AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030992.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030992.01.T02 MTR_6g015780 93.220 59 4 0 1 59 48 106 2.53e-35 114
MsG0680030992.01.T02 MTR_6g015770 91.525 59 5 0 1 59 84 142 4.66e-35 115
MsG0680030992.01.T02 MTR_6g015715 92.857 28 2 0 1 28 48 75 2.54e-12 55.8
MsG0680030992.01.T01 MTR_6g015780 94.286 105 6 0 1 105 2 106 3.01e-59 177
MsG0680030992.01.T01 MTR_6g015770 93.103 87 6 0 19 105 56 142 5.75e-56 170
MsG0680030992.01.T01 MTR_6g015715 93.243 74 5 0 1 74 2 75 9.92e-45 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGCTTGCCTTCTCAAAA+TGG 0.248342 6:-12623416 MsG0680030992.01.T01:CDS
TCATGCAGCAAGAATGCTTT+TGG 0.265450 6:-12623519 MsG0680030992.01.T01:CDS
ACAACATCATTGAAGATAAA+AGG 0.287321 6:-12623605 MsG0680030992.01.T01:CDS
TCAAGATATTGGAATAATCT+TGG 0.343890 6:-12623312 MsG0680030992.01.T01:three_prime_UTR
TTGTCTGATCGACAATGATT+TGG 0.382602 6:-12623567 MsG0680030992.01.T01:CDS
CACATATATATAAACACAAA+TGG 0.420114 6:+12623281 None:intergenic
GTTAAGTGTCCTCCAGTTCA+AGG 0.438505 6:-12623446 MsG0680030992.01.T01:CDS
CAAACACAAACTGGTAGAAC+TGG 0.449515 6:-12623488 MsG0680030992.01.T01:CDS
CAAGTACGATAGCCTTGAAC+TGG 0.453081 6:+12623434 None:intergenic
GATTTGAGTCAAACACAAAC+TGG 0.464256 6:-12623497 MsG0680030992.01.T01:CDS
ATGGAGGTGGACCCAACAAT+AGG 0.474022 6:-12623397 MsG0680030992.01.T01:CDS
AATATCTTGAATTAATGAAA+AGG 0.484100 6:+12623325 None:intergenic
TAAGTACCACACCTATTGTT+GGG 0.495822 6:+12623386 None:intergenic
GTAAGTACCACACCTATTGT+TGG 0.525645 6:+12623385 None:intergenic
GTAATTGGATCAATAAGATA+AGG 0.548478 6:+12623639 None:intergenic
TTGCCTTCTCAAAATGGAGG+TGG 0.550109 6:-12623410 MsG0680030992.01.T01:CDS
GTGAATAATTCCATGTGAGA+TGG 0.555892 6:-12623185 MsG0680030992.01.T01:three_prime_UTR
GTACGATAGCCTTGAACTGG+AGG 0.617148 6:+12623437 None:intergenic
TGCTTGCCTTCTCAAAATGG+AGG 0.620754 6:-12623413 MsG0680030992.01.T01:CDS
ACCAAAGAAGAAAATGAACA+AGG 0.627471 6:-12623678 None:intergenic
CCAAAGAAGAAAATGAACAA+GGG 0.643796 6:-12623677 None:intergenic
GATAAAAGGTTGTACCTCAG+AGG 0.662136 6:-12623591 MsG0680030992.01.T01:CDS
GGTAGAACTGGTAACAACAA+TGG 0.669601 6:-12623476 MsG0680030992.01.T01:CDS
GGTGGACCCAACAATAGGTG+TGG 0.689105 6:-12623392 MsG0680030992.01.T01:CDS
GTCGATCAGACAATCCTCTG+AGG 0.727668 6:+12623577 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCTTGAATTAATGAAA+AGG + Chr6:12623519-12623538 None:intergenic 15.0%
!!! TTTTCATTAATTCAAGATAT+TGG - Chr6:12623518-12623537 MsG0680030992.01.T01:CDS 15.0%
!! CACATATATATAAACACAAA+TGG + Chr6:12623563-12623582 None:intergenic 20.0%
! ACAACATCATTGAAGATAAA+AGG - Chr6:12623236-12623255 MsG0680030992.01.T01:three_prime_UTR 25.0%
! GTAATTGGATCAATAAGATA+AGG + Chr6:12623205-12623224 None:intergenic 25.0%
! TCAAGATATTGGAATAATCT+TGG - Chr6:12623529-12623548 MsG0680030992.01.T01:CDS 25.0%
!! TTGTTTTGCTTAGGTGTAAT+TGG + Chr6:12623220-12623239 None:intergenic 30.0%
!!! TCAATGATGTTGTTTTGCTT+AGG + Chr6:12623229-12623248 None:intergenic 30.0%
GATTTGAGTCAAACACAAAC+TGG - Chr6:12623344-12623363 MsG0680030992.01.T01:three_prime_UTR 35.0%
GTGAATAATTCCATGTGAGA+TGG - Chr6:12623656-12623675 MsG0680030992.01.T01:CDS 35.0%
TAAGTACCACACCTATTGTT+GGG + Chr6:12623458-12623477 None:intergenic 35.0%
TTGTCTGATCGACAATGATT+TGG - Chr6:12623274-12623293 MsG0680030992.01.T01:three_prime_UTR 35.0%
ACTTGCTTGCCTTCTCAAAA+TGG - Chr6:12623425-12623444 MsG0680030992.01.T01:CDS 40.0%
CAAACACAAACTGGTAGAAC+TGG - Chr6:12623353-12623372 MsG0680030992.01.T01:exon 40.0%
GATAAAAGGTTGTACCTCAG+AGG - Chr6:12623250-12623269 MsG0680030992.01.T01:three_prime_UTR 40.0%
GGTAGAACTGGTAACAACAA+TGG - Chr6:12623365-12623384 MsG0680030992.01.T01:exon 40.0%
GTAAGTACCACACCTATTGT+TGG + Chr6:12623459-12623478 None:intergenic 40.0%
! TCATGCAGCAAGAATGCTTT+TGG - Chr6:12623322-12623341 MsG0680030992.01.T01:three_prime_UTR 40.0%
GTTAAGTGTCCTCCAGTTCA+AGG - Chr6:12623395-12623414 MsG0680030992.01.T01:CDS 45.0%
TGCTTGCCTTCTCAAAATGG+AGG - Chr6:12623428-12623447 MsG0680030992.01.T01:CDS 45.0%
TTGCCTTCTCAAAATGGAGG+TGG - Chr6:12623431-12623450 MsG0680030992.01.T01:CDS 45.0%
! CAAGTACGATAGCCTTGAAC+TGG + Chr6:12623410-12623429 None:intergenic 45.0%
ATGGAGGTGGACCCAACAAT+AGG - Chr6:12623444-12623463 MsG0680030992.01.T01:CDS 50.0%
GTCGATCAGACAATCCTCTG+AGG + Chr6:12623267-12623286 None:intergenic 50.0%
! GTACGATAGCCTTGAACTGG+AGG + Chr6:12623407-12623426 None:intergenic 50.0%
!! GGTCCACCTCCATTTTGAGA+AGG + Chr6:12623437-12623456 None:intergenic 50.0%
GGTGGACCCAACAATAGGTG+TGG - Chr6:12623449-12623468 MsG0680030992.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 12623176 12623687 12623176 ID=MsG0680030992.01;Name=MsG0680030992.01
Chr6 mRNA 12623176 12623687 12623176 ID=MsG0680030992.01.T01;Parent=MsG0680030992.01;Name=MsG0680030992.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|194|105
Chr6 exon 12623176 12623687 12623176 ID=MsG0680030992.01.T01:exon:8963;Parent=MsG0680030992.01.T01
Chr6 CDS 12623370 12623687 12623370 ID=MsG0680030992.01.T01:cds;Parent=MsG0680030992.01.T01
Chr6 three_prime_UTR 12623176 12623369 12623176 ID=MsG0680030992.01.T01:three_prime_utr;Parent=MsG0680030992.01.T01
Chr6 mRNA 12623370 12623549 12623370 ID=MsG0680030992.01.T02;Parent=MsG0680030992.01;Name=MsG0680030992.01.T02;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|59
Chr6 exon 12623370 12623549 12623370 ID=MsG0680030992.01.T02:exon:8964;Parent=MsG0680030992.01.T02
Chr6 CDS 12623370 12623549 12623370 ID=MsG0680030992.01.T02:cds;Parent=MsG0680030992.01.T02
Gene Sequence

>MsG0680030992.01.T02

ATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCTCCAGTTCAAGGCTATCGTACTTGCTTGCCTTCTCAAAATGGAGGTGGACCCAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA

>MsG0680030992.01.T01

ATGAACAAGGGTGTATCAGTATTGTTCCTTATCTTATTGATCCAATTACACCTAAGCAAAACAACATCATTGAAGATAAAAGGTTGTACCTCAGAGGATTGTCTGATCGACAATGATTTGGAATCAGAAGAATTTTCTATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCTCCAGTTCAAGGCTATCGTACTTGCTTGCCTTCTCAAAATGGAGGTGGACCCAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA

Protein sequence

>MsG0680030992.01.T02

MSSHAARMLLDLSQTQTGRTGNNNGAAVKCPPVQGYRTCLPSQNGGGPNNRCGTYNRVC*

>MsG0680030992.01.T01

MNKGVSVLFLILLIQLHLSKTTSLKIKGCTSEDCLIDNDLESEEFSMSSHAARMLLDLSQTQTGRTGNNNGAAVKCPPVQGYRTCLPSQNGGGPNNRCGTYNRVC*