AlfalfaGEDB | MsG0280006590.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006590.01

KEGG: -
GO: GO:0004674
PFAM: PF11883

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006590.01.T01	AES63672.2	92.453	53	4	0	1	53	789	841	2.26E-23	102

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006590.01.T01	G7IK62	92.453	53	4	0	1	53	789	841	1.08e-23	102

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000008.01	MsG0280006590.01	0.823471	1.360660e-53	1.172642e-50
MsG0180001663.01	MsG0280006590.01	0.828032	1.126383e-54	1.106459e-51
MsG0180003189.01	MsG0280006590.01	0.805512	1.287804e-49	6.825967e-47
MsG0180006134.01	MsG0280006590.01	0.809288	2.037598e-50	1.191590e-47
MsG0280006325.01	MsG0280006590.01	0.809686	1.673566e-50	9.891321e-48
MsG0280006588.01	MsG0280006590.01	0.826022	3.408031e-54	3.158508e-51
MsG0280006589.01	MsG0280006590.01	0.814036	1.889815e-51	1.254322e-48
MsG0280006590.01	MsG0280006694.01	0.808345	3.241150e-50	1.848782e-47
MsG0280006590.01	MsG0280008112.01	0.805325	1.409947e-49	7.437665e-47
MsG0280006590.01	MsG0280008113.01	0.803298	3.726647e-49	1.865953e-46
MsG0280006590.01	MsG0280008894.01	0.817974	2.496161e-52	1.844663e-49
MsG0280006590.01	MsG0280010349.01	0.808447	3.083950e-50	1.763793e-47
MsG0280006590.01	MsG0280010517.01	0.801214	1.000774e-48	4.752436e-46
MsG0280006590.01	MsG0280010598.01	0.801325	9.498948e-49	4.523365e-46
MsG0280006590.01	MsG0380011895.01	0.822810	1.940867e-53	1.641813e-50
MsG0280006590.01	MsG0380012005.01	0.817738	2.822146e-52	2.072275e-49
MsG0280006590.01	MsG0380013853.01	0.811331	7.385134e-51	4.558309e-48
MsG0280006590.01	MsG0380014251.01	0.807551	4.782554e-50	2.671597e-47
MsG0280006590.01	MsG0380016182.01	0.813853	2.074333e-51	1.369909e-48
MsG0280006590.01	MsG0380017220.01	0.800577	1.350202e-48	6.309141e-46
MsG0280006590.01	MsG0380017326.01	0.824155	9.409769e-54	8.267746e-51
MsG0280006590.01	MsG0380017543.01	0.818205	2.213331e-52	1.646143e-49
MsG0280006590.01	MsG0480018121.01	0.803224	3.860304e-49	1.929244e-46
MsG0280006590.01	MsG0480018236.01	0.825542	4.429975e-54	4.049649e-51
MsG0280006590.01	MsG0480020322.01	0.809395	1.932679e-50	1.133474e-47
MsG0280006590.01	MsG0480020323.01	0.820854	5.504174e-53	4.406011e-50
MsG0280006590.01	MsG0480022068.01	0.808921	2.442398e-50	1.414261e-47
MsG0280006590.01	MsG0480022145.01	0.804088	2.555404e-49	1.305778e-46
MsG0280006590.01	MsG0480022178.01	0.803169	3.963696e-49	1.978157e-46
MsG0280006590.01	MsG0480022802.01	0.800830	1.198621e-48	5.636950e-46
MsG0280006590.01	MsG0480023045.01	0.801825	7.498699e-49	3.616403e-46
MsG0280006590.01	MsG0480024015.01	0.805932	1.051272e-49	5.632934e-47
MsG0280006590.01	MsG0580024130.01	0.801470	8.870012e-49	4.239363e-46
MsG0280006590.01	MsG0580025467.01	0.825226	5.261368e-54	4.766974e-51
MsG0280006590.01	MsG0580026032.01	0.810116	1.352664e-50	8.085298e-48
MsG0280006590.01	MsG0580026304.01	0.806992	6.282470e-50	3.458851e-47
MsG0280006590.01	MsG0580026340.01	0.805745	1.150844e-49	6.137217e-47
MsG0280006590.01	MsG0680030992.01	0.800386	1.477001e-48	6.868286e-46
MsG0280006590.01	MsG0680031334.01	0.808040	3.764893e-50	2.130193e-47
MsG0280006590.01	MsG0680031355.01	0.807024	6.184549e-50	3.407854e-47
MsG0280006590.01	MsG0680031377.01	-0.805346	1.395651e-49	7.366229e-47
MsG0280006590.01	MsG0680032157.01	0.809410	1.918535e-50	1.125641e-47
MsG0280006590.01	MsG0780036128.01	0.862910	3.625635e-64	1.079536e-60
MsG0280006590.01	MsG0780036978.01	0.806279	8.884858e-50	4.803004e-47
MsG0280006590.01	MsG0780037105.01	0.806727	7.148933e-50	3.909026e-47
MsG0280006590.01	MsG0780038827.01	0.811260	7.653750e-51	4.715450e-48
MsG0280006590.01	MsG0780038833.01	0.800958	1.128689e-48	5.325594e-46
MsG0280006590.01	MsG0780039143.01	0.809743	1.626930e-50	9.630506e-48
MsG0280006590.01	MsG0780039498.01	0.825485	4.568545e-54	4.169617e-51
MsG0280006590.01	MsG0780040576.01	-0.802914	4.474932e-49	2.218805e-46
MsG0280006590.01	MsG0780041080.01	0.811916	5.510165e-51	3.454116e-48
MsG0280006590.01	MsG0880042247.01	0.827550	1.470548e-54	1.424235e-51
MsG0280006590.01	MsG0880042616.01	0.811357	7.290026e-51	4.502667e-48
MsG0280006590.01	MsG0880042622.01	0.809118	2.215583e-50	1.289817e-47
MsG0280006590.01	MsG0880043509.01	0.812987	3.214307e-51	2.073520e-48
MsG0280006590.01	MsG0880044573.01	0.812667	3.778159e-51	2.416733e-48
MsG0280006590.01	MsG0880045926.01	0.808542	2.942272e-50	1.686899e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006590.01.T01	MTR_2g011280	92.453	53	4	0	1	53	789	841	2.74e-27	102
MsG0280006590.01.T01	MTR_2g011270	92.453	53	4	0	1	53	769	821	9.25e-27	100
MsG0280006590.01.T01	MTR_2g011340	92.308	52	4	0	1	52	785	836	6.60e-26	97.8
MsG0280006590.01.T01	MTR_2g011230	81.132	53	10	0	1	53	780	832	3.80e-23	90.1
MsG0280006590.01.T01	MTR_2g011240	77.358	53	7	1	1	53	767	814	6.31e-21	84.0
MsG0280006590.01.T01	MTR_7g056663	54.545	55	23	1	1	53	790	844	3.73e-12	58.9
MsG0280006590.01.T01	MTR_7g056510	52.830	53	23	1	1	53	772	822	4.59e-12	58.5
MsG0280006590.01.T01	MTR_7g056650	53.846	52	22	1	1	50	620	671	6.03e-12	58.2
MsG0280006590.01.T01	MTR_7g056680	50.909	55	25	1	1	53	605	659	2.02e-11	56.6
MsG0280006590.01.T01	MTR_7g056680	50.909	55	25	1	1	53	770	824	3.09e-11	56.2
MsG0280006590.01.T01	MTR_7g056680	50.909	55	25	1	1	53	761	815	3.12e-11	56.2
MsG0280006590.01.T01	MTR_7g056680	50.909	55	25	1	1	53	765	819	3.15e-11	56.2
MsG0280006590.01.T01	MTR_7g056680	50.909	55	25	1	1	53	766	820	3.15e-11	56.2
MsG0280006590.01.T01	MTR_7g056430	48.214	56	26	1	1	53	1104	1159	3.48e-11	56.2
MsG0280006590.01.T01	MTR_7g056640	49.091	55	26	1	1	53	781	835	5.56e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTCAGTCTGTCTTAGA+AGG	0.391393	2:+3783767	None:intergenic
AGGAATGCAGGTTGAGATGG+AGG	0.451113	2:+3783787	None:intergenic
CATTGAAACAACTGTGGCCA+TGG	0.514839	2:+3783837	None:intergenic
GTCACTGTCACAAGCTTGCA+AGG	0.537803	2:-3783703	MsG0280006590.01.T01:CDS
GTCTGTCTTAGAAGGAATGC+AGG	0.539479	2:+3783775	None:intergenic
AATGCAGGTTGAGATGGAGG+AGG	0.550849	2:+3783790	None:intergenic
AGAAGGAATGCAGGTTGAGA+TGG	0.553513	2:+3783784	None:intergenic
ATTAAGCATTGAAACAACTG+TGG	0.698314	2:+3783831	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAGCATTGAAACAACTG+TGG	+	Chr2:3783703-3783722	None:intergenic	30.0%
	TATGCTCAGTCTGTCTTAGA+AGG	+	Chr2:3783767-3783786	None:intergenic	40.0%
	GTCTGTCTTAGAAGGAATGC+AGG	+	Chr2:3783759-3783778	None:intergenic	45.0%
!	AGAAGGAATGCAGGTTGAGA+TGG	+	Chr2:3783750-3783769	None:intergenic	45.0%
	GTCACTGTCACAAGCTTGCA+AGG	-	Chr2:3783828-3783847	MsG0280006590.01.T01:CDS	50.0%
!	AATGCAGGTTGAGATGGAGG+AGG	+	Chr2:3783744-3783763	None:intergenic	50.0%
!	AGGAATGCAGGTTGAGATGG+AGG	+	Chr2:3783747-3783766	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	3783696	3783857	3783696	ID=MsG0280006590.01;Name=MsG0280006590.01
Chr2	mRNA	3783696	3783857	3783696	ID=MsG0280006590.01.T01;Parent=MsG0280006590.01;Name=MsG0280006590.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|53
Chr2	exon	3783696	3783857	3783696	ID=MsG0280006590.01.T01:exon:2223;Parent=MsG0280006590.01.T01
Chr2	CDS	3783696	3783857	3783696	ID=MsG0280006590.01.T01:cds;Parent=MsG0280006590.01.T01

Gene Sequence	>MsG0280006590.01.T01 ATGGCCACAGTTGTTTCAATGCTTAATAGTGAGATTGTTAAACTTCCTCCTCCATCTCAACCTGCATTCCTTCTAAGACAGACTGAGCATAGAGCAGACTCAGCTCAACAAAATCACGACTCGAATAACAGTGTCACTGTCACAAGCTTGCAAGGCAGATAG
Protein sequence	>MsG0280006590.01.T01 MATVVSMLNSEIVKLPPPSQPAFLLRQTEHRADSAQQNHDSNNSVTVTSLQGR*