AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99236

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99236.t1 MTR_1g040175 85.249 461 65 1 1 461 1 458 0.0 809
MS.gene99236.t1 MTR_1g040320 87.419 461 22 1 1 461 1 425 0.0 805
MS.gene99236.t1 MTR_1g040215 76.356 461 109 0 1 461 1 461 0.0 738
MS.gene99236.t1 MTR_1g040195 71.800 461 105 3 1 461 1 436 0.0 667
MS.gene99236.t1 MTR_1g040315 65.076 461 117 4 1 461 1 417 0.0 585
MS.gene99236.t1 MTR_7g114560 55.507 454 195 6 5 455 6 455 0.0 521
MS.gene99236.t1 MTR_7g114550 54.367 458 202 6 2 456 3 456 0.0 521
MS.gene99236.t1 MTR_7g114520 55.778 450 188 7 5 448 7 451 1.91e-180 513
MS.gene99236.t1 MTR_5g076700 55.066 454 196 6 5 454 6 455 1.28e-179 510
MS.gene99236.t1 MTR_7g114470 53.846 455 201 6 5 454 7 457 7.70e-175 498
MS.gene99236.t1 MTR_7g114490 54.398 432 189 5 5 432 6 433 8.02e-170 487
MS.gene99236.t1 MTR_1g081660 56.720 439 185 5 4 440 13 448 1.34e-169 484
MS.gene99236.t1 MTR_0042s0190 73.401 297 79 0 165 461 1 297 7.46e-162 459
MS.gene99236.t1 MTR_0042s0190 74.569 232 59 0 230 461 1 232 1.21e-123 359
MS.gene99236.t1 MTR_7g114510 51.466 307 139 6 147 448 8 309 1.07e-101 306
MS.gene99236.t1 MTR_0502s0010 57.438 242 95 4 100 337 1 238 1.19e-92 280
MS.gene99236.t1 MTR_3g035000 62.162 222 57 3 199 419 43 238 3.06e-85 264
MS.gene99236.t1 MTR_0042s0230 82.993 147 25 0 1 147 1 147 2.26e-82 251
MS.gene99236.t1 MTR_3g098980 32.867 429 269 10 9 432 14 428 3.34e-78 250
MS.gene99236.t1 MTR_7g114420 44.558 294 128 6 165 454 1 263 2.12e-77 243
MS.gene99236.t1 MTR_7g027175 33.097 423 265 10 13 430 20 429 2.99e-75 243
MS.gene99236.t1 MTR_8g096530 32.798 436 271 12 1 430 1 420 7.66e-75 242
MS.gene99236.t1 MTR_8g039950 79.688 128 26 0 1 128 1 128 2.16e-68 214
MS.gene99236.t1 MTR_7g114500 60.000 150 60 0 2 151 3 152 1.73e-62 200
MS.gene99236.t1 MTR_3g051790 61.074 149 58 0 2 150 6 154 1.37e-58 191
MS.gene99236.t1 MTR_3g112180 33.162 389 232 12 17 391 20 394 1.08e-54 189
MS.gene99236.t1 MTR_1g053315 30.023 443 286 12 17 440 26 463 1.65e-54 189
MS.gene99236.t1 MTR_4g055170 29.204 452 299 10 3 437 6 453 1.93e-54 189
MS.gene99236.t1 MTR_4g007540 30.516 426 270 12 9 420 3 416 1.18e-53 186
MS.gene99236.t1 MTR_8g075610 29.977 427 253 11 16 420 1 403 2.86e-52 182
MS.gene99236.t1 MTR_2g105330 28.864 440 287 12 9 436 3 428 9.89e-51 178
MS.gene99236.t1 MTR_2g064495 28.864 440 287 12 9 436 3 428 6.21e-50 176
MS.gene99236.t1 MTR_7g110610 30.529 416 220 13 5 411 1 356 2.22e-46 165
MS.gene99236.t1 MTR_3g071320 30.051 396 259 10 9 391 5 395 2.94e-46 166
MS.gene99236.t1 MTR_3g465990 29.362 470 283 17 3 439 4 457 3.82e-44 161
MS.gene99236.t1 MTR_7g084940 28.426 394 267 9 5 391 12 397 2.15e-43 159
MS.gene99236.t1 MTR_3g465930 28.017 464 293 14 7 439 8 461 2.94e-41 153
MS.gene99236.t1 MTR_3g465910 28.993 407 261 12 7 392 8 407 2.09e-40 152
MS.gene99236.t1 MTR_3g465980 29.130 460 285 16 9 439 10 457 2.39e-40 150
MS.gene99236.t1 MTR_7g085990 28.112 466 279 18 9 434 2 451 3.31e-40 150
MS.gene99236.t1 MTR_7g085930 28.066 424 257 15 9 392 6 421 3.33e-40 150
MS.gene99236.t1 MTR_7g086000 29.803 406 238 16 17 385 12 407 1.21e-39 149
MS.gene99236.t1 MTR_7g084910 27.708 397 246 11 8 391 16 384 2.01e-39 147
MS.gene99236.t1 MTR_6g053810 25.957 470 303 14 3 439 5 462 2.06e-39 148
MS.gene99236.t1 MTR_7g056087 28.315 445 271 16 16 434 11 433 2.80e-39 147
MS.gene99236.t1 MTR_2g015430 30.155 388 257 10 9 387 2 384 1.80e-38 145
MS.gene99236.t1 MTR_3g026950 26.961 408 258 10 17 392 10 409 4.07e-38 144
MS.gene99236.t1 MTR_4g127650 27.295 414 264 12 8 392 9 414 2.62e-37 142
MS.gene99236.t1 MTR_3g465950 29.016 386 252 11 7 378 8 385 1.00e-35 138
MS.gene99236.t1 MTR_3g465880 27.660 470 290 16 7 439 8 464 1.79e-34 134
MS.gene99236.t1 MTR_3g049150 26.980 404 255 12 17 390 10 403 1.96e-33 131
MS.gene99236.t1 MTR_4g097090 26.959 434 275 14 17 420 17 438 3.66e-33 130
MS.gene99236.t1 MTR_4g088765 78.082 73 16 0 1 73 1 73 5.18e-32 118
MS.gene99236.t1 MTR_4g013050 25.629 437 254 16 17 430 12 400 8.01e-31 123
MS.gene99236.t1 MTR_5g090830 26.683 401 232 14 61 427 63 435 2.22e-29 120
MS.gene99236.t1 MTR_5g090810 26.434 401 233 14 61 427 63 435 1.10e-28 118
MS.gene99236.t1 MTR_3g465870 29.452 292 189 9 5 283 8 295 6.70e-27 112
MS.gene99236.t1 MTR_5g090880 26.119 402 234 15 61 427 64 437 6.07e-26 110
MS.gene99236.t1 MTR_1g094790 26.411 443 257 20 15 419 9 420 2.37e-25 108
MS.gene99236.t1 MTR_5g084870 24.250 400 246 15 15 388 9 377 8.39e-25 106
MS.gene99236.t1 MTR_3g450710 26.316 437 235 18 64 454 65 460 9.33e-25 107
MS.gene99236.t1 MTR_3g450690 25.814 430 243 17 64 454 72 464 1.11e-23 103
MS.gene99236.t1 MTR_3g070170 26.573 429 242 16 53 432 55 459 1.46e-23 103
MS.gene99236.t1 MTR_8g467590 24.757 412 273 15 15 405 9 404 7.09e-23 101
MS.gene99236.t1 MTR_3g086020 24.433 397 241 15 15 390 11 369 5.44e-22 98.2
MS.gene99236.t1 MTR_4g094842 26.098 387 231 16 48 399 46 412 9.09e-21 94.7
MS.gene99236.t1 MTR_8g041770 25.753 365 237 14 49 395 49 397 1.26e-20 94.4
MS.gene99236.t1 MTR_8g467580 24.634 410 276 15 15 405 9 404 2.74e-20 93.6
MS.gene99236.t1 MTR_4g094828 24.257 404 271 13 51 432 49 439 2.85e-20 93.2
MS.gene99236.t1 MTR_8g079970 23.020 404 252 11 15 388 9 383 8.16e-20 92.0
MS.gene99236.t1 MTR_5g084850 22.277 404 248 13 15 388 9 376 1.84e-19 90.9
MS.gene99236.t1 MTR_8g079950 33.333 180 102 6 15 187 9 177 4.85e-19 89.7
MS.gene99236.t1 MTR_8g079710 25.833 360 221 15 57 387 52 394 4.46e-18 86.7
MS.gene99236.t1 MTR_3g101930 26.446 363 223 15 64 397 70 417 5.33e-18 86.7
MS.gene99236.t1 MTR_8g079980 26.523 279 171 10 15 278 9 268 6.41e-18 86.3
MS.gene99236.t1 MTR_5g084900 22.156 334 215 11 61 388 6 300 8.63e-18 85.1
MS.gene99236.t1 MTR_7g026860 26.108 406 259 18 48 432 42 427 2.69e-17 84.3
MS.gene99236.t1 MTR_4g094838 22.162 370 239 12 53 399 53 396 1.24e-16 82.0
MS.gene99236.t1 MTR_3g101900 24.578 415 254 18 61 445 63 448 1.84e-16 81.6
MS.gene99236.t1 MTR_7g114417 65.385 52 17 1 89 139 29 80 1.44e-15 72.0
MS.gene99236.t1 MTR_3g102010 25.326 383 235 17 54 402 55 420 1.67e-15 79.0
MS.gene99236.t1 MTR_4g022800 25.397 315 206 10 23 323 25 324 1.77e-15 78.6
MS.gene99236.t1 MTR_3g102020 24.533 375 230 15 61 402 66 420 1.06e-14 76.3
MS.gene99236.t1 MTR_4g022825 25.062 403 250 17 59 432 50 429 1.18e-14 76.3
MS.gene99236.t1 MTR_2g036780 23.785 391 238 11 72 437 74 429 7.51e-14 73.6
MS.gene99236.t1 MTR_3g051820 48.276 87 41 3 367 452 1 84 8.04e-14 67.4
MS.gene99236.t1 MTR_3g049140 32.000 150 83 5 257 390 54 200 2.41e-13 70.1
MS.gene99236.t1 MTR_4g022760 27.372 274 181 9 64 323 55 324 6.07e-13 70.5
MS.gene99236.t1 MTR_1g035890 24.268 239 150 6 79 303 82 303 3.88e-12 68.6
MS.gene99236.t1 MTR_4g007460 31.206 141 87 4 78 218 1 131 1.96e-11 62.4
MS.gene99236.t1 MTR_7g114960 22.713 317 223 7 45 351 44 348 4.04e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99236.t1 AT5G17540 54.390 467 201 8 1 461 1 461 3.47e-173 494
MS.gene99236.t1 AT3G03480 52.489 442 199 8 5 441 15 450 1.60e-153 444
MS.gene99236.t1 AT5G41040 34.644 407 243 11 7 404 31 423 2.37e-80 256
MS.gene99236.t1 AT5G41040 34.644 407 243 11 7 404 15 407 3.12e-80 256
MS.gene99236.t1 AT5G63560 32.952 437 267 12 1 430 1 418 2.90e-78 250
MS.gene99236.t1 AT3G48720 30.805 435 283 10 1 430 1 422 1.20e-76 246
MS.gene99236.t1 AT1G27620 29.933 451 287 12 10 450 10 441 2.21e-53 185
MS.gene99236.t1 AT5G48930 30.269 446 277 13 8 436 2 430 3.08e-53 185
MS.gene99236.t1 AT3G62160 33.500 400 236 12 5 394 1 380 8.24e-53 183
MS.gene99236.t1 AT1G03390 31.726 394 244 12 14 391 27 411 1.02e-48 173
MS.gene99236.t1 AT5G57840 28.571 413 260 11 8 397 2 402 4.98e-48 171
MS.gene99236.t1 AT1G28680 28.125 448 293 13 13 441 8 445 2.61e-46 167
MS.gene99236.t1 AT2G19070 29.756 410 252 13 12 392 7 409 1.10e-45 165
MS.gene99236.t1 AT2G40230 31.436 404 237 15 9 390 5 390 3.78e-44 160
MS.gene99236.t1 AT1G65450 29.011 455 281 16 7 440 16 449 2.42e-43 159
MS.gene99236.t1 AT1G28680 28.997 369 242 10 13 368 8 369 6.17e-42 153
MS.gene99236.t1 AT3G47170 27.215 474 296 14 3 440 6 466 3.18e-40 150
MS.gene99236.t1 AT5G07080 29.353 402 249 11 9 387 14 403 1.71e-39 148
MS.gene99236.t1 AT2G23510 28.480 467 285 16 1 439 5 450 1.37e-38 145
MS.gene99236.t1 AT2G25150 26.889 450 304 13 9 441 13 454 5.87e-38 144
MS.gene99236.t1 AT1G32910 28.148 405 250 13 62 441 63 451 2.82e-34 134
MS.gene99236.t1 AT1G78990 28.165 387 231 13 62 431 65 421 2.17e-33 131
MS.gene99236.t1 AT1G31490 28.418 373 224 13 38 389 36 386 4.11e-30 122
MS.gene99236.t1 AT4G31910 28.608 395 236 15 25 384 24 407 3.96e-26 110
MS.gene99236.t1 AT1G24420 27.000 400 236 15 15 388 11 380 3.37e-25 107
MS.gene99236.t1 AT1G24420 27.000 400 236 15 15 388 11 380 3.37e-25 107
MS.gene99236.t1 AT3G26040 24.874 398 254 14 15 390 9 383 3.70e-24 104
MS.gene99236.t1 AT5G16410 25.496 353 220 12 62 390 89 422 1.75e-22 100
MS.gene99236.t1 AT1G24430 25.979 281 174 8 43 315 41 295 3.89e-22 99.0
MS.gene99236.t1 AT5G23970 25.167 449 270 16 8 431 2 409 4.86e-22 98.6
MS.gene99236.t1 AT5G38130 26.119 402 248 15 23 399 48 425 8.10e-22 98.2
MS.gene99236.t1 AT5G07860 26.396 394 246 12 64 437 72 441 1.75e-21 97.1
MS.gene99236.t1 AT5G07850 27.123 365 228 15 64 402 70 422 9.46e-21 94.7
MS.gene99236.t1 AT3G50280 25.140 358 237 13 64 399 59 407 8.33e-20 92.0
MS.gene99236.t1 AT5G07870 26.870 361 240 10 64 402 72 430 1.00e-19 92.0
MS.gene99236.t1 AT3G50270 25.698 358 237 11 64 399 62 412 2.94e-19 90.5
MS.gene99236.t1 AT5G42830 25.822 426 247 14 21 402 18 418 6.89e-19 89.4
MS.gene99236.t1 AT5G67160 25.055 455 281 16 13 437 2 426 1.87e-18 87.8
MS.gene99236.t1 AT1G65450 25.497 302 188 12 158 440 2 285 4.88e-17 81.6
MS.gene99236.t1 AT3G30280 23.171 410 261 17 7 388 4 387 3.29e-16 80.9
MS.gene99236.t1 AT5G47950 24.920 313 194 12 7 309 4 285 6.53e-13 70.9
MS.gene99236.t1 AT3G23840 24.399 291 172 9 80 353 81 340 1.52e-12 69.7
MS.gene99236.t1 AT3G29670 23.919 347 231 15 64 387 66 402 5.07e-12 68.2
MS.gene99236.t1 AT1G65450 34.014 147 92 4 7 151 16 159 5.79e-12 64.3
MS.gene99236.t1 AT2G39980 28.082 146 103 2 53 197 51 195 3.10e-11 65.9

Find 104 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCCATTTCCTTTCT+TGG 0.075005 1.2:+24772151 MS.gene99236:CDS
TGCAGCAATGATTTCAAAAT+TGG 0.167630 1.2:-24774250 None:intergenic
GGGTCTTTCCTTGCCATATT+TGG 0.168682 1.2:-24771957 None:intergenic
TTTGATGGTGTTATCAATTC+AGG 0.170516 1.2:-24771840 None:intergenic
TCTCCTTTATCATTTGTAAA+AGG 0.217002 1.2:-24774699 None:intergenic
ACTAAGGGTGATCACAAATT+TGG 0.227915 1.2:+24774819 MS.gene99236:CDS
GTTCCTTTGTTGACGGGTTC+TGG 0.244415 1.2:-24774123 None:intergenic
GATGTTACTCTTAAAGAATT+TGG 0.276060 1.2:+24772119 MS.gene99236:CDS
TTATAACCATGATCCAAATA+TGG 0.294228 1.2:+24771944 MS.gene99236:CDS
CACTTTGAATACTAGAGATT+TGG 0.294557 1.2:-24771805 None:intergenic
GGATTTAGAGATGTTGATTT+TGG 0.298021 1.2:+24774600 MS.gene99236:CDS
TATAATCCCGCAGTAGTTAA+AGG 0.315636 1.2:-24774552 None:intergenic
GAGCTTCTTTATGATGTTCC+TGG 0.318598 1.2:+24772176 MS.gene99236:CDS
ATCCATTTGCAGGTAGGTTA+AGG 0.320236 1.2:+24772027 MS.gene99236:CDS
AAATCATTGCTGCATACTTT+TGG 0.324455 1.2:+24774259 MS.gene99236:CDS
CAACTTCCAAATGGTTACTA+TGG 0.337065 1.2:+24774372 MS.gene99236:CDS
GGAGGGGATGGTGCTCTTCC+TGG 0.355512 1.2:+24774657 MS.gene99236:CDS
GTTCACCGCACTTTCTTCAT+TGG 0.359527 1.2:+24774168 MS.gene99236:CDS
CCTGCAGTTGTAACCGCAAC+GGG 0.366367 1.2:-24774411 None:intergenic
CCCGTTGCGGTTACAACTGC+AGG 0.385362 1.2:+24774411 MS.gene99236:CDS
CACTGAATGCATATACTCTT+TGG 0.396096 1.2:-24774499 None:intergenic
ACAAATGATAAAGGAGAGAA+AGG 0.408216 1.2:+24774705 MS.gene99236:CDS
TCCTTGCCATATTTGGATCA+TGG 0.409662 1.2:-24771950 None:intergenic
AGCTCATCCAAGAAAGGAAA+TGG 0.411901 1.2:-24772158 None:intergenic
AATTTGTGATCACCCTTAGT+AGG 0.417980 1.2:-24774816 None:intergenic
GCGGTGAACCATGTCGTCTA+AGG 0.420378 1.2:-24774154 None:intergenic
TCCATTTGCAGGTAGGTTAA+GGG 0.425790 1.2:+24772028 MS.gene99236:CDS
AGGTCCTAGTAGGAAACTTA+TGG 0.427564 1.2:+24772052 MS.gene99236:CDS
TCCCTTAACCTACCTGCAAA+TGG 0.437418 1.2:-24772029 None:intergenic
AGTAACCATTTGGAAGTTGT+TGG 0.444242 1.2:-24774368 None:intergenic
CAACATTAACCAGCCTACTA+AGG 0.446515 1.2:+24774803 MS.gene99236:CDS
AATTGGAGTATTGTTGCAAT+TGG 0.448429 1.2:-24774233 None:intergenic
GCGTTACCATAGTAACCATT+TGG 0.448893 1.2:-24774378 None:intergenic
CGTCCTCCTTTAACTACTGC+GGG 0.454902 1.2:+24774546 MS.gene99236:CDS
CACTCCATTGCTACTTATCC+AGG 0.458160 1.2:+24772217 MS.gene99236:CDS
CTCCACACCGGTGAGATTGA+AGG 0.462106 1.2:-24774039 None:intergenic
AACATGGTTCCTTTGTTGAC+GGG 0.466891 1.2:-24774129 None:intergenic
GGAGAGGTTGGTTAAAGAGT+TGG 0.469038 1.2:+24774764 MS.gene99236:CDS
TGTGATCACCCTTAGTAGGC+TGG 0.470105 1.2:-24774812 None:intergenic
CTTGCCAACGTAATGAATCT+TGG 0.470742 1.2:-24771905 None:intergenic
CTGATGCATCACTCAATGTA+TGG 0.482975 1.2:-24773963 None:intergenic
AAACTTATGGTTGATTGTAA+TGG 0.490791 1.2:+24772065 MS.gene99236:CDS
GCACCATCCCCTCCTTTAGC+TGG 0.492161 1.2:-24774648 None:intergenic
TTGATGGTGTTATCAATTCA+GGG 0.493333 1.2:-24771839 None:intergenic
CGCTTTAGCATTTCCCGTTG+CGG 0.494082 1.2:+24774398 MS.gene99236:CDS
GTGTCTGATGTTACACGTGC+TGG 0.496612 1.2:+24774579 MS.gene99236:CDS
AAAACTTATTGAAAATCCAT+TGG 0.514939 1.2:+24774434 MS.gene99236:CDS
TGAGTACATGTCACTCTTGG+TGG 0.517340 1.2:-24774087 None:intergenic
TATTGTTGCAATTGGTGAGT+TGG 0.518445 1.2:-24774225 None:intergenic
TGGTTATAAAATTGTATCAA+AGG 0.519033 1.2:-24771930 None:intergenic
GGTTAAGGGAAGGTCCTAGT+AGG 0.521835 1.2:+24772042 MS.gene99236:CDS
GTTGACCAACAACTTCCAAA+TGG 0.522277 1.2:+24774363 MS.gene99236:CDS
TGATAAAGGAGAGAAAGGTT+TGG 0.523809 1.2:+24774710 MS.gene99236:CDS
AACTCAAGTCATGGAGAGGT+TGG 0.531928 1.2:+24774752 MS.gene99236:CDS
TAAAGAAGCTCATCCAAGAA+AGG 0.532338 1.2:-24772164 None:intergenic
AACAGATTTGCTACGTGCAT+CGG 0.534097 1.2:-24774343 None:intergenic
AACCTCTCCATGACTTGAGT+TGG 0.536373 1.2:-24774750 None:intergenic
TCAACCATAAGTTTCCTACT+AGG 0.537063 1.2:-24772056 None:intergenic
CATGTTTCCCTTAGACGACA+TGG 0.537703 1.2:+24774146 MS.gene99236:CDS
CCCGCGAGATATCTCTCCTA+AGG 0.540283 1.2:-24774007 None:intergenic
TCCTGCAGTTGTAACCGCAA+CGG 0.546523 1.2:-24774412 None:intergenic
CAATGAGTACATGTCACTCT+TGG 0.551648 1.2:-24774090 None:intergenic
AAACATGGTTCCTTTGTTGA+CGG 0.552807 1.2:-24774130 None:intergenic
GCAGAGAGCTTCTTAATGCA+AGG 0.554177 1.2:+24774061 MS.gene99236:CDS
CTCTAGTATTCAAAGTGAAG+AGG 0.559433 1.2:+24771811 MS.gene99236:CDS
TTTATTATCCATTTGCAGGT+AGG 0.559740 1.2:+24772021 MS.gene99236:CDS
GTTTATGGAGGACCAGCTAA+AGG 0.560640 1.2:+24774636 MS.gene99236:CDS
CGGTGAACCATGTCGTCTAA+GGG 0.561272 1.2:-24774153 None:intergenic
CATACATTGAGTGATGCATC+AGG 0.566369 1.2:+24773964 MS.gene99236:CDS
CGAATTGCAGCAACCTCGGT+GGG 0.573531 1.2:-24774192 None:intergenic
AAATTCAATGCATACGCCAA+TGG 0.574953 1.2:-24774450 None:intergenic
TGCCAACTCAAGTCATGGAG+AGG 0.581695 1.2:+24774748 MS.gene99236:CDS
ACAACTAGATTCTAACGAAG+AGG 0.582842 1.2:+24774302 MS.gene99236:CDS
TCGTCCTCCTTTAACTACTG+CGG 0.583673 1.2:+24774545 MS.gene99236:CDS
GAGTAAACCTTCAATCTCAC+CGG 0.590238 1.2:+24774032 MS.gene99236:CDS
AACATTAACCAGCCTACTAA+GGG 0.592477 1.2:+24774804 MS.gene99236:CDS
GGACCAGCTAAAGGAGGGGA+TGG 0.594000 1.2:+24774645 MS.gene99236:CDS
AGAGCGAATTGCAGCAACCT+CGG 0.595184 1.2:-24774196 None:intergenic
CATGTCGTCTAAGGGAAACA+TGG 0.598974 1.2:-24774145 None:intergenic
ATGGAGGACCAGCTAAAGGA+GGG 0.603039 1.2:+24774640 MS.gene99236:CDS
ACCATGATCCAAATATGGCA+AGG 0.603800 1.2:+24771949 MS.gene99236:CDS
ATATGAAAGACAGCTTGACC+AGG 0.606019 1.2:-24774675 None:intergenic
TATACCTGGATAAGTAGCAA+TGG 0.608440 1.2:-24772221 None:intergenic
GTTGCAATTGGTGAGTTGGA+AGG 0.610618 1.2:-24774221 None:intergenic
CAGAGAGCTTCTTAATGCAA+GGG 0.614681 1.2:+24774062 MS.gene99236:CDS
ATGGTTGATTGTAATGGTGA+AGG 0.618125 1.2:+24772071 MS.gene99236:CDS
GCCTTAGGAGAGATATCTCG+CGG 0.618905 1.2:+24774006 MS.gene99236:CDS
ATGACCAAGATTCATTACGT+TGG 0.619237 1.2:+24771901 MS.gene99236:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.622508 1.2:+24774639 MS.gene99236:CDS
GATCCAGAACCCGTCAACAA+AGG 0.628800 1.2:+24774120 MS.gene99236:CDS
CAGGTAACACGCTTCAAGTG+TGG 0.636908 1.2:+24773913 MS.gene99236:intron
CAAAGAGTATATGCATTCAG+TGG 0.642688 1.2:+24774500 MS.gene99236:CDS
GTAACACGCTTCAAGTGTGG+TGG 0.643774 1.2:+24773916 MS.gene99236:CDS
AACCTTCAATCTCACCGGTG+TGG 0.645966 1.2:+24774037 MS.gene99236:CDS
TCATCCAAGAAAGGAAATGG+AGG 0.650133 1.2:-24772155 None:intergenic
TTTGCAGGTAGGTTAAGGGA+AGG 0.651163 1.2:+24772032 MS.gene99236:CDS
AGAAGCTCTCTGCTCCACAC+CGG 0.659501 1.2:-24774051 None:intergenic
TTAATCACATCTGAAGAACC+AGG 0.665982 1.2:-24772194 None:intergenic
GCGAATTGCAGCAACCTCGG+TGG 0.672845 1.2:-24774193 None:intergenic
TGGGACCAATGAAGAAAGTG+CGG 0.689546 1.2:-24774173 None:intergenic
CCTTAGGAGAGATATCTCGC+GGG 0.690826 1.2:+24774007 MS.gene99236:CDS
AATCCCGCAGTAGTTAAAGG+AGG 0.692126 1.2:-24774549 None:intergenic
TTTCTTCATTGGTCCCACCG+AGG 0.698432 1.2:+24774179 MS.gene99236:CDS
TGGAGGACCAGCTAAAGGAG+GGG 0.700713 1.2:+24774641 MS.gene99236:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCCTAAAAAAAAAAAAAAA+AGG + chr1.2:24773158-24773177 MS.gene99236:intron 10.0%
!!! AACCTTTTTTTTTTTTTTTT+AGG - chr1.2:24773163-24773182 None:intergenic 10.0%
!!! TAAATTATTTTAATAAGTGA+AGG - chr1.2:24773661-24773680 None:intergenic 10.0%
!!! TATTTGAATCATTTTTTTTA+AGG - chr1.2:24773723-24773742 None:intergenic 10.0%
!! AAAAATGATTCAAATATATG+TGG + chr1.2:24773727-24773746 MS.gene99236:intron 15.0%
!! TATTAAAATCAATTGATATG+CGG - chr1.2:24773856-24773875 None:intergenic 15.0%
!!! AAAAAACCTTATTTTCTATA+TGG - chr1.2:24773140-24773159 None:intergenic 15.0%
!!! AATAATTTAAACTTTTTGTG+AGG + chr1.2:24773672-24773691 MS.gene99236:intron 15.0%
!!! CAATTTATTTTATCTTTTCA+AGG + chr1.2:24773397-24773416 MS.gene99236:intron 15.0%
!!! CCAACATAAAATATTTTATT+AGG + chr1.2:24773355-24773374 MS.gene99236:intron 15.0%
!!! CCTAATAAAATATTTTATGT+TGG - chr1.2:24773358-24773377 None:intergenic 15.0%
!! AAAACTTATTGAAAATCCAT+TGG + chr1.2:24774434-24774453 MS.gene99236:CDS 20.0%
!! AAGAAATAAATAACATTAGG+AGG - chr1.2:24772799-24772818 None:intergenic 20.0%
!! AGAAATAAATAACATTAGGA+GGG - chr1.2:24772798-24772817 None:intergenic 20.0%
!! ATACAAAATTATCCTCATTT+AGG - chr1.2:24772744-24772763 None:intergenic 20.0%
!! TACAAAATTATCCTCATTTA+GGG - chr1.2:24772743-24772762 None:intergenic 20.0%
!! TGGAAGAAATAAATAACATT+AGG - chr1.2:24772802-24772821 None:intergenic 20.0%
!! TGGTTATAAAATTGTATCAA+AGG - chr1.2:24771933-24771952 None:intergenic 20.0%
!! TGTTAAAAATGGTCATTAAT+TGG - chr1.2:24773755-24773774 None:intergenic 20.0%
!!! ATCAAATTTTGAGATCATTT+TGG - chr1.2:24773580-24773599 None:intergenic 20.0%
!!! ATCAAGAGTATTTAAAAGTT+TGG - chr1.2:24773536-24773555 None:intergenic 20.0%
!!! ATGAAAATTACACATTTTAC+AGG - chr1.2:24773270-24773289 None:intergenic 20.0%
!!! ATTCCTTTTACAAATGATAA+AGG + chr1.2:24774696-24774715 MS.gene99236:CDS 20.0%
!!! ATTTTAGTTTCTTGAGTTTT+CGG + chr1.2:24773612-24773631 MS.gene99236:intron 20.0%
!!! GATCTCAAAATTTGATTTTT+AGG + chr1.2:24773584-24773603 MS.gene99236:intron 20.0%
!!! TATTGAAGAAAAGTTTTGAA+GGG - chr1.2:24772707-24772726 None:intergenic 20.0%
!!! TCTAGGTTTTAATATCTTTT+TGG - chr1.2:24773479-24773498 None:intergenic 20.0%
!!! TGAAAATTACACATTTTACA+GGG - chr1.2:24773269-24773288 None:intergenic 20.0%
!!! TTCAATTCATTTTAACTTCT+AGG - chr1.2:24773496-24773515 None:intergenic 20.0%
! AAACTTATGGTTGATTGTAA+TGG + chr1.2:24772065-24772084 MS.gene99236:CDS 25.0%
! GAAATAAATAACATTAGGAG+GGG - chr1.2:24772797-24772816 None:intergenic 25.0%
! GATGTTACTCTTAAAGAATT+TGG + chr1.2:24772119-24772138 MS.gene99236:CDS 25.0%
! GATTTATGTTCCACTATTAT+AGG - chr1.2:24772566-24772585 None:intergenic 25.0%
! GCTTTGTGGAAAAATATAAA+GGG + chr1.2:24772366-24772385 MS.gene99236:intron 25.0%
! TACATATTATCGGAGAAATT+TGG + chr1.2:24772262-24772281 MS.gene99236:intron 25.0%
! TCTCCTTTATCATTTGTAAA+AGG - chr1.2:24774702-24774721 None:intergenic 25.0%
! TTATAACCATGATCCAAATA+TGG + chr1.2:24771944-24771963 MS.gene99236:CDS 25.0%
! TTTAGGCCATATAGAAAATA+AGG + chr1.2:24773131-24773150 MS.gene99236:intron 25.0%
!! AAAATGAATTGAAGCCTTAT+CGG + chr1.2:24773503-24773522 MS.gene99236:intron 25.0%
!! AATCTATGTGGTGTTAAAAA+TGG - chr1.2:24773766-24773785 None:intergenic 25.0%
!! AGCTTTGTGGAAAAATATAA+AGG + chr1.2:24772365-24772384 MS.gene99236:intron 25.0%
!! CTTTTCACACTACATATTAT+CGG + chr1.2:24772252-24772271 MS.gene99236:intron 25.0%
!!! GTATTGAAGAAAAGTTTTGA+AGG - chr1.2:24772708-24772727 None:intergenic 25.0%
!!! GTCACATGTTCAATTTTTTA+AGG - chr1.2:24772613-24772632 None:intergenic 25.0%
!!! TTTTAAGGAAGAGAAACAAT+TGG - chr1.2:24772598-24772617 None:intergenic 25.0%
AACATAGCAATGTAACATCA+AGG - chr1.2:24773800-24773819 None:intergenic 30.0%
ACAAATGATAAAGGAGAGAA+AGG + chr1.2:24774705-24774724 MS.gene99236:CDS 30.0%
CACTATTATAGGAAAACATG+GGG - chr1.2:24772555-24772574 None:intergenic 30.0%
CACTTTGAATACTAGAGATT+TGG - chr1.2:24771808-24771827 None:intergenic 30.0%
CCACTATTATAGGAAAACAT+GGG - chr1.2:24772556-24772575 None:intergenic 30.0%
GAAAAGATCAATACTATACC+TGG - chr1.2:24772238-24772257 None:intergenic 30.0%
GAGAAACAATTGGCTATTAT+AGG - chr1.2:24772588-24772607 None:intergenic 30.0%
GTAGAAAGTTCAAATCTATG+TGG - chr1.2:24773778-24773797 None:intergenic 30.0%
GTCAAAATTCAATGCAGATT+TGG - chr1.2:24772984-24773003 None:intergenic 30.0%
GTCCTTTCAATACTTATGTA+AGG - chr1.2:24773299-24773318 None:intergenic 30.0%
TCCACTATTATAGGAAAACA+TGG - chr1.2:24772557-24772576 None:intergenic 30.0%
TGCAGCAATGATTTCAAAAT+TGG - chr1.2:24774253-24774272 None:intergenic 30.0%
TTTATTATCCATTTGCAGGT+AGG + chr1.2:24772021-24772040 MS.gene99236:CDS 30.0%
! AAATCATTGCTGCATACTTT+TGG + chr1.2:24774259-24774278 MS.gene99236:CDS 30.0%
! CTTTTCGAATAACATCAACA+GGG - chr1.2:24771980-24771999 None:intergenic 30.0%
! GCAGATTTTATGGTTATCAA+GGG + chr1.2:24774522-24774541 MS.gene99236:CDS 30.0%
!! AATTGGAGTATTGTTGCAAT+TGG - chr1.2:24774236-24774255 None:intergenic 30.0%
!! GTCCTTACATAAGTATTGAA+AGG + chr1.2:24773294-24773313 MS.gene99236:intron 30.0%
!! GTGTTTTATTATCCATTTGC+AGG + chr1.2:24772017-24772036 MS.gene99236:CDS 30.0%
!! TTGATGGTGTTATCAATTCA+GGG - chr1.2:24771842-24771861 None:intergenic 30.0%
!! TTTGATGGTGTTATCAATTC+AGG - chr1.2:24771843-24771862 None:intergenic 30.0%
!!! AGAGTTTTGGAAAAAAGGAA+AGG - chr1.2:24772831-24772850 None:intergenic 30.0%
!!! GGATTTAGAGATGTTGATTT+TGG + chr1.2:24774600-24774619 MS.gene99236:CDS 30.0%
!!! TAGAGATGTTGATTTTGGTT+GGG + chr1.2:24774605-24774624 MS.gene99236:CDS 30.0%
!!! TTAGAGATGTTGATTTTGGT+TGG + chr1.2:24774604-24774623 MS.gene99236:CDS 30.0%
AAACATGGTTCCTTTGTTGA+CGG - chr1.2:24774133-24774152 None:intergenic 35.0%
AAATTCAATGCATACGCCAA+TGG - chr1.2:24774453-24774472 None:intergenic 35.0%
AACATTAACCAGCCTACTAA+GGG + chr1.2:24774804-24774823 MS.gene99236:CDS 35.0%
AATTTGTGATCACCCTTAGT+AGG - chr1.2:24774819-24774838 None:intergenic 35.0%
ACAACTAGATTCTAACGAAG+AGG + chr1.2:24774302-24774321 MS.gene99236:CDS 35.0%
ACATTTGTATCGAGATCACA+CGG + chr1.2:24772894-24772913 MS.gene99236:intron 35.0%
ACCAATAGAAACAATTCGCT+AGG - chr1.2:24772514-24772533 None:intergenic 35.0%
ACTAAGGGTGATCACAAATT+TGG + chr1.2:24774819-24774838 MS.gene99236:CDS 35.0%
ACTTCTTAGTTCCCTAAATG+AGG + chr1.2:24772729-24772748 MS.gene99236:intron 35.0%
AGTAACCATTTGGAAGTTGT+TGG - chr1.2:24774371-24774390 None:intergenic 35.0%
ATAAATAACATTAGGAGGGG+AGG - chr1.2:24772794-24772813 None:intergenic 35.0%
ATATGCGGTGATTAAACTTG+AGG - chr1.2:24773841-24773860 None:intergenic 35.0%
ATGACCAAGATTCATTACGT+TGG + chr1.2:24771901-24771920 MS.gene99236:CDS 35.0%
ATGGTTGATTGTAATGGTGA+AGG + chr1.2:24772071-24772090 MS.gene99236:CDS 35.0%
CAACTTCCAAATGGTTACTA+TGG + chr1.2:24774372-24774391 MS.gene99236:CDS 35.0%
CACTGAATGCATATACTCTT+TGG - chr1.2:24774502-24774521 None:intergenic 35.0%
CTCTAGTATTCAAAGTGAAG+AGG + chr1.2:24771811-24771830 MS.gene99236:CDS 35.0%
TAAAGAAGCTCATCCAAGAA+AGG - chr1.2:24772167-24772186 None:intergenic 35.0%
TAAATAACATTAGGAGGGGA+GGG - chr1.2:24772793-24772812 None:intergenic 35.0%
TATAATCCCGCAGTAGTTAA+AGG - chr1.2:24774555-24774574 None:intergenic 35.0%
TATACCTGGATAAGTAGCAA+TGG - chr1.2:24772224-24772243 None:intergenic 35.0%
TATTGTTGCAATTGGTGAGT+TGG - chr1.2:24774228-24774247 None:intergenic 35.0%
TCAACCATAAGTTTCCTACT+AGG - chr1.2:24772059-24772078 None:intergenic 35.0%
TGATAAAGGAGAGAAAGGTT+TGG + chr1.2:24774710-24774729 MS.gene99236:CDS 35.0%
TTAATCACATCTGAAGAACC+AGG - chr1.2:24772197-24772216 None:intergenic 35.0%
TTTCGTTGGATCGAAATCAA+AGG - chr1.2:24773008-24773027 None:intergenic 35.0%
! AGTTTGGTAGTATTCCGATA+AGG - chr1.2:24773520-24773539 None:intergenic 35.0%
! CAAAGAGTATATGCATTCAG+TGG + chr1.2:24774500-24774519 MS.gene99236:CDS 35.0%
! CCCATGTTTTCCTATAATAG+TGG + chr1.2:24772553-24772572 MS.gene99236:intron 35.0%
! GCTTTTCGAATAACATCAAC+AGG - chr1.2:24771981-24772000 None:intergenic 35.0%
! GGCAGATTTTATGGTTATCA+AGG + chr1.2:24774521-24774540 MS.gene99236:CDS 35.0%
!! ACTTGAGTTGGCAAAAAAAC+TGG - chr1.2:24774741-24774760 None:intergenic 35.0%
!! AGCGAATTGTTTCTATTGGT+TGG + chr1.2:24772514-24772533 MS.gene99236:intron 35.0%
!!! AGAGATGTTGATTTTGGTTG+GGG + chr1.2:24774606-24774625 MS.gene99236:CDS 35.0%
!!! AGTTTTGTTTCATGAGGTGT+GGG - chr1.2:24771867-24771886 None:intergenic 35.0%
!!! CTTCAATTTTTGAGTGCCTT+AGG + chr1.2:24773991-24774010 MS.gene99236:CDS 35.0%
!!! GAGAGTAGTTTTGTTTCATG+AGG - chr1.2:24771873-24771892 None:intergenic 35.0%
!!! TAGTTTTGTTTCATGAGGTG+TGG - chr1.2:24771868-24771887 None:intergenic 35.0%
!!! TTTTTTGCCAACTCAAGTCA+TGG + chr1.2:24774743-24774762 MS.gene99236:CDS 35.0%
AAACTCCCAAATAAAGCCCT+AGG - chr1.2:24772326-24772345 None:intergenic 40.0%
AACATGGTTCCTTTGTTGAC+GGG - chr1.2:24774132-24774151 None:intergenic 40.0%
AACTCCCAAATAAAGCCCTA+GGG - chr1.2:24772325-24772344 None:intergenic 40.0%
AATTCATTTCTAACCCGTGC+AGG + chr1.2:24773894-24773913 MS.gene99236:intron 40.0%
ACAAATGTTCACCTACGCTT+TGG - chr1.2:24772882-24772901 None:intergenic 40.0%
ACCATGATCCAAATATGGCA+AGG + chr1.2:24771949-24771968 MS.gene99236:CDS 40.0%
AGCTCATCCAAGAAAGGAAA+TGG - chr1.2:24772161-24772180 None:intergenic 40.0%
AGGTCCTAGTAGGAAACTTA+TGG + chr1.2:24772052-24772071 MS.gene99236:CDS 40.0%
ATATGAAAGACAGCTTGACC+AGG - chr1.2:24774678-24774697 None:intergenic 40.0%
ATCCATTTGCAGGTAGGTTA+AGG + chr1.2:24772027-24772046 MS.gene99236:CDS 40.0%
ATTTCGATCCAACGAAACGA+TGG + chr1.2:24773011-24773030 MS.gene99236:intron 40.0%
CAACATTAACCAGCCTACTA+AGG + chr1.2:24774803-24774822 MS.gene99236:CDS 40.0%
CAATGAGTACATGTCACTCT+TGG - chr1.2:24774093-24774112 None:intergenic 40.0%
CAGAGAGCTTCTTAATGCAA+GGG + chr1.2:24774062-24774081 MS.gene99236:CDS 40.0%
CATACATTGAGTGATGCATC+AGG + chr1.2:24773964-24773983 MS.gene99236:CDS 40.0%
CTGATGCATCACTCAATGTA+TGG - chr1.2:24773966-24773985 None:intergenic 40.0%
CTTGCCAACGTAATGAATCT+TGG - chr1.2:24771908-24771927 None:intergenic 40.0%
GAAAAAAGGAAAGGAGCAAG+TGG - chr1.2:24772822-24772841 None:intergenic 40.0%
GAGCTTCTTTATGATGTTCC+TGG + chr1.2:24772176-24772195 MS.gene99236:CDS 40.0%
GAGTAAACCTTCAATCTCAC+CGG + chr1.2:24774032-24774051 MS.gene99236:CDS 40.0%
GCGTTACCATAGTAACCATT+TGG - chr1.2:24774381-24774400 None:intergenic 40.0%
GTTGACCAACAACTTCCAAA+TGG + chr1.2:24774363-24774382 MS.gene99236:CDS 40.0%
TCATCCAAGAAAGGAAATGG+AGG - chr1.2:24772158-24772177 None:intergenic 40.0%
TCATCCTCCATTTCCTTTCT+TGG + chr1.2:24772151-24772170 MS.gene99236:CDS 40.0%
TCCATTTGCAGGTAGGTTAA+GGG + chr1.2:24772028-24772047 MS.gene99236:CDS 40.0%
TCCTTGCCATATTTGGATCA+TGG - chr1.2:24771953-24771972 None:intergenic 40.0%
TTTCGATCCAACGAAACGAT+GGG + chr1.2:24773012-24773031 MS.gene99236:intron 40.0%
! CTTTATTTGGGAGTTTGGAG+AGG + chr1.2:24772330-24772349 MS.gene99236:intron 40.0%
! GCATTCAGTGGCAGATTTTA+TGG + chr1.2:24774512-24774531 MS.gene99236:CDS 40.0%
!! AACAGATTTGCTACGTGCAT+CGG - chr1.2:24774346-24774365 None:intergenic 40.0%
!! GCCTAGCGAATTGTTTCTAT+TGG + chr1.2:24772510-24772529 MS.gene99236:intron 40.0%
!! TAGGGCTTTATTTGGGAGTT+TGG + chr1.2:24772325-24772344 MS.gene99236:intron 40.0%
!!! AGGGGAGAGTTTTGGAAAAA+AGG - chr1.2:24772836-24772855 None:intergenic 40.0%
!!! GAGATGTTGATTTTGGTTGG+GGG + chr1.2:24774607-24774626 MS.gene99236:CDS 40.0%
AACCTCTCCATGACTTGAGT+TGG - chr1.2:24774753-24774772 None:intergenic 45.0%
AACTCAAGTCATGGAGAGGT+TGG + chr1.2:24774752-24774771 MS.gene99236:CDS 45.0%
AATCCCGCAGTAGTTAAAGG+AGG - chr1.2:24774552-24774571 None:intergenic 45.0%
ATTTGGGAGTTTGGAGAGGA+GGG + chr1.2:24772334-24772353 MS.gene99236:intron 45.0%
CAAACTTGCAACCAAAGCGT+AGG + chr1.2:24772868-24772887 MS.gene99236:intron 45.0%
CAATCAACCCATCGTTTCGT+TGG - chr1.2:24773022-24773041 None:intergenic 45.0%
CATGTCGTCTAAGGGAAACA+TGG - chr1.2:24774148-24774167 None:intergenic 45.0%
CATGTTTCCCTTAGACGACA+TGG + chr1.2:24774146-24774165 MS.gene99236:CDS 45.0%
CTACGCTTTGGTTGCAAGTT+TGG - chr1.2:24772870-24772889 None:intergenic 45.0%
CTTTGGTTGCAAGTTTGGAG+GGG - chr1.2:24772865-24772884 None:intergenic 45.0%
GCAGAGAGCTTCTTAATGCA+AGG + chr1.2:24774061-24774080 MS.gene99236:CDS 45.0%
GCTTTGGTTGCAAGTTTGGA+GGG - chr1.2:24772866-24772885 None:intergenic 45.0%
GGGTCTTTCCTTGCCATATT+TGG - chr1.2:24771960-24771979 None:intergenic 45.0%
GTTCACCGCACTTTCTTCAT+TGG + chr1.2:24774168-24774187 MS.gene99236:CDS 45.0%
GTTTATGGAGGACCAGCTAA+AGG + chr1.2:24774636-24774655 MS.gene99236:CDS 45.0%
TATTTGGGAGTTTGGAGAGG+AGG + chr1.2:24772333-24772352 MS.gene99236:intron 45.0%
TCCCTTAACCTACCTGCAAA+TGG - chr1.2:24772032-24772051 None:intergenic 45.0%
TCGTCCTCCTTTAACTACTG+CGG + chr1.2:24774545-24774564 MS.gene99236:CDS 45.0%
TGAGTACATGTCACTCTTGG+TGG - chr1.2:24774090-24774109 None:intergenic 45.0%
TGGGACCAATGAAGAAAGTG+CGG - chr1.2:24774176-24774195 None:intergenic 45.0%
TTTGCAGGTAGGTTAAGGGA+AGG + chr1.2:24772032-24772051 MS.gene99236:CDS 45.0%
TTTGGTTGCAAGTTTGGAGG+GGG - chr1.2:24772864-24772883 None:intergenic 45.0%
! CACTCCATTGCTACTTATCC+AGG + chr1.2:24772217-24772236 MS.gene99236:CDS 45.0%
! GGAGAGGTTGGTTAAAGAGT+TGG + chr1.2:24774764-24774783 MS.gene99236:CDS 45.0%
! GTTGCAATTGGTGAGTTGGA+AGG - chr1.2:24774224-24774243 None:intergenic 45.0%
!! CTGTCCCTAGGGCTTTATTT+GGG + chr1.2:24772318-24772337 MS.gene99236:intron 45.0%
!! TCATGAGGTGTGGGTTTTGA+TGG - chr1.2:24771858-24771877 None:intergenic 45.0%
!!! AAATAAAATATATTTTTACA+AGG + chr1.2:24773423-24773442 MS.gene99236:intron 5.0%
!!! ATTTCATTTATATATTTTTT+TGG - chr1.2:24772960-24772979 None:intergenic 5.0%
!!! TATTTTTAATATTAAGAATA+CGG + chr1.2:24772464-24772483 MS.gene99236:intron 5.0%
!!! TATTTTTTTCATTTAATTTT+AGG + chr1.2:24773114-24773133 MS.gene99236:intron 5.0%
AACCTTCAATCTCACCGGTG+TGG + chr1.2:24774037-24774056 MS.gene99236:CDS 50.0%
ACACTTTCACGCTGTCCCTA+GGG + chr1.2:24772307-24772326 MS.gene99236:intron 50.0%
AGAGCGAATTGCAGCAACCT+CGG - chr1.2:24774199-24774218 None:intergenic 50.0%
AGCGTTATTCACAGCGAAGC+AGG - chr1.2:24772635-24772654 None:intergenic 50.0%
ATGGAGGACCAGCTAAAGGA+GGG + chr1.2:24774640-24774659 MS.gene99236:CDS 50.0%
CAGGTAACACGCTTCAAGTG+TGG + chr1.2:24773913-24773932 MS.gene99236:intron 50.0%
CCTTAGGAGAGATATCTCGC+GGG + chr1.2:24774007-24774026 MS.gene99236:CDS 50.0%
CGCTTTAGCATTTCCCGTTG+CGG + chr1.2:24774398-24774417 MS.gene99236:CDS 50.0%
CGCTTTGGTTGCAAGTTTGG+AGG - chr1.2:24772867-24772886 None:intergenic 50.0%
CGGTGAACCATGTCGTCTAA+GGG - chr1.2:24774156-24774175 None:intergenic 50.0%
CGTCCTCCTTTAACTACTGC+GGG + chr1.2:24774546-24774565 MS.gene99236:CDS 50.0%
CTTGAAGCGTGTTACCTGCA+CGG - chr1.2:24773911-24773930 None:intergenic 50.0%
GATCCAGAACCCGTCAACAA+AGG + chr1.2:24774120-24774139 MS.gene99236:CDS 50.0%
GCCTTAGGAGAGATATCTCG+CGG + chr1.2:24774006-24774025 MS.gene99236:CDS 50.0%
GGTTAAGGGAAGGTCCTAGT+AGG + chr1.2:24772042-24772061 MS.gene99236:CDS 50.0%
GGTTGGGGGAAAGCTGTTTA+TGG + chr1.2:24774621-24774640 MS.gene99236:CDS 50.0%
GTAACACGCTTCAAGTGTGG+TGG + chr1.2:24773916-24773935 MS.gene99236:CDS 50.0%
GTGTCTGATGTTACACGTGC+TGG + chr1.2:24774579-24774598 MS.gene99236:CDS 50.0%
TATGGAGGACCAGCTAAAGG+AGG + chr1.2:24774639-24774658 MS.gene99236:CDS 50.0%
TCCTGCAGTTGTAACCGCAA+CGG - chr1.2:24774415-24774434 None:intergenic 50.0%
TGCCAACTCAAGTCATGGAG+AGG + chr1.2:24774748-24774767 MS.gene99236:CDS 50.0%
TGGGGGAAAGCTGTTTATGG+AGG + chr1.2:24774624-24774643 MS.gene99236:CDS 50.0%
TGTGATCACCCTTAGTAGGC+TGG - chr1.2:24774815-24774834 None:intergenic 50.0%
TTGAAGCGTGTTACCTGCAC+GGG - chr1.2:24773910-24773929 None:intergenic 50.0%
TTGCAAGTTTGGAGGGGGAA+GGG - chr1.2:24772859-24772878 None:intergenic 50.0%
TTTCTTCATTGGTCCCACCG+AGG + chr1.2:24774179-24774198 MS.gene99236:CDS 50.0%
TTTGGGAGTTTGGAGAGGAG+GGG + chr1.2:24772335-24772354 MS.gene99236:intron 50.0%
!! GCTGTCCCTAGGGCTTTATT+TGG + chr1.2:24772317-24772336 MS.gene99236:intron 50.0%
!! GTTCCTTTGTTGACGGGTTC+TGG - chr1.2:24774126-24774145 None:intergenic 50.0%
AAGTTTGGAGGGGGAAGGGA+GGG - chr1.2:24772855-24772874 None:intergenic 55.0%
AGAAGCTCTCTGCTCCACAC+CGG - chr1.2:24774054-24774073 None:intergenic 55.0%
CAATTCGCTAGGCATTCGCG+AGG - chr1.2:24772503-24772522 None:intergenic 55.0%
CACACTTTCACGCTGTCCCT+AGG + chr1.2:24772306-24772325 MS.gene99236:intron 55.0%
CCCGCGAGATATCTCTCCTA+AGG - chr1.2:24774010-24774029 None:intergenic 55.0%
CCTGCAGTTGTAACCGCAAC+GGG - chr1.2:24774414-24774433 None:intergenic 55.0%
CGAATTGCAGCAACCTCGGT+GGG - chr1.2:24774195-24774214 None:intergenic 55.0%
CTCCACACCGGTGAGATTGA+AGG - chr1.2:24774042-24774061 None:intergenic 55.0%
GAGGGGAAGAGAGAGCTTTG+TGG + chr1.2:24772352-24772371 MS.gene99236:intron 55.0%
GCGGTGAACCATGTCGTCTA+AGG - chr1.2:24774157-24774176 None:intergenic 55.0%
GTTGCAAGTTTGGAGGGGGA+AGG - chr1.2:24772860-24772879 None:intergenic 55.0%
TGGAGGACCAGCTAAAGGAG+GGG + chr1.2:24774641-24774660 MS.gene99236:CDS 55.0%
! TTAGGAGGGGAGGGCTTTAG+AGG - chr1.2:24772784-24772803 None:intergenic 55.0%
!! TAGGAGGGGAGGGCTTTAGA+GGG - chr1.2:24772783-24772802 None:intergenic 55.0%
AGTTTGGAGGGGGAAGGGAG+GGG - chr1.2:24772854-24772873 None:intergenic 60.0%
CAAGTTTGGAGGGGGAAGGG+AGG - chr1.2:24772856-24772875 None:intergenic 60.0%
CCCGTTGCGGTTACAACTGC+AGG + chr1.2:24774411-24774430 MS.gene99236:CDS 60.0%
GCACCATCCCCTCCTTTAGC+TGG - chr1.2:24774651-24774670 None:intergenic 60.0%
GCGAATTGCAGCAACCTCGG+TGG - chr1.2:24774196-24774215 None:intergenic 60.0%
GGACCAGCTAAAGGAGGGGA+TGG + chr1.2:24774645-24774664 MS.gene99236:CDS 60.0%
! GGGAAGGGAGGGGAGAGTTT+TGG - chr1.2:24772844-24772863 None:intergenic 60.0%
GGAGGGGATGGTGCTCTTCC+TGG + chr1.2:24774657-24774676 MS.gene99236:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 24771801 24774863 24771801 ID=MS.gene99236
chr1.2 mRNA 24771801 24774863 24771801 ID=MS.gene99236.t1;Parent=MS.gene99236
chr1.2 exon 24771801 24772238 24771801 ID=MS.gene99236.t1.exon1;Parent=MS.gene99236.t1
chr1.2 CDS 24771801 24772238 24771801 ID=cds.MS.gene99236.t1;Parent=MS.gene99236.t1
chr1.2 exon 24773916 24774863 24773916 ID=MS.gene99236.t1.exon2;Parent=MS.gene99236.t1
chr1.2 CDS 24773916 24774863 24773916 ID=cds.MS.gene99236.t1;Parent=MS.gene99236.t1
Gene Sequence

>MS.gene99236

ATGGCCAAATCTCTAGTATTCAAAGTGAAGAGGCGTGACCCTGAATTGATAACACCATCAAAACCCACACCTCATGAAACAAAACTACTCTCAGACATTGATGACCAAGATTCATTACGTTGGCAAGTTCCTTTGATACAATTTTATAACCATGATCCAAATATGGCAAGGAAAGACCCTGTTGATGTTATTCGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGGTTAAGGGAAGGTCCTAGTAGGAAACTTATGGTTGATTGTAATGGTGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATGTTACTCTTAAAGAATTTGGTGATGCTCTTCATCCTCCATTTCCTTTCTTGGATGAGCTTCTTTATGATGTTCCTGGTTCTTCAGATGTGATTAACACTCCATTGCTACTTATCCAGGTATAGTATTGATCTTTTCACACTACATATTATCGGAGAAATTTGGATTCTAGTCAGTCATGATTTTCACACTTTCACGCTGTCCCTAGGGCTTTATTTGGGAGTTTGGAGAGGAGGGGAAGAGAGAGCTTTGTGGAAAAATATAAAGGGAAATAGAGAAATCTCTCACATTTTTTCAAAAGAATATTTTTTTAGAGAAAGAGAAACTAATGCTTATAATTCATATATTTTTAATATTAAGAATACGGAAATGCTTCTTCTCCTCGCGAATGCCTAGCGAATTGTTTCTATTGGTTGGTCAAACTTCAACACTCCCCATGTTTTCCTATAATAGTGGAACATAAATCCTATAATAGCCAATTGTTTCTCTTCCTTAAAAAATTGAACATGTGACCTGCTTCGCTGTGAATAACGCTGCTCTTAAGAATATTATAACATAGATTTAATTTGAAGAATTAATATAAACCCTTCAAAACTTTTCTTCAATACAACTTCTTAGTTCCCTAAATGAGGATAATTTTGTATTATGAAGAAAAATTAACCCTCTAAAGCCCTCCCCTCCTAATGTTATTTATTTCTTCCACTTGCTCCTTTCCTTTTTTCCAAAACTCTCCCCTCCCTTCCCCCTCCAAACTTGCAACCAAAGCGTAGGTGAACATTTGTATCGAGATCACACGGTTCATATTTCAAAATCAAAATAAATAAAAAATCAACTGTTCCAAAAAAATATATAAATGAAATTCCAAATCTGCATTGAATTTTGACTCCTTTGATTTCGATCCAACGAAACGATGGGTTGATTGCGTGATTGTATGAAAATCGTGACAAAGTCATCTAATTTTTATTTTTTTATTTGATTTTAAGTCTCTAAAATATATTTTTTTCATTTAATTTTAGGCCATATAGAAAATAAGGTTTTTTCCTAAAAAAAAAAAAAAAAGGTTTAAATATAGTTTTGTTACTTGTAAGTATACAATTTTTTTTGTTTGTTTTAGTCATTAGAAGATTTGTTAATGTCAAAATTAGTCCCTGTAAAATGTGTAATTTTCATTTTAGTCCTTACATAAGTATTGAAAGGACTGAAAACTAAACATTTCACATTTTACATGATCAATTCCAACATAAAATATTTTATTAGGACTGAATTCTAATGTAAAGCAATTTATTTTATCTTTTCAAGGTCTAAATAAAATATATTTTTACAAGGTTGAAAAATAGAAAGTATTATATTTGTATGCCAAAAAGATATTAAAACCTAGAAGTTAAAATGAATTGAAGCCTTATCGGAATACTACCAAACTTTTAAATACTCTTGATGTGTTATCATAGAAGTTGTTGCCAAAATGATCTCAAAATTTGATTTTTAGGTCTATATTTTAGTTTCTTGAGTTTTCGGAGCTTAAATCTTTTATGATTAATCCTTCACTTATTAAAATAATTTAAACTTTTTGTGAGGAGTTGTTTATAATGTTCTAAACATTCCTTAAAAAAAATGATTCAAATATATGTGGTACCAATTAATGACCATTTTTAACACCACATAGATTTGAACTTTCTACCTTGATGTTACATTGCTATGTTTTTCAAACTAAGCTAGAACCTCAAGTTTAATCACCGCATATCAATTGATTTTAATAAATCTTTAATCATTCATTAATTCATTTCTAACCCGTGCAGGTAACACGCTTCAAGTGTGGTGGTTTCATCTTTGCTCTTCGTATGAACCATACATTGAGTGATGCATCAGGTATGCTTCAATTTTTGAGTGCCTTAGGAGAGATATCTCGCGGGATGAGTAAACCTTCAATCTCACCGGTGTGGAGCAGAGAGCTTCTTAATGCAAGGGATCCACCAAGAGTGACATGTACTCATTGTGAATACGATCCAGAACCCGTCAACAAAGGAACCATGTTTCCCTTAGACGACATGGTTCACCGCACTTTCTTCATTGGTCCCACCGAGGTTGCTGCAATTCGCTCTCTCCTTCCAACTCACCAATTGCAACAATACTCCAATTTTGAAATCATTGCTGCATACTTTTGGCGTTGTCGTACAATAGCATTACAACTAGATTCTAACGAAGAGGTTCGCATAATCTGCATAGCCGATGCACGTAGCAAATCTGTTGACCAACAACTTCCAAATGGTTACTATGGTAACGCTTTAGCATTTCCCGTTGCGGTTACAACTGCAGGAAAACTTATTGAAAATCCATTGGCGTATGCATTGAATTTAGTTAAGAAAGCAAAAACTATTGTCACCAAAGAGTATATGCATTCAGTGGCAGATTTTATGGTTATCAAGGGTCGTCCTCCTTTAACTACTGCGGGATTATATTTAGTGTCTGATGTTACACGTGCTGGATTTAGAGATGTTGATTTTGGTTGGGGGAAAGCTGTTTATGGAGGACCAGCTAAAGGAGGGGATGGTGCTCTTCCTGGTCAAGCTGTCTTTCATATTCCTTTTACAAATGATAAAGGAGAGAAAGGTTTGGTTATACCAGTTTTTTTGCCAACTCAAGTCATGGAGAGGTTGGTTAAAGAGTTGGATAGTGTTCTTAAGAACAACATTAACCAGCCTACTAAGGGTGATCACAAATTTGGCTTCATTAAATCTTCGTTGTAG
Protein sequence

>MS.gene99236.t1

MAKSLVFKVKRRDPELITPSKPTPHETKLLSDIDDQDSLRWQVPLIQFYNHDPNMARKDPVDVIRKALAKTLVFYYPFAGRLREGPSRKLMVDCNGEGVLFIEADADVTLKEFGDALHPPFPFLDELLYDVPGSSDVINTPLLLIQVTRFKCGGFIFALRMNHTLSDASGMLQFLSALGEISRGMSKPSISPVWSRELLNARDPPRVTCTHCEYDPEPVNKGTMFPLDDMVHRTFFIGPTEVAAIRSLLPTHQLQQYSNFEIIAAYFWRCRTIALQLDSNEEVRIICIADARSKSVDQQLPNGYYGNALAFPVAVTTAGKLIENPLAYALNLVKKAKTIVTKEYMHSVADFMVIKGRPPLTTAGLYLVSDVTRAGFRDVDFGWGKAVYGGPAKGGDGALPGQAVFHIPFTNDKGEKGLVIPVFLPTQVMERLVKELDSVLKNNINQPTKGDHKFGFIKSSL