AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031361.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031361.01.T02 MTR_6g018970 98.901 91 1 0 10 100 164 254 1.34e-61 188
MsG0680031361.01.T02 MTR_6g018970 98.901 91 1 0 10 100 251 341 2.81e-60 187
MsG0680031361.01.T02 MTR_3g009730 86.813 91 12 0 10 100 263 353 8.37e-54 171
MsG0680031361.01.T02 MTR_3g009730 86.813 91 12 0 10 100 285 375 1.55e-53 171
MsG0680031361.01.T02 MTR_3g009730 86.813 91 12 0 10 100 298 388 5.15e-53 170
MsG0680031361.01.T01 MTR_6g018970 98.901 91 1 0 10 100 164 254 1.34e-61 188
MsG0680031361.01.T01 MTR_6g018970 98.901 91 1 0 10 100 251 341 2.81e-60 187
MsG0680031361.01.T01 MTR_3g009730 86.813 91 12 0 10 100 263 353 8.37e-54 171
MsG0680031361.01.T01 MTR_3g009730 86.813 91 12 0 10 100 285 375 1.55e-53 171
MsG0680031361.01.T01 MTR_3g009730 86.813 91 12 0 10 100 298 388 5.15e-53 170
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031361.01.T02 AT1G13560 83.516 91 15 0 10 100 256 346 2.30e-50 162
MsG0680031361.01.T02 AT1G13560 83.516 91 15 0 10 100 251 341 2.70e-50 162
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 245 337 3.65e-50 161
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 249 341 3.97e-50 161
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 249 341 3.97e-50 161
MsG0680031361.01.T02 AT1G13560 81.720 93 17 0 8 100 297 389 8.35e-50 162
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161
MsG0680031361.01.T02 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161
MsG0680031361.01.T01 AT1G13560 83.516 91 15 0 10 100 256 346 2.30e-50 162
MsG0680031361.01.T01 AT1G13560 83.516 91 15 0 10 100 251 341 2.70e-50 162
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 245 337 3.65e-50 161
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 249 341 3.97e-50 161
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 249 341 3.97e-50 161
MsG0680031361.01.T01 AT1G13560 81.720 93 17 0 8 100 297 389 8.35e-50 162
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161
MsG0680031361.01.T01 AT3G25585 81.720 93 17 0 8 100 297 389 1.05e-49 161

Find 15 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTAATATGAATTTAAA+AGG 0.245275 6:+18912127 MsG0680031361.01.T01:three_prime_UTR
AATCTACCAAGATTTCATTT+AGG 0.298754 6:+18911541 MsG0680031361.01.T01:exon
GAGAACTACTGGCAAGGTTT+TGG 0.326313 6:+18911991 MsG0680031361.01.T01:three_prime_UTR
TACTTGTCTAAAATCACTTT+TGG 0.328559 6:-18911628 None:intergenic
TTATGTATTGATTCTTAGAT+AGG 0.338868 6:+18912047 MsG0680031361.01.T01:three_prime_UTR
GGCATACCTAAATGAAATCT+TGG 0.392925 6:-18911547 None:intergenic
TGTTCCATTGCAGGATAACT+AGG 0.421949 6:+18911507 MsG0680031361.01.T01:intron
CTCGACCAATGAATTAGAAA+AGG 0.434710 6:-18911569 None:intergenic
TATTGATTCTTAGATAGGTT+AGG 0.473920 6:+18912052 MsG0680031361.01.T01:three_prime_UTR
TTGCAAGTCAAGAGAACTAC+TGG 0.491956 6:+18911980 MsG0680031361.01.T01:three_prime_UTR
TCGACCAATGAATTAGAAAA+GGG 0.542526 6:-18911568 None:intergenic
TAACATGAATATATTCATAG+TGG 0.572401 6:-18912105 None:intergenic
TAGGTTAGGAGATTTATTTG+TGG 0.577074 6:+18912066 MsG0680031361.01.T01:three_prime_UTR
CCATTGCAGGATAACTAGGA+AGG 0.604804 6:+18911511 MsG0680031361.01.T01:intron
AGTCAAGAGAACTACTGGCA+AGG 0.657706 6:+18911985 MsG0680031361.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATGTATCTTAAAAAAT+TGG - Chr6:18910118-18910137 None:intergenic 15.0%
!! ATCATGTATCTTAAAAAATT+GGG - Chr6:18910117-18910136 None:intergenic 15.0%
!! CATACATTAAAAATAAAAAG+AGG - Chr6:18912159-18912178 None:intergenic 15.0%
!! TAATATCACTTTAATGAATT+TGG - Chr6:18910400-18910419 None:intergenic 15.0%
!! TAGTCATATAATTTATAATG+AGG - Chr6:18910160-18910179 None:intergenic 15.0%
!! TTGGAAATTTATTAATTCAA+GGG + Chr6:18910880-18910899 MsG0680031361.01.T01:intron 15.0%
!! TTTAGAGAAAAAATTATGTT+TGG - Chr6:18911747-18911766 None:intergenic 15.0%
!!! AGCTTTAATATGAATTTAAA+AGG + Chr6:18912127-18912146 MsG0680031361.01.T01:three_prime_UTR 15.0%
!!! ATACATGATTTTTTCATATT+TGG + Chr6:18910128-18910147 MsG0680031361.01.T01:intron 15.0%
!!! GAAATTGTTTGATATATTTT+TGG + Chr6:18910611-18910630 MsG0680031361.01.T01:intron 15.0%
!! AAGTATATATGTATTCATGT+CGG + Chr6:18911409-18911428 MsG0680031361.01.T01:intron 20.0%
!! CTTGGAAATTTATTAATTCA+AGG + Chr6:18910879-18910898 MsG0680031361.01.T01:intron 20.0%
!! GATAAAATAACATCAATCAA+AGG - Chr6:18910583-18910602 None:intergenic 20.0%
!! TAACATGAATATATTCATAG+TGG - Chr6:18912108-18912127 None:intergenic 20.0%
!!! ATCGATTACATTTATTCTTT+TGG + Chr6:18910784-18910803 MsG0680031361.01.T01:intron 20.0%
!!! GATCATTTTAATTAATTGTG+TGG - Chr6:18910748-18910767 None:intergenic 20.0%
!!! TTATGTATTGATTCTTAGAT+AGG + Chr6:18912047-18912066 MsG0680031361.01.T01:three_prime_UTR 20.0%
!!! TTTTTGTTGAACTTTGTTTT+TGG + Chr6:18911190-18911209 MsG0680031361.01.T01:intron 20.0%
! AAAAGTGAATCAACAAAACA+TGG - Chr6:18911945-18911964 None:intergenic 25.0%
! AATCTACCAAGATTTCATTT+AGG + Chr6:18911541-18911560 MsG0680031361.01.T01:exon 25.0%
! AGAGAAAAAATTATGTTTGG+AGG - Chr6:18911744-18911763 None:intergenic 25.0%
! ATCAAAGGAATTTAGATCAA+AGG - Chr6:18910568-18910587 None:intergenic 25.0%
! ATGAATTCTAGCACAAAATT+AGG + Chr6:18911704-18911723 MsG0680031361.01.T01:intron 25.0%
! CATAAATTCAACTCATGTAT+TGG - Chr6:18910200-18910219 None:intergenic 25.0%
! CTAAATTAGTGTCTAATGTA+TGG + Chr6:18911145-18911164 MsG0680031361.01.T01:intron 25.0%
! TATGATTCCATCTTCAAAAA+AGG + Chr6:18912175-18912194 MsG0680031361.01.T01:three_prime_UTR 25.0%
! TGCAATATCAATGATTAAAC+CGG - Chr6:18910707-18910726 None:intergenic 25.0%
! TTCTTATGACCTATATAATG+CGG - Chr6:18911456-18911475 None:intergenic 25.0%
! TTTAGATAAACTTATGTGTG+AGG - Chr6:18911361-18911380 None:intergenic 25.0%
!! ATCGATGATTTTCAATTTCA+TGG - Chr6:18910770-18910789 None:intergenic 25.0%
!! TCAATACTAATTTTGCTACA+AGG + Chr6:18912014-18912033 MsG0680031361.01.T01:three_prime_UTR 25.0%
!!! AACTTTGTTTTTGGAAAAGT+TGG + Chr6:18911199-18911218 MsG0680031361.01.T01:intron 25.0%
!!! GATGTTATTTTATCTTCTCA+TGG + Chr6:18910589-18910608 MsG0680031361.01.T01:intron 25.0%
!!! TACTTGTCTAAAATCACTTT+TGG - Chr6:18911631-18911650 None:intergenic 25.0%
!!! TATTGATTCTTAGATAGGTT+AGG + Chr6:18912052-18912071 MsG0680031361.01.T01:three_prime_UTR 25.0%
!!! TGTTTGATATATTTTTGGCA+GGG + Chr6:18910616-18910635 MsG0680031361.01.T01:intron 25.0%
!!! TTGTTTGATATATTTTTGGC+AGG + Chr6:18910615-18910634 MsG0680031361.01.T01:intron 25.0%
CTATGTTATCCGCATTATAT+AGG + Chr6:18911444-18911463 MsG0680031361.01.T01:intron 30.0%
GGTTTAATCATTGATATTGC+AGG + Chr6:18910706-18910725 MsG0680031361.01.T01:intron 30.0%
TAGGTTAGGAGATTTATTTG+TGG + Chr6:18912066-18912085 MsG0680031361.01.T01:three_prime_UTR 30.0%
TATCAATGATTAAACCGGTA+CGG - Chr6:18910702-18910721 None:intergenic 30.0%
TCGACCAATGAATTAGAAAA+GGG - Chr6:18911571-18911590 None:intergenic 30.0%
TTCCATCTTCAAAAAAGGTA+TGG + Chr6:18912180-18912199 MsG0680031361.01.T01:three_prime_UTR 30.0%
! ACTAGTCTCTCATTAACTAA+AGG - Chr6:18910644-18910663 None:intergenic 30.0%
! TATGCCCTTTTCTAATTCAT+TGG + Chr6:18911564-18911583 MsG0680031361.01.T01:intron 30.0%
!! ATTCCAGTTTTTAAGCCTTT+TGG - Chr6:18910056-18910075 None:intergenic 30.0%
!! TTTAGTTCTTCGTATTGTAG+AGG - Chr6:18910920-18910939 None:intergenic 30.0%
!!! AACCATACCTTTTTTGAAGA+TGG - Chr6:18912185-18912204 None:intergenic 30.0%
!!! ACGTGTCAAAGGTTTTTTTT+AGG - Chr6:18911328-18911347 None:intergenic 30.0%
AAAAAACCTTTGACACGTCT+TGG + Chr6:18911330-18911349 MsG0680031361.01.T01:intron 35.0%
AATCAACAAAACATGGACAG+AGG - Chr6:18911938-18911957 None:intergenic 35.0%
CACTTATGTGATGAACCAAA+AGG + Chr6:18910038-18910057 MsG0680031361.01.T01:CDS 35.0%
CTCGACCAATGAATTAGAAA+AGG - Chr6:18911572-18911591 None:intergenic 35.0%
CTTAAAAACTGGAATGTGCA+TGG + Chr6:18910061-18910080 MsG0680031361.01.T01:CDS 35.0%
GAACCAAAAGGCTTAAAAAC+TGG + Chr6:18910050-18910069 MsG0680031361.01.T01:CDS 35.0%
GGCATACCTAAATGAAATCT+TGG - Chr6:18911550-18911569 None:intergenic 35.0%
TTATCCTGCAATGGAACAAT+AGG - Chr6:18911505-18911524 None:intergenic 35.0%
TTTCAGTTTCAACGATGTCT+TGG + Chr6:18910861-18910880 MsG0680031361.01.T01:intron 35.0%
TTTGCAGAAACAGAATCATC+AGG - Chr6:18911032-18911051 None:intergenic 35.0%
! AATGAGAGACTAGTTCTTCT+TGG + Chr6:18910650-18910669 MsG0680031361.01.T01:CDS 35.0%
! CTTTTCTAATTCATTGGTCG+AGG + Chr6:18911570-18911589 MsG0680031361.01.T01:intron 35.0%
! GAGGTTATTCGACATTTTCT+TGG + Chr6:18911589-18911608 MsG0680031361.01.T01:intron 35.0%
! GATAGAAGCTCAATTTTGCA+GGG + Chr6:18909999-18910018 MsG0680031361.01.T01:CDS 35.0%
! TGATAGAAGCTCAATTTTGC+AGG + Chr6:18909998-18910017 MsG0680031361.01.T01:CDS 35.0%
!! GGACAATTGTCTGATTTGTT+CGG - Chr6:18911307-18911326 None:intergenic 35.0%
ACCTGAAGCAATATATTCCC+AGG - Chr6:18911112-18911131 None:intergenic 40.0%
CAAAACATGGACAGAGGAAA+TGG - Chr6:18911932-18911951 None:intergenic 40.0%
CATGAAATTACGAATGCCCT+GGG + Chr6:18911092-18911111 MsG0680031361.01.T01:CDS 40.0%
CCTGAAGCAATATATTCCCA+GGG - Chr6:18911111-18911130 None:intergenic 40.0%
CTTGCATCAAGGCTAAATGA+CGG + Chr6:18910513-18910532 MsG0680031361.01.T01:CDS 40.0%
CTTGGTTACTGTGCATTTAC+AGG + Chr6:18910668-18910687 MsG0680031361.01.T01:CDS 40.0%
CTTTCCTATTGTTCCATTGC+AGG + Chr6:18911498-18911517 MsG0680031361.01.T01:intron 40.0%
TACAGGTACATATACCGTAC+CGG + Chr6:18910685-18910704 MsG0680031361.01.T01:intron 40.0%
TCATGAAATTACGAATGCCC+TGG + Chr6:18911091-18911110 MsG0680031361.01.T01:CDS 40.0%
TGACTACACTTCGATCACAT+AGG + Chr6:18910174-18910193 MsG0680031361.01.T01:intron 40.0%
TGATGCAAGTGCATTAGCAA+TGG - Chr6:18910502-18910521 None:intergenic 40.0%
TGCGGATAACATAGACGATA+CGG - Chr6:18911438-18911457 None:intergenic 40.0%
TGTTCCATTGCAGGATAACT+AGG + Chr6:18911507-18911526 MsG0680031361.01.T01:intron 40.0%
TTGCAAGTCAAGAGAACTAC+TGG + Chr6:18911980-18911999 MsG0680031361.01.T01:three_prime_UTR 40.0%
TTGCATCAAGGCTAAATGAC+GGG + Chr6:18910514-18910533 MsG0680031361.01.T01:CDS 40.0%
TTGCTAATGCACTTGCATCA+AGG + Chr6:18910502-18910521 MsG0680031361.01.T01:CDS 40.0%
!! GAGGAAATGGATTGTGTGTT+TGG - Chr6:18911919-18911938 None:intergenic 40.0%
!! GGATTGTGTGTTTGGTTCTA+CGG - Chr6:18911911-18911930 None:intergenic 40.0%
AGTCAAGAGAACTACTGGCA+AGG + Chr6:18911985-18912004 MsG0680031361.01.T01:three_prime_UTR 45.0%
AGTGCATTAGCAATGGCAAG+TGG - Chr6:18910495-18910514 None:intergenic 45.0%
CAGAAACAGAATCATCAGGC+AGG - Chr6:18911028-18911047 None:intergenic 45.0%
CCATTGCAGGATAACTAGGA+AGG + Chr6:18911511-18911530 MsG0680031361.01.T01:intron 45.0%
CCCTGGGAATATATTGCTTC+AGG + Chr6:18911108-18911127 MsG0680031361.01.T01:CDS 45.0%
CCTTCCTAGTTATCCTGCAA+TGG - Chr6:18911514-18911533 None:intergenic 45.0%
GGCAAGTGGTAGATACATGA+GGG - Chr6:18910481-18910500 None:intergenic 45.0%
TGGCAAGTGGTAGATACATG+AGG - Chr6:18910482-18910501 None:intergenic 45.0%
! GAAGCTCAATTTTGCAGGGA+AGG + Chr6:18910003-18910022 MsG0680031361.01.T01:CDS 45.0%
! GAGAACTACTGGCAAGGTTT+TGG + Chr6:18911991-18912010 MsG0680031361.01.T01:three_prime_UTR 45.0%
! TAACAAAGCCTTTTCGCGCT+AGG - Chr6:18910252-18910271 None:intergenic 45.0%
CCAACCGGACACATAGCTTA+GGG - Chr6:18911235-18911254 None:intergenic 50.0%
CTTGCCCTAAGCTATGTGTC+CGG + Chr6:18911228-18911247 MsG0680031361.01.T01:intron 50.0%
GAGTGTCCAAGACGTGTCAA+AGG - Chr6:18911339-18911358 None:intergenic 50.0%
GCTAGGTGGAGTCAGTTTCA+TGG - Chr6:18910235-18910254 None:intergenic 50.0%
ACCGACGCAAGTTTGCCAAC+CGG - Chr6:18911250-18911269 None:intergenic 55.0%
CCCTAAGCTATGTGTCCGGT+TGG + Chr6:18911232-18911251 MsG0680031361.01.T01:intron 55.0%
GCCAACCGGACACATAGCTT+AGG - Chr6:18911236-18911255 None:intergenic 55.0%
TCCGGTTGGCAAACTTGCGT+CGG + Chr6:18911246-18911265 MsG0680031361.01.T01:intron 55.0%
TGACTCCACCTAGCGCGAAA+AGG + Chr6:18910241-18910260 MsG0680031361.01.T01:intron 55.0%
! CAAAGCCTTTTCGCGCTAGG+TGG - Chr6:18910249-18910268 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 18909987 18912208 18909987 ID=MsG0680031361.01;Name=MsG0680031361.01
Chr6 mRNA 18909987 18912208 18909987 ID=MsG0680031361.01.T01;Parent=MsG0680031361.01;Name=MsG0680031361.01.T01;_AED=0.47;_eAED=0.50;_QI=0|1|0.83|1|0.4|0.16|6|251|100
Chr6 exon 18911520 18911562 18911520 ID=MsG0680031361.01.T01:exon:12590;Parent=MsG0680031361.01.T01
Chr6 exon 18911979 18912208 18911979 ID=MsG0680031361.01.T01:exon:12591;Parent=MsG0680031361.01.T01
Chr6 CDS 18909987 18910082 18909987 ID=MsG0680031361.01.T01:cds;Parent=MsG0680031361.01.T01
Chr6 CDS 18910477 18910535 18910477 ID=MsG0680031361.01.T01:cds;Parent=MsG0680031361.01.T01
Chr6 CDS 18910637 18910689 18910637 ID=MsG0680031361.01.T01:cds;Parent=MsG0680031361.01.T01
Chr6 CDS 18911057 18911129 18911057 ID=MsG0680031361.01.T01:cds;Parent=MsG0680031361.01.T01
Chr6 CDS 18911520 18911541 18911520 ID=MsG0680031361.01.T01:cds;Parent=MsG0680031361.01.T01
Chr6 three_prime_UTR 18911542 18911562 18911542 ID=MsG0680031361.01.T01:three_prime_utr;Parent=MsG0680031361.01.T01
Chr6 three_prime_UTR 18911979 18912208 18911979 ID=MsG0680031361.01.T01:three_prime_utr;Parent=MsG0680031361.01.T01
Chr6 mRNA 18909987 18911712 18909987 ID=MsG0680031361.01.T02;Parent=MsG0680031361.01;Name=MsG0680031361.01.T02;_AED=0.48;_eAED=0.51;_QI=0|1|0.8|1|0.5|0.2|5|171|100
Chr6 exon 18911520 18911712 18911520 ID=MsG0680031361.01.T02:exon:12592;Parent=MsG0680031361.01.T02
Chr6 CDS 18909987 18910082 18909987 ID=MsG0680031361.01.T02:cds;Parent=MsG0680031361.01.T02
Chr6 CDS 18910477 18910535 18910477 ID=MsG0680031361.01.T02:cds;Parent=MsG0680031361.01.T02
Chr6 CDS 18910637 18910689 18910637 ID=MsG0680031361.01.T02:cds;Parent=MsG0680031361.01.T02
Chr6 CDS 18911057 18911129 18911057 ID=MsG0680031361.01.T02:cds;Parent=MsG0680031361.01.T02
Chr6 CDS 18911520 18911541 18911520 ID=MsG0680031361.01.T02:cds;Parent=MsG0680031361.01.T02
Chr6 three_prime_UTR 18911542 18911712 18911542 ID=MsG0680031361.01.T02:three_prime_utr;Parent=MsG0680031361.01.T02
Gene Sequence

>MsG0680031361.01.T02

ATGACTTTTACTGATAGAAGCTCAATTTTGCAGGGAAGGATGATTTTGTCTCACTTATGTGATGAACCAAAAGGCTTAAAAACTGGAATGTGCATGTCCCTCATGTATCTACCACTTGCCATTGCTAATGCACTTGCATCAAGGCTAAATGACGGGGTTCCTTTAGTTAATGAGAGACTAGTTCTTCTTGGTTACTGTGCATTTACAGCATCACTTTACTTGCATTTCGCGACATCTGTCATTCATGAAATTACGAATGCCCTGGGAATATATTGCTTCAGGATAACTAGGAAGGAAGCTTAA

>MsG0680031361.01.T01

ATGACTTTTACTGATAGAAGCTCAATTTTGCAGGGAAGGATGATTTTGTCTCACTTATGTGATGAACCAAAAGGCTTAAAAACTGGAATGTGCATGTCCCTCATGTATCTACCACTTGCCATTGCTAATGCACTTGCATCAAGGCTAAATGACGGGGTTCCTTTAGTTAATGAGAGACTAGTTCTTCTTGGTTACTGTGCATTTACAGCATCACTTTACTTGCATTTCGCGACATCTGTCATTCATGAAATTACGAATGCCCTGGGAATATATTGCTTCAGGATAACTAGGAAGGAAGCTTAA

Protein sequence

>MsG0680031361.01.T02

MTFTDRSSILQGRMILSHLCDEPKGLKTGMCMSLMYLPLAIANALASRLNDGVPLVNERLVLLGYCAFTASLYLHFATSVIHEITNALGIYCFRITRKEA*

>MsG0680031361.01.T01

MTFTDRSSILQGRMILSHLCDEPKGLKTGMCMSLMYLPLAIANALASRLNDGVPLVNERLVLLGYCAFTASLYLHFATSVIHEITNALGIYCFRITRKEA*