Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67831.t1 | XP_003594683.1 | 98.1 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 2.00E-209 | 738 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67831.t1 | Q9FGY4 | 68.2 | 365 | 106 | 4 | 1 | 361 | 1 | 359 | 7.5e-144 | 511.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67831.t1 | B7FKY1 | 98.1 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 1.5e-209 | 738.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene67831 | 0.841866 | 3.66E-58 | -1.69E-46 |
| MS.gene049368 | MS.gene67831 | 0.801465 | 8.89E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene67831 | -0.828406 | 9.15E-55 | -1.69E-46 |
| MS.gene049861 | MS.gene67831 | 0.80711 | 5.93E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene67831 | 0.821536 | 3.83E-53 | -1.69E-46 |
| MS.gene050414 | MS.gene67831 | 0.82028 | 7.46E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene67831 | 0.841438 | 4.75E-58 | -1.69E-46 |
| MS.gene050654 | MS.gene67831 | 0.828465 | 8.85E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene67831 | 0.808336 | 3.26E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene67831 | 0.82167 | 3.57E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene67831 | 0.813846 | 2.08E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene67831 | 0.817159 | 3.81E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene67831 | 0.808903 | 2.46E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene67831 | 0.807443 | 5.04E-50 | -1.69E-46 |
| MS.gene051365 | MS.gene67831 | 0.80372 | 3.05E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene67831 | 0.831584 | 1.54E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene67831 | 0.810661 | 1.03E-50 | -1.69E-46 |
| MS.gene052052 | MS.gene67831 | 0.813835 | 2.09E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene67831 | 0.804801 | 1.81E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene67831 | 0.824457 | 7.99E-54 | -1.69E-46 |
| MS.gene052932 | MS.gene67831 | 0.813624 | 2.33E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene67831 | 0.817584 | 3.06E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene67831 | 0.824773 | 6.73E-54 | -1.69E-46 |
| MS.gene053456 | MS.gene67831 | 0.802998 | 4.30E-49 | -1.69E-46 |
| MS.gene053564 | MS.gene67831 | 0.804957 | 1.68E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene67831 | 0.823714 | 1.19E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene67831 | 0.80448 | 2.12E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene67831 | 0.800719 | 1.26E-48 | -1.69E-46 |
| MS.gene054403 | MS.gene67831 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene67831 | 0.821138 | 4.74E-53 | -1.69E-46 |
| MS.gene05469 | MS.gene67831 | 0.812272 | 4.61E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene67831 | 0.803718 | 3.05E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene67831 | 0.811923 | 5.49E-51 | -1.69E-46 |
| MS.gene054957 | MS.gene67831 | 0.809225 | 2.10E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene67831 | 0.805088 | 1.58E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene67831 | 0.82564 | 4.20E-54 | -1.69E-46 |
| MS.gene056181 | MS.gene67831 | 0.803395 | 3.56E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene67831 | 0.809646 | 1.71E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene67831 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene67831 | 0.825419 | 4.74E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene67831 | 0.810731 | 9.96E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene67831 | 0.813583 | 2.38E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene67831 | 0.83873 | 2.42E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene67831 | -0.822057 | 2.90E-53 | -1.69E-46 |
| MS.gene060259 | MS.gene67831 | 0.807904 | 4.02E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene67831 | 0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene67831 | 0.800929 | 1.14E-48 | -1.69E-46 |
| MS.gene060933 | MS.gene67831 | 0.807665 | 4.52E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene67831 | 0.837227 | 5.88E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene67831 | 0.852832 | 3.56E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67831.t1 | MTR_2g033410 | 98.061 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 0.0 | 736 |
| MS.gene67831.t1 | MTR_4g133170 | 26.599 | 297 | 184 | 12 | 9 | 287 | 4 | 284 | 1.19e-16 | 80.9 |
| MS.gene67831.t1 | MTR_3g436150 | 28.571 | 322 | 178 | 15 | 8 | 295 | 31 | 334 | 2.39e-15 | 77.0 |
| MS.gene67831.t1 | MTR_3g464470 | 24.771 | 327 | 195 | 12 | 4 | 297 | 2 | 310 | 3.95e-15 | 76.3 |
| MS.gene67831.t1 | MTR_3g006570 | 24.026 | 308 | 217 | 7 | 2 | 300 | 14 | 313 | 5.32e-15 | 75.9 |
| MS.gene67831.t1 | MTR_7g407070 | 27.019 | 322 | 189 | 15 | 8 | 295 | 31 | 340 | 6.33e-15 | 75.9 |
| MS.gene67831.t1 | MTR_2g055970 | 24.497 | 298 | 187 | 11 | 9 | 286 | 211 | 490 | 2.18e-14 | 74.7 |
| MS.gene67831.t1 | MTR_3g030330 | 25.497 | 302 | 169 | 12 | 8 | 285 | 19 | 288 | 2.23e-14 | 73.9 |
| MS.gene67831.t1 | MTR_5g069760 | 25.464 | 377 | 213 | 17 | 6 | 338 | 2 | 354 | 2.26e-14 | 73.9 |
| MS.gene67831.t1 | MTR_7g070440 | 24.233 | 326 | 202 | 13 | 11 | 306 | 9 | 319 | 3.76e-14 | 73.2 |
| MS.gene67831.t1 | MTR_2g028110 | 26.667 | 300 | 188 | 11 | 9 | 287 | 4 | 292 | 4.78e-14 | 73.2 |
| MS.gene67831.t1 | MTR_3g023930 | 25.623 | 281 | 175 | 10 | 8 | 281 | 33 | 286 | 5.86e-14 | 72.8 |
| MS.gene67831.t1 | MTR_7g013590 | 26.453 | 344 | 221 | 13 | 12 | 335 | 39 | 370 | 1.18e-13 | 72.0 |
| MS.gene67831.t1 | MTR_1g094210 | 26.027 | 292 | 179 | 9 | 8 | 285 | 56 | 324 | 2.02e-13 | 71.2 |
| MS.gene67831.t1 | MTR_1g094210 | 26.027 | 292 | 179 | 9 | 8 | 285 | 56 | 324 | 2.58e-13 | 70.9 |
| MS.gene67831.t1 | MTR_2g021190 | 25.872 | 344 | 224 | 13 | 8 | 333 | 20 | 350 | 2.88e-13 | 70.9 |
| MS.gene67831.t1 | MTR_2g047200 | 23.747 | 379 | 205 | 15 | 8 | 330 | 19 | 369 | 4.09e-13 | 70.5 |
| MS.gene67831.t1 | MTR_3g010520 | 25.000 | 280 | 179 | 8 | 8 | 281 | 24 | 278 | 4.68e-13 | 70.1 |
| MS.gene67831.t1 | MTR_4g109280 | 27.397 | 292 | 171 | 13 | 10 | 283 | 9 | 277 | 5.15e-13 | 69.7 |
| MS.gene67831.t1 | MTR_3g030250 | 21.951 | 287 | 187 | 8 | 8 | 281 | 36 | 298 | 5.35e-13 | 69.7 |
| MS.gene67831.t1 | MTR_6g055210 | 24.631 | 406 | 241 | 17 | 10 | 359 | 23 | 419 | 7.72e-13 | 69.7 |
| MS.gene67831.t1 | MTR_2g047832 | 26.870 | 361 | 203 | 17 | 9 | 326 | 29 | 371 | 1.68e-12 | 68.6 |
| MS.gene67831.t1 | MTR_4g024890 | 24.691 | 324 | 186 | 11 | 7 | 293 | 8 | 310 | 2.54e-12 | 67.8 |
| MS.gene67831.t1 | MTR_3g085160 | 22.148 | 298 | 192 | 11 | 9 | 288 | 50 | 325 | 2.55e-12 | 67.8 |
| MS.gene67831.t1 | MTR_3g085160 | 22.148 | 298 | 192 | 11 | 9 | 288 | 119 | 394 | 3.38e-12 | 67.8 |
| MS.gene67831.t1 | MTR_3g464500 | 24.233 | 326 | 193 | 11 | 4 | 293 | 22 | 329 | 4.68e-12 | 67.0 |
| MS.gene67831.t1 | MTR_2g046920 | 26.912 | 353 | 209 | 17 | 4 | 326 | 13 | 346 | 5.09e-12 | 67.0 |
| MS.gene67831.t1 | MTR_4g134390 | 24.695 | 328 | 212 | 11 | 2 | 305 | 10 | 326 | 5.34e-12 | 67.0 |
| MS.gene67831.t1 | MTR_2g021150 | 26.689 | 296 | 183 | 11 | 10 | 285 | 15 | 296 | 5.51e-12 | 66.6 |
| MS.gene67831.t1 | MTR_2g046830 | 24.852 | 338 | 215 | 15 | 11 | 326 | 27 | 347 | 5.59e-12 | 67.0 |
| MS.gene67831.t1 | MTR_2g047892 | 23.869 | 398 | 238 | 17 | 8 | 360 | 21 | 398 | 7.22e-12 | 66.6 |
| MS.gene67831.t1 | MTR_7g060960 | 25.806 | 310 | 189 | 10 | 4 | 301 | 27 | 307 | 1.67e-11 | 65.5 |
| MS.gene67831.t1 | MTR_7g060940 | 25.269 | 372 | 183 | 17 | 3 | 321 | 16 | 345 | 1.77e-11 | 65.5 |
| MS.gene67831.t1 | MTR_5g096910 | 23.662 | 355 | 217 | 11 | 10 | 330 | 18 | 352 | 2.10e-11 | 65.1 |
| MS.gene67831.t1 | MTR_5g022090 | 22.297 | 296 | 180 | 7 | 9 | 285 | 34 | 298 | 2.10e-11 | 65.1 |
| MS.gene67831.t1 | MTR_7g017400 | 21.711 | 304 | 205 | 8 | 7 | 301 | 96 | 375 | 2.30e-11 | 65.1 |
| MS.gene67831.t1 | MTR_2g028130 | 22.678 | 366 | 246 | 12 | 9 | 341 | 5 | 366 | 2.61e-11 | 65.1 |
| MS.gene67831.t1 | MTR_2g021155 | 27.465 | 284 | 179 | 10 | 12 | 281 | 39 | 309 | 2.93e-11 | 65.5 |
| MS.gene67831.t1 | MTR_7g445890 | 23.929 | 397 | 228 | 17 | 8 | 359 | 15 | 382 | 3.46e-11 | 64.3 |
| MS.gene67831.t1 | MTR_4g116330 | 24.259 | 371 | 240 | 16 | 9 | 359 | 23 | 372 | 3.77e-11 | 64.3 |
| MS.gene67831.t1 | MTR_3g010590 | 23.183 | 289 | 179 | 9 | 8 | 281 | 38 | 298 | 4.92e-11 | 63.9 |
| MS.gene67831.t1 | MTR_3g084690 | 22.222 | 315 | 207 | 10 | 12 | 297 | 6 | 311 | 6.43e-11 | 63.5 |
| MS.gene67831.t1 | MTR_7g066850 | 23.229 | 353 | 213 | 11 | 8 | 321 | 22 | 355 | 6.48e-11 | 63.9 |
| MS.gene67831.t1 | MTR_2g031640 | 23.056 | 360 | 233 | 16 | 4 | 341 | 2 | 339 | 6.79e-11 | 63.5 |
| MS.gene67831.t1 | MTR_0064s0160 | 21.750 | 400 | 256 | 14 | 9 | 360 | 42 | 432 | 8.38e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67831.t1 | AT5G49610 | 68.219 | 365 | 106 | 4 | 1 | 361 | 1 | 359 | 0.0 | 509 |
| MS.gene67831.t1 | AT2G02030 | 25.249 | 301 | 175 | 7 | 9 | 270 | 39 | 328 | 1.13e-16 | 80.5 |
| MS.gene67831.t1 | AT1G33530 | 29.758 | 289 | 171 | 11 | 9 | 286 | 97 | 364 | 6.82e-16 | 79.0 |
| MS.gene67831.t1 | AT3G16210 | 26.984 | 252 | 144 | 12 | 10 | 251 | 6 | 227 | 6.57e-12 | 66.6 |
| MS.gene67831.t1 | AT1G12855 | 26.357 | 258 | 158 | 12 | 9 | 238 | 71 | 324 | 1.20e-11 | 66.2 |
Find 81 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACCATATGACCCAATTTA+TGG | 0.121920 | 2.4:-57168299 | MS.gene67831:CDS |
| TGATTTGTGTTGATAGTTTA+AGG | 0.134415 | 2.4:-57168802 | MS.gene67831:CDS |
| TTGATTTAAGTTGTGATATT+TGG | 0.191652 | 2.4:-57168334 | MS.gene67831:CDS |
| CTGTTTCCTGCTCCATAAAT+TGG | 0.196666 | 2.4:+57168287 | None:intergenic |
| CGGAAGCGTGGATGATTATC+TGG | 0.209263 | 2.4:-57168217 | MS.gene67831:CDS |
| CTGTTCTAGTATTCCGGATA+TGG | 0.232572 | 2.4:-57168705 | MS.gene67831:CDS |
| AATTATACAATGATGTTTCT+AGG | 0.255229 | 2.4:-57168883 | MS.gene67831:CDS |
| GCAAAACTGTGTGCAAATTA+TGG | 0.285845 | 2.4:-57168937 | MS.gene67831:CDS |
| AACACAAATCAAACTAGATT+TGG | 0.316362 | 2.4:+57168814 | None:intergenic |
| GGCAACTCATAAGCAGGTTT+TGG | 0.318987 | 2.4:-57168075 | MS.gene67831:CDS |
| TCAGAAGTTTAATGTTGTTC+TGG | 0.321567 | 2.4:-57168552 | MS.gene67831:CDS |
| ATCTGCATCACAACCTCATC+TGG | 0.340038 | 2.4:+57168995 | None:intergenic |
| GTTGTGCTGTTCTAGTATTC+CGG | 0.341330 | 2.4:-57168711 | MS.gene67831:CDS |
| GGATGGGAAGTTTATATGTT+TGG | 0.353754 | 2.4:-57168498 | MS.gene67831:CDS |
| ACAGGCCTCTCCCTACTCTT+AGG | 0.361424 | 2.4:+57168635 | None:intergenic |
| AACCTAAACTCACGCGTAAC+CGG | 0.364514 | 2.4:+57168662 | None:intergenic |
| ATCCGGTTACGCGTGAGTTT+AGG | 0.364961 | 2.4:-57168664 | MS.gene67831:CDS |
| CAATTTATGGAGCAGGAAAC+AGG | 0.386807 | 2.4:-57168286 | MS.gene67831:CDS |
| TCTGTCTGTGATTCAGATTT+CGG | 0.387877 | 2.4:-57168237 | MS.gene67831:CDS |
| ATAGCTCAACACTTCCGTTA+TGG | 0.391598 | 2.4:-57168004 | MS.gene67831:CDS |
| GCTCCATAAATTGGGTCATA+TGG | 0.396146 | 2.4:+57168296 | None:intergenic |
| GAAATCTCAACAAGAATCAT+AGG | 0.403273 | 2.4:+57168854 | None:intergenic |
| TGTTTCCTGCTCCATAAATT+GGG | 0.405077 | 2.4:+57168288 | None:intergenic |
| ACTTACCTGTTGGAACTTGA+TGG | 0.405989 | 2.4:-57168263 | MS.gene67831:CDS |
| TATATGTTTGGTGTTTGATT+CGG | 0.406217 | 2.4:-57168486 | MS.gene67831:CDS |
| ATCAGCGCAGGAGCCAAGTT+TGG | 0.412670 | 2.4:-57168049 | MS.gene67831:CDS |
| TGTTCTAGTATTCCGGATAT+GGG | 0.418304 | 2.4:-57168704 | MS.gene67831:CDS |
| GATTTGTGTTGATAGTTTAA+GGG | 0.421401 | 2.4:-57168801 | MS.gene67831:CDS |
| AAGGTTCGCGCGTCGTGTAA+TGG | 0.433866 | 2.4:-57168737 | MS.gene67831:CDS |
| ATTATGGTATAGGTTGACTT+TGG | 0.440149 | 2.4:-57168921 | MS.gene67831:CDS |
| AATACATTTCTTTCCAAACT+TGG | 0.442610 | 2.4:+57168036 | None:intergenic |
| TTGTTCTGGCTGGTAGTCAT+AGG | 0.443055 | 2.4:-57168538 | MS.gene67831:CDS |
| GTCATAGGACGTTTGGTCAT+AGG | 0.447514 | 2.4:-57168523 | MS.gene67831:CDS |
| CTGTGTGCAAATTATGGTAT+AGG | 0.447965 | 2.4:-57168931 | MS.gene67831:CDS |
| GGTTGGTTTAGCTTGTGATT+CGG | 0.452708 | 2.4:-57168579 | MS.gene67831:CDS |
| AAGTTTAATGTTGTTCTGGC+TGG | 0.464144 | 2.4:-57168548 | MS.gene67831:CDS |
| TCCCGATGGTGAAGCTACTT+TGG | 0.468012 | 2.4:-57168600 | MS.gene67831:CDS |
| TGAATGGTGCATTGTGGATA+AGG | 0.468222 | 2.4:-57168171 | MS.gene67831:CDS |
| GATGGTGAAGCTACTTTGGT+TGG | 0.470218 | 2.4:-57168596 | MS.gene67831:CDS |
| GGTTTACTATGTTTGCAATC+CGG | 0.471771 | 2.4:-57168681 | MS.gene67831:CDS |
| TATTCACTTGTCTGACTGAT+CGG | 0.474149 | 2.4:+57168107 | None:intergenic |
| GCTGGTAGTCATAGGACGTT+TGG | 0.476737 | 2.4:-57168530 | MS.gene67831:CDS |
| TAGGTTGCTTCCTAAGAGTA+GGG | 0.490597 | 2.4:-57168645 | MS.gene67831:CDS |
| ACCAAAGTAGCTTCACCATC+GGG | 0.500934 | 2.4:+57168599 | None:intergenic |
| TTTCTTGGCAACTCATAAGC+AGG | 0.501775 | 2.4:-57168081 | MS.gene67831:CDS |
| TGACTGATCGGGAAAATGCC+TGG | 0.505550 | 2.4:+57168119 | None:intergenic |
| TTGATTCGGAGTTGAATAAG+TGG | 0.507037 | 2.4:-57168472 | MS.gene67831:CDS |
| ATCATGGATAAATCAGGAGA+TGG | 0.507056 | 2.4:-57169025 | MS.gene67831:CDS |
| ATTTAAGTTGTGATATTTGG+AGG | 0.507064 | 2.4:-57168331 | MS.gene67831:CDS |
| GACAACCATCAAGTTCCAAC+AGG | 0.507357 | 2.4:+57168258 | None:intergenic |
| TTAGGTTGCTTCCTAAGAGT+AGG | 0.508617 | 2.4:-57168646 | MS.gene67831:CDS |
| TCATGGATAAATCAGGAGAT+GGG | 0.514329 | 2.4:-57169024 | MS.gene67831:CDS |
| GTTCTAGTATTCCGGATATG+GGG | 0.517089 | 2.4:-57168703 | MS.gene67831:CDS |
| TATGACCCAATTTATGGAGC+AGG | 0.517303 | 2.4:-57168293 | MS.gene67831:CDS |
| ATTCACTTGTCTGACTGATC+GGG | 0.518671 | 2.4:+57168108 | None:intergenic |
| GGAAGCGTGGATGATTATCT+GGG | 0.543098 | 2.4:-57168216 | MS.gene67831:CDS |
| TGTATCAGAGGAATGGTGCC+AGG | 0.547546 | 2.4:-57168137 | MS.gene67831:CDS |
| TCGGGATAAAACCGAGTCAC+AGG | 0.552202 | 2.4:+57168617 | None:intergenic |
| TTCTAGTATTCCGGATATGG+GGG | 0.554158 | 2.4:-57168702 | MS.gene67831:CDS |
| ACGTTTGGTCATAGGCCGGA+TGG | 0.559674 | 2.4:-57168515 | MS.gene67831:CDS |
| CGTTTGGTCATAGGCCGGAT+GGG | 0.563829 | 2.4:-57168514 | MS.gene67831:CDS |
| ATTTGTGTTGATAGTTTAAG+GGG | 0.568974 | 2.4:-57168800 | MS.gene67831:CDS |
| AAACATATAAACTTCCCATC+CGG | 0.573486 | 2.4:+57168500 | None:intergenic |
| AACCAAAGTAGCTTCACCAT+CGG | 0.576018 | 2.4:+57168598 | None:intergenic |
| TTGTGATGCAGATTCTTGCA+AGG | 0.583840 | 2.4:-57168985 | MS.gene67831:CDS |
| TGATTCAGATTTCGGAAGCG+TGG | 0.586875 | 2.4:-57168229 | MS.gene67831:CDS |
| ATGTTAATAATGCTTTGCAT+TGG | 0.589977 | 2.4:-57168388 | MS.gene67831:CDS |
| CTTCTGAAAAGCGATTTCAC+AGG | 0.596408 | 2.4:+57168959 | None:intergenic |
| TGCTTCCTAAGAGTAGGGAG+AGG | 0.597743 | 2.4:-57168640 | MS.gene67831:CDS |
| GTAGGGAGAGGCCTGTGACT+CGG | 0.598142 | 2.4:-57168628 | MS.gene67831:CDS |
| AAGATTACTTGATAGATGAA+TGG | 0.607504 | 2.4:-57168187 | MS.gene67831:CDS |
| TAGGACGTTTGGTCATAGGC+CGG | 0.610500 | 2.4:-57168519 | MS.gene67831:CDS |
| AGGAAACAGGACTTACCTGT+TGG | 0.624064 | 2.4:-57168273 | MS.gene67831:CDS |
| ATTCGGAGTTGAATAAGTGG+AGG | 0.625638 | 2.4:-57168469 | MS.gene67831:CDS |
| CATGGATAAATCAGGAGATG+GGG | 0.626281 | 2.4:-57169023 | MS.gene67831:CDS |
| GTGAGTCTGAGGTGTATCAG+AGG | 0.650428 | 2.4:-57168149 | MS.gene67831:CDS |
| TCTGAGGTGTATCAGAGGAA+TGG | 0.669764 | 2.4:-57168144 | MS.gene67831:CDS |
| GCATGCGCTGAAAACCATAA+CGG | 0.687822 | 2.4:+57167990 | None:intergenic |
| ACAATGTTCTCATGTAACTG+AGG | 0.694688 | 2.4:-57167957 | None:intergenic |
| TTGTGGATAAGGTGAGTCTG+AGG | 0.695028 | 2.4:-57168160 | MS.gene67831:CDS |
| GATAGATGAATGGTGCATTG+TGG | 0.697733 | 2.4:-57168177 | MS.gene67831:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATACAATGATGTTTCT+AGG | - | chr2.4:57168098-57168117 | MS.gene67831:CDS | 20.0% |
| !!! | TTGATTTAAGTTGTGATATT+TGG | - | chr2.4:57168647-57168666 | MS.gene67831:CDS | 20.0% |
| !!! | TTTTTTGAATGATAGAGTTA+AGG | - | chr2.4:57168225-57168244 | MS.gene67831:CDS | 20.0% |
| ! | AACACAAATCAAACTAGATT+TGG | + | chr2.4:57168170-57168189 | None:intergenic | 25.0% |
| ! | AAGATTACTTGATAGATGAA+TGG | - | chr2.4:57168794-57168813 | MS.gene67831:CDS | 25.0% |
| ! | AATACATTTCTTTCCAAACT+TGG | + | chr2.4:57168948-57168967 | None:intergenic | 25.0% |
| ! | ATGTTAATAATGCTTTGCAT+TGG | - | chr2.4:57168593-57168612 | MS.gene67831:CDS | 25.0% |
| !!! | ATTTAAGTTGTGATATTTGG+AGG | - | chr2.4:57168650-57168669 | MS.gene67831:CDS | 25.0% |
| !!! | ATTTGTGTTGATAGTTTAAG+GGG | - | chr2.4:57168181-57168200 | MS.gene67831:CDS | 25.0% |
| !!! | GATTTGTGTTGATAGTTTAA+GGG | - | chr2.4:57168180-57168199 | MS.gene67831:CDS | 25.0% |
| !!! | TATATGTTTGGTGTTTGATT+CGG | - | chr2.4:57168495-57168514 | MS.gene67831:CDS | 25.0% |
| !!! | TGATTTGTGTTGATAGTTTA+AGG | - | chr2.4:57168179-57168198 | MS.gene67831:CDS | 25.0% |
| AAACATATAAACTTCCCATC+CGG | + | chr2.4:57168484-57168503 | None:intergenic | 30.0% | |
| GAAATCTCAACAAGAATCAT+AGG | + | chr2.4:57168130-57168149 | None:intergenic | 30.0% | |
| TCAGAAGTTTAATGTTGTTC+TGG | - | chr2.4:57168429-57168448 | MS.gene67831:CDS | 30.0% | |
| TTACCATATGACCCAATTTA+TGG | - | chr2.4:57168682-57168701 | MS.gene67831:CDS | 30.0% | |
| ! | ATTATGGTATAGGTTGACTT+TGG | - | chr2.4:57168060-57168079 | MS.gene67831:CDS | 30.0% |
| ! | GACAAGTGAATATGTTTTCT+TGG | - | chr2.4:57168885-57168904 | MS.gene67831:CDS | 30.0% |
| AAGTTTAATGTTGTTCTGGC+TGG | - | chr2.4:57168433-57168452 | MS.gene67831:CDS | 35.0% | |
| CTGTGTGCAAATTATGGTAT+AGG | - | chr2.4:57168050-57168069 | MS.gene67831:CDS | 35.0% | |
| GCAAAACTGTGTGCAAATTA+TGG | - | chr2.4:57168044-57168063 | MS.gene67831:CDS | 35.0% | |
| GGATGGGAAGTTTATATGTT+TGG | - | chr2.4:57168483-57168502 | MS.gene67831:CDS | 35.0% | |
| GGTTTACTATGTTTGCAATC+CGG | - | chr2.4:57168300-57168319 | MS.gene67831:CDS | 35.0% | |
| TATTCACTTGTCTGACTGAT+CGG | + | chr2.4:57168877-57168896 | None:intergenic | 35.0% | |
| TCTGTCTGTGATTCAGATTT+CGG | - | chr2.4:57168744-57168763 | MS.gene67831:CDS | 35.0% | |
| TGTTCTAGTATTCCGGATAT+GGG | - | chr2.4:57168277-57168296 | MS.gene67831:CDS | 35.0% | |
| TGTTTCCTGCTCCATAAATT+GGG | + | chr2.4:57168696-57168715 | None:intergenic | 35.0% | |
| !! | TTGATTCGGAGTTGAATAAG+TGG | - | chr2.4:57168509-57168528 | MS.gene67831:CDS | 35.0% |
| AACCAAAGTAGCTTCACCAT+CGG | + | chr2.4:57168386-57168405 | None:intergenic | 40.0% | |
| ACATAGTAAACCCCCATATC+CGG | + | chr2.4:57168292-57168311 | None:intergenic | 40.0% | |
| ACTTACCTGTTGGAACTTGA+TGG | - | chr2.4:57168718-57168737 | MS.gene67831:CDS | 40.0% | |
| ATAGCTCAACACTTCCGTTA+TGG | - | chr2.4:57168977-57168996 | MS.gene67831:CDS | 40.0% | |
| ATTCACTTGTCTGACTGATC+GGG | + | chr2.4:57168876-57168895 | None:intergenic | 40.0% | |
| ATTCGGAGTTGAATAAGTGG+AGG | - | chr2.4:57168512-57168531 | MS.gene67831:CDS | 40.0% | |
| CAATTTATGGAGCAGGAAAC+AGG | - | chr2.4:57168695-57168714 | MS.gene67831:CDS | 40.0% | |
| CTGTTCTAGTATTCCGGATA+TGG | - | chr2.4:57168276-57168295 | MS.gene67831:CDS | 40.0% | |
| CTGTTTCCTGCTCCATAAAT+TGG | + | chr2.4:57168697-57168716 | None:intergenic | 40.0% | |
| GATAGATGAATGGTGCATTG+TGG | - | chr2.4:57168804-57168823 | MS.gene67831:CDS | 40.0% | |
| GCTCCATAAATTGGGTCATA+TGG | + | chr2.4:57168688-57168707 | None:intergenic | 40.0% | |
| GTTCTAGTATTCCGGATATG+GGG | - | chr2.4:57168278-57168297 | MS.gene67831:CDS | 40.0% | |
| TAGGTTGCTTCCTAAGAGTA+GGG | - | chr2.4:57168336-57168355 | MS.gene67831:CDS | 40.0% | |
| TATGACCCAATTTATGGAGC+AGG | - | chr2.4:57168688-57168707 | MS.gene67831:CDS | 40.0% | |
| TGAATGGTGCATTGTGGATA+AGG | - | chr2.4:57168810-57168829 | MS.gene67831:CDS | 40.0% | |
| TTCTAGTATTCCGGATATGG+GGG | - | chr2.4:57168279-57168298 | MS.gene67831:CDS | 40.0% | |
| TTGTGATGCAGATTCTTGCA+AGG | - | chr2.4:57167996-57168015 | MS.gene67831:CDS | 40.0% | |
| TTTCTTGGCAACTCATAAGC+AGG | - | chr2.4:57168900-57168919 | MS.gene67831:CDS | 40.0% | |
| ! | CTTCTGAAAAGCGATTTCAC+AGG | + | chr2.4:57168025-57168044 | None:intergenic | 40.0% |
| ! | GTTGTGCTGTTCTAGTATTC+CGG | - | chr2.4:57168270-57168289 | MS.gene67831:CDS | 40.0% |
| ! | TTAGGTTGCTTCCTAAGAGT+AGG | - | chr2.4:57168335-57168354 | MS.gene67831:CDS | 40.0% |
| !! | GGTTGGTTTAGCTTGTGATT+CGG | - | chr2.4:57168402-57168421 | MS.gene67831:CDS | 40.0% |
| AACCTAAACTCACGCGTAAC+CGG | + | chr2.4:57168322-57168341 | None:intergenic | 45.0% | |
| ACCAAAGTAGCTTCACCATC+GGG | + | chr2.4:57168385-57168404 | None:intergenic | 45.0% | |
| AGGAAACAGGACTTACCTGT+TGG | - | chr2.4:57168708-57168727 | MS.gene67831:CDS | 45.0% | |
| ATCTGCATCACAACCTCATC+TGG | + | chr2.4:57167989-57168008 | None:intergenic | 45.0% | |
| GACAACCATCAAGTTCCAAC+AGG | + | chr2.4:57168726-57168745 | None:intergenic | 45.0% | |
| GCATGCGCTGAAAACCATAA+CGG | + | chr2.4:57168994-57169013 | None:intergenic | 45.0% | |
| GTCATAGGACGTTTGGTCAT+AGG | - | chr2.4:57168458-57168477 | MS.gene67831:CDS | 45.0% | |
| TGATTCAGATTTCGGAAGCG+TGG | - | chr2.4:57168752-57168771 | MS.gene67831:CDS | 45.0% | |
| TTGTGGATAAGGTGAGTCTG+AGG | - | chr2.4:57168821-57168840 | MS.gene67831:CDS | 45.0% | |
| ! | AGATGGGGTTTTTCCAGATG+AGG | - | chr2.4:57167973-57167992 | MS.gene67831:CDS | 45.0% |
| ! | GATGGTGAAGCTACTTTGGT+TGG | - | chr2.4:57168385-57168404 | MS.gene67831:CDS | 45.0% |
| ! | GGAAGCGTGGATGATTATCT+GGG | - | chr2.4:57168765-57168784 | MS.gene67831:CDS | 45.0% |
| ! | GGCAACTCATAAGCAGGTTT+TGG | - | chr2.4:57168906-57168925 | MS.gene67831:CDS | 45.0% |
| ! | TCTGAGGTGTATCAGAGGAA+TGG | - | chr2.4:57168837-57168856 | MS.gene67831:CDS | 45.0% |
| ! | TTGTTCTGGCTGGTAGTCAT+AGG | - | chr2.4:57168443-57168462 | MS.gene67831:CDS | 45.0% |
| ATCCGGTTACGCGTGAGTTT+AGG | - | chr2.4:57168317-57168336 | MS.gene67831:CDS | 50.0% | |
| GCTGGTAGTCATAGGACGTT+TGG | - | chr2.4:57168451-57168470 | MS.gene67831:CDS | 50.0% | |
| TAGGACGTTTGGTCATAGGC+CGG | - | chr2.4:57168462-57168481 | MS.gene67831:CDS | 50.0% | |
| TCCCGATGGTGAAGCTACTT+TGG | - | chr2.4:57168381-57168400 | MS.gene67831:CDS | 50.0% | |
| TCGGGATAAAACCGAGTCAC+AGG | + | chr2.4:57168367-57168386 | None:intergenic | 50.0% | |
| TGACTGATCGGGAAAATGCC+TGG | + | chr2.4:57168865-57168884 | None:intergenic | 50.0% | |
| TGCTTCCTAAGAGTAGGGAG+AGG | - | chr2.4:57168341-57168360 | MS.gene67831:CDS | 50.0% | |
| TGTATCAGAGGAATGGTGCC+AGG | - | chr2.4:57168844-57168863 | MS.gene67831:CDS | 50.0% | |
| ! | CGGAAGCGTGGATGATTATC+TGG | - | chr2.4:57168764-57168783 | MS.gene67831:CDS | 50.0% |
| ! | GTGAGTCTGAGGTGTATCAG+AGG | - | chr2.4:57168832-57168851 | MS.gene67831:CDS | 50.0% |
| !! | AGGTTTTGGTGTATCAGCGC+AGG | - | chr2.4:57168920-57168939 | MS.gene67831:CDS | 50.0% |
| !!! | GTGACTCGGTTTTATCCCGA+TGG | - | chr2.4:57168367-57168386 | MS.gene67831:CDS | 50.0% |
| AAGGTTCGCGCGTCGTGTAA+TGG | - | chr2.4:57168244-57168263 | MS.gene67831:CDS | 55.0% | |
| ACAGGCCTCTCCCTACTCTT+AGG | + | chr2.4:57168349-57168368 | None:intergenic | 55.0% | |
| ACGTTTGGTCATAGGCCGGA+TGG | - | chr2.4:57168466-57168485 | MS.gene67831:CDS | 55.0% | |
| ATCAGCGCAGGAGCCAAGTT+TGG | - | chr2.4:57168932-57168951 | MS.gene67831:CDS | 55.0% | |
| CGTTTGGTCATAGGCCGGAT+GGG | - | chr2.4:57168467-57168486 | MS.gene67831:CDS | 55.0% | |
| GTAGGGAGAGGCCTGTGACT+CGG | - | chr2.4:57168353-57168372 | MS.gene67831:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 57167959 | 57169044 | 57167959 | ID=MS.gene67831 |
| chr2.4 | mRNA | 57167959 | 57169044 | 57167959 | ID=MS.gene67831.t1;Parent=MS.gene67831 |
| chr2.4 | exon | 57167959 | 57169044 | 57167959 | ID=MS.gene67831.t1.exon1;Parent=MS.gene67831.t1 |
| chr2.4 | CDS | 57167959 | 57169044 | 57167959 | ID=cds.MS.gene67831.t1;Parent=MS.gene67831.t1 |
| Gene Sequence |
| Protein sequence |
