Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94810.t1 | XP_013468395.1 | 97.4 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 2.40E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94810.t1 | Q9SVU0 | 72.4 | 424 | 112 | 2 | 1 | 419 | 1 | 424 | 7.3e-183 | 641.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94810.t1 | A0A072VKW7 | 97.4 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 1.7e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene94810 | MS.gene06418 | PPI |
| MS.gene94810 | MS.gene021474 | PPI |
| MS.gene94810 | MS.gene44332 | PPI |
| MS.gene94810 | MS.gene53594 | PPI |
| MS.gene94810 | MS.gene51580 | PPI |
| MS.gene94810 | MS.gene014685 | PPI |
| MS.gene94810 | MS.gene31616 | PPI |
| MS.gene94810 | MS.gene049295 | PPI |
| MS.gene94810 | MS.gene73119 | PPI |
| MS.gene94810 | MS.gene054944 | PPI |
| MS.gene94810 | MS.gene40340 | PPI |
| MS.gene94810 | MS.gene013322 | PPI |
| MS.gene94810 | MS.gene60245 | PPI |
| MS.gene94810 | MS.gene57685 | PPI |
| MS.gene94810 | MS.gene031178 | PPI |
| MS.gene94810 | MS.gene29205 | PPI |
| MS.gene94810 | MS.gene64265 | PPI |
| MS.gene94810 | MS.gene56048 | PPI |
| MS.gene94810 | MS.gene035805 | PPI |
| MS.gene94810 | MS.gene015953 | PPI |
| MS.gene94810 | MS.gene36879 | PPI |
| MS.gene94810 | MS.gene019484 | PPI |
| MS.gene94810 | MS.gene027681 | PPI |
| MS.gene94810 | MS.gene064951 | PPI |
| MS.gene94810 | MS.gene064542 | PPI |
| MS.gene94810 | MS.gene44331 | PPI |
| MS.gene94810 | MS.gene86173 | PPI |
| MS.gene94810 | MS.gene32792 | PPI |
| MS.gene94810 | MS.gene61665 | PPI |
| MS.gene94810 | MS.gene059136 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94810.t1 | MTR_1g069275 | 97.387 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 0.0 | 840 |
| MS.gene94810.t1 | MTR_7g099330 | 78.385 | 421 | 85 | 3 | 1 | 420 | 1 | 416 | 0.0 | 692 |
| MS.gene94810.t1 | MTR_1g046230 | 72.530 | 415 | 110 | 1 | 10 | 420 | 9 | 423 | 0.0 | 626 |
| MS.gene94810.t1 | MTR_7g099160 | 77.204 | 329 | 70 | 2 | 93 | 420 | 213 | 537 | 0.0 | 528 |
| MS.gene94810.t1 | MTR_1g008380 | 63.753 | 389 | 139 | 2 | 18 | 404 | 35 | 423 | 0.0 | 523 |
| MS.gene94810.t1 | MTR_3g109520 | 60.050 | 403 | 158 | 2 | 4 | 403 | 18 | 420 | 0.0 | 511 |
| MS.gene94810.t1 | MTR_6g086870 | 61.026 | 390 | 145 | 4 | 22 | 405 | 17 | 405 | 1.60e-178 | 504 |
| MS.gene94810.t1 | MTR_1g011630 | 59.162 | 382 | 154 | 1 | 18 | 399 | 9 | 388 | 2.40e-178 | 503 |
| MS.gene94810.t1 | MTR_7g107710 | 47.105 | 380 | 192 | 4 | 26 | 401 | 7 | 381 | 7.02e-123 | 362 |
| MS.gene94810.t1 | MTR_8g432640 | 47.733 | 375 | 187 | 4 | 26 | 396 | 7 | 376 | 2.07e-120 | 355 |
| MS.gene94810.t1 | MTR_7g107690 | 44.327 | 379 | 202 | 4 | 26 | 400 | 9 | 382 | 7.80e-120 | 354 |
| MS.gene94810.t1 | MTR_5g033260 | 46.842 | 380 | 187 | 5 | 26 | 398 | 7 | 378 | 8.98e-120 | 353 |
| MS.gene94810.t1 | MTR_3g088925 | 44.267 | 375 | 198 | 5 | 26 | 395 | 8 | 376 | 8.18e-117 | 347 |
| MS.gene94810.t1 | MTR_3g088945 | 44.000 | 375 | 199 | 5 | 26 | 395 | 8 | 376 | 8.74e-116 | 344 |
| MS.gene94810.t1 | MTR_3g088955 | 43.467 | 375 | 201 | 5 | 26 | 395 | 8 | 376 | 8.43e-114 | 339 |
| MS.gene94810.t1 | MTR_4g051642 | 45.067 | 375 | 194 | 6 | 26 | 395 | 8 | 375 | 1.76e-113 | 338 |
| MS.gene94810.t1 | MTR_3g096030 | 38.068 | 176 | 83 | 2 | 26 | 199 | 7 | 158 | 8.31e-37 | 132 |
| MS.gene94810.t1 | MTR_2g450510 | 28.632 | 234 | 129 | 8 | 27 | 223 | 7 | 239 | 1.43e-14 | 75.9 |
| MS.gene94810.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 1.81e-14 | 73.6 |
| MS.gene94810.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 2.53e-14 | 73.9 |
| MS.gene94810.t1 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 3.54e-14 | 73.9 |
| MS.gene94810.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 3.80e-14 | 74.3 |
| MS.gene94810.t1 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 6.65e-14 | 73.6 |
| MS.gene94810.t1 | MTR_2g044140 | 26.757 | 370 | 207 | 14 | 27 | 336 | 16 | 381 | 3.04e-12 | 68.6 |
| MS.gene94810.t1 | MTR_2g043850 | 25.806 | 372 | 208 | 16 | 27 | 336 | 16 | 381 | 2.56e-11 | 65.9 |
| MS.gene94810.t1 | MTR_2g020630 | 25.417 | 240 | 138 | 9 | 27 | 227 | 16 | 253 | 3.56e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94810.t1 | AT4G28720 | 72.406 | 424 | 112 | 2 | 1 | 419 | 1 | 424 | 0.0 | 649 |
| MS.gene94810.t1 | AT1G04180 | 70.883 | 419 | 121 | 1 | 1 | 418 | 1 | 419 | 0.0 | 624 |
| MS.gene94810.t1 | AT1G04610 | 69.690 | 419 | 122 | 1 | 7 | 420 | 19 | 437 | 0.0 | 615 |
| MS.gene94810.t1 | AT5G43890 | 68.957 | 422 | 127 | 2 | 1 | 418 | 1 | 422 | 0.0 | 613 |
| MS.gene94810.t1 | AT2G33230 | 69.807 | 414 | 119 | 2 | 12 | 420 | 19 | 431 | 0.0 | 584 |
| MS.gene94810.t1 | AT5G25620 | 60.494 | 405 | 156 | 3 | 6 | 406 | 12 | 416 | 2.01e-179 | 506 |
| MS.gene94810.t1 | AT5G25620 | 60.750 | 400 | 153 | 3 | 6 | 401 | 12 | 411 | 2.08e-178 | 504 |
| MS.gene94810.t1 | AT4G32540 | 55.257 | 409 | 176 | 4 | 10 | 417 | 7 | 409 | 1.97e-172 | 489 |
| MS.gene94810.t1 | AT5G11320 | 59.843 | 381 | 149 | 1 | 20 | 400 | 12 | 388 | 2.25e-172 | 488 |
| MS.gene94810.t1 | AT4G13260 | 60.789 | 380 | 146 | 2 | 18 | 394 | 21 | 400 | 2.11e-171 | 486 |
| MS.gene94810.t1 | AT4G32540 | 55.231 | 411 | 175 | 5 | 10 | 417 | 7 | 411 | 9.87e-171 | 484 |
| MS.gene94810.t1 | AT5G11320 | 59.202 | 326 | 129 | 1 | 20 | 345 | 12 | 333 | 1.53e-142 | 410 |
| MS.gene94810.t1 | AT4G13260 | 61.379 | 290 | 110 | 1 | 18 | 305 | 21 | 310 | 9.04e-132 | 382 |
| MS.gene94810.t1 | AT1G48910 | 47.215 | 377 | 188 | 5 | 26 | 397 | 6 | 376 | 6.31e-126 | 369 |
| MS.gene94810.t1 | AT1G21430 | 48.396 | 374 | 181 | 5 | 26 | 394 | 10 | 376 | 4.01e-123 | 362 |
| MS.gene94810.t1 | AT4G13260 | 58.131 | 289 | 118 | 2 | 109 | 394 | 9 | 297 | 1.08e-114 | 338 |
| MS.gene94810.t1 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 2.64e-19 | 89.7 |
| MS.gene94810.t1 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 5.20e-19 | 89.4 |
| MS.gene94810.t1 | AT5G45180 | 26.136 | 352 | 192 | 14 | 27 | 336 | 15 | 340 | 2.10e-17 | 84.3 |
| MS.gene94810.t1 | AT1G65860 | 25.561 | 223 | 138 | 4 | 27 | 221 | 15 | 237 | 3.07e-16 | 80.9 |
| MS.gene94810.t1 | AT1G12160 | 26.636 | 214 | 124 | 6 | 27 | 209 | 14 | 225 | 1.14e-15 | 79.3 |
| MS.gene94810.t1 | AT5G07800 | 26.147 | 218 | 125 | 5 | 27 | 208 | 18 | 235 | 1.89e-15 | 78.6 |
| MS.gene94810.t1 | AT1G62620 | 25.926 | 243 | 138 | 8 | 27 | 234 | 15 | 250 | 1.90e-15 | 78.2 |
| MS.gene94810.t1 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 3.12e-15 | 77.0 |
| MS.gene94810.t1 | AT1G62620 | 25.417 | 240 | 143 | 7 | 27 | 234 | 15 | 250 | 4.04e-15 | 77.4 |
| MS.gene94810.t1 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 7.53e-15 | 76.6 |
| MS.gene94810.t1 | AT5G61290 | 26.267 | 217 | 126 | 5 | 27 | 209 | 18 | 234 | 1.63e-14 | 75.5 |
| MS.gene94810.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.36e-14 | 74.7 |
| MS.gene94810.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.37e-14 | 74.7 |
| MS.gene94810.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.55e-14 | 74.3 |
| MS.gene94810.t1 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 5.70e-14 | 73.6 |
| MS.gene94810.t1 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 1.40e-13 | 72.8 |
| MS.gene94810.t1 | AT1G12130 | 23.451 | 226 | 142 | 5 | 41 | 235 | 29 | 254 | 5.44e-13 | 70.9 |
| MS.gene94810.t1 | AT1G62570 | 22.967 | 209 | 133 | 3 | 41 | 221 | 29 | 237 | 1.66e-12 | 69.3 |
| MS.gene94810.t1 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.08e-12 | 68.9 |
| MS.gene94810.t1 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.08e-12 | 68.9 |
| MS.gene94810.t1 | AT1G62600 | 24.464 | 233 | 126 | 7 | 34 | 219 | 22 | 251 | 9.31e-11 | 63.5 |
Find 92 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTCTTTGTTGGATATTC+AGG | 0.151113 | 1.4:+55741328 | None:intergenic |
| GTTGTTGGTTGTGGTAATTC+TGG | 0.205623 | 1.4:-55741019 | MS.gene94810:CDS |
| GGTAGTTGGCAGAATTGTTT+AGG | 0.235028 | 1.4:+55741376 | None:intergenic |
| CTCAAGATATTGCTCAAGTT+TGG | 0.269888 | 1.4:-55739794 | MS.gene94810:CDS |
| ATCGTAGCAATGTCCCTTCT+TGG | 0.282135 | 1.4:-55740297 | MS.gene94810:CDS |
| TAGAAAAGTATGGTCTTAAA+AGG | 0.282601 | 1.4:-55740510 | MS.gene94810:CDS |
| CAATTTGTGCAATTTAGAAT+TGG | 0.285416 | 1.4:+55739712 | None:intergenic |
| GGTCCTGCTCCTACTATTAT+AGG | 0.301581 | 1.4:+55741523 | None:intergenic |
| CATGCTTGTGATTATAAATC+AGG | 0.314519 | 1.4:-55741070 | MS.gene94810:CDS |
| AAAGGGTAAGACCCCTGTTT+TGG | 0.316214 | 1.4:-55740455 | MS.gene94810:CDS |
| TTACTCAACCCTTCAATATC+AGG | 0.327104 | 1.4:+55741112 | None:intergenic |
| GGCTGTGTTGAACTTGTTAA+TGG | 0.328799 | 1.4:-55740361 | MS.gene94810:CDS |
| GATAGTTGACAAGATTCTTT+TGG | 0.331991 | 1.4:-55740560 | MS.gene94810:CDS |
| GCTAAATATGATGAGACTAA+TGG | 0.339410 | 1.4:-55741232 | MS.gene94810:CDS |
| TTTATGAACTGTTTCTTTGT+TGG | 0.340000 | 1.4:+55741319 | None:intergenic |
| CAGTTCCTTTCCAACCATTA+GGG | 0.343203 | 1.4:+55739886 | None:intergenic |
| TAGTCTCACATCGTTGCATT+TGG | 0.343936 | 1.4:-55741555 | MS.gene94810:CDS |
| AAGGCCATTGGAGGGTCCTT+TGG | 0.345490 | 1.4:-55740491 | MS.gene94810:CDS |
| ACAGTTCCTTTCCAACCATT+AGG | 0.345992 | 1.4:+55739885 | None:intergenic |
| CAATGTCCCTTCTTGGCTTA+AGG | 0.348481 | 1.4:-55740290 | MS.gene94810:intron |
| GTAGTTGGCAGAATTGTTTA+GGG | 0.348558 | 1.4:+55741377 | None:intergenic |
| TGACTTTGGAAATCCATTCT+TGG | 0.361539 | 1.4:+55739914 | None:intergenic |
| CCAAAGTCACCATTCCCTAA+TGG | 0.366181 | 1.4:-55739900 | MS.gene94810:CDS |
| TTAGTAAACCTCTTAATTCC+AGG | 0.368388 | 1.4:+55740385 | None:intergenic |
| TCTTGTGGGAATGGAAAGTT+AGG | 0.369111 | 1.4:+55741355 | None:intergenic |
| CTGTTGGACTTTATGCTGTT+GGG | 0.378354 | 1.4:-55739866 | MS.gene94810:CDS |
| CATCGTTGCATTTGGGTAAA+CGG | 0.385627 | 1.4:-55741547 | MS.gene94810:CDS |
| AATTTGTGCAATTTAGAATT+GGG | 0.388229 | 1.4:+55739713 | None:intergenic |
| AAGGTGCCAATATCCAAAAC+AGG | 0.397457 | 1.4:+55740442 | None:intergenic |
| ATACTCAACTTCATTTCCAT+TGG | 0.414194 | 1.4:+55741186 | None:intergenic |
| AGTCTCACATCGTTGCATTT+GGG | 0.415427 | 1.4:-55741554 | MS.gene94810:CDS |
| TTTGGTAATATAGAAAAGTA+TGG | 0.417326 | 1.4:-55740520 | MS.gene94810:CDS |
| TGATGAAATGGTTACCCGTT+TGG | 0.423835 | 1.4:-55740582 | MS.gene94810:CDS |
| AGGTCGCTTGTGGTTGCTAC+TGG | 0.431631 | 1.4:-55741157 | MS.gene94810:CDS |
| GATGCAGTTGTTCTTGCTAC+TGG | 0.439635 | 1.4:-55740322 | MS.gene94810:CDS |
| CCATTAGGGAATGGTGACTT+TGG | 0.441458 | 1.4:+55739900 | None:intergenic |
| CAAATTGCATAGCTTCACTA+TGG | 0.459530 | 1.4:-55741435 | MS.gene94810:CDS |
| ACAACAAGGAGTACCCTTCA+TGG | 0.462402 | 1.4:-55741470 | MS.gene94810:CDS |
| GTCTATTGTTCTTCGTAGCT+CGG | 0.464587 | 1.4:-55740954 | MS.gene94810:intron |
| TCAGGAAATTCTTGTGGGAA+TGG | 0.465607 | 1.4:+55741346 | None:intergenic |
| ATGAAGTTGAGTATATTTGT+AGG | 0.466018 | 1.4:-55741177 | MS.gene94810:CDS |
| GATATTCAGGAAATTCTTGT+GGG | 0.470627 | 1.4:+55741341 | None:intergenic |
| ACTGTTGGACTTTATGCTGT+TGG | 0.470697 | 1.4:-55739867 | MS.gene94810:CDS |
| ATAGTAGGAGCAGGACCATC+AGG | 0.478379 | 1.4:-55741517 | MS.gene94810:CDS |
| TGGCAGTTATGATGATGAAA+TGG | 0.484448 | 1.4:-55740594 | MS.gene94810:CDS |
| TTTACAAAGAGAGGGCTCTC+TGG | 0.487445 | 1.4:-55739843 | MS.gene94810:CDS |
| AATCTTGTCAACTATCCAAA+CGG | 0.487573 | 1.4:+55740567 | None:intergenic |
| TGGGAAATCAACATTTGGAT+TGG | 0.487762 | 1.4:-55740614 | MS.gene94810:CDS |
| GGAATTAAGAGGTTTACTAA+TGG | 0.490761 | 1.4:-55740382 | MS.gene94810:CDS |
| ATCTTGTCAACTATCCAAAC+GGG | 0.505639 | 1.4:+55740568 | None:intergenic |
| GAACACACTCGTTGAATCGC+GGG | 0.507960 | 1.4:+55741260 | None:intergenic |
| TTGGAGCTTAAGAACACAAA+GGG | 0.508263 | 1.4:-55740472 | MS.gene94810:CDS |
| GTAAACGGTCCTATAATAGT+AGG | 0.514321 | 1.4:-55741532 | MS.gene94810:CDS |
| TGATCTTTCAAGCACCATGA+AGG | 0.516098 | 1.4:+55741456 | None:intergenic |
| AAGTTGTGCCTGGAATTAAG+AGG | 0.518103 | 1.4:-55740393 | MS.gene94810:CDS |
| GGATATTCAGGAAATTCTTG+TGG | 0.520358 | 1.4:+55741340 | None:intergenic |
| CCTTTCCAACCATTAGGGAA+TGG | 0.520534 | 1.4:+55739891 | None:intergenic |
| TTTCTCACCTTAAGCCAAGA+AGG | 0.523569 | 1.4:+55740283 | None:intergenic |
| ACCTTGGAGAAAATTAGATC+TGG | 0.532203 | 1.4:-55740424 | MS.gene94810:CDS |
| GTATGGTCTTAAAAGGCCAT+TGG | 0.535113 | 1.4:-55740503 | MS.gene94810:CDS |
| GCTGTTGGGTTTACAAAGAG+AGG | 0.540106 | 1.4:-55739852 | MS.gene94810:CDS |
| TTTGTGTTCTTAAGCTCCAA+AGG | 0.541522 | 1.4:+55740475 | None:intergenic |
| TGTGATTATAAATCAGGTGA+AGG | 0.541872 | 1.4:-55741064 | MS.gene94810:CDS |
| GGAATGGAAAGTTAGGTAGT+TGG | 0.544827 | 1.4:+55741362 | None:intergenic |
| TGGTCTTAAAAGGCCATTGG+AGG | 0.553703 | 1.4:-55740500 | MS.gene94810:CDS |
| TGTGTTGTGCCTGATATTGA+AGG | 0.554440 | 1.4:-55741121 | MS.gene94810:CDS |
| GCTGCTGTTGCTAGACCTGA+TGG | 0.555064 | 1.4:+55741502 | None:intergenic |
| AATGGTTGGAAAGGAACTGT+TGG | 0.555476 | 1.4:-55739882 | MS.gene94810:CDS |
| AGTCACCATTCCCTAATGGT+TGG | 0.557817 | 1.4:-55739896 | MS.gene94810:CDS |
| CCATTCCCTAATGGTTGGAA+AGG | 0.559102 | 1.4:-55739891 | MS.gene94810:CDS |
| GGTCACATAAAAGTTGTGCC+TGG | 0.561332 | 1.4:-55740403 | MS.gene94810:CDS |
| TGAACACACTCGTTGAATCG+CGG | 0.564121 | 1.4:+55741259 | None:intergenic |
| TGGTTGTGGTAATTCTGGAA+TGG | 0.564985 | 1.4:-55741014 | MS.gene94810:CDS |
| CTGTTGGGTTTACAAAGAGA+GGG | 0.568492 | 1.4:-55739851 | MS.gene94810:CDS |
| ATGATGAGACTAATGGATTG+TGG | 0.574427 | 1.4:-55741225 | MS.gene94810:CDS |
| GATCTTTCAAGCACCATGAA+GGG | 0.575566 | 1.4:+55741457 | None:intergenic |
| TATCTTCTTCATTATCGACT+AGG | 0.583142 | 1.4:+55741578 | None:intergenic |
| TTCTCACCTTAAGCCAAGAA+GGG | 0.584034 | 1.4:+55740284 | None:intergenic |
| GTTGGCAGAATTGTTTAGGG+AGG | 0.587889 | 1.4:+55741380 | None:intergenic |
| GAAGGGTTGAGTAAATTCAA+AGG | 0.589620 | 1.4:-55741103 | MS.gene94810:CDS |
| GATTTCAAAGTGTTTCGCGT+AGG | 0.592046 | 1.4:+55741285 | None:intergenic |
| AGCTCCAAAGGACCCTCCAA+TGG | 0.594886 | 1.4:+55740487 | None:intergenic |
| GGTCCTATAATAGTAGGAGC+AGG | 0.599245 | 1.4:-55741526 | MS.gene94810:CDS |
| GTATATTTGTAGGTCGCTTG+TGG | 0.619170 | 1.4:-55741167 | MS.gene94810:CDS |
| AGAGTTGAGACAGTTTCCAA+TGG | 0.619735 | 1.4:-55741202 | MS.gene94810:CDS |
| AGGTGCCAATATCCAAAACA+GGG | 0.629075 | 1.4:+55740443 | None:intergenic |
| GTGTTGTGCCTGATATTGAA+GGG | 0.636186 | 1.4:-55741120 | MS.gene94810:CDS |
| GGTCTTAAAAGGCCATTGGA+GGG | 0.661835 | 1.4:-55740499 | MS.gene94810:CDS |
| TTTGGAGCTTAAGAACACAA+AGG | 0.673099 | 1.4:-55740473 | MS.gene94810:CDS |
| GAGCTACGAAGAACAATAGA+CGG | 0.679802 | 1.4:+55740956 | None:intergenic |
| GCAGCATGTCTTAAACAACA+AGG | 0.727523 | 1.4:-55741484 | MS.gene94810:CDS |
| GGTGCCAATATCCAAAACAG+GGG | 0.738091 | 1.4:+55740444 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTAAGAAAAATAATTTG+AGG | - | chr1.4:55740600-55740619 | MS.gene94810:CDS | 10.0% |
| !!! | GATGATGTTTTTTTTTTTTT+TGG | - | chr1.4:55740622-55740641 | MS.gene94810:CDS | 15.0% |
| !!! | GTTAATAACTAATCTTATTT+TGG | - | chr1.4:55741350-55741369 | MS.gene94810:CDS | 15.0% |
| !!! | TTTTTTTTTGGTACTAATTA+AGG | - | chr1.4:55740634-55740653 | MS.gene94810:CDS | 15.0% |
| !!! | AGTATTTTACTATTTTTGTG+TGG | - | chr1.4:55741326-55741345 | MS.gene94810:CDS | 20.0% |
| !!! | TTTGGTAATATAGAAAAGTA+TGG | - | chr1.4:55740800-55740819 | MS.gene94810:intron | 20.0% |
| ! | ATACTAATTCCATTTCCAAA+AGG | + | chr1.4:55741311-55741330 | None:intergenic | 25.0% |
| ! | ATATGATAAGACCCAAAAAA+AGG | + | chr1.4:55741207-55741226 | None:intergenic | 25.0% |
| ! | ATGAAGTTGAGTATATTTGT+AGG | - | chr1.4:55740143-55740162 | MS.gene94810:intron | 25.0% |
| ! | TACTAATTCCATTTCCAAAA+GGG | + | chr1.4:55741310-55741329 | None:intergenic | 25.0% |
| !! | AATTAAGGTTGTTGTGTTTT+TGG | - | chr1.4:55740649-55740668 | MS.gene94810:intron | 25.0% |
| !! | TAGAAAAGTATGGTCTTAAA+AGG | - | chr1.4:55740810-55740829 | MS.gene94810:intron | 25.0% |
| !! | TTTATGAACTGTTTCTTTGT+TGG | + | chr1.4:55740004-55740023 | None:intergenic | 25.0% |
| !!! | TGGTTTTGAAACCTTTTTTT+GGG | - | chr1.4:55741193-55741212 | MS.gene94810:CDS | 25.0% |
| !!! | TTGGTTTTGAAACCTTTTTT+TGG | - | chr1.4:55741192-55741211 | MS.gene94810:CDS | 25.0% |
| !!! | TTGTGATTTTGTTTATTTCG+AGG | - | chr1.4:55740557-55740576 | MS.gene94810:CDS | 25.0% |
| AATCTTGTCAACTATCCAAA+CGG | + | chr1.4:55740756-55740775 | None:intergenic | 30.0% | |
| ACTAATTCCATTTCCAAAAG+GGG | + | chr1.4:55741309-55741328 | None:intergenic | 30.0% | |
| ATACTCAACTTCATTTCCAT+TGG | + | chr1.4:55740137-55740156 | None:intergenic | 30.0% | |
| CATGCTTGTGATTATAAATC+AGG | - | chr1.4:55740250-55740269 | MS.gene94810:intron | 30.0% | |
| GATATTCAGGAAATTCTTGT+GGG | + | chr1.4:55739982-55740001 | None:intergenic | 30.0% | |
| GCTAAATATGATGAGACTAA+TGG | - | chr1.4:55740088-55740107 | MS.gene94810:intron | 30.0% | |
| GGAATTAAGAGGTTTACTAA+TGG | - | chr1.4:55740938-55740957 | MS.gene94810:intron | 30.0% | |
| TGCAAATTTGATGTTTGGTT+CGG | - | chr1.4:55741156-55741175 | MS.gene94810:CDS | 30.0% | |
| TGTGATTATAAATCAGGTGA+AGG | - | chr1.4:55740256-55740275 | MS.gene94810:intron | 30.0% | |
| TGTTTCTTTGTTGGATATTC+AGG | + | chr1.4:55739995-55740014 | None:intergenic | 30.0% | |
| TTAGTAAACCTCTTAATTCC+AGG | + | chr1.4:55740938-55740957 | None:intergenic | 30.0% | |
| ! | GATAGTTGACAAGATTCTTT+TGG | - | chr1.4:55740760-55740779 | MS.gene94810:intron | 30.0% |
| ! | TATCTTCTTCATTATCGACT+AGG | + | chr1.4:55739745-55739764 | None:intergenic | 30.0% |
| ! | TTTTGCCTAGAGAAGTTTTT+GGG | - | chr1.4:55740687-55740706 | MS.gene94810:intron | 30.0% |
| ! | TTTTTACCTTTTTCCCCTTT+TGG | - | chr1.4:55741293-55741312 | MS.gene94810:CDS | 30.0% |
| !! | GTTTTTGGGAAATCAACATT+TGG | - | chr1.4:55740701-55740720 | MS.gene94810:intron | 30.0% |
| !! | TTTGGTTCATTTTTGTGTGT+AGG | - | chr1.4:55741368-55741387 | MS.gene94810:CDS | 30.0% |
| !! | TTTTAGAGGGAAGAAAGTTT+TGG | - | chr1.4:55740279-55740298 | MS.gene94810:intron | 30.0% |
| !!! | GGTCGAAGATTTTTAAGTTT+GGG | - | chr1.4:55740527-55740546 | MS.gene94810:CDS | 30.0% |
| !!! | GTCGAAGATTTTTAAGTTTG+GGG | - | chr1.4:55740528-55740547 | MS.gene94810:CDS | 30.0% |
| !!! | TGACAAGATTCTTTTGGTTT+TGG | - | chr1.4:55740766-55740785 | MS.gene94810:intron | 30.0% |
| !!! | TGGTCGAAGATTTTTAAGTT+TGG | - | chr1.4:55740526-55740545 | MS.gene94810:CDS | 30.0% |
| ACAGTTGAACCATGATTCAT+AGG | - | chr1.4:55741130-55741149 | MS.gene94810:CDS | 35.0% | |
| ACCTTGGAGAAAATTAGATC+TGG | - | chr1.4:55740896-55740915 | MS.gene94810:intron | 35.0% | |
| ATCTTGTCAACTATCCAAAC+GGG | + | chr1.4:55740755-55740774 | None:intergenic | 35.0% | |
| CAAATTGCATAGCTTCACTA+TGG | - | chr1.4:55739885-55739904 | MS.gene94810:CDS | 35.0% | |
| CTCAAGATATTGCTCAAGTT+TGG | - | chr1.4:55741526-55741545 | MS.gene94810:CDS | 35.0% | |
| GAAGGGTTGAGTAAATTCAA+AGG | - | chr1.4:55740217-55740236 | MS.gene94810:intron | 35.0% | |
| GATTTCCCAAAAACTTCTCT+AGG | + | chr1.4:55740695-55740714 | None:intergenic | 35.0% | |
| GGATATTCAGGAAATTCTTG+TGG | + | chr1.4:55739983-55740002 | None:intergenic | 35.0% | |
| GGTCTTGCAAATTTGATGTT+TGG | - | chr1.4:55741151-55741170 | MS.gene94810:CDS | 35.0% | |
| GTAAACGGTCCTATAATAGT+AGG | - | chr1.4:55739788-55739807 | MS.gene94810:CDS | 35.0% | |
| GTAGTTGGCAGAATTGTTTA+GGG | + | chr1.4:55739946-55739965 | None:intergenic | 35.0% | |
| TGGGAAATCAACATTTGGAT+TGG | - | chr1.4:55740706-55740725 | MS.gene94810:intron | 35.0% | |
| TTACTCAACCCTTCAATATC+AGG | + | chr1.4:55740211-55740230 | None:intergenic | 35.0% | |
| TTGGAGCTTAAGAACACAAA+GGG | - | chr1.4:55740848-55740867 | MS.gene94810:intron | 35.0% | |
| TTTGCAAGACCTATGAATCA+TGG | + | chr1.4:55741142-55741161 | None:intergenic | 35.0% | |
| TTTGTGTTCTTAAGCTCCAA+AGG | + | chr1.4:55740848-55740867 | None:intergenic | 35.0% | |
| ! | ACCAGATCTAATTTTCTCCA+AGG | + | chr1.4:55740900-55740919 | None:intergenic | 35.0% |
| ! | GGTGAATTTTTCTCCAAGAA+TGG | - | chr1.4:55741393-55741412 | MS.gene94810:CDS | 35.0% |
| ! | GTTTTGCCTAGAGAAGTTTT+TGG | - | chr1.4:55740686-55740705 | MS.gene94810:intron | 35.0% |
| ! | TGACTTTGGAAATCCATTCT+TGG | + | chr1.4:55741409-55741428 | None:intergenic | 35.0% |
| ! | TGGCAGTTATGATGATGAAA+TGG | - | chr1.4:55740726-55740745 | MS.gene94810:intron | 35.0% |
| ! | TTTGGAGCTTAAGAACACAA+AGG | - | chr1.4:55740847-55740866 | MS.gene94810:intron | 35.0% |
| !! | AAATCAGGTGAAGGTTTTAG+AGG | - | chr1.4:55740265-55740284 | MS.gene94810:intron | 35.0% |
| !! | ATGATGAGACTAATGGATTG+TGG | - | chr1.4:55740095-55740114 | MS.gene94810:intron | 35.0% |
| !! | GTTCATTTTTGTGTGTAGGA+AGG | - | chr1.4:55741372-55741391 | MS.gene94810:CDS | 35.0% |
| !! | TTCAACTGTTTTGAACTGCT+CGG | + | chr1.4:55741119-55741138 | None:intergenic | 35.0% |
| !! | TTGCTCTGAAAGAACTAATG+TGG | - | chr1.4:55740506-55740525 | MS.gene94810:CDS | 35.0% |
| !!! | AAAGTTTTGGTTGTTGGTTG+TGG | - | chr1.4:55740292-55740311 | MS.gene94810:CDS | 35.0% |
| !!! | AATCAGGTGAAGGTTTTAGA+GGG | - | chr1.4:55740266-55740285 | MS.gene94810:intron | 35.0% |
| !!! | AGATTCTTTTGGTTTTGGCT+TGG | - | chr1.4:55740771-55740790 | MS.gene94810:intron | 35.0% |
| !!! | TGTTGTGTTTTTGGTTGAAC+AGG | - | chr1.4:55740658-55740677 | MS.gene94810:intron | 35.0% |
| AAGTTGTGCCTGGAATTAAG+AGG | - | chr1.4:55740927-55740946 | MS.gene94810:intron | 40.0% | |
| AATGGTTGGAAAGGAACTGT+TGG | - | chr1.4:55741438-55741457 | MS.gene94810:CDS | 40.0% | |
| ACAGTTCCTTTCCAACCATT+AGG | + | chr1.4:55741438-55741457 | None:intergenic | 40.0% | |
| ACTGTTGGACTTTATGCTGT+TGG | - | chr1.4:55741453-55741472 | MS.gene94810:CDS | 40.0% | |
| AGAGTTGAGACAGTTTCCAA+TGG | - | chr1.4:55740118-55740137 | MS.gene94810:intron | 40.0% | |
| AGGTGCCAATATCCAAAACA+GGG | + | chr1.4:55740880-55740899 | None:intergenic | 40.0% | |
| AGTCTCACATCGTTGCATTT+GGG | - | chr1.4:55739766-55739785 | MS.gene94810:CDS | 40.0% | |
| CAGTTCCTTTCCAACCATTA+GGG | + | chr1.4:55741437-55741456 | None:intergenic | 40.0% | |
| CATCGTTGCATTTGGGTAAA+CGG | - | chr1.4:55739773-55739792 | MS.gene94810:CDS | 40.0% | |
| CCATTTCCAAAAGGGGAAAA+AGG | + | chr1.4:55741302-55741321 | None:intergenic | 40.0% | |
| CTGTTGGACTTTATGCTGTT+GGG | - | chr1.4:55741454-55741473 | MS.gene94810:CDS | 40.0% | |
| CTGTTGGGTTTACAAAGAGA+GGG | - | chr1.4:55741469-55741488 | MS.gene94810:CDS | 40.0% | |
| GAGCTACGAAGAACAATAGA+CGG | + | chr1.4:55740367-55740386 | None:intergenic | 40.0% | |
| GATCTTTCAAGCACCATGAA+GGG | + | chr1.4:55739866-55739885 | None:intergenic | 40.0% | |
| GCAGCATGTCTTAAACAACA+AGG | - | chr1.4:55739836-55739855 | MS.gene94810:CDS | 40.0% | |
| GGAATGGAAAGTTAGGTAGT+TGG | + | chr1.4:55739961-55739980 | None:intergenic | 40.0% | |
| GGTAGTTGGCAGAATTGTTT+AGG | + | chr1.4:55739947-55739966 | None:intergenic | 40.0% | |
| GTATATTTGTAGGTCGCTTG+TGG | - | chr1.4:55740153-55740172 | MS.gene94810:intron | 40.0% | |
| GTCTATTGTTCTTCGTAGCT+CGG | - | chr1.4:55740366-55740385 | MS.gene94810:CDS | 40.0% | |
| GTGTTGTGCCTGATATTGAA+GGG | - | chr1.4:55740200-55740219 | MS.gene94810:intron | 40.0% | |
| TAGTCTCACATCGTTGCATT+TGG | - | chr1.4:55739765-55739784 | MS.gene94810:CDS | 40.0% | |
| TCAGGAAATTCTTGTGGGAA+TGG | + | chr1.4:55739977-55739996 | None:intergenic | 40.0% | |
| TCTTGTGGGAATGGAAAGTT+AGG | + | chr1.4:55739968-55739987 | None:intergenic | 40.0% | |
| TGATCTTTCAAGCACCATGA+AGG | + | chr1.4:55739867-55739886 | None:intergenic | 40.0% | |
| TGATGAAATGGTTACCCGTT+TGG | - | chr1.4:55740738-55740757 | MS.gene94810:intron | 40.0% | |
| TGTGTTGTGCCTGATATTGA+AGG | - | chr1.4:55740199-55740218 | MS.gene94810:intron | 40.0% | |
| TTCTCACCTTAAGCCAAGAA+GGG | + | chr1.4:55741039-55741058 | None:intergenic | 40.0% | |
| TTTCTCACCTTAAGCCAAGA+AGG | + | chr1.4:55741040-55741059 | None:intergenic | 40.0% | |
| ! | AAGGTGCCAATATCCAAAAC+AGG | + | chr1.4:55740881-55740900 | None:intergenic | 40.0% |
| ! | GATTTCAAAGTGTTTCGCGT+AGG | + | chr1.4:55740038-55740057 | None:intergenic | 40.0% |
| ! | GTATGGTCTTAAAAGGCCAT+TGG | - | chr1.4:55740817-55740836 | MS.gene94810:intron | 40.0% |
| ! | GTTGTTGGTTGTGGTAATTC+TGG | - | chr1.4:55740301-55740320 | MS.gene94810:CDS | 40.0% |
| ! | TGGTTGTGGTAATTCTGGAA+TGG | - | chr1.4:55740306-55740325 | MS.gene94810:CDS | 40.0% |
| !! | AAGACCCCTGTTTTGGATAT+TGG | - | chr1.4:55740872-55740891 | MS.gene94810:intron | 40.0% |
| !! | CCTTTTTCCCCTTTTGGAAA+TGG | - | chr1.4:55741299-55741318 | MS.gene94810:CDS | 40.0% |
| !! | GGCTGTGTTGAACTTGTTAA+TGG | - | chr1.4:55740959-55740978 | MS.gene94810:CDS | 40.0% |
| !! | TGTTTTGGATATTGGCACCT+TGG | - | chr1.4:55740880-55740899 | MS.gene94810:intron | 40.0% |
| !!! | GGGAAGAAAGTTTTGGTTGT+TGG | - | chr1.4:55740286-55740305 | MS.gene94810:intron | 40.0% |
| !!! | GTTCGGTTTTGAAACATCGT+TGG | - | chr1.4:55741173-55741192 | MS.gene94810:CDS | 40.0% |
| !!! | GTTTTGGCTTGGTTAGTGTT+TGG | - | chr1.4:55740782-55740801 | MS.gene94810:intron | 40.0% |
| AGTCACCATTCCCTAATGGT+TGG | - | chr1.4:55741424-55741443 | MS.gene94810:CDS | 45.0% | |
| ATCGTAGCAATGTCCCTTCT+TGG | - | chr1.4:55741023-55741042 | MS.gene94810:CDS | 45.0% | |
| CAATGTCCCTTCTTGGCTTA+AGG | - | chr1.4:55741030-55741049 | MS.gene94810:CDS | 45.0% | |
| CCAAAGTCACCATTCCCTAA+TGG | - | chr1.4:55741420-55741439 | MS.gene94810:CDS | 45.0% | |
| CCATTAGGGAATGGTGACTT+TGG | + | chr1.4:55741423-55741442 | None:intergenic | 45.0% | |
| CCATTCCCTAATGGTTGGAA+AGG | - | chr1.4:55741429-55741448 | MS.gene94810:CDS | 45.0% | |
| CCTTTCCAACCATTAGGGAA+TGG | + | chr1.4:55741432-55741451 | None:intergenic | 45.0% | |
| GATGCAGTTGTTCTTGCTAC+TGG | - | chr1.4:55740998-55741017 | MS.gene94810:CDS | 45.0% | |
| GCTGTTGGGTTTACAAAGAG+AGG | - | chr1.4:55741468-55741487 | MS.gene94810:CDS | 45.0% | |
| GGTCACATAAAAGTTGTGCC+TGG | - | chr1.4:55740917-55740936 | MS.gene94810:intron | 45.0% | |
| GGTCCTATAATAGTAGGAGC+AGG | - | chr1.4:55739794-55739813 | MS.gene94810:CDS | 45.0% | |
| GGTCCTGCTCCTACTATTAT+AGG | + | chr1.4:55739800-55739819 | None:intergenic | 45.0% | |
| GGTCTTAAAAGGCCATTGGA+GGG | - | chr1.4:55740821-55740840 | MS.gene94810:intron | 45.0% | |
| GGTGCCAATATCCAAAACAG+GGG | + | chr1.4:55740879-55740898 | None:intergenic | 45.0% | |
| GTTGGCAGAATTGTTTAGGG+AGG | + | chr1.4:55739943-55739962 | None:intergenic | 45.0% | |
| TGAACACACTCGTTGAATCG+CGG | + | chr1.4:55740064-55740083 | None:intergenic | 45.0% | |
| TTTACAAAGAGAGGGCTCTC+TGG | - | chr1.4:55741477-55741496 | MS.gene94810:CDS | 45.0% | |
| ! | AAAGGGTAAGACCCCTGTTT+TGG | - | chr1.4:55740865-55740884 | MS.gene94810:intron | 45.0% |
| ! | TGGTCTTAAAAGGCCATTGG+AGG | - | chr1.4:55740820-55740839 | MS.gene94810:intron | 45.0% |
| !! | ACAACAAGGAGTACCCTTCA+TGG | - | chr1.4:55739850-55739869 | MS.gene94810:CDS | 45.0% |
| !!! | AAAAAAATTATTTTATGTAT+TGG | + | chr1.4:55741246-55741265 | None:intergenic | 5.0% |
| ATAGTAGGAGCAGGACCATC+AGG | - | chr1.4:55739803-55739822 | MS.gene94810:CDS | 50.0% | |
| GAACACACTCGTTGAATCGC+GGG | + | chr1.4:55740063-55740082 | None:intergenic | 50.0% | |
| AAGGCCATTGGAGGGTCCTT+TGG | - | chr1.4:55740829-55740848 | MS.gene94810:intron | 55.0% | |
| AGCTCCAAAGGACCCTCCAA+TGG | + | chr1.4:55740836-55740855 | None:intergenic | 55.0% | |
| AGGTCGCTTGTGGTTGCTAC+TGG | - | chr1.4:55740163-55740182 | MS.gene94810:intron | 55.0% | |
| GCTGCTGTTGCTAGACCTGA+TGG | + | chr1.4:55739821-55739840 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 55739725 | 55741617 | 55739725 | ID=MS.gene94810 |
| chr1.4 | mRNA | 55739725 | 55741617 | 55739725 | ID=MS.gene94810.t1;Parent=MS.gene94810 |
| chr1.4 | exon | 55740955 | 55741617 | 55740955 | ID=MS.gene94810.t1.exon1;Parent=MS.gene94810.t1 |
| chr1.4 | CDS | 55740955 | 55741617 | 55740955 | ID=cds.MS.gene94810.t1;Parent=MS.gene94810.t1 |
| chr1.4 | exon | 55740291 | 55740662 | 55740291 | ID=MS.gene94810.t1.exon2;Parent=MS.gene94810.t1 |
| chr1.4 | CDS | 55740291 | 55740662 | 55740291 | ID=cds.MS.gene94810.t1;Parent=MS.gene94810.t1 |
| chr1.4 | exon | 55739725 | 55739952 | 55739725 | ID=MS.gene94810.t1.exon3;Parent=MS.gene94810.t1 |
| chr1.4 | CDS | 55739725 | 55739952 | 55739725 | ID=cds.MS.gene94810.t1;Parent=MS.gene94810.t1 |
| Gene Sequence |
| Protein sequence |
