AlfalfaGEDB | MsG0480019956.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019956.01

KEGG: K13427
GO: GO:0005525
PFAM: PF01926

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019956.01.T01	GAU27613.1	89.474	323	34	0	5	327	241	563	0	610

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019956.01.T01	Q66GP9	65.357	280	91	2	50	327	286	561	5.47E-129	382

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019956.01.T01	A0A2Z6MBZ2	89.474	323	34	0	5	327	241	563	0.0	610

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0480019956.01	0.837739	4.350104e-57	5.704191e-54
MsG0080047945.01	MsG0480019956.01	0.826619	2.455944e-54	2.315107e-51
MsG0080047955.01	MsG0480019956.01	0.838316	3.091940e-57	4.125497e-54
MsG0080047956.01	MsG0480019956.01	0.820229	7.660589e-53	6.026215e-50
MsG0080047989.01	MsG0480019956.01	0.807147	5.826090e-50	3.220490e-47
MsG0080048068.01	MsG0480019956.01	0.817168	3.793174e-52	2.742021e-49
MsG0080048198.01	MsG0480019956.01	0.806563	7.740988e-50	4.215054e-47
MsG0080048528.01	MsG0480019956.01	0.855385	6.536429e-62	1.506580e-58
MsG0080048727.01	MsG0480019956.01	0.864244	1.398326e-64	4.360703e-61
MsG0080048773.01	MsG0480019956.01	0.861752	8.223856e-64	2.351330e-60

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019956.01.T01	MTR_2g060420	98.201	278	5	0	50	327	270	547	0.0	570
MsG0480019956.01.T01	MTR_2g060420	97.015	134	4	0	50	183	270	403	1.24e-85	265
MsG0480019956.01.T01	MTR_2g060420	98.462	130	2	0	50	179	270	399	1.33e-85	263
MsG0480019956.01.T01	MTR_2g060420	98.925	93	1	0	50	142	270	362	6.11e-58	191
MsG0480019956.01.T01	MTR_7g090640	26.070	257	147	6	50	304	368	583	1.24e-16	81.3
MsG0480019956.01.T01	MTR_4g074870	25.926	216	126	6	50	260	321	507	4.87e-12	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019956.01.T01	AT3G47450	65.357	280	91	2	50	327	286	561	5.58e-130	382
MsG0480019956.01.T01	AT3G47450	64.643	280	93	2	50	327	286	561	1.50e-128	379
MsG0480019956.01.T01	AT4G10620	25.292	257	149	6	45	299	315	530	1.18e-15	78.2

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Find 87 sgRNAs with CRISPR-Local

Find 367 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAATAGACACTACG+GGG	0.732571	4:-30655389	MsG0480019956.01.T01:CDS
AAAGGTGCTTCGACAGTAAA+TGG	0.298977	4:-30656814	MsG0480019956.01.T01:CDS
AAATCATTGGTAGGAATAAC+TGG	0.335779	4:-30660270	MsG0480019956.01.T01:CDS
AAATGCAATAGACACTACGG+GGG	0.756248	4:-30655388	MsG0480019956.01.T01:CDS
AAATTCATCTGCTTTCTCTG+TGG	0.589837	4:+30656566	None:intergenic
AACAAACCTTCTTCTCTTTC+TGG	0.093378	4:+30660232	None:intergenic
AACAGGTTTGGGGACATGTA+CGG	0.568281	4:+30655353	None:intergenic
AACATTGGTTGTTGAGAACT+TGG	0.383313	4:+30655128	None:intergenic
AAGAAAGCATTAATTTGAAT+TGG	0.326794	4:+30657164	None:intergenic
AAGAACATACCGGGAAAGAT+AGG	0.460823	4:+30656944	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCATTGGTAGGAATAAC+TGG	-	Chr4:30655080-30655099	MsG0480019956.01.T01:three_prime_UTR	30.0%
AATATGGATTTGGATGGAAA+TGG	+	Chr4:30659737-30659756	None:intergenic	30.0%
AATGAACTTGTCAAATGTCA+AGG	-	Chr4:30655373-30655392	MsG0480019956.01.T01:CDS	30.0%
AATGCAAACAATGACTACAA+CGG	+	Chr4:30656510-30656529	None:intergenic	30.0%
ACAAAATGCAATAGACACTA+CGG	-	Chr4:30659959-30659978	MsG0480019956.01.T01:intron	30.0%
ACACCTTCATAAAAAGTAGT+TGG	-	Chr4:30657634-30657653	MsG0480019956.01.T01:intron	30.0%
ACAGATGAATCTTCCATAAA+TGG	+	Chr4:30656112-30656131	None:intergenic	30.0%
AGAAATTTAGGGGATTGTAA+TGG	-	Chr4:30659688-30659707	MsG0480019956.01.T01:intron	30.0%
AGAATTAACAGATAAAGAGC+AGG	-	Chr4:30660082-30660101	MsG0480019956.01.T01:intron	30.0%
AGTTTGTACTTTCATGTCAT+AGG	-	Chr4:30658636-30658655	MsG0480019956.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	30655046	30660326	30655046	ID=MsG0480019956.01;Name=MsG0480019956.01
Chr4	mRNA	30655046	30660326	30655046	ID=MsG0480019956.01.T01;Parent=MsG0480019956.01;Name=MsG0480019956.01.T01;_AED=0.44;_eAED=0.46;_QI=0\|0.87\|0.77\|1\|0.62\|0.66\|9\|196\|327
Chr4	exon	30655046	30655491	30655046	ID=MsG0480019956.01.T01:exon:10017;Parent=MsG0480019956.01.T01
Chr4	exon	30655943	30656052	30655943	ID=MsG0480019956.01.T01:exon:10016;Parent=MsG0480019956.01.T01
Chr4	exon	30656560	30656649	30656560	ID=MsG0480019956.01.T01:exon:10015;Parent=MsG0480019956.01.T01
Chr4	exon	30656759	30656866	30656759	ID=MsG0480019956.01.T01:exon:10014;Parent=MsG0480019956.01.T01
Chr4	exon	30656954	30657067	30656954	ID=MsG0480019956.01.T01:exon:10013;Parent=MsG0480019956.01.T01
Chr4	exon	30657154	30657272	30657154	ID=MsG0480019956.01.T01:exon:10012;Parent=MsG0480019956.01.T01
Chr4	exon	30658994	30659039	30658994	ID=MsG0480019956.01.T01:exon:10011;Parent=MsG0480019956.01.T01
Chr4	exon	30659593	30659651	30659593	ID=MsG0480019956.01.T01:exon:10010;Parent=MsG0480019956.01.T01
Chr4	exon	30660239	30660326	30660239	ID=MsG0480019956.01.T01:exon:10009;Parent=MsG0480019956.01.T01
Chr4	CDS	30660239	30660326	30660239	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30659593	30659651	30659593	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30658994	30659039	30658994	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30657154	30657272	30657154	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30656954	30657067	30656954	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30656759	30656866	30656759	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30656560	30656649	30656560	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30655943	30656052	30655943	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	CDS	30655242	30655491	30655242	ID=MsG0480019956.01.T01:cds;Parent=MsG0480019956.01.T01
Chr4	three_prime_UTR	30655046	30655241	30655046	ID=MsG0480019956.01.T01:three_prime_utr;Parent=MsG0480019956.01.T01

Gene Sequence

>MsG0480019956.01.T01

TTGCAGTGTTCTCAGTGTCCATCTTACGAGTTCAAAATCATTGGTAGGAATAACTGGAGTGATATCAGAAATCCAGAAAGAGAAGAAGGGAAGAGATGTTTACATTCTGGTAAGTCTCTGAACTGGAAGATTCATATGCTTAAGCAGGGTTCAGCTAATGTTGGTAAATCTGCTTTCATTAACGCCTTATTAAAGACAATGGCATATAATGATCCAGTGGCTGCAGCTGCACAAAGATACAAACCAGTACAGTCTGCTGTTCCTGGAACTACCTTAGGGCCAATTCAAATTAATGCTTTCTTTGGAGGAGGGAAACTGTATGACACTCCAGGAGTTCATCTCCACCACAGGCAAACTGCAGTTGTTCCTTCCGAAGATCTATCCTCCCTTGCTCCTAAAAGCCGACTGAGGGGCCTATCTTTCCCGAGTTCACAAGTACTTTCCGACAATACAAACAAAGGTGCTTCGACAGTAAATGGCTTGAATGGATTTTCAATATTTTGGGGAGGTCTTGTTAGAATTGATGTCTTGAAGGCTCTACCGGAAACATGTTTAACATTTTACGGGCCTAAGAGGATGCCAATTCATATGGTGCCCACAGAGAAAGCAGATGAATTTTATCAGAAAGAACTTGGAGTTCTGCTAATCCCACCAAGTGGAAAAGAGAAGGCTGAGAACTGGAGAGGACTTGACTCAGAACGTAAATTGCAAATAAAATTTGAAGATACTGAAAGGCCAGCTTGCGATATTGCTATATCAGGTCTAGGATGGCTTTCTGTTGAGCCGGTTGGTAGGTCACACAGATTCTCACAACAAAATGCAATAGACACTACGGGGGAATTGCTTTTAGCCGTACATGTCCCCAAACCTGTTGAGATTTTTACGAGGCCACCATTACCAGTAGGCAAGGCTGGGGCAGAATGGTACGAGTATGCAGAATTAACAGATAAAGAGCAGGAAATGAGACCAAAATGGTACTTTTGA

Protein sequence

>MsG0480019956.01.T01

LQCSQCPSYEFKIIGRNNWSDIRNPEREEGKRCLHSGKSLNWKIHMLKQGSANVGKSAFINALLKTMAYNDPVAAAAQRYKPVQSAVPGTTLGPIQINAFFGGGKLYDTPGVHLHHRQTAVVPSEDLSSLAPKSRLRGLSFPSSQVLSDNTNKGASTVNGLNGFSIFWGGLVRIDVLKALPETCLTFYGPKRMPIHMVPTEKADEFYQKELGVLLIPPSGKEKAENWRGLDSERKLQIKFEDTERPACDIAISGLGWLSVEPVGRSHRFSQQNAIDTTGELLLAVHVPKPVEIFTRPPLPVGKAGAEWYEYAELTDKEQEMRPKWYF*