AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99234

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99234.t1 MTR_1g040195 83.442 459 53 1 1 459 1 436 0.0 788
MS.gene99234.t1 MTR_1g040215 74.837 461 114 2 1 459 1 461 0.0 716
MS.gene99234.t1 MTR_1g040175 73.970 461 115 3 1 459 1 458 0.0 700
MS.gene99234.t1 MTR_1g040320 69.783 460 103 2 1 459 1 425 0.0 648
MS.gene99234.t1 MTR_1g040315 70.435 460 92 4 1 459 1 417 0.0 644
MS.gene99234.t1 MTR_7g114550 58.371 442 176 5 2 438 3 441 0.0 545
MS.gene99234.t1 MTR_7g114560 58.242 455 181 6 5 454 6 456 0.0 544
MS.gene99234.t1 MTR_5g076700 57.709 454 186 4 5 454 6 457 0.0 543
MS.gene99234.t1 MTR_7g114470 57.582 455 186 4 5 454 7 459 0.0 538
MS.gene99234.t1 MTR_1g081660 59.817 438 171 5 4 438 13 448 0.0 530
MS.gene99234.t1 MTR_7g114520 56.798 456 188 6 5 454 7 459 0.0 530
MS.gene99234.t1 MTR_7g114490 59.165 431 168 5 5 430 6 433 0.0 530
MS.gene99234.t1 MTR_0042s0190 67.340 297 95 2 165 459 1 297 2.26e-143 412
MS.gene99234.t1 MTR_7g114510 52.188 320 133 6 146 454 7 317 3.51e-108 323
MS.gene99234.t1 MTR_0042s0190 67.241 232 74 2 230 459 1 232 5.48e-108 319
MS.gene99234.t1 MTR_0502s0010 58.264 242 91 5 100 335 1 238 1.34e-95 288
MS.gene99234.t1 MTR_7g114420 46.939 294 123 6 165 454 1 265 1.01e-84 261
MS.gene99234.t1 MTR_0042s0230 80.272 147 29 0 1 147 1 147 1.91e-82 251
MS.gene99234.t1 MTR_8g039950 90.698 129 12 0 1 129 1 129 1.53e-79 243
MS.gene99234.t1 MTR_3g098980 32.710 428 269 8 9 430 14 428 1.79e-78 251
MS.gene99234.t1 MTR_3g035000 54.132 242 79 5 195 429 39 255 9.94e-77 242
MS.gene99234.t1 MTR_8g096530 34.257 397 247 7 1 391 1 389 1.23e-75 244
MS.gene99234.t1 MTR_7g027175 34.109 387 241 7 12 391 19 398 4.94e-72 234
MS.gene99234.t1 MTR_7g114500 60.667 150 59 0 2 151 3 152 5.10e-63 201
MS.gene99234.t1 MTR_3g112180 33.760 391 235 10 17 394 20 399 6.32e-57 195
MS.gene99234.t1 MTR_3g051790 59.589 146 59 0 2 147 6 151 6.28e-56 184
MS.gene99234.t1 MTR_1g053315 31.377 443 278 15 17 438 26 463 1.73e-55 191
MS.gene99234.t1 MTR_4g055170 29.186 442 288 10 16 437 19 455 4.59e-54 187
MS.gene99234.t1 MTR_3g071320 30.115 435 273 12 17 433 13 434 2.20e-50 177
MS.gene99234.t1 MTR_4g007540 29.241 448 273 16 5 430 1 426 9.29e-48 170
MS.gene99234.t1 MTR_2g105330 28.764 445 278 13 5 430 1 425 5.26e-47 168
MS.gene99234.t1 MTR_8g075610 30.536 429 273 14 17 434 2 416 1.78e-46 166
MS.gene99234.t1 MTR_2g064495 28.539 445 279 13 5 430 1 425 9.52e-46 164
MS.gene99234.t1 MTR_7g110610 27.293 447 247 14 5 438 1 382 1.05e-43 158
MS.gene99234.t1 MTR_2g015430 29.639 415 277 10 30 434 25 434 3.95e-41 152
MS.gene99234.t1 MTR_7g085930 28.842 475 262 17 9 432 6 455 1.14e-40 152
MS.gene99234.t1 MTR_7g084940 27.273 385 257 9 17 389 24 397 2.43e-40 150
MS.gene99234.t1 MTR_7g085990 28.406 433 242 13 9 390 2 417 1.30e-37 143
MS.gene99234.t1 MTR_6g053810 29.412 408 252 15 57 437 64 462 6.48e-37 141
MS.gene99234.t1 MTR_7g084910 26.733 404 251 12 1 389 11 384 1.01e-36 140
MS.gene99234.t1 MTR_4g127650 27.434 452 277 16 17 434 18 452 1.23e-36 140
MS.gene99234.t1 MTR_3g026950 25.728 412 264 11 13 390 6 409 8.42e-36 138
MS.gene99234.t1 MTR_3g465930 27.212 452 292 15 16 437 17 461 3.51e-35 136
MS.gene99234.t1 MTR_4g097090 27.373 453 279 16 16 434 16 452 1.24e-34 135
MS.gene99234.t1 MTR_3g465990 26.754 456 285 16 16 437 17 457 3.02e-34 134
MS.gene99234.t1 MTR_7g086000 27.250 400 238 17 61 422 57 441 1.47e-32 129
MS.gene99234.t1 MTR_3g465980 26.395 466 274 16 16 437 17 457 2.81e-32 128
MS.gene99234.t1 MTR_4g013050 25.227 440 264 14 12 430 7 402 3.61e-32 127
MS.gene99234.t1 MTR_3g465910 26.258 457 293 15 16 440 17 461 4.32e-32 129
MS.gene99234.t1 MTR_7g056087 26.471 442 279 15 17 432 12 433 1.03e-30 123
MS.gene99234.t1 MTR_4g088765 72.603 73 20 0 1 73 1 73 4.53e-30 113
MS.gene99234.t1 MTR_3g465880 27.412 456 289 17 16 437 17 464 3.70e-29 119
MS.gene99234.t1 MTR_3g049150 24.818 411 273 12 6 388 1 403 1.56e-26 112
MS.gene99234.t1 MTR_3g465950 26.608 342 221 9 16 336 17 349 2.29e-25 108
MS.gene99234.t1 MTR_5g090830 25.000 460 272 17 7 425 8 435 6.81e-25 107
MS.gene99234.t1 MTR_5g090810 24.783 460 273 17 7 425 8 435 3.98e-24 105
MS.gene99234.t1 MTR_3g450690 25.467 428 238 14 64 448 72 461 1.49e-23 103
MS.gene99234.t1 MTR_5g084870 21.670 443 297 15 15 437 9 421 1.54e-23 102
MS.gene99234.t1 MTR_5g090880 24.750 400 240 14 61 425 64 437 1.87e-23 103
MS.gene99234.t1 MTR_3g101960 28.840 319 202 12 23 327 30 337 2.82e-23 102
MS.gene99234.t1 MTR_3g101930 28.771 358 209 15 9 334 7 350 2.91e-23 102
MS.gene99234.t1 MTR_3g450710 26.065 399 225 15 64 423 65 432 5.62e-23 101
MS.gene99234.t1 MTR_4g094835 24.821 419 245 18 46 428 49 433 1.60e-21 97.1
MS.gene99234.t1 MTR_0190s0020 26.651 439 250 19 23 428 30 429 1.85e-21 96.7
MS.gene99234.t1 MTR_4g022290 24.752 404 252 13 23 401 31 407 2.17e-21 96.7
MS.gene99234.t1 MTR_5g084850 22.308 390 263 13 15 386 9 376 3.09e-21 96.3
MS.gene99234.t1 MTR_4g094838 23.864 440 272 15 23 438 29 429 3.79e-21 95.9
MS.gene99234.t1 MTR_4g022800 26.147 436 261 20 23 428 25 429 4.56e-21 95.5
MS.gene99234.t1 MTR_8g041770 24.138 435 282 14 14 428 22 428 7.33e-21 95.1
MS.gene99234.t1 MTR_3g102020 27.168 346 198 14 16 327 13 338 7.71e-21 95.1
MS.gene99234.t1 MTR_3g101900 24.773 440 252 18 9 399 7 416 1.06e-20 94.7
MS.gene99234.t1 MTR_4g094842 24.449 454 289 19 5 428 6 435 1.13e-20 94.4
MS.gene99234.t1 MTR_8g079950 21.368 468 318 13 15 459 9 449 1.69e-20 94.0
MS.gene99234.t1 MTR_4g094832 25.745 369 224 14 94 438 85 427 3.10e-20 93.2
MS.gene99234.t1 MTR_3g101970 28.025 314 198 12 23 327 30 324 6.14e-20 92.4
MS.gene99234.t1 MTR_8g079970 22.959 392 265 14 15 386 9 383 6.39e-20 92.0
MS.gene99234.t1 MTR_3g088420 24.832 447 258 19 23 435 25 427 1.37e-19 91.3
MS.gene99234.t1 MTR_3g102010 25.542 415 243 15 24 399 32 419 1.56e-19 91.3
MS.gene99234.t1 MTR_7g026860 25.701 428 269 17 23 428 25 425 1.72e-19 90.9
MS.gene99234.t1 MTR_5g026320 25.815 399 254 13 22 395 33 414 4.61e-19 89.7
MS.gene99234.t1 MTR_3g465870 27.817 284 181 10 16 282 19 295 8.42e-19 88.6
MS.gene99234.t1 MTR_4g022720 24.533 428 271 16 23 428 25 422 9.72e-19 88.6
MS.gene99234.t1 MTR_4g022670 25.747 435 263 19 23 428 25 428 1.46e-18 88.2
MS.gene99234.t1 MTR_4g022825 25.450 444 252 20 23 428 25 427 8.93e-17 82.8
MS.gene99234.t1 MTR_8g467590 23.308 399 259 20 15 386 9 387 2.48e-16 81.3
MS.gene99234.t1 MTR_5g084900 21.978 364 229 13 64 417 9 327 4.01e-16 80.1
MS.gene99234.t1 MTR_7g114417 56.364 55 23 1 89 142 29 83 2.03e-13 65.9
MS.gene99234.t1 MTR_7g114417 65.000 40 14 0 2 41 3 42 9.59e-11 58.5
MS.gene99234.t1 MTR_3g051820 49.333 75 36 2 365 438 1 74 1.96e-12 63.5
MS.gene99234.t1 MTR_3g049140 27.742 155 94 4 250 388 48 200 6.28e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99234.t1 AT5G17540 57.511 466 186 9 1 459 1 461 0.0 518
MS.gene99234.t1 AT3G03480 57.143 448 175 12 5 444 15 453 5.18e-168 481
MS.gene99234.t1 AT5G63560 33.409 443 269 11 1 435 1 425 8.60e-78 249
MS.gene99234.t1 AT5G41040 31.881 436 273 10 9 434 33 454 3.56e-76 246
MS.gene99234.t1 AT5G41040 31.881 436 273 10 9 434 17 438 4.59e-76 245
MS.gene99234.t1 AT3G48720 30.963 436 283 10 1 430 1 424 8.72e-74 238
MS.gene99234.t1 AT3G62160 31.905 420 253 12 5 411 1 400 7.46e-55 189
MS.gene99234.t1 AT1G27620 28.767 438 283 11 10 435 10 430 1.48e-54 188
MS.gene99234.t1 AT2G40230 33.333 387 234 11 17 388 13 390 2.80e-50 177
MS.gene99234.t1 AT1G28680 28.700 446 297 13 16 445 11 451 8.64e-48 171
MS.gene99234.t1 AT2G19070 28.966 435 258 13 35 434 29 447 1.18e-47 170
MS.gene99234.t1 AT1G65450 28.125 448 286 13 1 430 12 441 4.39e-45 163
MS.gene99234.t1 AT1G03390 30.317 442 285 13 7 434 23 455 7.04e-45 163
MS.gene99234.t1 AT5G48930 28.506 435 279 12 17 434 11 430 1.61e-44 161
MS.gene99234.t1 AT3G47170 28.243 478 276 19 7 438 10 466 4.63e-44 161
MS.gene99234.t1 AT5G57840 27.376 442 275 11 17 432 11 432 5.68e-44 160
MS.gene99234.t1 AT5G07080 29.274 468 263 18 7 437 12 448 1.27e-43 159
MS.gene99234.t1 AT1G28680 31.395 344 222 10 16 349 11 350 4.65e-43 156
MS.gene99234.t1 AT2G25150 29.596 446 283 17 15 439 19 454 4.88e-37 141
MS.gene99234.t1 AT2G23510 27.897 466 287 17 1 437 5 450 5.58e-36 138
MS.gene99234.t1 AT1G78990 27.735 393 245 13 62 433 65 439 1.01e-31 126
MS.gene99234.t1 AT1G31490 25.428 409 253 14 6 387 3 386 1.45e-29 120
MS.gene99234.t1 AT1G32910 26.425 386 232 15 62 423 63 420 9.71e-26 109
MS.gene99234.t1 AT5G07860 25.054 459 288 14 4 435 12 441 6.75e-25 107
MS.gene99234.t1 AT3G50270 26.667 420 237 17 23 401 30 419 2.54e-24 105
MS.gene99234.t1 AT3G26040 23.618 398 257 15 15 388 9 383 9.95e-24 103
MS.gene99234.t1 AT4G15390 24.183 459 285 17 3 430 2 428 1.66e-23 102
MS.gene99234.t1 AT4G15390 24.183 459 285 17 3 430 2 428 1.66e-23 102
MS.gene99234.t1 AT1G65450 26.599 297 189 8 158 438 2 285 7.52e-23 98.6
MS.gene99234.t1 AT5G07870 26.277 411 257 15 64 446 72 464 7.83e-23 101
MS.gene99234.t1 AT5G07850 27.335 439 247 19 4 399 12 421 1.46e-22 100
MS.gene99234.t1 AT4G31910 26.829 410 267 13 48 430 48 451 2.38e-22 99.8
MS.gene99234.t1 AT5G42830 26.366 421 248 14 15 399 23 417 5.61e-22 98.6
MS.gene99234.t1 AT5G38130 25.313 399 251 14 23 396 48 424 6.49e-22 98.6
MS.gene99234.t1 AT3G50280 26.515 396 250 16 23 395 28 405 8.02e-22 97.8
MS.gene99234.t1 AT1G24430 24.569 464 276 19 12 445 8 427 1.02e-21 97.4
MS.gene99234.t1 AT1G24420 26.933 401 233 18 15 386 11 380 3.44e-21 95.9
MS.gene99234.t1 AT1G24420 26.933 401 233 18 15 386 11 380 3.44e-21 95.9
MS.gene99234.t1 AT5G16410 26.074 349 221 13 62 388 89 422 7.28e-21 95.5
MS.gene99234.t1 AT3G50300 26.966 356 221 15 64 392 59 402 4.32e-20 92.8
MS.gene99234.t1 AT5G23970 25.670 448 273 19 13 438 7 416 7.57e-19 89.0
MS.gene99234.t1 AT5G67160 25.428 409 242 15 58 435 50 426 1.65e-18 87.8
MS.gene99234.t1 AT5G47950 23.767 446 267 18 9 427 6 405 6.41e-15 77.0
MS.gene99234.t1 AT5G23940 23.581 458 254 20 41 438 28 449 4.08e-14 74.7
MS.gene99234.t1 AT3G50280 24.907 269 184 10 136 391 8 271 2.82e-13 70.9
MS.gene99234.t1 AT3G29635 23.133 415 278 17 46 431 45 447 2.11e-12 69.3
MS.gene99234.t1 AT3G50290 25.000 224 134 8 138 342 16 224 9.83e-12 65.9
MS.gene99234.t1 AT3G23840 26.872 227 137 10 80 296 81 288 8.17e-11 64.3

Find 96 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTGATGATTTCGAATT+TGG 0.114001 1.2:-24765149 None:intergenic
GGGTCTTTCCCTTCCATATT+TGG 0.169165 1.2:-24762619 None:intergenic
ATCCATTTGCAGGTAGATTA+AGG 0.198110 1.2:+24762689 MS.gene99234:CDS
TTTGATGGTCTTACAAATTC+AGG 0.203685 1.2:-24762502 None:intergenic
GAATTAATGATAGCATATTT+TGG 0.210015 1.2:-24765727 None:intergenic
GTATTCAGTTGCGGATTTAA+TGG 0.220815 1.2:+24765408 MS.gene99234:CDS
TTTGAAAACGTAACCCTTCT+TGG 0.271683 1.2:-24762567 None:intergenic
CGAACTTCCTCGTCCGTATC+TGG 0.272136 1.2:-24765205 None:intergenic
GGACTTAGAGATGTTGATTT+TGG 0.272907 1.2:+24765496 MS.gene99234:CDS
CACCCAAGGCACTCATGAAT+TGG 0.305268 1.2:-24764895 None:intergenic
CATTTCATCTAAGGGTATAA+TGG 0.312537 1.2:-24765047 None:intergenic
TTATAAATATGATCCAAATA+TGG 0.321793 1.2:+24762606 MS.gene99234:CDS
TTGGATCATCTTTAGTAGAT+TGG 0.332279 1.2:-24765708 None:intergenic
AAACTAATTGAAAATCCATT+AGG 0.336479 1.2:+24765331 MS.gene99234:CDS
GATGTTAGTTTGAAAGAATT+TGG 0.347283 1.2:+24762781 MS.gene99234:CDS
GCGTTTCCATAGTAACCTTT+TGG 0.359409 1.2:-24765274 None:intergenic
CCTGCAGTTGTAATTGCAAC+AGG 0.365928 1.2:-24765307 None:intergenic
AACTCAAGTGCATACCCTAA+TGG 0.368393 1.2:-24765346 None:intergenic
TGCTAAAGGAGAGGAAGGTT+TGG 0.383515 1.2:+24765606 MS.gene99234:CDS
AACTAATTGAAAATCCATTA+GGG 0.389241 1.2:+24765332 MS.gene99234:CDS
CCGTTGCCAAAAGGTTACTA+TGG 0.390644 1.2:+24765268 MS.gene99234:CDS
CGAATTTGGATTGTTGTTGT+AGG 0.393458 1.2:-24765135 None:intergenic
TCACTGTGAATAAAAGAGAT+TGG 0.393886 1.2:-24762468 None:intergenic
CATTATACCCTTAGATGAAA+TGG 0.396592 1.2:+24765048 MS.gene99234:CDS
GCGGTGGACCATTTCATCTA+AGG 0.404967 1.2:-24765056 None:intergenic
GTCCAATTCATGAGTGCCTT+GGG 0.406998 1.2:+24764893 MS.gene99234:CDS
TCCATTTGCAGGTAGATTAA+GGG 0.408450 1.2:+24762690 MS.gene99234:CDS
CCTTGGGTGAAATTTCTTGC+GGG 0.426188 1.2:+24764909 MS.gene99234:CDS
TGCGGATTTAATGGTTATCA+AGG 0.430701 1.2:+24765417 MS.gene99234:CDS
AAATCATCACTGCATGCCTT+TGG 0.430886 1.2:+24765158 MS.gene99234:CDS
GATGAGCGATGCGATGGGTT+TGG 0.433400 1.2:+24764871 MS.gene99234:CDS
AGGTCCTGCTAGGAAACTTA+TGG 0.433584 1.2:+24762714 MS.gene99234:CDS
CCTGTTGCAATTACAACTGC+AGG 0.441218 1.2:+24765307 MS.gene99234:CDS
TATTTATAAAATTGTATCAC+TGG 0.450663 1.2:-24762592 None:intergenic
CACTCCAATCCTTCTTATCC+AGG 0.451699 1.2:+24762879 MS.gene99234:CDS
TCCCTTAATCTACCTGCAAA+TGG 0.452547 1.2:-24762691 None:intergenic
GGTCCAATTCATGAGTGCCT+TGG 0.454709 1.2:+24764892 MS.gene99234:CDS
CACTGTGAATAAAAGAGATT+GGG 0.457495 1.2:-24762467 None:intergenic
CCATACGATGAGCGATGCGA+TGG 0.473629 1.2:+24764865 MS.gene99234:CDS
AATAATATACCTGGATAAGA+AGG 0.480629 1.2:-24762888 None:intergenic
CCATCGCATCGCTCATCGTA+TGG 0.492481 1.2:-24764865 None:intergenic
TTGATGGTCTTACAAATTCA+GGG 0.497631 1.2:-24762501 None:intergenic
AAACTTATGGTGGATTGCAC+TGG 0.500723 1.2:+24762727 MS.gene99234:CDS
AACTCGTCCAAACAAGGAAA+AGG 0.509960 1.2:-24762820 None:intergenic
CGATGAGTGCATGTTACTCT+TGG 0.517477 1.2:-24764992 None:intergenic
TATACCTGGATAAGAAGGAT+TGG 0.520763 1.2:-24762883 None:intergenic
TTGTTTGCCAACTCAAGCCA+TGG 0.521796 1.2:+24765639 MS.gene99234:CDS
GTATATGGAGGACCAGCTAC+AGG 0.522562 1.2:+24765532 MS.gene99234:CDS
GCCTTGGGTGAAATTTCTTG+CGG 0.522636 1.2:+24764908 MS.gene99234:CDS
TGCCATACCGGTGAGATTGA+AGG 0.523692 1.2:-24764941 None:intergenic
GAGCAAGTTCCTGACACAAA+AGG 0.530837 1.2:+24765022 MS.gene99234:CDS
ACACCAACTCCTCCTGTAGC+TGG 0.532688 1.2:-24765544 None:intergenic
CTGGTTGTAATGCGATTGTA+CGG 0.537038 1.2:-24765186 None:intergenic
AAATATGATCCAAATATGGA+AGG 0.544870 1.2:+24762610 MS.gene99234:CDS
GTGTCTGATGTTACACATGC+TGG 0.545058 1.2:+24765475 MS.gene99234:CDS
CGGTGGACCATTTCATCTAA+GGG 0.545372 1.2:-24765055 None:intergenic
TGAGTGCATGTTACTCTTGG+TGG 0.546616 1.2:-24764989 None:intergenic
AACCTCTCCATGGCTTGAGT+TGG 0.550792 1.2:-24765646 None:intergenic
AATATGATCCAAATATGGAA+GGG 0.552554 1.2:+24762611 MS.gene99234:CDS
ATAGAGAGCTTCTAAACGCA+AGG 0.554177 1.2:+24764963 MS.gene99234:CDS
CAGATATAGATGACCAAGAA+GGG 0.559168 1.2:+24762554 MS.gene99234:CDS
AACCTTCAATCTCACCGGTA+TGG 0.559901 1.2:+24764939 MS.gene99234:CDS
CTCTTTAACGAACCTCTCCA+TGG 0.560979 1.2:-24765656 None:intergenic
CGATTGTACGGCAACGCCAA+AGG 0.564878 1.2:-24765174 None:intergenic
TCAGATATAGATGACCAAGA+AGG 0.565827 1.2:+24762553 MS.gene99234:CDS
AGAATACATGTATTCAGTTG+CGG 0.568895 1.2:+24765399 MS.gene99234:CDS
GGACCAGCTACAGGAGGAGT+TGG 0.569672 1.2:+24765541 MS.gene99234:CDS
TCCACCATAAGTTTCCTAGC+AGG 0.571714 1.2:-24762718 None:intergenic
CGAAGTGCAGATAACTCGGT+TGG 0.572288 1.2:-24765094 None:intergenic
AGAACGAAGTGCAGATAACT+CGG 0.585268 1.2:-24765098 None:intergenic
AATCGCATTACAACCAGATA+CGG 0.585517 1.2:+24765192 MS.gene99234:CDS
GCGGATTTAATGGTTATCAA+GGG 0.587368 1.2:+24765418 MS.gene99234:CDS
AGAAGCTCTCTATGCCATAC+CGG 0.587394 1.2:-24764953 None:intergenic
GAATGAACCTTCAATCTCAC+CGG 0.587572 1.2:+24764934 MS.gene99234:CDS
GATTAAGGGAAGGTCCTGCT+AGG 0.590882 1.2:+24762704 MS.gene99234:CDS
TGCCAACTCAAGCCATGGAG+AGG 0.591210 1.2:+24765644 MS.gene99234:CDS
AAAAGAAACTCGTCCAAACA+AGG 0.593076 1.2:-24762826 None:intergenic
ATATAAAAGCTAGCAACACC+AGG 0.611199 1.2:-24765571 None:intergenic
CATACGATGAGCGATGCGAT+GGG 0.611919 1.2:+24764866 MS.gene99234:CDS
CCCGCAAGAAATTTCACCCA+AGG 0.612458 1.2:-24764909 None:intergenic
TGATGGTCTTACAAATTCAG+GGG 0.623986 1.2:-24762500 None:intergenic
GGAGGAGTTGGTGTTACACC+TGG 0.626042 1.2:+24765553 MS.gene99234:CDS
GCTTGAGTTGGCAAACAAAG+TGG 0.628285 1.2:-24765634 None:intergenic
TCCTGCTAGGAAACTTATGG+TGG 0.635781 1.2:+24762717 MS.gene99234:CDS
TCGTCCAAACAAGGAAAAGG+AGG 0.640474 1.2:-24762817 None:intergenic
ATTCTCACCACGGTAAAATG+TGG 0.643343 1.2:-24765445 None:intergenic
ATGGTGGATTGCACTGGAGA+AGG 0.645652 1.2:+24762733 MS.gene99234:CDS
GATTGTTGTTGTAGGTGACG+TGG 0.649549 1.2:-24765127 None:intergenic
TAGAGAGCTTCTAAACGCAA+GGG 0.655440 1.2:+24764964 MS.gene99234:CDS
TTTGCAGGTAGATTAAGGGA+AGG 0.667024 1.2:+24762694 MS.gene99234:CDS
TATGGAGGACCAGCTACAGG+AGG 0.683939 1.2:+24765535 MS.gene99234:CDS
ATTACAACCAGATACGGACG+AGG 0.685001 1.2:+24765198 MS.gene99234:CDS
TTAATCACATCTGAATAACC+AGG 0.690578 1.2:-24762856 None:intergenic
GTAACACGTCTCAAGTGTGG+TGG 0.692143 1.2:+24764818 MS.gene99234:CDS
CAGGTAACACGTCTCAAGTG+TGG 0.695040 1.2:+24764815 MS.gene99234:intron
CACTAAGTACATTCTCACCA+CGG 0.698784 1.2:-24765455 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAAAAATTAATATT+CGG - chr1.2:24763465-24763484 None:intergenic 0.0%
!! ACTAGTTATTTAATTAAAAA+AGG + chr1.2:24763163-24763182 MS.gene99234:intron 10.0%
!! ATCAAAATAAAATTATGATT+AGG + chr1.2:24764425-24764444 MS.gene99234:intron 10.0%
!! TATATAATATATGTAAAGTT+CGG - chr1.2:24763015-24763034 None:intergenic 10.0%
!!! CAAAATTGAATTTTAAATTA+AGG + chr1.2:24764196-24764215 MS.gene99234:intron 10.0%
!! AAAACTCAAAATACAAAATA+TGG - chr1.2:24763850-24763869 None:intergenic 15.0%
!! ATATAATATATGTAAAGTTC+GGG - chr1.2:24763014-24763033 None:intergenic 15.0%
!! TAGTTATTCAAATTTATTAC+TGG + chr1.2:24763307-24763326 MS.gene99234:intron 15.0%
!! TATATATATATAGAAACAAG+TGG + chr1.2:24764160-24764179 MS.gene99234:intron 15.0%
!! TATTTATAAAATTGTATCAC+TGG - chr1.2:24762595-24762614 None:intergenic 15.0%
!! TTATAAATATGATCCAAATA+TGG + chr1.2:24762606-24762625 MS.gene99234:CDS 15.0%
!! TTGAATATAATTACTTCAAA+TGG - chr1.2:24764008-24764027 None:intergenic 15.0%
!!! AAGATTTGAGTTAATTAATT+AGG - chr1.2:24764355-24764374 None:intergenic 15.0%
!!! TAAGTTTGCTTATATTTTAT+TGG - chr1.2:24763119-24763138 None:intergenic 15.0%
!!! TTAAAATTTAAGATACGATA+CGG - chr1.2:24763914-24763933 None:intergenic 15.0%
!! AAACTAATTGAAAATCCATT+AGG + chr1.2:24765331-24765350 MS.gene99234:CDS 20.0%
!! AAACTCAAAAGCAAAAAAAA+TGG - chr1.2:24763823-24763842 None:intergenic 20.0%
!! AACTAATTGAAAATCCATTA+GGG + chr1.2:24765332-24765351 MS.gene99234:CDS 20.0%
!! AATCACAAGTGTTTAATTAT+GGG + chr1.2:24764383-24764402 MS.gene99234:intron 20.0%
!! ATGATTAGGAAAATACTAAA+TGG + chr1.2:24764439-24764458 MS.gene99234:intron 20.0%
!! GAAAATATCAATAATATACC+TGG - chr1.2:24762900-24762919 None:intergenic 20.0%
!! TATAAATGGATAGCATTTAA+AGG + chr1.2:24763263-24763282 MS.gene99234:intron 20.0%
!! TATAATATATGTAAAGTTCG+GGG - chr1.2:24763013-24763032 None:intergenic 20.0%
!! TTCTTGTAAAAGAATAAATC+AGG - chr1.2:24764552-24764571 None:intergenic 20.0%
!!! AGTCTTTGATTTTCTTTTTA+TGG + chr1.2:24764693-24764712 MS.gene99234:intron 20.0%
!!! ATATTTTCATGATGTATCAA+AGG + chr1.2:24762912-24762931 MS.gene99234:intron 20.0%
!!! CTTTTCTAATAATATCAACA+GGG - chr1.2:24762642-24762661 None:intergenic 20.0%
!!! GAATTAATGATAGCATATTT+TGG - chr1.2:24765730-24765749 None:intergenic 20.0%
!!! GTCTTTGATTTTCTTTTTAT+GGG + chr1.2:24764694-24764713 MS.gene99234:intron 20.0%
!!! TAATTATATTCAAAGTGTCT+TGG + chr1.2:24764015-24764034 MS.gene99234:intron 20.0%
!!! TGCTTATATTTTATTGGAAA+GGG - chr1.2:24763113-24763132 None:intergenic 20.0%
!!! TTGCTTATATTTTATTGGAA+AGG - chr1.2:24763114-24763133 None:intergenic 20.0%
! AAATATGATCCAAATATGGA+AGG + chr1.2:24762610-24762629 MS.gene99234:CDS 25.0%
! AATAATATACCTGGATAAGA+AGG - chr1.2:24762891-24762910 None:intergenic 25.0%
! AATATGATCCAAATATGGAA+GGG + chr1.2:24762611-24762630 MS.gene99234:CDS 25.0%
! ACAGACATAGAAAAACAATA+AGG - chr1.2:24763783-24763802 None:intergenic 25.0%
! ATACCTTTCAAAAATGCTAA+AGG + chr1.2:24765592-24765611 MS.gene99234:CDS 25.0%
! ATCACAAGTGTTTAATTATG+GGG + chr1.2:24764384-24764403 MS.gene99234:intron 25.0%
! CAATCACAAGTGTTTAATTA+TGG + chr1.2:24764382-24764401 MS.gene99234:intron 25.0%
! CCAAATATAATAGAAACATC+AGG - chr1.2:24763563-24763582 None:intergenic 25.0%
! TATCAAACAAGACAAAACAT+CGG - chr1.2:24764621-24764640 None:intergenic 25.0%
! TCGAATAGAATTGCTATAAA+TGG + chr1.2:24763249-24763268 MS.gene99234:intron 25.0%
! TCGATATTCAAACATTAACT+CGG - chr1.2:24763233-24763252 None:intergenic 25.0%
! TGTAAGAGATGTGATATATA+TGG - chr1.2:24764774-24764793 None:intergenic 25.0%
!! CAGATGTATTTGAAAAGTAT+CGG - chr1.2:24763497-24763516 None:intergenic 25.0%
!! CCTGATGTTTCTATTATATT+TGG + chr1.2:24763560-24763579 MS.gene99234:intron 25.0%
!! GATACTTTTCAAATACATCT+GGG + chr1.2:24763496-24763515 MS.gene99234:intron 25.0%
!! GATGTTAGTTTGAAAGAATT+TGG + chr1.2:24762781-24762800 MS.gene99234:CDS 25.0%
!! GCTTTTCTAATAATATCAAC+AGG - chr1.2:24762643-24762662 None:intergenic 25.0%
!!! AATTTTATTTCATGAGGTGT+TGG - chr1.2:24762529-24762548 None:intergenic 25.0%
!!! ATTGTTTTTCTATGTCTGTT+TGG + chr1.2:24763784-24763803 MS.gene99234:intron 25.0%
!!! CTATTGTTTTATTGTGTGAA+GGG + chr1.2:24763742-24763761 MS.gene99234:intron 25.0%
!!! GAGAGTAATTTTATTTCATG+AGG - chr1.2:24762535-24762554 None:intergenic 25.0%
!!! TCAATCATTTTGAAGTATCT+AGG - chr1.2:24763373-24763392 None:intergenic 25.0%
ACCAACAATCCAATACTTAA+TGG + chr1.2:24764640-24764659 MS.gene99234:intron 30.0%
AGAATACATGTATTCAGTTG+CGG + chr1.2:24765399-24765418 MS.gene99234:CDS 30.0%
ATACTTTCCTGATACGTATT+GGG - chr1.2:24763442-24763461 None:intergenic 30.0%
ATCATATATCTAGGCTTCAT+AGG + chr1.2:24763980-24763999 MS.gene99234:intron 30.0%
ATTGAATTGTAACTATGTGC+AGG + chr1.2:24764796-24764815 MS.gene99234:intron 30.0%
CAACAATCCAATACTTAATG+GGG + chr1.2:24764642-24764661 MS.gene99234:intron 30.0%
CACTGTGAATAAAAGAGATT+GGG - chr1.2:24762470-24762489 None:intergenic 30.0%
CATTATACCCTTAGATGAAA+TGG + chr1.2:24765048-24765067 MS.gene99234:CDS 30.0%
CCAACAATCCAATACTTAAT+GGG + chr1.2:24764641-24764660 MS.gene99234:intron 30.0%
CCTAGATATATGATACGATA+AGG - chr1.2:24763974-24763993 None:intergenic 30.0%
CCTTATCGTATCATATATCT+AGG + chr1.2:24763971-24763990 MS.gene99234:intron 30.0%
GAAACTTGCATGTTGTTAAA+GGG + chr1.2:24764716-24764735 MS.gene99234:intron 30.0%
GATACGATAAGGATATGATA+TGG - chr1.2:24763963-24763982 None:intergenic 30.0%
TCACTGTGAATAAAAGAGAT+TGG - chr1.2:24762471-24762490 None:intergenic 30.0%
TGGTCACCACAAAAAAAAAT+CGG - chr1.2:24762981-24763000 None:intergenic 30.0%
TTAATCACATCTGAATAACC+AGG - chr1.2:24762859-24762878 None:intergenic 30.0%
TTGGATCATCTTTAGTAGAT+TGG - chr1.2:24765711-24765730 None:intergenic 30.0%
TTTCAAAAATGCTAAAGGAG+AGG + chr1.2:24765597-24765616 MS.gene99234:CDS 30.0%
! CATTTCATCTAAGGGTATAA+TGG - chr1.2:24765050-24765069 None:intergenic 30.0%
! CGATACTTTTCAAATACATC+TGG + chr1.2:24763495-24763514 MS.gene99234:intron 30.0%
! CTTTTAACCCCATTAAGTAT+TGG - chr1.2:24764652-24764671 None:intergenic 30.0%
! TCTCCTTTAGCATTTTTGAA+AGG - chr1.2:24765598-24765617 None:intergenic 30.0%
! TTGATGGTCTTACAAATTCA+GGG - chr1.2:24762504-24762523 None:intergenic 30.0%
! TTTGATGGTCTTACAAATTC+AGG - chr1.2:24762505-24762524 None:intergenic 30.0%
!! GCTATTGTTTTATTGTGTGA+AGG + chr1.2:24763741-24763760 MS.gene99234:intron 30.0%
!! GTGTTTTATTATCCATTTGC+AGG + chr1.2:24762679-24762698 MS.gene99234:CDS 30.0%
!!! ATAGCACCGATTTTTTTTTG+TGG + chr1.2:24762972-24762991 MS.gene99234:intron 30.0%
!!! TAGAGATGTTGATTTTGGTT+GGG + chr1.2:24765501-24765520 MS.gene99234:CDS 30.0%
!!! TTAGAGATGTTGATTTTGGT+TGG + chr1.2:24765500-24765519 MS.gene99234:CDS 30.0%
AAAAATGCTAAAGGAGAGGA+AGG + chr1.2:24765601-24765620 MS.gene99234:CDS 35.0%
AAAAGAAACTCGTCCAAACA+AGG - chr1.2:24762829-24762848 None:intergenic 35.0%
AACGAAGCAATGTTATAGCA+TGG - chr1.2:24764068-24764087 None:intergenic 35.0%
AATCGCATTACAACCAGATA+CGG + chr1.2:24765192-24765211 MS.gene99234:CDS 35.0%
AGTGTCTTGGACACTAAATA+TGG + chr1.2:24764028-24764047 MS.gene99234:intron 35.0%
ATATAAAAGCTAGCAACACC+AGG - chr1.2:24765574-24765593 None:intergenic 35.0%
ATCCATTTGCAGGTAGATTA+AGG + chr1.2:24762689-24762708 MS.gene99234:CDS 35.0%
CAGATATAGATGACCAAGAA+GGG + chr1.2:24762554-24762573 MS.gene99234:CDS 35.0%
GATACTTTCCTGATACGTAT+TGG - chr1.2:24763443-24763462 None:intergenic 35.0%
GCGGATTTAATGGTTATCAA+GGG + chr1.2:24765418-24765437 MS.gene99234:CDS 35.0%
GGAAACTTGCATGTTGTTAA+AGG + chr1.2:24764715-24764734 MS.gene99234:intron 35.0%
GTATTCAGTTGCGGATTTAA+TGG + chr1.2:24765408-24765427 MS.gene99234:CDS 35.0%
TATACCTGGATAAGAAGGAT+TGG - chr1.2:24762886-24762905 None:intergenic 35.0%
TCAGATATAGATGACCAAGA+AGG + chr1.2:24762553-24762572 MS.gene99234:CDS 35.0%
TCCATTTGCAGGTAGATTAA+GGG + chr1.2:24762690-24762709 MS.gene99234:CDS 35.0%
TGCAGTGATGATTTCGAATT+TGG - chr1.2:24765152-24765171 None:intergenic 35.0%
TGCGGATTTAATGGTTATCA+AGG + chr1.2:24765417-24765436 MS.gene99234:CDS 35.0%
TTAAAAAAGGTTCAGTCCAG+TGG + chr1.2:24763176-24763195 MS.gene99234:intron 35.0%
TTTCTATGTCTGTTTGGTCT+AGG + chr1.2:24763790-24763809 MS.gene99234:intron 35.0%
TTTGAAAACGTAACCCTTCT+TGG - chr1.2:24762570-24762589 None:intergenic 35.0%
! ATAATGGTTCCTTTTGTGTC+AGG - chr1.2:24765034-24765053 None:intergenic 35.0%
! CAAATACATCTGGGATACAA+GGG + chr1.2:24763505-24763524 MS.gene99234:intron 35.0%
! CGAATTTGGATTGTTGTTGT+AGG - chr1.2:24765138-24765157 None:intergenic 35.0%
! TCAAATACATCTGGGATACA+AGG + chr1.2:24763504-24763523 MS.gene99234:intron 35.0%
! TGATGGTCTTACAAATTCAG+GGG - chr1.2:24762503-24762522 None:intergenic 35.0%
!! CCCATTAAGTATTGGATTGT+TGG - chr1.2:24764644-24764663 None:intergenic 35.0%
!!! AGAGATGTTGATTTTGGTTG+GGG + chr1.2:24765502-24765521 MS.gene99234:CDS 35.0%
!!! GGACTTAGAGATGTTGATTT+TGG + chr1.2:24765496-24765515 MS.gene99234:CDS 35.0%
!!! TTTTTTGTGGTGACCAGAAT+TGG + chr1.2:24762985-24763004 MS.gene99234:intron 35.0%
AAATCATCACTGCATGCCTT+TGG + chr1.2:24765158-24765177 MS.gene99234:CDS 40.0%
AACTCAAGTGCATACCCTAA+TGG - chr1.2:24765349-24765368 None:intergenic 40.0%
AACTCGTCCAAACAAGGAAA+AGG - chr1.2:24762823-24762842 None:intergenic 40.0%
AAGACAAAACATCGGAGAAG+TGG - chr1.2:24764613-24764632 None:intergenic 40.0%
ACACAGAGACACAAAACAGA+CGG - chr1.2:24763706-24763725 None:intergenic 40.0%
AGAACGAAGTGCAGATAACT+CGG - chr1.2:24765101-24765120 None:intergenic 40.0%
ATAGAGAGCTTCTAAACGCA+AGG + chr1.2:24764963-24764982 MS.gene99234:CDS 40.0%
ATCTAGGCTTCATCAGAGAA+TGG - chr1.2:24763357-24763376 None:intergenic 40.0%
ATTCTCACCACGGTAAAATG+TGG - chr1.2:24765448-24765467 None:intergenic 40.0%
CACTAAGTACATTCTCACCA+CGG - chr1.2:24765458-24765477 None:intergenic 40.0%
CTGGTTGTAATGCGATTGTA+CGG - chr1.2:24765189-24765208 None:intergenic 40.0%
GAATGAACCTTCAATCTCAC+CGG + chr1.2:24764934-24764953 MS.gene99234:CDS 40.0%
GATACTTCCCAATACGTATC+AGG + chr1.2:24763432-24763451 MS.gene99234:intron 40.0%
GATGTTGAGTCTAGTGAAAG+AGG - chr1.2:24764108-24764127 None:intergenic 40.0%
TAAACTCTAACCTCATCCAC+TGG - chr1.2:24763195-24763214 None:intergenic 40.0%
TAGAGAGCTTCTAAACGCAA+GGG + chr1.2:24764964-24764983 MS.gene99234:CDS 40.0%
TCCCTTAATCTACCTGCAAA+TGG - chr1.2:24762694-24762713 None:intergenic 40.0%
TTGGACCGAAGAAAAAAGAG+CGG - chr1.2:24765078-24765097 None:intergenic 40.0%
TTTGCAGGTAGATTAAGGGA+AGG + chr1.2:24762694-24762713 MS.gene99234:CDS 40.0%
! ATAGTAACCTTTTGGCAACG+GGG - chr1.2:24765269-24765288 None:intergenic 40.0%
! CATAGTAACCTTTTGGCAAC+GGG - chr1.2:24765270-24765289 None:intergenic 40.0%
! CCATAGTAACCTTTTGGCAA+CGG - chr1.2:24765271-24765290 None:intergenic 40.0%
! GAGTTTCTTTTCGATGTTCC+TGG + chr1.2:24762838-24762857 MS.gene99234:CDS 40.0%
! GCGTTTCCATAGTAACCTTT+TGG - chr1.2:24765277-24765296 None:intergenic 40.0%
! GTATCGGTATAGGATACACA+GGG - chr1.2:24763406-24763425 None:intergenic 40.0%
! TATCGAGCATGTATCGGTAT+AGG - chr1.2:24763416-24763435 None:intergenic 40.0%
! TCAACCTCCTTTTCCTTGTT+TGG + chr1.2:24762813-24762832 MS.gene99234:CDS 40.0%
! TGTATCGGTATAGGATACAC+AGG - chr1.2:24763407-24763426 None:intergenic 40.0%
!! AAACTTATGGTGGATTGCAC+TGG + chr1.2:24762727-24762746 MS.gene99234:CDS 40.0%
!!! GAGATGTTGATTTTGGTTGG+GGG + chr1.2:24765503-24765522 MS.gene99234:CDS 40.0%
!!! TCATGAGGTGTTGGTTTTGA+TGG - chr1.2:24762520-24762539 None:intergenic 40.0%
AAAGTTCGGGGTTCCAATTC+TGG - chr1.2:24763001-24763020 None:intergenic 45.0%
AACCTTCAATCTCACCGGTA+TGG + chr1.2:24764939-24764958 MS.gene99234:CDS 45.0%
AGAAGCTCTCTATGCCATAC+CGG - chr1.2:24764956-24764975 None:intergenic 45.0%
AGGTCCTGCTAGGAAACTTA+TGG + chr1.2:24762714-24762733 MS.gene99234:CDS 45.0%
ATTACAACCAGATACGGACG+AGG + chr1.2:24765198-24765217 MS.gene99234:CDS 45.0%
CACTCCAATCCTTCTTATCC+AGG + chr1.2:24762879-24762898 MS.gene99234:CDS 45.0%
CCGTTGCCAAAAGGTTACTA+TGG + chr1.2:24765268-24765287 MS.gene99234:CDS 45.0%
CCTGCAGTTGTAATTGCAAC+AGG - chr1.2:24765310-24765329 None:intergenic 45.0%
CCTGTTGCAATTACAACTGC+AGG + chr1.2:24765307-24765326 MS.gene99234:CDS 45.0%
CCTTGGGTGAAATTTCTTGC+GGG + chr1.2:24764909-24764928 MS.gene99234:CDS 45.0%
CGATGAGTGCATGTTACTCT+TGG - chr1.2:24764995-24765014 None:intergenic 45.0%
CGGTGGACCATTTCATCTAA+GGG - chr1.2:24765058-24765077 None:intergenic 45.0%
CTCTTTAACGAACCTCTCCA+TGG - chr1.2:24765659-24765678 None:intergenic 45.0%
GAGCAAGTTCCTGACACAAA+AGG + chr1.2:24765022-24765041 MS.gene99234:CDS 45.0%
GCCTTGGGTGAAATTTCTTG+CGG + chr1.2:24764908-24764927 MS.gene99234:CDS 45.0%
GGGTCTTTCCCTTCCATATT+TGG - chr1.2:24762622-24762641 None:intergenic 45.0%
GTCCAATTCATGAGTGCCTT+GGG + chr1.2:24764893-24764912 MS.gene99234:CDS 45.0%
GTGTCTGATGTTACACATGC+TGG + chr1.2:24765475-24765494 MS.gene99234:CDS 45.0%
TCCACCATAAGTTTCCTAGC+AGG - chr1.2:24762721-24762740 None:intergenic 45.0%
TCCTGCTAGGAAACTTATGG+TGG + chr1.2:24762717-24762736 MS.gene99234:CDS 45.0%
TCGTCCAAACAAGGAAAAGG+AGG - chr1.2:24762820-24762839 None:intergenic 45.0%
TGAGTGCATGTTACTCTTGG+TGG - chr1.2:24764992-24765011 None:intergenic 45.0%
TGCTAAAGGAGAGGAAGGTT+TGG + chr1.2:24765606-24765625 MS.gene99234:CDS 45.0%
TTGTTTGCCAACTCAAGCCA+TGG + chr1.2:24765639-24765658 MS.gene99234:CDS 45.0%
! GATTGTTGTTGTAGGTGACG+TGG - chr1.2:24765130-24765149 None:intergenic 45.0%
! TAGTAACCTTTTGGCAACGG+GGG - chr1.2:24765268-24765287 None:intergenic 45.0%
! TCTGGGATACAAGGGATATG+CGG + chr1.2:24763513-24763532 MS.gene99234:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG - chr1.2:24763042-24763061 None:intergenic 45.0%
!! GCTTGAGTTGGCAAACAAAG+TGG - chr1.2:24765637-24765656 None:intergenic 45.0%
!! GGGAAGTATCGAGCATGTAT+CGG - chr1.2:24763422-24763441 None:intergenic 45.0%
!!! GTCCACCGCTCTTTTTTCTT+CGG + chr1.2:24765070-24765089 MS.gene99234:CDS 45.0%
!! TTTGATAAATTAATTAAAAT+TGG - chr1.2:24764411-24764430 None:intergenic 5.0%
!!! AACTATTTTATTTTATTTTT+TGG - chr1.2:24763292-24763311 None:intergenic 5.0%
AACCTCTCCATGGCTTGAGT+TGG - chr1.2:24765649-24765668 None:intergenic 50.0%
AAGGTTCAGTCCAGTGGATG+AGG + chr1.2:24763182-24763201 MS.gene99234:intron 50.0%
ATCAGGTAGCCCGCACATAT+CGG - chr1.2:24763546-24763565 None:intergenic 50.0%
CAGAGACACAAAACAGACGG+CGG - chr1.2:24763703-24763722 None:intergenic 50.0%
CAGGTAACACGTCTCAAGTG+TGG + chr1.2:24764815-24764834 MS.gene99234:intron 50.0%
CATACGATGAGCGATGCGAT+GGG + chr1.2:24764866-24764885 MS.gene99234:CDS 50.0%
CCCGCAAGAAATTTCACCCA+AGG - chr1.2:24764912-24764931 None:intergenic 50.0%
CGAAGTGCAGATAACTCGGT+TGG - chr1.2:24765097-24765116 None:intergenic 50.0%
CGGTGGAAAACCGATATGTG+CGG + chr1.2:24763533-24763552 MS.gene99234:intron 50.0%
GACCGAAGAAAAAAGAGCGG+TGG - chr1.2:24765075-24765094 None:intergenic 50.0%
GATTAAGGGAAGGTCCTGCT+AGG + chr1.2:24762704-24762723 MS.gene99234:CDS 50.0%
GCGGTGGACCATTTCATCTA+AGG - chr1.2:24765059-24765078 None:intergenic 50.0%
GGTCCAATTCATGAGTGCCT+TGG + chr1.2:24764892-24764911 MS.gene99234:CDS 50.0%
GGTGGAAAACCGATATGTGC+GGG + chr1.2:24763534-24763553 MS.gene99234:intron 50.0%
GGTTGGGGGAAAGCTGTATA+TGG + chr1.2:24765517-24765536 MS.gene99234:CDS 50.0%
GTAACACGTCTCAAGTGTGG+TGG + chr1.2:24764818-24764837 MS.gene99234:CDS 50.0%
GTATATGGAGGACCAGCTAC+AGG + chr1.2:24765532-24765551 MS.gene99234:CDS 50.0%
TCTCGTGAGCTTAGCTCAGT+TGG - chr1.2:24763047-24763066 None:intergenic 50.0%
TGCCATACCGGTGAGATTGA+AGG - chr1.2:24764944-24764963 None:intergenic 50.0%
TGGGGGAAAGCTGTATATGG+AGG + chr1.2:24765520-24765539 MS.gene99234:CDS 50.0%
TTCAATCCCCCGTTGCCAAA+AGG + chr1.2:24765259-24765278 MS.gene99234:CDS 50.0%
! CACCCAAGGCACTCATGAAT+TGG - chr1.2:24764898-24764917 None:intergenic 50.0%
!! ATGGTGGATTGCACTGGAGA+AGG + chr1.2:24762733-24762752 MS.gene99234:CDS 50.0%
!! TGGCATCGATACGCAGATAC+AGG - chr1.2:24763943-24763962 None:intergenic 50.0%
ACACCAACTCCTCCTGTAGC+TGG - chr1.2:24765547-24765566 None:intergenic 55.0%
CCATACGATGAGCGATGCGA+TGG + chr1.2:24764865-24764884 MS.gene99234:CDS 55.0%
CCATCGCATCGCTCATCGTA+TGG - chr1.2:24764868-24764887 None:intergenic 55.0%
CGAACTTCCTCGTCCGTATC+TGG - chr1.2:24765208-24765227 None:intergenic 55.0%
CGATTGTACGGCAACGCCAA+AGG - chr1.2:24765177-24765196 None:intergenic 55.0%
GGGATACAAGGGATATGCGG+TGG + chr1.2:24763516-24763535 MS.gene99234:intron 55.0%
TATGGAGGACCAGCTACAGG+AGG + chr1.2:24765535-24765554 MS.gene99234:CDS 55.0%
TGCCAACTCAAGCCATGGAG+AGG + chr1.2:24765644-24765663 MS.gene99234:CDS 55.0%
! GATGAGCGATGCGATGGGTT+TGG + chr1.2:24764871-24764890 MS.gene99234:CDS 55.0%
! GGGTCGACCACATTTTACCG+TGG + chr1.2:24765438-24765457 MS.gene99234:CDS 55.0%
!! GGAGGAGTTGGTGTTACACC+TGG + chr1.2:24765553-24765572 MS.gene99234:CDS 55.0%
GGACCAGCTACAGGAGGAGT+TGG + chr1.2:24765541-24765560 MS.gene99234:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 24762463 24765759 24762463 ID=MS.gene99234
chr1.2 mRNA 24762463 24765759 24762463 ID=MS.gene99234.t1;Parent=MS.gene99234
chr1.2 exon 24762463 24762900 24762463 ID=MS.gene99234.t1.exon1;Parent=MS.gene99234.t1
chr1.2 CDS 24762463 24762900 24762463 ID=cds.MS.gene99234.t1;Parent=MS.gene99234.t1
chr1.2 exon 24764818 24765759 24764818 ID=MS.gene99234.t1.exon2;Parent=MS.gene99234.t1
chr1.2 CDS 24764818 24765759 24764818 ID=cds.MS.gene99234.t1;Parent=MS.gene99234.t1
Gene Sequence

>MS.gene99234

ATGGCCCAATCTCTTTTATTCACAGTGAAGAGAAGTGCCCCTGAATTTGTAAGACCATCAAAACCAACACCTCATGAAATAAAATTACTCTCAGATATAGATGACCAAGAAGGGTTACGTTTTCAAATTCCAGTGATACAATTTTATAAATATGATCCAAATATGGAAGGGAAAGACCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGATTAAGGGAAGGTCCTGCTAGGAAACTTATGGTGGATTGCACTGGAGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATGTTAGTTTGAAAGAATTTGGTGACGCTCTTCAACCTCCTTTTCCTTGTTTGGACGAGTTTCTTTTCGATGTTCCTGGTTATTCAGATGTGATTAACACTCCAATCCTTCTTATCCAGGTATATTATTGATATTTTCATGATGTATCAAAGGTCTCTTAATTTCTCAAAAGAAATAGAGAAATTTATTTATAGCACCGATTTTTTTTTGTGGTGACCAGAATTGGAACCCCGAACTTTACATATATTATATATTGTCCATACCAACTGAGCTAAGCTCACGAGATCATAACACAAATCATTTGAAATTACAGTTTCATTTACTTAATCCCTTTCCAATAAAATATAAGCAAACTTAATTAGTTACACACTTATTAATATAACTAGTTATTTAATTAAAAAAGGTTCAGTCCAGTGGATGAGGTTAGAGTTTAGATTTATATATTGTACCGAGTTAATGTTTGAATATCGAATAGAATTGCTATAAATGGATAGCATTTAAAGGAAGCCAAAAAATAAAATAAAATAGTTATTCAAATTTATTACTGGATTCTTATATTTGATATATTCGTTCCATTCTCTGATGAAGCCTAGATACTTCAAAATGATTGACGTATCATATCCCTGTGTATCCTATACCGATACATGCTCGATACTTCCCAATACGTATCAGGAAAGTATCCGAATATTAATTTTTTTTTAAAAACAATTATCCGATACTTTTCAAATACATCTGGGATACAAGGGATATGCGGTGGAAAACCGATATGTGCGGGCTACCTGATGTTTCTATTATATTTGGTACATATGTAATTGACTAAGTAATGATGCTTTTTATTTATGATATATCATATATGTAATTAACTAATTGTCGCTTTCTTTATTATCAATATTATTTACATTTATATTTCTATTGTTGCCGCCGTCTGTTTTGTGTCTCTGTGTTTGTTTGAAAACAATGCTATTGTTTTATTGTGTGAAGGGAATTTCATTGTTGTGCCTTATTGTTTTTCTATGTCTGTTTGGTCTAGGTTTTGCGCCATTTTTTTTGCTTTTGAGTTTTAGTCCATATTTTGTATTTTGAGTTTTAATATGTAGTTTAAATTTAATTTTTTATTGAAGTATCTCAGCCGTATCGTATCTTAAATTTTAAAATGTTCCTGTATCTGCGTATCGATGCCATATCATATCCTTATCGTATCATATATCTAGGCTTCATAGGTTCCATTTGAAGTAATTATATTCAAAGTGTCTTGGACACTAAATATGGATATATCATTCGATCCATGCTATAACATTGCTTCGTTATGCTGAAAGAGTTTAACCTCTTTCACTAGACTCAACATCTATACTTACATTGCACTTTCATTTTATTTAACTATATATATATAGAAACAAGTGGACATTTAATTTAACAAAATTGAATTTTAAATTAAGGACATCTTTAATATACTATCATTGAATAGATGTATTTCTCAAAAAAAAAAAATATCATTGAATATTGAATAGATGCATTTGTCAACAAAAAAAAATCATTGAATAGATGTAGAAAAGTCGAAATTTGTTTATAACCTAATTAATTAACTCAAATCTTGTATTCACAATCACAAGTGTTTAATTATGGGGTCCAATTTTAATTAATTTATCAAAATAAAATTATGATTAGGAAAATACTAAATGGTACGACAATGTTTTCGCTTTAATTGCACGACACTACTAACTTGCTTCTTTTTTAATTTAAATATAACTTAAAATAATGCATTCAATTCCTGATTTATTCTTTTACAAGAAATCACAAGTTATAAATTACTAGTACATCATATTTCTTGCCACTTCTCCGATGTTTTGTCTTGTTTGATACCAACAATCCAATACTTAATGGGGTTAAAAGAGTTTTGAGTTTTGATCTTAAAGTCTTTGATTTTCTTTTTATGGGAAACTTGCATGTTGTTAAAGGGTTCGTGCGTATTCGTGTGTCAAGTATGTCGCACCATATATATCACATCTCTTACATAATTGAATTGTAACTATGTGCAGGTAACACGTCTCAAGTGTGGTGGTTTCATATTTGCTCTTCGTCTAAACCATACGATGAGCGATGCGATGGGTTTGGTCCAATTCATGAGTGCCTTGGGTGAAATTTCTTGCGGGATGAATGAACCTTCAATCTCACCGGTATGGCATAGAGAGCTTCTAAACGCAAGGGATCCACCAAGAGTAACATGCACTCATCGTGAATACGAGCAAGTTCCTGACACAAAAGGAACCATTATACCCTTAGATGAAATGGTCCACCGCTCTTTTTTCTTCGGTCCAACCGAGTTATCTGCACTTCGTTCTCTATTTCCACGTCACCTACAACAACAATCCAAATTCGAAATCATCACTGCATGCCTTTGGCGTTGCCGTACAATCGCATTACAACCAGATACGGACGAGGAAGTTCGCATAATTTGCTTATACAATGCACGTCGCAAGTTCAATCCCCCGTTGCCAAAAGGTTACTATGGAAACGCTTTTGCGTTTCCTGTTGCAATTACAACTGCAGGAAAACTAATTGAAAATCCATTAGGGTATGCACTTGAGTTAGTTAAAAAAGCTAAATCAGATGTCGCTCAAGAATACATGTATTCAGTTGCGGATTTAATGGTTATCAAGGGTCGACCACATTTTACCGTGGTGAGAATGTACTTAGTGTCTGATGTTACACATGCTGGACTTAGAGATGTTGATTTTGGTTGGGGGAAAGCTGTATATGGAGGACCAGCTACAGGAGGAGTTGGTGTTACACCTGGTGTTGCTAGCTTTTATATACCTTTCAAAAATGCTAAAGGAGAGGAAGGTTTGGTTATACCACTTTGTTTGCCAACTCAAGCCATGGAGAGGTTCGTTAAAGAGTTAGATAGTTTGCTTAAGAGAAACATTAGCCAATCTACTAAAGATGATCCAAAATATGCTATCATTAATTCTTCGTTGTAG
Protein sequence

>MS.gene99234.t1

MAQSLLFTVKRSAPEFVRPSKPTPHEIKLLSDIDDQEGLRFQIPVIQFYKYDPNMEGKDPVDIIRKALAKTLVFYYPFAGRLREGPARKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDEFLFDVPGYSDVINTPILLIQVTRLKCGGFIFALRLNHTMSDAMGLVQFMSALGEISCGMNEPSISPVWHRELLNARDPPRVTCTHREYEQVPDTKGTIIPLDEMVHRSFFFGPTELSALRSLFPRHLQQQSKFEIITACLWRCRTIALQPDTDEEVRIICLYNARRKFNPPLPKGYYGNAFAFPVAITTAGKLIENPLGYALELVKKAKSDVAQEYMYSVADLMVIKGRPHFTVVRMYLVSDVTHAGLRDVDFGWGKAVYGGPATGGVGVTPGVASFYIPFKNAKGEEGLVIPLCLPTQAMERFVKELDSLLKRNISQSTKDDPKYAIINSSL