AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99515

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99515.t1 MTR_1g040175 86.117 461 60 2 1 460 1 458 0.0 817
MS.gene99515.t1 MTR_1g040320 87.419 461 21 2 1 460 1 425 0.0 805
MS.gene99515.t1 MTR_1g040215 76.790 461 106 1 1 460 1 461 0.0 741
MS.gene99515.t1 MTR_1g040195 72.000 450 101 3 1 450 1 425 0.0 662
MS.gene99515.t1 MTR_1g040315 65.556 450 111 4 1 450 1 406 0.0 581
MS.gene99515.t1 MTR_7g114550 54.945 455 198 6 2 453 3 453 0.0 525
MS.gene99515.t1 MTR_7g114560 55.705 447 191 6 5 448 6 448 0.0 520
MS.gene99515.t1 MTR_7g114520 56.000 450 187 7 5 448 7 451 0.0 518
MS.gene99515.t1 MTR_5g076700 54.148 458 202 6 5 458 6 459 4.99e-180 511
MS.gene99515.t1 MTR_7g114470 54.343 449 196 6 5 448 7 451 1.59e-176 503
MS.gene99515.t1 MTR_1g081660 57.175 439 183 5 4 440 13 448 2.50e-173 494
MS.gene99515.t1 MTR_7g114490 54.630 432 188 5 5 432 6 433 1.50e-171 491
MS.gene99515.t1 MTR_0042s0190 73.737 297 77 1 165 460 1 297 2.63e-162 460
MS.gene99515.t1 MTR_0042s0190 74.569 232 58 1 230 460 1 232 1.09e-122 357
MS.gene99515.t1 MTR_7g114510 51.792 307 138 6 147 448 8 309 3.13e-103 310
MS.gene99515.t1 MTR_0502s0010 57.613 243 95 4 100 338 1 239 6.53e-94 284
MS.gene99515.t1 MTR_3g035000 60.811 222 60 3 199 419 43 238 3.95e-84 261
MS.gene99515.t1 MTR_0042s0230 82.993 147 25 0 1 147 1 147 1.29e-83 254
MS.gene99515.t1 MTR_7g114420 44.792 288 124 6 165 448 1 257 5.31e-78 244
MS.gene99515.t1 MTR_3g098980 32.401 429 271 10 9 432 14 428 3.06e-76 245
MS.gene99515.t1 MTR_8g096530 32.339 436 273 12 1 430 1 420 7.75e-74 239
MS.gene99515.t1 MTR_7g027175 33.854 384 242 8 13 391 20 396 2.06e-73 238
MS.gene99515.t1 MTR_8g039950 81.250 128 24 0 1 128 1 128 4.27e-70 219
MS.gene99515.t1 MTR_7g114500 59.333 150 61 0 2 151 3 152 1.80e-62 200
MS.gene99515.t1 MTR_3g051790 61.644 146 56 0 2 147 6 151 5.71e-58 189
MS.gene99515.t1 MTR_3g112180 33.419 389 231 12 17 391 20 394 1.23e-56 194
MS.gene99515.t1 MTR_1g053315 30.023 443 286 13 17 440 26 463 5.11e-54 187
MS.gene99515.t1 MTR_4g007540 30.047 426 272 12 9 420 3 416 1.32e-52 183
MS.gene99515.t1 MTR_8g075610 29.977 427 253 11 16 420 1 403 8.61e-52 181
MS.gene99515.t1 MTR_4g055170 28.200 461 310 10 3 446 6 462 1.06e-51 181
MS.gene99515.t1 MTR_2g105330 28.864 440 287 12 9 436 3 428 5.82e-50 176
MS.gene99515.t1 MTR_2g064495 28.864 440 287 12 9 436 3 428 2.85e-49 174
MS.gene99515.t1 MTR_3g071320 30.303 396 258 10 9 391 5 395 5.11e-47 168
MS.gene99515.t1 MTR_7g110610 29.327 416 225 13 5 411 1 356 1.94e-43 157
MS.gene99515.t1 MTR_7g084940 28.173 394 268 9 5 391 12 397 9.34e-43 157
MS.gene99515.t1 MTR_3g465990 29.032 465 279 17 9 439 10 457 2.39e-41 153
MS.gene99515.t1 MTR_7g086000 30.296 406 236 16 17 385 12 407 6.82e-41 152
MS.gene99515.t1 MTR_7g084910 27.960 397 245 11 8 391 16 384 2.21e-40 150
MS.gene99515.t1 MTR_3g465930 27.862 463 295 14 7 439 8 461 3.53e-40 150
MS.gene99515.t1 MTR_7g085990 27.897 466 280 18 9 434 2 451 1.58e-39 148
MS.gene99515.t1 MTR_7g085930 27.830 424 258 15 9 392 6 421 2.24e-39 148
MS.gene99515.t1 MTR_7g056087 28.315 445 271 16 16 434 11 433 3.24e-39 147
MS.gene99515.t1 MTR_6g053810 26.022 465 309 13 3 439 5 462 2.96e-38 145
MS.gene99515.t1 MTR_3g465980 28.416 461 287 16 9 439 10 457 8.22e-38 144
MS.gene99515.t1 MTR_3g465910 30.145 345 220 9 7 337 8 345 8.74e-38 145
MS.gene99515.t1 MTR_2g015430 30.102 392 252 13 9 387 2 384 1.10e-37 143
MS.gene99515.t1 MTR_4g127650 27.778 414 262 12 8 392 9 414 1.20e-37 143
MS.gene99515.t1 MTR_3g026950 26.225 408 261 10 17 392 10 409 2.39e-35 136
MS.gene99515.t1 MTR_4g097090 27.419 434 273 14 17 420 17 438 5.87e-34 133
MS.gene99515.t1 MTR_3g465880 27.802 464 297 15 7 439 8 464 3.19e-33 131
MS.gene99515.t1 MTR_4g088765 78.082 73 16 0 1 73 1 73 3.31e-33 122
MS.gene99515.t1 MTR_3g465950 30.233 344 224 9 7 337 8 348 8.55e-33 129
MS.gene99515.t1 MTR_4g013050 26.316 437 251 16 17 430 12 400 2.47e-31 124
MS.gene99515.t1 MTR_3g049150 26.485 404 257 12 17 390 10 403 1.30e-30 123
MS.gene99515.t1 MTR_5g090830 26.683 401 232 14 61 427 63 435 2.46e-29 120
MS.gene99515.t1 MTR_5g090810 26.434 401 233 14 61 427 63 435 1.46e-28 117
MS.gene99515.t1 MTR_8g467590 25.485 412 270 15 15 405 9 404 1.75e-25 108
MS.gene99515.t1 MTR_5g090880 25.964 389 228 14 61 414 64 427 1.85e-25 108
MS.gene99515.t1 MTR_3g465870 29.010 293 187 10 5 282 8 294 2.19e-25 108
MS.gene99515.t1 MTR_1g094790 26.573 429 247 19 15 404 9 408 1.00e-24 106
MS.gene99515.t1 MTR_3g450710 26.107 429 236 17 64 446 65 458 2.90e-24 105
MS.gene99515.t1 MTR_3g070170 26.168 428 245 15 53 432 55 459 1.30e-23 103
MS.gene99515.t1 MTR_5g084870 24.505 404 240 16 15 388 9 377 2.04e-23 102
MS.gene99515.t1 MTR_3g450690 25.118 422 244 15 64 441 72 465 3.04e-23 102
MS.gene99515.t1 MTR_8g467580 25.366 410 273 15 15 405 9 404 6.34e-23 101
MS.gene99515.t1 MTR_4g094842 26.247 381 238 14 48 399 46 412 2.51e-22 99.4
MS.gene99515.t1 MTR_8g041770 26.575 365 234 14 49 395 49 397 1.05e-21 97.4
MS.gene99515.t1 MTR_4g094828 24.570 407 266 14 51 432 49 439 1.21e-21 97.4
MS.gene99515.t1 MTR_3g086020 24.372 398 240 17 15 390 11 369 2.70e-21 96.3
MS.gene99515.t1 MTR_4g022290 23.823 361 222 13 59 387 55 394 6.78e-21 95.1
MS.gene99515.t1 MTR_5g084850 22.704 392 261 13 15 388 9 376 1.39e-19 91.3
MS.gene99515.t1 MTR_8g079710 24.874 398 258 15 59 432 54 434 1.87e-19 90.9
MS.gene99515.t1 MTR_8g079970 23.018 391 268 12 15 388 9 383 3.31e-19 90.1
MS.gene99515.t1 MTR_3g101930 26.722 363 222 15 64 397 70 417 3.84e-19 90.1
MS.gene99515.t1 MTR_5g026320 26.804 388 246 16 22 389 33 402 4.20e-19 89.7
MS.gene99515.t1 MTR_7g026860 26.238 404 261 17 48 432 42 427 1.50e-18 88.2
MS.gene99515.t1 MTR_4g094832 26.257 358 222 15 94 432 85 419 2.21e-18 87.4
MS.gene99515.t1 MTR_8g079950 32.778 180 103 6 15 187 9 177 3.78e-18 87.0
MS.gene99515.t1 MTR_4g022720 24.603 378 234 15 51 399 44 399 8.45e-18 85.9
MS.gene99515.t1 MTR_4g094838 22.703 370 237 12 53 399 53 396 1.15e-17 85.5
MS.gene99515.t1 MTR_4g022800 24.874 398 256 15 23 395 25 404 1.16e-17 85.5
MS.gene99515.t1 MTR_3g101900 24.819 415 253 18 61 445 63 448 1.49e-17 85.1
MS.gene99515.t1 MTR_3g102020 25.683 366 237 16 61 402 66 420 7.88e-17 82.8
MS.gene99515.t1 MTR_3g102010 26.330 376 226 17 61 402 62 420 2.31e-16 81.6
MS.gene99515.t1 MTR_5g084900 22.054 331 213 12 64 388 9 300 6.87e-16 79.3
MS.gene99515.t1 MTR_4g022825 25.495 404 251 17 57 432 48 429 7.66e-16 79.7
MS.gene99515.t1 MTR_7g114417 65.385 52 17 1 89 139 29 80 1.27e-15 72.4
MS.gene99515.t1 MTR_6g045553 22.616 451 245 18 58 441 60 473 8.27e-15 76.6
MS.gene99515.t1 MTR_4g022760 28.102 274 179 9 64 323 55 324 1.80e-14 75.5
MS.gene99515.t1 MTR_3g051820 46.237 93 46 3 367 458 1 90 3.57e-14 68.6
MS.gene99515.t1 MTR_2g036780 23.410 393 237 11 72 437 74 429 2.96e-13 72.0
MS.gene99515.t1 MTR_2g007880 22.749 422 256 20 6 389 14 403 2.22e-12 69.3
MS.gene99515.t1 MTR_3g049140 31.333 150 84 5 257 390 54 200 7.81e-12 65.9
MS.gene99515.t1 MTR_4g007460 32.143 140 85 4 79 218 2 131 1.02e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99515.t1 AT5G17540 54.721 466 200 8 1 460 1 461 8.29e-176 501
MS.gene99515.t1 AT3G03480 52.381 441 199 8 5 440 15 449 4.96e-155 447
MS.gene99515.t1 AT5G41040 33.907 407 246 11 7 404 15 407 8.15e-79 252
MS.gene99515.t1 AT5G41040 33.907 407 246 11 7 404 31 423 1.07e-78 252
MS.gene99515.t1 AT5G63560 32.723 437 268 12 1 430 1 418 6.63e-78 249
MS.gene99515.t1 AT3G48720 29.885 435 287 10 1 430 1 422 1.59e-73 238
MS.gene99515.t1 AT1G27620 29.797 443 288 10 10 446 10 435 3.99e-54 187
MS.gene99515.t1 AT5G48930 29.821 446 279 13 8 436 2 430 3.21e-52 182
MS.gene99515.t1 AT3G62160 32.178 404 236 13 5 394 1 380 6.47e-50 176
MS.gene99515.t1 AT1G03390 31.726 394 244 12 14 391 27 411 1.01e-48 173
MS.gene99515.t1 AT1G28680 27.716 451 297 13 13 444 8 448 1.39e-48 172
MS.gene99515.t1 AT5G57840 28.019 414 261 12 8 397 2 402 4.66e-47 168
MS.gene99515.t1 AT2G19070 29.095 409 256 11 12 392 7 409 2.95e-46 166
MS.gene99515.t1 AT2G40230 30.892 437 254 17 9 421 5 417 7.55e-46 165
MS.gene99515.t1 AT1G28680 28.997 369 242 10 13 368 8 369 2.01e-44 160
MS.gene99515.t1 AT1G65450 28.571 455 283 16 7 440 16 449 1.34e-42 156
MS.gene99515.t1 AT3G47170 27.215 474 294 16 4 440 7 466 2.25e-39 148
MS.gene99515.t1 AT2G23510 28.266 467 286 16 1 439 5 450 1.86e-38 145
MS.gene99515.t1 AT5G07080 28.856 402 251 11 9 387 14 403 3.81e-38 144
MS.gene99515.t1 AT2G25150 27.273 451 301 14 9 441 13 454 2.31e-36 139
MS.gene99515.t1 AT1G32910 28.642 405 248 14 62 441 63 451 2.35e-34 134
MS.gene99515.t1 AT1G78990 28.165 387 231 13 62 431 65 421 2.27e-33 131
MS.gene99515.t1 AT1G31490 28.075 374 224 15 38 389 36 386 4.48e-29 119
MS.gene99515.t1 AT4G31910 28.354 395 237 15 25 384 24 407 9.05e-26 109
MS.gene99515.t1 AT1G24420 26.633 398 240 15 15 388 11 380 7.97e-24 103
MS.gene99515.t1 AT1G24420 26.633 398 240 15 15 388 11 380 7.97e-24 103
MS.gene99515.t1 AT5G16410 25.779 353 219 13 62 390 89 422 1.23e-22 100
MS.gene99515.t1 AT3G26040 25.063 395 257 15 15 390 9 383 2.06e-22 99.8
MS.gene99515.t1 AT1G24430 23.951 405 249 15 15 393 11 382 3.51e-22 99.0
MS.gene99515.t1 AT5G38130 26.055 403 247 17 23 399 48 425 1.39e-21 97.4
MS.gene99515.t1 AT5G23970 25.183 409 248 14 8 391 2 377 2.42e-21 96.3
MS.gene99515.t1 AT5G07850 26.316 361 236 14 64 402 70 422 1.07e-20 94.7
MS.gene99515.t1 AT5G07860 25.377 398 245 12 64 437 72 441 4.86e-20 92.8
MS.gene99515.t1 AT3G50280 24.860 358 238 13 64 399 59 407 8.65e-20 92.0
MS.gene99515.t1 AT5G42830 26.379 417 256 15 21 402 18 418 2.16e-19 90.9
MS.gene99515.t1 AT3G50270 25.067 371 223 13 64 399 62 412 2.62e-19 90.5
MS.gene99515.t1 AT5G47980 26.247 461 281 20 15 447 11 440 3.08e-18 87.0
MS.gene99515.t1 AT5G67160 24.615 455 283 16 13 437 2 426 3.30e-18 87.0
MS.gene99515.t1 AT1G65450 25.166 302 189 12 158 440 2 285 3.52e-17 82.0
MS.gene99515.t1 AT2G39980 24.439 401 250 11 53 402 51 449 6.22e-16 80.5
MS.gene99515.t1 AT3G30280 22.439 410 264 16 7 388 4 387 4.50e-15 77.4
MS.gene99515.t1 AT1G65450 34.014 147 92 4 7 151 16 159 3.68e-12 65.1
MS.gene99515.t1 AT3G23840 24.399 291 172 9 80 353 81 340 5.77e-12 67.8
MS.gene99515.t1 AT5G47950 24.359 312 197 12 7 309 4 285 7.43e-12 67.4

Find 104 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCCATTTCCTTTCT+TGG 0.075005 1.3:-23140633 MS.gene99515:CDS
GGATCTTTCCCTGCCATATT+TGG 0.126530 1.3:+23140827 None:intergenic
TCTCCTGTATCATTTGTAAA+AGG 0.217002 1.3:+23137861 None:intergenic
TGTGGCAATGATTTCAAAAT+TGG 0.222370 1.3:+23138310 None:intergenic
TTTGATGGAGTTATCAATTC+AGG 0.239710 1.3:+23140944 None:intergenic
GTTCCTTTGTTGACGGGTTC+TGG 0.244415 1.3:+23138437 None:intergenic
GATGTTACTCTTAAAGAATT+TGG 0.276060 1.3:-23140665 MS.gene99515:CDS
CACTTTGAATACTAGAGATT+TGG 0.294557 1.3:+23140979 None:intergenic
GGATTTAGAGATGTTGATTT+TGG 0.298021 1.3:-23137960 MS.gene99515:CDS
TTATAACCATGATCCAAATA+TGG 0.301048 1.3:-23140840 MS.gene99515:CDS
AAATCATTGCCACATACTTT+TGG 0.305492 1.3:-23138301 MS.gene99515:CDS
GAGCTTCTTTATGATGTTCC+TGG 0.318598 1.3:-23140608 MS.gene99515:CDS
TATAATCCGGCAGTAGTTAA+AGG 0.320164 1.3:+23138008 None:intergenic
CAACTTCCAAATGGTTACTA+TGG 0.337065 1.3:-23138188 MS.gene99515:CDS
GGAGGGGATGGTGCTCTTCC+TGG 0.355512 1.3:-23137903 MS.gene99515:CDS
GTTCACCGCACTTTCTTCAT+TGG 0.359527 1.3:-23138392 MS.gene99515:CDS
CCTGCAGTTGTAACCGCAAC+GGG 0.366367 1.3:+23138149 None:intergenic
CCCGTTGCGGTTACAACTGC+AGG 0.385362 1.3:-23138149 MS.gene99515:CDS
CAAAATTAACCAGCCTACTA+AGG 0.403998 1.3:-23137757 MS.gene99515:CDS
AGCTCATCCAAGAAAGGAAA+TGG 0.411901 1.3:+23140626 None:intergenic
TCCCTGCCATATTTGGATCA+TGG 0.420285 1.3:+23140834 None:intergenic
GCGGTGAACCATGTCGTCTA+AGG 0.420378 1.3:+23138406 None:intergenic
CTTGCCAACGTAACGAATCT+TGG 0.424099 1.3:+23140879 None:intergenic
AGGTCCTAGTAGGAAACTTA+TGG 0.435900 1.3:-23140732 MS.gene99515:CDS
AGTAACCATTTGGAAGTTGT+TGG 0.444242 1.3:+23138192 None:intergenic
ACAAATGATACAGGAGAGAA+AGG 0.446166 1.3:-23137855 MS.gene99515:CDS
GTGTCTGATGTTACGCGTGC+TGG 0.446593 1.3:-23137981 MS.gene99515:CDS
AATTGGAGTATTGTTGCAAT+TGG 0.448429 1.3:+23138327 None:intergenic
GCGTTACCATAGTAACCATT+TGG 0.448893 1.3:+23138182 None:intergenic
CACTCCATTGCTACTTATCC+AGG 0.458160 1.3:-23140567 MS.gene99515:intron
CGTCCTCCTTTAACTACTGC+CGG 0.461233 1.3:-23138014 MS.gene99515:CDS
CTCCACACCGGTGAGATTGA+AGG 0.462106 1.3:+23138521 None:intergenic
ATCCATTTGCAGGTAGATTG+AGG 0.464119 1.3:-23140757 MS.gene99515:CDS
AACATGGTTCCTTTGTTGAC+GGG 0.466891 1.3:+23138431 None:intergenic
GGAGAGGTTGGTTAAAGAGT+TGG 0.469038 1.3:-23137796 MS.gene99515:CDS
TCCCTCAATCTACCTGCAAA+TGG 0.471845 1.3:+23140755 None:intergenic
AACCATGATCCAAATATGGC+AGG 0.473833 1.3:-23140836 MS.gene99515:CDS
CTGATGCATCACTCAATGTA+TGG 0.482975 1.3:+23138597 None:intergenic
CCATTGTGATCACCCTTAGT+AGG 0.484592 1.3:+23137744 None:intergenic
TCCATTTGCAGGTAGATTGA+GGG 0.488896 1.3:-23140756 MS.gene99515:CDS
GCACCATCCCCTCCTTTAGC+TGG 0.492161 1.3:+23137912 None:intergenic
CGCTTTAGCATTTCCCGTTG+CGG 0.494082 1.3:-23138162 MS.gene99515:CDS
GATTGAGGGAAGGTCCTAGT+AGG 0.496365 1.3:-23140742 MS.gene99515:CDS
TGTGATCACCCTTAGTAGGC+TGG 0.505166 1.3:+23137748 None:intergenic
TTGATGGAGTTATCAATTCA+GGG 0.506860 1.3:+23140945 None:intergenic
ATCAGACACTAAATATAATC+CGG 0.507875 1.3:+23137995 None:intergenic
AAAACTTATTGAAAATCCAT+TGG 0.514529 1.3:-23138126 MS.gene99515:CDS
TGAGTACATGTCACTCTTGG+TGG 0.516635 1.3:+23138473 None:intergenic
TATTGTTGCAATTGGTGAGT+TGG 0.518445 1.3:+23138335 None:intergenic
TGGTTATAAAATTGTATCAA+AGG 0.519033 1.3:+23140854 None:intergenic
GTTGACCAACAACTTCCAAA+TGG 0.522277 1.3:-23138197 MS.gene99515:CDS
TGATACAGGAGAGAAAGGTT+TGG 0.530630 1.3:-23137850 MS.gene99515:CDS
AACTCAAGTCATGGAGAGGT+TGG 0.531928 1.3:-23137808 MS.gene99515:CDS
TAAAGAAGCTCATCCAAGAA+AGG 0.532338 1.3:+23140620 None:intergenic
AACCTCTCCATGACTTGAGT+TGG 0.536373 1.3:+23137810 None:intergenic
CATGTTTCCCTTAGACGACA+TGG 0.537703 1.3:-23138414 MS.gene99515:CDS
CCCGCGAGATATCTCTCCTA+AGG 0.540283 1.3:+23138553 None:intergenic
TCCTGCAGTTGTAACCGCAA+CGG 0.546523 1.3:+23138148 None:intergenic
CGATGAGTACATGTCACTCT+TGG 0.550349 1.3:+23138470 None:intergenic
ACCATGATCCAAATATGGCA+GGG 0.552731 1.3:-23140835 MS.gene99515:CDS
AAACATGGTTCCTTTGTTGA+CGG 0.552807 1.3:+23138430 None:intergenic
GCAGAGAGCTTCTTAATGCA+AGG 0.554177 1.3:-23138499 MS.gene99515:CDS
CTCTAGTATTCAAAGTGAAG+AGG 0.559433 1.3:-23140973 MS.gene99515:CDS
GTTTATGGAGGACCAGCTAA+AGG 0.560640 1.3:-23137924 MS.gene99515:CDS
CGGTGAACCATGTCGTCTAA+GGG 0.561272 1.3:+23138407 None:intergenic
CATACATTGAGTGATGCATC+AGG 0.566369 1.3:-23138596 MS.gene99515:CDS
TCCACCATAAGTTTCCTACT+AGG 0.568105 1.3:+23140728 None:intergenic
AAATTCAATGCATATGCCAA+TGG 0.571571 1.3:+23138110 None:intergenic
AAACTTATGGTGGATTGTAA+TGG 0.572360 1.3:-23140719 MS.gene99515:CDS
TGCCAACTCAAGTCATGGAG+AGG 0.581695 1.3:-23137812 MS.gene99515:CDS
GAGTAAACCTTCAATCTCAC+CGG 0.590238 1.3:-23138528 MS.gene99515:CDS
CGAATTGCAGCTACCTCGGT+GGG 0.592586 1.3:+23138368 None:intergenic
GGACCAGCTAAAGGAGGGGA+TGG 0.594000 1.3:-23137915 MS.gene99515:CDS
CATGTCGTCTAAGGGAAACA+TGG 0.598974 1.3:+23138415 None:intergenic
AGAGCGAATTGCAGCTACCT+CGG 0.600847 1.3:+23138364 None:intergenic
ACGACAACGCCAAAAGTATG+TGG 0.602579 1.3:+23138292 None:intergenic
ATGGAGGACCAGCTAAAGGA+GGG 0.603039 1.3:-23137920 MS.gene99515:CDS
AAAATTAACCAGCCTACTAA+GGG 0.605486 1.3:-23137756 MS.gene99515:CDS
ATATGAAAGACAGCTTGACC+AGG 0.606019 1.3:+23137885 None:intergenic
TATACCTGGATAAGTAGCAA+TGG 0.608440 1.3:+23140563 None:intergenic
GTTGCAATTGGTGAGTTGGA+AGG 0.610618 1.3:+23138339 None:intergenic
AGATAAAACCACCACACTTG+AGG 0.612567 1.3:+23138636 None:intergenic
CAGAGAGCTTCTTAATGCAA+GGG 0.614681 1.3:-23138498 MS.gene99515:CDS
ATGACCAAGATTCGTTACGT+TGG 0.616650 1.3:-23140883 MS.gene99515:CDS
CAGGTAACACGCCTCAAGTG+TGG 0.617684 1.3:-23138647 MS.gene99515:CDS
GCCTTAGGAGAGATATCTCG+CGG 0.618905 1.3:-23138554 MS.gene99515:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.622508 1.3:-23137921 MS.gene99515:CDS
GATCCAGAACCCGTCAACAA+AGG 0.628800 1.3:-23138440 MS.gene99515:CDS
CAAAGAGTATATGCATTCAG+TGG 0.634352 1.3:-23138060 MS.gene99515:CDS
TCCTAGTAGGAAACTTATGG+TGG 0.635661 1.3:-23140729 MS.gene99515:CDS
AACCTTCAATCTCACCGGTG+TGG 0.645966 1.3:-23138523 MS.gene99515:CDS
TCATCCAAGAAAGGAAATGG+AGG 0.650133 1.3:+23140629 None:intergenic
CCTACTAAGGGTGATCACAA+TGG 0.657807 1.3:-23137744 MS.gene99515:CDS
GCGAATTGCAGCTACCTCGG+TGG 0.658016 1.3:+23138367 None:intergenic
AGAAGCTCTCTGCTCCACAC+CGG 0.659501 1.3:+23138509 None:intergenic
ATGGTGGATTGTAATGGTGA+AGG 0.660354 1.3:-23140713 MS.gene99515:CDS
TTAATCACATCTGAAGAACC+AGG 0.665982 1.3:+23140590 None:intergenic
TTTGCAGGTAGATTGAGGGA+AGG 0.669320 1.3:-23140752 MS.gene99515:CDS
AATCCGGCAGTAGTTAAAGG+AGG 0.679429 1.3:+23138011 None:intergenic
TGGGACCAATGAAGAAAGTG+CGG 0.689546 1.3:+23138387 None:intergenic
CCTTAGGAGAGATATCTCGC+GGG 0.690826 1.3:-23138553 MS.gene99515:CDS
GTAACACGCCTCAAGTGTGG+TGG 0.692143 1.3:-23138644 MS.gene99515:CDS
TGGAGGACCAGCTAAAGGAG+GGG 0.700713 1.3:-23137919 MS.gene99515:CDS
TTTCTTCATTGGTCCCACCG+AGG 0.730036 1.3:-23138381 MS.gene99515:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATAACATAGATTTA+AGG - chr1.3:23138585-23138604 MS.gene99515:CDS 10.0%
!! ATAAATAAGATTAAAATCTA+AGG + chr1.3:23138676-23138695 None:intergenic 10.0%
!! TAAATAAGATTAAAATCTAA+GGG + chr1.3:23138675-23138694 None:intergenic 10.0%
!!! TAAATTATTTTAATAAGTGA+AGG + chr1.3:23139806-23139825 None:intergenic 10.0%
!!! TATTTGAATCATTTTTTTTA+AGG + chr1.3:23139868-23139887 None:intergenic 10.0%
!!! TATTTTTAATATTACGAATA+CGG - chr1.3:23138385-23138404 MS.gene99515:CDS 10.0%
!! AAAAATGATTCAAATATATG+TGG - chr1.3:23139872-23139891 MS.gene99515:intron 15.0%
!! TATTAAAATCAATTGATATG+CGG + chr1.3:23140001-23140020 None:intergenic 15.0%
!!! AAAAAACCTTATTTTCTATA+TGG + chr1.3:23139288-23139307 None:intergenic 15.0%
!!! AATAATTTAAACTTTTTGTG+AGG - chr1.3:23139817-23139836 MS.gene99515:intron 15.0%
!!! CAATTTATTTTATCTTTTCA+AGG - chr1.3:23139542-23139561 MS.gene99515:intron 15.0%
!!! CCAACATAAAATATTTTATT+AGG - chr1.3:23139500-23139519 MS.gene99515:intron 15.0%
!!! CCTAATAAAATATTTTATGT+TGG + chr1.3:23139503-23139522 None:intergenic 15.0%
!!! TTAAACCTTTTTTTTTTTTG+AGG + chr1.3:23139311-23139330 None:intergenic 15.0%
!!! TTTTTCCTCAAAAAAAAAAA+AGG - chr1.3:23139303-23139322 MS.gene99515:intron 15.0%
!! AAAACTTATTGAAAATCCAT+TGG - chr1.3:23140579-23140598 MS.gene99515:CDS 20.0%
!! TGATGAATTCAAAAATGATA+TGG - chr1.3:23138638-23138657 MS.gene99515:CDS 20.0%
!! TGGTTATAAAATTGTATCAA+AGG + chr1.3:23137854-23137873 None:intergenic 20.0%
!! TGTTAAAAATGGTCATTAAT+TGG + chr1.3:23139900-23139919 None:intergenic 20.0%
!!! ATCAAATTTTGAGATCATTT+TGG + chr1.3:23139725-23139744 None:intergenic 20.0%
!!! ATCAAGAGTATTTAAAAGTT+TGG + chr1.3:23139681-23139700 None:intergenic 20.0%
!!! ATGAAAATTACACATTTTAC+AGG + chr1.3:23139415-23139434 None:intergenic 20.0%
!!! ATTTTAGTTTCTTGAGTTTT+CGG - chr1.3:23139757-23139776 MS.gene99515:intron 20.0%
!!! GATCTCAAAATTTGATTTTT+AGG - chr1.3:23139729-23139748 MS.gene99515:intron 20.0%
!!! TATTGAAGAAAAGTTTTGAA+GGG + chr1.3:23138856-23138875 None:intergenic 20.0%
!!! TCTAGGTTTTAATATCTTTT+TGG + chr1.3:23139624-23139643 None:intergenic 20.0%
!!! TGAAAATTACACATTTTACA+GGG + chr1.3:23139414-23139433 None:intergenic 20.0%
!!! TTATTGGCTAACATTAATTT+CGG - chr1.3:23138759-23138778 MS.gene99515:intron 20.0%
!!! TTCAATTCATTTTAACTTCT+AGG + chr1.3:23139641-23139660 None:intergenic 20.0%
! AAAATGCTAGATATCAAGAA+TGG - chr1.3:23138608-23138627 MS.gene99515:CDS 25.0%
! AAGAAATAGATAACATTAGG+AGG + chr1.3:23138948-23138967 None:intergenic 25.0%
! AGAAATAGATAACATTAGGA+GGG + chr1.3:23138947-23138966 None:intergenic 25.0%
! ATACAAAATTCTCCTCATTT+AGG + chr1.3:23138893-23138912 None:intergenic 25.0%
! ATCAGACACTAAATATAATC+CGG + chr1.3:23140713-23140732 None:intergenic 25.0%
! GATGTTACTCTTAAAGAATT+TGG - chr1.3:23138040-23138059 MS.gene99515:CDS 25.0%
! GATTTATGTTCCACTATTAT+AGG + chr1.3:23138487-23138506 None:intergenic 25.0%
! TACAAAATTCTCCTCATTTA+GGG + chr1.3:23138892-23138911 None:intergenic 25.0%
! TACATATTATCGGAGAAATT+TGG - chr1.3:23138183-23138202 MS.gene99515:CDS 25.0%
! TGGAAGAAATAGATAACATT+AGG + chr1.3:23138951-23138970 None:intergenic 25.0%
! TTATAACCATGATCCAAATA+TGG - chr1.3:23137865-23137884 MS.gene99515:CDS 25.0%
! TTTAGGCCATATAGAAAATA+AGG - chr1.3:23139279-23139298 MS.gene99515:intron 25.0%
!! AAAATGAATTGAAGCCTTAT+CGG - chr1.3:23139648-23139667 MS.gene99515:intron 25.0%
!! AATCTATGTGGTGTTAAAAA+TGG + chr1.3:23139911-23139930 None:intergenic 25.0%
!! ATTCCTTTTACAAATGATAC+AGG - chr1.3:23140841-23140860 MS.gene99515:CDS 25.0%
!! CTTTTCACACTACATATTAT+CGG - chr1.3:23138173-23138192 MS.gene99515:CDS 25.0%
!! GCTTTTCTAATAATATCAAC+AGG + chr1.3:23137902-23137921 None:intergenic 25.0%
!! TATTGGCTAACATTAATTTC+GGG - chr1.3:23138760-23138779 MS.gene99515:intron 25.0%
!!! GTATTGAAGAAAAGTTTTGA+AGG + chr1.3:23138857-23138876 None:intergenic 25.0%
!!! GTCACATGTTCAATTTTTTA+AGG + chr1.3:23138534-23138553 None:intergenic 25.0%
AAAATTAACCAGCCTACTAA+GGG - chr1.3:23140949-23140968 MS.gene99515:CDS 30.0%
AAATTCAATGCATATGCCAA+TGG + chr1.3:23140598-23140617 None:intergenic 30.0%
AACATAGCAATGTAACATCA+AGG + chr1.3:23139945-23139964 None:intergenic 30.0%
CACTATTATAGGAAAACATG+GGG + chr1.3:23138476-23138495 None:intergenic 30.0%
CACTTTGAATACTAGAGATT+TGG + chr1.3:23137729-23137748 None:intergenic 30.0%
CCACTATTATAGGAAAACAT+GGG + chr1.3:23138477-23138496 None:intergenic 30.0%
GAAAAGATCAATACTATACC+TGG + chr1.3:23138159-23138178 None:intergenic 30.0%
GAAATAGATAACATTAGGAG+GGG + chr1.3:23138946-23138965 None:intergenic 30.0%
GAGAAACAATTCGCTATTAT+AGG + chr1.3:23138509-23138528 None:intergenic 30.0%
GCTTTGTGGGAAAATATAAA+GGG - chr1.3:23138287-23138306 MS.gene99515:CDS 30.0%
GTAGAAAGTTCAAATCTATG+TGG + chr1.3:23139923-23139942 None:intergenic 30.0%
GTCAAAATTCAATGCAGATT+TGG + chr1.3:23139132-23139151 None:intergenic 30.0%
GTCCTTTCAATACTTATGTA+AGG + chr1.3:23139444-23139463 None:intergenic 30.0%
TCCACTATTATAGGAAAACA+TGG + chr1.3:23138478-23138497 None:intergenic 30.0%
TCTCCTGTATCATTTGTAAA+AGG + chr1.3:23140847-23140866 None:intergenic 30.0%
TGTGGCAATGATTTCAAAAT+TGG + chr1.3:23140398-23140417 None:intergenic 30.0%
TTGATGGAGTTATCAATTCA+GGG + chr1.3:23137763-23137782 None:intergenic 30.0%
TTTGATGGAGTTATCAATTC+AGG + chr1.3:23137764-23137783 None:intergenic 30.0%
! AAATCATTGCCACATACTTT+TGG - chr1.3:23140404-23140423 MS.gene99515:intron 30.0%
! GCAGATTTTATGGTTATCAA+GGG - chr1.3:23140667-23140686 MS.gene99515:CDS 30.0%
!! AAACTTATGGTGGATTGTAA+TGG - chr1.3:23137986-23138005 MS.gene99515:CDS 30.0%
!! AATTGGAGTATTGTTGCAAT+TGG + chr1.3:23140381-23140400 None:intergenic 30.0%
!! GTCCTTACATAAGTATTGAA+AGG - chr1.3:23139439-23139458 MS.gene99515:intron 30.0%
!! GTGTTTTATTATCCATTTGC+AGG - chr1.3:23137938-23137957 MS.gene99515:CDS 30.0%
!!! AGAGTTTTGGAAAAAAGGAA+AGG + chr1.3:23138980-23138999 None:intergenic 30.0%
!!! GGATTTAGAGATGTTGATTT+TGG - chr1.3:23140745-23140764 MS.gene99515:CDS 30.0%
!!! TAGAGATGTTGATTTTGGTT+GGG - chr1.3:23140750-23140769 MS.gene99515:CDS 30.0%
!!! TTAGAGATGTTGATTTTGGT+TGG - chr1.3:23140749-23140768 MS.gene99515:CDS 30.0%
AAACATGGTTCCTTTGTTGA+CGG + chr1.3:23140278-23140297 None:intergenic 35.0%
ACAAATGATACAGGAGAGAA+AGG - chr1.3:23140850-23140869 MS.gene99515:CDS 35.0%
ACCAATAGAAACAATTCGCT+AGG + chr1.3:23138435-23138454 None:intergenic 35.0%
ACTTCTTAGTTCCCTAAATG+AGG - chr1.3:23138878-23138897 MS.gene99515:intron 35.0%
AGTAACCATTTGGAAGTTGT+TGG + chr1.3:23140516-23140535 None:intergenic 35.0%
ATATGCGGTGATTAAACTTG+AGG + chr1.3:23139986-23140005 None:intergenic 35.0%
ATTTCGATCCAACGAAAAGA+TGG - chr1.3:23139159-23139178 MS.gene99515:intron 35.0%
CAAAATTAACCAGCCTACTA+AGG - chr1.3:23140948-23140967 MS.gene99515:CDS 35.0%
CAACTTCCAAATGGTTACTA+TGG - chr1.3:23140517-23140536 MS.gene99515:intron 35.0%
CTCTAGTATTCAAAGTGAAG+AGG - chr1.3:23137732-23137751 MS.gene99515:CDS 35.0%
TAAAGAAGCTCATCCAAGAA+AGG + chr1.3:23138088-23138107 None:intergenic 35.0%
TATAATCCGGCAGTAGTTAA+AGG + chr1.3:23140700-23140719 None:intergenic 35.0%
TATACCTGGATAAGTAGCAA+TGG + chr1.3:23138145-23138164 None:intergenic 35.0%
TATTGTTGCAATTGGTGAGT+TGG + chr1.3:23140373-23140392 None:intergenic 35.0%
TTAATCACATCTGAAGAACC+AGG + chr1.3:23138118-23138137 None:intergenic 35.0%
TTTCGATCCAACGAAAAGAT+GGG - chr1.3:23139160-23139179 MS.gene99515:intron 35.0%
TTTCGTTGGATCGAAATCAA+AGG + chr1.3:23139156-23139175 None:intergenic 35.0%
! AGTTTGGTAGTATTCCGATA+AGG + chr1.3:23139665-23139684 None:intergenic 35.0%
! CAAAGAGTATATGCATTCAG+TGG - chr1.3:23140645-23140664 MS.gene99515:CDS 35.0%
! CCCATGTTTTCCTATAATAG+TGG - chr1.3:23138474-23138493 MS.gene99515:CDS 35.0%
! GGCAGATTTTATGGTTATCA+AGG - chr1.3:23140666-23140685 MS.gene99515:CDS 35.0%
! GGCTTTGTGGGAAAATATAA+AGG - chr1.3:23138286-23138305 MS.gene99515:CDS 35.0%
!! ACTTGAGTTGGCAAAAAAAC+TGG + chr1.3:23140886-23140905 None:intergenic 35.0%
!! AGCGAATTGTTTCTATTGGT+TGG - chr1.3:23138435-23138454 MS.gene99515:CDS 35.0%
!! CTATCTCTAGGGCTTTATTT+GGG - chr1.3:23138239-23138258 MS.gene99515:CDS 35.0%
!!! AGAGATGTTGATTTTGGTTG+GGG - chr1.3:23140751-23140770 MS.gene99515:CDS 35.0%
!!! AGTTTTGTTTCATGAGGTGT+GGG + chr1.3:23137788-23137807 None:intergenic 35.0%
!!! CTTCAATTTTTGAGTGCCTT+AGG - chr1.3:23140136-23140155 MS.gene99515:intron 35.0%
!!! GAGAGTAGTTTTGTTTCATG+AGG + chr1.3:23137794-23137813 None:intergenic 35.0%
!!! TAGTTTTGTTTCATGAGGTG+TGG + chr1.3:23137789-23137808 None:intergenic 35.0%
!!! TTTTTTGCCAACTCAAGTCA+TGG - chr1.3:23140888-23140907 MS.gene99515:CDS 35.0%
AACATGGTTCCTTTGTTGAC+GGG + chr1.3:23140277-23140296 None:intergenic 40.0%
AACCATGATCCAAATATGGC+AGG - chr1.3:23137869-23137888 MS.gene99515:CDS 40.0%
AATTCATTTCTAACCCGTGC+AGG - chr1.3:23140039-23140058 MS.gene99515:intron 40.0%
ACACTTTCACGCTATCTCTA+GGG - chr1.3:23138228-23138247 MS.gene99515:CDS 40.0%
ACAGATGTTCACCTAAGCTT+TGG + chr1.3:23139031-23139050 None:intergenic 40.0%
ACATCTGTATCGAGATCACA+CGG - chr1.3:23139043-23139062 MS.gene99515:intron 40.0%
ACCATGATCCAAATATGGCA+GGG - chr1.3:23137870-23137889 MS.gene99515:CDS 40.0%
AGATAAAACCACCACACTTG+AGG + chr1.3:23140072-23140091 None:intergenic 40.0%
AGCTCATCCAAGAAAGGAAA+TGG + chr1.3:23138082-23138101 None:intergenic 40.0%
AGGTCCTAGTAGGAAACTTA+TGG - chr1.3:23137973-23137992 MS.gene99515:CDS 40.0%
ATAGATAACATTAGGAGGGG+AGG + chr1.3:23138943-23138962 None:intergenic 40.0%
ATATGAAAGACAGCTTGACC+AGG + chr1.3:23140823-23140842 None:intergenic 40.0%
ATCCATTTGCAGGTAGATTG+AGG - chr1.3:23137948-23137967 MS.gene99515:CDS 40.0%
ATGACCAAGATTCGTTACGT+TGG - chr1.3:23137822-23137841 MS.gene99515:CDS 40.0%
CAAACTTGCAACCAAAGCTT+AGG - chr1.3:23139017-23139036 MS.gene99515:intron 40.0%
CAATAAAATCTCAGCCTGAC+CGG + chr1.3:23138745-23138764 None:intergenic 40.0%
CAGAGAGCTTCTTAATGCAA+GGG - chr1.3:23140207-23140226 MS.gene99515:intron 40.0%
CATACATTGAGTGATGCATC+AGG - chr1.3:23140109-23140128 MS.gene99515:intron 40.0%
CTGATGCATCACTCAATGTA+TGG + chr1.3:23140111-23140130 None:intergenic 40.0%
GAAAAAAGGAAAGGAGCAAG+TGG + chr1.3:23138971-23138990 None:intergenic 40.0%
GAGCTTCTTTATGATGTTCC+TGG - chr1.3:23138097-23138116 MS.gene99515:CDS 40.0%
GAGTAAACCTTCAATCTCAC+CGG - chr1.3:23140177-23140196 MS.gene99515:intron 40.0%
GCGTTACCATAGTAACCATT+TGG + chr1.3:23140526-23140545 None:intergenic 40.0%
GTTGACCAACAACTTCCAAA+TGG - chr1.3:23140508-23140527 MS.gene99515:intron 40.0%
TAGATAACATTAGGAGGGGA+GGG + chr1.3:23138942-23138961 None:intergenic 40.0%
TCATCCAAGAAAGGAAATGG+AGG + chr1.3:23138079-23138098 None:intergenic 40.0%
TCATCCTCCATTTCCTTTCT+TGG - chr1.3:23138072-23138091 MS.gene99515:CDS 40.0%
TCCACCATAAGTTTCCTACT+AGG + chr1.3:23137980-23137999 None:intergenic 40.0%
TCCATTTGCAGGTAGATTGA+GGG - chr1.3:23137949-23137968 MS.gene99515:CDS 40.0%
TCCTAGTAGGAAACTTATGG+TGG - chr1.3:23137976-23137995 MS.gene99515:CDS 40.0%
TGATACAGGAGAGAAAGGTT+TGG - chr1.3:23140855-23140874 MS.gene99515:CDS 40.0%
! CAATCAACCCATCTTTTCGT+TGG + chr1.3:23139170-23139189 None:intergenic 40.0%
! CTAAGCTTTGGTTGCAAGTT+TGG + chr1.3:23139019-23139038 None:intergenic 40.0%
! CTTTATTTGGGAGTTTGGAG+GGG - chr1.3:23138251-23138270 MS.gene99515:CDS 40.0%
! GCATTCAGTGGCAGATTTTA+TGG - chr1.3:23140657-23140676 MS.gene99515:CDS 40.0%
!! ATGGTGGATTGTAATGGTGA+AGG - chr1.3:23137992-23138011 MS.gene99515:CDS 40.0%
!! GCCTAGCGAATTGTTTCTAT+TGG - chr1.3:23138431-23138450 MS.gene99515:CDS 40.0%
!! GCTATCTCTAGGGCTTTATT+TGG - chr1.3:23138238-23138257 MS.gene99515:CDS 40.0%
!! GCTTTATTTGGGAGTTTGGA+GGG - chr1.3:23138250-23138269 MS.gene99515:CDS 40.0%
!! TAGGGCTTTATTTGGGAGTT+TGG - chr1.3:23138246-23138265 MS.gene99515:CDS 40.0%
!!! AGGGGAGAGTTTTGGAAAAA+AGG + chr1.3:23138985-23139004 None:intergenic 40.0%
!!! GAGATGTTGATTTTGGTTGG+GGG - chr1.3:23140752-23140771 MS.gene99515:CDS 40.0%
AACCTCTCCATGACTTGAGT+TGG + chr1.3:23140898-23140917 None:intergenic 45.0%
AACTCAAGTCATGGAGAGGT+TGG - chr1.3:23140897-23140916 MS.gene99515:CDS 45.0%
AATCCGGCAGTAGTTAAAGG+AGG + chr1.3:23140697-23140716 None:intergenic 45.0%
AGCGTTATTCACAGCGAAGA+AGG + chr1.3:23138556-23138575 None:intergenic 45.0%
CACACTTTCACGCTATCTCT+AGG - chr1.3:23138227-23138246 MS.gene99515:CDS 45.0%
CATGTCGTCTAAGGGAAACA+TGG + chr1.3:23140293-23140312 None:intergenic 45.0%
CATGTTTCCCTTAGACGACA+TGG - chr1.3:23140291-23140310 MS.gene99515:intron 45.0%
CCATTGTGATCACCCTTAGT+AGG + chr1.3:23140964-23140983 None:intergenic 45.0%
CCTACTAAGGGTGATCACAA+TGG - chr1.3:23140961-23140980 MS.gene99515:CDS 45.0%
CGATGAGTACATGTCACTCT+TGG + chr1.3:23140238-23140257 None:intergenic 45.0%
CTTGCCAACGTAACGAATCT+TGG + chr1.3:23137829-23137848 None:intergenic 45.0%
CTTTGGTTGCAAGTTTGGAG+GGG + chr1.3:23139014-23139033 None:intergenic 45.0%
GCAGAGAGCTTCTTAATGCA+AGG - chr1.3:23140206-23140225 MS.gene99515:intron 45.0%
GCTTTGGTTGCAAGTTTGGA+GGG + chr1.3:23139015-23139034 None:intergenic 45.0%
GGATCTTTCCCTGCCATATT+TGG + chr1.3:23137881-23137900 None:intergenic 45.0%
GTTCACCGCACTTTCTTCAT+TGG - chr1.3:23140313-23140332 MS.gene99515:intron 45.0%
GTTTATGGAGGACCAGCTAA+AGG - chr1.3:23140781-23140800 MS.gene99515:CDS 45.0%
TCCCTCAATCTACCTGCAAA+TGG + chr1.3:23137953-23137972 None:intergenic 45.0%
TCCCTGCCATATTTGGATCA+TGG + chr1.3:23137874-23137893 None:intergenic 45.0%
TGAGTACATGTCACTCTTGG+TGG + chr1.3:23140235-23140254 None:intergenic 45.0%
TGGGACCAATGAAGAAAGTG+CGG + chr1.3:23140321-23140340 None:intergenic 45.0%
TTTGCAGGTAGATTGAGGGA+AGG - chr1.3:23137953-23137972 MS.gene99515:CDS 45.0%
TTTGGTTGCAAGTTTGGAGG+GGG + chr1.3:23139013-23139032 None:intergenic 45.0%
! ACGACAACGCCAAAAGTATG+TGG + chr1.3:23140416-23140435 None:intergenic 45.0%
! AGCTTTGGTTGCAAGTTTGG+AGG + chr1.3:23139016-23139035 None:intergenic 45.0%
! CACTCCATTGCTACTTATCC+AGG - chr1.3:23138138-23138157 MS.gene99515:CDS 45.0%
! CGGTCAGGCTGAGATTTTAT+TGG - chr1.3:23138743-23138762 MS.gene99515:intron 45.0%
! GGAGAGGTTGGTTAAAGAGT+TGG - chr1.3:23140909-23140928 MS.gene99515:CDS 45.0%
! GTTGCAATTGGTGAGTTGGA+AGG + chr1.3:23140369-23140388 None:intergenic 45.0%
!! GGCTTTATTTGGGAGTTTGG+AGG - chr1.3:23138249-23138268 MS.gene99515:CDS 45.0%
!! TCATGAGGTGTGGGTTTTGA+TGG + chr1.3:23137779-23137798 None:intergenic 45.0%
!!! AAATAAAATATATTTTTACA+AGG - chr1.3:23139568-23139587 MS.gene99515:intron 5.0%
!!! ATTTCATTTATATATTTTTT+TGG + chr1.3:23139108-23139127 None:intergenic 5.0%
!!! ATTTTTTTTCATTTAATTTT+AGG - chr1.3:23139262-23139281 MS.gene99515:intron 5.0%
AACCTTCAATCTCACCGGTG+TGG - chr1.3:23140182-23140201 MS.gene99515:intron 50.0%
ACCCATCACCCCATGATTTC+CGG - chr1.3:23138723-23138742 MS.gene99515:intron 50.0%
ACCGGAAATCATGGGGTGAT+GGG + chr1.3:23138727-23138746 None:intergenic 50.0%
AGAGCGAATTGCAGCTACCT+CGG + chr1.3:23140344-23140363 None:intergenic 50.0%
ATGGAGGACCAGCTAAAGGA+GGG - chr1.3:23140785-23140804 MS.gene99515:CDS 50.0%
ATTTGGGAGTTTGGAGGGGA+GGG - chr1.3:23138255-23138274 MS.gene99515:CDS 50.0%
CCTTAGGAGAGATATCTCGC+GGG - chr1.3:23140152-23140171 MS.gene99515:intron 50.0%
CGCTTTAGCATTTCCCGTTG+CGG - chr1.3:23140543-23140562 MS.gene99515:intron 50.0%
CGGTGAACCATGTCGTCTAA+GGG + chr1.3:23140301-23140320 None:intergenic 50.0%
CGTCCTCCTTTAACTACTGC+CGG - chr1.3:23140691-23140710 MS.gene99515:CDS 50.0%
GATCCAGAACCCGTCAACAA+AGG - chr1.3:23140265-23140284 MS.gene99515:intron 50.0%
GATTGAGGGAAGGTCCTAGT+AGG - chr1.3:23137963-23137982 MS.gene99515:CDS 50.0%
GCCTTAGGAGAGATATCTCG+CGG - chr1.3:23140151-23140170 MS.gene99515:intron 50.0%
GGTTGGGGGAAAGCTGTTTA+TGG - chr1.3:23140766-23140785 MS.gene99515:CDS 50.0%
TATGGAGGACCAGCTAAAGG+AGG - chr1.3:23140784-23140803 MS.gene99515:CDS 50.0%
TATTTGGGAGTTTGGAGGGG+AGG - chr1.3:23138254-23138273 MS.gene99515:CDS 50.0%
TCAGCCTGACCGGAAATCAT+GGG + chr1.3:23138735-23138754 None:intergenic 50.0%
TCCTGCAGTTGTAACCGCAA+CGG + chr1.3:23140560-23140579 None:intergenic 50.0%
TGCCAACTCAAGTCATGGAG+AGG - chr1.3:23140893-23140912 MS.gene99515:CDS 50.0%
TGGGGGAAAGCTGTTTATGG+AGG - chr1.3:23140769-23140788 MS.gene99515:CDS 50.0%
TGTGATCACCCTTAGTAGGC+TGG + chr1.3:23140960-23140979 None:intergenic 50.0%
TTGCAAGTTTGGAGGGGGAA+GGG + chr1.3:23139008-23139027 None:intergenic 50.0%
TTTCTTCATTGGTCCCACCG+AGG - chr1.3:23140324-23140343 MS.gene99515:intron 50.0%
!! GTTCCTTTGTTGACGGGTTC+TGG + chr1.3:23140271-23140290 None:intergenic 50.0%
AAGTTTGGAGGGGGAAGGGA+GGG + chr1.3:23139004-23139023 None:intergenic 55.0%
AGAAGCTCTCTGCTCCACAC+CGG + chr1.3:23140199-23140218 None:intergenic 55.0%
CAATTCGCTAGGCATTCGCG+AGG + chr1.3:23138424-23138443 None:intergenic 55.0%
CAGCCTGACCGGAAATCATG+GGG + chr1.3:23138734-23138753 None:intergenic 55.0%
CAGGTAACACGCCTCAAGTG+TGG - chr1.3:23140058-23140077 MS.gene99515:intron 55.0%
CCCGCGAGATATCTCTCCTA+AGG + chr1.3:23140155-23140174 None:intergenic 55.0%
CCTGCAGTTGTAACCGCAAC+GGG + chr1.3:23140559-23140578 None:intergenic 55.0%
CGAATTGCAGCTACCTCGGT+GGG + chr1.3:23140340-23140359 None:intergenic 55.0%
CTCAGCCTGACCGGAAATCA+TGG + chr1.3:23138736-23138755 None:intergenic 55.0%
CTCCACACCGGTGAGATTGA+AGG + chr1.3:23140187-23140206 None:intergenic 55.0%
CTTGAGGCGTGTTACCTGCA+CGG + chr1.3:23140056-23140075 None:intergenic 55.0%
GACCGGAAATCATGGGGTGA+TGG + chr1.3:23138728-23138747 None:intergenic 55.0%
GCGGTGAACCATGTCGTCTA+AGG + chr1.3:23140302-23140321 None:intergenic 55.0%
GTAACACGCCTCAAGTGTGG+TGG - chr1.3:23140061-23140080 MS.gene99515:intron 55.0%
GTGTCTGATGTTACGCGTGC+TGG - chr1.3:23140724-23140743 MS.gene99515:CDS 55.0%
GTTGCAAGTTTGGAGGGGGA+AGG + chr1.3:23139009-23139028 None:intergenic 55.0%
TCACCCCATGATTTCCGGTC+AGG - chr1.3:23138728-23138747 MS.gene99515:intron 55.0%
TGGAGGACCAGCTAAAGGAG+GGG - chr1.3:23140786-23140805 MS.gene99515:CDS 55.0%
TTGAGGCGTGTTACCTGCAC+GGG + chr1.3:23140055-23140074 None:intergenic 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr1.3:23138256-23138275 MS.gene99515:CDS 55.0%
! AGGGGAAGAGAGGGCTTTGT+GGG - chr1.3:23138274-23138293 MS.gene99515:CDS 55.0%
! TTAGGAGGGGAGGGCTTTAG+AGG + chr1.3:23138933-23138952 None:intergenic 55.0%
!! TAGGAGGGGAGGGCTTTAGA+GGG + chr1.3:23138932-23138951 None:intergenic 55.0%
AGTTTGGAGGGGGAAGGGAG+GGG + chr1.3:23139003-23139022 None:intergenic 60.0%
CAAGTTTGGAGGGGGAAGGG+AGG + chr1.3:23139005-23139024 None:intergenic 60.0%
CCCGTTGCGGTTACAACTGC+AGG - chr1.3:23140556-23140575 MS.gene99515:intron 60.0%
GAGGGGAAGAGAGGGCTTTG+TGG - chr1.3:23138273-23138292 MS.gene99515:CDS 60.0%
GCACCATCCCCTCCTTTAGC+TGG + chr1.3:23140796-23140815 None:intergenic 60.0%
GCGAATTGCAGCTACCTCGG+TGG + chr1.3:23140341-23140360 None:intergenic 60.0%
GGACCAGCTAAAGGAGGGGA+TGG - chr1.3:23140790-23140809 MS.gene99515:CDS 60.0%
TTGGAGGGGAGGGGAAGAGA+GGG - chr1.3:23138265-23138284 MS.gene99515:CDS 60.0%
TTTGGAGGGGAGGGGAAGAG+AGG - chr1.3:23138264-23138283 MS.gene99515:CDS 60.0%
! GGGAAGGGAGGGGAGAGTTT+TGG + chr1.3:23138993-23139012 None:intergenic 60.0%
GGAGGGGATGGTGCTCTTCC+TGG - chr1.3:23140802-23140821 MS.gene99515:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 23137722 23141005 23137722 ID=MS.gene99515
chr1.3 mRNA 23137722 23141005 23137722 ID=MS.gene99515.t1;Parent=MS.gene99515
chr1.3 exon 23140568 23141005 23140568 ID=MS.gene99515.t1.exon1;Parent=MS.gene99515.t1
chr1.3 CDS 23140568 23141005 23140568 ID=cds.MS.gene99515.t1;Parent=MS.gene99515.t1
chr1.3 exon 23137722 23138666 23137722 ID=MS.gene99515.t1.exon2;Parent=MS.gene99515.t1
chr1.3 CDS 23137722 23138666 23137722 ID=cds.MS.gene99515.t1;Parent=MS.gene99515.t1
Gene Sequence

>MS.gene99515

ATGGCCAAATCTCTAGTATTCAAAGTGAAGAGGCGTGACCCTGAATTGATAACTCCATCAAAACCCACACCTCATGAAACAAAACTACTCTCAGACATTGATGACCAAGATTCGTTACGTTGGCAAGTTCCTTTGATACAATTTTATAACCATGATCCAAATATGGCAGGGAAAGATCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGATTGAGGGAAGGTCCTAGTAGGAAACTTATGGTGGATTGTAATGGTGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATGTTACTCTTAAAGAATTTGGTGATGCTCTTCATCCTCCATTTCCTTTCTTGGATGAGCTTCTTTATGATGTTCCTGGTTCTTCAGATGTGATTAACACTCCATTGCTACTTATCCAGGTATAGTATTGATCTTTTCACACTACATATTATCGGAGAAATTTGGATTCTAGTCAGTCATGATTTTCACACTTTCACGCTATCTCTAGGGCTTTATTTGGGAGTTTGGAGGGGAGGGGAAGAGAGGGCTTTGTGGGAAAATATAAAGGGAAATAGAGAAATCTCTCACATTTTTTCAAAAGAATATTTTTTTAGAGAAAGAGAAACTAATGCTTATAATTCATATATTTTTAATATTACGAATACGGAAATGCTTCTTCTCCTCGCGAATGCCTAGCGAATTGTTTCTATTGGTTGGTCAAACTTCAACACTCCCCATGTTTTCCTATAATAGTGGAACATAAATCCTATAATAGCGAATTGTTTCTCTTCCTTAAAAAATTGAACATGTGACCTTCTTCGCTGTGAATAACGCTGCTCTTAAGAATATTATAACATAGATTTAAGGAAAATGCTAGATATCAAGAATGGATTTTCTTGATGAATTCAAAAATGATATGGCAGAATAATCACCCTTAGATTTTAATCTTATTTATTAAAGTTTCATAAAAAAAATACATAACACCCATCACCCCATGATTTCCGGTCAGGCTGAGATTTTATTGGCTAACATTAATTTCGGGACAATTTCTTGATAAAATCTTTCTTGATAAATAGCATTGCTTTAGATTTAATTTGAAGAATTAATATAAACCCTTCAAAACTTTTCTTCAATACAACTTCTTAGTTCCCTAAATGAGGAGAATTTTGTATTATGAAGAAAAATTAACCCTCTAAAGCCCTCCCCTCCTAATGTTATCTATTTCTTCCACTTGCTCCTTTCCTTTTTTCCAAAACTCTCCCCTCCCTTCCCCCTCCAAACTTGCAACCAAAGCTTAGGTGAACATCTGTATCGAGATCACACGGTTCATATTTCAAAATCAAAATAAATAAAAAATCAACTTTCCAAAAAAATATATAAATGAAATTCCAAATCTGCATTGAATTTTGACTCCTTTGATTTCGATCCAACGAAAAGATGGGTTGATTGCGTGATTGTATGAAAATCGTGACAAAGTCATCTAATTTTTATTTTTTATTTGATTTTAAGTCTCTAAAATATATTTTTTTTCATTTAATTTTAGGCCATATAGAAAATAAGGTTTTTTCCTCAAAAAAAAAAAAGGTTTAAATATAGTTTTGTTACTTGTAAGTATACAATTTTTTTTGTTTGTTTTAGTCATTAGAAGATTTGTTAATGTCAAAATTAGTCCCTGTAAAATGTGTAATTTTCATTTTAGTCCTTACATAAGTATTGAAAGGACTGAAAACTAAACATTTCACATTTTACATGATCAATTCCAACATAAAATATTTTATTAGGACTGAATTCTAATGTAAAGCAATTTATTTTATCTTTTCAAGGTCTAAATAAAATATATTTTTACAAGGTTGAAAAATAGAAAGTATTATATTTGTATGCCAAAAAGATATTAAAACCTAGAAGTTAAAATGAATTGAAGCCTTATCGGAATACTACCAAACTTTTAAATACTCTTGATTTGTTATCATAGAAGTTGTTGCCAAAATGATCTCAAAATTTGATTTTTAGGTCTATATTTTAGTTTCTTGAGTTTTCGGAGCTTAAATCTTTTATGATTAATCCTTCACTTATTAAAATAATTTAAACTTTTTGTGAGGAGTTGTTTATAATGTTCTAAACATTCCTTAAAAAAAATGATTCAAATATATGTGGTACCAATTAATGACCATTTTTAACACCACATAGATTTGAACTTTCTACCTTGATGTTACATTGCTATGTTTTTCAAACTAAGCAAGAACCTCAAGTTTAATCACCGCATATCAATTGATTTTAATAAATCTTTAATCATTCATTAATTCATTTCTAACCCGTGCAGGTAACACGCCTCAAGTGTGGTGGTTTTATCTTTGCTCTTCGTATGAACCATACATTGAGTGATGCATCAGGTATGCTTCAATTTTTGAGTGCCTTAGGAGAGATATCTCGCGGGATGAGTAAACCTTCAATCTCACCGGTGTGGAGCAGAGAGCTTCTTAATGCAAGGGATCCACCAAGAGTGACATGTACTCATCGTGAATACGATCCAGAACCCGTCAACAAAGGAACCATGTTTCCCTTAGACGACATGGTTCACCGCACTTTCTTCATTGGTCCCACCGAGGTAGCTGCAATTCGCTCTCTCCTTCCAACTCACCAATTGCAACAATACTCCAATTTTGAAATCATTGCCACATACTTTTGGCGTTGTCGTACAATAGCATTACAACTAGATTCTAACGAAGAAGTTCGCATAATCTGCATAGTCGATGCACGTAGCAAATCTGTTGACCAACAACTTCCAAATGGTTACTATGGTAACGCTTTAGCATTTCCCGTTGCGGTTACAACTGCAGGAAAACTTATTGAAAATCCATTGGCATATGCATTGAATTTAGTTAAGAAAGCAAAAGCTAATGTCAACAAAGAGTATATGCATTCAGTGGCAGATTTTATGGTTATCAAGGGTCGTCCTCCTTTAACTACTGCCGGATTATATTTAGTGTCTGATGTTACGCGTGCTGGATTTAGAGATGTTGATTTTGGTTGGGGGAAAGCTGTTTATGGAGGACCAGCTAAAGGAGGGGATGGTGCTCTTCCTGGTCAAGCTGTCTTTCATATTCCTTTTACAAATGATACAGGAGAGAAAGGTTTGGTTATACCAGTTTTTTTGCCAACTCAAGTCATGGAGAGGTTGGTTAAAGAGTTGGATAGTGTTCTTAAGAACAAAATTAACCAGCCTACTAAGGGTGATCACAATGGCATCATTAAATCTTCGTTGTAG
Protein sequence

>MS.gene99515.t1

MAKSLVFKVKRRDPELITPSKPTPHETKLLSDIDDQDSLRWQVPLIQFYNHDPNMAGKDPVDIIRKALAKTLVFYYPFAGRLREGPSRKLMVDCNGEGVLFIEADADVTLKEFGDALHPPFPFLDELLYDVPGSSDVINTPLLLIQVTRLKCGGFIFALRMNHTLSDASGMLQFLSALGEISRGMSKPSISPVWSRELLNARDPPRVTCTHREYDPEPVNKGTMFPLDDMVHRTFFIGPTEVAAIRSLLPTHQLQQYSNFEIIATYFWRCRTIALQLDSNEEVRIICIVDARSKSVDQQLPNGYYGNALAFPVAVTTAGKLIENPLAYALNLVKKAKANVNKEYMHSVADFMVIKGRPPLTTAGLYLVSDVTRAGFRDVDFGWGKAVYGGPAKGGDGALPGQAVFHIPFTNDTGEKGLVIPVFLPTQVMERLVKELDSVLKNKINQPTKGDHNGIIKSSL