Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005435.01.T02 | XP_003592284.1 | 100 | 73 | 0 | 0 | 1 | 73 | 377 | 449 | 1.56E-41 | 150 |
| MsG0180005435.01.T03 | XP_003592284.1 | 100 | 62 | 0 | 0 | 1 | 62 | 388 | 449 | 5.06E-33 | 127 |
| MsG0180005435.01.T04 | XP_003592284.1 | 100 | 47 | 0 | 0 | 1 | 47 | 403 | 449 | 7.53E-20 | 91.3 |
| MsG0180005435.01.T01 | MCI26468.1 | 96.552 | 87 | 3 | 0 | 1 | 87 | 43 | 129 | 2.20E-52 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005435.01.T01 | Q41783 | 96.25 | 80 | 1 | 1 | 1 | 80 | 363 | 440 | 5.91E-46 | 155 |
| MsG0180005435.01.T02 | Q13509 | 89.552 | 67 | 7 | 0 | 1 | 67 | 377 | 443 | 1.16E-35 | 127 |
| MsG0180005435.01.T03 | P09652 | 92.857 | 56 | 4 | 0 | 1 | 56 | 388 | 443 | 1.87E-28 | 107 |
| MsG0180005435.01.T04 | Q13509 | 90.244 | 41 | 4 | 0 | 1 | 41 | 403 | 443 | 2.24E-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005435.01.T01 | A0A5B7BMR8 | 98.824 | 85 | 1 | 0 | 1 | 85 | 217 | 301 | 9.09e-53 | 176 |
| MsG0180005435.01.T02 | G7IAN2 | 100.000 | 73 | 0 | 0 | 1 | 73 | 377 | 449 | 7.47e-42 | 150 |
| MsG0180005435.01.T03 | G7IAN2 | 100.000 | 62 | 0 | 0 | 1 | 62 | 388 | 449 | 2.42e-33 | 127 |
| MsG0180005435.01.T04 | G7IAN2 | 100.000 | 47 | 0 | 0 | 1 | 47 | 403 | 449 | 3.60e-20 | 91.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048258.01 | MsG0180005435.01 | 0.800682 | 1.285475e-48 | 6.022504e-46 |
| MsG0080048311.01 | MsG0180005435.01 | 0.808181 | 3.514138e-50 | 1.995844e-47 |
| MsG0080048701.01 | MsG0180005435.01 | 0.806822 | 6.825172e-50 | 3.741247e-47 |
| MsG0080048781.01 | MsG0180005435.01 | 0.851184 | 1.046657e-60 | 2.101262e-57 |
| MsG0080049140.01 | MsG0180005435.01 | 0.843165 | 1.657445e-58 | 2.570683e-55 |
| MsG0180000111.01 | MsG0180005435.01 | 0.827838 | 1.254259e-54 | 1.224977e-51 |
| MsG0180000314.01 | MsG0180005435.01 | 0.812774 | 3.579707e-51 | 2.296141e-48 |
| MsG0180000373.01 | MsG0180005435.01 | 0.823717 | 1.191873e-53 | 1.034524e-50 |
| MsG0180000601.01 | MsG0180005435.01 | 0.800622 | 1.322182e-48 | 6.185164e-46 |
| MsG0180002211.01 | MsG0180005435.01 | 0.803067 | 4.160139e-49 | 2.070623e-46 |
| MsG0180002421.01 | MsG0180005435.01 | 0.827195 | 1.789405e-54 | 1.715355e-51 |
| MsG0180002583.01 | MsG0180005435.01 | 0.810208 | 1.292449e-50 | 7.743292e-48 |
| MsG0180003427.01 | MsG0180005435.01 | 0.833482 | 5.200978e-56 | 5.997218e-53 |
| MsG0180003485.01 | MsG0180005435.01 | 0.843862 | 1.079149e-58 | 1.711195e-55 |
| MsG0180003796.01 | MsG0180005435.01 | 0.872815 | 2.378973e-67 | 1.009274e-63 |
| MsG0180003994.01 | MsG0180005435.01 | 0.809923 | 1.488066e-50 | 8.849911e-48 |
| MsG0180004126.01 | MsG0180005435.01 | 0.808912 | 2.452919e-50 | 1.420009e-47 |
| MsG0180004661.01 | MsG0180005435.01 | 0.812022 | 5.225066e-51 | 3.284961e-48 |
| MsG0180004711.01 | MsG0180005435.01 | 0.808503 | 2.999199e-50 | 1.717824e-47 |
| MsG0180004806.01 | MsG0180005435.01 | 0.808266 | 3.370059e-50 | 1.918339e-47 |
| MsG0180005002.01 | MsG0180005435.01 | 0.871262 | 7.816570e-67 | 3.133041e-63 |
| MsG0180005435.01 | MsG0180005835.01 | 0.836237 | 1.052512e-56 | 1.318630e-53 |
| MsG0180005435.01 | MsG0180006133.01 | 0.836357 | 9.810445e-57 | 1.233415e-53 |
| MsG0180005435.01 | MsG0280006311.01 | 0.833939 | 3.996844e-56 | 4.673091e-53 |
| MsG0180005435.01 | MsG0280006477.01 | 0.813039 | 3.130999e-51 | 2.022626e-48 |
| MsG0180005435.01 | MsG0280006540.01 | 0.855307 | 6.885800e-62 | 1.583017e-58 |
| MsG0180005435.01 | MsG0280006563.01 | 0.808173 | 3.527054e-50 | 2.002807e-47 |
| MsG0180005435.01 | MsG0280007359.01 | 0.852522 | 4.367369e-61 | 9.150343e-58 |
| MsG0180005435.01 | MsG0280008280.01 | 0.801820 | 7.516059e-49 | 3.624304e-46 |
| MsG0180005435.01 | MsG0280008679.01 | 0.819498 | 1.125443e-52 | 8.675339e-50 |
| MsG0180005435.01 | MsG0280009752.01 | 0.825892 | 3.658162e-54 | 3.377924e-51 |
| MsG0180005435.01 | MsG0280010779.01 | 0.818152 | 2.275751e-52 | 1.690061e-49 |
| MsG0180005435.01 | MsG0280010795.01 | 0.836263 | 1.036728e-56 | 1.299818e-53 |
| MsG0180005435.01 | MsG0280011154.01 | 0.809956 | 1.464217e-50 | 8.715708e-48 |
| MsG0180005435.01 | MsG0380012788.01 | 0.811192 | 7.915554e-51 | 4.868060e-48 |
| MsG0180005435.01 | MsG0380012808.01 | 0.802894 | 4.517969e-49 | 2.238983e-46 |
| MsG0180005435.01 | MsG0380014387.01 | 0.809469 | 1.863016e-50 | 1.094737e-47 |
| MsG0180005435.01 | MsG0380014754.01 | 0.809406 | 1.921963e-50 | 1.127530e-47 |
| MsG0180005435.01 | MsG0380014808.01 | 0.871855 | 4.970322e-67 | 2.035053e-63 |
| MsG0180005435.01 | MsG0380015161.01 | 0.827049 | 1.938963e-54 | 1.850660e-51 |
| MsG0180005435.01 | MsG0380015548.01 | 0.861102 | 1.298614e-63 | 3.631803e-60 |
| MsG0180005435.01 | MsG0380015816.01 | 0.811359 | 7.281692e-51 | 4.497822e-48 |
| MsG0180005435.01 | MsG0380016140.01 | 0.809699 | 1.663376e-50 | 9.834182e-48 |
| MsG0180005435.01 | MsG0380016225.01 | 0.816940 | 4.268398e-52 | 3.066536e-49 |
| MsG0180005435.01 | MsG0380016703.01 | 0.827046 | 1.941655e-54 | 1.853048e-51 |
| MsG0180005435.01 | MsG0380017159.01 | 0.824854 | 6.442366e-54 | 5.775481e-51 |
| MsG0180005435.01 | MsG0380017164.01 | 0.811973 | 5.354760e-51 | 3.362056e-48 |
| MsG0180005435.01 | MsG0380017239.01 | 0.807737 | 4.367508e-50 | 2.451689e-47 |
| MsG0180005435.01 | MsG0380017361.01 | 0.845753 | 3.336766e-59 | 5.618112e-56 |
| MsG0180005435.01 | MsG0480018397.01 | 0.850447 | 1.688480e-60 | 3.308465e-57 |
| MsG0180005435.01 | MsG0480018526.01 | 0.861558 | 9.431461e-64 | 2.679373e-60 |
| MsG0180005435.01 | MsG0480018785.01 | 0.818803 | 1.620202e-52 | 1.224995e-49 |
| MsG0180005435.01 | MsG0480018919.01 | 0.804860 | 1.763995e-49 | 9.195313e-47 |
| MsG0180005435.01 | MsG0480019635.01 | 0.825555 | 4.396997e-54 | 4.021103e-51 |
| MsG0180005435.01 | MsG0480019699.01 | 0.801439 | 8.999467e-49 | 4.297902e-46 |
| MsG0180005435.01 | MsG0480020713.01 | 0.812452 | 4.210125e-51 | 2.678058e-48 |
| MsG0180005435.01 | MsG0480020965.01 | 0.832094 | 1.149856e-55 | 1.272184e-52 |
| MsG0180005435.01 | MsG0480021013.01 | 0.829365 | 5.362745e-55 | 5.477425e-52 |
| MsG0180005435.01 | MsG0480021156.01 | 0.822638 | 2.128049e-53 | 1.791487e-50 |
| MsG0180005435.01 | MsG0480022325.01 | 0.819852 | 9.340954e-53 | 7.271692e-50 |
| MsG0180005435.01 | MsG0480022331.01 | 0.815699 | 8.088250e-52 | 5.616511e-49 |
| MsG0180005435.01 | MsG0480022982.01 | 0.809711 | 1.653530e-50 | 9.779189e-48 |
| MsG0180005435.01 | MsG0480022998.01 | 0.814860 | 1.242640e-51 | 8.433167e-49 |
| MsG0180005435.01 | MsG0480023262.01 | 0.838278 | 3.161795e-57 | 4.213826e-54 |
| MsG0180005435.01 | MsG0480023296.01 | 0.836275 | 1.029269e-56 | 1.290961e-53 |
| MsG0180005435.01 | MsG0480023458.01 | 0.819446 | 1.156265e-52 | 8.899933e-50 |
| MsG0180005435.01 | MsG0480023653.01 | 0.816005 | 6.911687e-52 | 4.839856e-49 |
| MsG0180005435.01 | MsG0480023672.01 | 0.837898 | 3.960130e-57 | 5.217422e-54 |
| MsG0180005435.01 | MsG0580024489.01 | 0.833764 | 4.420393e-56 | 5.140781e-53 |
| MsG0180005435.01 | MsG0580024657.01 | 0.815732 | 7.952782e-52 | 5.527754e-49 |
| MsG0180005435.01 | MsG0580024748.01 | 0.805526 | 1.279134e-49 | 6.782510e-47 |
| MsG0180005435.01 | MsG0580024758.01 | 0.801305 | 9.585953e-49 | 4.562591e-46 |
| MsG0180005435.01 | MsG0580024787.01 | 0.807606 | 4.655485e-50 | 2.604295e-47 |
| MsG0180005435.01 | MsG0580024813.01 | 0.819908 | 9.073462e-53 | 7.074555e-50 |
| MsG0180005435.01 | MsG0580024954.01 | 0.848529 | 5.784488e-60 | 1.064704e-56 |
| MsG0180005435.01 | MsG0580025203.01 | 0.834232 | 3.376942e-56 | 3.982455e-53 |
| MsG0180005435.01 | MsG0580025506.01 | 0.804343 | 2.261375e-49 | 1.163119e-46 |
| MsG0180005435.01 | MsG0580025515.01 | 0.816984 | 4.171979e-52 | 3.000922e-49 |
| MsG0180005435.01 | MsG0580025614.01 | 0.811166 | 8.021736e-51 | 4.929811e-48 |
| MsG0180005435.01 | MsG0580026142.01 | 0.813782 | 2.150175e-51 | 1.417292e-48 |
| MsG0180005435.01 | MsG0580028756.01 | 0.852899 | 3.410659e-61 | 7.235773e-58 |
| MsG0180005435.01 | MsG0580028923.01 | 0.815252 | 1.016852e-51 | 6.974578e-49 |
| MsG0180005435.01 | MsG0580029261.01 | 0.816618 | 5.039096e-52 | 3.588272e-49 |
| MsG0180005435.01 | MsG0580029298.01 | 0.829515 | 4.930604e-55 | 5.057554e-52 |
| MsG0180005435.01 | MsG0580029591.01 | 0.848243 | 6.941284e-60 | 1.265759e-56 |
| MsG0180005435.01 | MsG0680031616.01 | 0.821792 | 3.343831e-53 | 2.747762e-50 |
| MsG0180005435.01 | MsG0680031701.01 | 0.836801 | 7.560968e-57 | 9.633225e-54 |
| MsG0180005435.01 | MsG0680032773.01 | 0.821207 | 4.565040e-53 | 3.690341e-50 |
| MsG0180005435.01 | MsG0680033696.01 | 0.809238 | 2.089024e-50 | 1.220045e-47 |
| MsG0180005435.01 | MsG0680034598.01 | 0.821755 | 3.409940e-53 | 2.799031e-50 |
| MsG0180005435.01 | MsG0780039578.01 | 0.818752 | 1.663320e-52 | 1.255906e-49 |
| MsG0180005435.01 | MsG0780039861.01 | 0.854852 | 9.341112e-62 | 2.115068e-58 |
| MsG0180005435.01 | MsG0780039878.01 | 0.808183 | 3.509314e-50 | 1.993219e-47 |
| MsG0180005435.01 | MsG0780040307.01 | 0.806053 | 9.914698e-50 | 5.328937e-47 |
| MsG0180005435.01 | MsG0780040364.01 | 0.850554 | 1.575217e-60 | 3.097228e-57 |
| MsG0180005435.01 | MsG0780040533.01 | 0.809600 | 1.746542e-50 | 1.029932e-47 |
| MsG0180005435.01 | MsG0780040620.01 | 0.826367 | 2.820826e-54 | 2.640128e-51 |
| MsG0180005435.01 | MsG0780040896.01 | 0.818966 | 1.487675e-52 | 1.130057e-49 |
| MsG0180005435.01 | MsG0780041063.01 | 0.832798 | 7.695198e-56 | 8.693670e-53 |
| MsG0180005435.01 | MsG0780041132.01 | 0.838054 | 3.612271e-57 | 4.782030e-54 |
| MsG0180005435.01 | MsG0780041143.01 | 0.865676 | 4.969760e-65 | 1.630896e-61 |
| MsG0180005435.01 | MsG0780041756.01 | 0.831892 | 1.289904e-55 | 1.418975e-52 |
| MsG0180005435.01 | MsG0880042927.01 | 0.802466 | 5.537249e-49 | 2.714352e-46 |
| MsG0180005435.01 | MsG0880043516.01 | 0.828637 | 8.049023e-55 | 8.046685e-52 |
| MsG0180005435.01 | MsG0880043614.01 | 0.822415 | 2.397724e-53 | 2.005664e-50 |
| MsG0180005435.01 | MsG0880043637.01 | 0.804275 | 2.335461e-49 | 1.199127e-46 |
| MsG0180005435.01 | MsG0880044989.01 | 0.817214 | 3.703458e-52 | 2.680556e-49 |
| MsG0180005435.01 | MsG0880045552.01 | 0.811909 | 5.528669e-51 | 3.465131e-48 |
| MsG0180005435.01 | MsG0880045612.01 | 0.801005 | 1.104051e-48 | 5.215737e-46 |
| MsG0180005435.01 | MsG0880046208.01 | 0.839262 | 1.759832e-57 | 2.417109e-54 |
| MsG0180005435.01 | MsG0880046553.01 | 0.817481 | 3.225261e-52 | 2.351621e-49 |
| MsG0180005435.01 | MsG0880046646.01 | 0.802165 | 6.386753e-49 | 3.106829e-46 |
| MsG0180005435.01 | MsG0880046885.01 | 0.802755 | 4.825566e-49 | 2.383043e-46 |
| MsG0180005435.01 | MsG0880046905.01 | 0.806710 | 7.206331e-50 | 3.938720e-47 |
| MsG0180005435.01 | MsG0880046991.01 | 0.823200 | 1.574186e-53 | 1.346350e-50 |
| MsG0180005435.01 | MsG0880047223.01 | 0.805352 | 1.391593e-49 | 7.345895e-47 |
| MsG0180005435.01 | MsG0880047226.01 | 0.838402 | 2.938420e-57 | 3.931376e-54 |
| MsG0180005435.01 | MsG0880047431.01 | 0.800143 | 1.655255e-48 | 7.649730e-46 |
| MsG0180005435.01 | MsG0880047466.01 | 0.832259 | 1.046729e-55 | 1.163619e-52 |
| MsG0180005435.01 | MsG0880047649.01 | 0.804627 | 1.972720e-49 | 1.022087e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005435.01.T02 | MTR_1g101130 | 100.000 | 73 | 0 | 0 | 1 | 73 | 377 | 449 | 1.89e-45 | 150 |
| MsG0180005435.01.T02 | MTR_8g107250 | 84.848 | 66 | 10 | 0 | 1 | 66 | 378 | 443 | 5.26e-34 | 120 |
| MsG0180005435.01.T02 | MTR_4g019090 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 9.89e-33 | 117 |
| MsG0180005435.01.T02 | MTR_4g019110 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.05e-32 | 117 |
| MsG0180005435.01.T02 | MTR_7g089120 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.21e-32 | 116 |
| MsG0180005435.01.T02 | MTR_4g094535 | 98.148 | 54 | 1 | 0 | 1 | 54 | 377 | 430 | 2.91e-32 | 115 |
| MsG0180005435.01.T02 | MTR_2g031310 | 98.148 | 54 | 1 | 0 | 1 | 54 | 377 | 430 | 2.94e-32 | 115 |
| MsG0180005435.01.T02 | MTR_4g017630 | 98.148 | 54 | 1 | 0 | 1 | 54 | 377 | 430 | 3.40e-32 | 115 |
| MsG0180005435.01.T02 | MTR_3g113970 | 96.296 | 54 | 2 | 0 | 1 | 54 | 379 | 432 | 1.32e-31 | 114 |
| MsG0180005435.01.T02 | MTR_8g098360 | 90.741 | 54 | 5 | 0 | 1 | 54 | 377 | 430 | 1.76e-29 | 108 |
| MsG0180005435.01.T02 | MTR_4g093670 | 82.759 | 29 | 5 | 0 | 1 | 29 | 1 | 29 | 7.36e-13 | 57.4 |
| MsG0180005435.01.T02 | MTR_6g029190 | 39.344 | 61 | 37 | 0 | 1 | 61 | 388 | 448 | 3.69e-11 | 57.0 |
| MsG0180005435.01.T03 | MTR_1g101130 | 100.000 | 62 | 0 | 0 | 1 | 62 | 388 | 449 | 6.13e-37 | 127 |
| MsG0180005435.01.T03 | MTR_8g107250 | 83.636 | 55 | 9 | 0 | 1 | 55 | 389 | 443 | 2.77e-26 | 99.0 |
| MsG0180005435.01.T03 | MTR_4g019090 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 9.20e-25 | 94.7 |
| MsG0180005435.01.T03 | MTR_4g019110 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 9.87e-25 | 94.7 |
| MsG0180005435.01.T03 | MTR_7g089120 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.01e-24 | 94.7 |
| MsG0180005435.01.T03 | MTR_2g031310 | 97.674 | 43 | 1 | 0 | 1 | 43 | 388 | 430 | 2.72e-24 | 93.6 |
| MsG0180005435.01.T03 | MTR_4g094535 | 97.674 | 43 | 1 | 0 | 1 | 43 | 388 | 430 | 2.76e-24 | 93.2 |
| MsG0180005435.01.T03 | MTR_4g017630 | 97.674 | 43 | 1 | 0 | 1 | 43 | 388 | 430 | 3.16e-24 | 93.2 |
| MsG0180005435.01.T03 | MTR_3g113970 | 95.349 | 43 | 2 | 0 | 1 | 43 | 390 | 432 | 1.17e-23 | 91.7 |
| MsG0180005435.01.T03 | MTR_8g098360 | 90.698 | 43 | 4 | 0 | 1 | 43 | 388 | 430 | 4.69e-22 | 87.0 |
| MsG0180005435.01.T04 | MTR_1g101130 | 100.000 | 47 | 0 | 0 | 1 | 47 | 403 | 449 | 9.12e-24 | 91.3 |
| MsG0180005435.01.T04 | MTR_8g107250 | 77.500 | 40 | 9 | 0 | 1 | 40 | 404 | 443 | 3.04e-13 | 61.6 |
| MsG0180005435.01.T04 | MTR_4g019090 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 2.75e-12 | 58.9 |
| MsG0180005435.01.T04 | MTR_7g089120 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 2.88e-12 | 58.9 |
| MsG0180005435.01.T04 | MTR_4g019110 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 2.94e-12 | 58.9 |
| MsG0180005435.01.T04 | MTR_2g031310 | 96.429 | 28 | 1 | 0 | 1 | 28 | 403 | 430 | 7.82e-12 | 57.8 |
| MsG0180005435.01.T04 | MTR_4g094535 | 96.429 | 28 | 1 | 0 | 1 | 28 | 403 | 430 | 8.45e-12 | 57.4 |
| MsG0180005435.01.T04 | MTR_4g017630 | 96.429 | 28 | 1 | 0 | 1 | 28 | 403 | 430 | 8.79e-12 | 57.4 |
| MsG0180005435.01.T04 | MTR_3g113970 | 92.857 | 28 | 2 | 0 | 1 | 28 | 405 | 432 | 3.56e-11 | 55.8 |
| MsG0180005435.01.T04 | MTR_8g098360 | 92.857 | 28 | 2 | 0 | 1 | 28 | 403 | 430 | 5.27e-11 | 55.5 |
| MsG0180005435.01.T01 | MTR_1g101130 | 100.000 | 87 | 0 | 0 | 1 | 87 | 363 | 449 | 4.69e-56 | 179 |
| MsG0180005435.01.T01 | MTR_8g107250 | 86.250 | 80 | 11 | 0 | 1 | 80 | 364 | 443 | 3.23e-44 | 148 |
| MsG0180005435.01.T01 | MTR_4g019090 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 7.34e-43 | 144 |
| MsG0180005435.01.T01 | MTR_4g019110 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 8.33e-43 | 144 |
| MsG0180005435.01.T01 | MTR_7g089120 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 8.42e-43 | 144 |
| MsG0180005435.01.T01 | MTR_2g031310 | 98.529 | 68 | 1 | 0 | 1 | 68 | 363 | 430 | 1.91e-42 | 143 |
| MsG0180005435.01.T01 | MTR_4g017630 | 98.529 | 68 | 1 | 0 | 1 | 68 | 363 | 430 | 2.38e-42 | 143 |
| MsG0180005435.01.T01 | MTR_4g094535 | 97.059 | 68 | 2 | 0 | 1 | 68 | 363 | 430 | 5.44e-42 | 142 |
| MsG0180005435.01.T01 | MTR_3g113970 | 95.588 | 68 | 3 | 0 | 1 | 68 | 365 | 432 | 2.89e-41 | 140 |
| MsG0180005435.01.T01 | MTR_8g098360 | 89.706 | 68 | 7 | 0 | 1 | 68 | 363 | 430 | 1.75e-38 | 132 |
| MsG0180005435.01.T01 | MTR_3g110720 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.17e-13 | 64.7 |
| MsG0180005435.01.T01 | MTR_4g097830 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.22e-13 | 64.7 |
| MsG0180005435.01.T01 | MTR_1g106005 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.44e-13 | 64.7 |
| MsG0180005435.01.T01 | MTR_6g029190 | 39.189 | 74 | 45 | 0 | 2 | 75 | 375 | 448 | 1.84e-13 | 64.3 |
| MsG0180005435.01.T01 | MTR_4g093670 | 82.759 | 29 | 5 | 0 | 15 | 43 | 1 | 29 | 8.36e-13 | 58.2 |
| MsG0180005435.01.T01 | MTR_8g085980 | 40.984 | 61 | 36 | 0 | 2 | 62 | 374 | 434 | 1.06e-12 | 62.0 |
| MsG0180005435.01.T01 | MTR_7g085800 | 40.984 | 61 | 36 | 0 | 2 | 62 | 374 | 434 | 1.08e-12 | 62.0 |
| MsG0180005435.01.T01 | MTR_2g041670 | 35.443 | 79 | 51 | 0 | 2 | 80 | 369 | 447 | 6.85e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005435.01.T02 | AT2G29550 | 100.000 | 55 | 0 | 0 | 1 | 55 | 377 | 431 | 1.89e-33 | 119 |
| MsG0180005435.01.T02 | AT1G75780 | 100.000 | 54 | 0 | 0 | 1 | 54 | 378 | 431 | 1.05e-32 | 117 |
| MsG0180005435.01.T02 | AT5G62700 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.12e-32 | 117 |
| MsG0180005435.01.T02 | AT5G62690 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.12e-32 | 117 |
| MsG0180005435.01.T02 | AT5G23860 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.15e-32 | 116 |
| MsG0180005435.01.T02 | AT5G23860 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.15e-32 | 116 |
| MsG0180005435.01.T02 | AT5G12250 | 100.000 | 54 | 0 | 0 | 1 | 54 | 377 | 430 | 1.16e-32 | 116 |
| MsG0180005435.01.T02 | AT1G20010 | 98.148 | 54 | 1 | 0 | 1 | 54 | 378 | 431 | 2.90e-32 | 115 |
| MsG0180005435.01.T02 | AT5G44340 | 98.148 | 54 | 1 | 0 | 1 | 54 | 377 | 430 | 2.96e-32 | 115 |
| MsG0180005435.01.T02 | AT4G20890 | 96.296 | 54 | 2 | 0 | 1 | 54 | 377 | 430 | 1.04e-31 | 114 |
| MsG0180005435.01.T03 | AT2G29550 | 100.000 | 44 | 0 | 0 | 1 | 44 | 388 | 431 | 1.83e-25 | 96.7 |
| MsG0180005435.01.T03 | AT1G75780 | 100.000 | 43 | 0 | 0 | 1 | 43 | 389 | 431 | 9.63e-25 | 94.7 |
| MsG0180005435.01.T03 | AT5G62700 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.02e-24 | 94.7 |
| MsG0180005435.01.T03 | AT5G62690 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.02e-24 | 94.7 |
| MsG0180005435.01.T03 | AT5G23860 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.04e-24 | 94.7 |
| MsG0180005435.01.T03 | AT5G23860 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.04e-24 | 94.7 |
| MsG0180005435.01.T03 | AT5G12250 | 100.000 | 43 | 0 | 0 | 1 | 43 | 388 | 430 | 1.10e-24 | 94.7 |
| MsG0180005435.01.T03 | AT1G20010 | 97.674 | 43 | 1 | 0 | 1 | 43 | 389 | 431 | 2.68e-24 | 93.6 |
| MsG0180005435.01.T03 | AT5G44340 | 97.674 | 43 | 1 | 0 | 1 | 43 | 388 | 430 | 2.72e-24 | 93.6 |
| MsG0180005435.01.T03 | AT4G20890 | 95.349 | 43 | 2 | 0 | 1 | 43 | 388 | 430 | 8.90e-24 | 92.0 |
| MsG0180005435.01.T04 | AT2G29550 | 100.000 | 29 | 0 | 0 | 1 | 29 | 403 | 431 | 6.32e-13 | 60.8 |
| MsG0180005435.01.T04 | AT5G62700 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 3.11e-12 | 58.9 |
| MsG0180005435.01.T04 | AT5G62690 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 3.11e-12 | 58.9 |
| MsG0180005435.01.T04 | AT5G12250 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 3.17e-12 | 58.9 |
| MsG0180005435.01.T04 | AT5G23860 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 3.17e-12 | 58.9 |
| MsG0180005435.01.T04 | AT5G23860 | 100.000 | 28 | 0 | 0 | 1 | 28 | 403 | 430 | 3.17e-12 | 58.9 |
| MsG0180005435.01.T04 | AT1G75780 | 100.000 | 28 | 0 | 0 | 1 | 28 | 404 | 431 | 3.27e-12 | 58.9 |
| MsG0180005435.01.T04 | AT1G20010 | 96.429 | 28 | 1 | 0 | 1 | 28 | 404 | 431 | 8.35e-12 | 57.8 |
| MsG0180005435.01.T04 | AT5G44340 | 96.429 | 28 | 1 | 0 | 1 | 28 | 403 | 430 | 8.51e-12 | 57.4 |
| MsG0180005435.01.T04 | AT4G20890 | 92.857 | 28 | 2 | 0 | 1 | 28 | 403 | 430 | 3.04e-11 | 55.8 |
| MsG0180005435.01.T01 | AT2G29550 | 100.000 | 69 | 0 | 0 | 1 | 69 | 363 | 431 | 1.13e-43 | 146 |
| MsG0180005435.01.T01 | AT5G23860 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 7.77e-43 | 144 |
| MsG0180005435.01.T01 | AT5G23860 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 7.77e-43 | 144 |
| MsG0180005435.01.T01 | AT5G62700 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 8.06e-43 | 144 |
| MsG0180005435.01.T01 | AT5G62690 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 8.06e-43 | 144 |
| MsG0180005435.01.T01 | AT5G12250 | 100.000 | 68 | 0 | 0 | 1 | 68 | 363 | 430 | 9.00e-43 | 144 |
| MsG0180005435.01.T01 | AT1G75780 | 98.529 | 68 | 1 | 0 | 1 | 68 | 364 | 431 | 1.25e-42 | 144 |
| MsG0180005435.01.T01 | AT5G44340 | 98.529 | 68 | 1 | 0 | 1 | 68 | 363 | 430 | 1.97e-42 | 143 |
| MsG0180005435.01.T01 | AT4G20890 | 97.059 | 68 | 2 | 0 | 1 | 68 | 363 | 430 | 7.26e-42 | 142 |
| MsG0180005435.01.T01 | AT1G20010 | 95.588 | 68 | 3 | 0 | 1 | 68 | 364 | 431 | 1.10e-41 | 141 |
| MsG0180005435.01.T01 | AT4G14960 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.42e-13 | 64.7 |
| MsG0180005435.01.T01 | AT1G04820 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.45e-13 | 64.7 |
| MsG0180005435.01.T01 | AT1G50010 | 44.262 | 61 | 34 | 0 | 2 | 62 | 374 | 434 | 1.45e-13 | 64.7 |
| MsG0180005435.01.T01 | AT5G19780 | 40.984 | 61 | 36 | 0 | 2 | 62 | 374 | 434 | 1.12e-12 | 62.0 |
| MsG0180005435.01.T01 | AT5G19770 | 40.984 | 61 | 36 | 0 | 2 | 62 | 374 | 434 | 1.12e-12 | 62.0 |
| MsG0180005435.01.T01 | AT1G64740 | 40.984 | 61 | 36 | 0 | 2 | 62 | 374 | 434 | 1.13e-12 | 62.0 |
Find 22 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTCTTGCATTGGTATAC+TGG | 0.248415 | 1:-91841280 | MsG0180005435.01.T01:CDS |
| TGGTAACTCCACATCAATTC+AGG | 0.298154 | 1:-91841353 | MsG0180005435.01.T01:CDS |
| AGATGAGGAGGAAGAAGTTC+AGG | 0.309205 | 1:-91841143 | MsG0180005435.01.T01:CDS |
| CATCAATTCAGGAGATGTTT+AGG | 0.317382 | 1:-91841342 | MsG0180005435.01.T01:CDS |
| CAGTAGCATCTTGGTATTGC+TGG | 0.336251 | 1:+91841191 | None:intergenic |
| GTAGAAAAGCTTTCTTGCAT+TGG | 0.442451 | 1:-91841288 | MsG0180005435.01.T01:CDS |
| CTTCATCTGCAGTAGCATCT+TGG | 0.477927 | 1:+91841182 | None:intergenic |
| TGGAGAGGGTATGGATGAGA+TGG | 0.489491 | 1:-91841260 | MsG0180005435.01.T01:CDS |
| TTGGTATACTGGAGAGGGTA+TGG | 0.494465 | 1:-91841269 | MsG0180005435.01.T01:CDS |
| TGAGGAGGAAGAAGTTCAGG+AGG | 0.525475 | 1:-91841140 | MsG0180005435.01.T01:CDS |
| TTGCATTGGTATACTGGAGA+GGG | 0.526307 | 1:-91841274 | MsG0180005435.01.T01:CDS |
| GGGGTATGACTATGAAGATG+AGG | 0.526329 | 1:-91841158 | MsG0180005435.01.T01:CDS |
| GAGGGTGAGTGAGCAATTCA+CGG | 0.553083 | 1:-91841320 | MsG0180005435.01.T01:CDS |
| CAATTCAGGAGATGTTTAGG+AGG | 0.560407 | 1:-91841339 | MsG0180005435.01.T01:CDS |
| AGATGCTACTGCAGATGAAG+AGG | 0.562679 | 1:-91841179 | MsG0180005435.01.T01:CDS |
| CTTGCATTGGTATACTGGAG+AGG | 0.565550 | 1:-91841275 | MsG0180005435.01.T01:CDS |
| GTATGACTATGAAGATGAGG+AGG | 0.571612 | 1:-91841155 | MsG0180005435.01.T01:CDS |
| AAACATCTCCTGAATTGATG+TGG | 0.594428 | 1:+91841345 | None:intergenic |
| GATGCTACTGCAGATGAAGA+GGG | 0.594540 | 1:-91841178 | MsG0180005435.01.T01:CDS |
| AATTCAGGAGATGTTTAGGA+GGG | 0.623258 | 1:-91841338 | MsG0180005435.01.T01:CDS |
| ATGCTACTGCAGATGAAGAG+GGG | 0.676889 | 1:-91841177 | MsG0180005435.01.T01:CDS |
| ATTGCTGGTACTCTGAGACA+AGG | 0.726640 | 1:+91841206 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGCAAGAAAGCTTTTCTA+CGG | + | Chr1:91841212-91841231 | None:intergenic | 30.0% |
| AAACATCTCCTGAATTGATG+TGG | + | Chr1:91841157-91841176 | None:intergenic | 35.0% | |
| AATTCAGGAGATGTTTAGGA+GGG | - | Chr1:91841161-91841180 | MsG0180005435.01.T01:CDS | 35.0% | |
| CATCAATTCAGGAGATGTTT+AGG | - | Chr1:91841157-91841176 | MsG0180005435.01.T01:CDS | 35.0% | |
| GTAGAAAAGCTTTCTTGCAT+TGG | - | Chr1:91841211-91841230 | MsG0180005435.01.T01:CDS | 35.0% | |
| CAATTCAGGAGATGTTTAGG+AGG | - | Chr1:91841160-91841179 | MsG0180005435.01.T01:CDS | 40.0% | |
| GTATGACTATGAAGATGAGG+AGG | - | Chr1:91841344-91841363 | MsG0180005435.01.T01:CDS | 40.0% | |
| TGGTAACTCCACATCAATTC+AGG | - | Chr1:91841146-91841165 | MsG0180005435.01.T01:CDS | 40.0% | |
| ! | GAAAGCTTTTCTACGGAACA+TGG | + | Chr1:91841205-91841224 | None:intergenic | 40.0% |
| ! | GCTTTCTTGCATTGGTATAC+TGG | - | Chr1:91841219-91841238 | MsG0180005435.01.T01:CDS | 40.0% |
| ! | TTGCATTGGTATACTGGAGA+GGG | - | Chr1:91841225-91841244 | MsG0180005435.01.T01:CDS | 40.0% |
| AGATGAGGAGGAAGAAGTTC+AGG | - | Chr1:91841356-91841375 | MsG0180005435.01.T01:CDS | 45.0% | |
| AGATGCTACTGCAGATGAAG+AGG | - | Chr1:91841320-91841339 | MsG0180005435.01.T01:CDS | 45.0% | |
| ATGCTACTGCAGATGAAGAG+GGG | - | Chr1:91841322-91841341 | MsG0180005435.01.T01:CDS | 45.0% | |
| CAGTAGCATCTTGGTATTGC+TGG | + | Chr1:91841311-91841330 | None:intergenic | 45.0% | |
| CTTCATCTGCAGTAGCATCT+TGG | + | Chr1:91841320-91841339 | None:intergenic | 45.0% | |
| GATGCTACTGCAGATGAAGA+GGG | - | Chr1:91841321-91841340 | MsG0180005435.01.T01:CDS | 45.0% | |
| GGGGTATGACTATGAAGATG+AGG | - | Chr1:91841341-91841360 | MsG0180005435.01.T01:CDS | 45.0% | |
| TTGGTATACTGGAGAGGGTA+TGG | - | Chr1:91841230-91841249 | MsG0180005435.01.T01:CDS | 45.0% | |
| ! | CTTGCATTGGTATACTGGAG+AGG | - | Chr1:91841224-91841243 | MsG0180005435.01.T01:CDS | 45.0% |
| !! | ATTGCTGGTACTCTGAGACA+AGG | + | Chr1:91841296-91841315 | None:intergenic | 45.0% |
| GAGGGTGAGTGAGCAATTCA+CGG | - | Chr1:91841179-91841198 | MsG0180005435.01.T01:CDS | 50.0% | |
| TGAGGAGGAAGAAGTTCAGG+AGG | - | Chr1:91841359-91841378 | MsG0180005435.01.T01:CDS | 50.0% | |
| ! | TGGAGAGGGTATGGATGAGA+TGG | - | Chr1:91841239-91841258 | MsG0180005435.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 91841129 | 91841392 | 91841129 | ID=MsG0180005435.01;Name=MsG0180005435.01 |
| Chr1 | mRNA | 91841129 | 91841392 | 91841129 | ID=MsG0180005435.01.T01;Parent=MsG0180005435.01;Name=MsG0180005435.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|87 |
| Chr1 | exon | 91841129 | 91841392 | 91841129 | ID=MsG0180005435.01.T01:exon:10958;Parent=MsG0180005435.01.T01 |
| Chr1 | CDS | 91841129 | 91841392 | 91841129 | ID=MsG0180005435.01.T01:cds;Parent=MsG0180005435.01.T01 |
| Chr1 | mRNA | 91841129 | 91841350 | 91841129 | ID=MsG0180005435.01.T02;Parent=MsG0180005435.01;Name=MsG0180005435.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|73 |
| Chr1 | exon | 91841129 | 91841350 | 91841129 | ID=MsG0180005435.01.T02:exon:10959;Parent=MsG0180005435.01.T02 |
| Chr1 | CDS | 91841129 | 91841350 | 91841129 | ID=MsG0180005435.01.T02:cds;Parent=MsG0180005435.01.T02 |
| Chr1 | mRNA | 91841129 | 91841317 | 91841129 | ID=MsG0180005435.01.T03;Parent=MsG0180005435.01;Name=MsG0180005435.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|62 |
| Chr1 | exon | 91841129 | 91841317 | 91841129 | ID=MsG0180005435.01.T03:exon:10960;Parent=MsG0180005435.01.T03 |
| Chr1 | CDS | 91841129 | 91841317 | 91841129 | ID=MsG0180005435.01.T03:cds;Parent=MsG0180005435.01.T03 |
| Chr1 | mRNA | 91841129 | 91841272 | 91841129 | ID=MsG0180005435.01.T04;Parent=MsG0180005435.01;Name=MsG0180005435.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|47 |
| Chr1 | exon | 91841129 | 91841272 | 91841129 | ID=MsG0180005435.01.T04:exon:10961;Parent=MsG0180005435.01.T04 |
| Chr1 | CDS | 91841129 | 91841272 | 91841129 | ID=MsG0180005435.01.T04:cds;Parent=MsG0180005435.01.T04 |
| Gene Sequence |
| Protein sequence |
