AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019751.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019751.01.T01 MTR_4g037505 94.175 103 5 1 1 102 6 108 1.28e-60 181
MsG0480019751.01.T01 MTR_3g030850 66.667 102 31 3 1 101 6 105 2.31e-37 122
MsG0480019751.01.T01 MTR_3g030860 68.750 96 27 3 8 102 12 105 7.19e-30 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019751.01.T01 AT3G21055 50.476 105 42 4 1 102 6 103 8.66e-20 77.4
MsG0480019751.01.T01 AT3G21055 50.476 105 42 4 1 102 46 143 1.79e-19 77.8
MsG0480019751.01.T01 AT1G51400 46.154 104 49 3 1 101 6 105 1.73e-18 74.3

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTTGTTGGAATGGCTT+TGG 0.310934 4:-26368817 MsG0480019751.01.T01:CDS
GCTTCAGGTGAACCACGTTT+TGG 0.335997 4:+26368786 None:intergenic
GCATAGGCTTTCTTAGCTTC+AGG 0.364633 4:+26368771 None:intergenic
GCAGCTGTTTGTTCTGTTGT+TGG 0.390026 4:-26368828 MsG0480019751.01.T01:CDS
TTCAACTGCTTTGGCAGCAT+TGG 0.404500 4:+26368932 None:intergenic
GGTAGCAACTCAGAGGAAAC+TGG 0.430961 4:-26368961 MsG0480019751.01.T01:CDS
TGTTTGTTCTGTTGTTGGAA+TGG 0.441127 4:-26368823 MsG0480019751.01.T01:CDS
GTTGGCATGGTGACACAAAC+AGG 0.454036 4:+26368747 None:intergenic
GAAGCTCAGCCATCGTCAAC+CGG 0.458400 4:-26368989 MsG0480019751.01.T01:CDS
ACCATGACAGCAGCATTCTT+CGG 0.487676 4:-26369014 MsG0480019751.01.T01:CDS
CATGATGTTCCTCCTTCCAT+TGG 0.502517 4:+26368866 None:intergenic
GGTGAGTTATGACAATGAAA+AGG 0.503070 4:-26368895 MsG0480019751.01.T01:CDS
TCTGAGTTGCTACCGGAGAC+CGG 0.503643 4:+26368970 None:intergenic
AGCCATCGTCAACCGGTCTC+CGG 0.513044 4:-26368982 MsG0480019751.01.T01:CDS
AGTTTCCTCTGAGTTGCTAC+CGG 0.542047 4:+26368963 None:intergenic
GTGTCACCATGCCAACAGCT+AGG 0.561750 4:-26368740 MsG0480019751.01.T01:CDS
GCCGAAGAATGCTGCTGTCA+TGG 0.580952 4:+26369013 None:intergenic
TAGCGACAAATCCTAGCTGT+TGG 0.592341 4:+26368729 None:intergenic
GGTGACACAAACAGGAGCAT+AGG 0.599674 4:+26368755 None:intergenic
AATGAAAAGGAAGAGACCAA+TGG 0.609364 4:-26368882 MsG0480019751.01.T01:CDS
ACAAATCCTAGCTGTTGGCA+TGG 0.619696 4:+26368734 None:intergenic
AGGAAGAGACCAATGGAAGG+AGG 0.639763 4:-26368875 MsG0480019751.01.T01:CDS
TACCGGAGACCGGTTGACGA+TGG 0.647391 4:+26368980 None:intergenic
AAAAGGAAGAGACCAATGGA+AGG 0.647549 4:-26368878 MsG0480019751.01.T01:CDS
TTGGCTGATGAGCCAAAACG+TGG 0.652108 4:-26368798 MsG0480019751.01.T01:CDS
ATGACAGCAGCATTCTTCGG+CGG 0.688662 4:-26369011 MsG0480019751.01.T01:CDS
GGTCTCCGGTAGCAACTCAG+AGG 0.693887 4:-26368968 MsG0480019751.01.T01:CDS
AGCAACTCAGAGGAAACTGG+TGG 0.716307 4:-26368958 MsG0480019751.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTTGAAGTAGAAAAAATGA+AGG - Chr4:26368820-26368839 MsG0480019751.01.T01:CDS 25.0%
! TTTTTCTACTTCAACTGCTT+TGG + Chr4:26368816-26368835 None:intergenic 30.0%
AATGAAAAGGAAGAGACCAA+TGG - Chr4:26368854-26368873 MsG0480019751.01.T01:CDS 35.0%
GGTGAGTTATGACAATGAAA+AGG - Chr4:26368841-26368860 MsG0480019751.01.T01:CDS 35.0%
TGTTTGTTCTGTTGTTGGAA+TGG - Chr4:26368913-26368932 MsG0480019751.01.T01:CDS 35.0%
AAAAGGAAGAGACCAATGGA+AGG - Chr4:26368858-26368877 MsG0480019751.01.T01:CDS 40.0%
TTCTGTTGTTGGAATGGCTT+TGG - Chr4:26368919-26368938 MsG0480019751.01.T01:CDS 40.0%
ACAAATCCTAGCTGTTGGCA+TGG + Chr4:26369005-26369024 None:intergenic 45.0%
AGTTTCCTCTGAGTTGCTAC+CGG + Chr4:26368776-26368795 None:intergenic 45.0%
CATGATGTTCCTCCTTCCAT+TGG + Chr4:26368873-26368892 None:intergenic 45.0%
GCAGCTGTTTGTTCTGTTGT+TGG - Chr4:26368908-26368927 MsG0480019751.01.T01:CDS 45.0%
GCATAGGCTTTCTTAGCTTC+AGG + Chr4:26368968-26368987 None:intergenic 45.0%
TAGCGACAAATCCTAGCTGT+TGG + Chr4:26369010-26369029 None:intergenic 45.0%
TTCAACTGCTTTGGCAGCAT+TGG + Chr4:26368807-26368826 None:intergenic 45.0%
AGCAACTCAGAGGAAACTGG+TGG - Chr4:26368778-26368797 MsG0480019751.01.T01:CDS 50.0%
AGGAAGAGACCAATGGAAGG+AGG - Chr4:26368861-26368880 MsG0480019751.01.T01:CDS 50.0%
ATGACAGCAGCATTCTTCGG+CGG - Chr4:26368725-26368744 MsG0480019751.01.T01:CDS 50.0%
GGTAGCAACTCAGAGGAAAC+TGG - Chr4:26368775-26368794 MsG0480019751.01.T01:CDS 50.0%
GTTGGCATGGTGACACAAAC+AGG + Chr4:26368992-26369011 None:intergenic 50.0%
TTGGCTGATGAGCCAAAACG+TGG - Chr4:26368938-26368957 MsG0480019751.01.T01:CDS 50.0%
! GCTTCAGGTGAACCACGTTT+TGG + Chr4:26368953-26368972 None:intergenic 50.0%
! GGTGACACAAACAGGAGCAT+AGG + Chr4:26368984-26369003 None:intergenic 50.0%
GAAGCTCAGCCATCGTCAAC+CGG - Chr4:26368747-26368766 MsG0480019751.01.T01:CDS 55.0%
GTGTCACCATGCCAACAGCT+AGG - Chr4:26368996-26369015 MsG0480019751.01.T01:CDS 55.0%
TCTGAGTTGCTACCGGAGAC+CGG + Chr4:26368769-26368788 None:intergenic 55.0%
AGCCATCGTCAACCGGTCTC+CGG - Chr4:26368754-26368773 MsG0480019751.01.T01:CDS 60.0%
GGTCTCCGGTAGCAACTCAG+AGG - Chr4:26368768-26368787 MsG0480019751.01.T01:CDS 60.0%
TACCGGAGACCGGTTGACGA+TGG + Chr4:26368759-26368778 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 26368725 26369033 26368725 ID=MsG0480019751.01;Name=MsG0480019751.01
Chr4 mRNA 26368725 26369033 26368725 ID=MsG0480019751.01.T01;Parent=MsG0480019751.01;Name=MsG0480019751.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|102
Chr4 exon 26368725 26369033 26368725 ID=MsG0480019751.01.T01:exon:9100;Parent=MsG0480019751.01.T01
Chr4 CDS 26368725 26369033 26368725 ID=MsG0480019751.01.T01:cds;Parent=MsG0480019751.01.T01
Gene Sequence

>MsG0480019751.01.T01

ATGACAGCAGCATTCTTCGGCGGAAGCTCAGCCATCGTCAACCGGTCTCCGGTAGCAACTCAGAGGAAACTGGTGGTAGCCAATGCTGCCAAAGCAGTTGAAGTAGAAAAAATGAAGGTGAGTTATGACAATGAAAAGGAAGAGACCAATGGAAGGAGGAACATCATGTTTGCTGCTGCAGCAGCAGCTGTTTGTTCTGTTGTTGGAATGGCTTTGGCTGATGAGCCAAAACGTGGTTCACCTGAAGCTAAGAAAGCCTATGCTCCTGTTTGTGTCACCATGCCAACAGCTAGGATTTGTCGCTACTAA

Protein sequence

>MsG0480019751.01.T01

MTAAFFGGSSAIVNRSPVATQRKLVVANAAKAVEVEKMKVSYDNEKEETNGRRNIMFAAAAAAVCSVVGMALADEPKRGSPEAKKAYAPVCVTMPTARICRY*