Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89368.t1 | XP_003621026.1 | 100 | 96 | 0 | 0 | 1 | 96 | 63 | 158 | 3.20E-45 | 190.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89368.t1 | Q67YI9 | 83.3 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 1.6e-40 | 166.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89368.t1 | A2Q4X4 | 100.0 | 96 | 0 | 0 | 1 | 96 | 63 | 158 | 2.3e-45 | 190.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene89368 | 0.824261 | 8.88E-54 | -1.69E-46 |
| MS.gene049283 | MS.gene89368 | 0.80462 | 1.98E-49 | -1.69E-46 |
| MS.gene050213 | MS.gene89368 | 0.825865 | 3.71E-54 | -1.69E-46 |
| MS.gene050224 | MS.gene89368 | 0.847056 | 1.47E-59 | -1.69E-46 |
| MS.gene050359 | MS.gene89368 | 0.837444 | 5.18E-57 | -1.69E-46 |
| MS.gene050414 | MS.gene89368 | 0.829386 | 5.30E-55 | -1.69E-46 |
| MS.gene050551 | MS.gene89368 | 0.816452 | 5.49E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene89368 | 0.830783 | 2.42E-55 | -1.69E-46 |
| MS.gene050654 | MS.gene89368 | 0.830612 | 2.66E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene89368 | 0.803009 | 4.28E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene89368 | 0.809655 | 1.70E-50 | -1.69E-46 |
| MS.gene051852 | MS.gene89368 | 0.802255 | 6.12E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene89368 | 0.827096 | 1.89E-54 | -1.69E-46 |
| MS.gene052118 | MS.gene89368 | 0.802489 | 5.48E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene89368 | 0.84264 | 2.29E-58 | -1.69E-46 |
| MS.gene052463 | MS.gene89368 | 0.816694 | 4.85E-52 | -1.69E-46 |
| MS.gene052514 | MS.gene89368 | 0.842492 | 2.50E-58 | -1.69E-46 |
| MS.gene052609 | MS.gene89368 | 0.87115 | 8.51E-67 | -1.69E-46 |
| MS.gene052775 | MS.gene89368 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene052776 | MS.gene89368 | 0.834356 | 3.14E-56 | -1.69E-46 |
| MS.gene053079 | MS.gene89368 | 0.805604 | 1.23E-49 | -1.69E-46 |
| MS.gene053143 | MS.gene89368 | 0.851262 | 9.95E-61 | -1.69E-46 |
| MS.gene053620 | MS.gene89368 | 0.819258 | 1.28E-52 | -1.69E-46 |
| MS.gene054178 | MS.gene89368 | 0.805086 | 1.58E-49 | -1.69E-46 |
| MS.gene054641 | MS.gene89368 | 0.813899 | 2.03E-51 | -1.69E-46 |
| MS.gene054824 | MS.gene89368 | 0.804793 | 1.82E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene89368 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene89368 | 0.816741 | 4.73E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene89368 | 0.812688 | 3.74E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene89368 | 0.838743 | 2.40E-57 | -1.69E-46 |
| MS.gene055864 | MS.gene89368 | 0.818291 | 2.12E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene89368 | 0.822826 | 1.92E-53 | -1.69E-46 |
| MS.gene056308 | MS.gene89368 | 0.807065 | 6.06E-50 | -1.69E-46 |
| MS.gene056507 | MS.gene89368 | 0.838849 | 2.25E-57 | -1.69E-46 |
| MS.gene056519 | MS.gene89368 | 0.822585 | 2.19E-53 | -1.69E-46 |
| MS.gene056699 | MS.gene89368 | 0.800085 | 1.70E-48 | -1.69E-46 |
| MS.gene056831 | MS.gene89368 | 0.818098 | 2.34E-52 | -1.69E-46 |
| MS.gene057034 | MS.gene89368 | 0.803493 | 3.40E-49 | -1.69E-46 |
| MS.gene057371 | MS.gene89368 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene057919 | MS.gene89368 | 0.817558 | 3.10E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene89368 | 0.824839 | 6.49E-54 | -1.69E-46 |
| MS.gene058289 | MS.gene89368 | 0.821201 | 4.58E-53 | -1.69E-46 |
| MS.gene058292 | MS.gene89368 | 0.805389 | 1.37E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene89368 | 0.803951 | 2.73E-49 | -1.69E-46 |
| MS.gene058638 | MS.gene89368 | 0.808775 | 2.62E-50 | -1.69E-46 |
| MS.gene058639 | MS.gene89368 | 0.804139 | 2.49E-49 | -1.69E-46 |
| MS.gene058647 | MS.gene89368 | 0.819408 | 1.18E-52 | -1.69E-46 |
| MS.gene058649 | MS.gene89368 | 0.815022 | 1.14E-51 | -1.69E-46 |
| MS.gene059007 | MS.gene89368 | 0.817678 | 2.91E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene89368 | 0.807573 | 4.73E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene89368 | 0.835526 | 1.59E-56 | -1.69E-46 |
| MS.gene059965 | MS.gene89368 | 0.811329 | 7.39E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene89368 | 0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene060777 | MS.gene89368 | 0.82127 | 4.42E-53 | -1.69E-46 |
| MS.gene060785 | MS.gene89368 | 0.804831 | 1.79E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene89368 | 0.828588 | 8.27E-55 | -1.69E-46 |
| MS.gene060933 | MS.gene89368 | 0.827671 | 1.38E-54 | -1.69E-46 |
| MS.gene061006 | MS.gene89368 | 0.845011 | 5.30E-59 | -1.69E-46 |
| MS.gene061100 | MS.gene89368 | 0.855538 | 5.90E-62 | -1.69E-46 |
| MS.gene061160 | MS.gene89368 | 0.80116 | 1.03E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89368.t1 | MTR_7g006280 | 100.000 | 96 | 0 | 0 | 1 | 96 | 63 | 158 | 1.36e-62 | 204 |
| MS.gene89368.t1 | MTR_7g006100 | 91.579 | 95 | 8 | 0 | 2 | 96 | 637 | 731 | 1.60e-55 | 183 |
| MS.gene89368.t1 | MTR_1g016980 | 58.333 | 96 | 40 | 0 | 1 | 96 | 65 | 160 | 2.32e-37 | 131 |
| MS.gene89368.t1 | MTR_4g063895 | 41.772 | 79 | 46 | 0 | 13 | 91 | 48 | 126 | 2.44e-13 | 63.5 |
| MS.gene89368.t1 | MTR_4g063895 | 42.308 | 78 | 45 | 0 | 13 | 90 | 83 | 160 | 1.23e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89368.t1 | AT2G43160 | 83.333 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 6.31e-54 | 177 |
| MS.gene89368.t1 | AT2G43160 | 83.333 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 2.66e-53 | 177 |
| MS.gene89368.t1 | AT2G43160 | 83.333 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 2.66e-53 | 177 |
| MS.gene89368.t1 | AT2G43160 | 83.333 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 2.66e-53 | 177 |
| MS.gene89368.t1 | AT2G43160 | 83.333 | 96 | 16 | 0 | 1 | 96 | 63 | 158 | 2.66e-53 | 177 |
| MS.gene89368.t1 | AT3G59290 | 78.125 | 96 | 21 | 0 | 1 | 96 | 63 | 158 | 4.03e-51 | 172 |
| MS.gene89368.t1 | AT5G11710 | 57.895 | 95 | 40 | 0 | 1 | 95 | 65 | 159 | 2.81e-36 | 129 |
| MS.gene89368.t1 | AT5G11710 | 57.895 | 95 | 40 | 0 | 1 | 95 | 65 | 159 | 2.81e-36 | 129 |
| MS.gene89368.t1 | AT5G11710 | 57.895 | 95 | 40 | 0 | 1 | 95 | 65 | 159 | 2.81e-36 | 129 |
| MS.gene89368.t1 | AT3G23350 | 39.241 | 79 | 48 | 0 | 13 | 91 | 86 | 164 | 1.51e-12 | 61.6 |
| MS.gene89368.t1 | AT3G23350 | 39.241 | 79 | 48 | 0 | 13 | 91 | 87 | 165 | 1.68e-12 | 61.6 |
Find 0 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GGTTACATTATAATATTTTA+CGG | - | 50654:26603-26622 | None:intergenic | 15.0% |
| !! | ACTAACAGCATATAAATATT+AGG | - | 50654:26544-26563 | None:intergenic | 20.0% |
| !! | AGAACATTCTTATCAAATAT+CGG | + | 50654:26494-26513 | MS.gene89368:CDS | 20.0% |
| !!! | AGGAATATTACATTTTTATC+AGG | - | 50654:26624-26643 | None:intergenic | 20.0% |
| ! | GGAATCAATATATTGAAAGT+CGG | - | 50654:26662-26681 | None:intergenic | 25.0% |
| ! | TTCTTTCTTTATCATTCACA+AGG | - | 50654:26738-26757 | None:intergenic | 25.0% |
| !! | ATGTCTACAAGGTATTAATT+CGG | + | 50654:26290-26309 | MS.gene89368:intron | 25.0% |
| !!! | TTTCAATATATTGATTCCAG+TGG | + | 50654:26664-26683 | MS.gene89368:CDS | 25.0% |
| AATCAGTAAATGATGCAGAA+GGG | - | 50654:26344-26363 | None:intergenic | 30.0% | |
| ATTCGGAATACTTTATGTTC+TGG | + | 50654:26307-26326 | MS.gene89368:intron | 30.0% | |
| TGATAAAGAAAGAATCGTAG+AGG | + | 50654:26744-26763 | MS.gene89368:CDS | 30.0% | |
| AAGAAAGAATCGTAGAGGTT+AGG | + | 50654:26749-26768 | MS.gene89368:CDS | 35.0% | |
| CAATCAGTAAATGATGCAGA+AGG | - | 50654:26345-26364 | None:intergenic | 35.0% | |
| TTTGACAGTACTTGAGTACT+TGG | + | 50654:26434-26453 | MS.gene89368:CDS | 35.0% | |
| ACGGTCATTTGATCGAAACT+AGG | - | 50654:26584-26603 | None:intergenic | 40.0% | |
| ACTGCTCACTGTTCATTAAC+AGG | + | 50654:26410-26429 | MS.gene89368:intron | 40.0% | |
| CTGTTAGTCAATTTCCACAC+AGG | + | 50654:26556-26575 | MS.gene89368:intron | 40.0% | |
| GAGATCAAGGAAACAATGTC+AGG | + | 50654:26689-26708 | MS.gene89368:CDS | 40.0% | |
| TGGAAACGAATTAACGACAC+TGG | + | 50654:26253-26272 | MS.gene89368:CDS | 40.0% | |
| TTAACGACACTGGCAAGAAT+TGG | + | 50654:26263-26282 | MS.gene89368:CDS | 40.0% | |
| AATCGTAGAGGTTAGGCAGA+AGG | + | 50654:26756-26775 | MS.gene89368:CDS | 45.0% | |
| ATCGAAACTAGGTACCTGTG+TGG | - | 50654:26573-26592 | None:intergenic | 45.0% | |
| GACAGTACTTGAGTACTTGG+TGG | + | 50654:26437-26456 | MS.gene89368:CDS | 45.0% | |
| GATTCCAGTGGAAGAGATCA+AGG | + | 50654:26676-26695 | MS.gene89368:CDS | 45.0% | |
| GCAGAAGGCTGCTGTTAATA+GGG | + | 50654:26771-26790 | MS.gene89368:CDS | 45.0% | |
| GTCGGACAAAGTCTGTTCAT+AGG | - | 50654:26644-26663 | None:intergenic | 45.0% | |
| GTTTCCTTGATCTCTTCCAC+TGG | - | 50654:26683-26702 | None:intergenic | 45.0% | |
| ACGACACTGGCAAGAATTGG+CGG | + | 50654:26266-26285 | MS.gene89368:CDS | 50.0% | |
| GAATTGGCGGCATGTCTACA+AGG | + | 50654:26279-26298 | MS.gene89368:CDS | 50.0% | |
| GGCAGAAGGCTGCTGTTAAT+AGG | + | 50654:26770-26789 | MS.gene89368:CDS | 50.0% | |
| ! | CTTGAGTACTTGGTGGCTCA+TGG | + | 50654:26444-26463 | MS.gene89368:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50654 | gene | 26241 | 26797 | 26241 | ID=MS.gene89368 |
| 50654 | mRNA | 26241 | 26797 | 26241 | ID=MS.gene89368.t1;Parent=MS.gene89368 |
| 50654 | exon | 26241 | 26300 | 26241 | ID=MS.gene89368.t1.exon1;Parent=MS.gene89368.t1 |
| 50654 | CDS | 26241 | 26300 | 26241 | ID=cds.MS.gene89368.t1;Parent=MS.gene89368.t1 |
| 50654 | exon | 26432 | 26515 | 26432 | ID=MS.gene89368.t1.exon2;Parent=MS.gene89368.t1 |
| 50654 | CDS | 26432 | 26515 | 26432 | ID=cds.MS.gene89368.t1;Parent=MS.gene89368.t1 |
| 50654 | exon | 26652 | 26797 | 26652 | ID=MS.gene89368.t1.exon3;Parent=MS.gene89368.t1 |
| 50654 | CDS | 26652 | 26797 | 26652 | ID=cds.MS.gene89368.t1;Parent=MS.gene89368.t1 |
| Gene Sequence |
| Protein sequence |
