AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005912.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005912.01.T01 MTR_1g109350 94.477 344 19 0 1 344 1 344 0.0 673
MsG0180005912.01.T01 MTR_5g069480 47.541 122 60 2 1 119 1 121 2.32e-31 120
MsG0180005912.01.T01 MTR_5g069480 47.541 122 60 2 1 119 1 121 3.91e-31 122
MsG0180005912.01.T01 MTR_3g082630 44.860 107 57 1 1 105 1 107 5.16e-25 105
MsG0180005912.01.T01 MTR_2g088900 46.154 104 55 1 1 103 97 200 4.12e-23 100
MsG0180005912.01.T01 MTR_7g108150 38.679 106 63 1 3 106 18 123 2.17e-20 91.3
MsG0180005912.01.T01 MTR_7g083540 33.333 117 69 1 1 108 1 117 3.11e-17 82.4
MsG0180005912.01.T01 MTR_1g013450 34.146 82 54 0 3 84 4 85 4.19e-13 69.7
MsG0180005912.01.T01 MTR_3g105710 34.783 92 57 1 3 91 4 95 5.21e-12 65.9
MsG0180005912.01.T01 MTR_7g018170 37.838 74 46 0 3 76 24 97 2.93e-11 64.7
MsG0180005912.01.T01 MTR_4g128930 34.146 82 54 0 3 84 20 101 5.46e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005912.01.T01 AT1G68190 38.535 314 135 8 1 311 10 268 4.35e-65 210
MsG0180005912.01.T01 AT1G68190 38.535 314 135 8 1 311 10 268 4.35e-65 210
MsG0180005912.01.T01 AT1G68190 38.535 314 135 8 1 311 39 297 1.27e-64 209
MsG0180005912.01.T01 AT5G48250 45.455 110 55 1 1 105 1 110 1.36e-27 111
MsG0180005912.01.T01 AT3G07650 41.880 117 56 2 1 105 1 117 9.48e-25 103
MsG0180005912.01.T01 AT3G07650 41.880 117 56 2 1 105 1 117 9.48e-25 103
MsG0180005912.01.T01 AT3G07650 41.880 117 56 2 1 105 1 117 9.48e-25 103
MsG0180005912.01.T01 AT3G07650 41.880 117 56 2 1 105 1 117 9.48e-25 103
MsG0180005912.01.T01 AT2G33500 39.252 107 62 1 5 108 12 118 1.52e-22 97.8
MsG0180005912.01.T01 AT2G33500 39.252 107 62 1 5 108 12 118 1.55e-22 97.8
MsG0180005912.01.T01 AT1G28050 38.889 108 63 1 5 109 9 116 6.43e-22 96.3
MsG0180005912.01.T01 AT3G21880 42.308 104 59 1 1 103 1 104 2.18e-21 94.4
MsG0180005912.01.T01 AT3G21880 42.308 104 59 1 1 103 5 108 2.19e-21 94.4
MsG0180005912.01.T01 AT3G21880 42.308 104 59 1 1 103 5 108 2.19e-21 94.4
MsG0180005912.01.T01 AT2G47890 38.679 106 64 1 2 106 10 115 1.99e-20 90.5
MsG0180005912.01.T01 AT2G47890 38.679 106 64 1 2 106 10 115 2.71e-20 90.5
MsG0180005912.01.T01 AT2G47890 38.679 106 64 1 2 106 10 115 3.51e-20 88.6
MsG0180005912.01.T01 AT4G15250 37.500 104 64 1 1 103 1 104 1.34e-19 88.6
MsG0180005912.01.T01 AT5G24930 40.244 82 49 0 3 84 4 85 1.69e-14 74.3
MsG0180005912.01.T01 AT3G02380 39.024 82 50 0 3 84 14 95 2.18e-13 70.9
MsG0180005912.01.T01 AT5G15840 37.000 100 62 1 3 102 18 116 4.68e-13 70.1
MsG0180005912.01.T01 AT5G15850 41.463 82 48 0 3 84 10 91 6.49e-13 69.3
MsG0180005912.01.T01 AT5G15840 37.000 100 62 1 3 102 18 116 7.34e-13 68.6
MsG0180005912.01.T01 AT2G24790 35.366 82 53 0 3 84 6 87 1.18e-12 68.2
MsG0180005912.01.T01 AT2G24790 35.366 82 53 0 3 84 6 87 1.88e-12 66.2

Find 84 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCATGGAGAGTTATTT+TGG 0.172335 1:-97465988 MsG0180005912.01.T01:CDS
CCACCAAACATCACTCTAAT+TGG 0.186429 1:+97465177 None:intergenic
ACTATGGGGATTTGAATTTA+AGG 0.203446 1:-97466981 MsG0180005912.01.T01:CDS
CAAAACTATGAGGAACTATT+TGG 0.211793 1:-97465213 MsG0180005912.01.T01:CDS
AGCAGATTGTTGATTTAAAA+AGG 0.229527 1:-97466219 MsG0180005912.01.T01:CDS
CAAATAGTTCCTCATAGTTT+TGG 0.233633 1:+97465214 None:intergenic
GATCATTCTACAAAGATAAC+TGG 0.292316 1:-97466175 MsG0180005912.01.T01:CDS
TTCTTTACTTTCTCTTGGAT+TGG 0.300222 1:-97468199 MsG0180005912.01.T01:five_prime_UTR
CCATAGTGTTGCAAACTCTT+TGG 0.316623 1:+97466997 None:intergenic
CGATCTGTCAAATCTTTCAT+TGG 0.318044 1:-97467031 MsG0180005912.01.T01:CDS
CCAAAGAGTTTGCAACACTA+TGG 0.332185 1:-97466997 MsG0180005912.01.T01:CDS
AAATTACATTTGAATGCTTT+CGG 0.333038 1:+97464744 None:intergenic
ATTTCCATGACCAACAATAA+TGG 0.336940 1:+97464773 None:intergenic
ATCACTTCTTTACTTTCTCT+TGG 0.349382 1:-97468204 MsG0180005912.01.T01:five_prime_UTR
AGACGTCTCTTATTCTTCAT+TGG 0.353797 1:-97465151 MsG0180005912.01.T01:CDS
ATGTTTATCTTGTTTGTAAA+GGG 0.368146 1:+97468225 None:intergenic
TTCTTGTTGTCCATTATTGT+TGG 0.381018 1:-97464783 MsG0180005912.01.T01:three_prime_UTR
AATTGTCCGGCCGGCACCTC+CGG 0.395024 1:-97467174 MsG0180005912.01.T01:CDS
AATTAGACCTCCATTGTACT+TGG 0.396036 1:+97465090 None:intergenic
TTTGTCTTCATCTTCAGTTA+TGG 0.410820 1:-97466017 MsG0180005912.01.T01:CDS
TTGTAGATGCCATTTGCATC+AGG 0.416030 1:+97467362 None:intergenic
AAGTCTACAACTAAAGGAAA+TGG 0.417116 1:-97467302 MsG0180005912.01.T01:exon
AAATGTAATTTGAATTAGAA+AGG 0.419682 1:-97464732 MsG0180005912.01.T01:three_prime_UTR
CCAATGGACCTTTGCATCAC+AGG 0.442091 1:+97467204 None:intergenic
TGGAATGTTAAGTCAACGTC+AGG 0.452059 1:+97465234 None:intergenic
GATCCAATTAGAGTGATGTT+TGG 0.455146 1:-97465180 MsG0180005912.01.T01:CDS
GTCTGGTGACAATCATGATA+AGG 0.459630 1:-97466849 MsG0180005912.01.T01:CDS
TCAGTCACTTCAACGACGTC+TGG 0.463294 1:-97466866 MsG0180005912.01.T01:CDS
CTCTTATTCTTCATTGGAGA+AGG 0.464404 1:-97465145 MsG0180005912.01.T01:CDS
GAAGAATAAACATTATGTTC+AGG 0.471002 1:-97464924 MsG0180005912.01.T01:three_prime_UTR
GATGTTTATCTTGTTTGTAA+AGG 0.472684 1:+97468224 None:intergenic
TGTTTGAAGTCTACAACTAA+AGG 0.472949 1:-97467308 MsG0180005912.01.T01:five_prime_UTR
TTGTCCATTATTGTTGGTCA+TGG 0.480987 1:-97464777 MsG0180005912.01.T01:three_prime_UTR
CATTGGGCAAATGAATTGTC+CGG 0.481757 1:-97467187 MsG0180005912.01.T01:CDS
TACCTGACCTAGTTGACTGC+TGG 0.483505 1:+97466823 None:intergenic
ACACAAGGGTCCGGAGGTGC+CGG 0.485126 1:+97467164 None:intergenic
CAAAGAGTTTGCAACACTAT+GGG 0.487640 1:-97466996 MsG0180005912.01.T01:CDS
TCTGGTGACAATCATGATAA+GGG 0.492151 1:-97466848 MsG0180005912.01.T01:CDS
CTGTGATGCAAAGGTCCATT+GGG 0.492787 1:-97467203 MsG0180005912.01.T01:CDS
CTTAACATTCCAAAACTATG+AGG 0.496040 1:-97465223 MsG0180005912.01.T01:CDS
ATCCAGCAGTCAACTAGGTC+AGG 0.498273 1:-97466825 MsG0180005912.01.T01:intron
GAGATTTCTCCTGATGCAAA+TGG 0.501748 1:-97467371 MsG0180005912.01.T01:five_prime_UTR
GTTATGGACCTTCCCTTGCA+TGG 0.501977 1:-97466001 MsG0180005912.01.T01:CDS
CTTACAACTTCTATCAAAAG+AGG 0.502880 1:-97464859 MsG0180005912.01.T01:three_prime_UTR
TGATAATTAAAGTGCTTGAT+CGG 0.503663 1:-97465039 MsG0180005912.01.T01:three_prime_UTR
CCTGTGATGCAAAGGTCCAT+TGG 0.506685 1:-97467204 MsG0180005912.01.T01:CDS
TGGGAGAAGACAATTCTATC+GGG 0.510510 1:-97466087 MsG0180005912.01.T01:CDS
CTGGGAGAAGACAATTCTAT+CGG 0.513285 1:-97466088 MsG0180005912.01.T01:CDS
TGCGATCAAAAGCTACATGA+TGG 0.526346 1:-97467073 MsG0180005912.01.T01:CDS
CCAAAATAACTCTCCATGCA+AGG 0.527678 1:+97465988 None:intergenic
CCAATTAGAGTGATGTTTGG+TGG 0.535280 1:-97465177 MsG0180005912.01.T01:CDS
TCTTGCGTATGTTCAGTGCT+TGG 0.538169 1:-97467122 MsG0180005912.01.T01:CDS
ACGAGTTACACACAAGGGTC+CGG 0.543558 1:+97467155 None:intergenic
CAAGTTCTTCACATATTCCC+AGG 0.546412 1:+97465280 None:intergenic
TGAAAACAGTGTCGATCAGA+AGG 0.550060 1:-97466954 MsG0180005912.01.T01:CDS
GTTTCAGCTAATAGAAGAGA+GGG 0.550624 1:-97466197 MsG0180005912.01.T01:CDS
AACTATGAGGAACTATTTGG+AGG 0.557150 1:-97465210 MsG0180005912.01.T01:CDS
CGGACAATTCATTTGCCCAA+TGG 0.558043 1:+97467188 None:intergenic
GTTGCAAACTCTTTGGCAGA+AGG 0.563765 1:+97467004 None:intergenic
AAGGGATCCAGCAGTCAACT+AGG 0.570420 1:-97466830 MsG0180005912.01.T01:CDS
GCAGCAAAATATTCAAGACC+TGG 0.572888 1:-97465298 MsG0180005912.01.T01:CDS
TTGTCTATCCTGTGATGCAA+AGG 0.577128 1:-97467212 MsG0180005912.01.T01:CDS
CAAAATAACTCTCCATGCAA+GGG 0.584415 1:+97465989 None:intergenic
AAGGGTCCGGAGGTGCCGGC+CGG 0.586330 1:+97467168 None:intergenic
AAAGATAACTGGGCTACAAG+TGG 0.592394 1:-97466164 MsG0180005912.01.T01:CDS
GGGCAAATGAATTGTCCGGC+CGG 0.594066 1:-97467183 MsG0180005912.01.T01:CDS
CTGATTCTTATGATCGAAAG+CGG 0.599433 1:-97464995 MsG0180005912.01.T01:three_prime_UTR
ATAACTCTCCATGCAAGGGA+AGG 0.600276 1:+97465993 None:intergenic
CAGCAAAATATTCAAGACCT+GGG 0.612414 1:-97465297 MsG0180005912.01.T01:CDS
ATCATTCTACAAAGATAACT+GGG 0.615835 1:-97466174 MsG0180005912.01.T01:CDS
GCGATCAAAAGCTACATGAT+GGG 0.617031 1:-97467072 MsG0180005912.01.T01:CDS
GGTTTCAGCTAATAGAAGAG+AGG 0.617889 1:-97466198 MsG0180005912.01.T01:CDS
AAAGAGTTTGCAACACTATG+GGG 0.619686 1:-97466995 MsG0180005912.01.T01:CDS
TTAATTATCAATTCACTGAG+AGG 0.633365 1:+97465052 None:intergenic
TATTGACAATCCAAGTACAA+TGG 0.635806 1:-97465100 MsG0180005912.01.T01:CDS
TCAGCATTACAGTAAACAAG+TGG 0.650082 1:+97467247 None:intergenic
TTGACAGATCGTTTGCGATG+TGG 0.663808 1:+97467043 None:intergenic
TGACAATCCAAGTACAATGG+AGG 0.672160 1:-97465097 MsG0180005912.01.T01:CDS
AGTTACACACAAGGGTCCGG+AGG 0.682408 1:+97467158 None:intergenic
AAGTTCACTACAAAGTAACC+AGG 0.683289 1:-97465957 MsG0180005912.01.T01:intron
GGGAGAAGACAATTCTATCG+GGG 0.687222 1:-97466086 MsG0180005912.01.T01:CDS
AACAGCACGAGTTACACACA+AGG 0.692236 1:+97467149 None:intergenic
TTGCATCACAGGATAGACAA+AGG 0.703130 1:+97467215 None:intergenic
ACAGCACGAGTTACACACAA+GGG 0.726480 1:+97467150 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTTTATTCTTGATT+AGG + Chr1:97467239-97467258 None:intergenic 10.0%
!! AAAATCTAAATATATTTCCA+AGG + Chr1:97467072-97467091 None:intergenic 15.0%
!! AAATCTAAATATATTTCCAA+GGG + Chr1:97467071-97467090 None:intergenic 15.0%
!! AAATGTAATTTGAATTAGAA+AGG - Chr1:97468203-97468222 MsG0180005912.01.T01:five_prime_UTR 15.0%
!! ATAAAAATAAATTCATACTC+TGG - Chr1:97467247-97467266 MsG0180005912.01.T01:CDS 15.0%
!! ATATTAAAATATACCCTATA+TGG - Chr1:97467152-97467171 MsG0180005912.01.T01:CDS 15.0%
!! TATTAAAATATACCCTATAT+GGG - Chr1:97467153-97467172 MsG0180005912.01.T01:CDS 15.0%
!!! TAAAAAGTTTTGTAAACAAT+GGG - Chr1:97465310-97465329 MsG0180005912.01.T01:intron 15.0%
!!! TTAAAAAGTTTTGTAAACAA+TGG - Chr1:97465309-97465328 MsG0180005912.01.T01:intron 15.0%
!!! TTGGAAATATATTTAGATTT+TGG - Chr1:97467071-97467090 MsG0180005912.01.T01:CDS 15.0%
!! AAATTACATTTGAATGCTTT+CGG + Chr1:97468194-97468213 None:intergenic 20.0%
!! ATAAGATATCACAGAAATTA+AGG - Chr1:97467276-97467295 MsG0180005912.01.T01:CDS 20.0%
!!! ATGTTTATCTTGTTTGTAAA+GGG + Chr1:97464713-97464732 None:intergenic 20.0%
!!! GATTTTTAGTATACAATCAT+AGG - Chr1:97464797-97464816 MsG0180005912.01.T01:three_prime_UTR 20.0%
!!! GTGTTTTTAAGATTATTAGT+TGG + Chr1:97467115-97467134 None:intergenic 20.0%
!!! TGTGGTTTTGATAATTAATA+TGG + Chr1:97466594-97466613 None:intergenic 20.0%
!!! TTATGTATGACTAATTTTGA+AGG - Chr1:97467970-97467989 MsG0180005912.01.T01:intron 20.0%
!!! TTGAATCACTTTTTCTTTTA+TGG - Chr1:97465380-97465399 MsG0180005912.01.T01:intron 20.0%
! AAAGGAAACATACATTATGT+GGG - Chr1:97467039-97467058 MsG0180005912.01.T01:CDS 25.0%
! AAATGCAGAAGAAAAAGTTT+CGG + Chr1:97466884-97466903 None:intergenic 25.0%
! AAGGGTTAAAGTATGTAATA+GGG + Chr1:97467344-97467363 None:intergenic 25.0%
! AAGTTTAAATAAATGTCTGC+TGG + Chr1:97467474-97467493 None:intergenic 25.0%
! ACATTATGTTCAGGAAAAAA+AGG - Chr1:97468020-97468039 MsG0180005912.01.T01:intron 25.0%
! ATCATTCTACAAAGATAACT+GGG - Chr1:97466761-97466780 MsG0180005912.01.T01:intron 25.0%
! ATCATTTGAATTTCCCATAT+AGG + Chr1:97467169-97467188 None:intergenic 25.0%
! ATTATCTTCATCCAAAATCT+TGG - Chr1:97466392-97466411 MsG0180005912.01.T01:intron 25.0%
! ATTTGAAAATCTACATCAGT+GGG + Chr1:97464910-97464929 None:intergenic 25.0%
! CTACTTTGTTGAAATTCAAA+TGG - Chr1:97464989-97465008 MsG0180005912.01.T01:three_prime_UTR 25.0%
! CTTCCAATTCTGATAATAAA+AGG - Chr1:97467190-97467209 MsG0180005912.01.T01:CDS 25.0%
! GAAGAATAAACATTATGTTC+AGG - Chr1:97468011-97468030 MsG0180005912.01.T01:intron 25.0%
! GAATGCATTAATCATTTCTT+CGG + Chr1:97466423-97466442 None:intergenic 25.0%
! GATTAATGCATTCTTAACTA+AGG - Chr1:97466430-97466449 MsG0180005912.01.T01:intron 25.0%
! GTACTTTAACTAACACTTTA+AGG + Chr1:97466497-97466516 None:intergenic 25.0%
! TATAGATTTCATCAGTGATA+AGG + Chr1:97466163-97466182 None:intergenic 25.0%
! TCATTTGAATTTCCCATATA+GGG + Chr1:97467168-97467187 None:intergenic 25.0%
! TTAATTATCAATTCACTGAG+AGG + Chr1:97467886-97467905 None:intergenic 25.0%
! TTATCTTCATCCAAAATCTT+GGG - Chr1:97466393-97466412 MsG0180005912.01.T01:intron 25.0%
! TTTACCTTCTCATAAATGAT+TGG - Chr1:97466224-97466243 MsG0180005912.01.T01:CDS 25.0%
!! ATTGTTTAGTATTGAACGTT+AGG - Chr1:97467554-97467573 MsG0180005912.01.T01:intron 25.0%
!! CAGATGATGATTTTCTATTA+TGG + Chr1:97467923-97467942 None:intergenic 25.0%
!! CTTTTATTATCAGAATTGGA+AGG + Chr1:97467192-97467211 None:intergenic 25.0%
!! GATGTTTATCTTGTTTGTAA+AGG + Chr1:97464714-97464733 None:intergenic 25.0%
!! GGTCTTAATAATTGTTGTTA+TGG - Chr1:97465427-97465446 MsG0180005912.01.T01:intron 25.0%
!! GTTCCTTTTATTATCAGAAT+TGG + Chr1:97467196-97467215 None:intergenic 25.0%
!! TCTTGAATGCAACAATTTTT+CGG + Chr1:97468240-97468259 None:intergenic 25.0%
!! TGATAATTAAAGTGCTTGAT+CGG - Chr1:97467896-97467915 MsG0180005912.01.T01:intron 25.0%
!! TGATGTAGATTTTCAAATCT+GGG - Chr1:97464912-97464931 MsG0180005912.01.T01:three_prime_UTR 25.0%
!! TGTAAATCATTTTCTAACTC+TGG + Chr1:97466312-97466331 None:intergenic 25.0%
!!! AGCAGATTGTTGATTTAAAA+AGG - Chr1:97466716-97466735 MsG0180005912.01.T01:intron 25.0%
!!! TGCAACTATATTTGTTGTAA+AGG - Chr1:97465463-97465482 MsG0180005912.01.T01:intron 25.0%
!!! TTTAGATTTTGGTGTAGAAT+AGG - Chr1:97467082-97467101 MsG0180005912.01.T01:CDS 25.0%
AAGGAAACATACATTATGTG+GGG - Chr1:97467040-97467059 MsG0180005912.01.T01:CDS 30.0%
AAGGAGTTCGTAACAATTAT+AGG - Chr1:97466449-97466468 MsG0180005912.01.T01:intron 30.0%
AAGTCTACAACTAAAGGAAA+TGG - Chr1:97465633-97465652 MsG0180005912.01.T01:intron 30.0%
AATGCAGAAGAAAAAGTTTC+GGG + Chr1:97466883-97466902 None:intergenic 30.0%
ACTATGGGGATTTGAATTTA+AGG - Chr1:97465954-97465973 MsG0180005912.01.T01:intron 30.0%
AGAGGGATTGATCAAAATAA+TGG + Chr1:97467318-97467337 None:intergenic 30.0%
AGGGTTAAAGTATGTAATAG+GGG + Chr1:97467343-97467362 None:intergenic 30.0%
AGTACCAATCATTTATGAGA+AGG + Chr1:97466231-97466250 None:intergenic 30.0%
AGTTCGTAACAATTATAGGA+AGG - Chr1:97466453-97466472 MsG0180005912.01.T01:intron 30.0%
ATCACTTCTTTACTTTCTCT+TGG - Chr1:97464731-97464750 MsG0180005912.01.T01:three_prime_UTR 30.0%
ATGGTTAGTATGATAGTATG+TGG - Chr1:97465214-97465233 MsG0180005912.01.T01:CDS 30.0%
ATTTCCATGACCAACAATAA+TGG + Chr1:97468165-97468184 None:intergenic 30.0%
CAAAACTATGAGGAACTATT+TGG - Chr1:97467722-97467741 MsG0180005912.01.T01:intron 30.0%
CAAGGGTTAAAGTATGTAAT+AGG + Chr1:97467345-97467364 None:intergenic 30.0%
CTTAACATTCCAAAACTATG+AGG - Chr1:97467712-97467731 MsG0180005912.01.T01:intron 30.0%
CTTACAACTTCTATCAAAAG+AGG - Chr1:97468076-97468095 MsG0180005912.01.T01:intron 30.0%
GAAAGGAAACATACATTATG+TGG - Chr1:97467038-97467057 MsG0180005912.01.T01:CDS 30.0%
GATAGTGTATTATTAGCACA+TGG + Chr1:97464966-97464985 None:intergenic 30.0%
GATCATTCTACAAAGATAAC+TGG - Chr1:97466760-97466779 MsG0180005912.01.T01:intron 30.0%
GATTTGAAAATCTACATCAG+TGG + Chr1:97464911-97464930 None:intergenic 30.0%
TATCAAAACCACAAGTACAA+TGG - Chr1:97466601-97466620 MsG0180005912.01.T01:intron 30.0%
TATTGACAATCCAAGTACAA+TGG - Chr1:97467835-97467854 MsG0180005912.01.T01:intron 30.0%
TCTCAGATAACTGTTCAAAA+CGG - Chr1:97465406-97465425 MsG0180005912.01.T01:intron 30.0%
TGGTGTCTAAATAATTTCGT+GGG + Chr1:97465017-97465036 None:intergenic 30.0%
TGTATGTTTCCTTTCAATTG+TGG + Chr1:97467033-97467052 None:intergenic 30.0%
TTCTTTACTTTCTCTTGGAT+TGG - Chr1:97464736-97464755 MsG0180005912.01.T01:three_prime_UTR 30.0%
TTTGTCTTCATCTTCAGTTA+TGG - Chr1:97466918-97466937 MsG0180005912.01.T01:CDS 30.0%
TTTGTTGATGTTTGCAGTTA+TGG - Chr1:97467615-97467634 MsG0180005912.01.T01:intron 30.0%
! CAAATAGTTCCTCATAGTTT+TGG + Chr1:97467724-97467743 None:intergenic 30.0%
! CATTGATGATATATGAGTGA+TGG - Chr1:97465195-97465214 MsG0180005912.01.T01:CDS 30.0%
! CTGATGTAGATTTTCAAATC+TGG - Chr1:97464911-97464930 MsG0180005912.01.T01:three_prime_UTR 30.0%
! GATTTTGGATGAAGATAATG+TGG + Chr1:97466391-97466410 None:intergenic 30.0%
! GTTTTACTTCTAGTCAAAAG+TGG - Chr1:97467583-97467602 MsG0180005912.01.T01:intron 30.0%
! TATTTTGCTACTAATCTGCT+GGG - Chr1:97466829-97466848 MsG0180005912.01.T01:CDS 30.0%
! TGTTTGAAGTCTACAACTAA+AGG - Chr1:97465627-97465646 MsG0180005912.01.T01:intron 30.0%
! TTCTTGTTGTCCATTATTGT+TGG - Chr1:97468152-97468171 MsG0180005912.01.T01:five_prime_UTR 30.0%
!! AAGTACGATGATTCTTGTTT+TGG - Chr1:97466511-97466530 MsG0180005912.01.T01:intron 30.0%
!! TCTATTAACTCCACTTTTCA+TGG - Chr1:97465512-97465531 MsG0180005912.01.T01:intron 30.0%
!!! GATTCTTGTTTTGGTTTCTA+AGG - Chr1:97466520-97466539 MsG0180005912.01.T01:intron 30.0%
AAAGAGTTTGCAACACTATG+GGG - Chr1:97465940-97465959 MsG0180005912.01.T01:intron 35.0%
AACTATGAGGAACTATTTGG+AGG - Chr1:97467725-97467744 MsG0180005912.01.T01:intron 35.0%
AAGTTCACTACAAAGTAACC+AGG - Chr1:97466978-97466997 MsG0180005912.01.T01:CDS 35.0%
AATTAGACCTCCATTGTACT+TGG + Chr1:97467848-97467867 None:intergenic 35.0%
AGACGTCTCTTATTCTTCAT+TGG - Chr1:97467784-97467803 MsG0180005912.01.T01:intron 35.0%
AGATTTCATCAGTGATAAGG+AGG + Chr1:97466160-97466179 None:intergenic 35.0%
ATCAGTGGGAATTTAGAAGT+AGG + Chr1:97464896-97464915 None:intergenic 35.0%
ATGCAGAAGAAAAAGTTTCG+GGG + Chr1:97466882-97466901 None:intergenic 35.0%
CAAAATAACTCTCCATGCAA+GGG + Chr1:97466949-97466968 None:intergenic 35.0%
CAAAGAGTTTGCAACACTAT+GGG - Chr1:97465939-97465958 MsG0180005912.01.T01:intron 35.0%
CAAGTCTCGCTAAATTTATG+TGG + Chr1:97465037-97465056 None:intergenic 35.0%
CAGCAAAATATTCAAGACCT+GGG - Chr1:97467638-97467657 MsG0180005912.01.T01:intron 35.0%
CGATCTGTCAAATCTTTCAT+TGG - Chr1:97465904-97465923 MsG0180005912.01.T01:intron 35.0%
CTCTTATTCTTCATTGGAGA+AGG - Chr1:97467790-97467809 MsG0180005912.01.T01:intron 35.0%
CTGATTCTTATGATCGAAAG+CGG - Chr1:97467940-97467959 MsG0180005912.01.T01:intron 35.0%
CTGTTTGATTAATGTGTGCA+AGG + Chr1:97467425-97467444 None:intergenic 35.0%
GAAATCATCTCGACAAACAA+GGG + Chr1:97467362-97467381 None:intergenic 35.0%
GACTATTGAAGTCAAATGTC+AGG + Chr1:97467391-97467410 None:intergenic 35.0%
GAGAAAAAAAGTGAGAGAGA+TGG + Chr1:97464769-97464788 None:intergenic 35.0%
GATCCAATTAGAGTGATGTT+TGG - Chr1:97467755-97467774 MsG0180005912.01.T01:intron 35.0%
GGGTTAAAGTATGTAATAGG+GGG + Chr1:97467342-97467361 None:intergenic 35.0%
GTATTATTAGCACATGGAAG+TGG + Chr1:97464960-97464979 None:intergenic 35.0%
GTGGTGTCTAAATAATTTCG+TGG + Chr1:97465018-97465037 None:intergenic 35.0%
GTTTCAGCTAATAGAAGAGA+GGG - Chr1:97466738-97466757 MsG0180005912.01.T01:intron 35.0%
TCAGCATTACAGTAAACAAG+TGG + Chr1:97465691-97465710 None:intergenic 35.0%
TCCTATAGCTCCAATTCAAT+GGG + Chr1:97465347-97465366 None:intergenic 35.0%
TCTGGTGACAATCATGATAA+GGG - Chr1:97466087-97466106 MsG0180005912.01.T01:CDS 35.0%
TGAAATCATCTCGACAAACA+AGG + Chr1:97467363-97467382 None:intergenic 35.0%
TGTCAGATTTCCCATTGAAT+TGG - Chr1:97465334-97465353 MsG0180005912.01.T01:intron 35.0%
TTACATGTACGACATTGTGT+CGG - Chr1:97465167-97465186 MsG0180005912.01.T01:CDS 35.0%
TTAGGATTACTTGACGCTTA+AGG + Chr1:97467221-97467240 None:intergenic 35.0%
TTCAAATCTGGGCATATGTA+TGG - Chr1:97464923-97464942 MsG0180005912.01.T01:three_prime_UTR 35.0%
TTCTCAACACACACTCAAAT+TGG + Chr1:97465072-97465091 None:intergenic 35.0%
TTGTCCATTATTGTTGGTCA+TGG - Chr1:97468158-97468177 MsG0180005912.01.T01:five_prime_UTR 35.0%
TTTGGATGAAGATAATGTGG+AGG + Chr1:97466388-97466407 None:intergenic 35.0%
! GACATTTGACTTCAATAGTC+TGG - Chr1:97467391-97467410 MsG0180005912.01.T01:five_prime_UTR 35.0%
! GTATTTTGCTACTAATCTGC+TGG - Chr1:97466828-97466847 MsG0180005912.01.T01:CDS 35.0%
! TTTTATGCTGATCACAGCAT+TGG - Chr1:97466333-97466352 MsG0180005912.01.T01:intron 35.0%
AAAGATAACTGGGCTACAAG+TGG - Chr1:97466771-97466790 MsG0180005912.01.T01:intron 40.0%
ATGAAGCGATGAATCTTGTG+AGG - Chr1:97466252-97466271 MsG0180005912.01.T01:CDS 40.0%
CAAAACCACAAGTACAATGG+AGG - Chr1:97466604-97466623 MsG0180005912.01.T01:intron 40.0%
CAAGTTCTTCACATATTCCC+AGG + Chr1:97467658-97467677 None:intergenic 40.0%
CATTGGGCAAATGAATTGTC+CGG - Chr1:97465748-97465767 MsG0180005912.01.T01:intron 40.0%
CCAAAATAACTCTCCATGCA+AGG + Chr1:97466950-97466969 None:intergenic 40.0%
CCAAAGAGTTTGCAACACTA+TGG - Chr1:97465938-97465957 MsG0180005912.01.T01:intron 40.0%
CCAATTAGAGTGATGTTTGG+TGG - Chr1:97467758-97467777 MsG0180005912.01.T01:intron 40.0%
CCACCAAACATCACTCTAAT+TGG + Chr1:97467761-97467780 None:intergenic 40.0%
CCATAGTGTTGCAAACTCTT+TGG + Chr1:97465941-97465960 None:intergenic 40.0%
CCTGTTTAGTCCATGAAAAG+TGG + Chr1:97465525-97465544 None:intergenic 40.0%
CTCCTATAGCTCCAATTCAA+TGG + Chr1:97465348-97465367 None:intergenic 40.0%
CTGGGAGAAGACAATTCTAT+CGG - Chr1:97466847-97466866 MsG0180005912.01.T01:CDS 40.0%
GAGATTTCTCCTGATGCAAA+TGG - Chr1:97465564-97465583 MsG0180005912.01.T01:intron 40.0%
GCAGCAAAATATTCAAGACC+TGG - Chr1:97467637-97467656 MsG0180005912.01.T01:intron 40.0%
GCGATCAAAAGCTACATGAT+GGG - Chr1:97465863-97465882 MsG0180005912.01.T01:intron 40.0%
GGATAGATAAGTATCTGACC+TGG + Chr1:97466999-97467018 None:intergenic 40.0%
GGTTTCAGCTAATAGAAGAG+AGG - Chr1:97466737-97466756 MsG0180005912.01.T01:intron 40.0%
GTCTGGTGACAATCATGATA+AGG - Chr1:97466086-97466105 MsG0180005912.01.T01:CDS 40.0%
TCCCATTGAATTGGAGCTAT+AGG - Chr1:97465343-97465362 MsG0180005912.01.T01:intron 40.0%
TGAATTGGAGCTATAGGAGT+TGG - Chr1:97465349-97465368 MsG0180005912.01.T01:intron 40.0%
TGACAATCCAAGTACAATGG+AGG - Chr1:97467838-97467857 MsG0180005912.01.T01:intron 40.0%
TGCGATCAAAAGCTACATGA+TGG - Chr1:97465862-97465881 MsG0180005912.01.T01:intron 40.0%
TGGAATGTTAAGTCAACGTC+AGG + Chr1:97467704-97467723 None:intergenic 40.0%
TGGGAGAAGACAATTCTATC+GGG - Chr1:97466848-97466867 MsG0180005912.01.T01:CDS 40.0%
TGTTGCATGCACTGCTTTAT+GGG - Chr1:97464855-97464874 MsG0180005912.01.T01:three_prime_UTR 40.0%
TGTTGTGAGCCACAATTGAA+AGG - Chr1:97467021-97467040 MsG0180005912.01.T01:CDS 40.0%
TTGCATCACAGGATAGACAA+AGG + Chr1:97465723-97465742 None:intergenic 40.0%
TTGTCTATCCTGTGATGCAA+AGG - Chr1:97465723-97465742 MsG0180005912.01.T01:intron 40.0%
TTGTTGCATGCACTGCTTTA+TGG - Chr1:97464854-97464873 MsG0180005912.01.T01:three_prime_UTR 40.0%
TTTATGGGAGTTAAGAGTCG+TGG - Chr1:97464870-97464889 MsG0180005912.01.T01:three_prime_UTR 40.0%
! AATGTGGAGGCTCACATTTT+AGG + Chr1:97466375-97466394 None:intergenic 40.0%
! CCTTGCATGGAGAGTTATTT+TGG - Chr1:97466947-97466966 MsG0180005912.01.T01:CDS 40.0%
! CTTCGGTTTTCCCAAGATTT+TGG + Chr1:97466406-97466425 None:intergenic 40.0%
! GTGAATTTTGCACAGCATTG+AGG - Chr1:97465665-97465684 MsG0180005912.01.T01:intron 40.0%
! TTGTAGATGCCATTTGCATC+AGG + Chr1:97465576-97465595 None:intergenic 40.0%
!! CCACTTTTCATGGACTAAAC+AGG - Chr1:97465522-97465541 MsG0180005912.01.T01:intron 40.0%
!! TGAAAACAGTGTCGATCAGA+AGG - Chr1:97465981-97466000 MsG0180005912.01.T01:CDS 40.0%
AACAGCACGAGTTACACACA+AGG + Chr1:97465789-97465808 None:intergenic 45.0%
AAGCGATGAATCTTGTGAGG+AGG - Chr1:97466255-97466274 MsG0180005912.01.T01:CDS 45.0%
AAGTATGTAATAGGGGGCAG+AGG + Chr1:97467336-97467355 None:intergenic 45.0%
ACAGCACGAGTTACACACAA+GGG + Chr1:97465788-97465807 None:intergenic 45.0%
AGTATGTAATAGGGGGCAGA+GGG + Chr1:97467335-97467354 None:intergenic 45.0%
ATAACTCTCCATGCAAGGGA+AGG + Chr1:97466945-97466964 None:intergenic 45.0%
ATTATGTGGGGTACATCCCT+TGG - Chr1:97467052-97467071 MsG0180005912.01.T01:CDS 45.0%
CGGACAATTCATTTGCCCAA+TGG + Chr1:97465750-97465769 None:intergenic 45.0%
CTGTGATGCAAAGGTCCATT+GGG - Chr1:97465732-97465751 MsG0180005912.01.T01:intron 45.0%
GGGAGAAGACAATTCTATCG+GGG - Chr1:97466849-97466868 MsG0180005912.01.T01:CDS 45.0%
GTTGCAAACTCTTTGGCAGA+AGG + Chr1:97465934-97465953 None:intergenic 45.0%
TCAATTGTGGCTCACAACAG+TGG + Chr1:97467020-97467039 None:intergenic 45.0%
TCAGACCTCCATTGTACTTG+TGG + Chr1:97466612-97466631 None:intergenic 45.0%
TCTTGCGTATGTTCAGTGCT+TGG - Chr1:97465813-97465832 MsG0180005912.01.T01:intron 45.0%
TTGACAGATCGTTTGCGATG+TGG + Chr1:97465895-97465914 None:intergenic 45.0%
!!! TTTAAAATAATATTTTAACA+TGG + Chr1:97468271-97468290 None:intergenic 5.0%
AAGGGATCCAGCAGTCAACT+AGG - Chr1:97466105-97466124 MsG0180005912.01.T01:CDS 50.0%
ACGAGTTACACACAAGGGTC+CGG + Chr1:97465783-97465802 None:intergenic 50.0%
ATCCAGCAGTCAACTAGGTC+AGG - Chr1:97466110-97466129 MsG0180005912.01.T01:CDS 50.0%
CCAATGGACCTTTGCATCAC+AGG + Chr1:97465734-97465753 None:intergenic 50.0%
CCTGTGATGCAAAGGTCCAT+TGG - Chr1:97465731-97465750 MsG0180005912.01.T01:intron 50.0%
GTTATGGACCTTCCCTTGCA+TGG - Chr1:97466934-97466953 MsG0180005912.01.T01:CDS 50.0%
TACCTGACCTAGTTGACTGC+TGG + Chr1:97466115-97466134 None:intergenic 50.0%
TCAGTCACTTCAACGACGTC+TGG - Chr1:97466069-97466088 MsG0180005912.01.T01:CDS 50.0%
! GTGGAGGCTCACATTTTAGG+AGG + Chr1:97466372-97466391 None:intergenic 50.0%
AGTTACACACAAGGGTCCGG+AGG + Chr1:97465780-97465799 None:intergenic 55.0%
GGGCAAATGAATTGTCCGGC+CGG - Chr1:97465752-97465771 MsG0180005912.01.T01:intron 55.0%
AATTGTCCGGCCGGCACCTC+CGG - Chr1:97465761-97465780 MsG0180005912.01.T01:intron 65.0%
! ACACAAGGGTCCGGAGGTGC+CGG + Chr1:97465774-97465793 None:intergenic 65.0%
!! AAGGGTCCGGAGGTGCCGGC+CGG + Chr1:97465770-97465789 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 97464650 97468307 97464650 ID=MsG0180005912.01;Name=MsG0180005912.01
Chr1 mRNA 97464650 97468307 97464650 ID=MsG0180005912.01.T01;Parent=MsG0180005912.01;Name=MsG0180005912.01.T01;_AED=0.49;_eAED=0.49;_QI=277|1|1|1|0.66|0.5|4|442|344
Chr1 exon 97464650 97465325 97464650 ID=MsG0180005912.01.T01:exon:19605;Parent=MsG0180005912.01.T01
Chr1 exon 97465958 97466278 97465958 ID=MsG0180005912.01.T01:exon:19604;Parent=MsG0180005912.01.T01
Chr1 exon 97466826 97467413 97466826 ID=MsG0180005912.01.T01:exon:19603;Parent=MsG0180005912.01.T01
Chr1 exon 97468139 97468307 97468139 ID=MsG0180005912.01.T01:exon:19602;Parent=MsG0180005912.01.T01
Chr1 five_prime_UTR 97468139 97468307 97468139 ID=MsG0180005912.01.T01:five_prime_utr;Parent=MsG0180005912.01.T01
Chr1 five_prime_UTR 97467306 97467413 97467306 ID=MsG0180005912.01.T01:five_prime_utr;Parent=MsG0180005912.01.T01
Chr1 CDS 97466826 97467305 97466826 ID=MsG0180005912.01.T01:cds;Parent=MsG0180005912.01.T01
Chr1 CDS 97465958 97466278 97465958 ID=MsG0180005912.01.T01:cds;Parent=MsG0180005912.01.T01
Chr1 CDS 97465092 97465325 97465092 ID=MsG0180005912.01.T01:cds;Parent=MsG0180005912.01.T01
Chr1 three_prime_UTR 97464650 97465091 97464650 ID=MsG0180005912.01.T01:three_prime_utr;Parent=MsG0180005912.01.T01
Gene Sequence

>MsG0180005912.01.T01

ATGGAGAAAATTTGTGAATTTTGCACAGCATTGAGGCCACTTGTTTACTGTAATGCTGATGCAGCATACCTTTGTCTATCCTGTGATGCAAAGGTCCATTGGGCAAATGAATTGTCCGGCCGGCACCTCCGGACCCTTGTGTGTAACTCGTGCTGTTGTGATCTTGCGTATGTTCAGTGCTTGGATCACAAAATATTAATTTGTAGAGACTGCGATCAAAAGCTACATGATGGGTCTTCACCACATCGCAAACGATCTGTCAAATCTTTCATTGGTTGTCCTTCTGCCAAAGAGTTTGCAACACTATGGGGATTTGAATTTAAGGAGATTGAAAACAGTGTCGATCAGAAGGATCATTTTGCATCTATTTCTTGTGTTGCTACAGATGTAAATGTGATCAAGAATCGTATTCGAACTTCAGTCACTTCAACGACGTCTGGTGACAATCATGATAAGGGATCCAGCAGTCAACTAGGTCAGATATTGTACAGTGATCAAGAGAAGCAAACTATTCTGCAGCAGATTGTTGATTTAAAAAGGTTTCAGCTAATAGAAGAGAGGGATCATTCTACAAAGATAACTGGGCTACAAGTGGATGAAAAATTTAATCAACAAGCACAAAAATCTCAGTATTTTGCTACTAATCTGCTGGGAGAAGACAATTCTATCGGGGAGCTGAACCCCGAAACTTTTTCTTCTGCATTTTCTCAACTTGATAATTTGTCTTCATCTTCAGTTATGGACCTTCCCTTGCATGGAGAGTTATTTTGGACTTCTAAAAGTTCACTACAAAGTAACCAGTTATGGCAGCAAAATATTCAAGACCTGGGAATATGTGAAGAACTTGTTTGTCGAGACGATTTCAACATACCTGACGTTGACTTAACATTCCAAAACTATGAGGAACTATTTGGAGGAGATCAAGATCCAATTAGAGTGATGTTTGGTGGCAAAGACGTCTCTTATTCTTCATTGGAGAAGGATTTATCAGTTGATAATTCAGATATTGACAATCCAAGTACAATGGAGGTCTAA
Protein sequence

>MsG0180005912.01.T01

MEKICEFCTALRPLVYCNADAAYLCLSCDAKVHWANELSGRHLRTLVCNSCCCDLAYVQCLDHKILICRDCDQKLHDGSSPHRKRSVKSFIGCPSAKEFATLWGFEFKEIENSVDQKDHFASISCVATDVNVIKNRIRTSVTSTTSGDNHDKGSSSQLGQILYSDQEKQTILQQIVDLKRFQLIEERDHSTKITGLQVDEKFNQQAQKSQYFATNLLGEDNSIGELNPETFSSAFSQLDNLSSSSVMDLPLHGELFWTSKSSLQSNQLWQQNIQDLGICEELVCRDDFNIPDVDLTFQNYEELFGGDQDPIRVMFGGKDVSYSSLEKDLSVDNSDIDNPSTMEV*