AlfalfaGEDB | MS.gene67887

M. sativa cultivar XinJiangDaYe / MS.gene67887

KEGG: K17098
GO: GO:0003674 GO:0005488 GO:0005509 GO:0005543 GO:0005544 GO:0008289 GO:0043167 GO:0043168 GO:0043169 GO:0046872
PFAM: PF00191.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67887.t1	XP_013463074.1	95.3	233	11	0	1	233	81	313	6.70E-121	443.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67887.t1	Q94CK4	55.2	232	104	0	1	232	81	312	2.3e-69	263.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67887.t1	A0A072V514	95.3	233	11	0	1	233	81	313	4.9e-121	443.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene92405	MS.gene67887	PPI
MS.gene035891	MS.gene67887	PPI
MS.gene047020	MS.gene67887	PPI
MS.gene026445	MS.gene67887	PPI
MS.gene67887	MS.gene040097	PPI
MS.gene056195	MS.gene67887	PPI
MS.gene57291	MS.gene67887	PPI
MS.gene76197	MS.gene67887	PPI
MS.gene45772	MS.gene67887	PPI
MS.gene85401	MS.gene67887	PPI
MS.gene061777	MS.gene67887	PPI
MS.gene047030	MS.gene67887	PPI
MS.gene27444	MS.gene67887	PPI
MS.gene030809	MS.gene67887	PPI
MS.gene065031	MS.gene67887	PPI
MS.gene040887	MS.gene67887	PPI
MS.gene67887	MS.gene067369	PPI
MS.gene67887	MS.gene043964	PPI
MS.gene89484	MS.gene67887	PPI
MS.gene016642	MS.gene67887	PPI
MS.gene87117	MS.gene67887	PPI
MS.gene28077	MS.gene67887	PPI
MS.gene93736	MS.gene67887	PPI
MS.gene029299	MS.gene67887	PPI
MS.gene010215	MS.gene67887	PPI
MS.gene74945	MS.gene67887	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67887.t1	MTR_2g031980	95.279	233	11	0	1	233	81	313	6.08e-164	455
MS.gene67887.t1	MTR_3g018780	47.619	231	121	0	1	231	81	311	4.54e-77	235
MS.gene67887.t1	MTR_8g038220	48.069	233	121	0	1	233	80	312	9.76e-76	231
MS.gene67887.t1	MTR_8g038220	48.069	233	121	0	1	233	81	313	1.15e-75	231
MS.gene67887.t1	MTR_5g063670	46.121	232	125	0	1	232	82	313	2.75e-74	228
MS.gene67887.t1	MTR_3g018790	45.494	233	127	0	1	233	27	259	1.54e-70	216
MS.gene67887.t1	MTR_3g018790	45.494	233	127	0	1	233	81	313	5.89e-70	217
MS.gene67887.t1	MTR_8g038180	46.320	231	124	0	1	231	81	311	3.27e-69	215
MS.gene67887.t1	MTR_8g038210	45.494	233	126	1	1	233	81	312	6.44e-67	209
MS.gene67887.t1	MTR_8g038210	45.494	233	126	1	1	233	81	312	7.74e-67	209
MS.gene67887.t1	MTR_0276s0050	41.631	233	136	0	1	233	82	314	6.30e-60	191
MS.gene67887.t1	MTR_8g038170	42.616	237	123	4	1	231	82	311	5.87e-52	171
MS.gene67887.t1	MTR_8g038170	42.616	237	123	4	1	231	82	311	5.87e-52	171
MS.gene67887.t1	MTR_6g071605	35.931	231	146	2	2	231	83	312	2.04e-45	154
MS.gene67887.t1	MTR_6g071615	35.498	231	147	2	2	231	83	312	3.21e-42	145
MS.gene67887.t1	MTR_8g107640	33.766	231	151	2	2	231	83	312	7.31e-42	144
MS.gene67887.t1	MTR_8g038150	34.335	233	150	1	2	231	83	315	4.75e-40	140
MS.gene67887.t1	MTR_1g033560	32.618	233	155	2	2	233	83	314	9.50e-40	139
MS.gene67887.t1	MTR_1g112520	34.199	231	150	2	2	231	83	312	1.32e-39	139
MS.gene67887.t1	MTR_6g071595	33.333	231	152	2	2	231	83	312	4.94e-37	132
MS.gene67887.t1	MTR_3g018920	46.667	90	48	0	42	131	90	179	6.62e-24	94.7
MS.gene67887.t1	MTR_6g071595	30.994	171	116	2	2	171	83	252	7.26e-23	94.0
MS.gene67887.t1	MTR_3g018925	46.316	95	51	0	139	233	14	108	3.13e-21	85.5
MS.gene67887.t1	MTR_6g028030	25.974	231	167	3	3	229	77	307	1.42e-20	88.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67887.t1	AT5G12380	55.172	232	104	0	1	232	81	312	2.28e-90	269
MS.gene67887.t1	AT5G10220	45.957	235	125	1	1	233	82	316	2.52e-69	215
MS.gene67887.t1	AT5G10230	44.635	233	129	0	1	233	82	314	3.33e-69	215
MS.gene67887.t1	AT1G35720	41.880	234	135	1	1	233	82	315	1.44e-66	208
MS.gene67887.t1	AT5G65020	39.914	233	139	1	1	232	67	299	3.42e-61	194
MS.gene67887.t1	AT5G65020	39.914	233	139	1	1	232	82	314	4.11e-61	194
MS.gene67887.t1	AT2G38760	39.076	238	139	2	2	233	83	320	2.28e-49	164
MS.gene67887.t1	AT2G38750	36.709	237	137	4	1	230	68	298	2.94e-43	148
MS.gene67887.t1	AT2G38750	36.709	237	137	4	1	230	85	315	4.38e-43	148
MS.gene67887.t1	AT1G68090	31.169	231	158	1	2	231	83	313	5.38e-40	140
MS.gene67887.t1	AT1G68090	29.762	168	117	1	2	168	83	250	3.60e-25	99.8

Find 33 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGACTTTCAATATCTTTA+AGG	0.055725	2.4:+58098797	MS.gene67887:CDS
AAGTCATCTTACCTTATTAA+TGG	0.196321	2.4:-58098835	None:intergenic
TTCAATCAATTGATGAAGTA+TGG	0.245555	2.4:-58098709	None:intergenic
TTACCTTGGCCAAGTATCTT+TGG	0.262025	2.4:-58099061	None:intergenic
TTTCAATTGTAGGGATTATC+AGG	0.320601	2.4:+58098978	MS.gene67887:intron
TAGAAATACTGAAGAGCTTC+TGG	0.326830	2.4:+58098413	MS.gene67887:CDS
GTATGGCACAACCATTAATA+AGG	0.354944	2.4:+58098824	MS.gene67887:CDS
TCAATAATCACTTTGTAATC+TGG	0.360081	2.4:-58098378	None:intergenic
AATATCTTTAAGGACTTGTA+TGG	0.373979	2.4:+58098807	MS.gene67887:CDS
GCTCCAATGTATTCATCAAT+TGG	0.396159	2.4:-58099005	None:intergenic
ATTCATTTCCCAACAATTCA+AGG	0.397289	2.4:-58099363	None:intergenic
TTCATTTGCCAGATCATTCA+AGG	0.399618	2.4:-58099200	None:intergenic
GAGAGGCAACATCTTCTTCA+AGG	0.414127	2.4:-58098473	None:intergenic
CGATGACAGCCAAAGATACT+TGG	0.427900	2.4:+58099052	MS.gene67887:CDS
ATCTCACGCCTTGAATGATC+TGG	0.465214	2.4:+58099192	MS.gene67887:CDS
TCGTGCAGAGAAGGACTTGA+AGG	0.481757	2.4:+58099252	MS.gene67887:CDS
AGTGTAGCATCTCTTTCAGC+TGG	0.500813	2.4:-58098327	None:intergenic
TATTAATGAGGCACTAAAGA+AGG	0.504365	2.4:+58098350	MS.gene67887:CDS
GGCAATGCTTGTAAAGAGAA+TGG	0.512438	2.4:-58098452	None:intergenic
CCATTCATGATTCTGTTGCT+AGG	0.535196	2.4:+58099311	MS.gene67887:CDS
GATGTTGCCTCTCAAACTAC+TGG	0.536143	2.4:+58098483	MS.gene67887:CDS
ATTCTGTTGCTAGGAAGACA+TGG	0.545887	2.4:+58099320	MS.gene67887:CDS
CCTAGCAACAGAATCATGAA+TGG	0.562607	2.4:-58099311	None:intergenic
AGTCCAATTGATGAATACAT+TGG	0.565821	2.4:+58099002	MS.gene67887:CDS
TATTGTCACTCGTGCAGAGA+AGG	0.570093	2.4:+58099243	MS.gene67887:CDS
AGATGCTACACTTATTAATG+AGG	0.597858	2.4:+58098338	MS.gene67887:CDS
GCTGTCATCGATGCATCGAA+CGG	0.601285	2.4:-58099039	None:intergenic
TTCTGTTGCTAGGAAGACAT+GGG	0.615900	2.4:+58099321	MS.gene67887:CDS
AATATCACCAGTAGTTTGAG+AGG	0.620279	2.4:-58098490	None:intergenic
CAGCCAAAGATACTTGGCCA+AGG	0.646550	2.4:+58099058	MS.gene67887:CDS
AGATGAGTTTGATGAGAATG+TGG	0.686942	2.4:+58098671	MS.gene67887:CDS
ATAAGCACTTACAAGTACGA+TGG	0.694159	2.4:+58098648	MS.gene67887:CDS
TCTGTTGCTAGGAAGACATG+GGG	0.714554	2.4:+58099322	MS.gene67887:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATTATCAAATAGAGAA+TGG	-	chr2.4:58099113-58099132	None:intergenic	15.0%
!!	TATGTTTAGTAAAAACTATA+GGG	-	chr2.4:58099139-58099158	None:intergenic	15.0%
!!	GTATGTTTAGTAAAAACTAT+AGG	-	chr2.4:58099140-58099159	None:intergenic	20.0%
!!	TATTTCATGCAATAAAATCA+AGG	+	chr2.4:58098930-58098949	MS.gene67887:intron	20.0%
!!!	AATTTGTTGTTTTCAATTGT+AGG	+	chr2.4:58098968-58098987	MS.gene67887:intron	20.0%
!!!	ATTTGTTGTTTTCAATTGTA+GGG	+	chr2.4:58098969-58098988	MS.gene67887:intron	20.0%
!!!	ATTTTTATCCTTGAATTGTT+GGG	+	chr2.4:58099355-58099374	MS.gene67887:CDS	20.0%
!!!	TATGTATGTTAACTTGATTT+AGG	+	chr2.4:58099165-58099184	MS.gene67887:intron	20.0%
!	AACCAAAGTCACAAAAATAT+TGG	+	chr2.4:58099082-58099101	MS.gene67887:intron	25.0%
!	AAGTCATCTTACCTTATTAA+TGG	-	chr2.4:58098838-58098857	None:intergenic	25.0%
!	CAATAAAATCAAGGATATCA+TGG	+	chr2.4:58098939-58098958	MS.gene67887:intron	25.0%
!	TCAATAATCACTTTGTAATC+TGG	-	chr2.4:58098381-58098400	None:intergenic	25.0%
!	TGTGACTTTCAATATCTTTA+AGG	+	chr2.4:58098797-58098816	MS.gene67887:CDS	25.0%
!	TTCAATCAATTGATGAAGTA+TGG	-	chr2.4:58098712-58098731	None:intergenic	25.0%
!!	AATATCTTTAAGGACTTGTA+TGG	+	chr2.4:58098807-58098826	MS.gene67887:CDS	25.0%
!!	CATTGTTTCTCTTCAAAAAA+TGG	-	chr2.4:58099288-58099307	None:intergenic	25.0%
!!	GATTTTTATCCTTGAATTGT+TGG	+	chr2.4:58099354-58099373	MS.gene67887:CDS	25.0%
!!!	AACAGTTTTGTTATGTTACA+TGG	+	chr2.4:58098558-58098577	MS.gene67887:intron	25.0%
!!!	TTCTTTGAGAAATCTTTTGA+AGG	-	chr2.4:58098895-58098914	None:intergenic	25.0%
	AGATGCTACACTTATTAATG+AGG	+	chr2.4:58098338-58098357	MS.gene67887:CDS	30.0%
	AGTCCAATTGATGAATACAT+TGG	+	chr2.4:58099002-58099021	MS.gene67887:CDS	30.0%
	ATTCATTTCCCAACAATTCA+AGG	-	chr2.4:58099366-58099385	None:intergenic	30.0%
	TTTCAATTGTAGGGATTATC+AGG	+	chr2.4:58098978-58098997	MS.gene67887:intron	30.0%
	TTTGAACAAGTGCAACAAAA+TGG	+	chr2.4:58098588-58098607	MS.gene67887:intron	30.0%
!	AGCTTTTAACGATGATGAAA+TGG	+	chr2.4:58098737-58098756	MS.gene67887:CDS	30.0%
!	TATTAATGAGGCACTAAAGA+AGG	+	chr2.4:58098350-58098369	MS.gene67887:CDS	30.0%
!!	ACTATTTTTCATGCAGTTGT+TGG	+	chr2.4:58098617-58098636	MS.gene67887:intron	30.0%
!!	CTCCAATATTTTTGTGACTT+TGG	-	chr2.4:58099087-58099106	None:intergenic	30.0%
	AATATCACCAGTAGTTTGAG+AGG	-	chr2.4:58098493-58098512	None:intergenic	35.0%
	AGATGAGTTTGATGAGAATG+TGG	+	chr2.4:58098671-58098690	MS.gene67887:CDS	35.0%
	ATAAGCACTTACAAGTACGA+TGG	+	chr2.4:58098648-58098667	MS.gene67887:CDS	35.0%
	GCTCCAATGTATTCATCAAT+TGG	-	chr2.4:58099008-58099027	None:intergenic	35.0%
	GTATGGCACAACCATTAATA+AGG	+	chr2.4:58098824-58098843	MS.gene67887:CDS	35.0%
	TAGAAATACTGAAGAGCTTC+TGG	+	chr2.4:58098413-58098432	MS.gene67887:CDS	35.0%
	TTCATTTGCCAGATCATTCA+AGG	-	chr2.4:58099203-58099222	None:intergenic	35.0%
!	TTTTGTGACTTTGGTTACCT+TGG	-	chr2.4:58099078-58099097	None:intergenic	35.0%
	ATTCTGTTGCTAGGAAGACA+TGG	+	chr2.4:58099320-58099339	MS.gene67887:CDS	40.0%
	CCATTCATGATTCTGTTGCT+AGG	+	chr2.4:58099311-58099330	MS.gene67887:CDS	40.0%
	CCTAGCAACAGAATCATGAA+TGG	-	chr2.4:58099314-58099333	None:intergenic	40.0%
	GGCAATGCTTGTAAAGAGAA+TGG	-	chr2.4:58098455-58098474	None:intergenic	40.0%
	TTACCTTGGCCAAGTATCTT+TGG	-	chr2.4:58099064-58099083	None:intergenic	40.0%
	TTCTGTTGCTAGGAAGACAT+GGG	+	chr2.4:58099321-58099340	MS.gene67887:CDS	40.0%
	AGTGTAGCATCTCTTTCAGC+TGG	-	chr2.4:58098330-58098349	None:intergenic	45.0%
	ATCTCACGCCTTGAATGATC+TGG	+	chr2.4:58099192-58099211	MS.gene67887:CDS	45.0%
	CGATGACAGCCAAAGATACT+TGG	+	chr2.4:58099052-58099071	MS.gene67887:CDS	45.0%
	GAGAGGCAACATCTTCTTCA+AGG	-	chr2.4:58098476-58098495	None:intergenic	45.0%
	GATGTTGCCTCTCAAACTAC+TGG	+	chr2.4:58098483-58098502	MS.gene67887:CDS	45.0%
	TATTGTCACTCGTGCAGAGA+AGG	+	chr2.4:58099243-58099262	MS.gene67887:CDS	45.0%
	TCTGTTGCTAGGAAGACATG+GGG	+	chr2.4:58099322-58099341	MS.gene67887:CDS	45.0%
	CAGCCAAAGATACTTGGCCA+AGG	+	chr2.4:58099058-58099077	MS.gene67887:CDS	50.0%
	GCTGTCATCGATGCATCGAA+CGG	-	chr2.4:58099042-58099061	None:intergenic	50.0%
	TCGTGCAGAGAAGGACTTGA+AGG	+	chr2.4:58099252-58099271	MS.gene67887:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	58098321	58099387	58098321	ID=MS.gene67887
chr2.4	mRNA	58098321	58099387	58098321	ID=MS.gene67887.t1;Parent=MS.gene67887
chr2.4	exon	58098321	58098518	58098321	ID=MS.gene67887.t1.exon1;Parent=MS.gene67887.t1
chr2.4	CDS	58098321	58098518	58098321	ID=cds.MS.gene67887.t1;Parent=MS.gene67887.t1
chr2.4	exon	58098633	58098845	58098633	ID=MS.gene67887.t1.exon2;Parent=MS.gene67887.t1
chr2.4	CDS	58098633	58098845	58098633	ID=cds.MS.gene67887.t1;Parent=MS.gene67887.t1
chr2.4	exon	58098990	58099079	58098990	ID=MS.gene67887.t1.exon3;Parent=MS.gene67887.t1
chr2.4	CDS	58098990	58099079	58098990	ID=cds.MS.gene67887.t1;Parent=MS.gene67887.t1
chr2.4	exon	58099187	58099387	58099187	ID=MS.gene67887.t1.exon4;Parent=MS.gene67887.t1
chr2.4	CDS	58099187	58099387	58099187	ID=cds.MS.gene67887.t1;Parent=MS.gene67887.t1

Gene Sequence

>MS.gene67887

ATGGATCCAGCTGAAAGAGATGCTACACTTATTAATGAGGCACTAAAGAAGGCAACACCAGATTACAAAGTGATTATTGAGATTGTTTGCACTAGAAATACTGAAGAGCTTCTGGCTTTAAAGCGTTCATACCATTCTCTTTACAAGCATTGCCTTGAAGAAGATGTTGCCTCTCAAACTACTGGTGATATTCGCAAAGTATGCGACTCTCAAGCATAATTAGATTTTTATAACGAGAACAGTTTTGTTATGTTACATGGACAATGTTTTGAACAAGTGCAACAAAATGGAATGTAACTATTTTTCATGCAGTTGTTGGTTGCAGTTATAAGCACTTACAAGTACGATGGAGATGAGTTTGATGAGAATGTGGCTCATTCTGAAGCAACCATACTTCATCAATTGATTGAAAAGAAAGCTTTTAACGATGATGAAATGGTTAGAATTTTAAGCACAAGAAGTAAGAAGCAGCTATGTGTGACTTTCAATATCTTTAAGGACTTGTATGGCACAACCATTAATAAGGTAAGATGACTTTCAATACACACACATCACAGTCTTTCAGAAGTTTCCTTCAAAAGATTTCTCAAAGAAATGATATTACGAATTTATTTCATGCAATAAAATCAAGGATATCATGGAATCAAAATTTGTTGTTTTCAATTGTAGGGATTATCAGGTAGTCCAATTGATGAATACATTGGAGCACTGCGTACTACCGTTCGATGCATCGATGACAGCCAAAGATACTTGGCCAAGGTAACCAAAGTCACAAAAATATTGGAGTAACCATTCTCTATTTGATAATTTTTTAACCCTATAGTTTTTACTAAACATACGTATATATGTATGTTAACTTGATTTAGGTGTTATCTCACGCCTTGAATGATCTGGCAAATGAAGAAAATGCATTGAGTCGTGTTATTGTCACTCGTGCAGAGAAGGACTTGAAGGAAATCAATGACCATTTTTTGAAGAGAAACAATGTCACCATTCATGATTCTGTTGCTAGGAAGACATGGGGAAATTACAAGATTTTTATCCTTGAATTGTTGGGAAATGAATAA

Protein sequence

>MS.gene67887.t1

MDPAERDATLINEALKKATPDYKVIIEIVCTRNTEELLALKRSYHSLYKHCLEEDVASQTTGDIRKLLVAVISTYKYDGDEFDENVAHSEATILHQLIEKKAFNDDEMVRILSTRSKKQLCVTFNIFKDLYGTTINKGLSGSPIDEYIGALRTTVRCIDDSQRYLAKVLSHALNDLANEENALSRVIVTRAEKDLKEINDHFLKRNNVTIHDSVARKTWGNYKIFILELLGNE