AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040331.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040331.01.T01 MTR_7g088520 83.333 144 21 1 1 144 1 141 5.53e-77 225
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGGGTTGTGGAGGGAAT+TGG 0.246105 7:+76023359 None:intergenic
TGTGGAGGGAATTGGGAAAA+TGG 0.293091 7:+76023367 None:intergenic
TTGGGGTTGTGGAGGGAATT+GGG 0.318138 7:+76023360 None:intergenic
TTCTGTAGATAAGCTTCCTT+TGG 0.349663 7:-76023052 MsG0780040331.01.T01:CDS
AGTTGAGGAAGAAGGGTAAT+TGG 0.362329 7:-76022936 MsG0780040331.01.T01:CDS
TTCAGACCCATCTGCAACAT+TGG 0.400037 7:+76023104 None:intergenic
AAGAGGAAGTTGAGGAAGAA+GGG 0.402527 7:-76022943 MsG0780040331.01.T01:CDS
TTCCTTGGTTTCACAACAAA+GGG 0.423081 7:+76023162 None:intergenic
AATGTATTGTGGAGAAATTA+AGG 0.435199 7:+76023315 None:intergenic
TCCTTTGGAATCAAAATTGA+AGG 0.438799 7:-76023037 MsG0780040331.01.T01:CDS
AGAAGTGATGGTACTTACTT+GGG 0.448698 7:+76023342 None:intergenic
AAAGAGGAAGTTGAGGAAGA+AGG 0.452118 7:-76022944 MsG0780040331.01.T01:CDS
AAGAAGTGATGGTACTTACT+TGG 0.454332 7:+76023341 None:intergenic
ATGGTACTTACTTGGGGTTG+TGG 0.470559 7:+76023349 None:intergenic
AAATGGAGGAAGAAGAAAGA+GGG 0.478465 7:+76023384 None:intergenic
GAAAGAGACTTGTTCAAAAG+AGG 0.482878 7:-76022960 MsG0780040331.01.T01:CDS
TTGATAAGAAGATAAGGTTG+AGG 0.486048 7:-76022984 MsG0780040331.01.T01:CDS
TGGAATCAAAATTGAAGGAG+AGG 0.491503 7:-76023032 MsG0780040331.01.T01:CDS
TGAAGCTTGATAAGAAGATA+AGG 0.493499 7:-76022990 MsG0780040331.01.T01:CDS
AGAAAGAGGGGTGAGTGTGA+TGG 0.496412 7:+76023397 None:intergenic
GGAATCAAAATTGAAGGAGA+GGG 0.508757 7:-76023031 MsG0780040331.01.T01:CDS
AATTAAGGAAGAAGAAGTGA+TGG 0.531874 7:+76023330 None:intergenic
GTACTTACTTGGGGTTGTGG+AGG 0.532059 7:+76023352 None:intergenic
TTTCCTTGGTTTCACAACAA+AGG 0.537640 7:+76023161 None:intergenic
TACTTACTTGGGGTTGTGGA+GGG 0.541802 7:+76023353 None:intergenic
TGAAGGAGAGGGAAGAGCAG+AGG 0.551586 7:-76023020 MsG0780040331.01.T01:CDS
ATTGAAGGGACTTCGAATCT+CGG 0.553791 7:-76023187 MsG0780040331.01.T01:intron
AAAATGGAGGAAGAAGAAAG+AGG 0.565844 7:+76023383 None:intergenic
TTGTTCAAAAGAGGAAGTTG+AGG 0.577274 7:-76022951 MsG0780040331.01.T01:CDS
AATGGAGGAAGAAGAAAGAG+GGG 0.583049 7:+76023385 None:intergenic
CACCCTTTGTTGTGAAACCA+AGG 0.585823 7:-76023164 MsG0780040331.01.T01:CDS
GCTGAATCCAATGTTGCAGA+TGG 0.602882 7:-76023111 MsG0780040331.01.T01:CDS
GGAGGGAATTGGGAAAATGG+AGG 0.607476 7:+76023370 None:intergenic
CTGAATCCAATGTTGCAGAT+GGG 0.633715 7:-76023110 MsG0780040331.01.T01:CDS
GAAGTGATGGTACTTACTTG+GGG 0.673222 7:+76023343 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAGAATAATAAAAACAT+GGG + Chr7:76023030-76023049 None:intergenic 15.0%
!! TAACAAGAATAATAAAAACA+TGG + Chr7:76023031-76023050 None:intergenic 15.0%
!!! ATAAAAATTGCATTTTTCCT+TGG + Chr7:76023175-76023194 None:intergenic 20.0%
! AATGTATTGTGGAGAAATTA+AGG + Chr7:76023007-76023026 None:intergenic 25.0%
!!! ACTCTTCAATTTTTTCATCT+TGG + Chr7:76023416-76023435 None:intergenic 25.0%
!!! TTCAATTTTTTCATCTTGGA+AGG + Chr7:76023412-76023431 None:intergenic 25.0%
AAAAACATGGGAATGTATTG+TGG + Chr7:76023018-76023037 None:intergenic 30.0%
AATTAAGGAAGAAGAAGTGA+TGG + Chr7:76022992-76023011 None:intergenic 30.0%
TGAAGCTTGATAAGAAGATA+AGG - Chr7:76023329-76023348 MsG0780040331.01.T01:CDS 30.0%
TTGATAAGAAGATAAGGTTG+AGG - Chr7:76023335-76023354 MsG0780040331.01.T01:CDS 30.0%
!! TCCTTTGGAATCAAAATTGA+AGG - Chr7:76023282-76023301 MsG0780040331.01.T01:intron 30.0%
!!! AATTTTTTCATCTTGGAAGG+TGG + Chr7:76023409-76023428 None:intergenic 30.0%
!!! TCCTTCAATTTTGATTCCAA+AGG + Chr7:76023286-76023305 None:intergenic 30.0%
AAAATGGAGGAAGAAGAAAG+AGG + Chr7:76022939-76022958 None:intergenic 35.0%
AAATGGAGGAAGAAGAAAGA+GGG + Chr7:76022938-76022957 None:intergenic 35.0%
AAGAAGTGATGGTACTTACT+TGG + Chr7:76022981-76023000 None:intergenic 35.0%
AGAAGTGATGGTACTTACTT+GGG + Chr7:76022980-76022999 None:intergenic 35.0%
GGAATCAAAATTGAAGGAGA+GGG - Chr7:76023288-76023307 MsG0780040331.01.T01:intron 35.0%
TGGAATCAAAATTGAAGGAG+AGG - Chr7:76023287-76023306 MsG0780040331.01.T01:intron 35.0%
TTCCTTGGTTTCACAACAAA+GGG + Chr7:76023160-76023179 None:intergenic 35.0%
TTCTGTAGATAAGCTTCCTT+TGG - Chr7:76023267-76023286 MsG0780040331.01.T01:intron 35.0%
TTGTTCAAAAGAGGAAGTTG+AGG - Chr7:76023368-76023387 MsG0780040331.01.T01:CDS 35.0%
TTTCCTTGGTTTCACAACAA+AGG + Chr7:76023161-76023180 None:intergenic 35.0%
! GAAAGAGACTTGTTCAAAAG+AGG - Chr7:76023359-76023378 MsG0780040331.01.T01:CDS 35.0%
AAAGAGGAAGTTGAGGAAGA+AGG - Chr7:76023375-76023394 MsG0780040331.01.T01:CDS 40.0%
AAGAGGAAGTTGAGGAAGAA+GGG - Chr7:76023376-76023395 MsG0780040331.01.T01:CDS 40.0%
AAGTCCCTTCAATGAGTTTG+TGG + Chr7:76023125-76023144 None:intergenic 40.0%
AATGGAGGAAGAAGAAAGAG+GGG + Chr7:76022937-76022956 None:intergenic 40.0%
AGTCCCTTCAATGAGTTTGT+GGG + Chr7:76023124-76023143 None:intergenic 40.0%
AGTTGAGGAAGAAGGGTAAT+TGG - Chr7:76023383-76023402 MsG0780040331.01.T01:CDS 40.0%
ATTGAAGGGACTTCGAATCT+CGG - Chr7:76023132-76023151 MsG0780040331.01.T01:CDS 40.0%
CTGAATCCAATGTTGCAGAT+GGG - Chr7:76023209-76023228 MsG0780040331.01.T01:intron 40.0%
GAAGTGATGGTACTTACTTG+GGG + Chr7:76022979-76022998 None:intergenic 40.0%
TTGCCCACAAACTCATTGAA+GGG - Chr7:76023118-76023137 MsG0780040331.01.T01:CDS 40.0%
TTTGCCCACAAACTCATTGA+AGG - Chr7:76023117-76023136 MsG0780040331.01.T01:CDS 40.0%
ATGGTACTTACTTGGGGTTG+TGG + Chr7:76022973-76022992 None:intergenic 45.0%
GCTGAATCCAATGTTGCAGA+TGG - Chr7:76023208-76023227 MsG0780040331.01.T01:intron 45.0%
TACTTACTTGGGGTTGTGGA+GGG + Chr7:76022969-76022988 None:intergenic 45.0%
TGTGGAGGGAATTGGGAAAA+TGG + Chr7:76022955-76022974 None:intergenic 45.0%
TTCAGACCCATCTGCAACAT+TGG + Chr7:76023218-76023237 None:intergenic 45.0%
! CACCCTTTGTTGTGAAACCA+AGG - Chr7:76023155-76023174 MsG0780040331.01.T01:CDS 45.0%
! TCAGTTTCAAGAGTGCACGT+TGG - Chr7:76023094-76023113 MsG0780040331.01.T01:CDS 45.0%
AGAAAGAGGGGTGAGTGTGA+TGG + Chr7:76022925-76022944 None:intergenic 50.0%
GGAGGGAATTGGGAAAATGG+AGG + Chr7:76022952-76022971 None:intergenic 50.0%
GTACTTACTTGGGGTTGTGG+AGG + Chr7:76022970-76022989 None:intergenic 50.0%
TTGGGGTTGTGGAGGGAATT+GGG + Chr7:76022962-76022981 None:intergenic 50.0%
CTTGGGGTTGTGGAGGGAAT+TGG + Chr7:76022963-76022982 None:intergenic 55.0%
TGAAGGAGAGGGAAGAGCAG+AGG - Chr7:76023299-76023318 MsG0780040331.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 76022900 76023441 76022900 ID=MsG0780040331.01;Name=MsG0780040331.01
Chr7 mRNA 76022900 76023441 76022900 ID=MsG0780040331.01.T01;Parent=MsG0780040331.01;Name=MsG0780040331.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|0|0|2|0|144
Chr7 exon 76023310 76023441 76023310 ID=MsG0780040331.01.T01:exon:14914;Parent=MsG0780040331.01.T01
Chr7 exon 76022900 76023202 76022900 ID=MsG0780040331.01.T01:exon:14913;Parent=MsG0780040331.01.T01
Chr7 CDS 76023310 76023441 76023310 ID=MsG0780040331.01.T01:cds;Parent=MsG0780040331.01.T01
Chr7 CDS 76022900 76023202 76022900 ID=MsG0780040331.01.T01:cds;Parent=MsG0780040331.01.T01
Gene Sequence

>MsG0780040331.01.T01

ATGGCACTTCTCTGCTTCAATTCCATCACACTCACCCCTCTTTCTTCTTCCTCCATTTTCCCAATTCCCTCCACAACCCCAAGTAAGTACCATCACTTCTTCTTCCTTAATTTCTCCACAATACATTCCCATGGACTTCGAATCTCGGCACCCTTTGTTGTGAAACCAAGGAAAAATGCAATTTTTATTGCATCTGCTACTGCTGAATCCAATGTTGCAGATGGGTCTGAAAGTGAAAGCAAGAAAGAGAGTGATGCTGTTTCTGTAGATAAGCTTCCTTTGGAATCAAAATTGAAGGAGAGGGAAGAGCAGAGGTTGAAGATGAAGCTTGATAAGAAGATAAGGTTGAGGAGAAAGAGACTTGTTCAAAAGAGGAAGTTGAGGAAGAAGGGTAATTGGCCACCTTCCAAGATGAAAAAATTGAAGAGTGTTTGA

Protein sequence

>MsG0780040331.01.T01

MALLCFNSITLTPLSSSSIFPIPSTTPSKYHHFFFLNFSTIHSHGLRISAPFVVKPRKNAIFIASATAESNVADGSESESKKESDAVSVDKLPLESKLKEREEQRLKMKLDKKIRLRRKRLVQKRKLRKKGNWPPSKMKKLKSV*