AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99516

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99516.t1 MTR_1g040175 85.033 461 65 2 1 460 1 458 0.0 814
MS.gene99516.t1 MTR_1g040320 86.985 461 23 2 1 460 1 425 0.0 809
MS.gene99516.t1 MTR_1g040215 76.356 461 108 1 1 460 1 461 0.0 741
MS.gene99516.t1 MTR_1g040195 72.000 450 101 3 1 450 1 425 0.0 664
MS.gene99516.t1 MTR_1g040315 66.000 450 109 4 1 450 1 406 0.0 587
MS.gene99516.t1 MTR_7g114560 56.376 447 188 6 5 448 6 448 0.0 521
MS.gene99516.t1 MTR_7g114550 54.725 455 199 6 2 453 3 453 0.0 520
MS.gene99516.t1 MTR_7g114520 55.507 454 183 7 5 448 7 451 0.0 514
MS.gene99516.t1 MTR_5g076700 54.585 458 200 6 5 458 6 459 7.81e-180 511
MS.gene99516.t1 MTR_7g114470 55.556 450 189 7 5 448 7 451 5.53e-177 504
MS.gene99516.t1 MTR_1g081660 57.859 439 180 5 4 440 13 448 1.94e-176 502
MS.gene99516.t1 MTR_7g114490 54.292 431 191 5 5 432 6 433 2.71e-169 486
MS.gene99516.t1 MTR_0042s0190 73.401 297 78 1 165 460 1 297 1.88e-161 458
MS.gene99516.t1 MTR_0042s0190 73.707 232 60 1 230 460 1 232 8.77e-122 355
MS.gene99516.t1 MTR_7g114510 51.125 311 134 6 147 448 8 309 5.37e-101 305
MS.gene99516.t1 MTR_0502s0010 57.202 243 96 4 100 338 1 239 1.44e-95 288
MS.gene99516.t1 MTR_3g035000 61.261 222 59 3 199 419 43 238 1.78e-86 267
MS.gene99516.t1 MTR_0042s0230 84.354 147 23 0 1 147 1 147 5.16e-86 260
MS.gene99516.t1 MTR_3g098980 32.634 429 270 10 9 432 14 428 1.47e-76 246
MS.gene99516.t1 MTR_7g114420 44.251 287 127 5 165 448 1 257 5.79e-75 236
MS.gene99516.t1 MTR_8g096530 32.184 435 275 11 1 430 1 420 6.87e-73 236
MS.gene99516.t1 MTR_7g027175 32.311 424 267 11 13 430 20 429 1.29e-72 236
MS.gene99516.t1 MTR_8g039950 79.688 128 26 0 1 128 1 128 6.99e-71 221
MS.gene99516.t1 MTR_7g114500 59.333 150 61 0 2 151 3 152 1.06e-63 203
MS.gene99516.t1 MTR_3g051790 62.329 146 55 0 2 147 6 151 2.42e-59 192
MS.gene99516.t1 MTR_3g112180 33.419 389 231 12 17 391 20 394 1.22e-55 192
MS.gene99516.t1 MTR_4g007540 30.516 426 270 12 9 420 3 416 4.78e-54 187
MS.gene99516.t1 MTR_1g053315 30.385 441 287 12 17 440 26 463 8.69e-54 187
MS.gene99516.t1 MTR_8g075610 29.742 427 254 11 16 420 1 403 1.67e-52 182
MS.gene99516.t1 MTR_2g105330 28.864 440 287 12 9 436 3 428 7.94e-51 178
MS.gene99516.t1 MTR_4g055170 28.387 465 304 12 3 446 6 462 1.48e-50 178
MS.gene99516.t1 MTR_2g064495 28.864 440 287 12 9 436 3 428 4.14e-50 176
MS.gene99516.t1 MTR_3g071320 31.172 401 248 11 9 391 5 395 2.42e-46 166
MS.gene99516.t1 MTR_7g110610 29.327 416 225 13 5 411 1 356 6.66e-44 159
MS.gene99516.t1 MTR_7g084940 28.354 395 266 9 5 391 12 397 2.00e-43 159
MS.gene99516.t1 MTR_7g086000 30.097 412 229 17 17 385 12 407 1.30e-40 151
MS.gene99516.t1 MTR_3g465990 28.850 461 285 15 9 439 10 457 1.94e-40 150
MS.gene99516.t1 MTR_7g084910 27.960 397 245 11 8 391 16 384 7.15e-40 149
MS.gene99516.t1 MTR_3g465930 27.312 465 295 14 7 439 8 461 2.64e-38 145
MS.gene99516.t1 MTR_2g015430 29.897 388 258 10 9 387 2 384 2.25e-37 142
MS.gene99516.t1 MTR_3g465980 28.633 461 286 16 9 439 10 457 2.38e-37 142
MS.gene99516.t1 MTR_7g085930 27.358 424 260 15 9 392 6 421 3.10e-37 142
MS.gene99516.t1 MTR_6g053810 25.107 466 312 11 3 439 5 462 3.24e-37 142
MS.gene99516.t1 MTR_7g056087 27.416 445 275 16 16 434 11 433 5.46e-37 141
MS.gene99516.t1 MTR_3g026950 26.961 408 258 10 17 392 10 409 5.66e-37 141
MS.gene99516.t1 MTR_7g085990 27.039 466 284 18 9 434 2 451 5.87e-37 141
MS.gene99516.t1 MTR_3g465910 29.971 347 218 9 7 337 8 345 1.44e-36 141
MS.gene99516.t1 MTR_4g088765 79.452 73 15 0 1 73 1 73 1.98e-33 122
MS.gene99516.t1 MTR_4g127650 26.329 414 268 12 8 392 9 414 4.62e-33 130
MS.gene99516.t1 MTR_3g465880 27.021 470 293 15 7 439 8 464 1.91e-32 129
MS.gene99516.t1 MTR_3g465950 29.395 347 223 10 7 337 8 348 7.94e-32 127
MS.gene99516.t1 MTR_3g049150 26.733 404 256 12 17 390 10 403 8.63e-31 124
MS.gene99516.t1 MTR_5g090830 26.980 404 227 14 61 427 63 435 4.32e-30 122
MS.gene99516.t1 MTR_4g013050 24.834 451 240 16 17 430 12 400 5.64e-30 121
MS.gene99516.t1 MTR_4g097090 25.977 435 278 14 17 420 17 438 6.24e-30 121
MS.gene99516.t1 MTR_5g090810 25.217 460 273 15 7 427 8 435 2.93e-29 120
MS.gene99516.t1 MTR_5g090880 26.000 400 237 14 61 427 64 437 8.50e-27 112
MS.gene99516.t1 MTR_3g465870 28.716 296 186 10 5 283 8 295 1.26e-25 108
MS.gene99516.t1 MTR_3g450710 25.926 432 233 17 64 446 65 458 1.54e-25 109
MS.gene99516.t1 MTR_1g094790 26.328 433 243 18 15 404 9 408 6.60e-24 104
MS.gene99516.t1 MTR_5g084870 23.529 391 260 13 15 388 9 377 1.51e-23 103
MS.gene99516.t1 MTR_3g450690 25.063 395 227 14 64 422 72 433 7.28e-23 101
MS.gene99516.t1 MTR_3g086020 24.433 397 241 15 15 390 11 369 1.87e-22 99.4
MS.gene99516.t1 MTR_4g094828 25.245 408 262 16 51 432 49 439 8.73e-22 97.8
MS.gene99516.t1 MTR_0190s0020 25.123 406 262 16 48 432 47 431 9.75e-22 97.8
MS.gene99516.t1 MTR_8g467580 24.878 410 275 15 15 405 9 404 3.25e-21 96.3
MS.gene99516.t1 MTR_4g094842 25.464 377 246 14 48 399 46 412 3.29e-21 96.3
MS.gene99516.t1 MTR_3g101960 28.338 367 226 16 61 402 65 419 9.82e-21 94.7
MS.gene99516.t1 MTR_5g084850 22.051 390 266 12 15 388 9 376 1.93e-20 94.0
MS.gene99516.t1 MTR_3g101930 26.997 363 221 14 64 397 70 417 2.66e-20 93.6
MS.gene99516.t1 MTR_3g101900 25.610 410 256 16 61 445 63 448 3.68e-20 93.2
MS.gene99516.t1 MTR_8g079710 25.496 353 227 14 59 387 54 394 4.38e-20 92.8
MS.gene99516.t1 MTR_3g101970 28.729 362 217 15 61 402 66 406 1.19e-19 91.3
MS.gene99516.t1 MTR_8g041770 25.137 366 238 14 49 395 49 397 1.29e-19 91.3
MS.gene99516.t1 MTR_7g026860 26.173 405 260 18 48 432 42 427 2.16e-19 90.5
MS.gene99516.t1 MTR_4g094835 24.570 407 267 15 46 432 49 435 2.28e-19 90.5
MS.gene99516.t1 MTR_8g079970 22.333 403 256 11 15 388 9 383 2.31e-19 90.5
MS.gene99516.t1 MTR_8g079950 22.475 396 264 12 15 388 9 383 6.69e-19 89.4
MS.gene99516.t1 MTR_4g022720 25.410 366 246 14 51 399 44 399 7.44e-19 89.0
MS.gene99516.t1 MTR_4g022800 25.564 399 252 17 23 395 25 404 1.27e-18 88.2
MS.gene99516.t1 MTR_4g094838 22.973 370 236 12 53 399 53 396 9.36e-18 85.5
MS.gene99516.t1 MTR_5g084900 21.257 334 218 10 61 388 6 300 1.39e-17 84.3
MS.gene99516.t1 MTR_7g114417 67.308 52 16 1 89 139 29 80 1.94e-17 77.4
MS.gene99516.t1 MTR_4g094832 27.324 355 222 16 94 432 85 419 2.36e-17 84.3
MS.gene99516.t1 MTR_3g102020 25.936 374 226 16 61 402 66 420 1.09e-16 82.4
MS.gene99516.t1 MTR_4g022670 24.157 356 245 13 51 387 44 393 2.09e-16 81.6
MS.gene99516.t1 MTR_3g102010 26.596 376 225 15 61 402 62 420 5.38e-16 80.5
MS.gene99516.t1 MTR_4g022825 26.161 409 242 19 57 432 48 429 1.32e-15 79.0
MS.gene99516.t1 MTR_3g051820 47.312 93 45 3 367 458 1 90 6.98e-15 70.5
MS.gene99516.t1 MTR_6g045553 23.698 384 216 15 58 385 60 422 1.03e-14 76.6
MS.gene99516.t1 MTR_4g022760 28.727 275 176 11 64 323 55 324 5.46e-14 73.9
MS.gene99516.t1 MTR_2g036780 23.846 390 239 12 72 437 74 429 1.15e-13 73.2
MS.gene99516.t1 MTR_2g007880 23.059 425 251 18 6 389 14 403 2.23e-13 72.4
MS.gene99516.t1 MTR_3g026310 24.863 366 231 17 64 395 55 410 8.91e-13 70.5
MS.gene99516.t1 MTR_3g049140 31.333 150 84 5 257 390 54 200 1.28e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99516.t1 AT5G17540 54.390 467 200 9 1 460 1 461 8.02e-175 498
MS.gene99516.t1 AT3G03480 51.802 444 199 9 5 441 15 450 5.63e-152 440
MS.gene99516.t1 AT5G63560 32.952 437 267 11 1 430 1 418 2.10e-79 253
MS.gene99516.t1 AT5G41040 33.907 407 246 11 7 404 31 423 7.09e-78 250
MS.gene99516.t1 AT5G41040 33.907 407 246 11 7 404 15 407 8.01e-78 249
MS.gene99516.t1 AT3G48720 30.575 435 284 10 1 430 1 422 8.15e-75 241
MS.gene99516.t1 AT5G48930 30.269 446 277 13 8 436 2 430 1.17e-53 186
MS.gene99516.t1 AT1G27620 29.797 443 288 10 10 446 10 435 1.15e-52 183
MS.gene99516.t1 AT3G62160 30.330 455 271 16 5 445 1 423 5.48e-50 176
MS.gene99516.t1 AT1G03390 30.964 394 247 12 14 391 27 411 1.65e-48 172
MS.gene99516.t1 AT5G57840 28.502 414 259 12 8 397 2 402 2.39e-47 169
MS.gene99516.t1 AT1G28680 28.508 449 296 14 13 444 8 448 5.30e-47 168
MS.gene99516.t1 AT2G40230 30.858 431 262 15 9 421 5 417 2.32e-45 164
MS.gene99516.t1 AT1G65450 29.759 457 275 15 7 440 16 449 7.33e-45 162
MS.gene99516.t1 AT2G19070 29.024 410 255 13 12 392 7 409 8.18e-44 160
MS.gene99516.t1 AT1G28680 29.700 367 242 11 13 368 8 369 1.56e-42 155
MS.gene99516.t1 AT3G47170 27.061 473 296 16 4 440 7 466 9.65e-39 146
MS.gene99516.t1 AT5G07080 29.280 403 248 12 9 387 14 403 4.43e-38 144
MS.gene99516.t1 AT2G23510 28.205 468 285 17 1 439 5 450 2.13e-37 142
MS.gene99516.t1 AT2G25150 27.473 455 295 16 9 441 13 454 9.90e-35 135
MS.gene99516.t1 AT1G32910 27.901 405 251 14 62 441 63 451 5.90e-33 130
MS.gene99516.t1 AT1G78990 27.390 387 234 13 62 431 65 421 7.33e-33 130
MS.gene99516.t1 AT1G31490 28.075 374 224 13 38 389 36 386 5.48e-28 115
MS.gene99516.t1 AT1G24420 26.750 400 237 15 15 388 11 380 4.11e-25 107
MS.gene99516.t1 AT1G24420 26.750 400 237 15 15 388 11 380 4.11e-25 107
MS.gene99516.t1 AT4G31910 27.342 395 241 14 25 384 24 407 5.88e-24 104
MS.gene99516.t1 AT5G23970 26.174 447 268 16 8 431 2 409 3.86e-23 101
MS.gene99516.t1 AT5G07850 27.424 361 232 13 64 402 70 422 7.67e-23 101
MS.gene99516.t1 AT3G26040 24.000 400 255 14 15 390 9 383 3.51e-22 99.0
MS.gene99516.t1 AT5G07860 26.131 398 242 12 64 437 72 441 8.67e-22 97.8
MS.gene99516.t1 AT5G16410 24.929 353 222 13 62 390 89 422 1.92e-21 97.1
MS.gene99516.t1 AT5G38130 26.551 403 245 17 23 399 48 425 2.28e-21 96.7
MS.gene99516.t1 AT1G24430 23.737 396 261 12 15 393 11 382 2.95e-20 93.2
MS.gene99516.t1 AT5G42830 27.098 417 253 15 21 402 18 418 3.77e-20 93.2
MS.gene99516.t1 AT4G15390 23.729 413 264 16 3 388 2 390 1.04e-19 91.7
MS.gene99516.t1 AT4G15390 23.729 413 264 16 3 388 2 390 1.04e-19 91.7
MS.gene99516.t1 AT3G50280 25.419 358 236 14 64 399 59 407 1.11e-19 91.7
MS.gene99516.t1 AT1G65450 27.303 304 180 11 158 440 2 285 3.17e-19 88.2
MS.gene99516.t1 AT3G50270 25.419 358 238 11 64 399 62 412 1.55e-18 88.2
MS.gene99516.t1 AT5G47980 25.751 466 277 21 15 447 11 440 1.80e-18 87.8
MS.gene99516.t1 AT5G67160 24.318 440 303 14 13 437 2 426 5.30e-18 86.3
MS.gene99516.t1 AT3G30280 22.983 409 263 16 7 388 4 387 3.76e-17 84.0
MS.gene99516.t1 AT5G23940 25.248 404 262 15 61 440 62 449 7.58e-17 83.2
MS.gene99516.t1 AT2G39980 25.436 401 246 12 53 402 51 449 2.92e-16 81.3
MS.gene99516.t1 AT3G29670 23.851 348 230 15 64 387 66 402 7.38e-13 70.9
MS.gene99516.t1 AT3G23840 23.529 289 177 9 80 353 81 340 1.06e-12 70.1
MS.gene99516.t1 AT5G47950 24.679 312 196 12 7 309 4 285 2.79e-12 68.9
MS.gene99516.t1 AT1G65450 33.333 147 93 4 7 151 16 159 2.84e-12 65.1
MS.gene99516.t1 AT3G29635 23.988 346 226 14 67 385 70 405 2.56e-11 65.9

Find 108 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTTTCCCTGCCATATT+TGG 0.126530 1.3:+23146405 None:intergenic
ATGTTACACGTGCTGGATTT+AGG 0.146152 1.3:-23143838 MS.gene99516:CDS
TGCAGCAATGATTTCAAAAT+TGG 0.167630 1.3:+23144174 None:intergenic
TTTGATGGTGTTATCAATTC+AGG 0.170516 1.3:+23146522 None:intergenic
TCTCCTTTATCATTTGTAAA+AGG 0.217002 1.3:+23143725 None:intergenic
GGATTTAGGGATGTTGATTT+TGG 0.219143 1.3:-23143824 MS.gene99516:CDS
GCATTACCATAGTAACTATT+TGG 0.237009 1.3:+23144046 None:intergenic
GATATTACTCTTAAACAATT+TGG 0.242406 1.3:-23146243 MS.gene99516:CDS
GTTCCTTTGTTGTCGGGTTC+TGG 0.275311 1.3:+23144301 None:intergenic
TCATCCTCCATTTCCTTGCT+TGG 0.281673 1.3:-23146211 MS.gene99516:CDS
CACTTTGAATACTAGAGATT+TGG 0.294557 1.3:+23146557 None:intergenic
TTATAACCATGATCCAAATA+TGG 0.301048 1.3:-23146418 MS.gene99516:CDS
TATAATCCCGCAGTAGTTAA+AGG 0.315636 1.3:+23143872 None:intergenic
ATCCATTTGCAGGTAGGTTA+AGG 0.320236 1.3:-23146335 MS.gene99516:CDS
GAACTTCTTTATGATGTTCC+TGG 0.323967 1.3:-23146186 MS.gene99516:CDS
AAATCATTGCTGCATACTTT+TGG 0.324455 1.3:-23144165 MS.gene99516:CDS
TGTTACACGTGCTGGATTTA+GGG 0.349126 1.3:-23143837 MS.gene99516:CDS
GGAGGGGATGGTGCTCTTCC+TGG 0.355512 1.3:-23143767 MS.gene99516:CDS
GTTCACCGCACTTTCTTCAT+TGG 0.359527 1.3:-23144256 MS.gene99516:CDS
CCTGCAGTTGTAACCGCAAC+GGG 0.366367 1.3:+23144013 None:intergenic
CAACTTCCAAATAGTTACTA+TGG 0.371901 1.3:-23144052 MS.gene99516:CDS
CCCGTTGCGGTTACAACTGC+AGG 0.395172 1.3:-23144013 MS.gene99516:CDS
CAAAATTAACCAGCCTACTA+AGG 0.403998 1.3:-23143621 MS.gene99516:CDS
TCCATTTGCAGGTAGGTTAA+GGG 0.408126 1.3:-23146334 MS.gene99516:CDS
ACAAATGATAAAGGAGAGAA+AGG 0.408216 1.3:-23143719 MS.gene99516:CDS
AACATGGTTCCTTTGTTGTC+GGG 0.413970 1.3:+23144295 None:intergenic
AGTTCATCCAAGCAAGGAAA+TGG 0.414422 1.3:+23146204 None:intergenic
TCCCTGCCATATTTGGATCA+TGG 0.420285 1.3:+23146412 None:intergenic
GCGGTGAACCATGTCGTCTA+AGG 0.420378 1.3:+23144270 None:intergenic
AGGTCCTAGTAGGAAACTTA+TGG 0.427564 1.3:-23146310 MS.gene99516:CDS
AGTAACTATTTGGAAGTTGT+TGG 0.428564 1.3:+23144056 None:intergenic
TCCCTTAACCTACCTGCAAA+TGG 0.437418 1.3:+23146333 None:intergenic
AATTGGAGTATTGTTGCAAT+TGG 0.448429 1.3:+23144191 None:intergenic
CGTCCTCCTTTAACTACTGC+GGG 0.454902 1.3:-23143878 MS.gene99516:CDS
CACTCCATTGCTACTTATCC+AGG 0.458160 1.3:-23146145 MS.gene99516:intron
CTCCACACCGGTGAGATTGA+AGG 0.462106 1.3:+23144385 None:intergenic
TGCTTTAGCGTTTCCCGTTG+CGG 0.463673 1.3:-23144026 MS.gene99516:CDS
GGAGAGGTTGGTTAAAGAGT+TGG 0.469038 1.3:-23143660 MS.gene99516:CDS
CTTGCCAACGTAATGAATCT+TGG 0.470742 1.3:+23146457 None:intergenic
AACCATGATCCAAATATGGC+AGG 0.473833 1.3:-23146414 MS.gene99516:CDS
AGAGAAAGGTTTGGTTATAC+CGG 0.481825 1.3:-23143705 MS.gene99516:CDS
CTGATGCATCACTCAATGTA+TGG 0.482975 1.3:+23144461 None:intergenic
AAACATGGTTCCTTTGTTGT+CGG 0.483476 1.3:+23144294 None:intergenic
TGAGTAAATGTCACTCTTGG+TGG 0.484486 1.3:+23144337 None:intergenic
CCATTGTGATCACCCTTAGT+AGG 0.484592 1.3:+23143608 None:intergenic
AAACTTATGGTTGATTGTAA+TGG 0.490791 1.3:-23146297 MS.gene99516:CDS
GCACCATCCCCTCCTTTAGC+TGG 0.492161 1.3:+23143776 None:intergenic
TTGATGGTGTTATCAATTCA+GGG 0.493333 1.3:+23146523 None:intergenic
GTGTCTGATGTTACACGTGC+TGG 0.496612 1.3:-23143845 MS.gene99516:CDS
TGTGATCACCCTTAGTAGGC+TGG 0.505166 1.3:+23143612 None:intergenic
TATTGTTGCAATTGGTGAGT+TGG 0.518445 1.3:+23144199 None:intergenic
TGGTTATAAAATTGTATCAA+AGG 0.519033 1.3:+23146432 None:intergenic
GGTTAAGGGAAGGTCCTAGT+AGG 0.521835 1.3:-23146320 MS.gene99516:CDS
AAAACTTGTTGAAAATCCAT+TGG 0.522732 1.3:-23143990 MS.gene99516:CDS
TGATAAAGGAGAGAAAGGTT+TGG 0.523809 1.3:-23143714 MS.gene99516:CDS
AACTCAAGTCATGGAGAGGT+TGG 0.531928 1.3:-23143672 MS.gene99516:CDS
AACAGATTTGCTACGTGCAT+CGG 0.534097 1.3:+23144081 None:intergenic
AACCTCTCCATGACTTGAGT+TGG 0.536373 1.3:+23143674 None:intergenic
TCAACCATAAGTTTCCTACT+AGG 0.537063 1.3:+23146306 None:intergenic
CATGTTTCCCTTAGACGACA+TGG 0.537703 1.3:-23144278 MS.gene99516:CDS
CCCGCGAGATATCTCTCCTA+AGG 0.540283 1.3:+23144417 None:intergenic
TCCTGCAGTTGTAACCGCAA+CGG 0.546523 1.3:+23144012 None:intergenic
CGATGAGTAAATGTCACTCT+TGG 0.547541 1.3:+23144334 None:intergenic
ACCATGATCCAAATATGGCA+GGG 0.552731 1.3:-23146413 MS.gene99516:CDS
GCAGAGAGCTTCTTAACGCA+AGG 0.556473 1.3:-23144363 MS.gene99516:CDS
CTCTAGTATTCAAAGTGAAG+AGG 0.559433 1.3:-23146551 MS.gene99516:CDS
GTTTATGGAGGACCAGCTAA+AGG 0.560640 1.3:-23143788 MS.gene99516:CDS
CGGTGAACCATGTCGTCTAA+GGG 0.561272 1.3:+23144271 None:intergenic
CATACATTGAGTGATGCATC+AGG 0.566369 1.3:-23144460 MS.gene99516:CDS
TTTACTATCCATTTGCAGGT+AGG 0.573968 1.3:-23146341 MS.gene99516:CDS
TGCCAACTCAAGTCATGGAG+AGG 0.581695 1.3:-23143676 MS.gene99516:CDS
ACAACTAGATTCTAACGAAG+AGG 0.582842 1.3:-23144122 MS.gene99516:CDS
TCGTCCTCCTTTAACTACTG+CGG 0.583673 1.3:-23143879 MS.gene99516:CDS
GAGTAAACCTTCAATCTCAC+CGG 0.590238 1.3:-23144392 MS.gene99516:CDS
CGAATTGCAGCTACCTCGGT+GGG 0.592586 1.3:+23144232 None:intergenic
GGACCAGCTAAAGGAGGGGA+TGG 0.594000 1.3:-23143779 MS.gene99516:CDS
AAATTCAACGCATACACCAA+TGG 0.594850 1.3:+23143974 None:intergenic
CATGTCGTCTAAGGGAAACA+TGG 0.598974 1.3:+23144279 None:intergenic
AGAGCGAATTGCAGCTACCT+CGG 0.600847 1.3:+23144228 None:intergenic
ATGGAGGACCAGCTAAAGGA+GGG 0.603039 1.3:-23143784 MS.gene99516:CDS
AAAATTAACCAGCCTACTAA+GGG 0.605486 1.3:-23143620 MS.gene99516:CDS
TATACCTGGATAAGTAGCAA+TGG 0.605830 1.3:+23146141 None:intergenic
ATATGAAAGACAGCTTGACC+AGG 0.606019 1.3:+23143749 None:intergenic
GTTGCAATTGGTGAGTTGGA+AGG 0.610618 1.3:+23144203 None:intergenic
AGATAAAACCACCACACTTG+AGG 0.612567 1.3:+23144500 None:intergenic
TAAAGAAGTTCATCCAAGCA+AGG 0.615141 1.3:+23146198 None:intergenic
TCATCCAAGCAAGGAAATGG+AGG 0.615425 1.3:+23146207 None:intergenic
GAATTTAGTTAAGAAAGCAA+AGG 0.616320 1.3:-23143957 MS.gene99516:CDS
CAGGTAACACGCCTCAAGTG+TGG 0.617684 1.3:-23144511 MS.gene99516:CDS
ATGGTTGATTGTAATGGTGA+AGG 0.618125 1.3:-23146291 MS.gene99516:CDS
GCCTTAGGAGAGATATCTCG+CGG 0.618905 1.3:-23144418 MS.gene99516:CDS
ATGACCAAGATTCATTACGT+TGG 0.619237 1.3:-23146461 MS.gene99516:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.622508 1.3:-23143785 MS.gene99516:CDS
GAACCAGAACCCGACAACAA+AGG 0.627957 1.3:-23144304 MS.gene99516:CDS
CAGAGAGCTTCTTAACGCAA+GGG 0.631889 1.3:-23144362 MS.gene99516:CDS
AACCTTCAATCTCACCGGTG+TGG 0.645966 1.3:-23144387 MS.gene99516:CDS
TTTGCAGGTAGGTTAAGGGA+AGG 0.651163 1.3:-23146330 MS.gene99516:CDS
CCTACTAAGGGTGATCACAA+TGG 0.657807 1.3:-23143608 MS.gene99516:CDS
GCGAATTGCAGCTACCTCGG+TGG 0.658016 1.3:+23144231 None:intergenic
AGAAGCTCTCTGCTCCACAC+CGG 0.659501 1.3:+23144373 None:intergenic
TTAATCACATCTGAAGAACC+AGG 0.665982 1.3:+23146168 None:intergenic
TGGGACCAATGAAGAAAGTG+CGG 0.689546 1.3:+23144251 None:intergenic
CCTTAGGAGAGATATCTCGC+GGG 0.690826 1.3:-23144417 MS.gene99516:CDS
AATCCCGCAGTAGTTAAAGG+AGG 0.692126 1.3:+23143875 None:intergenic
GTAACACGCCTCAAGTGTGG+TGG 0.692143 1.3:-23144508 MS.gene99516:CDS
TGGAGGACCAGCTAAAGGAG+GGG 0.700713 1.3:-23143783 MS.gene99516:CDS
TTTCTTCATTGGTCCCACCG+AGG 0.730036 1.3:-23144245 MS.gene99516:CDS
CAAAGAGTATATGCACTCAG+TGG 0.731748 1.3:-23143924 MS.gene99516:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTAAAATTTTGTG+GGG - chr1.3:23145081-23145100 MS.gene99516:intron 10.0%
!!! AAATATTTTTTTATAAGGAT+TGG - chr1.3:23144424-23144443 MS.gene99516:CDS 10.0%
!!! AAATTTTTTATTAATTGAAG+TGG - chr1.3:23145457-23145476 MS.gene99516:intron 10.0%
!!! TTTAAACCTTATTTTTAATA+TGG + chr1.3:23144274-23144293 None:intergenic 10.0%
!! AAAATGATTCAAATATATTG+TGG - chr1.3:23145138-23145157 MS.gene99516:intron 15.0%
!! ATAAAAAGTAGTATATTTGT+AGG - chr1.3:23144517-23144536 MS.gene99516:intron 15.0%
!!! AAGTTGAAGTAATATTTTAT+AGG - chr1.3:23145169-23145188 MS.gene99516:intron 15.0%
!!! AGTTGAAGTAATATTTTATA+GGG - chr1.3:23145170-23145189 MS.gene99516:intron 15.0%
!!! ATATTTGAATCATTTTTTGA+AGG + chr1.3:23145134-23145153 None:intergenic 15.0%
!!! CAATTTATTTTATCTTTTCA+AGG - chr1.3:23144461-23144480 MS.gene99516:CDS 15.0%
!!! TTATGTTTTATACGATTTTT+GGG + chr1.3:23144922-23144941 None:intergenic 15.0%
!!! TTCAATTCATTTTAACTTTT+AGG + chr1.3:23144560-23144579 None:intergenic 15.0%
!!! TTTATGTTTTATACGATTTT+TGG + chr1.3:23144923-23144942 None:intergenic 15.0%
!! CATGTTAAAAAATTGTTACA+AGG + chr1.3:23145360-23145379 None:intergenic 20.0%
!! GATATTACTCTTAAACAATT+TGG - chr1.3:23143904-23143923 MS.gene99516:CDS 20.0%
!! TAGAAAGTTCAAATCTTTAT+GGG + chr1.3:23145507-23145526 None:intergenic 20.0%
!! TATAACAAATGTAACATCAA+AGG + chr1.3:23145530-23145549 None:intergenic 20.0%
!! TGGTTATAAAATTGTATCAA+AGG + chr1.3:23143718-23143737 None:intergenic 20.0%
!! TTTAGGCCATATTAAAAATA+AGG - chr1.3:23144265-23144284 MS.gene99516:CDS 20.0%
!!! ATTATTTTAACAAGTGATGA+TGG + chr1.3:23145068-23145087 None:intergenic 20.0%
!!! ATTCCTTTTACAAATGATAA+AGG - chr1.3:23146419-23146438 MS.gene99516:CDS 20.0%
!!! GTTGAAGTAATATTTTATAG+GGG - chr1.3:23145171-23145190 MS.gene99516:intron 20.0%
!!! TATGTTTTATACGATTTTTG+GGG + chr1.3:23144921-23144940 None:intergenic 20.0%
!!! TTTGATTTGCTAAAAAATGA+AGG - chr1.3:23144720-23144739 MS.gene99516:intron 20.0%
! AAACTTATGGTTGATTGTAA+TGG - chr1.3:23143850-23143869 MS.gene99516:CDS 25.0%
! AATCTTTATGGGGTTAAAAA+TGG + chr1.3:23145496-23145515 None:intergenic 25.0%
! AGAAAGTTCAAATCTTTATG+GGG + chr1.3:23145506-23145525 None:intergenic 25.0%
! ATGATTCAAATATATTGTGG+TGG - chr1.3:23145141-23145160 MS.gene99516:intron 25.0%
! GAATTTAGTTAAGAAAGCAA+AGG - chr1.3:23146190-23146209 MS.gene99516:CDS 25.0%
! GTAGAAAGTTCAAATCTTTA+TGG + chr1.3:23145508-23145527 None:intergenic 25.0%
! GTCCTTTCAATACTTATTTA+AGG + chr1.3:23144361-23144380 None:intergenic 25.0%
! TATGCAGTGAATTAAACTTA+AGG + chr1.3:23145573-23145592 None:intergenic 25.0%
! TCTCCTTTATCATTTGTAAA+AGG + chr1.3:23146425-23146444 None:intergenic 25.0%
! TGATTCAAATATATTGTGGT+GGG - chr1.3:23145142-23145161 MS.gene99516:intron 25.0%
! TTATAACCATGATCCAAATA+TGG - chr1.3:23143729-23143748 MS.gene99516:CDS 25.0%
!! AAAATGAATTGAAGCCTTAT+CGG - chr1.3:23144567-23144586 MS.gene99516:intron 25.0%
!! AAAGTTTTCCGATAATATGT+AGG + chr1.3:23144048-23144067 None:intergenic 25.0%
!! AAGTTTTCCGATAATATGTA+GGG + chr1.3:23144047-23144066 None:intergenic 25.0%
!! ACATTTTTATTGAGATCACA+CGG - chr1.3:23144117-23144136 MS.gene99516:CDS 25.0%
!! ATTTTATACAACTTGTACGT+GGG + chr1.3:23144879-23144898 None:intergenic 25.0%
!! TATTTTATACAACTTGTACG+TGG + chr1.3:23144880-23144899 None:intergenic 25.0%
!!! AAAACTTGTTGAAAATCCAT+TGG - chr1.3:23146157-23146176 MS.gene99516:CDS 25.0%
!!! ATCAAGAGTATTTAAAAGCT+TGG + chr1.3:23144600-23144619 None:intergenic 25.0%
!!! ATGTTTTATACGATTTTTGG+GGG + chr1.3:23144920-23144939 None:intergenic 25.0%
!!! GATGATGGATTAATCATAAA+GGG + chr1.3:23145053-23145072 None:intergenic 25.0%
!!! GTCCTTAAATAAGTATTGAA+AGG - chr1.3:23144356-23144375 MS.gene99516:CDS 25.0%
!!! TGATGATGGATTAATCATAA+AGG + chr1.3:23145054-23145073 None:intergenic 25.0%
!!! TTATTTTAAAATTGGGCAAG+TGG - chr1.3:23145434-23145453 MS.gene99516:intron 25.0%
!!! TTGATTTGTAAAACAGTTTC+AGG - chr1.3:23144774-23144793 MS.gene99516:intron 25.0%
AAAATTAACCAGCCTACTAA+GGG - chr1.3:23146527-23146546 MS.gene99516:CDS 30.0%
AATCACAATCAAACACTTAC+GGG + chr1.3:23145262-23145281 None:intergenic 30.0%
ACAAATGATAAAGGAGAGAA+AGG - chr1.3:23146428-23146447 MS.gene99516:CDS 30.0%
AGTAACTATTTGGAAGTTGT+TGG + chr1.3:23146094-23146113 None:intergenic 30.0%
CAACTTCCAAATAGTTACTA+TGG - chr1.3:23146095-23146114 MS.gene99516:intron 30.0%
CAATCACAATCAAACACTTA+CGG + chr1.3:23145263-23145282 None:intergenic 30.0%
CACTTTGAATACTAGAGATT+TGG + chr1.3:23143593-23143612 None:intergenic 30.0%
GAAAAGATCAATTGTATACC+TGG + chr1.3:23144023-23144042 None:intergenic 30.0%
GCATTACCATAGTAACTATT+TGG + chr1.3:23146104-23146123 None:intergenic 30.0%
TGCAGCAATGATTTCAAAAT+TGG + chr1.3:23145976-23145995 None:intergenic 30.0%
TTTGCTAAAAAATGAAGGAC+AGG - chr1.3:23144725-23144744 MS.gene99516:intron 30.0%
! AAATCATTGCTGCATACTTT+TGG - chr1.3:23145982-23146001 MS.gene99516:intron 30.0%
! CTGTTTTACAAATCAAACAC+TGG + chr1.3:23144770-23144789 None:intergenic 30.0%
! CTTTTCACCCTACATATTAT+CGG - chr1.3:23144037-23144056 MS.gene99516:CDS 30.0%
! GCAGATTTTATGGTTATCAA+GGG - chr1.3:23146245-23146264 MS.gene99516:CDS 30.0%
! TTAATCATAAAGGGTGTGTT+TGG + chr1.3:23145044-23145063 None:intergenic 30.0%
!! AAGAGTATTTAAAAGCTTGG+TGG + chr1.3:23144597-23144616 None:intergenic 30.0%
!! AATTGGAGTATTGTTGCAAT+TGG + chr1.3:23145959-23145978 None:intergenic 30.0%
!! TTGATGGTGTTATCAATTCA+GGG + chr1.3:23143627-23143646 None:intergenic 30.0%
!! TTTGATGGTGTTATCAATTC+AGG + chr1.3:23143628-23143647 None:intergenic 30.0%
!!! AGCACAGGACAAATTTTTTA+CGG + chr1.3:23144957-23144976 None:intergenic 30.0%
AAATTCAACGCATACACCAA+TGG + chr1.3:23146176-23146195 None:intergenic 35.0%
ACAACTAGATTCTAACGAAG+AGG - chr1.3:23146025-23146044 MS.gene99516:intron 35.0%
ACGGGTGTGTATAAGTATAA+GGG + chr1.3:23145244-23145263 None:intergenic 35.0%
ATGACCAAGATTCATTACGT+TGG - chr1.3:23143686-23143705 MS.gene99516:CDS 35.0%
ATGGTTGATTGTAATGGTGA+AGG - chr1.3:23143856-23143875 MS.gene99516:CDS 35.0%
CAAAATTAACCAGCCTACTA+AGG - chr1.3:23146526-23146545 MS.gene99516:CDS 35.0%
CGTAAGTGTTTGATTGTGAT+TGG - chr1.3:23145261-23145280 MS.gene99516:intron 35.0%
CTCTAGTATTCAAAGTGAAG+AGG - chr1.3:23143596-23143615 MS.gene99516:CDS 35.0%
GAACTTCTTTATGATGTTCC+TGG - chr1.3:23143961-23143980 MS.gene99516:CDS 35.0%
GGAAAACTTTGCATTATAGC+CGG - chr1.3:23144058-23144077 MS.gene99516:CDS 35.0%
GTTTGCAAAACAGTAAGTAC+TGG + chr1.3:23145022-23145041 None:intergenic 35.0%
TAAAGAAGTTCATCCAAGCA+AGG + chr1.3:23143952-23143971 None:intergenic 35.0%
TACGGGTGTGTATAAGTATA+AGG + chr1.3:23145245-23145264 None:intergenic 35.0%
TATAATCCCGCAGTAGTTAA+AGG + chr1.3:23146278-23146297 None:intergenic 35.0%
TATACCTGGATAAGTAGCAA+TGG + chr1.3:23144009-23144028 None:intergenic 35.0%
TATTGTTGCAATTGGTGAGT+TGG + chr1.3:23145951-23145970 None:intergenic 35.0%
TCAACCATAAGTTTCCTACT+AGG + chr1.3:23143844-23143863 None:intergenic 35.0%
TGATAAAGGAGAGAAAGGTT+TGG - chr1.3:23146433-23146452 MS.gene99516:CDS 35.0%
TTAATCACATCTGAAGAACC+AGG + chr1.3:23143982-23144001 None:intergenic 35.0%
TTTACTATCCATTTGCAGGT+AGG - chr1.3:23143806-23143825 MS.gene99516:CDS 35.0%
! AAACATGGTTCCTTTGTTGT+CGG + chr1.3:23145856-23145875 None:intergenic 35.0%
! AGAGAAAGGTTTGGTTATAC+CGG - chr1.3:23146442-23146461 MS.gene99516:CDS 35.0%
! GAAAGCGTAAAAATCATGAC+CGG + chr1.3:23144080-23144099 None:intergenic 35.0%
! GCTTTTCGAATAACATCAAC+AGG + chr1.3:23143766-23143785 None:intergenic 35.0%
! GGCAGATTTTATGGTTATCA+AGG - chr1.3:23146244-23146263 MS.gene99516:CDS 35.0%
! GTGTTTTACTATCCATTTGC+AGG - chr1.3:23143802-23143821 MS.gene99516:CDS 35.0%
! TGTAAAACAGTTTCAGGTAC+AGG - chr1.3:23144780-23144799 MS.gene99516:intron 35.0%
! TTTCCGAGCTTAAACCTTTT+GGG - chr1.3:23144693-23144712 MS.gene99516:intron 35.0%
! TTTTCCGAGCTTAAACCTTT+TGG - chr1.3:23144692-23144711 MS.gene99516:intron 35.0%
!! ACTTGAGTTGGCAAAAAAAC+CGG + chr1.3:23146464-23146483 None:intergenic 35.0%
!!! AGTTTTGTTTCATGAGGTGT+GGG + chr1.3:23143652-23143671 None:intergenic 35.0%
!!! CTTCAATTTTTGAGTGCCTT+AGG - chr1.3:23145714-23145733 MS.gene99516:intron 35.0%
!!! GAGAGTAGTTTTGTTTCATG+AGG + chr1.3:23143658-23143677 None:intergenic 35.0%
!!! GGATTTAGGGATGTTGATTT+TGG - chr1.3:23146323-23146342 MS.gene99516:CDS 35.0%
!!! TAGGGATGTTGATTTTGGTT+GGG - chr1.3:23146328-23146347 MS.gene99516:CDS 35.0%
!!! TAGTTTTGTTTCATGAGGTG+TGG + chr1.3:23143653-23143672 None:intergenic 35.0%
!!! TTAGGGATGTTGATTTTGGT+TGG - chr1.3:23146327-23146346 MS.gene99516:CDS 35.0%
!!! TTTTTTGCCAACTCAAGTCA+TGG - chr1.3:23146466-23146485 MS.gene99516:CDS 35.0%
AACCATGATCCAAATATGGC+AGG - chr1.3:23143733-23143752 MS.gene99516:CDS 40.0%
ACACCCAAAAGGTTTAAGCT+CGG + chr1.3:23144699-23144718 None:intergenic 40.0%
ACCATGATCCAAATATGGCA+GGG - chr1.3:23143734-23143753 MS.gene99516:CDS 40.0%
AGATAAAACCACCACACTTG+AGG + chr1.3:23145650-23145669 None:intergenic 40.0%
AGGTCCTAGTAGGAAACTTA+TGG - chr1.3:23143837-23143856 MS.gene99516:CDS 40.0%
AGTTCATCCAAGCAAGGAAA+TGG + chr1.3:23143946-23143965 None:intergenic 40.0%
ATATGAAAGACAGCTTGACC+AGG + chr1.3:23146401-23146420 None:intergenic 40.0%
ATCCATTTGCAGGTAGGTTA+AGG - chr1.3:23143812-23143831 MS.gene99516:CDS 40.0%
ATGTTACACGTGCTGGATTT+AGG - chr1.3:23146309-23146328 MS.gene99516:CDS 40.0%
CAAAAAGTTGTGCTGTGGTT+TGG + chr1.3:23144855-23144874 None:intergenic 40.0%
CACAAACACATCGAAACGAA+AGG + chr1.3:23144189-23144208 None:intergenic 40.0%
CATACATTGAGTGATGCATC+AGG - chr1.3:23145687-23145706 MS.gene99516:intron 40.0%
CGATGAGTAAATGTCACTCT+TGG + chr1.3:23145816-23145835 None:intergenic 40.0%
CTGATGCATCACTCAATGTA+TGG + chr1.3:23145689-23145708 None:intergenic 40.0%
CTTGAATTGTAACTACGTGC+AGG - chr1.3:23145617-23145636 MS.gene99516:intron 40.0%
CTTGCCAACGTAATGAATCT+TGG + chr1.3:23143693-23143712 None:intergenic 40.0%
GAGTAAACCTTCAATCTCAC+CGG - chr1.3:23145755-23145774 MS.gene99516:intron 40.0%
GCAAATCAAACACACCCAAA+AGG + chr1.3:23144710-23144729 None:intergenic 40.0%
TCCATTTGCAGGTAGGTTAA+GGG - chr1.3:23143813-23143832 MS.gene99516:CDS 40.0%
TGAGTAAATGTCACTCTTGG+TGG + chr1.3:23145813-23145832 None:intergenic 40.0%
TGTTACACGTGCTGGATTTA+GGG - chr1.3:23146310-23146329 MS.gene99516:CDS 40.0%
! AACATGGTTCCTTTGTTGTC+GGG + chr1.3:23145855-23145874 None:intergenic 40.0%
! CAAAGAGTATATGCACTCAG+TGG - chr1.3:23146223-23146242 MS.gene99516:CDS 40.0%
!! AACAGATTTGCTACGTGCAT+CGG + chr1.3:23146069-23146088 None:intergenic 40.0%
!! TGGGGGACAAAATGCTATTT+TGG + chr1.3:23144903-23144922 None:intergenic 40.0%
!!! AGGGATGTTGATTTTGGTTG+GGG - chr1.3:23146329-23146348 MS.gene99516:CDS 40.0%
AACACAAGGCATAACAGCAC+AGG + chr1.3:23144972-23144991 None:intergenic 45.0%
AACCTCTCCATGACTTGAGT+TGG + chr1.3:23146476-23146495 None:intergenic 45.0%
AACTCAAGTCATGGAGAGGT+TGG - chr1.3:23146475-23146494 MS.gene99516:CDS 45.0%
AATCCCGCAGTAGTTAAAGG+AGG + chr1.3:23146275-23146294 None:intergenic 45.0%
AGACAGAACAGCACAACACA+AGG + chr1.3:23144986-23145005 None:intergenic 45.0%
CAGAGAGCTTCTTAACGCAA+GGG - chr1.3:23145785-23145804 MS.gene99516:intron 45.0%
CATGTCGTCTAAGGGAAACA+TGG + chr1.3:23145871-23145890 None:intergenic 45.0%
CATGTTTCCCTTAGACGACA+TGG - chr1.3:23145869-23145888 MS.gene99516:intron 45.0%
CCATTGTGATCACCCTTAGT+AGG + chr1.3:23146542-23146561 None:intergenic 45.0%
CCTACTAAGGGTGATCACAA+TGG - chr1.3:23146539-23146558 MS.gene99516:CDS 45.0%
GGATCTTTCCCTGCCATATT+TGG + chr1.3:23143745-23143764 None:intergenic 45.0%
GTTCACCGCACTTTCTTCAT+TGG - chr1.3:23145891-23145910 MS.gene99516:intron 45.0%
GTTTATGGAGGACCAGCTAA+AGG - chr1.3:23146359-23146378 MS.gene99516:CDS 45.0%
TACGCTTTCACGTTGTCTCT+AGG - chr1.3:23144091-23144110 MS.gene99516:CDS 45.0%
TCATCCAAGCAAGGAAATGG+AGG + chr1.3:23143943-23143962 None:intergenic 45.0%
TCATCCTCCATTTCCTTGCT+TGG - chr1.3:23143936-23143955 MS.gene99516:CDS 45.0%
TCCCTGCCATATTTGGATCA+TGG + chr1.3:23143738-23143757 None:intergenic 45.0%
TCCCTTAACCTACCTGCAAA+TGG + chr1.3:23143817-23143836 None:intergenic 45.0%
TCGTCCTCCTTTAACTACTG+CGG - chr1.3:23146268-23146287 MS.gene99516:CDS 45.0%
TGGGACAAAAAGTTGTGCTG+TGG + chr1.3:23144860-23144879 None:intergenic 45.0%
TGGGACCAATGAAGAAAGTG+CGG + chr1.3:23145899-23145918 None:intergenic 45.0%
TTTGCAGGTAGGTTAAGGGA+AGG - chr1.3:23143817-23143836 MS.gene99516:CDS 45.0%
! AGCTTGGTGGTATTCCGATA+AGG + chr1.3:23144584-23144603 None:intergenic 45.0%
! CACTCCATTGCTACTTATCC+AGG - chr1.3:23144002-23144021 MS.gene99516:CDS 45.0%
! GCACTCAGTGGCAGATTTTA+TGG - chr1.3:23146235-23146254 MS.gene99516:CDS 45.0%
! GGAGAGGTTGGTTAAAGAGT+TGG - chr1.3:23146487-23146506 MS.gene99516:CDS 45.0%
! GTTGCAATTGGTGAGTTGGA+AGG + chr1.3:23145947-23145966 None:intergenic 45.0%
! GTTTCAGGTACAGGACAAAC+TGG - chr1.3:23144789-23144808 MS.gene99516:intron 45.0%
!! TCATGAGGTGTGGGTTTTGA+TGG + chr1.3:23143643-23143662 None:intergenic 45.0%
!!! GGGATGTTGATTTTGGTTGG+GGG - chr1.3:23146330-23146349 MS.gene99516:CDS 45.0%
!!! AAAATAATTTAAAATTTTGT+GGG - chr1.3:23145080-23145099 MS.gene99516:intron 5.0%
!!! ACATAAAATATTTTTTTATA+AGG - chr1.3:23144419-23144438 MS.gene99516:CDS 5.0%
!!! ACTAAAATTTTAAATTTTAT+AGG - chr1.3:23145200-23145219 MS.gene99516:intron 5.0%
!!! ATTTTATATCATTTAATTTT+AGG - chr1.3:23144248-23144267 MS.gene99516:CDS 5.0%
!!! CAAATTTTTATTTTAAAATT+GGG - chr1.3:23145427-23145446 MS.gene99516:intron 5.0%
!!! TAAAATAATTTAAAATTTTG+TGG - chr1.3:23145079-23145098 MS.gene99516:intron 5.0%
!!! TATAAAAAAATATTTTATGT+TGG + chr1.3:23144419-23144438 None:intergenic 5.0%
!!! TCAAATTTTTATTTTAAAAT+TGG - chr1.3:23145426-23145445 MS.gene99516:intron 5.0%
!!! TTTTAAAATAAAAATTTGAT+AGG + chr1.3:23145425-23145444 None:intergenic 5.0%
AACCTTCAATCTCACCGGTG+TGG - chr1.3:23145760-23145779 MS.gene99516:intron 50.0%
ACAGGACAAACTGGAGGCAA+GGG - chr1.3:23144798-23144817 MS.gene99516:intron 50.0%
AGAGCGAATTGCAGCTACCT+CGG + chr1.3:23145922-23145941 None:intergenic 50.0%
ATGGAGGACCAGCTAAAGGA+GGG - chr1.3:23146363-23146382 MS.gene99516:CDS 50.0%
CCTTAGGAGAGATATCTCGC+GGG - chr1.3:23145730-23145749 MS.gene99516:intron 50.0%
CGGTGAACCATGTCGTCTAA+GGG + chr1.3:23145879-23145898 None:intergenic 50.0%
CGTCCTCCTTTAACTACTGC+GGG - chr1.3:23146269-23146288 MS.gene99516:CDS 50.0%
GAACCAGAACCCGACAACAA+AGG - chr1.3:23145843-23145862 MS.gene99516:intron 50.0%
GCAGAGAGCTTCTTAACGCA+AGG - chr1.3:23145784-23145803 MS.gene99516:intron 50.0%
GCCTTAGGAGAGATATCTCG+CGG - chr1.3:23145729-23145748 MS.gene99516:intron 50.0%
GGTTAAGGGAAGGTCCTAGT+AGG - chr1.3:23143827-23143846 MS.gene99516:CDS 50.0%
GGTTGGGGGAAAGCTGTTTA+TGG - chr1.3:23146344-23146363 MS.gene99516:CDS 50.0%
GTGTCTGATGTTACACGTGC+TGG - chr1.3:23146302-23146321 MS.gene99516:CDS 50.0%
TACAGGACAAACTGGAGGCA+AGG - chr1.3:23144797-23144816 MS.gene99516:intron 50.0%
TATGGAGGACCAGCTAAAGG+AGG - chr1.3:23146362-23146381 MS.gene99516:CDS 50.0%
TCAGGTACAGGACAAACTGG+AGG - chr1.3:23144792-23144811 MS.gene99516:intron 50.0%
TCCTGCAGTTGTAACCGCAA+CGG + chr1.3:23146138-23146157 None:intergenic 50.0%
TGCCAACTCAAGTCATGGAG+AGG - chr1.3:23146471-23146490 MS.gene99516:CDS 50.0%
TGCTTTAGCGTTTCCCGTTG+CGG - chr1.3:23146121-23146140 MS.gene99516:intron 50.0%
TGGGGGAAAGCTGTTTATGG+AGG - chr1.3:23146347-23146366 MS.gene99516:CDS 50.0%
TGTGATCACCCTTAGTAGGC+TGG + chr1.3:23146538-23146557 None:intergenic 50.0%
TTTCTTCATTGGTCCCACCG+AGG - chr1.3:23145902-23145921 MS.gene99516:intron 50.0%
! GTTCCTTTGTTGTCGGGTTC+TGG + chr1.3:23145849-23145868 None:intergenic 50.0%
!! AGTTGTGCTGTGGTTTGGTG+AGG + chr1.3:23144850-23144869 None:intergenic 50.0%
!! GTTGTGCTGTGGTTTGGTGA+GGG + chr1.3:23144849-23144868 None:intergenic 50.0%
ACAAACTGGAGGCAAGGGAC+AGG - chr1.3:23144803-23144822 MS.gene99516:intron 55.0%
AGAAGCTCTCTGCTCCACAC+CGG + chr1.3:23145777-23145796 None:intergenic 55.0%
CAGGTAACACGCCTCAAGTG+TGG - chr1.3:23145636-23145655 MS.gene99516:intron 55.0%
CCCGCGAGATATCTCTCCTA+AGG + chr1.3:23145733-23145752 None:intergenic 55.0%
CCTGCAGTTGTAACCGCAAC+GGG + chr1.3:23146137-23146156 None:intergenic 55.0%
CGAATTGCAGCTACCTCGGT+GGG + chr1.3:23145918-23145937 None:intergenic 55.0%
CTCCACACCGGTGAGATTGA+AGG + chr1.3:23145765-23145784 None:intergenic 55.0%
GCGGTGAACCATGTCGTCTA+AGG + chr1.3:23145880-23145899 None:intergenic 55.0%
GTAACACGCCTCAAGTGTGG+TGG - chr1.3:23145639-23145658 MS.gene99516:intron 55.0%
TGGAGGACCAGCTAAAGGAG+GGG - chr1.3:23146364-23146383 MS.gene99516:CDS 55.0%
CCCGTTGCGGTTACAACTGC+AGG - chr1.3:23146134-23146153 MS.gene99516:intron 60.0%
GCACCATCCCCTCCTTTAGC+TGG + chr1.3:23146374-23146393 None:intergenic 60.0%
GCGAATTGCAGCTACCTCGG+TGG + chr1.3:23145919-23145938 None:intergenic 60.0%
GGACCAGCTAAAGGAGGGGA+TGG - chr1.3:23146368-23146387 MS.gene99516:CDS 60.0%
GGAGGGGATGGTGCTCTTCC+TGG - chr1.3:23146380-23146399 MS.gene99516:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 23143586 23146583 23143586 ID=MS.gene99516
chr1.3 mRNA 23143586 23146583 23143586 ID=MS.gene99516.t1;Parent=MS.gene99516
chr1.3 exon 23146146 23146583 23146146 ID=MS.gene99516.t1.exon1;Parent=MS.gene99516.t1
chr1.3 CDS 23146146 23146583 23146146 ID=cds.MS.gene99516.t1;Parent=MS.gene99516.t1
chr1.3 exon 23143586 23144530 23143586 ID=MS.gene99516.t1.exon2;Parent=MS.gene99516.t1
chr1.3 CDS 23143586 23144530 23143586 ID=cds.MS.gene99516.t1;Parent=MS.gene99516.t1
Gene Sequence

>MS.gene99516

ATGGCCAAATCTCTAGTATTCAAAGTGAAGAGGCGTGACCCTGAATTGATAACACCATCAAAACCCACACCTCATGAAACAAAACTACTCTCAGACATTGATGACCAAGATTCATTACGTTGGCAAGTTCCTTTGATACAATTTTATAACCATGATCCAAATATGGCAGGGAAAGATCCTGTTGATGTTATTCGAAAAGCATTAGCAAAAACACTTGTGTTTTACTATCCATTTGCAGGTAGGTTAAGGGAAGGTCCTAGTAGGAAACTTATGGTTGATTGTAATGGTGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATATTACTCTTAAACAATTTGGTGATGCTCTTCATCCTCCATTTCCTTGCTTGGATGAACTTCTTTATGATGTTCCTGGTTCTTCAGATGTGATTAACACTCCATTGCTACTTATCCAGGTATACAATTGATCTTTTCACCCTACATATTATCGGAAAACTTTGCATTATAGCCGGTCATGATTTTTACGCTTTCACGTTGTCTCTAGGTGAACATTTTTATTGAGATCACACGGTTCATATTTTAAAATTTTTCTTCAAAATCTGCTTTGAATTTTGACTCCTTTCGTTTCGATGTGTTTGTGTGATTCTAATTTTTTTATTTGATTTTAAGTCTCTAAAATATTTTATATCATTTAATTTTAGGCCATATTAAAAATAAGGTTTAAATATAGTTTTGTTACTTGCAAATATACAATTTTTTTTTGTTTTAATCTTTACAAAATTTGTTAGTCCTTAAATAAGTATTGAAAGGACGAAAACTAAGTATTCTACATATTACAAGATCAATTCCAACATAAAATATTTTTTTATAAGGATTGGATTATGATGAAAAGCAATTTATTTTATCTTTTCAAGGTCTAAATAAAAGATATTTTCACAAGTATGAAAAATAAAAAGTAGTATATTTGTAGGACTAAAAGATATTAAAACCTAAAAGTTAAAATGAATTGAAGCCTTATCGGAATACCACCAAGCTTTTAAATACTCTTGATGTGTTATCATAGAAGTCATTGTCAAAACGATCTCAAAATTTGATTTTTAGATCTATATTTTAGTTTCTTGAGTTTTCCGAGCTTAAACCTTTTGGGTGTGTTTGATTTGCTAAAAAATGAAGGACAGGACAGAACAACTCTAGTTGTCCAGTGTTTGATTTGTAAAACAGTTTCAGGTACAGGACAAACTGGAGGCAAGGGACAGGACAAAAACTCATATTTTTGTCCCTCACCAAACCACAGCACAACTTTTTGTCCCACGTACAAGTTGTATAAAATACCAAAATAGCATTTTGTCCCCCAAAAATCGTATAAAACATAAAATTACTCATTGCCGTAAAAAATTTGTCCTGTGCTGTTATGCCTTGTGTTGTGCTGTTCTGTCTTGTACTGTTATGTCCAGTACTTACTGTTTTGCAAACCAAACACACCCTTTATGATTAATCCATCATCACTTGTTAAAATAATTTAAAATTTTGTGGGGAGTGTTGTTTATAATGTTCTAAACATGCCTTCAAAAAATGATTCAAATATATTGTGGTGGGCAAAAAGTTGAAGTAATATTTTATAGGGGCTAAAGACTAAAATTTTAAATTTTATAGGCACTAAAAATATATTTAACCCTTATACTTATACACACCCGTAAGTGTTTGATTGTGATTGGATTTTTATTATTTTGTAATTTATAACAATTTTTATTTTTTCACTATTTTTATGCATTTATCAAATCATATTCACCTTGTAACAATTTTTTAACATGTCTTAGAAATTAACATTATGTGTATAAATAAATTATGCACTGCCTATCAAATTTTTATTTTAAAATTGGGCAAGTGGAAATTTTTTATTAATTGAAGTGGTACTATTGAATGACCATTTTTAACCCCATAAAGATTTGAACTTTCTACCTTTGATGTTACATTTGTTATATTTTTTCAACTAAGCTAGAACCTTAAGTTTAATTCACTGCATATCAATTGATCTTAATAAATCTTTACTTGAATTGTAACTACGTGCAGGTAACACGCCTCAAGTGTGGTGGTTTTATCTTTGCTCTTCGTATGAACCATACATTGAGTGATGCATCAGGTATGCTTCAATTTTTGAGTGCCTTAGGAGAGATATCTCGCGGGATGAGTAAACCTTCAATCTCACCGGTGTGGAGCAGAGAGCTTCTTAACGCAAGGGATCCACCAAGAGTGACATTTACTCATCGTGAATATGAACCAGAACCCGACAACAAAGGAACCATGTTTCCCTTAGACGACATGGTTCACCGCACTTTCTTCATTGGTCCCACCGAGGTAGCTGCAATTCGCTCTCTCCTTCCAACTCACCAATTGCAACAATACTCCAATTTTGAAATCATTGCTGCATACTTTTGGCGTTGTCGTACAATAGCATTACAACTAGATTCTAACGAAGAGGTTCGCATAATCTGCATAGCCGATGCACGTAGCAAATCTGTTGACCAACAACTTCCAAATAGTTACTATGGTAATGCTTTAGCGTTTCCCGTTGCGGTTACAACTGCAGGAAAACTTGTTGAAAATCCATTGGTGTATGCGTTGAATTTAGTTAAGAAAGCAAAGGCTAACGTCGACAAAGAGTATATGCACTCAGTGGCAGATTTTATGGTTATCAAGGGTCGTCCTCCTTTAACTACTGCGGGATTATATTTAGTGTCTGATGTTACACGTGCTGGATTTAGGGATGTTGATTTTGGTTGGGGGAAAGCTGTTTATGGAGGACCAGCTAAAGGAGGGGATGGTGCTCTTCCTGGTCAAGCTGTCTTTCATATTCCTTTTACAAATGATAAAGGAGAGAAAGGTTTGGTTATACCGGTTTTTTTGCCAACTCAAGTCATGGAGAGGTTGGTTAAAGAGTTGGATAGTGTTCTTAAGAACAAAATTAACCAGCCTACTAAGGGTGATCACAATGGCATCATTAAATCTTCGTTGTAG
Protein sequence

>MS.gene99516.t1

MAKSLVFKVKRRDPELITPSKPTPHETKLLSDIDDQDSLRWQVPLIQFYNHDPNMAGKDPVDVIRKALAKTLVFYYPFAGRLREGPSRKLMVDCNGEGVLFIEADADITLKQFGDALHPPFPCLDELLYDVPGSSDVINTPLLLIQVTRLKCGGFIFALRMNHTLSDASGMLQFLSALGEISRGMSKPSISPVWSRELLNARDPPRVTFTHREYEPEPDNKGTMFPLDDMVHRTFFIGPTEVAAIRSLLPTHQLQQYSNFEIIAAYFWRCRTIALQLDSNEEVRIICIADARSKSVDQQLPNSYYGNALAFPVAVTTAGKLVENPLVYALNLVKKAKANVDKEYMHSVADFMVIKGRPPLTTAGLYLVSDVTRAGFRDVDFGWGKAVYGGPAKGGDGALPGQAVFHIPFTNDKGEKGLVIPVFLPTQVMERLVKELDSVLKNKINQPTKGDHNGIIKSSL