Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73564.t1 | GAV77549.1 | 67.9 | 53 | 14 | 1 | 5 | 54 | 217 | 269 | 5.30E-10 | 73.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73564.t1 | A2XK30 | 60.4 | 53 | 18 | 1 | 5 | 54 | 237 | 289 | 1.2e-10 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73564.t1 | A0A1Q3CBD6 | 67.9 | 53 | 14 | 1 | 5 | 54 | 217 | 269 | 3.8e-10 | 73.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene73564 | 0.809077 | 2.26E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene73564 | 0.821351 | 4.23E-53 | -1.69E-46 |
| MS.gene049272 | MS.gene73564 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene049343 | MS.gene73564 | 0.815243 | 1.02E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene73564 | 0.805055 | 1.61E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene73564 | 0.822786 | 1.97E-53 | -1.69E-46 |
| MS.gene049873 | MS.gene73564 | 0.819978 | 8.74E-53 | -1.69E-46 |
| MS.gene050026 | MS.gene73564 | 0.814467 | 1.52E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene73564 | 0.825527 | 4.47E-54 | -1.69E-46 |
| MS.gene050287 | MS.gene73564 | -0.820205 | 7.75E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene73564 | 0.833614 | 4.82E-56 | -1.69E-46 |
| MS.gene050799 | MS.gene73564 | 0.819258 | 1.28E-52 | -1.69E-46 |
| MS.gene050844 | MS.gene73564 | 0.831299 | 1.81E-55 | -1.69E-46 |
| MS.gene051181 | MS.gene73564 | 0.820653 | 6.12E-53 | -1.69E-46 |
| MS.gene051594 | MS.gene73564 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene73564 | 0.834557 | 2.80E-56 | -1.69E-46 |
| MS.gene051957 | MS.gene73564 | 0.809761 | 1.61E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene73564 | 0.826241 | 3.02E-54 | -1.69E-46 |
| MS.gene052208 | MS.gene73564 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene73564 | 0.872144 | 3.98E-67 | -1.69E-46 |
| MS.gene052584 | MS.gene73564 | 0.804905 | 1.73E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene73564 | 0.818738 | 1.68E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene73564 | 0.822657 | 2.11E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene73564 | 0.832254 | 1.05E-55 | -1.69E-46 |
| MS.gene053145 | MS.gene73564 | 0.854516 | 1.17E-61 | -1.69E-46 |
| MS.gene053146 | MS.gene73564 | 0.810438 | 1.15E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene73564 | 0.821411 | 4.10E-53 | -1.69E-46 |
| MS.gene053900 | MS.gene73564 | 0.837199 | 5.99E-57 | -1.69E-46 |
| MS.gene053966 | MS.gene73564 | 0.807344 | 5.29E-50 | -1.69E-46 |
| MS.gene053967 | MS.gene73564 | 0.814509 | 1.49E-51 | -1.69E-46 |
| MS.gene053998 | MS.gene73564 | 0.809844 | 1.55E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene73564 | 0.801331 | 9.47E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene73564 | 0.800388 | 1.48E-48 | -1.69E-46 |
| MS.gene054582 | MS.gene73564 | 0.824113 | 9.62E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene73564 | 0.860629 | 1.81E-63 | -1.69E-46 |
| MS.gene054775 | MS.gene73564 | 0.818212 | 2.21E-52 | -1.69E-46 |
| MS.gene055374 | MS.gene73564 | 0.819437 | 1.16E-52 | -1.69E-46 |
| MS.gene055586 | MS.gene73564 | 0.817721 | 2.85E-52 | -1.69E-46 |
| MS.gene055589 | MS.gene73564 | 0.822022 | 2.96E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene73564 | 0.807951 | 3.93E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene73564 | 0.856796 | 2.52E-62 | -1.69E-46 |
| MS.gene056050 | MS.gene73564 | 0.816537 | 5.26E-52 | -1.69E-46 |
| MS.gene056162 | MS.gene73564 | 0.808681 | 2.75E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene73564 | 0.806173 | 9.35E-50 | -1.69E-46 |
| MS.gene056373 | MS.gene73564 | 0.816582 | 5.13E-52 | -1.69E-46 |
| MS.gene056424 | MS.gene73564 | 0.8093 | 2.03E-50 | -1.69E-46 |
| MS.gene056543 | MS.gene73564 | 0.803398 | 3.55E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene73564 | 0.802687 | 4.98E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene73564 | 0.803806 | 2.92E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene73564 | 0.818673 | 1.73E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene73564 | 0.806085 | 9.76E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene73564 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene73564 | 0.818861 | 1.57E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene73564 | 0.840286 | 9.53E-58 | -1.69E-46 |
| MS.gene058625 | MS.gene73564 | 0.850513 | 1.62E-60 | -1.69E-46 |
| MS.gene058783 | MS.gene73564 | 0.827185 | 1.80E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene73564 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene73564 | 0.83399 | 3.88E-56 | -1.69E-46 |
| MS.gene059272 | MS.gene73564 | 0.824674 | 7.10E-54 | -1.69E-46 |
| MS.gene05954 | MS.gene73564 | 0.817625 | 2.99E-52 | -1.69E-46 |
| MS.gene059842 | MS.gene73564 | 0.80099 | 1.11E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene73564 | 0.836437 | 9.36E-57 | -1.69E-46 |
| MS.gene060521 | MS.gene73564 | 0.816298 | 5.94E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene73564 | 0.802293 | 6.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73564.t1 | MTR_2g101190 | 67.925 | 53 | 14 | 1 | 5 | 54 | 214 | 266 | 2.55e-17 | 75.1 |
| MS.gene73564.t1 | MTR_8g013980 | 64.151 | 53 | 16 | 1 | 5 | 54 | 212 | 264 | 1.18e-15 | 70.5 |
| MS.gene73564.t1 | MTR_2g030130 | 58.491 | 53 | 19 | 1 | 5 | 54 | 217 | 269 | 1.86e-15 | 70.1 |
| MS.gene73564.t1 | MTR_3g109800 | 58.491 | 53 | 19 | 1 | 5 | 54 | 216 | 268 | 3.01e-14 | 66.6 |
| MS.gene73564.t1 | MTR_3g109800 | 58.491 | 53 | 19 | 1 | 5 | 54 | 216 | 268 | 3.91e-14 | 66.2 |
| MS.gene73564.t1 | MTR_3g109800 | 58.491 | 53 | 19 | 1 | 5 | 54 | 216 | 268 | 1.12e-13 | 64.7 |
| MS.gene73564.t1 | MTR_2g094520 | 49.057 | 53 | 24 | 1 | 5 | 54 | 220 | 272 | 7.02e-12 | 59.7 |
| MS.gene73564.t1 | MTR_4g058970 | 49.057 | 53 | 24 | 1 | 5 | 54 | 223 | 275 | 1.22e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73564.t1 | AT1G52150 | 56.604 | 53 | 20 | 1 | 5 | 54 | 217 | 269 | 1.77e-14 | 67.4 |
| MS.gene73564.t1 | AT1G52150 | 56.604 | 53 | 20 | 1 | 5 | 54 | 217 | 269 | 1.78e-14 | 67.4 |
| MS.gene73564.t1 | AT1G52150 | 56.604 | 53 | 20 | 1 | 5 | 54 | 217 | 269 | 1.78e-14 | 67.4 |
| MS.gene73564.t1 | AT2G34710 | 58.491 | 53 | 19 | 1 | 5 | 54 | 230 | 282 | 8.13e-14 | 65.5 |
| MS.gene73564.t1 | AT5G60690 | 52.830 | 53 | 22 | 1 | 5 | 54 | 217 | 269 | 1.18e-13 | 65.1 |
| MS.gene73564.t1 | AT1G30490 | 54.717 | 53 | 21 | 1 | 5 | 54 | 226 | 278 | 1.03e-12 | 62.4 |
| MS.gene73564.t1 | AT4G32880 | 54.717 | 53 | 21 | 1 | 5 | 54 | 216 | 268 | 4.21e-12 | 60.5 |
Find 24 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTCCAGCCAACAAATTT+AGG | 0.187535 | 6.4:+57816107 | MS.gene73564:intron |
| CTTGCTGATCCTAAATTTGT+TGG | 0.321349 | 6.4:-57816116 | None:intergenic |
| AGCTATCGTCTTGAGGATTA+TGG | 0.321477 | 6.4:+57816166 | MS.gene73564:CDS |
| CTGCATGTATTGCAGCTCTA+TGG | 0.327181 | 6.4:-57815853 | None:intergenic |
| ACCACGAAGTCCGATCCTTC+AGG | 0.362970 | 6.4:-57815773 | None:intergenic |
| ATGGTTCCACCATTTGTGTT+GGG | 0.424035 | 6.4:-57815834 | None:intergenic |
| TATGGTTCCACCATTTGTGT+TGG | 0.455098 | 6.4:-57815835 | None:intergenic |
| GACGATAGCTAGAGACTCAT+AGG | 0.476917 | 6.4:-57816153 | None:intergenic |
| TCCTGAAGGATCGGACTTCG+TGG | 0.492081 | 6.4:+57815772 | MS.gene73564:CDS |
| ATTGTCCTTCCCAACACAAA+TGG | 0.508396 | 6.4:+57815825 | MS.gene73564:CDS |
| TTCCACCATTTGTGTTGGGA+AGG | 0.538380 | 6.4:-57815830 | None:intergenic |
| TTCGTGGTATCGTGATAGCC+AGG | 0.540887 | 6.4:+57815788 | MS.gene73564:CDS |
| TTCTAACGTACTTGATGCGG+AGG | 0.574965 | 6.4:-57815411 | None:intergenic |
| GTATCGTGATAGCCAGGCTG+TGG | 0.577136 | 6.4:+57815794 | MS.gene73564:CDS |
| GTCCTTCCCAACACAAATGG+TGG | 0.593510 | 6.4:+57815828 | MS.gene73564:CDS |
| CATAGAGCTGCAATACATGC+AGG | 0.594541 | 6.4:+57815854 | MS.gene73564:CDS |
| TATTTCTAACGTACTTGATG+CGG | 0.597498 | 6.4:-57815414 | None:intergenic |
| AGTCTCTAGCTATCGTCTTG+AGG | 0.600364 | 6.4:+57816159 | MS.gene73564:CDS |
| TTGCAGATATCCTGAAGGAT+CGG | 0.600491 | 6.4:+57815763 | MS.gene73564:CDS |
| TTATGGAGCATCTTCACCAA+GGG | 0.620476 | 6.4:+57816183 | MS.gene73564:CDS |
| CAATCATTATATCCACAGCC+TGG | 0.623559 | 6.4:-57815806 | None:intergenic |
| ACAGGTTGCAGATATCCTGA+AGG | 0.627860 | 6.4:+57815758 | MS.gene73564:intron |
| AATTTAGGATCAGCAAGAGA+TGG | 0.634057 | 6.4:+57816122 | MS.gene73564:CDS |
| ATTATGGAGCATCTTCACCA+AGG | 0.653602 | 6.4:+57816182 | MS.gene73564:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAATATCTAGTTATCCCT+TGG | - | chr6.4:57816202-57816221 | None:intergenic | 25.0% |
| !!! | GGGATAACTAGATATTTTTA+TGG | + | chr6.4:57816203-57816222 | MS.gene73564:CDS | 25.0% |
| !!! | GTTCTAAAACAATGATTTGT+TGG | - | chr6.4:57816059-57816078 | None:intergenic | 25.0% |
| ATACTTTCTTGACTGAAAGA+TGG | - | chr6.4:57815938-57815957 | None:intergenic | 30.0% | |
| ! | TATTTCTAACGTACTTGATG+CGG | - | chr6.4:57815417-57815436 | None:intergenic | 30.0% |
| !! | TAAAACAATGATTTGTTGGC+TGG | - | chr6.4:57816055-57816074 | None:intergenic | 30.0% |
| AAACCAAGCATACTCTTTAC+AGG | + | chr6.4:57815740-57815759 | MS.gene73564:intron | 35.0% | |
| CTTGCTGATCCTAAATTTGT+TGG | - | chr6.4:57816119-57816138 | None:intergenic | 35.0% | |
| TATTGACAATACTGATGCGA+AGG | - | chr6.4:57815987-57816006 | None:intergenic | 35.0% | |
| TCTTTCCAGCCAACAAATTT+AGG | + | chr6.4:57816107-57816126 | MS.gene73564:intron | 35.0% | |
| TGCGTATATAGTCAAGTGTA+TGG | + | chr6.4:57815554-57815573 | MS.gene73564:intron | 35.0% | |
| TTATATAGTATGCCCTACTG+TGG | + | chr6.4:57815612-57815631 | MS.gene73564:intron | 35.0% | |
| ! | AATTTAGGATCAGCAAGAGA+TGG | + | chr6.4:57816122-57816141 | MS.gene73564:CDS | 35.0% |
| !! | CTGCTTTTCGAGAAGTTTAT+TGG | + | chr6.4:57815587-57815606 | MS.gene73564:intron | 35.0% |
| AGCTATCGTCTTGAGGATTA+TGG | + | chr6.4:57816166-57816185 | MS.gene73564:CDS | 40.0% | |
| ATCATGCCATCGATGAACAT+TGG | - | chr6.4:57815720-57815739 | None:intergenic | 40.0% | |
| ATGGTTCCACCATTTGTGTT+GGG | - | chr6.4:57815837-57815856 | None:intergenic | 40.0% | |
| ATTGTCCTTCCCAACACAAA+TGG | + | chr6.4:57815825-57815844 | MS.gene73564:CDS | 40.0% | |
| CAATCATTATATCCACAGCC+TGG | - | chr6.4:57815809-57815828 | None:intergenic | 40.0% | |
| TATGGTTCCACCATTTGTGT+TGG | - | chr6.4:57815838-57815857 | None:intergenic | 40.0% | |
| TTGCAGATATCCTGAAGGAT+CGG | + | chr6.4:57815763-57815782 | MS.gene73564:CDS | 40.0% | |
| TTGTAGGTTATAGACCCACT+AGG | - | chr6.4:57815654-57815673 | None:intergenic | 40.0% | |
| ! | AACAGTTCAGCAAACTTGCT+TGG | - | chr6.4:57815475-57815494 | None:intergenic | 40.0% |
| ! | ATTATGGAGCATCTTCACCA+AGG | + | chr6.4:57816182-57816201 | MS.gene73564:CDS | 40.0% |
| ! | CAACCTGTAAAGAGTATGCT+TGG | - | chr6.4:57815746-57815765 | None:intergenic | 40.0% |
| ! | CTTTTGCCAATGTTCATCGA+TGG | + | chr6.4:57815711-57815730 | MS.gene73564:intron | 40.0% |
| ! | TAGTGGGTCTATAACCTACA+AGG | + | chr6.4:57815653-57815672 | MS.gene73564:intron | 40.0% |
| ! | TTATGGAGCATCTTCACCAA+GGG | + | chr6.4:57816183-57816202 | MS.gene73564:CDS | 40.0% |
| ! | TTCAGAGCACTAATCCTTGT+AGG | - | chr6.4:57815670-57815689 | None:intergenic | 40.0% |
| !! | CTGATCCTAAATTTGTTGGC+TGG | - | chr6.4:57816115-57816134 | None:intergenic | 40.0% |
| ACAGGTTGCAGATATCCTGA+AGG | + | chr6.4:57815758-57815777 | MS.gene73564:intron | 45.0% | |
| AGGCGAGTACAAAATGACGA+TGG | - | chr6.4:57815967-57815986 | None:intergenic | 45.0% | |
| AGTCTCTAGCTATCGTCTTG+AGG | + | chr6.4:57816159-57816178 | MS.gene73564:CDS | 45.0% | |
| CATAGAGCTGCAATACATGC+AGG | + | chr6.4:57815854-57815873 | MS.gene73564:CDS | 45.0% | |
| CTGCATGTATTGCAGCTCTA+TGG | - | chr6.4:57815856-57815875 | None:intergenic | 45.0% | |
| GACGATAGCTAGAGACTCAT+AGG | - | chr6.4:57816156-57816175 | None:intergenic | 45.0% | |
| TTCCACCATTTGTGTTGGGA+AGG | - | chr6.4:57815833-57815852 | None:intergenic | 45.0% | |
| TTCTAACGTACTTGATGCGG+AGG | - | chr6.4:57815414-57815433 | None:intergenic | 45.0% | |
| GTCCTTCCCAACACAAATGG+TGG | + | chr6.4:57815828-57815847 | MS.gene73564:CDS | 50.0% | |
| TTCGTGGTATCGTGATAGCC+AGG | + | chr6.4:57815788-57815807 | MS.gene73564:CDS | 50.0% | |
| ACCACGAAGTCCGATCCTTC+AGG | - | chr6.4:57815776-57815795 | None:intergenic | 55.0% | |
| GTATCGTGATAGCCAGGCTG+TGG | + | chr6.4:57815794-57815813 | MS.gene73564:CDS | 55.0% | |
| TCCTGAAGGATCGGACTTCG+TGG | + | chr6.4:57815772-57815791 | MS.gene73564:CDS | 55.0% | |
| ! | ATCCGCTTGCTGCCACAGTA+GGG | - | chr6.4:57815627-57815646 | None:intergenic | 55.0% |
| ACTGTGGCAGCAAGCGGATG+TGG | + | chr6.4:57815628-57815647 | MS.gene73564:intron | 60.0% | |
| AGCAAGCGGATGTGGCCTAG+TGG | + | chr6.4:57815636-57815655 | MS.gene73564:intron | 60.0% | |
| GCAAGCGGATGTGGCCTAGT+GGG | + | chr6.4:57815637-57815656 | MS.gene73564:intron | 60.0% | |
| TGCCCTACTGTGGCAGCAAG+CGG | + | chr6.4:57815622-57815641 | MS.gene73564:intron | 60.0% | |
| ! | CATCCGCTTGCTGCCACAGT+AGG | - | chr6.4:57815628-57815647 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 57815402 | 57816247 | 57815402 | ID=MS.gene73564 |
| chr6.4 | mRNA | 57815402 | 57816247 | 57815402 | ID=MS.gene73564.t1;Parent=MS.gene73564 |
| chr6.4 | exon | 57815402 | 57815422 | 57815402 | ID=MS.gene73564.t1.exon1;Parent=MS.gene73564.t1 |
| chr6.4 | CDS | 57815402 | 57815422 | 57815402 | ID=cds.MS.gene73564.t1;Parent=MS.gene73564.t1 |
| chr6.4 | exon | 57815762 | 57815875 | 57815762 | ID=MS.gene73564.t1.exon2;Parent=MS.gene73564.t1 |
| chr6.4 | CDS | 57815762 | 57815875 | 57815762 | ID=cds.MS.gene73564.t1;Parent=MS.gene73564.t1 |
| chr6.4 | exon | 57816116 | 57816247 | 57816116 | ID=MS.gene73564.t1.exon3;Parent=MS.gene73564.t1 |
| chr6.4 | CDS | 57816116 | 57816247 | 57816116 | ID=cds.MS.gene73564.t1;Parent=MS.gene73564.t1 |
| Gene Sequence |
| Protein sequence |
