AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71953


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71953.t1 MTR_4g100860 92.547 322 17 5 1 322 1 315 0.0 575
MS.gene71953.t1 MTR_0160s0030 40.872 367 160 12 1 322 1 355 2.17e-66 213
MS.gene71953.t1 MTR_5g012630 42.901 324 103 14 1 307 1 259 4.95e-59 190
MS.gene71953.t1 MTR_4g027440 33.062 369 164 9 1 310 2 346 1.53e-41 148
MS.gene71953.t1 MTR_4g082980 50.000 62 27 1 251 312 393 450 3.29e-11 64.3
MS.gene71953.t1 MTR_1g106730 31.548 168 75 6 160 312 57 199 6.49e-11 62.0
MS.gene71953.t1 MTR_4g109340 50.000 64 26 2 252 315 436 493 6.98e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71953.t1 AT3G60580 35.905 337 147 12 1 319 1 286 2.21e-47 161
MS.gene71953.t1 AT1G02030 35.821 335 127 11 2 319 3 266 2.74e-41 145
MS.gene71953.t1 AT2G45120 35.072 345 167 11 1 321 1 312 1.43e-40 144
MS.gene71953.t1 AT4G16610 40.462 173 80 5 3 173 4 155 1.76e-24 99.0
MS.gene71953.t1 AT1G26610 48.000 75 34 2 238 312 386 455 3.20e-12 67.4
MS.gene71953.t1 AT5G61470 43.902 82 36 3 205 276 218 299 3.30e-11 63.5

Find 48 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTGTTGTAGAAAATTC+TGG 0.230051 8.3:+20239742 None:intergenic
CCAAACACCTTAAAACAAAA+TGG 0.311153 8.3:+20239351 None:intergenic
TCACGTACAAAAGAAAATTC+AGG 0.344635 8.3:+20239879 None:intergenic
TCTTTAAATCTAGCAGCAAT+AGG 0.373006 8.3:+20239237 None:intergenic
CACATAGCAACATCTTCTTC+AGG 0.374474 8.3:+20239636 None:intergenic
GGCACCATGAAACTCGGCTT+TGG 0.378686 8.3:+20239717 None:intergenic
GAAACCAAAGCCGAGTTTCA+TGG 0.392923 8.3:-20239721 MS.gene71953:CDS
AAGCTTTGTTCAAGGACGTT+TGG 0.415966 8.3:-20240092 MS.gene71953:CDS
TCCGATGATGAGAAAGAATC+AGG 0.418255 8.3:+20239993 None:intergenic
TAGATCGTTGTTGACGAGTC+GGG 0.431866 8.3:+20239802 None:intergenic
TGATGCTTTCGCGAGACAAA+TGG 0.443203 8.3:-20239608 MS.gene71953:CDS
ACCTGATTCTTTCTCATCAT+CGG 0.450240 8.3:-20239994 MS.gene71953:CDS
GTGAATTGTGATGGAGTTGT+TGG 0.452696 8.3:+20239696 None:intergenic
GTTTCGATCTTCAAGAGCAT+TGG 0.459910 8.3:-20239454 MS.gene71953:CDS
TTTCGATCTTCAAGAGCATT+GGG 0.469548 8.3:-20239453 MS.gene71953:CDS
TTTGGTTCTGGTCAAGCACT+TGG 0.469573 8.3:-20239333 MS.gene71953:CDS
TTGTTGTCGTCGTTGTTGTT+TGG 0.479895 8.3:+20239765 None:intergenic
CTCGTCAACAACGATCTAAA+CGG 0.483916 8.3:-20239797 MS.gene71953:CDS
GGTTCTGGTCAAGCACTTGG+TGG 0.484093 8.3:-20239330 MS.gene71953:CDS
TGAAGAAGAAGAGGGATCGG+TGG 0.488736 8.3:-20239547 MS.gene71953:CDS
TCATCTTCTTCAAGTGGAGC+TGG 0.500458 8.3:+20239192 None:intergenic
ACAAATGCAAGCTTTGTTCA+AGG 0.502681 8.3:-20240100 MS.gene71953:CDS
GCGAAGAATGGTAATGGTAA+TGG 0.505680 8.3:-20239399 MS.gene71953:CDS
ACATAGCAACATCTTCTTCA+GGG 0.525812 8.3:+20239637 None:intergenic
AAGTTATTGAAACGAGTTCG+TGG 0.531632 8.3:-20239507 MS.gene71953:CDS
GCAGTGGAAGTTGATGAAGA+AGG 0.533864 8.3:+20239282 None:intergenic
TGTCGCGATGAAGCGAAGAA+TGG 0.548591 8.3:-20239411 MS.gene71953:CDS
TCTTGTTCAGTGAATTGTGA+TGG 0.549593 8.3:+20239687 None:intergenic
CAGTGGAAGTTGATGAAGAA+GGG 0.550853 8.3:+20239283 None:intergenic
TTTGGCAATGGAAGAGCACT+TGG 0.550999 8.3:-20240074 MS.gene71953:CDS
GTTGTTGGCACCATGAAACT+CGG 0.554243 8.3:+20239711 None:intergenic
TTAGATCGTTGTTGACGAGT+CGG 0.558622 8.3:+20239801 None:intergenic
GGAACAAGATGAAGAAGAAG+AGG 0.558902 8.3:-20239556 MS.gene71953:CDS
AGATCATCATCTTCTTCAAG+TGG 0.559232 8.3:+20239186 None:intergenic
GGCAATGGAAGAGCACTTGG+TGG 0.563472 8.3:-20240071 MS.gene71953:CDS
TGTTCAAGGACGTTTGGCAA+TGG 0.572618 8.3:-20240086 MS.gene71953:CDS
GATGAAGCGAAGAATGGTAA+TGG 0.574456 8.3:-20239405 MS.gene71953:CDS
AGCACTTGGTGGACACATGA+AGG 0.581864 8.3:-20240060 MS.gene71953:CDS
ATTAACATTAACATTAGCAG+TGG 0.584695 8.3:+20239266 None:intergenic
AGATGAAGAAGAAGAGGGAT+CGG 0.594748 8.3:-20239550 MS.gene71953:CDS
AGTTATTGAAACGAGTTCGT+GGG 0.596995 8.3:-20239506 MS.gene71953:CDS
GTATCAGAAACTGAACTCAC+AGG 0.612015 8.3:+20239663 None:intergenic
CATAGCAACATCTTCTTCAG+GGG 0.618873 8.3:+20239638 None:intergenic
GAACAAGATGAAGAAGAAGA+GGG 0.633530 8.3:-20239555 MS.gene71953:CDS
AGCATTTGTGTGAAAACTGT+GGG 0.639279 8.3:-20239482 MS.gene71953:CDS
ATCGGTGGAGAAAATATCGA+AGG 0.639870 8.3:-20239532 MS.gene71953:CDS
GATGAACAACATCAACAATG+TGG 0.658258 8.3:-20239577 MS.gene71953:CDS
AAGCATTTGTGTGAAAACTG+TGG 0.714401 8.3:-20239483 MS.gene71953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAACATTAACATTAGCAG+TGG + chr8.3:20240011-20240030 None:intergenic 25.0%
!!! TGAATGTCCATTTTGTTTTA+AGG - chr8.3:20239916-20239935 MS.gene71953:CDS 25.0%
CCAAACACCTTAAAACAAAA+TGG + chr8.3:20239926-20239945 None:intergenic 30.0%
TCACGTACAAAAGAAAATTC+AGG + chr8.3:20239398-20239417 None:intergenic 30.0%
TCTTCTGTTGTAGAAAATTC+TGG + chr8.3:20239535-20239554 None:intergenic 30.0%
TCTTTAAATCTAGCAGCAAT+AGG + chr8.3:20240040-20240059 None:intergenic 30.0%
!!! CCATTTTGTTTTAAGGTGTT+TGG - chr8.3:20239923-20239942 MS.gene71953:CDS 30.0%
AAGCATTTGTGTGAAAACTG+TGG - chr8.3:20239791-20239810 MS.gene71953:CDS 35.0%
AAGTTATTGAAACGAGTTCG+TGG - chr8.3:20239767-20239786 MS.gene71953:CDS 35.0%
ACATAGCAACATCTTCTTCA+GGG + chr8.3:20239640-20239659 None:intergenic 35.0%
ACCTGATTCTTTCTCATCAT+CGG - chr8.3:20239280-20239299 MS.gene71953:CDS 35.0%
AGATCATCATCTTCTTCAAG+TGG + chr8.3:20240091-20240110 None:intergenic 35.0%
AGCATTTGTGTGAAAACTGT+GGG - chr8.3:20239792-20239811 MS.gene71953:CDS 35.0%
AGTTATTGAAACGAGTTCGT+GGG - chr8.3:20239768-20239787 MS.gene71953:CDS 35.0%
GAACAAGATGAAGAAGAAGA+GGG - chr8.3:20239719-20239738 MS.gene71953:CDS 35.0%
GATGAACAACATCAACAATG+TGG - chr8.3:20239697-20239716 MS.gene71953:CDS 35.0%
TCTTGTTCAGTGAATTGTGA+TGG + chr8.3:20239590-20239609 None:intergenic 35.0%
!! ACAAATGCAAGCTTTGTTCA+AGG - chr8.3:20239174-20239193 MS.gene71953:CDS 35.0%
!! TTTCGATCTTCAAGAGCATT+GGG - chr8.3:20239821-20239840 MS.gene71953:CDS 35.0%
!!! GCAACTTTGAGACTTTTCTT+GGG + chr8.3:20239371-20239390 None:intergenic 35.0%
!!! TGCAACTTTGAGACTTTTCT+TGG + chr8.3:20239372-20239391 None:intergenic 35.0%
!!! TGTTTTAAGGTGTTTGGTTC+TGG - chr8.3:20239929-20239948 MS.gene71953:CDS 35.0%
AGATGAAGAAGAAGAGGGAT+CGG - chr8.3:20239724-20239743 MS.gene71953:CDS 40.0%
ATCGGTGGAGAAAATATCGA+AGG - chr8.3:20239742-20239761 MS.gene71953:CDS 40.0%
CACATAGCAACATCTTCTTC+AGG + chr8.3:20239641-20239660 None:intergenic 40.0%
CATAGCAACATCTTCTTCAG+GGG + chr8.3:20239639-20239658 None:intergenic 40.0%
CTCGTCAACAACGATCTAAA+CGG - chr8.3:20239477-20239496 MS.gene71953:CDS 40.0%
GATGAAGCGAAGAATGGTAA+TGG - chr8.3:20239869-20239888 MS.gene71953:CDS 40.0%
GGAACAAGATGAAGAAGAAG+AGG - chr8.3:20239718-20239737 MS.gene71953:CDS 40.0%
GTATCAGAAACTGAACTCAC+AGG + chr8.3:20239614-20239633 None:intergenic 40.0%
TCCGATGATGAGAAAGAATC+AGG + chr8.3:20239284-20239303 None:intergenic 40.0%
TTAGATCGTTGTTGACGAGT+CGG + chr8.3:20239476-20239495 None:intergenic 40.0%
! GTGAATTGTGATGGAGTTGT+TGG + chr8.3:20239581-20239600 None:intergenic 40.0%
! GTTTCGATCTTCAAGAGCAT+TGG - chr8.3:20239820-20239839 MS.gene71953:CDS 40.0%
! TTGTTGTCGTCGTTGTTGTT+TGG + chr8.3:20239512-20239531 None:intergenic 40.0%
!! AAGCTTTGTTCAAGGACGTT+TGG - chr8.3:20239182-20239201 MS.gene71953:CDS 40.0%
!! CAGTGGAAGTTGATGAAGAA+GGG + chr8.3:20239994-20240013 None:intergenic 40.0%
!! GCGAAGAATGGTAATGGTAA+TGG - chr8.3:20239875-20239894 MS.gene71953:CDS 40.0%
GAAACCAAAGCCGAGTTTCA+TGG - chr8.3:20239553-20239572 MS.gene71953:CDS 45.0%
GTTGTTGGCACCATGAAACT+CGG + chr8.3:20239566-20239585 None:intergenic 45.0%
TAGATCGTTGTTGACGAGTC+GGG + chr8.3:20239475-20239494 None:intergenic 45.0%
TCATCTTCTTCAAGTGGAGC+TGG + chr8.3:20240085-20240104 None:intergenic 45.0%
TGATGCTTTCGCGAGACAAA+TGG - chr8.3:20239666-20239685 MS.gene71953:CDS 45.0%
TGTTCAAGGACGTTTGGCAA+TGG - chr8.3:20239188-20239207 MS.gene71953:CDS 45.0%
!! GCAGTGGAAGTTGATGAAGA+AGG + chr8.3:20239995-20240014 None:intergenic 45.0%
!! TTTGGCAATGGAAGAGCACT+TGG - chr8.3:20239200-20239219 MS.gene71953:CDS 45.0%
!! TTTGGTTCTGGTCAAGCACT+TGG - chr8.3:20239941-20239960 MS.gene71953:CDS 45.0%
TGAAGAAGAAGAGGGATCGG+TGG - chr8.3:20239727-20239746 MS.gene71953:CDS 50.0%
TGTCGCGATGAAGCGAAGAA+TGG - chr8.3:20239863-20239882 MS.gene71953:CDS 50.0%
! AGCACTTGGTGGACACATGA+AGG - chr8.3:20239214-20239233 MS.gene71953:CDS 50.0%
GGCACCATGAAACTCGGCTT+TGG + chr8.3:20239560-20239579 None:intergenic 55.0%
! GGTTCTGGTCAAGCACTTGG+TGG - chr8.3:20239944-20239963 MS.gene71953:CDS 55.0%
!! GGCAATGGAAGAGCACTTGG+TGG - chr8.3:20239203-20239222 MS.gene71953:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 20239164 20240132 20239164 ID=MS.gene71953
chr8.3 mRNA 20239164 20240132 20239164 ID=MS.gene71953.t1;Parent=MS.gene71953
chr8.3 exon 20239164 20240132 20239164 ID=MS.gene71953.t1.exon1;Parent=MS.gene71953.t1
chr8.3 CDS 20239164 20240132 20239164 ID=cds.MS.gene71953.t1;Parent=MS.gene71953.t1
Gene Sequence

>MS.gene71953

ATGGAAAGACACAAATGCAAGCTTTGTTCAAGGACGTTTGGCAATGGAAGAGCACTTGGTGGACACATGAAGGCTCATCTTGCTATTGCTAAGTCTCAACAGAAACAATTCACTCAACCTGATTCTTTCTCATCATCGGAATCAGAACAAGAAGAAGAAGAAGAAGAAGAAAAAACTTTGATTAATTATGTTTTGAGAGAGAATCCCAAGAAAAGTCTCAAAGTTGCAGATCCTGAATTTTCTTTTGTACGTGAAACTGAGTCTGTTATTGTTCAAGATAGAGAAAGTGAGACTGAGTCAAAGAACAACCCGACTCGTCAACAACGATCTAAACGGAACAGAAAACCAAACAACAACGACGACAACAACCAGAATTTTCTACAACAGAAGAAACCAAAGCCGAGTTTCATGGTGCCAACAACTCCATCACAATTCACTGAACAAGAACCTGTGAGTTCAGTTTCTGATACTTCCCCTGAAGAAGATGTTGCTATGTGTCTCATGATGCTTTCGCGAGACAAATGGAGTAGAAAGATGAACAACATCAACAATGTGGAACAAGATGAAGAAGAAGAGGGATCGGTGGAGAAAATATCGAAGGTGAAGTTATTGAAACGAGTTCGTGGGAAGCATTTGTGTGAAAACTGTGGGAAGACGTTTCGATCTTCAAGAGCATTGGGAAGTCATAGAAGTATTTGTTGTCGCGATGAAGCGAAGAATGGTAATGGTAATGGTAAAGATGATAAGATTTTTGAATGTCCATTTTGTTTTAAGGTGTTTGGTTCTGGTCAAGCACTTGGTGGTCACAAGAGATCTCATCTCATGATCCCTTCTTCATCAACTTCCACTGCTAATGTTAATGTTAATGTTAATCCTATTGCTGCTAGATTTAAAGAGAGTTTCATAGATCTCAATATGCCAGCTCCACTTGAAGAAGATGATGATCTTAGTGTTGTTTCTGATGCCTAA

Protein sequence

>MS.gene71953.t1

MERHKCKLCSRTFGNGRALGGHMKAHLAIAKSQQKQFTQPDSFSSSESEQEEEEEEEKTLINYVLRENPKKSLKVADPEFSFVRETESVIVQDRESETESKNNPTRQQRSKRNRKPNNNDDNNQNFLQQKKPKPSFMVPTTPSQFTEQEPVSSVSDTSPEEDVAMCLMMLSRDKWSRKMNNINNVEQDEEEEGSVEKISKVKLLKRVRGKHLCENCGKTFRSSRALGSHRSICCRDEAKNGNGNGKDDKIFECPFCFKVFGSGQALGGHKRSHLMIPSSSTSTANVNVNVNPIAARFKESFIDLNMPAPLEEDDDLSVVSDA