG	0.604875	1.4:-5134304	MS.gene66476:CDS
AACATTGGAGACACTCAAGA+AGG	0.620353	1.4:-5134888	MS.gene66476:CDS
AACAGTGTTGGAAAATGGCG+GGG	0.621402	1.4:-5134792	MS.gene66476:CDS
GTTGTTGGTTGTGAAGCTAT+CGG	0.633853	1.4:-5134728	MS.gene66476:CDS
AAAACAGTGTTGGAAAATGG+CGG	0.637218	1.4:-5134794	MS.gene66476:CDS
AGGAATGAAGAAGAAGCTTG+AGG	0.637995	1.4:-5134933	MS.gene66476:CDS
ATGTTGTTGATGGAATTGGA+AGG	0.713581	1.4:-5134569	MS.gene66476:CDS
TTATTGCTTGTGATTCAGAG+TGG	0.747357	1.4:-5134113	MS.gene66476:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACACTCGTTATTCAAATT+TGG	+	chr1.4:5134934-5134953	None:intergenic	25.0%
!	AAATTTGAATAACGAGTGTT+TGG	-	chr1.4:5134933-5134952	MS.gene66476:CDS	25.0%
!	ATTGATTGAAATGTTGTTGA+TGG	-	chr1.4:5134705-5134724	MS.gene66476:CDS	25.0%
!	ATTGGACATATAAACACATA+AGG	+	chr1.4:5134139-5134158	None:intergenic	25.0%
!	GAGATTAATAATCCTTTGTT+GGG	-	chr1.4:5134013-5134032	MS.gene66476:CDS	25.0%
!	GTTGTTGATATTATGAATGA+AGG	-	chr1.4:5134652-5134671	MS.gene66476:CDS	25.0%
!	TCAAACAATTGATCTTTACT+TGG	-	chr1.4:5134311-5134330	MS.gene66476:CDS	25.0%
!	TGAGATTAATAATCCTTTGT+TGG	-	chr1.4:5134012-5134031	MS.gene66476:CDS	25.0%
!!	AATTTGCACTGTCAATTTTA+TGG	-	chr1.4:5135082-5135101	MS.gene66476:CDS	25.0%
!!	ATTTGCACTGTCAATTTTAT+GGG	-	chr1.4:5135083-5135102	MS.gene66476:CDS	25.0%
	AACAACAATGCAAGAGTTAT+GGG	-	chr1.4:5134261-5134280	MS.gene66476:CDS	30.0%
	ACCACATTAGGTATAATGTT+TGG	+	chr1.4:5135027-5135046	None:intergenic	30.0%
	AGACCAATTAATATCCCATT+TGG	+	chr1.4:5134820-5134839	None:intergenic	30.0%
	AGAGATAAGAAACATCCAAA+TGG	-	chr1.4:5134802-5134821	MS.gene66476:CDS	30.0%
	ATAGATCCAACATCCTTAAA+TGG	-	chr1.4:5134212-5134231	MS.gene66476:CDS	30.0%
	ATCAACATCTTTACCACATT+AGG	+	chr1.4:5135039-5135058	None:intergenic	30.0%
	CATCCAAATGGGATATTAAT+TGG	-	chr1.4:5134814-5134833	MS.gene66476:CDS	30.0%
	GAAGATTTCTGTTTCAAAAC+AGG	+	chr1.4:5135204-5135223	None:intergenic	30.0%
	GAGATAAGAAACATCCAAAT+GGG	-	chr1.4:5134803-5134822	MS.gene66476:CDS	30.0%
	GATAGCACTTTACATATTAG+AGG	-	chr1.4:5134957-5134976	MS.gene66476:CDS	30.0%
	GGTAAAATCTCAATCAATTG+CGG	+	chr1.4:5134913-5134932	None:intergenic	30.0%
	TATCGGTATCATTGTGAATT+TGG	-	chr1.4:5134573-5134592	MS.gene66476:CDS	30.0%
	TATTGTCAACACTTGAAGAA+GGG	-	chr1.4:5134980-5134999	MS.gene66476:CDS	30.0%
	TCCAAACATTATACCTAATG+TGG	-	chr1.4:5135023-5135042	MS.gene66476:CDS	30.0%
	TGAAAACCATTTAAGGATGT+TGG	+	chr1.4:5134221-5134240	None:intergenic	30.0%
	TTTGGTTGGATTATTGAGAT+TGG	-	chr1.4:5134777-5134796	MS.gene66476:CDS	30.0%
!	ATCCTTAAATGGTTTTCACT+TGG	-	chr1.4:5134223-5134242	MS.gene66476:CDS	30.0%
!	TCTTTTACTTCACATGTTGT+TGG	-	chr1.4:5134541-5134560	MS.gene66476:CDS	30.0%
!	TGAAATGTTGTTGATGGAAT+TGG	-	chr1.4:5134711-5134730	MS.gene66476:CDS	30.0%
!!	AATTGTTTGAGAGTGTTGTT+GGG	+	chr1.4:5134301-5134320	None:intergenic	30.0%
!!!	GGTTTTGAAACATCTTTCAA+AGG	-	chr1.4:5134034-5134053	MS.gene66476:CDS	30.0%
!!!	TGTTTCAAGTTTGAGTCTTT+TGG	-	chr1.4:5134759-5134778	MS.gene66476:CDS	30.0%
	AACACTTGAAGAAGGGAAAT+TGG	-	chr1.4:5134987-5135006	MS.gene66476:CDS	35.0%
	AAGGATGTTGGATCTATCAT+AGG	+	chr1.4:5134209-5134228	None:intergenic	35.0%
	AAGTAGAGTCAAAGCTCTTA+AGG	-	chr1.4:5134429-5134448	MS.gene66476:CDS	35.0%
	ACAACCAATGAAACTTTAGC+TGG	+	chr1.4:5134637-5134656	None:intergenic	35.0%
	AGAAGCTCTATGATAAGCAT+AGG	-	chr1.4:5134880-5134899	MS.gene66476:CDS	35.0%
	CAACAACAATGCAAGAGTTA+TGG	-	chr1.4:5134260-5134279	MS.gene66476:CDS	35.0%
	CATAACTCTTGCATTGTTGT+TGG	+	chr1.4:5134262-5134281	None:intergenic	35.0%
	GACCAAGTGAAAACCATTTA+AGG	+	chr1.4:5134228-5134247	None:intergenic	35.0%
	GATGTTTCAAAACCCAACAA+AGG	+	chr1.4:5134028-5134047	None:intergenic	35.0%
	GTATTGTCAACACTTGAAGA+AGG	-	chr1.4:5134979-5134998	MS.gene66476:CDS	35.0%
	TCAAAGCTCTTAAGGATCTT+AGG	-	chr1.4:5134437-5134456	MS.gene66476:CDS	35.0%
	TTATTGCTTGTGATTCAGAG+TGG	-	chr1.4:5135171-5135190	MS.gene66476:CDS	35.0%
!	AAAACAGTGTTGGAAAATGG+CGG	-	chr1.4:5134490-5134509	MS.gene66476:CDS	35.0%
!	AGGAAAACAGTGTTGGAAAA+TGG	-	chr1.4:5134487-5134506	MS.gene66476:CDS	35.0%
!	ATGTTGTTGATGGAATTGGA+AGG	-	chr1.4:5134715-5134734	MS.gene66476:CDS	35.0%
!	TCAAGTTTGAGTCTTTTGGT+TGG	-	chr1.4:5134763-5134782	MS.gene66476:CDS	35.0%
!	TTGTTTCTCATGTTTGTGCT+AGG	-	chr1.4:5134467-5134486	MS.gene66476:CDS	35.0%
!!	CAATTGTTTGAGAGTGTTGT+TGG	+	chr1.4:5134302-5134321	None:intergenic	35.0%
!!	GGTTTGGTTTGTTCTTTGTT+AGG	-	chr1.4:5134517-5134536	MS.gene66476:CDS	35.0%
!!!	AGGAAGAGTTTTGGAAACAT+TGG	-	chr1.4:5134381-5134400	MS.gene66476:CDS	35.0%
	AACATTGGAGACACTCAAGA+AGG	-	chr1.4:5134396-5134415	MS.gene66476:CDS	40.0%
	AAGAAGCTTGAGGATGTTCA+AGG	-	chr1.4:5134361-5134380	MS.gene66476:CDS	40.0%
	ACCTGAAGAATGTGCTTCAA+AGG	-	chr1.4:5135122-5135141	MS.gene66476:CDS	40.0%
	AGGAATGAAGAAGAAGCTTG+AGG	-	chr1.4:5134351-5134370	MS.gene66476:CDS	40.0%
	GAGACACTCAAGAAGGTAAA+GGG	-	chr1.4:5134403-5134422	MS.gene66476:CDS	40.0%
	GCATCCAGCTAAAGTTTCAT+TGG	-	chr1.4:5134630-5134649	MS.gene66476:CDS	40.0%
	TGGTTCTCACACAAGTACTT+AGG	-	chr1.4:5134331-5134350	MS.gene66476:CDS	40.0%
	TTGTGCTAGGAAAACAGTGT+TGG	-	chr1.4:5134480-5134499	MS.gene66476:CDS	40.0%
!	AAACAGTGTTGGAAAATGGC+GGG	-	chr1.4:5134491-5134510	MS.gene66476:CDS	40.0%
!	GGATGTTCAAGGAAGAGTTT+TGG	-	chr1.4:5134372-5134391	MS.gene66476:CDS	40.0%
!	GTTGTTGGTTGTGAAGCTAT+CGG	-	chr1.4:5134556-5134575	MS.gene66476:CDS	40.0%
!!	TGCATTGGTTCAAGTGAGAT+TGG	+	chr1.4:5134157-5134176	None:intergenic	40.0%
	AGAATGTGCTTCAAAGGCTG+TGG	-	chr1.4:5135128-5135147	MS.gene66476:CDS	45.0%
	AGTGTAACAGGCTCTTGCAT+TGG	+	chr1.4:5134172-5134191	None:intergenic	45.0%
	GGAGACACTCAAGAAGGTAA+AGG	-	chr1.4:5134402-5134421	MS.gene66476:CDS	45.0%
	TGACCTGTGCAAAGTGTAAC+AGG	+	chr1.4:5134184-5134203	None:intergenic	45.0%
!	AACAGTGTTGGAAAATGGCG+GGG	-	chr1.4:5134492-5134511	MS.gene66476:CDS	45.0%
!!	GCCTTTGAAGCACATTCTTC+AGG	+	chr1.4:5135126-5135145	None:intergenic	45.0%
	AAAGGCTGTGGAAGCTGGAT+TGG	-	chr1.4:5135140-5135159	MS.gene66476:CDS	50.0%
	GAGCCTGTTACACTTTGCAC+AGG	-	chr1.4:5134178-5134197	MS.gene66476:CDS	50.0%
	GCTTCAAAGGCTGTGGAAGC+TGG	-	chr1.4:5135135-5135154	MS.gene66476:CDS	55.0%
!	GTGTTGGAAAATGGCGGGGT+TGG	-	chr1.4:5134496-5134515	MS.gene66476:CDS	55.0%
!!	GGAAAATGGCGGGGTTGGTT+TGG	-	chr1.4:5134501-5134520	MS.gene66476:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	5134001	5135305	5134001	ID=MS.gene66476
chr1.4	mRNA	5134001	5135305	5134001	ID=MS.gene66476.t1;Parent=MS.gene66476
chr1.4	exon	5134001	5135305	5134001	ID=MS.gene66476.t1.exon1;Parent=MS.gene66476.t1
chr1.4	CDS	5134001	5135305	5134001	ID=cds.MS.gene66476.t1;Parent=MS.gene66476.t1

Gene Sequence

>MS.gene66476

ATGCTTTCATATGAGATTAATAATCCTTTGTTGGGTTTTGAAACATCTTTCAAAGGTTGTTTTTTTAGTGTCTTAGACACTGAAAAAATAGATCTCAAGTTGATAATTGAAGAGCTTGATTCAATTGATGATGTTCCTTATGTGTTTATATGTCCAATCTCACTTGAACCAATGCAAGAGCCTGTTACACTTTGCACAGGTCAAACCTATGATAGATCCAACATCCTTAAATGGTTTTCACTTGGTCACAAAACATGTCCAACAACAATGCAAGAGTTATGGGATGATTCAATCACTCCCAACAACACTCTCAAACAATTGATCTTTACTTGGTTCTCACACAAGTACTTAGGAATGAAGAAGAAGCTTGAGGATGTTCAAGGAAGAGTTTTGGAAACATTGGAGACACTCAAGAAGGTAAAGGGTCAAAGTAGAGTCAAAGCTCTTAAGGATCTTAGGAAACTTGTTGTTTCTCATGTTTGTGCTAGGAAAACAGTGTTGGAAAATGGCGGGGTTGGTTTGGTTTGTTCTTTGTTAGGTTCTTTTACTTCACATGTTGTTGGTTGTGAAGCTATCGGTATCATTGTGAATTTGGATTTGAGTTTAGAATTGAAGAGAAAGTATCTTATGCATCCAGCTAAAGTTTCATTGGTTGTTGATATTATGAATGAAGGAACTATTGAAACAAAGATGAATTGTGCTAAATTGATTGAAATGTTGTTGATGGAATTGGAAGGAAATGAAACTATGATTGAGATTGTTTCAAGTTTGAGTCTTTTGGTTGGATTATTGAGATTGGTGAGAGATAAGAAACATCCAAATGGGATATTAATTGGTCTCACATTGCTTAAAAAAGTATTATGTTGTCATGAATCAATTAGAAGCTCTATGATAAGCATAGGAGCAATTCCGCAATTGATTGAGATTTTACCAAATTTGAATAACGAGTGTTTGGAGATAGCACTTTACATATTAGAGGTATTGTCAACACTTGAAGAAGGGAAATTGGCATTGAAAGAATGTCCAAACATTATACCTAATGTGGTAAAGATGTTGATGAGAGTCTCAGAAAATTGTACACAATTTGCACTGTCAATTTTATGGGCTATTTACAAGCTTGCACCTGAAGAATGTGCTTCAAAGGCTGTGGAAGCTGGATTGGCTTCAAAATTATTGCTTGTGATTCAGAGTGGTTGTAATCCTGTTTTGAAACAGAAATCTTCAGAGTTTTTGAAAATGTGTAGTGTGAATTATTCTACTAGTATTTTTATTTCTAAGTGTATGCTTACTACAACAATACAATGA

Protein sequence

>MS.gene66476.t1

MLSYEINNPLLGFETSFKGCFFSVLDTEKIDLKLIIEELDSIDDVPYVFICPISLEPMQEPVTLCTGQTYDRSNILKWFSLGHKTCPTTMQELWDDSITPNNTLKQLIFTWFSHKYLGMKKKLEDVQGRVLETLETLKKVKGQSRVKALKDLRKLVVSHVCARKTVLENGGVGLVCSLLGSFTSHVVGCEAIGIIVNLDLSLELKRKYLMHPAKVSLVVDIMNEGTIETKMNCAKLIEMLLMELEGNETMIEIVSSLSLLVGLLRLVRDKKHPNGILIGLTLLKKVLCCHESIRSSMISIGAIPQLIEILPNLNNECLEIALYILEVLSTLEEGKLALKECPNIIPNVVKMLMRVSENCTQFALSILWAIYKLAPEECASKAVEAGLASKLLLVIQSGCNPVLKQKSSEFLKMCSVNYSTSIFISKCMLTTTIQ