AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002460.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002460.01.T01 MTR_1g050510 72.687 227 44 4 50 266 29 247 4.28e-91 271
MsG0180002460.01.T01 MTR_1g050510 73.661 224 41 4 53 266 163 378 1.08e-89 272
MsG0180002460.01.T01 MTR_1g050510 71.359 206 41 4 53 248 163 360 5.59e-76 236
MsG0180002460.01.T01 MTR_0722s0010 100.000 40 0 0 227 266 1 40 2.02e-22 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 91 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATGTGTTTGTTACATTT+TGG 0.097644 1:+38654575 MsG0180002460.01.T01:three_prime_UTR
GATGCTTAGAGGAATTTAAA+TGG 0.245077 1:+38654620 MsG0180002460.01.T01:three_prime_UTR
GTCTGGCGTTGGTTGGATTC+TGG 0.256578 1:+38652768 MsG0180002460.01.T01:CDS
TCATGAGACCAAGCCAGTTT+AGG 0.280643 1:+38652620 MsG0180002460.01.T01:CDS
CATGAGACCAAGCCAGTTTA+GGG 0.292461 1:+38652621 MsG0180002460.01.T01:CDS
GCTGACAAGGATGCCAATTC+TGG 0.292723 1:+38652470 MsG0180002460.01.T01:CDS
TCATAAGTTGTAATTTGTTT+TGG 0.295787 1:-38654726 None:intergenic
TAATCTCAATGTCGTCCTTT+TGG 0.299791 1:-38652355 None:intergenic
AAAGATTCACAAAGAAGTTC+TGG 0.313328 1:+38652287 MsG0180002460.01.T01:CDS
GGAAAGAAACAAAAGTTGTT+TGG 0.316628 1:+38652446 MsG0180002460.01.T01:CDS
GTCTGGCCACCGCTGGGATA+TGG 0.336560 1:+38654112 MsG0180002460.01.T01:intron
ATTGAAGATAAAGAATCTTC+TGG 0.336889 1:+38652425 MsG0180002460.01.T01:CDS
CTGTATGATCTGATGCTTAG+AGG 0.339892 1:+38654609 MsG0180002460.01.T01:three_prime_UTR
TTAATGCTAGAGTATATAAT+GGG 0.343183 1:+38654649 MsG0180002460.01.T01:three_prime_UTR
TTGAATTTCTCTTGACGCTC+TGG 0.348647 1:-38654146 None:intergenic
TCTAAGAGAACCACTGCTTC+TGG 0.350790 1:-38651963 None:intergenic
TTTAATGCTAGAGTATATAA+TGG 0.380367 1:+38654648 MsG0180002460.01.T01:three_prime_UTR
TGTTGCAGTCTGGCCACCGC+TGG 0.390074 1:+38654105 MsG0180002460.01.T01:intron
GGTCCTGACAGCCCCTAAAC+TGG 0.393456 1:-38652633 None:intergenic
GACTTAAGTCTGGCGTTGGT+TGG 0.428464 1:+38652761 MsG0180002460.01.T01:CDS
TTTAGAAGCAGGACAAAGAC+AGG 0.433058 1:+38652192 MsG0180002460.01.T01:intron
GATGGCCGTACAGTTGGATT+AGG 0.441733 1:+38654469 MsG0180002460.01.T01:three_prime_UTR
TTAGGTCTTTAAATATTTAG+TGG 0.442731 1:+38654487 MsG0180002460.01.T01:three_prime_UTR
TTGGGATGTAGACTTAAGTC+TGG 0.444034 1:+38652751 MsG0180002460.01.T01:CDS
AGGAGAAGCGTTCAAGAGAC+GGG 0.446673 1:+38652246 MsG0180002460.01.T01:CDS
ATGATAAGTTCCACCTATGC+AGG 0.453100 1:-38654557 None:intergenic
ATGCTTAGAGGAATTTAAAT+GGG 0.459671 1:+38654621 MsG0180002460.01.T01:three_prime_UTR
AAGCCAGTTTAGGGGCTGTC+AGG 0.460365 1:+38652630 MsG0180002460.01.T01:CDS
GTCAGAGTTCTGCTCCACTT+TGG 0.463902 1:-38654351 None:intergenic
CACTCCTCCCGGTCCTCCTA+TGG 0.467320 1:+38651998 MsG0180002460.01.T01:CDS
CTAGAGTATATAATGGGGTT+GGG 0.477860 1:+38654655 MsG0180002460.01.T01:three_prime_UTR
GCTAGAGTATATAATGGGGT+TGG 0.481596 1:+38654654 MsG0180002460.01.T01:three_prime_UTR
GAAGCAGAAGGAACTTCAGC+TGG 0.482916 1:+38654408 MsG0180002460.01.T01:CDS
GCAGATGAAAGGAAGACTTC+AGG 0.487085 1:+38652585 MsG0180002460.01.T01:CDS
GAGGTACAAGAAAACATCTA+TGG 0.493897 1:+38654679 MsG0180002460.01.T01:three_prime_UTR
CGAAGAGATGGCCGTACAGT+TGG 0.497848 1:+38654463 MsG0180002460.01.T01:three_prime_UTR
TAGAGTGGCCATAGGAGGAC+CGG 0.503254 1:-38652006 None:intergenic
GTATATAATGGGGTTGGGAG+AGG 0.503933 1:+38654660 MsG0180002460.01.T01:three_prime_UTR
TGACAGCCCCTAAACTGGCT+TGG 0.504605 1:-38652628 None:intergenic
TAATGTAGATGCTGCAAAAG+TGG 0.505531 1:+38652675 MsG0180002460.01.T01:CDS
GATGCCAATTCTGGAAAAGA+TGG 0.509839 1:+38652479 MsG0180002460.01.T01:CDS
TTAGAGGAATTTAAATGGGA+TGG 0.512592 1:+38654625 MsG0180002460.01.T01:three_prime_UTR
AGCAGTATACTGCAGGTCTT+CGG 0.516574 1:+38654440 MsG0180002460.01.T01:three_prime_UTR
GGAGAAGCGTGCAACATATG+AGG 0.523159 1:+38652214 MsG0180002460.01.T01:CDS
AAGGAGAAGCGTTCAAGAGA+CGG 0.532760 1:+38652245 MsG0180002460.01.T01:CDS
AACATATGAGGAGGTGGAGA+AGG 0.535056 1:+38652226 MsG0180002460.01.T01:CDS
GTTCCTTCTGCTTCTCAGCA+CGG 0.536161 1:-38654399 None:intergenic
CTCTAAGCATCAGATCATAC+AGG 0.536465 1:-38654608 None:intergenic
TGAGTATGTTACCCCTGCAT+AGG 0.537419 1:+38654544 MsG0180002460.01.T01:three_prime_UTR
GGTCCTCCTATGGCCACTCT+AGG 0.538129 1:+38652008 MsG0180002460.01.T01:CDS
TGTAGACTTAAGTCTGGCGT+TGG 0.540031 1:+38652757 MsG0180002460.01.T01:CDS
CAAAATCACTGCAAAGTCAA+AGG 0.542117 1:-38654523 None:intergenic
GCAGGTCTTCGGCGAAGAGA+TGG 0.543464 1:+38654451 MsG0180002460.01.T01:three_prime_UTR
TGGCAATTGTAGGGTGTGAA+AGG 0.543811 1:+38654323 MsG0180002460.01.T01:intron
ATTCAAGGAAGTATGATGCC+AGG 0.546526 1:+38651921 MsG0180002461.01.T01:CDS
ACAAAATATGCCAGAAGCAG+TGG 0.546676 1:+38651953 MsG0180002460.01.T01:CDS
AAAGTTGTTTGGAGCTGACA+AGG 0.549119 1:+38652457 MsG0180002460.01.T01:CDS
CTAGAGAAGCAGTATACTGC+AGG 0.550042 1:+38654433 MsG0180002460.01.T01:exon
TTCAGAGTCTAGGAGCCAAA+AGG 0.560099 1:+38652340 MsG0180002460.01.T01:CDS
GGTCTTTAAATATTTAGTGG+TGG 0.560978 1:+38654490 MsG0180002460.01.T01:three_prime_UTR
GTTGCAGTCTGGCCACCGCT+GGG 0.564687 1:+38654106 MsG0180002460.01.T01:intron
GCGTGCAACATATGAGGAGG+TGG 0.568606 1:+38652220 MsG0180002460.01.T01:CDS
ATTCTGACAGACACTCCTCC+CGG 0.572110 1:+38651987 MsG0180002460.01.T01:CDS
CACCGTACTATTCAGAGTCT+AGG 0.572322 1:+38652330 MsG0180002460.01.T01:CDS
ATGCCTAGAGTGGCCATAGG+AGG 0.578362 1:-38652011 None:intergenic
CTCCTAGACTCTGAATAGTA+CGG 0.585065 1:-38652332 None:intergenic
CGTACTTGTCATGCCTAGAG+TGG 0.585268 1:-38652021 None:intergenic
TTCAAGGAAGTATGATGCCA+GGG 0.588177 1:+38651922 MsG0180002461.01.T01:CDS
GTATGTTACCCCTGCATAGG+TGG 0.591627 1:+38654547 MsG0180002460.01.T01:three_prime_UTR
AGAGTGGCCATAGGAGGACC+GGG 0.594703 1:-38652005 None:intergenic
TGTGAAAGGAGAAGCCAAAG+TGG 0.595822 1:+38654337 MsG0180002460.01.T01:CDS
AGAGAAATTCAACAAACTCA+TGG 0.611540 1:+38654157 MsG0180002460.01.T01:CDS
TGATAAGTTCCACCTATGCA+GGG 0.615074 1:-38654556 None:intergenic
CATTGAGATTAGTAAGAACA+AGG 0.617690 1:+38652367 MsG0180002460.01.T01:CDS
AAAGACCTAATCCAACTGTA+CGG 0.623874 1:-38654474 None:intergenic
CCTCCGTGCTGAGAAGCAGA+AGG 0.637851 1:+38654396 MsG0180002460.01.T01:CDS
ATTGTGCTGAAGCAGATGAA+AGG 0.650635 1:+38652574 MsG0180002460.01.T01:CDS
ATGAATATAGAAAACATGAG+AGG 0.668153 1:+38652047 MsG0180002460.01.T01:CDS
GGAGAAGCGTTCAAGAGACG+GGG 0.671165 1:+38652247 MsG0180002460.01.T01:CDS
GAAGCGTGCAACATATGAGG+AGG 0.672186 1:+38652217 MsG0180002460.01.T01:CDS
TAATGCTAGAGTATATAATG+GGG 0.677686 1:+38654650 MsG0180002460.01.T01:three_prime_UTR
ATGAGACCAAGCCAGTTTAG+GGG 0.678437 1:+38652622 MsG0180002460.01.T01:CDS
GTCATGCCTAGAGTGGCCAT+AGG 0.681020 1:-38652014 None:intergenic
CCTTCTGCTTCTCAGCACGG+AGG 0.694961 1:-38654396 None:intergenic
TTAGAAGCAGGACAAAGACA+GGG 0.695493 1:+38652193 MsG0180002460.01.T01:intron
ACACTGCCATATCCCAGCGG+TGG 0.697331 1:-38654118 None:intergenic
GATAAGTTCCACCTATGCAG+GGG 0.699719 1:-38654555 None:intergenic
TATTGCCACTATCAAAACAG+TGG 0.716188 1:-38652654 None:intergenic
ACTCTGAATAGTACGGTGCA+CGG 0.717383 1:-38652325 None:intergenic
GTGGCCATAGGAGGACCGGG+AGG 0.727372 1:-38652002 None:intergenic
CAAACACTGCCATATCCCAG+CGG 0.780827 1:-38654121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATAATCTGTTATTTATT+TGG + Chr1:38652999-38653018 MsG0180002460.01.T01:intron 15.0%
!! AAAAGATGTGTATAATCTTT+GGG - Chr1:38653897-38653916 None:intergenic 20.0%
!! AGAATCAAAAGTTAAAGAAT+TGG - Chr1:38652967-38652986 None:intergenic 20.0%
!! GAAAAGAAAAAAACCATTTA+TGG - Chr1:38652929-38652948 None:intergenic 20.0%
!! GAAGTTATATATGTTGTAAT+AGG + Chr1:38653918-38653937 MsG0180002460.01.T01:intron 20.0%
!! TTAATGCTAGAGTATATAAT+GGG + Chr1:38654649-38654668 MsG0180002460.01.T01:three_prime_UTR 20.0%
!! TTAGGTCTTTAAATATTTAG+TGG + Chr1:38654487-38654506 MsG0180002460.01.T01:three_prime_UTR 20.0%
!! TTCATATATTTCTCCATAAA+TGG + Chr1:38652913-38652932 MsG0180002460.01.T01:intron 20.0%
!! TTTAATGCTAGAGTATATAA+TGG + Chr1:38654648-38654667 MsG0180002460.01.T01:three_prime_UTR 20.0%
!!! AACGGTGTTTATAAATTTTA+TGG + Chr1:38653815-38653834 MsG0180002460.01.T01:intron 20.0%
!!! AATTCCTAAAAAGCTTTTAT+TGG + Chr1:38652732-38652751 MsG0180002460.01.T01:CDS 20.0%
!!! ACTCTTTTTTGTATAACATT+AGG + Chr1:38653182-38653201 MsG0180002460.01.T01:intron 20.0%
!!! ATTCCTAAAAAGCTTTTATT+GGG + Chr1:38652733-38652752 MsG0180002460.01.T01:CDS 20.0%
!!! TGTATTTTTTCACTTAATCT+AGG + Chr1:38652511-38652530 MsG0180002460.01.T01:intron 20.0%
! AATTATCAAAGATACCTCTT+CGG - Chr1:38653126-38653145 None:intergenic 25.0%
! ATGAATATAGAAAACATGAG+AGG + Chr1:38652047-38652066 MsG0180002460.01.T01:CDS 25.0%
! ATGCTTAGAGGAATTTAAAT+GGG + Chr1:38654621-38654640 MsG0180002460.01.T01:three_prime_UTR 25.0%
! ATTACCTTATCTTCTATAAC+GGG + Chr1:38653609-38653628 MsG0180002460.01.T01:intron 25.0%
! ATTGAAGATAAAGAATCTTC+TGG + Chr1:38652425-38652444 MsG0180002460.01.T01:CDS 25.0%
! GAAAAGATGTGTATAATCTT+TGG - Chr1:38653898-38653917 None:intergenic 25.0%
! GATTACCTTATCTTCTATAA+CGG + Chr1:38653608-38653627 MsG0180002460.01.T01:intron 25.0%
! TAATGCTAGAGTATATAATG+GGG + Chr1:38654650-38654669 MsG0180002460.01.T01:three_prime_UTR 25.0%
! TAGTTGTATTGTAGCAAATA+TGG + Chr1:38653266-38653285 MsG0180002460.01.T01:intron 25.0%
! TTGTAATAGGATAATTAGAG+TGG + Chr1:38653931-38653950 MsG0180002460.01.T01:intron 25.0%
!! ATATGGTCTGTTCAAAATTT+TGG + Chr1:38653283-38653302 MsG0180002460.01.T01:intron 25.0%
!! TTTTAAACATTTGCAGAATG+TGG + Chr1:38652816-38652835 MsG0180002460.01.T01:intron 25.0%
!!! ATCATGTGTTTGTTACATTT+TGG + Chr1:38654575-38654594 MsG0180002460.01.T01:three_prime_UTR 25.0%
!!! TCATGTGTTTGTTACATTTT+GGG + Chr1:38654576-38654595 MsG0180002460.01.T01:three_prime_UTR 25.0%
!!! TTGTTATTGTACCATTTTTC+TGG + Chr1:38654013-38654032 MsG0180002460.01.T01:intron 25.0%
AAAAGAGTTCAGTAAGAACT+CGG - Chr1:38653170-38653189 None:intergenic 30.0%
AAAGATTCACAAAGAAGTTC+TGG + Chr1:38652287-38652306 MsG0180002460.01.T01:CDS 30.0%
AACTATTCAGTCAATGTAAC+AGG - Chr1:38653851-38653870 None:intergenic 30.0%
AATATCGCACACTTTAAATG+AGG + Chr1:38653632-38653651 MsG0180002460.01.T01:intron 30.0%
AATCTCCTCATGTTATTGTT+CGG - Chr1:38654216-38654235 None:intergenic 30.0%
ACAGTTAACAAGAACTTAGT+AGG + Chr1:38653037-38653056 MsG0180002460.01.T01:intron 30.0%
AGAGAAATTCAACAAACTCA+TGG + Chr1:38654157-38654176 MsG0180002460.01.T01:CDS 30.0%
AGGATAATTAGAGTGGTAAA+TGG + Chr1:38653938-38653957 MsG0180002460.01.T01:intron 30.0%
AGTTAACAAGAACTTAGTAG+GGG + Chr1:38653039-38653058 MsG0180002460.01.T01:intron 30.0%
ATAACAAATTCAAAGACCTG+AGG - Chr1:38654000-38654019 None:intergenic 30.0%
ATCCACGTGAAAAATATGAA+AGG + Chr1:38652163-38652182 MsG0180002460.01.T01:intron 30.0%
ATGGTAAGTTTGAATTCACT+CGG + Chr1:38654176-38654195 MsG0180002460.01.T01:intron 30.0%
CAGTTAACAAGAACTTAGTA+GGG + Chr1:38653038-38653057 MsG0180002460.01.T01:intron 30.0%
CATTGAGATTAGTAAGAACA+AGG + Chr1:38652367-38652386 MsG0180002460.01.T01:CDS 30.0%
GAGACAGTAAACAATATCAA+AGG - Chr1:38653785-38653804 None:intergenic 30.0%
GATGCTTAGAGGAATTTAAA+TGG + Chr1:38654620-38654639 MsG0180002460.01.T01:three_prime_UTR 30.0%
GCACAGACATAACAAATTAT+AGG - Chr1:38653550-38653569 None:intergenic 30.0%
GGAAAGAAACAAAAGTTGTT+TGG + Chr1:38652446-38652465 MsG0180002460.01.T01:CDS 30.0%
GGTCTTTAAATATTTAGTGG+TGG + Chr1:38654490-38654509 MsG0180002460.01.T01:three_prime_UTR 30.0%
TACCAAGAATCCAATATGAA+CGG - Chr1:38653245-38653264 None:intergenic 30.0%
TATTCCCGTTATAGAAGATA+AGG - Chr1:38653616-38653635 None:intergenic 30.0%
TTAGAGGAATTTAAATGGGA+TGG + Chr1:38654625-38654644 MsG0180002460.01.T01:three_prime_UTR 30.0%
TTAGGATAGAAAATCACTTG+TGG + Chr1:38653512-38653531 MsG0180002460.01.T01:intron 30.0%
! AAACTCTAAAAAAGTAGGAG+AGG + Chr1:38652394-38652413 MsG0180002460.01.T01:CDS 30.0%
! AATACCATCTTTTCCAGAAT+TGG - Chr1:38652486-38652505 None:intergenic 30.0%
! ATTTTCTATCCTAATGGTTG+AGG - Chr1:38653506-38653525 None:intergenic 30.0%
! CAAGTGATTTTCTATCCTAA+TGG - Chr1:38653512-38653531 None:intergenic 30.0%
! CATCCCAATAAAAGCTTTTT+AGG - Chr1:38652739-38652758 None:intergenic 30.0%
!! AAAAAAGTAGGAGAGGTATT+TGG + Chr1:38652401-38652420 MsG0180002460.01.T01:CDS 30.0%
!! AGAGAGTGCACAAATTTTAA+AGG - Chr1:38653425-38653444 None:intergenic 30.0%
!! AGTGCACAAATTTTAAAGGT+TGG - Chr1:38653421-38653440 None:intergenic 30.0%
!!! CTCCTACTTTTTTAGAGTTT+CGG - Chr1:38652394-38652413 None:intergenic 30.0%
AAAGACCTAATCCAACTGTA+CGG - Chr1:38654477-38654496 None:intergenic 35.0%
AATCTTTGGGCAAAATGAGA+CGG - Chr1:38653884-38653903 None:intergenic 35.0%
ACCAAGAATCCAATATGAAC+GGG - Chr1:38653244-38653263 None:intergenic 35.0%
ACTGTCTCGTTACAAGTAAA+CGG + Chr1:38653797-38653816 MsG0180002460.01.T01:intron 35.0%
AGTAAGAACTCGGCAAATAA+GGG - Chr1:38653160-38653179 None:intergenic 35.0%
ATGCCATAAAGCCAGAAAAA+TGG - Chr1:38654027-38654046 None:intergenic 35.0%
CAAAATCACTGCAAAGTCAA+AGG - Chr1:38654526-38654545 None:intergenic 35.0%
CAAAGATTACAAGCGTAGTA+TGG + Chr1:38652134-38652153 MsG0180002460.01.T01:intron 35.0%
GAGGTACAAGAAAACATCTA+TGG + Chr1:38654679-38654698 MsG0180002460.01.T01:three_prime_UTR 35.0%
TAATGTAGATGCTGCAAAAG+TGG + Chr1:38652675-38652694 MsG0180002460.01.T01:CDS 35.0%
TACTAACACATTTGAGTCTG+AGG + Chr1:38654285-38654304 MsG0180002460.01.T01:intron 35.0%
TATTGCCACTATCAAAACAG+TGG - Chr1:38652657-38652676 None:intergenic 35.0%
TCAGACTCAAATGTGTTAGT+AGG - Chr1:38654286-38654305 None:intergenic 35.0%
TTTGGCGACACTTGTAATTA+TGG + Chr1:38653301-38653320 MsG0180002460.01.T01:intron 35.0%
! CTAGAGTATATAATGGGGTT+GGG + Chr1:38654655-38654674 MsG0180002460.01.T01:three_prime_UTR 35.0%
! GACCGAAACTCTAAAAAAGT+AGG + Chr1:38652389-38652408 MsG0180002460.01.T01:CDS 35.0%
! GACCTTTCATATTTTTCACG+TGG - Chr1:38652168-38652187 None:intergenic 35.0%
! TAATCTCAATGTCGTCCTTT+TGG - Chr1:38652358-38652377 None:intergenic 35.0%
!! AAAGGTCTGATTTTAGAAGC+AGG + Chr1:38652181-38652200 MsG0180002460.01.T01:intron 35.0%
!! ATATTTTGTGTCTTGTTCCC+TGG - Chr1:38651942-38651961 None:intergenic 35.0%
!! CATTAGGAGTACTTTGAGTT+AGG + Chr1:38653198-38653217 MsG0180002460.01.T01:intron 35.0%
!!! ATTTTTCTGGCTTTATGGCA+TGG + Chr1:38654026-38654045 MsG0180002460.01.T01:intron 35.0%
!!! GTACCATTTTTCTGGCTTTA+TGG + Chr1:38654021-38654040 MsG0180002460.01.T01:intron 35.0%
!!! TTTTTCTGGCTTTATGGCAT+GGG + Chr1:38654027-38654046 MsG0180002460.01.T01:intron 35.0%
AAACTTACAGTCTGACCTGA+GGG + Chr1:38653724-38653743 MsG0180002460.01.T01:intron 40.0%
ACAAAATATGCCAGAAGCAG+TGG + Chr1:38651953-38651972 MsG0180002460.01.T01:CDS 40.0%
ACAAATTCAAAGACCTGAGG+TGG - Chr1:38653997-38654016 None:intergenic 40.0%
ACATGTGGAAAATGCATTGC+CGG - Chr1:38654088-38654107 None:intergenic 40.0%
ACCAAAGAAGAACTGTTGTG+GGG + Chr1:38653080-38653099 MsG0180002460.01.T01:intron 40.0%
ATCAAAGATACCTCTTCGGT+GGG - Chr1:38653122-38653141 None:intergenic 40.0%
ATGATAAGTTCCACCTATGC+AGG - Chr1:38654560-38654579 None:intergenic 40.0%
ATTGTGCTGAAGCAGATGAA+AGG + Chr1:38652574-38652593 MsG0180002460.01.T01:CDS 40.0%
CAAAACATGCAGTCATGAAG+AGG - Chr1:38653327-38653346 None:intergenic 40.0%
CAAAGAATTTGATTGCTGCC+CGG + Chr1:38654066-38654085 MsG0180002460.01.T01:intron 40.0%
CAATATGAACGGGGAGAAAA+GGG - Chr1:38653234-38653253 None:intergenic 40.0%
CAGTAAGAACTCGGCAAATA+AGG - Chr1:38653161-38653180 None:intergenic 40.0%
CCAAGAATCCAATATGAACG+GGG - Chr1:38653243-38653262 None:intergenic 40.0%
CTCAACTTTACCCTCACTTT+GGG - Chr1:38653685-38653704 None:intergenic 40.0%
CTCCTAGACTCTGAATAGTA+CGG - Chr1:38652335-38652354 None:intergenic 40.0%
CTCTAAGCATCAGATCATAC+AGG - Chr1:38654611-38654630 None:intergenic 40.0%
CTGTATGATCTGATGCTTAG+AGG + Chr1:38654609-38654628 MsG0180002460.01.T01:three_prime_UTR 40.0%
GACCAAAGAAGAACTGTTGT+GGG + Chr1:38653079-38653098 MsG0180002460.01.T01:intron 40.0%
GGATAATACCATTCAACCAG+AGG - Chr1:38653764-38653783 None:intergenic 40.0%
GTAAGAACTCGGCAAATAAG+GGG - Chr1:38653159-38653178 None:intergenic 40.0%
TATCAAAGATACCTCTTCGG+TGG - Chr1:38653123-38653142 None:intergenic 40.0%
TCAACTTTACCCTCACTTTG+GGG - Chr1:38653684-38653703 None:intergenic 40.0%
TCTGACCGAACAATAACATG+AGG + Chr1:38654208-38654227 MsG0180002460.01.T01:intron 40.0%
TCTGGTTGTTTGAAGGTTCT+TGG + Chr1:38652786-38652805 MsG0180002460.01.T01:intron 40.0%
TGATAAGTTCCACCTATGCA+GGG - Chr1:38654559-38654578 None:intergenic 40.0%
TTAGAAGCAGGACAAAGACA+GGG + Chr1:38652193-38652212 MsG0180002460.01.T01:intron 40.0%
TTGAATTTCTCTTGACGCTC+TGG - Chr1:38654149-38654168 None:intergenic 40.0%
TTGGGATGTAGACTTAAGTC+TGG + Chr1:38652751-38652770 MsG0180002460.01.T01:CDS 40.0%
TTTAGAAGCAGGACAAAGAC+AGG + Chr1:38652192-38652211 MsG0180002460.01.T01:intron 40.0%
! AAAGTTGTTTGGAGCTGACA+AGG + Chr1:38652457-38652476 MsG0180002460.01.T01:CDS 40.0%
! ATTTTCCACATGTTGCAGTC+TGG + Chr1:38654095-38654114 MsG0180002460.01.T01:intron 40.0%
! CTTCATGACTGCATGTTTTG+AGG + Chr1:38653327-38653346 MsG0180002460.01.T01:intron 40.0%
! GATGCCAATTCTGGAAAAGA+TGG + Chr1:38652479-38652498 MsG0180002460.01.T01:CDS 40.0%
! GCTAGAGTATATAATGGGGT+TGG + Chr1:38654654-38654673 MsG0180002460.01.T01:three_prime_UTR 40.0%
! GTTGGATTCTGGTTGTTTGA+AGG + Chr1:38652779-38652798 MsG0180002460.01.T01:intron 40.0%
! TGCAGAATGTGGATTGTTGT+GGG + Chr1:38652827-38652846 MsG0180002460.01.T01:intron 40.0%
! TTGCAGAATGTGGATTGTTG+TGG + Chr1:38652826-38652845 MsG0180002460.01.T01:intron 40.0%
! TTTGGAGTACTCGCTTTAGA+GGG + Chr1:38653468-38653487 MsG0180002460.01.T01:intron 40.0%
!! GTGGGTTGGATGTATATCAT+GGG + Chr1:38652845-38652864 MsG0180002460.01.T01:intron 40.0%
!! TGTGGGTTGGATGTATATCA+TGG + Chr1:38652844-38652863 MsG0180002460.01.T01:intron 40.0%
!! TTTTTCAGAACTGTAGGCCT+AGG - Chr1:38653970-38653989 None:intergenic 40.0%
!!! GCATGTTTTGAGGTGACAAA+AGG + Chr1:38653337-38653356 MsG0180002460.01.T01:intron 40.0%
!!! GGAGGTTTTTTCAGAACTGT+AGG - Chr1:38653976-38653995 None:intergenic 40.0%
AACATATGAGGAGGTGGAGA+AGG + Chr1:38652226-38652245 MsG0180002460.01.T01:CDS 45.0%
AATGAGGTAAAACCCACCCT+AGG + Chr1:38653648-38653667 MsG0180002460.01.T01:intron 45.0%
AATTCAAAGACCTGAGGTGG+AGG - Chr1:38653994-38654013 None:intergenic 45.0%
ACAGAGTGAGTCAGTCATTC+AGG - Chr1:38654250-38654269 None:intergenic 45.0%
ACTCTGAATAGTACGGTGCA+CGG - Chr1:38652328-38652347 None:intergenic 45.0%
AGCAGTATACTGCAGGTCTT+CGG + Chr1:38654440-38654459 MsG0180002460.01.T01:three_prime_UTR 45.0%
AGGGGCTAATTGCTTGACTA+CGG + Chr1:38653057-38653076 MsG0180002460.01.T01:intron 45.0%
AGTAAGACGCCTCAACCATT+AGG + Chr1:38653494-38653513 MsG0180002460.01.T01:intron 45.0%
ATGAGACCAAGCCAGTTTAG+GGG + Chr1:38652622-38652641 MsG0180002460.01.T01:CDS 45.0%
CAACTTTACCCTCACTTTGG+GGG - Chr1:38653683-38653702 None:intergenic 45.0%
CACCGTACTATTCAGAGTCT+AGG + Chr1:38652330-38652349 MsG0180002460.01.T01:CDS 45.0%
CATGAGACCAAGCCAGTTTA+GGG + Chr1:38652621-38652640 MsG0180002460.01.T01:CDS 45.0%
CATGTGGAAAATGCATTGCC+GGG - Chr1:38654087-38654106 None:intergenic 45.0%
CCAAAGTGAGGGTAAAGTTG+AGG + Chr1:38653683-38653702 MsG0180002460.01.T01:intron 45.0%
CCAATATGAACGGGGAGAAA+AGG - Chr1:38653235-38653254 None:intergenic 45.0%
CCCCGTTCATATTGGATTCT+TGG + Chr1:38653240-38653259 MsG0180002460.01.T01:intron 45.0%
CCTCAACTTTACCCTCACTT+TGG - Chr1:38653686-38653705 None:intergenic 45.0%
CTAGAGAAGCAGTATACTGC+AGG + Chr1:38654433-38654452 MsG0180002460.01.T01:exon 45.0%
CTCAGTTGTGCAAGTCATGA+AGG + Chr1:38653392-38653411 MsG0180002460.01.T01:intron 45.0%
GAAACTTACAGTCTGACCTG+AGG + Chr1:38653723-38653742 MsG0180002460.01.T01:intron 45.0%
GATAAGTTCCACCTATGCAG+GGG - Chr1:38654558-38654577 None:intergenic 45.0%
GCAGATGAAAGGAAGACTTC+AGG + Chr1:38652585-38652604 MsG0180002460.01.T01:CDS 45.0%
GGACCAAAGAAGAACTGTTG+TGG + Chr1:38653078-38653097 MsG0180002460.01.T01:intron 45.0%
GTACTCCAAAGTTGCTAGAG+CGG - Chr1:38653458-38653477 None:intergenic 45.0%
GTGGTAAATGGTGAGTTCCT+AGG + Chr1:38653950-38653969 MsG0180002460.01.T01:intron 45.0%
TACTCCAAAGTTGCTAGAGC+GGG - Chr1:38653457-38653476 None:intergenic 45.0%
TCACACCCTACAATTGCCAT+AGG - Chr1:38654322-38654341 None:intergenic 45.0%
TCATGAGACCAAGCCAGTTT+AGG + Chr1:38652620-38652639 MsG0180002460.01.T01:CDS 45.0%
TCGTGCCTATGGCAATTGTA+GGG + Chr1:38654314-38654333 MsG0180002460.01.T01:intron 45.0%
TCTAAGAGAACCACTGCTTC+TGG - Chr1:38651966-38651985 None:intergenic 45.0%
TCTGAAAAAACCTCCACCTC+AGG + Chr1:38653981-38654000 MsG0180002460.01.T01:intron 45.0%
TGAGTATGTTACCCCTGCAT+AGG + Chr1:38654544-38654563 MsG0180002460.01.T01:three_prime_UTR 45.0%
TGGCAATTGTAGGGTGTGAA+AGG + Chr1:38654323-38654342 MsG0180002460.01.T01:intron 45.0%
TGTAGACTTAAGTCTGGCGT+TGG + Chr1:38652757-38652776 MsG0180002460.01.T01:CDS 45.0%
TGTGAAAGGAGAAGCCAAAG+TGG + Chr1:38654337-38654356 MsG0180002460.01.T01:CDS 45.0%
TTCAGAGTCTAGGAGCCAAA+AGG + Chr1:38652340-38652359 MsG0180002460.01.T01:CDS 45.0%
! CCTTTTCTCCCCGTTCATAT+TGG + Chr1:38653232-38653251 MsG0180002460.01.T01:intron 45.0%
! GAATGTGGATTGTTGTGGGT+TGG + Chr1:38652831-38652850 MsG0180002460.01.T01:intron 45.0%
! GTATATAATGGGGTTGGGAG+AGG + Chr1:38654660-38654679 MsG0180002460.01.T01:three_prime_UTR 45.0%
!! AAGGAGAAGCGTTCAAGAGA+CGG + Chr1:38652245-38652264 MsG0180002460.01.T01:CDS 45.0%
!! CTTTGGAGTACTCGCTTTAG+AGG + Chr1:38653467-38653486 MsG0180002460.01.T01:intron 45.0%
!!! CAGGACCACTGTTTTGATAG+TGG + Chr1:38652649-38652668 MsG0180002460.01.T01:CDS 45.0%
AACTCCCGCTCTAGCAACTT+TGG + Chr1:38653450-38653469 MsG0180002460.01.T01:intron 50.0%
ACGCTCTACACGTGACTCAA+GGG + Chr1:38652078-38652097 MsG0180002460.01.T01:intron 50.0%
ATTCTGACAGACACTCCTCC+CGG + Chr1:38651987-38652006 MsG0180002460.01.T01:CDS 50.0%
CAAACACTGCCATATCCCAG+CGG - Chr1:38654124-38654143 None:intergenic 50.0%
CGTACTTGTCATGCCTAGAG+TGG - Chr1:38652024-38652043 None:intergenic 50.0%
CTCGTGCCTATGGCAATTGT+AGG + Chr1:38654313-38654332 MsG0180002460.01.T01:intron 50.0%
GAAGCAGAAGGAACTTCAGC+TGG + Chr1:38654408-38654427 MsG0180002460.01.T01:CDS 50.0%
GAAGCGTGCAACATATGAGG+AGG + Chr1:38652217-38652236 MsG0180002460.01.T01:CDS 50.0%
GATGGCCGTACAGTTGGATT+AGG + Chr1:38654469-38654488 MsG0180002460.01.T01:three_prime_UTR 50.0%
GCCCCACAACAGTTCTTCTT+TGG - Chr1:38653084-38653103 None:intergenic 50.0%
GCTGACAAGGATGCCAATTC+TGG + Chr1:38652470-38652489 MsG0180002460.01.T01:CDS 50.0%
GTATGTTACCCCTGCATAGG+TGG + Chr1:38654547-38654566 MsG0180002460.01.T01:three_prime_UTR 50.0%
GTCAGAGTTCTGCTCCACTT+TGG - Chr1:38654354-38654373 None:intergenic 50.0%
GTTCCTTCTGCTTCTCAGCA+CGG - Chr1:38654402-38654421 None:intergenic 50.0%
TACCATTCAACCAGAGGCCA+AGG - Chr1:38653758-38653777 None:intergenic 50.0%
TGAGGTTGTACTCGTGCCTA+TGG + Chr1:38654303-38654322 MsG0180002460.01.T01:intron 50.0%
! AAAGAGTACACCCACCGAAG+AGG + Chr1:38653109-38653128 MsG0180002460.01.T01:intron 50.0%
! GGAGAAGCGTGCAACATATG+AGG + Chr1:38652214-38652233 MsG0180002460.01.T01:CDS 50.0%
!! AGGAGAAGCGTTCAAGAGAC+GGG + Chr1:38652246-38652265 MsG0180002460.01.T01:CDS 50.0%
!! GACTTAAGTCTGGCGTTGGT+TGG + Chr1:38652761-38652780 MsG0180002460.01.T01:CDS 50.0%
AAGCCAGTTTAGGGGCTGTC+AGG + Chr1:38652630-38652649 MsG0180002460.01.T01:CDS 55.0%
AATCCCAGCCCCCAAAGTGA+GGG + Chr1:38653672-38653691 MsG0180002460.01.T01:intron 55.0%
ACCTGAGGGCAAACTTGCCT+TGG + Chr1:38653738-38653757 MsG0180002460.01.T01:intron 55.0%
ATGCCTAGAGTGGCCATAGG+AGG - Chr1:38652014-38652033 None:intergenic 55.0%
CACGCTCTACACGTGACTCA+AGG + Chr1:38652077-38652096 MsG0180002460.01.T01:intron 55.0%
CGAAGAGATGGCCGTACAGT+TGG + Chr1:38654463-38654482 MsG0180002460.01.T01:three_prime_UTR 55.0%
CGCTCTACACGTGACTCAAG+GGG + Chr1:38652079-38652098 MsG0180002460.01.T01:intron 55.0%
GCGTGCAACATATGAGGAGG+TGG + Chr1:38652220-38652239 MsG0180002460.01.T01:CDS 55.0%
GTCATGCCTAGAGTGGCCAT+AGG - Chr1:38652017-38652036 None:intergenic 55.0%
TAATCCCAGCCCCCAAAGTG+AGG + Chr1:38653671-38653690 MsG0180002460.01.T01:intron 55.0%
TAGAGTGGCCATAGGAGGAC+CGG - Chr1:38652009-38652028 None:intergenic 55.0%
TGACAGCCCCTAAACTGGCT+TGG - Chr1:38652631-38652650 None:intergenic 55.0%
TGAGTGAAGAGAGCGACAGC+AGG + Chr1:38652110-38652129 MsG0180002460.01.T01:intron 55.0%
TGCCTTGGCCTCTGGTTGAA+TGG + Chr1:38653753-38653772 MsG0180002460.01.T01:intron 55.0%
TTGGGGGCTGGGATTACCTA+GGG - Chr1:38653667-38653686 None:intergenic 55.0%
TTTGGGGGCTGGGATTACCT+AGG - Chr1:38653668-38653687 None:intergenic 55.0%
! TTACCCTCACTTTGGGGGCT+GGG - Chr1:38653678-38653697 None:intergenic 55.0%
! TTTACCCTCACTTTGGGGGC+TGG - Chr1:38653679-38653698 None:intergenic 55.0%
!! GGAGAAGCGTTCAAGAGACG+GGG + Chr1:38652247-38652266 MsG0180002460.01.T01:CDS 55.0%
!! GTCTGGCGTTGGTTGGATTC+TGG + Chr1:38652768-38652787 MsG0180002460.01.T01:CDS 55.0%
ACACTGCCATATCCCAGCGG+TGG - Chr1:38654121-38654140 None:intergenic 60.0%
AGAGTGGCCATAGGAGGACC+GGG - Chr1:38652008-38652027 None:intergenic 60.0%
CCTCCGTGCTGAGAAGCAGA+AGG + Chr1:38654396-38654415 MsG0180002460.01.T01:CDS 60.0%
CCTTCTGCTTCTCAGCACGG+AGG - Chr1:38654399-38654418 None:intergenic 60.0%
GCAGGTCTTCGGCGAAGAGA+TGG + Chr1:38654451-38654470 MsG0180002460.01.T01:three_prime_UTR 60.0%
GCCAAGGCAAGTTTGCCCTC+AGG - Chr1:38653742-38653761 None:intergenic 60.0%
GGCAAACTTGCCTTGGCCTC+TGG + Chr1:38653745-38653764 MsG0180002460.01.T01:intron 60.0%
GGGCTGGGATTACCTAGGGT+GGG - Chr1:38653663-38653682 None:intergenic 60.0%
GGTCCTCCTATGGCCACTCT+AGG + Chr1:38652008-38652027 MsG0180002460.01.T01:CDS 60.0%
GGTCCTGACAGCCCCTAAAC+TGG - Chr1:38652636-38652655 None:intergenic 60.0%
GGTGGCCAGACTGCAACATG+TGG - Chr1:38654103-38654122 None:intergenic 60.0%
CACTCCTCCCGGTCCTCCTA+TGG + Chr1:38651998-38652017 MsG0180002460.01.T01:CDS 65.0%
GGGGCTGGGATTACCTAGGG+TGG - Chr1:38653664-38653683 None:intergenic 65.0%
GTCTGGCCACCGCTGGGATA+TGG + Chr1:38654112-38654131 MsG0180002460.01.T01:intron 65.0%
GTTGCAGTCTGGCCACCGCT+GGG + Chr1:38654106-38654125 MsG0180002460.01.T01:intron 65.0%
TGTTGCAGTCTGGCCACCGC+TGG + Chr1:38654105-38654124 MsG0180002460.01.T01:intron 65.0%
GTGGCCATAGGAGGACCGGG+AGG - Chr1:38652005-38652024 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 38651936 38654747 38651936 ID=MsG0180002460.01;Name=MsG0180002460.01
Chr1 mRNA 38651936 38654747 38651936 ID=MsG0180002460.01.T01;Parent=MsG0180002460.01;Name=MsG0180002460.01.T01;_AED=0.49;_eAED=0.55;_QI=0|0.25|0.4|1|1|1|5|311|281
Chr1 exon 38654335 38654747 38654335 ID=MsG0180002460.01.T01:exon:12740;Parent=MsG0180002460.01.T01
Chr1 exon 38654113 38654178 38654113 ID=MsG0180002460.01.T01:exon:12739;Parent=MsG0180002460.01.T01
Chr1 exon 38652569 38652789 38652569 ID=MsG0180002460.01.T01:exon:12738;Parent=MsG0180002460.01.T01
Chr1 exon 38652197 38652500 38652197 ID=MsG0180002460.01.T01:exon:12737;Parent=MsG0180002460.01.T01
Chr1 exon 38651936 38652088 38651936 ID=MsG0180002460.01.T01:exon:12736;Parent=MsG0180002460.01.T01
Chr1 CDS 38651936 38652088 38651936 ID=MsG0180002460.01.T01:cds;Parent=MsG0180002460.01.T01
Chr1 CDS 38652197 38652500 38652197 ID=MsG0180002460.01.T01:cds;Parent=MsG0180002460.01.T01
Chr1 CDS 38652569 38652789 38652569 ID=MsG0180002460.01.T01:cds;Parent=MsG0180002460.01.T01
Chr1 CDS 38654113 38654178 38654113 ID=MsG0180002460.01.T01:cds;Parent=MsG0180002460.01.T01
Chr1 CDS 38654335 38654436 38654335 ID=MsG0180002460.01.T01:cds;Parent=MsG0180002460.01.T01
Chr1 three_prime_UTR 38654437 38654747 38654437 ID=MsG0180002460.01.T01:three_prime_utr;Parent=MsG0180002460.01.T01
Gene Sequence

>MsG0180002460.01.T01

ATGCCAGGGAACAAGACACAAAATATGCCAGAAGCAGTGGTTCTCTTAGACATTCTGACAGACACTCCTCCCGGTCCTCCTATGGCCACTCTAGGCATGACAAGTACGCAGATGAATATAGAAAACATGAGAGGTTATCATCACGCTCTACACAAGCAGGACAAAGACAGGGAGAAGCGTGCAACATATGAGGAGGTGGAGAAGGAGAAGCGTTCAAGAGACGGGGATGCTCGAGATGAAAAAAAAGATTCACAAAGAAGTTCTGGAGAACACAAAAGTGACCGTGCACCGTACTATTCAGAGTCTAGGAGCCAAAAGGACGACATTGAGATTAGTAAGAACAAGGACCGAAACTCTAAAAAAGTAGGAGAGGTATTTGGTATTGAAGATAAAGAATCTTCTGGAAAGAAACAAAAGTTGTTTGGAGCTGACAAGGATGCCAATTCTGGAAAAGATGTTGTTATTGTGCTGAAGCAGATGAAAGGAAGACTTCAGGTTCAAAGCTTTCTCATGAGACCAAGCCAGTTTAGGGGCTGTCAGGACCACTGTTTTGATAGTGGCAATAATGTAGATGCTGCAAAAGTGGCAGCAATGAGAGCTGCTGAATTAGTATACACTGCAATTCCTAAAAAGCTTTTATTGGGATGTAGACTTAAGTCTGGCGTTGGTTGGATTCTGTCTGGCCACCGCTGGGATATGGCAGTGTTTGATCCAGAGCGTCAAGAGAAATTCAACAAACTCATGGGTGTGAAAGGAGAAGCCAAAGTGGAGCAGAACTCTGACAATCAAAATGACAACGCTTACTCCTCCGTGCTGAGAAGCAGAAGGAACTTCAGCTGGATCTAG
Protein sequence

>MsG0180002460.01.T01

MPGNKTQNMPEAVVLLDILTDTPPGPPMATLGMTSTQMNIENMRGYHHALHKQDKDREKRATYEEVEKEKRSRDGDARDEKKDSQRSSGEHKSDRAPYYSESRSQKDDIEISKNKDRNSKKVGEVFGIEDKESSGKKQKLFGADKDANSGKDVVIVLKQMKGRLQVQSFLMRPSQFRGCQDHCFDSGNNVDAAKVAAMRAAELVYTAIPKKLLLGCRLKSGVGWILSGHRWDMAVFDPERQEKFNKLMGVKGEAKVEQNSDNQNDNAYSSVLRSRRNFSWI*