AlfalfaGEDB | MsG0180003531.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003531.01

KEGG: -
GO: GO:0003700 GO:0006352 GO:0006355
PFAM: PF04539 PF04542 PF04545

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003531.01.T01	XP_003590454.2	84.22	583	23	2	1	536	1	561	0	967

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003531.01.T01	Q9LD95	44.112	535	230	8	54	536	30	547	3.38E-123	375

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003531.01.T01	G7IA88	84.220	583	23	2	1	536	1	561	0.0	967

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0180003531.01	0.803792	2.943309e-49	1.492621e-46
MsG0080047955.01	MsG0180003531.01	0.821856	3.231894e-53	2.660698e-50
MsG0080047956.01	MsG0180003531.01	0.810199	1.298268e-50	7.776355e-48
MsG0080048528.01	MsG0180003531.01	0.821384	4.154994e-53	3.375221e-50
MsG0080048727.01	MsG0180003531.01	0.821497	3.912618e-53	3.188310e-50
MsG0080048773.01	MsG0180003531.01	0.820734	5.863851e-53	4.677915e-50
MsG0080048777.01	MsG0180003531.01	0.848215	7.063819e-60	1.286850e-56
MsG0080048780.01	MsG0180003531.01	0.875033	4.237813e-68	1.950219e-64
MsG0080048801.01	MsG0180003531.01	0.854367	1.290421e-61	2.874983e-58
MsG0080048820.01	MsG0180003531.01	0.810226	1.280485e-50	7.675344e-48

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003531.01.T01	MTR_1g063850	84.220	583	23	2	1	536	1	561	0.0	967
MsG0180003531.01.T01	MTR_7g101840	48.736	554	203	9	52	536	16	557	8.08e-162	472
MsG0180003531.01.T01	MTR_3g118290	29.917	361	201	3	218	531	214	569	4.64e-42	159
MsG0180003531.01.T01	MTR_1g007370	27.160	324	175	6	266	534	232	549	4.92e-25	109
MsG0180003531.01.T01	MTR_3g033420	26.115	314	179	4	267	532	94	402	3.94e-19	90.1
MsG0180003531.01.T01	MTR_3g033150	30.263	152	106	0	381	532	2	153	1.04e-15	75.1
MsG0180003531.01.T01	MTR_3g090900	24.828	290	162	5	283	520	216	501	1.59e-14	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003531.01.T01	AT2G36990	44.112	535	230	8	54	536	30	547	3.45e-124	375
MsG0180003531.01.T01	AT1G08540	33.523	352	178	7	218	520	212	556	7.13e-48	175
MsG0180003531.01.T01	AT3G53920	25.088	283	164	2	286	520	275	557	1.76e-24	107
MsG0180003531.01.T01	AT5G24120	26.702	382	204	9	213	520	125	504	2.53e-18	88.6
MsG0180003531.01.T01	AT5G24120	26.702	382	204	9	213	520	125	504	2.53e-18	88.6
MsG0180003531.01.T01	AT5G24120	26.702	382	204	9	213	520	125	504	2.53e-18	88.6
MsG0180003531.01.T01	AT5G13730	26.316	285	160	2	283	520	122	403	1.42e-15	79.3

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Find 136 sgRNAs with CRISPR-Local

Find 382 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAATTGTACATGGAAG+AGG	0.496632	1:-63805172	MsG0180003531.01.T01:CDS
AAATACAACGCAGATGGATA+GGG	0.547592	1:-63807608	MsG0180003531.01.T01:CDS
AACGTTAAATACAACGCAGA+TGG	0.616452	1:-63807614	MsG0180003531.01.T01:CDS
AAGAAATTGTACATGGAAGA+GGG	0.576209	1:-63805171	MsG0180003531.01.T01:CDS
AAGAAGTGTCTTGTTGGTCA+AGG	0.579678	1:-63803639	MsG0180003531.01.T01:CDS
AAGAGATCAAAGTATTAAAC+AGG	0.428369	1:-63803415	MsG0180003531.01.T01:three_prime_UTR
AAGAGCAGTGTGATGAGTAT+AGG	0.500553	1:-63808146	MsG0180003531.01.T01:CDS
AAGCTCAAGAAGTGTCTTGT+TGG	0.438338	1:-63803645	MsG0180003531.01.T01:CDS
AAGTAAAGGTTCAGCCGAAC+TGG	0.401281	1:+63807069	None:intergenic
AATATCAATTAAAGCTGACG+AGG	0.690371	1:-63807233	MsG0180003531.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAATTGTACATGGAAG+AGG	-	Chr1:63807655-63807674	MsG0180003531.01.T01:intron	30.0%
AAAGTTGTGCTTTCATTGAA+TGG	+	Chr1:63804979-63804998	None:intergenic	30.0%
AAATCAAATGGCAAGAAAAG+TGG	+	Chr1:63804350-63804369	None:intergenic	30.0%
AAGAAATTGTACATGGAAGA+GGG	-	Chr1:63807656-63807675	MsG0180003531.01.T01:intron	30.0%
AATATCAATTAAAGCTGACG+AGG	-	Chr1:63805594-63805613	MsG0180003531.01.T01:intron	30.0%
ACAAAAATTAAGGTAGTTCG+TGG	-	Chr1:63807039-63807058	MsG0180003531.01.T01:CDS	30.0%
ACAAGTCTTCAATCTCAATT+TGG	-	Chr1:63806139-63806158	MsG0180003531.01.T01:intron	30.0%
ACATTGCTAAAAGTTACCAA+GGG	-	Chr1:63806290-63806309	MsG0180003531.01.T01:intron	30.0%
ACCAAAATTAACCTAAAACC+AGG	+	Chr1:63805692-63805711	None:intergenic	30.0%
AGAGATCTTTATCAGAAATC+GGG	-	Chr1:63809102-63809121	MsG0180003531.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	63803190	63809659	63803190	ID=MsG0180003531.01;Name=MsG0180003531.01
Chr1	mRNA	63803190	63809659	63803190	ID=MsG0180003531.01.T01;Parent=MsG0180003531.01;Name=MsG0180003531.01.T01;_AED=0.41;_eAED=0.42;_QI=220\|0.71\|0.87\|1\|0.71\|0.62\|8\|418\|536
Chr1	exon	63803190	63803895	63803190	ID=MsG0180003531.01.T01:exon:23698;Parent=MsG0180003531.01.T01
Chr1	exon	63805032	63805232	63805032	ID=MsG0180003531.01.T01:exon:23697;Parent=MsG0180003531.01.T01
Chr1	exon	63806507	63806737	63806507	ID=MsG0180003531.01.T01:exon:23696;Parent=MsG0180003531.01.T01
Chr1	exon	63806814	63807102	63806814	ID=MsG0180003531.01.T01:exon:23695;Parent=MsG0180003531.01.T01
Chr1	exon	63807208	63807372	63807208	ID=MsG0180003531.01.T01:exon:23694;Parent=MsG0180003531.01.T01
Chr1	exon	63807514	63807638	63807514	ID=MsG0180003531.01.T01:exon:23693;Parent=MsG0180003531.01.T01
Chr1	exon	63808107	63808324	63808107	ID=MsG0180003531.01.T01:exon:23692;Parent=MsG0180003531.01.T01
Chr1	exon	63809346	63809659	63809346	ID=MsG0180003531.01.T01:exon:23691;Parent=MsG0180003531.01.T01
Chr1	five_prime_UTR	63809440	63809659	63809440	ID=MsG0180003531.01.T01:five_prime_utr;Parent=MsG0180003531.01.T01
Chr1	CDS	63809346	63809439	63809346	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63808107	63808324	63808107	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63807514	63807638	63807514	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63807208	63807372	63807208	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63806814	63807102	63806814	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63806507	63806737	63806507	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63805032	63805232	63805032	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	CDS	63803608	63803895	63803608	ID=MsG0180003531.01.T01:cds;Parent=MsG0180003531.01.T01
Chr1	three_prime_UTR	63803190	63803607	63803190	ID=MsG0180003531.01.T01:three_prime_utr;Parent=MsG0180003531.01.T01

Gene Sequence

>MsG0180003531.01.T01

ATGGAATCTGCTAGGACTATGTTTTGTTCTTCTCCACTTTGTCTTCCAAGAACTCACCACTGGAACACTCTTCCTTCATCTTCTTATACTTCTGGTCTTCTGTTATTTGAATCTCGTTTTAATAACCATGGTTACGATGCTTATGTTCATAAACCTGCAGTTTTGATGCTCCGGGAACAAGTTGGGCCTACAGTCACCGCCTGGTGTACTAGTTATTCATCTCAGAACTTTCCTACTTCAGTTCTTTTACAAGAGCAGTGTGATGAGTATAGGCCTCTATTGCATATTTCTAAGAAAGACAAGACATGTCAGGCAACGTTAAATACAACGCAGATGGATAGGGTCTCAGTTCAAGAAGAGAATGACACCAGCAACACTGATCAGGTAGCACGTGACTTCAGGCAGCACCTGCATCTCTTGCCTCGTCTGGAGAACTTATTAACTTCCTCACAAACAGGAGAAATGGATGGTCCTTCAACTTTACAGCATGCAGATAGTTTGCAGTGTAATGCAGTTTCTCCCTCAAAACAAGCAGCCTCGGTAGCTGAAGACTTAATATCAATTAAAGCTGACGAGGATGCTGACAATTCAATTCCTTTTGGTCTCGCCTCTTCCAGTTCGGCTGAACCTTTACTTGGAGGCAACAAAACTGTAAGGTCAACACGACTTCGAGAGAGACGAGCAAAAGAGAGAAAGGTTCCGAAGTCAAAAGTTAAAGACAATGAAACTTACCTTGCTGGAAAAGATGTTCCACAAGAAAAGTTACAAGTACAAAAGAAGATAAATAAAGGAATGAATCAAAACGATCCCTTGAACAAGTTCCTGCAGAGTTCTGATTCGAAGCAACTTTTGACTTCTGAACAAGAGTCGCAGTTGGTTGTTCAGATACAGGAATTATTGAGATTGGAAGAATTGAAGACAAGTCTTCAATCTCAATTTGGAAGGGAACCGACCATGGCTGAATGGGCTGAGGGTGCAGGGCTTAACTGTCGGAAAATGAAGGCGCAACTTCGTTGTGGTAACAGAAGCAGAGAAAAATTGATTCAAGCAAATTTACGCTTGGTACACTACATTGCTAAAAGTTACCAAGGGCGTGGTCTCAGCATTGAGGACTTATTACAGGAGAAGGTATATAACAAACTTAGCAAGGTATTGGAGGCAAAGAAATTGTACATGGAAGAGGGAAATCTTAACCCAACCAAAGAAGAATTAGCAAGAAGGGTAGGAATTTCACCAGACAAGGTTGAGAGGTTACTGTTTGTTGCAAGAATTCCAATTTCTATGCAGAACACTGTGGGGTCTGAGACAGATACAACTTTCCAGGAGATTACTGCAGACAGTGCAATCGAGAGCCCAAACATGAGTGTTGCAAAACAGCTTATGAGACGACATGTGCTCAACGTCTTAAATATCTTACGCCCTAAAGAAAGAAGGATAATTCGGCTAAGATTTGGTTTTGAAGATGGAGAAGAGAGATCTTTATCAGAAATCGGGGAAATATTTGGTTTATCTAGAGAAAGGGTCCGTCAGTTAGAGAGCCGGGCACTCTACAAGCTCAAGAAGTGTCTTGTTGGTCAAGGACTTGATGCCTATGCAGACTTGCTTATATAG

Protein sequence

>MsG0180003531.01.T01

MESARTMFCSSPLCLPRTHHWNTLPSSSYTSGLLLFESRFNNHGYDAYVHKPAVLMLREQVGPTVTAWCTSYSSQNFPTSVLLQEQCDEYRPLLHISKKDKTCQATLNTTQMDRVSVQEENDTSNTDQVARDFRQHLHLLPRLENLLTSSQTGEMDGPSTLQHADSLQCNAVSPSKQAASVAEDLISIKADEDADNSIPFGLASSSSAEPLLGGNKTVRSTRLRERRAKERKVPKSKVKDNETYLAGKDVPQEKLQVQKKINKGMNQNDPLNKFLQSSDSKQLLTSEQESQLVVQIQELLRLEELKTSLQSQFGREPTMAEWAEGAGLNCRKMKAQLRCGNRSREKLIQANLRLVHYIAKSYQGRGLSIEDLLQEKVYNKLSKVLEAKKLYMEEGNLNPTKEELARRVGISPDKVERLLFVARIPISMQNTVGSETDTTFQEITADSAIESPNMSVAKQLMRRHVLNVLNILRPKERRIIRLRFGFEDGEERSLSEIGEIFGLSRERVRQLESRALYKLKKCLVGQGLDAYADLLI*