AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041480.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041480.01.T01 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.44e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041480.01.T01 AT4G15510 64.659 249 76 5 10 247 40 287 4.25e-105 306
MsG0780041480.01.T01 AT4G15510 73.039 204 52 2 46 247 3 205 2.10e-103 298
MsG0780041480.01.T01 AT4G15510 73.039 204 52 2 46 247 3 205 2.10e-103 298
MsG0780041480.01.T01 AT4G15510 73.039 204 52 2 46 247 3 205 2.10e-103 298
MsG0780041480.01.T01 AT4G15510 69.595 148 40 3 35 178 72 218 1.73e-62 196

Find 70 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAACTATCCATCAAAT+TGG 0.259378 7:-91422151 MsG0780041480.01.T01:CDS
GGCTTCGTTTCGAGCAAATA+AGG 0.293344 7:-91421492 MsG0780041480.01.T01:CDS
TGTACAAATGCAGCAAAGTT+TGG 0.296776 7:-91421336 MsG0780041480.01.T01:three_prime_UTR
CTTACTTGAATCCAATTTGA+TGG 0.303876 7:+91422140 None:intergenic
AGGAGGTACCTGTCTGTTCT+AGG 0.353510 7:-91421602 MsG0780041480.01.T01:CDS
GAATATGTTGATACATTTGA+TGG 0.354613 7:-91422179 MsG0780041480.01.T01:CDS
ATGGATGGAATTTACCGAAT+TGG 0.356971 7:+91422395 None:intergenic
ACAAGATAGGGTGGTGCGTT+TGG 0.360551 7:-91421633 MsG0780041480.01.T01:CDS
TGTTATGCTTATAACAAAAC+TGG 0.364964 7:+91421265 None:intergenic
TTGCTGTTATGCCACAAGAT+AGG 0.370954 7:-91421646 MsG0780041480.01.T01:CDS
TGGATGGAATTTACCGAATT+GGG 0.381934 7:+91422396 None:intergenic
CAAAACTGGTCAAGAATAGA+AGG 0.391626 7:+91421279 None:intergenic
CGAATTGGGTGTGGGAGAAA+TGG 0.397956 7:+91422410 None:intergenic
CTAGAATGTTGTAAATCAAA+AGG 0.409063 7:-91421456 MsG0780041480.01.T01:three_prime_UTR
CATTTCTCCCACACCCAATT+CGG 0.411234 7:-91422409 MsG0780041480.01.T01:intron
GCCAATACAATCACAACAAC+GGG 0.413254 7:-91422483 MsG0780041480.01.T01:CDS
TATTCTTGATGAAAATATAT+CGG 0.441156 7:-91422081 MsG0780041480.01.T01:CDS
TCAACTGCCGTTGTTAATGA+TGG 0.449571 7:-91421385 MsG0780041480.01.T01:three_prime_UTR
TAGGGTGGTGCGTTTGGAAT+GGG 0.452062 7:-91421627 MsG0780041480.01.T01:CDS
ATTCAAGACTGTTCAAGACT+TGG 0.455381 7:-91422030 MsG0780041480.01.T01:CDS
GGAATTTACCGAATTGGGTG+TGG 0.465108 7:+91422401 None:intergenic
TTGGGTTCGCCTCAACAAGC+TGG 0.465683 7:-91422011 MsG0780041480.01.T01:CDS
GAATTCACATGATGCTAATA+CGG 0.467654 7:+91421145 None:intergenic
TCAAATTGGATTCAAGTAAG+AGG 0.471228 7:-91422137 MsG0780041480.01.T01:CDS
CGAGGGGAATGGAAGTGAAA+TGG 0.477757 7:+91422438 None:intergenic
ACTTACATTCTCTCCGACAT+TGG 0.478283 7:-91422236 MsG0780041480.01.T01:CDS
TTCAAGACTGTTCAAGACTT+GGG 0.478605 7:-91422029 MsG0780041480.01.T01:CDS
ACTCACACAGCAAAGGCAAA+TGG 0.481060 7:+91422519 None:intergenic
TGGGAGAAATGGAGCAGCGA+GGG 0.485253 7:+91422421 None:intergenic
ACCCGTTGTTGTGATTGTAT+TGG 0.491544 7:+91422482 None:intergenic
AAATGGAGCAGCGAGGGGAA+TGG 0.491691 7:+91422427 None:intergenic
TGCTGTTATGCCACAAGATA+GGG 0.492092 7:-91421645 MsG0780041480.01.T01:CDS
GTGAAATGGTGACATGGGTC+GGG 0.494548 7:+91422452 None:intergenic
GAGTTTATGTCCACTAGGCT+TGG 0.508538 7:-91421960 MsG0780041480.01.T01:CDS
GGTCAAAGATCATATGAATA+CGG 0.509948 7:+91421120 None:intergenic
TGGATTCAAGTAAGAGGTGC+TGG 0.510176 7:-91422131 MsG0780041480.01.T01:CDS
AGAAAATGTGTTTCAAGAAG+AGG 0.524955 7:-91421537 MsG0780041480.01.T01:CDS
TAGAATGTTGTAAATCAAAA+GGG 0.529208 7:-91421455 MsG0780041480.01.T01:three_prime_UTR
TCTGAGAGAGTTGCTGATGA+TGG 0.533806 7:-91421906 MsG0780041480.01.T01:CDS
ATAGGGTGGTGCGTTTGGAA+TGG 0.545277 7:-91421628 MsG0780041480.01.T01:CDS
CTTTGCTGTGTGAGTCGCAT+CGG 0.546482 7:-91422511 MsG0780041480.01.T01:five_prime_UTR
TGGGTTCGCCTCAACAAGCT+GGG 0.554823 7:-91422010 MsG0780041480.01.T01:CDS
TGGTGCGTTTGGAATGGGAT+AGG 0.554855 7:-91421622 MsG0780041480.01.T01:CDS
TTACTGAGTTTATGTCCACT+AGG 0.557448 7:-91421965 MsG0780041480.01.T01:CDS
GTCTTGAATTTCGAAGAAGA+AGG 0.559580 7:+91422044 None:intergenic
GTAATCTCAACTCATACAGT+TGG 0.560478 7:+91421567 None:intergenic
ACTTTCTTCCCAGCTTGTTG+AGG 0.564235 7:+91422002 None:intergenic
AGTGAAATGGTGACATGGGT+CGG 0.571672 7:+91422451 None:intergenic
ATCAGCAACTCTCTCAGAGG+TGG 0.572411 7:+91421911 None:intergenic
GAATTTACCGAATTGGGTGT+GGG 0.577884 7:+91422402 None:intergenic
TTTCAACTCCTAGAACAGAC+AGG 0.577991 7:+91421594 None:intergenic
TGTGTGAGTCGCATCGGCAA+TGG 0.582093 7:-91422505 MsG0780041480.01.T01:exon
ATGGAAGTGAAATGGTGACA+TGG 0.594314 7:+91422446 None:intergenic
GAGTGAACTTCGTCAAGTCA+TGG 0.596736 7:-91421513 MsG0780041480.01.T01:CDS
ACGTTTAACACCAAGCCTAG+TGG 0.600812 7:+91421950 None:intergenic
CAAATTGTACTATCAAGTTG+AGG 0.601868 7:-91421883 MsG0780041480.01.T01:intron
TGCTAATGCTGTACCAATGT+CGG 0.609859 7:+91422223 None:intergenic
GGCCAATACAATCACAACAA+CGG 0.609937 7:-91422484 MsG0780041480.01.T01:CDS
CTTGCAAGATTAATATGCGA+TGG 0.617092 7:-91421241 MsG0780041480.01.T01:three_prime_UTR
GTGGGAGAAATGGAGCAGCG+AGG 0.622596 7:+91422420 None:intergenic
TGCGTTTGGAATGGGATAGG+AGG 0.627210 7:-91421619 MsG0780041480.01.T01:CDS
CAGCAAAGTTTGGATTGAGA+TGG 0.629144 7:-91421326 MsG0780041480.01.T01:three_prime_UTR
AAACGCACCACCCTATCTTG+TGG 0.644066 7:+91421635 None:intergenic
CGATGCGACTCACACAGCAA+AGG 0.646688 7:+91422512 None:intergenic
TGGAAGTGAAATGGTGACAT+GGG 0.658481 7:+91422447 None:intergenic
GGGAGAAATGGAGCAGCGAG+GGG 0.672235 7:+91422422 None:intergenic
ATCATCAGCAACTCTCTCAG+AGG 0.672975 7:+91421908 None:intergenic
ACTGCTACCATCATTAACAA+CGG 0.699682 7:+91421378 None:intergenic
TGTTATGCCACAAGATAGGG+TGG 0.714139 7:-91421642 MsG0780041480.01.T01:CDS
ACATATTCACGAAACACACG+AGG 0.719885 7:+91422194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG - Chr7:91421590-91421609 MsG0780041480.01.T01:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG + Chr7:91421588-91421607 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG + Chr7:91421316-91421335 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG - Chr7:91421817-91421836 MsG0780041480.01.T01:intron 20.0%
!! TAGAATGTTGTAAATCAAAA+GGG - Chr7:91422216-91422235 MsG0780041480.01.T01:CDS 20.0%
! CTAGAATGTTGTAAATCAAA+AGG - Chr7:91422215-91422234 MsG0780041480.01.T01:CDS 25.0%
! GAATATGTTGATACATTTGA+TGG - Chr7:91421492-91421511 MsG0780041480.01.T01:CDS 25.0%
! TGTTATGCTTATAACAAAAC+TGG + Chr7:91422409-91422428 None:intergenic 25.0%
AAAACTTACGGTAATTTGAG+GGG - Chr7:91421865-91421884 MsG0780041480.01.T01:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG - Chr7:91422134-91422153 MsG0780041480.01.T01:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG - Chr7:91421864-91421883 MsG0780041480.01.T01:intron 30.0%
CATTCAACTATCCATCAAAT+TGG - Chr7:91421520-91421539 MsG0780041480.01.T01:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG + Chr7:91421534-91421553 None:intergenic 30.0%
GAATTCACATGATGCTAATA+CGG + Chr7:91422529-91422548 None:intergenic 30.0%
GGTCAAAGATCATATGAATA+CGG + Chr7:91422554-91422573 None:intergenic 30.0%
TAGAAGAAAGAAGAACAATG+CGG + Chr7:91421121-91421140 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG - Chr7:91421534-91421553 MsG0780041480.01.T01:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG - Chr7:91421788-91421807 MsG0780041480.01.T01:intron 30.0%
! TCTTCTTGAAACACATTTTC+TGG + Chr7:91422135-91422154 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG + Chr7:91421405-91421424 None:intergenic 30.0%
ACTGCTACCATCATTAACAA+CGG + Chr7:91422296-91422315 None:intergenic 35.0%
ATGGATGGAATTTACCGAAT+TGG + Chr7:91421279-91421298 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG - Chr7:91421641-91421660 MsG0780041480.01.T01:CDS 35.0%
CAAAACTGGTCAAGAATAGA+AGG + Chr7:91422395-91422414 None:intergenic 35.0%
CTCTGTTCTTGCAAAACTTA+CGG - Chr7:91421853-91421872 MsG0780041480.01.T01:intron 35.0%
CTTGCAAGATTAATATGCGA+TGG - Chr7:91422430-91422449 MsG0780041480.01.T01:CDS 35.0%
GCAAAACTTACGGTAATTTG+AGG - Chr7:91421863-91421882 MsG0780041480.01.T01:intron 35.0%
GTAATCTCAACTCATACAGT+TGG + Chr7:91422107-91422126 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG + Chr7:91421630-91421649 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG + Chr7:91421278-91421297 None:intergenic 35.0%
TGTACAAATGCAGCAAAGTT+TGG - Chr7:91422335-91422354 MsG0780041480.01.T01:intron 35.0%
TTACTGAGTTTATGTCCACT+AGG - Chr7:91421706-91421725 MsG0780041480.01.T01:intron 35.0%
TTCAAGACTGTTCAAGACTT+GGG - Chr7:91421642-91421661 MsG0780041480.01.T01:CDS 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG - Chr7:91421560-91421579 MsG0780041480.01.T01:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG - Chr7:91421879-91421898 MsG0780041480.01.T01:intron 35.0%
ACATATTCACGAAACACACG+AGG + Chr7:91421480-91421499 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG + Chr7:91421192-91421211 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG - Chr7:91421435-91421454 MsG0780041480.01.T01:three_prime_UTR 40.0%
AGAGGAGAAAGCTCATGAAA+TGG + Chr7:91421298-91421317 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG + Chr7:91421228-91421247 None:intergenic 40.0%
CAGCAAAGTTTGGATTGAGA+TGG - Chr7:91422345-91422364 MsG0780041480.01.T01:intron 40.0%
GAATTTACCGAATTGGGTGT+GGG + Chr7:91421272-91421291 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG + Chr7:91421294-91421313 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG - Chr7:91421188-91421207 MsG0780041480.01.T01:three_prime_UTR 40.0%
GGCCAATACAATCACAACAA+CGG - Chr7:91421187-91421206 MsG0780041480.01.T01:three_prime_UTR 40.0%
TCAACTGCCGTTGTTAATGA+TGG - Chr7:91422286-91422305 MsG0780041480.01.T01:CDS 40.0%
TGCTAATGCTGTACCAATGT+CGG + Chr7:91421451-91421470 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG - Chr7:91422026-91422045 MsG0780041480.01.T01:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG + Chr7:91421227-91421246 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG + Chr7:91422080-91422099 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG - Chr7:91422025-91422044 MsG0780041480.01.T01:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG - Chr7:91421969-91421988 MsG0780041480.01.T01:CDS 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG - Chr7:91421559-91421578 MsG0780041480.01.T01:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG + Chr7:91421724-91421743 None:intergenic 45.0%
ACTCACACAGCAAAGGCAAA+TGG + Chr7:91421155-91421174 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG + Chr7:91421223-91421242 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG + Chr7:91421766-91421785 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG - Chr7:91421262-91421281 MsG0780041480.01.T01:three_prime_UTR 45.0%
GAGTGAACTTCGTCAAGTCA+TGG - Chr7:91422158-91422177 MsG0780041480.01.T01:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG - Chr7:91421711-91421730 MsG0780041480.01.T01:intron 45.0%
GGAATTTACCGAATTGGGTG+TGG + Chr7:91421273-91421292 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG - Chr7:91422179-91422198 MsG0780041480.01.T01:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG - Chr7:91421765-91421784 MsG0780041480.01.T01:intron 45.0%
TGTTATGCCACAAGATAGGG+TGG - Chr7:91422029-91422048 MsG0780041480.01.T01:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG - Chr7:91421540-91421559 MsG0780041480.01.T01:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG + Chr7:91421672-91421691 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG + Chr7:91422039-91422058 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG - Chr7:91422038-91422057 MsG0780041480.01.T01:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG - Chr7:91422043-91422062 MsG0780041480.01.T01:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG + Chr7:91421763-91421782 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG + Chr7:91421264-91421283 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG + Chr7:91421236-91421255 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG + Chr7:91421222-91421241 None:intergenic 50.0%
! CTTTGCTGTGTGAGTCGCAT+CGG - Chr7:91421160-91421179 MsG0780041480.01.T01:three_prime_UTR 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG - Chr7:91422044-91422063 MsG0780041480.01.T01:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG - Chr7:91422052-91422071 MsG0780041480.01.T01:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG - Chr7:91422049-91422068 MsG0780041480.01.T01:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG - Chr7:91422069-91422088 MsG0780041480.01.T01:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG + Chr7:91421247-91421266 None:intergenic 55.0%
CGATGCGACTCACACAGCAA+AGG + Chr7:91421162-91421181 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG + Chr7:91421253-91421272 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG - Chr7:91421661-91421680 MsG0780041480.01.T01:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG - Chr7:91421660-91421679 MsG0780041480.01.T01:CDS 55.0%
! TGTGTGAGTCGCATCGGCAA+TGG - Chr7:91421166-91421185 MsG0780041480.01.T01:three_prime_UTR 55.0%
GGGAGAAATGGAGCAGCGAG+GGG + Chr7:91421252-91421271 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG + Chr7:91421254-91421273 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 91421120 91422573 91421120 ID=MsG0780041480.01;Name=MsG0780041480.01
Chr7 mRNA 91421120 91422573 91421120 ID=MsG0780041480.01.T01;Parent=MsG0780041480.01;Name=MsG0780041480.01.T01;_AED=0.23;_eAED=0.23;_QI=65|1|1|1|1|1|3|361|249
Chr7 exon 91421120 91421702 91421120 ID=MsG0780041480.01.T01:exon:18735;Parent=MsG0780041480.01.T01
Chr7 exon 91421884 91422312 91421884 ID=MsG0780041480.01.T01:exon:18734;Parent=MsG0780041480.01.T01
Chr7 exon 91422410 91422573 91422410 ID=MsG0780041480.01.T01:exon:18733;Parent=MsG0780041480.01.T01
Chr7 five_prime_UTR 91422509 91422573 91422509 ID=MsG0780041480.01.T01:five_prime_utr;Parent=MsG0780041480.01.T01
Chr7 CDS 91422410 91422508 91422410 ID=MsG0780041480.01.T01:cds;Parent=MsG0780041480.01.T01
Chr7 CDS 91421884 91422312 91421884 ID=MsG0780041480.01.T01:cds;Parent=MsG0780041480.01.T01
Chr7 CDS 91421481 91421702 91421481 ID=MsG0780041480.01.T01:cds;Parent=MsG0780041480.01.T01
Chr7 three_prime_UTR 91421120 91421480 91421120 ID=MsG0780041480.01.T01:three_prime_utr;Parent=MsG0780041480.01.T01
Gene Sequence

>MsG0780041480.01.T01

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGCGAACATTAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MsG0780041480.01.T01

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR*