Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67105.t1 | XP_003589223.1 | 94.7 | 208 | 11 | 0 | 1 | 208 | 1 | 208 | 1.60E-110 | 408.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67105.t1 | G7I4F7 | 94.7 | 208 | 11 | 0 | 1 | 208 | 1 | 208 | 1.2e-110 | 408.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene67105 | 0.800082 | 1.70E-48 | -1.69E-46 |
| MS.gene049501 | MS.gene67105 | 0.81025 | 1.27E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene67105 | 0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene67105 | 0.80979 | 1.59E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene67105 | 0.816905 | 4.35E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene67105 | 0.827479 | 1.53E-54 | -1.69E-46 |
| MS.gene050680 | MS.gene67105 | 0.823407 | 1.41E-53 | -1.69E-46 |
| MS.gene050750 | MS.gene67105 | 0.813551 | 2.42E-51 | -1.69E-46 |
| MS.gene050787 | MS.gene67105 | 0.817917 | 2.57E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene67105 | 0.801524 | 8.64E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene67105 | 0.801875 | 7.32E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene67105 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene051265 | MS.gene67105 | 0.851969 | 6.28E-61 | -1.69E-46 |
| MS.gene052190 | MS.gene67105 | 0.800962 | 1.13E-48 | -1.69E-46 |
| MS.gene052333 | MS.gene67105 | 0.802764 | 4.80E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene67105 | 0.821371 | 4.18E-53 | -1.69E-46 |
| MS.gene053184 | MS.gene67105 | 0.838367 | 3.00E-57 | -1.69E-46 |
| MS.gene053494 | MS.gene67105 | 0.802565 | 5.28E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene67105 | 0.818155 | 2.27E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene67105 | -0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene05434 | MS.gene67105 | 0.810854 | 9.37E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene67105 | 0.818317 | 2.09E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene67105 | 0.828291 | 9.76E-55 | -1.69E-46 |
| MS.gene054542 | MS.gene67105 | 0.804644 | 1.96E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene67105 | 0.82389 | 1.09E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene67105 | 0.800176 | 1.63E-48 | -1.69E-46 |
| MS.gene05510 | MS.gene67105 | 0.833997 | 3.87E-56 | -1.69E-46 |
| MS.gene055356 | MS.gene67105 | 0.834465 | 2.95E-56 | -1.69E-46 |
| MS.gene055357 | MS.gene67105 | 0.806362 | 8.53E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene67105 | 0.838153 | 3.41E-57 | -1.69E-46 |
| MS.gene055701 | MS.gene67105 | 0.835315 | 1.80E-56 | -1.69E-46 |
| MS.gene055804 | MS.gene67105 | 0.820328 | 7.27E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene67105 | 0.810131 | 1.34E-50 | -1.69E-46 |
| MS.gene055926 | MS.gene67105 | 0.8022 | 6.28E-49 | -1.69E-46 |
| MS.gene057194 | MS.gene67105 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene67105 | 0.80767 | 4.51E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene67105 | 0.808409 | 3.14E-50 | -1.69E-46 |
| MS.gene059124 | MS.gene67105 | 0.811282 | 7.57E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene67105 | 0.84494 | 5.54E-59 | -1.69E-46 |
| MS.gene059161 | MS.gene67105 | 0.802933 | 4.43E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene67105 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene67105 | 0.803149 | 4.00E-49 | -1.69E-46 |
| MS.gene060137 | MS.gene67105 | -0.807981 | 3.88E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene67105 | 0.863026 | 3.34E-64 | -1.69E-46 |
| MS.gene060802 | MS.gene67105 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene67105 | 0.807072 | 6.04E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene67105 | 0.811617 | 6.40E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene67105 | -0.808485 | 3.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67105.t1 | MTR_1g019800 | 94.712 | 208 | 11 | 0 | 1 | 208 | 1 | 208 | 3.46e-147 | 407 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67105.t1 | AT1G47410 | 38.000 | 200 | 88 | 6 | 21 | 208 | 9 | 184 | 7.52e-32 | 114 |
| MS.gene67105.t1 | AT1G47410 | 38.000 | 200 | 88 | 6 | 21 | 208 | 9 | 184 | 7.52e-32 | 114 |
Find 37 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCATCAAGATCACTAAT+TGG | 0.271099 | 1.3:+3822783 | None:intergenic |
| TTGGAAGCGTTCTATAATTC+AGG | 0.285690 | 1.3:-3822299 | MS.gene67105:CDS |
| TTACCTCCCTCCTTTGCCTT+TGG | 0.326286 | 1.3:+3822469 | None:intergenic |
| GTGTATTCTTCTAGTGCTAA+TGG | 0.339241 | 1.3:-3822394 | MS.gene67105:CDS |
| TGCAAAGCATTTGCATCTCT+TGG | 0.342800 | 1.3:+3822890 | None:intergenic |
| ACAAGAGAATTATGTGTTAC+TGG | 0.345937 | 1.3:-3822502 | MS.gene67105:CDS |
| CATTGTGAAGGATGGCCTTT+CGG | 0.363378 | 1.3:-3822268 | MS.gene67105:CDS |
| ACACATAATTCTCTTGTTGC+AGG | 0.374763 | 1.3:+3822508 | None:intergenic |
| TGTATTCTTCTAGTGCTAAT+GGG | 0.374850 | 1.3:-3822393 | MS.gene67105:CDS |
| TTGCATCTCTTGGAATGGTT+TGG | 0.387372 | 1.3:+3822900 | None:intergenic |
| CATATAAATTCAAGTAACTT+TGG | 0.429792 | 1.3:+3822162 | None:intergenic |
| TAGTGATCTTGATGAAGAAC+AGG | 0.434961 | 1.3:-3822778 | MS.gene67105:CDS |
| ATTCAAGTAACTTTGGTACT+TGG | 0.457278 | 1.3:+3822169 | None:intergenic |
| ATGCTTTGCAGCTGCTCTGA+AGG | 0.476673 | 1.3:-3822877 | MS.gene67105:CDS |
| TAATTGGAAACTCCTCTTCA+AGG | 0.478540 | 1.3:+3822799 | None:intergenic |
| GGTTTGGTTGCTCCTCCTCA+TGG | 0.490726 | 1.3:+3822916 | None:intergenic |
| GGCGGTTAGAAGCCGAGCTA+TGG | 0.496315 | 1.3:-3822587 | MS.gene67105:CDS |
| AAACTCTCTCTCAGTAGGCT+TGG | 0.513974 | 1.3:+3822547 | None:intergenic |
| CTAAGTCACCATCTGAATCA+TGG | 0.518553 | 1.3:-3822210 | MS.gene67105:CDS |
| AGCATTTGCATCTCTTGGAA+TGG | 0.526952 | 1.3:+3822895 | None:intergenic |
| TTCGACCAAAAGTTTGACAC+TGG | 0.545482 | 1.3:+3822844 | None:intergenic |
| TCTTCTAGTGCTAATGGGGA+AGG | 0.547226 | 1.3:-3822388 | MS.gene67105:CDS |
| TCAAGCTTGAACATCTTGAA+AGG | 0.551732 | 1.3:+3822344 | None:intergenic |
| AATGGAACCAAAGGCAAAGG+AGG | 0.558037 | 1.3:-3822476 | MS.gene67105:CDS |
| TTCGACTACAAGCCTTGAAG+AGG | 0.565475 | 1.3:-3822811 | MS.gene67105:CDS |
| CGATACAACCATGATTCAGA+TGG | 0.575454 | 1.3:+3822202 | None:intergenic |
| CATGATTCAGATGGTGACTT+AGG | 0.584417 | 1.3:+3822211 | None:intergenic |
| TCGACCAAAAGTTTGACACT+GGG | 0.608137 | 1.3:+3822845 | None:intergenic |
| GTATTCTTCTAGTGCTAATG+GGG | 0.608962 | 1.3:-3822392 | MS.gene67105:CDS |
| GATGGTGACTTAGGCAGTGG+TGG | 0.623202 | 1.3:+3822220 | None:intergenic |
| ATGGAACCAAAGGCAAAGGA+GGG | 0.631049 | 1.3:-3822475 | MS.gene67105:CDS |
| GCTTTACGACAGAGACCGAA+AGG | 0.645910 | 1.3:+3822253 | None:intergenic |
| GAGGGAGGTAATGAAGAACA+AGG | 0.652290 | 1.3:-3822457 | MS.gene67105:CDS |
| TCAGATGGTGACTTAGGCAG+TGG | 0.663320 | 1.3:+3822217 | None:intergenic |
| GAACCAAAGGCAAAGGAGGG+AGG | 0.663364 | 1.3:-3822472 | MS.gene67105:CDS |
| TCTTCAGGTGATTGTCTCGG+CGG | 0.664433 | 1.3:-3822605 | MS.gene67105:intron |
| TGTTGCAGGAGAATATACCC+AGG | 0.714302 | 1.3:+3822522 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAAAATAGTTTGATTTTA+AGG | + | chr1.3:3822380-3822399 | None:intergenic | 15.0% |
| !! | AATTAGTTATCGTTCTTTTG+TGG | - | chr1.3:3822470-3822489 | MS.gene67105:CDS | 25.0% |
| !! | ATTATGTGTTACTGGAAAAA+TGG | - | chr1.3:3822607-3822626 | MS.gene67105:intron | 25.0% |
| !!! | ATTTTGATTTGTCGGAATAT+TGG | - | chr1.3:3822783-3822802 | MS.gene67105:CDS | 25.0% |
| !!! | CTTGAATGATTTTGATTTGT+CGG | - | chr1.3:3822775-3822794 | MS.gene67105:intron | 25.0% |
| !!! | TAGTTTGATTTTAAGGTTGA+AGG | + | chr1.3:3822373-3822392 | None:intergenic | 25.0% |
| ACAAGAGAATTATGTGTTAC+TGG | - | chr1.3:3822599-3822618 | MS.gene67105:CDS | 30.0% | |
| TCTTCATCAAGATCACTAAT+TGG | + | chr1.3:3822321-3822340 | None:intergenic | 30.0% | |
| TGTATTCTTCTAGTGCTAAT+GGG | - | chr1.3:3822708-3822727 | MS.gene67105:intron | 30.0% | |
| ! | TGAAAGAGAAGAGTTTTTGT+CGG | - | chr1.3:3822667-3822686 | MS.gene67105:intron | 30.0% |
| AAAAATGGAACCAAAGGCAA+AGG | - | chr1.3:3822622-3822641 | MS.gene67105:intron | 35.0% | |
| ACACATAATTCTCTTGTTGC+AGG | + | chr1.3:3822596-3822615 | None:intergenic | 35.0% | |
| GTATTCTTCTAGTGCTAATG+GGG | - | chr1.3:3822709-3822728 | MS.gene67105:intron | 35.0% | |
| GTGTATTCTTCTAGTGCTAA+TGG | - | chr1.3:3822707-3822726 | MS.gene67105:intron | 35.0% | |
| TAATTGGAAACTCCTCTTCA+AGG | + | chr1.3:3822305-3822324 | None:intergenic | 35.0% | |
| TACTGGAAAAATGGAACCAA+AGG | - | chr1.3:3822616-3822635 | MS.gene67105:intron | 35.0% | |
| TAGTGATCTTGATGAAGAAC+AGG | - | chr1.3:3822323-3822342 | MS.gene67105:CDS | 35.0% | |
| TCAAGCTTGAACATCTTGAA+AGG | + | chr1.3:3822760-3822779 | None:intergenic | 35.0% | |
| ! | GTTCTTTTGTGGTTTTCTTC+AGG | - | chr1.3:3822481-3822500 | MS.gene67105:CDS | 35.0% |
| ! | TTTTCCCAGTGTCAAACTTT+TGG | - | chr1.3:3822252-3822271 | MS.gene67105:CDS | 35.0% |
| ! | TTTTCTTCAGGTGATTGTCT+CGG | - | chr1.3:3822493-3822512 | MS.gene67105:CDS | 35.0% |
| !! | AGTTTTGTCATTGTGAAGGA+TGG | - | chr1.3:3822825-3822844 | MS.gene67105:CDS | 35.0% |
| !! | TCTTTTGTTTCTCCATAGCT+CGG | + | chr1.3:3822529-3822548 | None:intergenic | 35.0% |
| !! | TTGGAAGCGTTCTATAATTC+AGG | - | chr1.3:3822802-3822821 | MS.gene67105:CDS | 35.0% |
| AGCATTTGCATCTCTTGGAA+TGG | + | chr1.3:3822209-3822228 | None:intergenic | 40.0% | |
| AGGAAAAACTCTCTCTCAGT+AGG | + | chr1.3:3822562-3822581 | None:intergenic | 40.0% | |
| CATGATTCAGATGGTGACTT+AGG | + | chr1.3:3822893-3822912 | None:intergenic | 40.0% | |
| CGATACAACCATGATTCAGA+TGG | + | chr1.3:3822902-3822921 | None:intergenic | 40.0% | |
| CTAAGTCACCATCTGAATCA+TGG | - | chr1.3:3822891-3822910 | MS.gene67105:CDS | 40.0% | |
| TCGACCAAAAGTTTGACACT+GGG | + | chr1.3:3822259-3822278 | None:intergenic | 40.0% | |
| TTCGACCAAAAGTTTGACAC+TGG | + | chr1.3:3822260-3822279 | None:intergenic | 40.0% | |
| TTGCATCTCTTGGAATGGTT+TGG | + | chr1.3:3822204-3822223 | None:intergenic | 40.0% | |
| ! | AAACTTTTGGTCGAAGTCGA+AGG | - | chr1.3:3822265-3822284 | MS.gene67105:CDS | 40.0% |
| ! | ACTGAGAGAGAGTTTTTCCT+GGG | - | chr1.3:3822562-3822581 | MS.gene67105:CDS | 40.0% |
| ! | TACTGAGAGAGAGTTTTTCC+TGG | - | chr1.3:3822561-3822580 | MS.gene67105:CDS | 40.0% |
| ! | TGCAAAGCATTTGCATCTCT+TGG | + | chr1.3:3822214-3822233 | None:intergenic | 40.0% |
| !! | CAGGAGTTTTGTCATTGTGA+AGG | - | chr1.3:3822821-3822840 | MS.gene67105:CDS | 40.0% |
| AAACTCTCTCTCAGTAGGCT+TGG | + | chr1.3:3822557-3822576 | None:intergenic | 45.0% | |
| AATGGAACCAAAGGCAAAGG+AGG | - | chr1.3:3822625-3822644 | MS.gene67105:intron | 45.0% | |
| ATGGAACCAAAGGCAAAGGA+GGG | - | chr1.3:3822626-3822645 | MS.gene67105:intron | 45.0% | |
| CATTGTGAAGGATGGCCTTT+CGG | - | chr1.3:3822833-3822852 | MS.gene67105:CDS | 45.0% | |
| GAGGGAGGTAATGAAGAACA+AGG | - | chr1.3:3822644-3822663 | MS.gene67105:intron | 45.0% | |
| TCTTCTAGTGCTAATGGGGA+AGG | - | chr1.3:3822713-3822732 | MS.gene67105:intron | 45.0% | |
| TGTTGCAGGAGAATATACCC+AGG | + | chr1.3:3822582-3822601 | None:intergenic | 45.0% | |
| TTCGACTACAAGCCTTGAAG+AGG | - | chr1.3:3822290-3822309 | MS.gene67105:CDS | 45.0% | |
| ATGCTTTGCAGCTGCTCTGA+AGG | - | chr1.3:3822224-3822243 | MS.gene67105:CDS | 50.0% | |
| GCTTTACGACAGAGACCGAA+AGG | + | chr1.3:3822851-3822870 | None:intergenic | 50.0% | |
| TCAGATGGTGACTTAGGCAG+TGG | + | chr1.3:3822887-3822906 | None:intergenic | 50.0% | |
| TCTTCAGGTGATTGTCTCGG+CGG | - | chr1.3:3822496-3822515 | MS.gene67105:CDS | 50.0% | |
| TTACCTCCCTCCTTTGCCTT+TGG | + | chr1.3:3822635-3822654 | None:intergenic | 50.0% | |
| ! | GGGTGTTTTCTACCATGAGG+AGG | - | chr1.3:3822173-3822192 | MS.gene67105:CDS | 50.0% |
| GAACCAAAGGCAAAGGAGGG+AGG | - | chr1.3:3822629-3822648 | MS.gene67105:intron | 55.0% | |
| GATGGTGACTTAGGCAGTGG+TGG | + | chr1.3:3822884-3822903 | None:intergenic | 55.0% | |
| ! | GGTTTGGTTGCTCCTCCTCA+TGG | + | chr1.3:3822188-3822207 | None:intergenic | 55.0% |
| GGCGGTTAGAAGCCGAGCTA+TGG | - | chr1.3:3822514-3822533 | MS.gene67105:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 3822171 | 3822952 | 3822171 | ID=MS.gene67105 |
| chr1.3 | mRNA | 3822171 | 3822952 | 3822171 | ID=MS.gene67105.t1;Parent=MS.gene67105 |
| chr1.3 | exon | 3822776 | 3822952 | 3822776 | ID=MS.gene67105.t1.exon1;Parent=MS.gene67105.t1 |
| chr1.3 | CDS | 3822776 | 3822952 | 3822776 | ID=cds.MS.gene67105.t1;Parent=MS.gene67105.t1 |
| chr1.3 | exon | 3822171 | 3822620 | 3822171 | ID=MS.gene67105.t1.exon2;Parent=MS.gene67105.t1 |
| chr1.3 | CDS | 3822171 | 3822620 | 3822171 | ID=cds.MS.gene67105.t1;Parent=MS.gene67105.t1 |
| Gene Sequence |
| Protein sequence |
