Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023569.01.T01 | AET05046.1 | 63.478 | 115 | 1 | 1 | 2 | 75 | 23 | 137 | 2.03E-37 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023569.01.T01 | P93092 | 70 | 40 | 12 | 0 | 35 | 74 | 95 | 134 | 2.78E-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023569.01.T01 | G7LIR9 | 63.478 | 115 | 1 | 1 | 2 | 75 | 23 | 137 | 9.69e-38 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048777.01 | MsG0480023569.01 | 0.839769 | 1.299607e-57 | 1.812973e-54 |
| MsG0080048780.01 | MsG0480023569.01 | 0.817307 | 3.528639e-52 | 2.560483e-49 |
| MsG0080048876.01 | MsG0480023569.01 | 0.843886 | 1.063435e-58 | 1.687579e-55 |
| MsG0080049039.01 | MsG0480023569.01 | 0.898091 | 7.451696e-77 | 8.677552e-73 |
| MsG0080049053.01 | MsG0480023569.01 | 0.812021 | 5.226473e-51 | 3.285800e-48 |
| MsG0080049054.01 | MsG0480023569.01 | 0.840470 | 8.529269e-58 | 1.216124e-54 |
| MsG0080049063.01 | MsG0480023569.01 | 0.809466 | 1.866213e-50 | 1.096518e-47 |
| MsG0080049068.01 | MsG0480023569.01 | 0.806115 | 9.619628e-50 | 5.178560e-47 |
| MsG0180001085.01 | MsG0480023569.01 | 0.819298 | 1.250004e-52 | 9.580970e-50 |
| MsG0180001175.01 | MsG0480023569.01 | 0.824973 | 6.038436e-54 | 5.431844e-51 |
| MsG0180001270.01 | MsG0480023569.01 | 0.805073 | 1.591872e-49 | 8.343348e-47 |
| MsG0180003526.01 | MsG0480023569.01 | 0.819189 | 1.323553e-52 | 1.011464e-49 |
| MsG0180003527.01 | MsG0480023569.01 | 0.805449 | 1.327751e-49 | 7.026514e-47 |
| MsG0180003531.01 | MsG0480023569.01 | 0.849929 | 2.357921e-60 | 4.542705e-57 |
| MsG0180004163.01 | MsG0480023569.01 | 0.852566 | 4.244278e-61 | 8.904659e-58 |
| MsG0180004192.01 | MsG0480023569.01 | 0.811588 | 6.493336e-51 | 4.035245e-48 |
| MsG0180004276.01 | MsG0480023569.01 | 0.822564 | 2.214919e-53 | 1.860674e-50 |
| MsG0180004542.01 | MsG0480023569.01 | 0.824494 | 7.829164e-54 | 6.945861e-51 |
| MsG0180004554.01 | MsG0480023569.01 | 0.819353 | 1.214339e-52 | 9.321788e-50 |
| MsG0180004733.01 | MsG0480023569.01 | 0.829053 | 6.384650e-55 | 6.460182e-52 |
| MsG0180004735.01 | MsG0480023569.01 | 0.818905 | 1.535742e-52 | 1.164574e-49 |
| MsG0180004739.01 | MsG0480023569.01 | 0.829030 | 6.465678e-55 | 6.537965e-52 |
| MsG0180005269.01 | MsG0480023569.01 | 0.818153 | 2.273599e-52 | 1.688552e-49 |
| MsG0180005492.01 | MsG0480023569.01 | 0.817201 | 3.727575e-52 | 2.697088e-49 |
| MsG0180005755.01 | MsG0480023569.01 | 0.821656 | 3.595167e-53 | 2.942837e-50 |
| MsG0180005912.01 | MsG0480023569.01 | 0.897725 | 1.063999e-76 | 1.217901e-72 |
| MsG0180005961.01 | MsG0480023569.01 | 0.856409 | 3.281238e-62 | 7.824516e-59 |
| MsG0380012622.01 | MsG0480023569.01 | 0.804095 | 2.546744e-49 | 1.301606e-46 |
| MsG0380012801.01 | MsG0480023569.01 | 0.824579 | 7.476864e-54 | 6.650301e-51 |
| MsG0380014470.01 | MsG0480023569.01 | 0.842750 | 2.137381e-58 | 3.272106e-55 |
| MsG0480018644.01 | MsG0480023569.01 | 0.820758 | 5.790497e-53 | 4.622641e-50 |
| MsG0480019147.01 | MsG0480023569.01 | -0.820155 | 7.966101e-53 | 6.254106e-50 |
| MsG0480019221.01 | MsG0480023569.01 | 0.818079 | 2.363646e-52 | 1.751736e-49 |
| MsG0480019340.01 | MsG0480023569.01 | 0.802240 | 6.161179e-49 | 3.002911e-46 |
| MsG0480019751.01 | MsG0480023569.01 | 0.836485 | 9.102914e-57 | 1.148755e-53 |
| MsG0480019956.01 | MsG0480023569.01 | 0.851933 | 6.425514e-61 | 1.321007e-57 |
| MsG0480020408.01 | MsG0480023569.01 | 0.804108 | 2.530739e-49 | 1.293830e-46 |
| MsG0480020690.01 | MsG0480023569.01 | 0.828913 | 6.902553e-55 | 6.955783e-52 |
| MsG0480020753.01 | MsG0480023569.01 | 0.835688 | 1.450855e-56 | 1.787651e-53 |
| MsG0480020888.01 | MsG0480023569.01 | 0.817941 | 2.539334e-52 | 1.874909e-49 |
| MsG0480021254.01 | MsG0480023569.01 | 0.843673 | 1.212849e-58 | 1.911728e-55 |
| MsG0480021303.01 | MsG0480023569.01 | 0.826040 | 3.374377e-54 | 3.128910e-51 |
| MsG0480021339.01 | MsG0480023569.01 | 0.806950 | 6.412143e-50 | 3.526430e-47 |
| MsG0480021346.01 | MsG0480023569.01 | 0.811297 | 7.511305e-51 | 4.632127e-48 |
| MsG0480021521.01 | MsG0480023569.01 | 0.804101 | 2.538591e-49 | 1.297642e-46 |
| MsG0480021682.01 | MsG0480023569.01 | 0.870676 | 1.219027e-66 | 4.782099e-63 |
| MsG0480021709.01 | MsG0480023569.01 | 0.809478 | 1.854824e-50 | 1.090178e-47 |
| MsG0480021754.01 | MsG0480023569.01 | 0.833807 | 4.314434e-56 | 5.024034e-53 |
| MsG0480021803.01 | MsG0480023569.01 | 0.880977 | 3.517013e-70 | 2.023924e-66 |
| MsG0480022149.01 | MsG0480023569.01 | 0.807277 | 5.468957e-50 | 3.033435e-47 |
| MsG0480022260.01 | MsG0480023569.01 | 0.818329 | 2.075310e-52 | 1.548788e-49 |
| MsG0480022366.01 | MsG0480023569.01 | 0.801547 | 8.552172e-49 | 4.095365e-46 |
| MsG0480023098.01 | MsG0480023569.01 | 0.824449 | 8.025539e-54 | 7.110282e-51 |
| MsG0480023314.01 | MsG0480023569.01 | 0.881652 | 2.008085e-70 | 1.186191e-66 |
| MsG0480023569.01 | MsG0480023590.01 | 0.806419 | 8.302020e-50 | 4.503865e-47 |
| MsG0480023569.01 | MsG0480023661.01 | 0.837786 | 4.232429e-57 | 5.557690e-54 |
| MsG0480023569.01 | MsG0580024327.01 | 0.814229 | 1.713162e-51 | 1.143021e-48 |
| MsG0480023569.01 | MsG0580025411.01 | 0.803336 | 3.660630e-49 | 1.834657e-46 |
| MsG0480023569.01 | MsG0580025828.01 | 0.808646 | 2.795536e-50 | 1.607070e-47 |
| MsG0480023569.01 | MsG0580027394.01 | 0.818100 | 2.337185e-52 | 1.733177e-49 |
| MsG0480023569.01 | MsG0580027887.01 | 0.855002 | 8.449399e-62 | 1.922639e-58 |
| MsG0480023569.01 | MsG0580027983.01 | 0.814679 | 1.362722e-51 | 9.203342e-49 |
| MsG0480023569.01 | MsG0580028059.01 | 0.805670 | 1.193619e-49 | 6.352641e-47 |
| MsG0480023569.01 | MsG0580028335.01 | 0.825971 | 3.503992e-54 | 3.242837e-51 |
| MsG0480023569.01 | MsG0580029753.01 | 0.803059 | 4.177121e-49 | 2.078667e-46 |
| MsG0480023569.01 | MsG0580029878.01 | 0.879683 | 1.020108e-69 | 5.582097e-66 |
| MsG0480023569.01 | MsG0680030627.01 | 0.858559 | 7.580372e-63 | 1.942372e-59 |
| MsG0480023569.01 | MsG0680031715.01 | 0.825319 | 5.002280e-54 | 4.544241e-51 |
| MsG0480023569.01 | MsG0680032238.01 | 0.801035 | 1.088868e-48 | 5.147710e-46 |
| MsG0480023569.01 | MsG0680032554.01 | 0.809160 | 2.171017e-50 | 1.265268e-47 |
| MsG0480023569.01 | MsG0680032559.01 | 0.813287 | 2.763062e-51 | 1.796951e-48 |
| MsG0480023569.01 | MsG0680032651.01 | 0.811616 | 6.405845e-51 | 3.983726e-48 |
| MsG0480023569.01 | MsG0680033377.01 | 0.823674 | 1.220004e-53 | 1.057598e-50 |
| MsG0480023569.01 | MsG0680034064.01 | 0.805471 | 1.313736e-49 | 6.956242e-47 |
| MsG0480023569.01 | MsG0680035731.01 | 0.839194 | 1.833232e-57 | 2.512741e-54 |
| MsG0480023569.01 | MsG0680035789.01 | 0.817236 | 3.660849e-52 | 2.651288e-49 |
| MsG0480023569.01 | MsG0680035791.01 | 0.853256 | 2.694185e-61 | 5.786255e-58 |
| MsG0480023569.01 | MsG0780036715.01 | 0.816757 | 4.691321e-52 | 3.353387e-49 |
| MsG0480023569.01 | MsG0780037119.01 | 0.809439 | 1.891388e-50 | 1.110520e-47 |
| MsG0480023569.01 | MsG0780037184.01 | 0.818164 | 2.260953e-52 | 1.679648e-49 |
| MsG0480023569.01 | MsG0780038351.01 | 0.810668 | 1.028172e-50 | 6.235959e-48 |
| MsG0480023569.01 | MsG0780038616.01 | 0.850428 | 1.708768e-60 | 3.346317e-57 |
| MsG0480023569.01 | MsG0780038784.01 | 0.835043 | 2.111434e-56 | 2.551461e-53 |
| MsG0480023569.01 | MsG0780038807.01 | 0.863716 | 2.040928e-64 | 6.248920e-61 |
| MsG0480023569.01 | MsG0780039422.01 | 0.818512 | 1.885429e-52 | 1.414230e-49 |
| MsG0480023569.01 | MsG0780039675.01 | 0.849159 | 3.865899e-60 | 7.264423e-57 |
| MsG0480023569.01 | MsG0780039746.01 | 0.883473 | 4.354316e-71 | 2.764409e-67 |
| MsG0480023569.01 | MsG0780039853.01 | 0.815875 | 7.387239e-52 | 5.154318e-49 |
| MsG0480023569.01 | MsG0780040251.01 | 0.823354 | 1.449470e-53 | 1.244957e-50 |
| MsG0480023569.01 | MsG0780040284.01 | 0.828859 | 7.113825e-55 | 7.157004e-52 |
| MsG0480023569.01 | MsG0780040331.01 | 0.872595 | 2.818585e-67 | 1.186347e-63 |
| MsG0480023569.01 | MsG0780040884.01 | 0.834797 | 2.434920e-56 | 2.920470e-53 |
| MsG0480023569.01 | MsG0780040928.01 | 0.808089 | 3.676062e-50 | 2.082681e-47 |
| MsG0480023569.01 | MsG0780040972.01 | 0.812406 | 4.308455e-51 | 2.737208e-48 |
| MsG0480023569.01 | MsG0780041138.01 | 0.804796 | 1.818878e-49 | 9.465643e-47 |
| MsG0480023569.01 | MsG0780041174.01 | 0.828968 | 6.694532e-55 | 6.757076e-52 |
| MsG0480023569.01 | MsG0780041480.01 | 0.884540 | 1.756930e-71 | 1.163643e-67 |
| MsG0480023569.01 | MsG0880042475.01 | 0.851751 | 7.236969e-61 | 1.479546e-57 |
| MsG0480023569.01 | MsG0880042890.01 | 0.839420 | 1.601562e-57 | 2.210626e-54 |
| MsG0480023569.01 | MsG0880043814.01 | 0.818162 | 2.264029e-52 | 1.681807e-49 |
| MsG0480023569.01 | MsG0880044228.01 | 0.838808 | 2.307811e-57 | 3.126110e-54 |
| MsG0480023569.01 | MsG0880044392.01 | 0.834495 | 2.900783e-56 | 3.448423e-53 |
| MsG0480023569.01 | MsG0880044414.01 | 0.871917 | 4.742406e-67 | 1.946072e-63 |
| MsG0480023569.01 | MsG0880044604.01 | 0.815697 | 8.094177e-52 | 5.620374e-49 |
| MsG0480023569.01 | MsG0880045101.01 | 0.846174 | 2.562538e-59 | 4.372953e-56 |
| MsG0480023569.01 | MsG0880046104.01 | 0.803366 | 3.608659e-49 | 1.809972e-46 |
| MsG0480023569.01 | MsG0880046267.01 | 0.853556 | 2.210909e-61 | 4.796013e-58 |
| MsG0480023569.01 | MsG0880046791.01 | 0.813136 | 2.981236e-51 | 1.930848e-48 |
| MsG0480023569.01 | MsG0880047325.01 | 0.807960 | 3.916091e-50 | 2.211089e-47 |
| MsG0480023569.01 | MsG0880047567.01 | 0.837712 | 4.421501e-57 | 5.792605e-54 |
| MsG0480023569.01 | MsG0880047616.01 | 0.839789 | 1.284484e-57 | 1.793072e-54 |
| MsG0380014940.01 | MsG0480023569.01 | 0.837630 | 4.640683e-57 | 6.064340e-54 |
| MsG0380015081.01 | MsG0480023569.01 | 0.801568 | 8.468667e-49 | 4.057558e-46 |
| MsG0380015356.01 | MsG0480023569.01 | 0.842600 | 2.342648e-58 | 3.569996e-55 |
| MsG0380015399.01 | MsG0480023569.01 | 0.801492 | 8.777634e-49 | 4.197643e-46 |
| MsG0380015603.01 | MsG0480023569.01 | 0.863613 | 2.197668e-64 | 6.703016e-61 |
| MsG0380015693.01 | MsG0480023569.01 | 0.839904 | 1.198676e-57 | 1.679398e-54 |
| MsG0380015742.01 | MsG0480023569.01 | 0.856342 | 3.433483e-62 | 8.169367e-59 |
| MsG0380015743.01 | MsG0480023569.01 | 0.853381 | 2.481794e-61 | 5.352188e-58 |
| MsG0380016134.01 | MsG0480023569.01 | 0.809150 | 2.180883e-50 | 1.270697e-47 |
| MsG0380016137.01 | MsG0480023569.01 | 0.818956 | 1.495038e-52 | 1.135334e-49 |
| MsG0380016192.01 | MsG0480023569.01 | 0.820726 | 5.891140e-53 | 4.698555e-50 |
| MsG0380016544.01 | MsG0480023569.01 | 0.830166 | 3.423139e-55 | 3.578345e-52 |
| MsG0380016836.01 | MsG0480023569.01 | 0.809323 | 2.002400e-50 | 1.172107e-47 |
| MsG0380016957.01 | MsG0480023569.01 | 0.866523 | 2.679057e-65 | 9.057304e-62 |
| MsG0380017028.01 | MsG0480023569.01 | 0.884478 | 1.853092e-71 | 1.224207e-67 |
| MsG0380017100.01 | MsG0480023569.01 | 0.825517 | 4.490964e-54 | 4.102566e-51 |
| MsG0380017336.01 | MsG0480023569.01 | 0.838547 | 2.695413e-57 | 3.622270e-54 |
| MsG0380017703.01 | MsG0480023569.01 | 0.854369 | 1.288998e-61 | 2.871944e-58 |
| MsG0380017838.01 | MsG0480023569.01 | 0.813030 | 3.145390e-51 | 2.031435e-48 |
| MsG0380018027.01 | MsG0480023569.01 | 0.840353 | 9.151183e-58 | 1.300051e-54 |
| MsG0380018061.01 | MsG0480023569.01 | 0.803074 | 4.146738e-49 | 2.064325e-46 |
| MsG0380018069.01 | MsG0480023569.01 | 0.802282 | 6.042239e-49 | 2.948077e-46 |
| MsG0280006805.01 | MsG0480023569.01 | 0.865100 | 7.544702e-65 | 2.425334e-61 |
| MsG0280006825.01 | MsG0480023569.01 | 0.827605 | 1.426921e-54 | 1.384105e-51 |
| MsG0280007502.01 | MsG0480023569.01 | 0.849605 | 2.905592e-60 | 5.537927e-57 |
| MsG0280007831.01 | MsG0480023569.01 | 0.863476 | 2.422970e-64 | 7.354104e-61 |
| MsG0280008081.01 | MsG0480023569.01 | 0.804517 | 2.079625e-49 | 1.074394e-46 |
| MsG0280008388.01 | MsG0480023569.01 | 0.835468 | 1.648502e-56 | 2.017681e-53 |
| MsG0280008394.01 | MsG0480023569.01 | 0.809997 | 1.434543e-50 | 8.548443e-48 |
| MsG0280008451.01 | MsG0480023569.01 | 0.845653 | 3.551996e-59 | 5.961110e-56 |
| MsG0280008591.01 | MsG0480023569.01 | 0.888548 | 5.356902e-73 | 4.168692e-69 |
| MsG0280010016.01 | MsG0480023569.01 | 0.806291 | 8.833218e-50 | 4.776561e-47 |
| MsG0280010376.01 | MsG0480023569.01 | 0.845322 | 4.366546e-59 | 7.249894e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023569.01.T01 | MTR_8g099920 | 63.478 | 115 | 1 | 1 | 2 | 75 | 23 | 137 | 2.46e-41 | 132 |
| MsG0480023569.01.T01 | MTR_8g099920 | 62.393 | 117 | 1 | 1 | 2 | 75 | 23 | 139 | 5.09e-41 | 131 |
| MsG0480023569.01.T01 | MTR_8g099920 | 91.429 | 35 | 3 | 0 | 2 | 36 | 23 | 57 | 3.95e-18 | 72.4 |
| MsG0480023569.01.T01 | MTR_2g020360 | 60.000 | 40 | 16 | 0 | 35 | 74 | 99 | 138 | 3.25e-12 | 57.8 |
| MsG0480023569.01.T01 | MTR_2g020360 | 60.000 | 40 | 16 | 0 | 35 | 74 | 99 | 138 | 3.32e-12 | 57.8 |
| MsG0480023569.01.T01 | MTR_2g020360 | 60.000 | 40 | 16 | 0 | 35 | 74 | 99 | 138 | 5.00e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023569.01.T01 | AT4G25050 | 66.667 | 42 | 14 | 0 | 33 | 74 | 102 | 143 | 5.08e-15 | 65.5 |
| MsG0480023569.01.T01 | AT4G25050 | 67.500 | 40 | 13 | 0 | 35 | 74 | 92 | 131 | 7.57e-14 | 62.0 |
| MsG0480023569.01.T01 | AT5G27200 | 60.000 | 40 | 16 | 0 | 35 | 74 | 98 | 137 | 5.48e-11 | 54.7 |
Find 38 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATCATCCTCAATATTA+AGG | 0.281641 | 4:+87027612 | None:intergenic |
| AATACATTGTGAACTGAGTT+AGG | 0.285621 | 4:-87027486 | MsG0480023569.01.T01:three_prime_UTR |
| ACATTAATATGTTAAGGTTT+TGG | 0.288301 | 4:-87028486 | MsG0480023569.01.T01:five_prime_UTR |
| TGTGCTTATGCGTATTCTTT+TGG | 0.292912 | 4:-87027427 | MsG0480023569.01.T01:three_prime_UTR |
| TCTTTCAGAACTTCAAGTTT+TGG | 0.316075 | 4:-87028202 | MsG0480023569.01.T01:CDS |
| GAGTGTCCAATTGATAATTT+AGG | 0.331274 | 4:-87028574 | MsG0480023569.01.T01:five_prime_UTR |
| AAAGAAAGATTAAGCTTAAG+AGG | 0.355256 | 4:-87027534 | MsG0480023569.01.T01:exon |
| GGAAATAGTGATGGGTTTAG+AGG | 0.377032 | 4:-87027645 | MsG0480023569.01.T01:CDS |
| CCAACACCTAAATTATCAAT+TGG | 0.386855 | 4:+87028568 | None:intergenic |
| ACATACACATTAATATGTTA+AGG | 0.416034 | 4:-87028492 | MsG0480023569.01.T01:five_prime_UTR |
| TATAGAGAAATTTCCAAAAG+TGG | 0.421968 | 4:+87028646 | None:intergenic |
| CCAATTGATAATTTAGGTGT+TGG | 0.434525 | 4:-87028568 | MsG0480023569.01.T01:five_prime_UTR |
| ATAAACAACAATTATCGTAT+AGG | 0.442319 | 4:-87028413 | MsG0480023569.01.T01:five_prime_UTR |
| ATGGAACATTGCCATAATCT+TGG | 0.469205 | 4:+87027271 | None:intergenic |
| TACGCATAAGCACAAATAAG+AGG | 0.474753 | 4:+87027436 | None:intergenic |
| AACTTTCTAAAACCCACATT+AGG | 0.476053 | 4:+87027241 | None:intergenic |
| CAAATCAGCTGCTTGTTGAA+TGG | 0.477053 | 4:+87027574 | None:intergenic |
| TATAGCATAGTTACATTGGT+AGG | 0.481531 | 4:-87028534 | MsG0480023569.01.T01:five_prime_UTR |
| TTCCATCCTTGTGCCTAATG+TGG | 0.493817 | 4:-87027254 | MsG0480023569.01.T01:three_prime_UTR |
| AGAGTTTAACCTTAATATTG+AGG | 0.511328 | 4:-87027621 | MsG0480023569.01.T01:CDS |
| ATTGTTATTTGTTGCAAGCA+TGG | 0.532884 | 4:-87027364 | MsG0480023569.01.T01:three_prime_UTR |
| AGTGGTTAAAACATACATGC+AGG | 0.539419 | 4:-87028227 | MsG0480023569.01.T01:exon |
| ATACATTGTGAACTGAGTTA+GGG | 0.539752 | 4:-87027485 | MsG0480023569.01.T01:three_prime_UTR |
| TCCATCCTTGTGCCTAATGT+GGG | 0.545236 | 4:-87027253 | MsG0480023569.01.T01:three_prime_UTR |
| AGTATATAGCATAGTTACAT+TGG | 0.548259 | 4:-87028538 | MsG0480023569.01.T01:five_prime_UTR |
| AATTAAACGCACGGACGTGA+AGG | 0.553945 | 4:-87028596 | MsG0480023569.01.T01:five_prime_UTR |
| ATTAAATGCATGAAATGACT+TGG | 0.565346 | 4:-87028693 | MsG0480023569.01.T01:five_prime_UTR |
| AAACGAGAAGATCTCAAAGA+AGG | 0.566334 | 4:+87028145 | None:intergenic |
| ACAATTTAACCAAGTTACAA+TGG | 0.568659 | 4:+87027382 | None:intergenic |
| GTGGCAGGTGGAAATAGTGA+TGG | 0.572304 | 4:-87027654 | MsG0480023569.01.T01:intron |
| TGAACCCAATGGAAGCTCTA+CGG | 0.572437 | 4:-87027511 | MsG0480023569.01.T01:three_prime_UTR |
| ACCCACATTAGGCACAAGGA+TGG | 0.589368 | 4:+87027252 | None:intergenic |
| ATGAAAATACTTATGAACAA+CGG | 0.607432 | 4:-87028299 | MsG0480023569.01.T01:five_prime_UTR |
| AGCTTAAGAGGTGAACCCAA+TGG | 0.610757 | 4:-87027522 | MsG0480023569.01.T01:three_prime_UTR |
| TAAAACCCACATTAGGCACA+AGG | 0.633907 | 4:+87027248 | None:intergenic |
| TGGCAGGTGGAAATAGTGAT+GGG | 0.642802 | 4:-87027653 | MsG0480023569.01.T01:intron |
| ACACGTGCAAATTAAACGCA+CGG | 0.653562 | 4:-87028605 | MsG0480023569.01.T01:five_prime_UTR |
| TCAAACTTACTGCACAAATG+CGG | 0.672194 | 4:+87028122 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTTAAAAGATTC+AGG | - | Chr4:87027946-87027965 | MsG0480023569.01.T01:intron | 10.0% |
| !!! | ATCATCAGTAAATTTATTTT+TGG | - | Chr4:87028528-87028547 | MsG0480023569.01.T01:five_prime_UTR | 15.0% |
| !!! | GTTTTAATACTTTTTTTATG+AGG | - | Chr4:87027710-87027729 | MsG0480023569.01.T01:intron | 15.0% |
| !! | AAAGTCAAAATGAATTTCAA+AGG | + | Chr4:87027894-87027913 | None:intergenic | 20.0% |
| !! | AAGTCAAAATGAATTTCAAA+GGG | + | Chr4:87027893-87027912 | None:intergenic | 20.0% |
| !! | ACATACACATTAATATGTTA+AGG | - | Chr4:87027495-87027514 | MsG0480023569.01.T01:three_prime_UTR | 20.0% |
| !! | ATAAACAACAATTATCGTAT+AGG | - | Chr4:87027574-87027593 | MsG0480023569.01.T01:CDS | 20.0% |
| !! | ATGAAAATACTTATGAACAA+CGG | - | Chr4:87027688-87027707 | MsG0480023569.01.T01:intron | 20.0% |
| !!! | AAAAAATGTTACTATGCTTT+TGG | - | Chr4:87027264-87027283 | MsG0480023569.01.T01:three_prime_UTR | 20.0% |
| !!! | ACATTAATATGTTAAGGTTT+TGG | - | Chr4:87027501-87027520 | MsG0480023569.01.T01:three_prime_UTR | 20.0% |
| !!! | AGTGAAAATTTTAATTTGAG+TGG | - | Chr4:87027742-87027761 | MsG0480023569.01.T01:intron | 20.0% |
| !!! | ATATTTTTTCCATTGTAACT+TGG | - | Chr4:87028596-87028615 | MsG0480023569.01.T01:five_prime_UTR | 20.0% |
| !!! | ATTATTGGTTTTGATTTTGA+TGG | - | Chr4:87028214-87028233 | MsG0480023569.01.T01:CDS | 20.0% |
| !!! | TGATTGATTCTTAATTAATG+TGG | - | Chr4:87028314-87028333 | MsG0480023569.01.T01:five_prime_UTR | 20.0% |
| ! | AAAAAAATATTGTACTCCGT+TGG | - | Chr4:87028147-87028166 | MsG0480023569.01.T01:CDS | 25.0% |
| ! | AAAACCAATAATGTTCATTC+AGG | + | Chr4:87028206-87028225 | None:intergenic | 25.0% |
| ! | AAAGAAAGATTAAGCTTAAG+AGG | - | Chr4:87028453-87028472 | MsG0480023569.01.T01:five_prime_UTR | 25.0% |
| ! | ACAATTTAACCAAGTTACAA+TGG | + | Chr4:87028608-87028627 | None:intergenic | 25.0% |
| ! | AGAGTTTAACCTTAATATTG+AGG | - | Chr4:87028366-87028385 | MsG0480023569.01.T01:five_prime_UTR | 25.0% |
| ! | AGTATATAGCATAGTTACAT+TGG | - | Chr4:87027449-87027468 | MsG0480023569.01.T01:three_prime_UTR | 25.0% |
| ! | ATTAAATGCATGAAATGACT+TGG | - | Chr4:87027294-87027313 | MsG0480023569.01.T01:three_prime_UTR | 25.0% |
| ! | CATTATCATCCTCAATATTA+AGG | + | Chr4:87028378-87028397 | None:intergenic | 25.0% |
| ! | TATAGAGAAATTTCCAAAAG+TGG | + | Chr4:87027344-87027363 | None:intergenic | 25.0% |
| !! | GAAACTAAATTTTCAGAACT+TGG | - | Chr4:87028047-87028066 | MsG0480023569.01.T01:intron | 25.0% |
| !!! | AAGCATGTTATTTGCTTTTT+AGG | - | Chr4:87028693-87028712 | MsG0480023569.01.T01:five_prime_UTR | 25.0% |
| !!! | AATACTTTTTTTATGAGGTG+AGG | - | Chr4:87027715-87027734 | MsG0480023569.01.T01:intron | 25.0% |
| !!! | AGTTCTGAAAATTTAGTTTC+TGG | + | Chr4:87028047-87028066 | None:intergenic | 25.0% |
| !!! | GTTCTGAAAATTTAGTTTCT+GGG | + | Chr4:87028046-87028065 | None:intergenic | 25.0% |
| !!! | TCCAAAAGTGGTTTATTTTT+CGG | + | Chr4:87027332-87027351 | None:intergenic | 25.0% |
| !!! | TGACTTTTAAAAAACTTGCA+AGG | - | Chr4:87027907-87027926 | MsG0480023569.01.T01:intron | 25.0% |
| !!! | TGTGTTGTTGTTTTTCTATT+TGG | - | Chr4:87027551-87027570 | MsG0480023569.01.T01:CDS | 25.0% |
| AATACATTGTGAACTGAGTT+AGG | - | Chr4:87028501-87028520 | MsG0480023569.01.T01:five_prime_UTR | 30.0% | |
| ATACATTGTGAACTGAGTTA+GGG | - | Chr4:87028502-87028521 | MsG0480023569.01.T01:five_prime_UTR | 30.0% | |
| CCAACACCTAAATTATCAAT+TGG | + | Chr4:87027422-87027441 | None:intergenic | 30.0% | |
| TATAGCATAGTTACATTGGT+AGG | - | Chr4:87027453-87027472 | MsG0480023569.01.T01:three_prime_UTR | 30.0% | |
| TGATTCTTAATTAATGTGGC+AGG | - | Chr4:87028318-87028337 | MsG0480023569.01.T01:five_prime_UTR | 30.0% | |
| ! | CCAATTGATAATTTAGGTGT+TGG | - | Chr4:87027419-87027438 | MsG0480023569.01.T01:three_prime_UTR | 30.0% |
| ! | GAGTGTCCAATTGATAATTT+AGG | - | Chr4:87027413-87027432 | MsG0480023569.01.T01:three_prime_UTR | 30.0% |
| ! | TCCGAAAAATAAACCACTTT+TGG | - | Chr4:87027328-87027347 | MsG0480023569.01.T01:three_prime_UTR | 30.0% |
| !! | ATTGTTATTTGTTGCAAGCA+TGG | - | Chr4:87028623-87028642 | MsG0480023569.01.T01:five_prime_UTR | 30.0% |
| !! | TCTTTCAGAACTTCAAGTTT+TGG | - | Chr4:87027785-87027804 | MsG0480023569.01.T01:intron | 30.0% |
| !!! | TTTGATTTTGATGGTGCATT+TGG | - | Chr4:87028223-87028242 | MsG0480023569.01.T01:exon | 30.0% |
| AAACGAGAAGATCTCAAAGA+AGG | + | Chr4:87027845-87027864 | None:intergenic | 35.0% | |
| AGTGGTTAAAACATACATGC+AGG | - | Chr4:87027760-87027779 | MsG0480023569.01.T01:intron | 35.0% | |
| ATGGAACATTGCCATAATCT+TGG | + | Chr4:87028719-87028738 | None:intergenic | 35.0% | |
| GAACTTGACCTTATAACCAA+CGG | + | Chr4:87028166-87028185 | None:intergenic | 35.0% | |
| GACACCTGAATGAACATTAT+TGG | - | Chr4:87028199-87028218 | MsG0480023569.01.T01:CDS | 35.0% | |
| GTTGTTTATGCTAGAGCTAT+GGG | - | Chr4:87028280-87028299 | MsG0480023569.01.T01:five_prime_UTR | 35.0% | |
| TACGCATAAGCACAAATAAG+AGG | + | Chr4:87028554-87028573 | None:intergenic | 35.0% | |
| TCAAACTTACTGCACAAATG+CGG | + | Chr4:87027868-87027887 | None:intergenic | 35.0% | |
| TTCTTAATTAATGTGGCAGG+TGG | - | Chr4:87028321-87028340 | MsG0480023569.01.T01:five_prime_UTR | 35.0% | |
| ! | ATTGTACTCCGTTGGTTATA+AGG | - | Chr4:87028155-87028174 | MsG0480023569.01.T01:CDS | 35.0% |
| ! | ATTTAGTTTCTGGGTAAGCT+TGG | + | Chr4:87028037-87028056 | None:intergenic | 35.0% |
| ! | CTTATGCGTATTCTTTTGGA+AGG | - | Chr4:87028564-87028583 | MsG0480023569.01.T01:five_prime_UTR | 35.0% |
| ! | TGTGCTTATGCGTATTCTTT+TGG | - | Chr4:87028560-87028579 | MsG0480023569.01.T01:five_prime_UTR | 35.0% |
| ! | TGTTGTTTATGCTAGAGCTA+TGG | - | Chr4:87028279-87028298 | MsG0480023569.01.T01:five_prime_UTR | 35.0% |
| !! | AAGTTTTGGTGCAATTTCTG+TGG | - | Chr4:87027799-87027818 | MsG0480023569.01.T01:intron | 35.0% |
| !! | AGTTTTGGTGCAATTTCTGT+GGG | - | Chr4:87027800-87027819 | MsG0480023569.01.T01:intron | 35.0% |
| !! | TGCTTTTTAGGCCAAGATTA+TGG | - | Chr4:87028705-87028724 | MsG0480023569.01.T01:five_prime_UTR | 35.0% |
| ACACGTGCAAATTAAACGCA+CGG | - | Chr4:87027382-87027401 | MsG0480023569.01.T01:three_prime_UTR | 40.0% | |
| ! | ATTTTCCGTAGAGCTTCCAT+TGG | + | Chr4:87028484-87028503 | None:intergenic | 40.0% |
| ! | CAAATCAGCTGCTTGTTGAA+TGG | + | Chr4:87028416-87028435 | None:intergenic | 40.0% |
| ! | TTTTCCGTAGAGCTTCCATT+GGG | + | Chr4:87028483-87028502 | None:intergenic | 40.0% |
| !! | GGAAATAGTGATGGGTTTAG+AGG | - | Chr4:87028342-87028361 | MsG0480023569.01.T01:five_prime_UTR | 40.0% |
| AATTAAACGCACGGACGTGA+AGG | - | Chr4:87027391-87027410 | MsG0480023569.01.T01:three_prime_UTR | 45.0% | |
| AGCTTAAGAGGTGAACCCAA+TGG | - | Chr4:87028465-87028484 | MsG0480023569.01.T01:five_prime_UTR | 45.0% | |
| TCCATCCTTGTGCCTAATGT+GGG | - | Chr4:87028734-87028753 | MsG0480023569.01.T01:five_prime_UTR | 45.0% | |
| TGAACCCAATGGAAGCTCTA+CGG | - | Chr4:87028476-87028495 | MsG0480023569.01.T01:five_prime_UTR | 45.0% | |
| TGGCAGGTGGAAATAGTGAT+GGG | - | Chr4:87028334-87028353 | MsG0480023569.01.T01:five_prime_UTR | 45.0% | |
| TTCCATCCTTGTGCCTAATG+TGG | - | Chr4:87028733-87028752 | MsG0480023569.01.T01:five_prime_UTR | 45.0% | |
| TTCTGGGTAAGCTTGGTTTC+AGG | + | Chr4:87028030-87028049 | None:intergenic | 45.0% | |
| !!! | TGTTTTATATATATATTTAT+TGG | - | Chr4:87028656-87028675 | MsG0480023569.01.T01:five_prime_UTR | 5.0% |
| ACCCACATTAGGCACAAGGA+TGG | + | Chr4:87028738-87028757 | None:intergenic | 50.0% | |
| GTGGCAGGTGGAAATAGTGA+TGG | - | Chr4:87028333-87028352 | MsG0480023569.01.T01:five_prime_UTR | 50.0% | |
| TCAGGTGTCAGCTCAGTGAT+AGG | + | Chr4:87028188-87028207 | None:intergenic | 50.0% | |
| GCTCACTTCTCCACTGTCTC+AGG | + | Chr4:87027977-87027996 | None:intergenic | 55.0% | |
| TCAGGCACAACCTGAGACAG+TGG | - | Chr4:87027964-87027983 | MsG0480023569.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 87027251 | 87028758 | 87027251 | ID=MsG0480023569.01;Name=MsG0480023569.01 |
| Chr4 | mRNA | 87027251 | 87028758 | 87027251 | ID=MsG0480023569.01.T01;Parent=MsG0480023569.01;Name=MsG0480023569.01.T01;_AED=0.23;_eAED=0.23;_QI=525|0|1|1|1|1|2|293|75 |
| Chr4 | exon | 87027251 | 87027669 | 87027251 | ID=MsG0480023569.01.T01:exon:3204;Parent=MsG0480023569.01.T01 |
| Chr4 | exon | 87028132 | 87028758 | 87028132 | ID=MsG0480023569.01.T01:exon:3203;Parent=MsG0480023569.01.T01 |
| Chr4 | five_prime_UTR | 87028234 | 87028758 | 87028234 | ID=MsG0480023569.01.T01:five_prime_utr;Parent=MsG0480023569.01.T01 |
| Chr4 | CDS | 87028132 | 87028233 | 87028132 | ID=MsG0480023569.01.T01:cds;Parent=MsG0480023569.01.T01 |
| Chr4 | CDS | 87027544 | 87027669 | 87027544 | ID=MsG0480023569.01.T01:cds;Parent=MsG0480023569.01.T01 |
| Chr4 | three_prime_UTR | 87027251 | 87027543 | 87027251 | ID=MsG0480023569.01.T01:three_prime_utr;Parent=MsG0480023569.01.T01 |
| Gene Sequence |
| Protein sequence |
