AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016957.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016957.01.T01 MTR_3g099180 97.386 153 2 2 1 152 65 216 4.24e-105 300
MsG0380016957.01.T01 MTR_3g099180 97.386 153 2 2 1 152 65 216 4.63e-105 299
MsG0380016957.01.T01 MTR_7g113680 63.077 130 36 4 1 120 162 289 2.64e-48 158
MsG0380016957.01.T01 MTR_1g082660 65.000 120 30 4 1 109 111 229 4.16e-46 151
MsG0380016957.01.T01 MTR_2g023340 49.265 136 48 3 1 124 95 221 1.15e-34 121
MsG0380016957.01.T01 MTR_2g081600 55.263 76 34 0 2 77 34 109 3.21e-24 91.3
MsG0380016957.01.T01 MTR_2g081630 54.545 77 35 0 2 78 34 110 8.96e-24 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016957.01.T01 AT3G48590 85.816 141 13 3 1 135 75 214 3.13e-80 237
MsG0380016957.01.T01 AT5G63470 80.667 150 13 4 1 141 88 230 2.09e-77 231
MsG0380016957.01.T01 AT5G63470 80.667 150 13 4 1 141 88 230 2.09e-77 231
MsG0380016957.01.T01 AT1G54830 63.359 131 38 5 1 125 80 206 2.55e-48 155
MsG0380016957.01.T01 AT1G54830 63.359 131 38 5 1 125 80 206 2.55e-48 155
MsG0380016957.01.T01 AT1G54830 63.359 131 38 5 1 125 80 206 2.55e-48 155
MsG0380016957.01.T01 AT1G08970 68.103 116 27 4 1 107 90 204 2.22e-47 154
MsG0380016957.01.T01 AT1G08970 68.103 116 27 4 1 107 90 204 2.22e-47 154
MsG0380016957.01.T01 AT1G08970 68.103 116 27 4 1 107 90 204 2.22e-47 154
MsG0380016957.01.T01 AT1G08970 68.103 116 27 4 1 107 90 204 2.22e-47 154
MsG0380016957.01.T01 AT1G56170 66.055 109 32 2 1 107 86 191 4.46e-46 149
MsG0380016957.01.T01 AT1G56170 66.055 109 32 2 1 107 86 191 4.46e-46 149
MsG0380016957.01.T01 AT5G50480 52.817 142 47 6 1 135 64 192 5.62e-38 129
MsG0380016957.01.T01 AT5G27910 45.395 152 69 5 1 148 46 187 6.34e-32 113
MsG0380016957.01.T01 AT5G50490 48.214 112 53 2 1 109 46 155 5.00e-27 100
MsG0380016957.01.T01 AT5G50470 40.132 152 68 6 1 143 73 210 1.11e-25 97.8
MsG0380016957.01.T01 AT5G38140 45.588 68 37 0 1 68 77 144 9.03e-18 76.6

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGGCTTGGCAGTCTGTT+TGG 0.288988 3:-89997186 MsG0380016957.01.T01:CDS
TTGAACTCACCATTCGTTCT+TGG 0.290380 3:-89997468 MsG0380016957.01.T01:CDS
TCCTTATTATTATCCTCCTA+TGG 0.322109 3:-89997281 MsG0380016957.01.T01:CDS
CCCATAGGAGGATAATAATA+AGG 0.322822 3:+89997280 None:intergenic
CAAGCTTTGGCAAAGAGAAT+TGG 0.395526 3:+89997505 None:intergenic
TTATGTTCAGCCGCCTTCTC+AGG 0.407879 3:-89997203 MsG0380016957.01.T01:CDS
AATGAAAAGCTCACAAGCTT+TGG 0.414215 3:+89997492 None:intergenic
TGGTCGTCCCGCTGTTGATC+CGG 0.430949 3:-89997236 MsG0380016957.01.T01:CDS
CCTCCTATGGGACAACCTGC+TGG 0.443243 3:-89997268 MsG0380016957.01.T01:CDS
TTCAGCCGCCTTCTCAGGCT+TGG 0.445274 3:-89997198 MsG0380016957.01.T01:CDS
GTGGATGATGGTTCTTATGC+TGG 0.449967 3:-89997154 MsG0380016957.01.T01:CDS
GTCTGTTTGGCAGACGGGAG+TGG 0.451504 3:-89997173 MsG0380016957.01.T01:CDS
ATATTGCTGCTGCTATTACT+AGG 0.451638 3:-89997402 MsG0380016957.01.T01:CDS
TGGCAGTCTGTTTGGCAGAC+GGG 0.457754 3:-89997178 MsG0380016957.01.T01:CDS
TCTTATGCTGGTGGTGGTGT+TGG 0.460055 3:-89997142 MsG0380016957.01.T01:CDS
TTGGCAGTCTGTTTGGCAGA+CGG 0.463047 3:-89997179 MsG0380016957.01.T01:CDS
CCCGCTGTTGATCCGGCTAC+CGG 0.469158 3:-89997229 MsG0380016957.01.T01:CDS
CCTTATTATTATCCTCCTAT+GGG 0.475192 3:-89997280 MsG0380016957.01.T01:CDS
ATCGAACAAAATGATACCTC+TGG 0.517463 3:+89997095 None:intergenic
ATTCCAGCAGGTTGTCCCAT+AGG 0.521379 3:+89997265 None:intergenic
TGGCAGACGGGAGTGGATGA+TGG 0.530618 3:-89997166 MsG0380016957.01.T01:CDS
GCTGGTGGTGGTGTTGGCAG+TGG 0.530864 3:-89997136 MsG0380016957.01.T01:CDS
CAACCTGCTGGAATGATGAT+TGG 0.531059 3:-89997256 MsG0380016957.01.T01:CDS
TCATCTTTAATCTCGTCTCT+CGG 0.544717 3:+89997346 None:intergenic
TGAACATAAACACCGGTAGC+CGG 0.556443 3:+89997217 None:intergenic
ATTGTTGGTGCTGCTGCTAG+TGG 0.559151 3:-89997307 MsG0380016957.01.T01:CDS
CGACCAATCATCATTCCAGC+AGG 0.560161 3:+89997253 None:intergenic
CCGGTAGCCGGATCAACAGC+GGG 0.566961 3:+89997229 None:intergenic
AGTGGTCAGCACAATGGCGA+TGG 0.568390 3:-89997118 MsG0380016957.01.T01:CDS
GATGGTTCTTATGCTGGTGG+TGG 0.575597 3:-89997148 MsG0380016957.01.T01:CDS
GACTGCCAAGCCTGAGAAGG+CGG 0.581694 3:+89997193 None:intergenic
AGCATGAAGCCAAGAACGAA+TGG 0.590029 3:+89997459 None:intergenic
CCAGCAGGTTGTCCCATAGG+AGG 0.597127 3:+89997268 None:intergenic
ACAGACTGCCAAGCCTGAGA+AGG 0.599594 3:+89997190 None:intergenic
AGCACAATGGCGATGGCCAG+AGG 0.601116 3:-89997111 MsG0380016957.01.T01:CDS
ACCGGTAGCCGGATCAACAG+CGG 0.640253 3:+89997228 None:intergenic
GATGATGGTTCTTATGCTGG+TGG 0.644586 3:-89997151 MsG0380016957.01.T01:CDS
GTTGGCAGTGGTCAGCACAA+TGG 0.663712 3:-89997124 MsG0380016957.01.T01:CDS
AGGCGGCTGAACATAAACAC+CGG 0.726448 3:+89997210 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GACTGATATTTTTGATTTTT+TGG - Chr3:89997254-89997273 MsG0380016957.01.T01:CDS 20.0%
CCTTATTATTATCCTCCTAT+GGG - Chr3:89997354-89997373 MsG0380016957.01.T01:CDS 30.0%
TCCTTATTATTATCCTCCTA+TGG - Chr3:89997353-89997372 MsG0380016957.01.T01:CDS 30.0%
AATGAAAAGCTCACAAGCTT+TGG + Chr3:89997145-89997164 None:intergenic 35.0%
ATATTGCTGCTGCTATTACT+AGG - Chr3:89997232-89997251 MsG0380016957.01.T01:CDS 35.0%
ATCGAACAAAATGATACCTC+TGG + Chr3:89997542-89997561 None:intergenic 35.0%
CCCATAGGAGGATAATAATA+AGG + Chr3:89997357-89997376 None:intergenic 35.0%
TCATCTTTAATCTCGTCTCT+CGG + Chr3:89997291-89997310 None:intergenic 35.0%
TTGAACTCACCATTCGTTCT+TGG - Chr3:89997166-89997185 MsG0380016957.01.T01:CDS 40.0%
! CAAGCTTTGGCAAAGAGAAT+TGG + Chr3:89997132-89997151 None:intergenic 40.0%
AGCATGAAGCCAAGAACGAA+TGG + Chr3:89997178-89997197 None:intergenic 45.0%
CAACCTGCTGGAATGATGAT+TGG - Chr3:89997378-89997397 MsG0380016957.01.T01:CDS 45.0%
TGAACATAAACACCGGTAGC+CGG + Chr3:89997420-89997439 None:intergenic 45.0%
! GATGATGGTTCTTATGCTGG+TGG - Chr3:89997483-89997502 MsG0380016957.01.T01:CDS 45.0%
! GTGGATGATGGTTCTTATGC+TGG - Chr3:89997480-89997499 MsG0380016957.01.T01:CDS 45.0%
AGGCGGCTGAACATAAACAC+CGG + Chr3:89997427-89997446 None:intergenic 50.0%
ATTCCAGCAGGTTGTCCCAT+AGG + Chr3:89997372-89997391 None:intergenic 50.0%
CGACCAATCATCATTCCAGC+AGG + Chr3:89997384-89997403 None:intergenic 50.0%
GATGGTTCTTATGCTGGTGG+TGG - Chr3:89997486-89997505 MsG0380016957.01.T01:CDS 50.0%
TTATGTTCAGCCGCCTTCTC+AGG - Chr3:89997431-89997450 MsG0380016957.01.T01:CDS 50.0%
TTGGCAGTCTGTTTGGCAGA+CGG - Chr3:89997455-89997474 MsG0380016957.01.T01:CDS 50.0%
!! ATTGTTGGTGCTGCTGCTAG+TGG - Chr3:89997327-89997346 MsG0380016957.01.T01:CDS 50.0%
!! TCTTATGCTGGTGGTGGTGT+TGG - Chr3:89997492-89997511 MsG0380016957.01.T01:CDS 50.0%
ACAGACTGCCAAGCCTGAGA+AGG + Chr3:89997447-89997466 None:intergenic 55.0%
AGTGGTCAGCACAATGGCGA+TGG - Chr3:89997516-89997535 MsG0380016957.01.T01:CDS 55.0%
CTCAGGCTTGGCAGTCTGTT+TGG - Chr3:89997448-89997467 MsG0380016957.01.T01:CDS 55.0%
GCTGCTGCTGCTGCTATTGT+TGG - Chr3:89997312-89997331 MsG0380016957.01.T01:CDS 55.0%
GTTGGCAGTGGTCAGCACAA+TGG - Chr3:89997510-89997529 MsG0380016957.01.T01:CDS 55.0%
TGGCAGTCTGTTTGGCAGAC+GGG - Chr3:89997456-89997475 MsG0380016957.01.T01:CDS 55.0%
ACCGGTAGCCGGATCAACAG+CGG + Chr3:89997409-89997428 None:intergenic 60.0%
AGCACAATGGCGATGGCCAG+AGG - Chr3:89997523-89997542 MsG0380016957.01.T01:CDS 60.0%
CCAGCAGGTTGTCCCATAGG+AGG + Chr3:89997369-89997388 None:intergenic 60.0%
CCTCCTATGGGACAACCTGC+TGG - Chr3:89997366-89997385 MsG0380016957.01.T01:CDS 60.0%
GACTGCCAAGCCTGAGAAGG+CGG + Chr3:89997444-89997463 None:intergenic 60.0%
GTCTGTTTGGCAGACGGGAG+TGG - Chr3:89997461-89997480 MsG0380016957.01.T01:CDS 60.0%
TGGCAGACGGGAGTGGATGA+TGG - Chr3:89997468-89997487 MsG0380016957.01.T01:CDS 60.0%
TGGTCGTCCCGCTGTTGATC+CGG - Chr3:89997398-89997417 MsG0380016957.01.T01:CDS 60.0%
TTCAGCCGCCTTCTCAGGCT+TGG - Chr3:89997436-89997455 MsG0380016957.01.T01:CDS 60.0%
CCCGCTGTTGATCCGGCTAC+CGG - Chr3:89997405-89997424 MsG0380016957.01.T01:CDS 65.0%
CCGGTAGCCGGATCAACAGC+GGG + Chr3:89997408-89997427 None:intergenic 65.0%
!! GCTGGTGGTGGTGTTGGCAG+TGG - Chr3:89997498-89997517 MsG0380016957.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 89997087 89997569 89997087 ID=MsG0380016957.01;Name=MsG0380016957.01
Chr3 mRNA 89997087 89997569 89997087 ID=MsG0380016957.01.T01;Parent=MsG0380016957.01;Name=MsG0380016957.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|160
Chr3 exon 89997087 89997569 89997087 ID=MsG0380016957.01.T01:exon:12357;Parent=MsG0380016957.01.T01
Chr3 CDS 89997087 89997569 89997087 ID=MsG0380016957.01.T01:cds;Parent=MsG0380016957.01.T01
Gene Sequence

>MsG0380016957.01.T01

ATGAAAGCTGATGAAGACGTTCGAATGATTTCTGCAGAAGCACCAATTCTCTTTGCCAAAGCTTGTGAGCTTTTCATTCTTGAACTCACCATTCGTTCTTGGCTTCATGCTGAAGAGAATAAACGACGAACTCTTCAGAAAAATGATATTGCTGCTGCTATTACTAGGACTGATATTTTTGATTTTTTGGTTGATATTGTTCCGAGAGACGAGATTAAAGATGAAGCTGCTGCTGCTGCTATTGTTGGTGCTGCTGCTAGTGGTGTTCCTTATTATTATCCTCCTATGGGACAACCTGCTGGAATGATGATTGGTCGTCCCGCTGTTGATCCGGCTACCGGTGTTTATGTTCAGCCGCCTTCTCAGGCTTGGCAGTCTGTTTGGCAGACGGGAGTGGATGATGGTTCTTATGCTGGTGGTGGTGTTGGCAGTGGTCAGCACAATGGCGATGGCCAGAGGTATCATTTTGTTCGATTTTCTTAA

Protein sequence

>MsG0380016957.01.T01

MKADEDVRMISAEAPILFAKACELFILELTIRSWLHAEENKRRTLQKNDIAAAITRTDIFDFLVDIVPRDEIKDEAAAAAIVGAAASGVPYYYPPMGQPAGMMIGRPAVDPATGVYVQPPSQAWQSVWQTGVDDGSYAGGGVGSGQHNGDGQRYHFVRFS*