AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96584


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96584.t1 MTR_7g058640 93.333 195 13 0 1 195 1 195 8.29e-138 384
MS.gene96584.t1 MTR_7g058640 95.349 172 8 0 1 172 1 172 3.02e-123 347
MS.gene96584.t1 MTR_4g022570 74.359 195 50 0 1 195 1 195 4.62e-111 317
MS.gene96584.t1 MTR_1g047720 65.258 213 74 0 1 213 1 213 9.02e-110 314
MS.gene96584.t1 MTR_7g058900 70.256 195 58 0 1 195 1 195 5.85e-104 299
MS.gene96584.t1 MTR_4g083490 66.839 193 64 0 3 195 5 197 1.45e-100 290
MS.gene96584.t1 MTR_2g436870 60.094 213 85 0 1 213 1 213 3.88e-99 287
MS.gene96584.t1 MTR_2g028790 65.285 193 67 0 3 195 7 199 3.76e-97 282
MS.gene96584.t1 MTR_1g111890 46.753 231 104 2 1 212 1 231 6.67e-79 236
MS.gene96584.t1 MTR_7g058640 94.017 117 7 0 79 195 1 117 1.00e-76 227
MS.gene96584.t1 MTR_1g047710 60.902 133 51 1 56 187 3 135 4.78e-57 177
MS.gene96584.t1 MTR_4g022470 71.028 107 31 0 64 170 10 116 1.88e-52 165
MS.gene96584.t1 MTR_5g089370 36.649 191 118 3 5 194 2 190 4.68e-38 131
MS.gene96584.t1 MTR_4g052550 32.461 191 126 3 5 194 2 190 2.01e-36 127
MS.gene96584.t1 MTR_4g115390 30.047 213 141 5 6 213 3 212 6.05e-29 108
MS.gene96584.t1 MTR_4g022550 71.053 38 11 0 158 195 10 47 1.85e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96584.t1 AT2G33120 72.308 195 54 0 1 195 1 195 1.17e-107 308
MS.gene96584.t1 AT1G04750 71.795 195 55 0 1 195 1 195 4.02e-107 307
MS.gene96584.t1 AT1G04760 68.000 200 64 0 1 200 1 200 2.48e-106 305
MS.gene96584.t1 AT1G04760 68.000 200 64 0 1 200 1 200 2.48e-106 305
MS.gene96584.t1 AT2G33120 68.966 203 55 1 1 195 1 203 1.99e-104 301
MS.gene96584.t1 AT2G32670 69.231 195 60 0 1 195 66 260 5.17e-104 301
MS.gene96584.t1 AT4G15780 60.656 183 71 1 1 182 1 183 6.09e-83 244
MS.gene96584.t1 AT2G33110 60.513 195 73 1 1 195 1 191 6.92e-83 246
MS.gene96584.t1 AT3G54300 47.210 233 100 2 1 212 1 231 6.76e-78 234
MS.gene96584.t1 AT3G54300 47.210 233 100 2 1 212 1 231 6.76e-78 234
MS.gene96584.t1 AT3G54300 47.210 233 100 2 1 212 1 231 6.76e-78 234
MS.gene96584.t1 AT2G33110 63.095 168 62 0 1 168 1 168 3.03e-76 228
MS.gene96584.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.67e-58 181
MS.gene96584.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.67e-58 181
MS.gene96584.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.67e-58 181
MS.gene96584.t1 AT5G11150 33.175 211 134 4 5 213 2 207 7.61e-39 134
MS.gene96584.t1 AT5G11150 33.175 211 134 4 5 213 2 207 1.25e-38 133
MS.gene96584.t1 AT5G22360 31.731 208 138 3 5 210 2 207 1.76e-38 132
MS.gene96584.t1 AT4G32150 34.211 190 122 3 5 193 2 189 1.40e-34 122
MS.gene96584.t1 AT2G25340 32.105 190 126 3 5 193 2 189 4.80e-32 116
MS.gene96584.t1 AT2G33120 67.742 62 20 0 134 195 52 113 4.65e-22 88.2
MS.gene96584.t1 AT3G24890 44.681 94 46 2 113 200 14 107 2.99e-17 75.1

Find 42 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTGAATAAAGAATT+TGG 0.138378 7.3:-46000361 MS.gene96584:CDS
CTTTGCAGGCACAAGATTTA+AGG 0.164312 7.3:-45999667 MS.gene96584:intron
GCAAGTTTGCTAACCTCTTC+TGG 0.229459 7.3:+46000194 None:intergenic
CAATCTTCACTTGACGATTA+AGG 0.242055 7.3:+46000019 None:intergenic
AGCTATTTCAGCAAAGTTTC+CGG 0.255031 7.3:+46000870 None:intergenic
TGCATATGCTCCTTCAATTT+CGG 0.281591 7.3:+46000233 None:intergenic
GGCGCGTGGCACGGTGATTC+TGG 0.320729 7.3:-46000911 MS.gene96584:CDS
TGGTTGCTGGGAGTCTTTGC+AGG 0.323885 7.3:+46000839 None:intergenic
AAAGCTTGAATAAAGAATTT+GGG 0.394970 7.3:-46000360 MS.gene96584:intron
TCCGGTGAAGTTGGTGTGCT+CGG 0.407070 7.3:+46000888 None:intergenic
AACTGTCAACCTTCGTGATC+AGG 0.421098 7.3:-45999980 MS.gene96584:intron
TTTGCAGGCACAAGATTTAA+GGG 0.423640 7.3:-45999666 MS.gene96584:intron
CAAGAAAGGCCATAGCAATA+TGG 0.430285 7.3:+46000446 None:intergenic
ATCACTTGTATACAGCTTTG+TGG 0.457723 7.3:-46000932 MS.gene96584:CDS
TATGTTGGAAAATATTGATC+AGG 0.458760 7.3:-46000135 MS.gene96584:intron
TTCAGAACATGAAGATAAAA+CGG 0.473508 7.3:-45999610 MS.gene96584:CDS
CTCCTTGATGTGATCAAGAA+AGG 0.487927 7.3:+46000432 None:intergenic
AGCTTACTGCCTTGTTGCTG+TGG 0.489435 7.3:-46000482 MS.gene96584:CDS
TCAAGTTCAAGATGTTATGT+TGG 0.490196 7.3:-46000150 MS.gene96584:CDS
AGCAAAGTTTCCGGTGAAGT+TGG 0.500993 7.3:+46000879 None:intergenic
CAGCTTTGTGGCGCGTGGCA+CGG 0.506415 7.3:-46000920 MS.gene96584:CDS
GTAATTTAGACCGAAATTGA+AGG 0.508592 7.3:-46000243 MS.gene96584:intron
GGCAAGTACTGAATCCATCA+TGG 0.518155 7.3:+46000767 None:intergenic
GCCGAGCACACCAACTTCAC+CGG 0.518367 7.3:-46000889 MS.gene96584:CDS
GGCCTTTCTTGATCACATCA+AGG 0.528346 7.3:-46000434 MS.gene96584:CDS
TTCAACTACCTCGCCCATGA+TGG 0.533919 7.3:-46000781 MS.gene96584:CDS
ATCATGGGCGAGGTAGTTGA+AGG 0.542753 7.3:+46000783 None:intergenic
ACTTGCGAAAGTAAAAGCTC+AGG 0.550493 7.3:-46000177 MS.gene96584:CDS
GTATACAGCTTTGTGGCGCG+TGG 0.567820 7.3:-46000925 MS.gene96584:CDS
TACTGAATCCATCATGGGCG+AGG 0.571090 7.3:+46000773 None:intergenic
TTTACCAAAAGATATGGTGG+AGG 0.594903 7.3:-46000406 MS.gene96584:CDS
TGCAGGCACAAGATTTAAGG+GGG 0.606935 7.3:-45999664 MS.gene96584:intron
TTGCAGGCACAAGATTTAAG+GGG 0.610788 7.3:-45999665 MS.gene96584:intron
GGCAATTAAGGAGAAAGATG+TGG 0.621585 7.3:-45999634 MS.gene96584:CDS
CAAAAGACTCCACAGCAACA+AGG 0.623781 7.3:+46000473 None:intergenic
GTAGTTGAAGGTATGACCAT+CGG 0.631667 7.3:+46000795 None:intergenic
GCAAGTACTGAATCCATCAT+GGG 0.632742 7.3:+46000768 None:intergenic
TTGTGTGGAACATCCAGAAG+AGG 0.637264 7.3:-46000207 MS.gene96584:CDS
AAATTCACTTACAACACCGA+TGG 0.653817 7.3:-46000811 MS.gene96584:CDS
CTTTCTTGATCACATCAAGG+AGG 0.668827 7.3:-46000431 MS.gene96584:CDS
GGAGCATATGCAATATTGTG+TGG 0.672159 7.3:-46000222 MS.gene96584:CDS
ATAAAAGGACCTGATCACGA+AGG 0.753478 7.3:+45999971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATGTAATTTAGTTATTTA+TGG + chr7.3:46000725-46000744 None:intergenic 15.0%
!! TAAAAGAAAAAAATAAAACG+AGG + chr7.3:45999753-45999772 None:intergenic 15.0%
!! AAAAGCTTGAATAAAGAATT+TGG - chr7.3:46000135-46000154 MS.gene96584:intron 20.0%
!! AAAGCTTGAATAAAGAATTT+GGG - chr7.3:46000136-46000155 MS.gene96584:CDS 20.0%
!! AGGTATATATATGTCTATAT+TGG - chr7.3:46000381-46000400 MS.gene96584:CDS 20.0%
!! TTATAGTTATGTAACTGATA+TGG - chr7.3:46000795-46000814 MS.gene96584:CDS 20.0%
!! TTTATGGATGAACAAATAAA+AGG + chr7.3:46000543-46000562 None:intergenic 20.0%
!!! TCTTTTACATAATCTAATTG+TGG - chr7.3:45999766-45999785 MS.gene96584:intron 20.0%
!!! TTTTTTTTATTGTATGTGCA+TGG - chr7.3:46000231-46000250 MS.gene96584:CDS 20.0%
! CAAATCATAAATTTCCTTAG+AGG + chr7.3:45999923-45999942 None:intergenic 25.0%
! TATGTTGGAAAATATTGATC+AGG - chr7.3:46000361-46000380 MS.gene96584:CDS 25.0%
! TTCAGAACATGAAGATAAAA+CGG - chr7.3:46000886-46000905 MS.gene96584:CDS 25.0%
!! ATTTTTACACACACATGAAT+AGG - chr7.3:46000679-46000698 MS.gene96584:intron 25.0%
!! TTTTTACACACACATGAATA+GGG - chr7.3:46000680-46000699 MS.gene96584:intron 25.0%
!!! ATTTTATTTGTTTTGTCTGC+AGG - chr7.3:46000453-46000472 MS.gene96584:CDS 25.0%
GTAATTTAGACCGAAATTGA+AGG - chr7.3:46000253-46000272 MS.gene96584:intron 30.0%
TAACTGATATGGTTTGAATG+TGG - chr7.3:46000806-46000825 MS.gene96584:CDS 30.0%
TCAAATGCATTTACATGTAC+TGG - chr7.3:45999872-45999891 MS.gene96584:intron 30.0%
TCAAGTTCAAGATGTTATGT+TGG - chr7.3:46000346-46000365 MS.gene96584:intron 30.0%
! GAGGATTTTACCAAAAGATA+TGG - chr7.3:46000084-46000103 MS.gene96584:intron 30.0%
! GATTTTACCAAAAGATATGG+TGG - chr7.3:46000087-46000106 MS.gene96584:intron 30.0%
! GTTGTAAGTGAATTTTGTGT+TGG + chr7.3:45999680-45999699 None:intergenic 30.0%
! TTTTACACACACATGAATAG+GGG - chr7.3:46000681-46000700 MS.gene96584:intron 30.0%
!! AGTGAAGATTGATGTTTTGA+TGG - chr7.3:46000489-46000508 MS.gene96584:intron 30.0%
!! TGTACTGGTTTCATATATGT+CGG - chr7.3:45999887-45999906 MS.gene96584:intron 30.0%
AAATTCACTTACAACACCGA+TGG - chr7.3:45999685-45999704 MS.gene96584:intron 35.0%
ATCACTTGTATACAGCTTTG+TGG - chr7.3:45999564-45999583 MS.gene96584:CDS 35.0%
CAATCTTCACTTGACGATTA+AGG + chr7.3:46000480-46000499 None:intergenic 35.0%
TAGCTATGACACATGGTTTA+TGG + chr7.3:46000559-46000578 None:intergenic 35.0%
TATGTCTATATTGGATCCTC+TGG - chr7.3:46000390-46000409 MS.gene96584:CDS 35.0%
TCAATGCAATGCGATATATG+AGG - chr7.3:45999798-45999817 MS.gene96584:intron 35.0%
TGCATATGCTCCTTCAATTT+CGG + chr7.3:46000266-46000285 None:intergenic 35.0%
TTTACCAAAAGATATGGTGG+AGG - chr7.3:46000090-46000109 MS.gene96584:intron 35.0%
TTTGCAGGCACAAGATTTAA+GGG - chr7.3:46000830-46000849 MS.gene96584:CDS 35.0%
! AGCTATTTCAGCAAAGTTTC+CGG + chr7.3:45999629-45999648 None:intergenic 35.0%
! CAGTATTATGTTTTCTGCGA+TGG + chr7.3:46000610-46000629 None:intergenic 35.0%
! TTTTCCTCCACCATATCTTT+TGG + chr7.3:46000097-46000116 None:intergenic 35.0%
! TTTTGATCGCCATATTGCTA+TGG - chr7.3:46000041-46000060 MS.gene96584:intron 35.0%
!!! TGAATTTTGTGTTGGTTGCT+GGG + chr7.3:45999672-45999691 None:intergenic 35.0%
AACGATCTGTGCTTCAAAGT+CGG + chr7.3:46000418-46000437 None:intergenic 40.0%
AAGATTTAAGGGGGAATGCA+GGG - chr7.3:46000841-46000860 MS.gene96584:CDS 40.0%
ACTTGCGAAAGTAAAAGCTC+AGG - chr7.3:46000319-46000338 MS.gene96584:intron 40.0%
ATAAAAGGACCTGATCACGA+AGG + chr7.3:46000528-46000547 None:intergenic 40.0%
CAAGAAAGGCCATAGCAATA+TGG + chr7.3:46000053-46000072 None:intergenic 40.0%
CTCCTTGATGTGATCAAGAA+AGG + chr7.3:46000067-46000086 None:intergenic 40.0%
CTTTCTTGATCACATCAAGG+AGG - chr7.3:46000065-46000084 MS.gene96584:intron 40.0%
CTTTGCAGGCACAAGATTTA+AGG - chr7.3:46000829-46000848 MS.gene96584:CDS 40.0%
GGAGCATATGCAATATTGTG+TGG - chr7.3:46000274-46000293 MS.gene96584:intron 40.0%
GGCAATTAAGGAGAAAGATG+TGG - chr7.3:46000862-46000881 MS.gene96584:CDS 40.0%
TCATCACTAGCTATGACACA+TGG + chr7.3:46000566-46000585 None:intergenic 40.0%
TTGCAGGCACAAGATTTAAG+GGG - chr7.3:46000831-46000850 MS.gene96584:CDS 40.0%
! GTAGTTGAAGGTATGACCAT+CGG + chr7.3:45999704-45999723 None:intergenic 40.0%
!! GCAAGTACTGAATCCATCAT+GGG + chr7.3:45999731-45999750 None:intergenic 40.0%
!!! GTGAATTTTGTGTTGGTTGC+TGG + chr7.3:45999673-45999692 None:intergenic 40.0%
AACTGTCAACCTTCGTGATC+AGG - chr7.3:46000516-46000535 MS.gene96584:intron 45.0%
AGCAAAGTTTCCGGTGAAGT+TGG + chr7.3:45999620-45999639 None:intergenic 45.0%
CAAAAGACTCCACAGCAACA+AGG + chr7.3:46000026-46000045 None:intergenic 45.0%
CAAGATTTAAGGGGGAATGC+AGG - chr7.3:46000840-46000859 MS.gene96584:CDS 45.0%
GCAATGCGATATATGAGGCA+CGG - chr7.3:45999803-45999822 MS.gene96584:intron 45.0%
GGCCTTTCTTGATCACATCA+AGG - chr7.3:46000062-46000081 MS.gene96584:intron 45.0%
TCGGACATTGAACACCTCTA+AGG - chr7.3:45999906-45999925 MS.gene96584:intron 45.0%
TGCAGGCACAAGATTTAAGG+GGG - chr7.3:46000832-46000851 MS.gene96584:CDS 45.0%
TTGTGTGGAACATCCAGAAG+AGG - chr7.3:46000289-46000308 MS.gene96584:intron 45.0%
! TGGTTTGAATGTGGCTTTGC+AGG - chr7.3:46000815-46000834 MS.gene96584:CDS 45.0%
!! GCAAGTTTGCTAACCTCTTC+TGG + chr7.3:46000305-46000324 None:intergenic 45.0%
!! GGCAAGTACTGAATCCATCA+TGG + chr7.3:45999732-45999751 None:intergenic 45.0%
ATCATGGGCGAGGTAGTTGA+AGG + chr7.3:45999716-45999735 None:intergenic 50.0%
TACTGAATCCATCATGGGCG+AGG + chr7.3:45999726-45999745 None:intergenic 50.0%
TTCAACTACCTCGCCCATGA+TGG - chr7.3:45999715-45999734 MS.gene96584:intron 50.0%
! AGCTTACTGCCTTGTTGCTG+TGG - chr7.3:46000014-46000033 MS.gene96584:CDS 50.0%
GGGGGAATGCAGGGCAATTA+AGG - chr7.3:46000850-46000869 MS.gene96584:CDS 55.0%
TGGTTGCTGGGAGTCTTTGC+AGG + chr7.3:45999660-45999679 None:intergenic 55.0%
! GTATACAGCTTTGTGGCGCG+TGG - chr7.3:45999571-45999590 MS.gene96584:CDS 55.0%
! TGCTTCAAAGTCGGCTCCAG+AGG + chr7.3:46000409-46000428 None:intergenic 55.0%
!! TCCGGTGAAGTTGGTGTGCT+CGG + chr7.3:45999611-45999630 None:intergenic 55.0%
GCCGAGCACACCAACTTCAC+CGG - chr7.3:45999607-45999626 MS.gene96584:CDS 60.0%
! CAGCTTTGTGGCGCGTGGCA+CGG - chr7.3:45999576-45999595 MS.gene96584:CDS 65.0%
GGCGCGTGGCACGGTGATTC+TGG - chr7.3:45999585-45999604 MS.gene96584:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 45999553 46000965 45999553 ID=MS.gene96584
chr7.3 mRNA 45999553 46000965 45999553 ID=MS.gene96584.t1;Parent=MS.gene96584
chr7.3 exon 46000776 46000965 46000776 ID=MS.gene96584.t1.exon1;Parent=MS.gene96584.t1
chr7.3 CDS 46000776 46000965 46000776 ID=cds.MS.gene96584.t1;Parent=MS.gene96584.t1
chr7.3 exon 46000361 46000502 46000361 ID=MS.gene96584.t1.exon2;Parent=MS.gene96584.t1
chr7.3 CDS 46000361 46000502 46000361 ID=cds.MS.gene96584.t1;Parent=MS.gene96584.t1
chr7.3 exon 46000136 46000256 46000136 ID=MS.gene96584.t1.exon3;Parent=MS.gene96584.t1
chr7.3 CDS 46000136 46000256 46000136 ID=cds.MS.gene96584.t1;Parent=MS.gene96584.t1
chr7.3 exon 45999981 46000043 45999981 ID=MS.gene96584.t1.exon4;Parent=MS.gene96584.t1
chr7.3 CDS 45999981 46000043 45999981 ID=cds.MS.gene96584.t1;Parent=MS.gene96584.t1
chr7.3 exon 45999553 45999681 45999553 ID=MS.gene96584.t1.exon5;Parent=MS.gene96584.t1
chr7.3 CDS 45999553 45999681 45999553 ID=cds.MS.gene96584.t1;Parent=MS.gene96584.t1
Gene Sequence

>MS.gene96584

ATGGCGCAACAATCACTTGTATACAGCTTTGTGGCGCGTGGCACGGTGATTCTGGCCGAGCACACCAACTTCACCGGAAACTTTGCTGAAATAGCTTTGCAATGCCTGCAAAGACTCCCAGCAACCAACACAAAATTCACTTACAACACCGATGGTCATACCTTCAACTACCTCGCCCATGATGGATTCAGTACTTGCCTCGTTTTATTTTTTTCTTTTACATAATCTAATTGTGGAATAGCATATCAATGCAATGCGATATATGAGGCACGGACACTAGTGTTTAGATCTTGATAATAATTTAAGAAAATATAATTGATCAAATGCATTTACATGTACTGGTTTCATATATGTCGGACATTGAACACCTCTAAGGAAATTTATGATTTGATTTATATATTGTTTTCTTTTTCTTTCTAAAATTCTACTATATTATTAGACTTACATGATCTATTATCTTTAGCTTACTGCCTTGTTGCTGTGGAGTCTTTTGATCGCCATATTGCTATGGCCTTTCTTGATCACATCAAGGAGGATTTTACCAAAAGATATGGTGGAGGAAAAGCTGCAACAGCAACATCTAAAAGCTTGAATAAAGAATTTGGGTACTCAAGTTTATAACAATTTTGTGTATAGAAAATGTGTCACAAAAGTTGAATTGCATTAATTAACATGAAGTTTTTTTTATTGTATGTGCATGGTAATTTAGACCGAAATTGAAGGAGCATATGCAATATTGTGTGGAACATCCAGAAGAGGTTAGCAAACTTGCGAAAGTAAAAGCTCAGGTTTCTCAAGTTCAAGATGTTATGTTGGAAAATATTGATCAGGTATATATATGTCTATATTGGATCCTCTGGAGCCGACTTTGAAGCACAGATCGTTAGATTAAGAGAGAAAATTTTATTTGTTTTGTCTGCAGGTCCTTAATCGTCAAGTGAAGATTGATGTTTTGATGGACAAAACTGTCAACCTTCGTGATCAGGTCCTTTTATTTGTTCATCCATAAACCATGTGTCATAGCTAGTGATGAAATTTTCACAAAATCACAAGACCATCGCAGAAAACATAATACTGTGTTTATTTATGACTGTTCTAGTTCATGAATTACTTACTATATATTTATATTTTTACACACACATGAATAGGGGTCAAGTATAATCTAGTAACCATAAATAACTAAATTACATTGTCAAATTATCTTAACAGATATATACACATAATTTTAAATAAATTAGACATTTATAGTTATGTAACTGATATGGTTTGAATGTGGCTTTGCAGGCACAAGATTTAAGGGGGAATGCAGGGCAATTAAGGAGAAAGATGTGGTTTCAGAACATGAAGATAAAACGGATAGTTCTTGCTATCATAATTGTCATCATTCTAATAATTGTTCTTGTTCTTACATAG

Protein sequence

>MS.gene96584.t1

MAQQSLVYSFVARGTVILAEHTNFTGNFAEIALQCLQRLPATNTKFTYNTDGHTFNYLAHDGFTYCLVAVESFDRHIAMAFLDHIKEDFTKRYGGGKAATATSKSLNKEFGPKLKEHMQYCVEHPEEVSKLAKVKAQVSQVQDVMLENIDQVLNRQVKIDVLMDKTVNLRDQAQDLRGNAGQLRRKMWFQNMKIKRIVLAIIIVIILIIVLVLT