AlfalfaGEDB | MsG0280008886.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008886.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008886.01.T01	XP_013464057.1	95.745	141	6	0	1	141	1	141	6.95E-95	280

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008886.01.T01	A0A072V8C8	95.745	141	6	0	1	141	1	141	3.32e-95	280

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047999.01	MsG0280008886.01	0.806620	7.530632e-50	4.106275e-47
MsG0080048919.01	MsG0280008886.01	-0.805010	1.640478e-49	8.584101e-47
MsG0080049023.01	MsG0280008886.01	-0.845161	4.826754e-59	7.972148e-56
MsG0180000719.01	MsG0280008886.01	-0.812555	3.997488e-51	2.549551e-48
MsG0180002099.01	MsG0280008886.01	-0.800958	1.128708e-48	5.325671e-46
MsG0180003351.01	MsG0280008886.01	-0.809688	1.672477e-50	9.885245e-48
MsG0180004731.01	MsG0280008886.01	-0.831486	1.624697e-55	1.765611e-52
MsG0180004737.01	MsG0280008886.01	-0.818952	1.498783e-52	1.138037e-49
MsG0180004773.01	MsG0280008886.01	-0.800513	1.391224e-48	6.490262e-46
MsG0180005272.01	MsG0280008886.01	0.850770	1.369449e-60	2.711835e-57
MsG0280006381.01	MsG0280008886.01	-0.808392	3.168560e-50	1.809566e-47
MsG0280007288.01	MsG0280008886.01	-0.809279	2.046651e-50	1.196585e-47
MsG0280007291.01	MsG0280008886.01	-0.815609	8.469191e-52	5.866967e-49
MsG0280008886.01	MsG0280010227.01	-0.805223	1.481101e-49	7.792409e-47
MsG0280008886.01	MsG0280010240.01	0.843300	1.525596e-58	2.376701e-55
MsG0280008886.01	MsG0280010455.01	-0.801061	1.075234e-48	5.086650e-46
MsG0280008886.01	MsG0280010583.01	-0.832905	7.240728e-56	8.206429e-53
MsG0280008886.01	MsG0380014639.01	-0.822386	2.435301e-53	2.035505e-50
MsG0280008886.01	MsG0380014642.01	-0.811840	5.723133e-51	3.580496e-48
MsG0280008886.01	MsG0380014735.01	-0.810163	1.321262e-50	7.906983e-48
MsG0280008886.01	MsG0380015417.01	-0.802536	5.355822e-49	2.630057e-46
MsG0280008886.01	MsG0380015654.01	-0.807591	4.690303e-50	2.622678e-47
MsG0280008886.01	MsG0380015828.01	-0.811653	6.287618e-51	3.913907e-48
MsG0280008886.01	MsG0380017812.01	-0.835658	1.476015e-56	1.816854e-53
MsG0280008886.01	MsG0480018201.01	-0.806803	6.887700e-50	3.773669e-47
MsG0280008886.01	MsG0480018202.01	-0.801554	8.526029e-49	4.083563e-46
MsG0280008886.01	MsG0480019304.01	0.811310	7.463426e-51	4.604100e-48
MsG0280008886.01	MsG0480023152.01	-0.801381	9.248274e-49	4.410322e-46
MsG0280008886.01	MsG0480023242.01	-0.810658	1.032932e-50	6.263212e-48
MsG0280008886.01	MsG0480023695.01	-0.816600	5.086767e-52	3.620356e-49
MsG0280008886.01	MsG0580024398.01	0.814746	1.316812e-51	8.909257e-49
MsG0280008886.01	MsG0580024442.01	-0.824873	6.375808e-54	5.718966e-51
MsG0280008886.01	MsG0580024448.01	-0.810001	1.431737e-50	8.532511e-48
MsG0280008886.01	MsG0580025105.01	-0.813512	2.464906e-51	1.612988e-48
MsG0280008886.01	MsG0580027276.01	-0.811112	8.237904e-51	5.055725e-48
MsG0280008886.01	MsG0580027797.01	0.813475	2.512051e-51	1.642171e-48
MsG0280008886.01	MsG0580030179.01	0.813040	3.130573e-51	2.022361e-48
MsG0280008886.01	MsG0680031686.01	0.806055	9.905891e-50	5.324472e-47
MsG0280008886.01	MsG0680035584.01	-0.817483	3.222297e-52	2.349604e-49
MsG0280008886.01	MsG0780037458.01	-0.813488	2.495026e-51	1.631684e-48
MsG0280008886.01	MsG0780039838.01	-0.817450	3.276557e-52	2.387069e-49
MsG0280008886.01	MsG0780039839.01	-0.805460	1.320839e-49	6.992021e-47
MsG0280008886.01	MsG0780041691.01	-0.800281	1.551351e-48	7.194958e-46
MsG0280008886.01	MsG0880043550.01	0.800644	1.308649e-48	6.125310e-46
MsG0280008886.01	MsG0880046010.01	-0.820374	7.095714e-53	5.604820e-50
MsG0280008886.01	MsG0880046630.01	-0.800773	1.231268e-48	5.781909e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008886.01.T01	MTR_2g461190	95.745	141	6	0	1	141	1	141	8.41e-99	280
MsG0280008886.01.T01	MTR_4g116530	57.718	149	53	5	1	140	1	148	2.78e-48	152
MsG0280008886.01.T01	MTR_3g078240	48.000	75	33	3	66	134	51	125	9.81e-15	67.0
MsG0280008886.01.T01	MTR_6g007727	46.154	78	33	3	59	130	63	137	4.10e-14	65.5
MsG0280008886.01.T01	MTR_4g106650	46.835	79	35	4	57	130	55	131	6.09e-13	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008886.01.T01	AT5G25240	39.024	123	59	5	18	131	11	126	3.27e-16	70.5
MsG0280008886.01.T01	AT3G01430	32.374	139	56	5	28	131	26	161	1.67e-15	69.7
MsG0280008886.01.T01	AT5G14895	40.449	89	40	3	56	131	60	148	3.00e-13	63.5

Find 41 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGTGTTTGTGGGTTGAAA+TGG	0.310334	2:-40937369	MsG0280008886.01.T01:CDS
CATAGCTGTGTTCATCATAT+TGG	0.336315	2:+40937176	None:intergenic
TCGTGTTTGTGGGTTGAAAT+GGG	0.336472	2:-40937368	MsG0280008886.01.T01:CDS
CACCAAGAGGCTAGTGGATG+TGG	0.398723	2:-40937403	MsG0280008886.01.T01:CDS
ATTAGAAAGATTAGCATGTA+TGG	0.403666	2:-40937223	MsG0280008886.01.T01:CDS
TGGAGCAAAGGGAAGATTCT+TGG	0.403735	2:-40937315	MsG0280008886.01.T01:CDS
TATGAGTGCTTTCGTGTTTG+TGG	0.423020	2:-40937379	MsG0280008886.01.T01:CDS
AAGCCCATTGGTTGAGGCGG+TGG	0.425680	2:-40937452	MsG0280008886.01.T01:CDS
TGTTCCACCGCCTCAACCAA+TGG	0.453542	2:+40937448	None:intergenic
TGATGAAGGTAAAGGTCTAC+TGG	0.454979	2:-40937335	MsG0280008886.01.T01:CDS
GAAGTTTCAGAAGTGATTGC+AGG	0.459250	2:-40937265	MsG0280008886.01.T01:CDS
ATGAGTGCTTTCGTGTTTGT+GGG	0.459623	2:-40937378	MsG0280008886.01.T01:CDS
CTAATGAATGTCTTCCACTT+AGG	0.465024	2:+40937241	None:intergenic
TGAGGGAAGCCCATTGGTTG+AGG	0.474777	2:-40937458	MsG0280008886.01.T01:CDS
GTATGGTAAGAAGCAACATA+AGG	0.478077	2:-40937206	MsG0280008886.01.T01:CDS
CGTGTTTGTGGGTTGAAATG+GGG	0.493007	2:-40937367	MsG0280008886.01.T01:CDS
TTGCAAGTTGAGGAAGATGA+AGG	0.493022	2:-40937287	MsG0280008886.01.T01:CDS
TTTCCCTCTGGACGTCGCCA+GGG	0.504330	2:-40937076	MsG0280008886.01.T01:CDS
ACCCACATCCACTAGCCTCT+TGG	0.506045	2:+40937401	None:intergenic
GTTCCACCGCCTCAACCAAT+GGG	0.506673	2:+40937449	None:intergenic
ATGCTCTCAACTTCAGTAGT+GGG	0.519153	2:-40937156	MsG0280008886.01.T01:CDS
CTTGGTGGGTTTGCAAGTTG+AGG	0.524199	2:-40937297	MsG0280008886.01.T01:CDS
TGGCGACGTCCAGAGGGAAA+AGG	0.529482	2:+40937079	None:intergenic
GTGTTTGTGGGTTGAAATGG+GGG	0.532525	2:-40937366	MsG0280008886.01.T01:CDS
TTCACCCTGGCGACGTCCAG+AGG	0.534141	2:+40937072	None:intergenic
TAAAGGTCTACTGGAGCAAA+GGG	0.537104	2:-40937326	MsG0280008886.01.T01:CDS
TATGGTAAGAAGCAACATAA+GGG	0.539440	2:-40937205	MsG0280008886.01.T01:CDS
GTAAAGGTCTACTGGAGCAA+AGG	0.569320	2:-40937327	MsG0280008886.01.T01:CDS
AAGTGATTGCAGGTCCTAAG+TGG	0.573683	2:-40937255	MsG0280008886.01.T01:CDS
TATGCTCTCAACTTCAGTAG+TGG	0.578055	2:-40937157	MsG0280008886.01.T01:CDS
GCAAAGGGAAGATTCTTGGT+GGG	0.584946	2:-40937311	MsG0280008886.01.T01:CDS
CAAGGCAATGATGAAGGTAA+AGG	0.587419	2:-40937343	MsG0280008886.01.T01:CDS
AATGATCACCAAGAGGCTAG+TGG	0.598393	2:-40937409	MsG0280008886.01.T01:CDS
GGGAAGCCCATTGGTTGAGG+CGG	0.598814	2:-40937455	MsG0280008886.01.T01:CDS
TTGGTTGAGGCGGTGGAACA+CGG	0.607703	2:-40937445	MsG0280008886.01.T01:CDS
AGCAAAGGGAAGATTCTTGG+TGG	0.616895	2:-40937312	MsG0280008886.01.T01:CDS
TCACCCTGGCGACGTCCAGA+GGG	0.620675	2:+40937073	None:intergenic
ACCAAGAGGCTAGTGGATGT+GGG	0.624825	2:-40937402	MsG0280008886.01.T01:CDS
TGCTCTCAACTTCAGTAGTG+GGG	0.635696	2:-40937155	MsG0280008886.01.T01:CDS
GCTTGAATCACAGTTCACCC+TGG	0.661600	2:+40937059	None:intergenic
AGATTTCAATGATCACCAAG+AGG	0.680638	2:-40937416	MsG0280008886.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTAGAAAGATTAGCATGTA+TGG	-	Chr2:40937312-40937331	MsG0280008886.01.T01:CDS	25.0%
	TATGGTAAGAAGCAACATAA+GGG	-	Chr2:40937330-40937349	MsG0280008886.01.T01:CDS	30.0%
	AGATTTCAATGATCACCAAG+AGG	-	Chr2:40937119-40937138	MsG0280008886.01.T01:CDS	35.0%
	CATAGCTGTGTTCATCATAT+TGG	+	Chr2:40937362-40937381	None:intergenic	35.0%
	CTAATGAATGTCTTCCACTT+AGG	+	Chr2:40937297-40937316	None:intergenic	35.0%
	GTATGGTAAGAAGCAACATA+AGG	-	Chr2:40937329-40937348	MsG0280008886.01.T01:CDS	35.0%
	AATCTAGCCGAAAAACTGTG+CGG	+	Chr2:40937429-40937448	None:intergenic	40.0%
	ATGAGTGCTTTCGTGTTTGT+GGG	-	Chr2:40937157-40937176	MsG0280008886.01.T01:CDS	40.0%
	ATGCTCTCAACTTCAGTAGT+GGG	-	Chr2:40937379-40937398	MsG0280008886.01.T01:CDS	40.0%
	CAAGGCAATGATGAAGGTAA+AGG	-	Chr2:40937192-40937211	MsG0280008886.01.T01:CDS	40.0%
	GAAGTTTCAGAAGTGATTGC+AGG	-	Chr2:40937270-40937289	MsG0280008886.01.T01:CDS	40.0%
	TAAAGGTCTACTGGAGCAAA+GGG	-	Chr2:40937209-40937228	MsG0280008886.01.T01:CDS	40.0%
	TATGAGTGCTTTCGTGTTTG+TGG	-	Chr2:40937156-40937175	MsG0280008886.01.T01:CDS	40.0%
	TATGCTCTCAACTTCAGTAG+TGG	-	Chr2:40937378-40937397	MsG0280008886.01.T01:CDS	40.0%
	TGATGAAGGTAAAGGTCTAC+TGG	-	Chr2:40937200-40937219	MsG0280008886.01.T01:CDS	40.0%
!	TCGTGTTTGTGGGTTGAAAT+GGG	-	Chr2:40937167-40937186	MsG0280008886.01.T01:CDS	40.0%
!	TTCGTGTTTGTGGGTTGAAA+TGG	-	Chr2:40937166-40937185	MsG0280008886.01.T01:CDS	40.0%
!	TTGCAAGTTGAGGAAGATGA+AGG	-	Chr2:40937248-40937267	MsG0280008886.01.T01:CDS	40.0%
	AAGTGATTGCAGGTCCTAAG+TGG	-	Chr2:40937280-40937299	MsG0280008886.01.T01:CDS	45.0%
	AATGATCACCAAGAGGCTAG+TGG	-	Chr2:40937126-40937145	MsG0280008886.01.T01:CDS	45.0%
	AGCAAAGGGAAGATTCTTGG+TGG	-	Chr2:40937223-40937242	MsG0280008886.01.T01:CDS	45.0%
	GCAAAGGGAAGATTCTTGGT+GGG	-	Chr2:40937224-40937243	MsG0280008886.01.T01:CDS	45.0%
	GTAAAGGTCTACTGGAGCAA+AGG	-	Chr2:40937208-40937227	MsG0280008886.01.T01:CDS	45.0%
	TGCTCTCAACTTCAGTAGTG+GGG	-	Chr2:40937380-40937399	MsG0280008886.01.T01:CDS	45.0%
	TGGAGCAAAGGGAAGATTCT+TGG	-	Chr2:40937220-40937239	MsG0280008886.01.T01:CDS	45.0%
!	CGTGTTTGTGGGTTGAAATG+GGG	-	Chr2:40937168-40937187	MsG0280008886.01.T01:CDS	45.0%
!	GTGTTTGTGGGTTGAAATGG+GGG	-	Chr2:40937169-40937188	MsG0280008886.01.T01:CDS	45.0%
!	TTTAGTGCTCCTTTTCCCTC+TGG	-	Chr2:40937447-40937466	MsG0280008886.01.T01:CDS	45.0%
!	ACCAAGAGGCTAGTGGATGT+GGG	-	Chr2:40937133-40937152	MsG0280008886.01.T01:CDS	50.0%
!	CGACTTGCCGCACAGTTTTT+CGG	-	Chr2:40937419-40937438	MsG0280008886.01.T01:CDS	50.0%
!!	CTTGGTGGGTTTGCAAGTTG+AGG	-	Chr2:40937238-40937257	MsG0280008886.01.T01:CDS	50.0%
!!	TGCTTTTGAGGGAAGCCCAT+TGG	-	Chr2:40937071-40937090	MsG0280008886.01.T01:CDS	50.0%
	GTTCCACCGCCTCAACCAAT+GGG	+	Chr2:40937089-40937108	None:intergenic	55.0%
	TGGGGGCAAGGCAATGATGA+AGG	-	Chr2:40937186-40937205	MsG0280008886.01.T01:CDS	55.0%
	TGTTCCACCGCCTCAACCAA+TGG	+	Chr2:40937090-40937109	None:intergenic	55.0%
	TTGGTTGAGGCGGTGGAACA+CGG	-	Chr2:40937090-40937109	MsG0280008886.01.T01:CDS	55.0%
!	ACCCACATCCACTAGCCTCT+TGG	+	Chr2:40937137-40937156	None:intergenic	55.0%
!	CACCAAGAGGCTAGTGGATG+TGG	-	Chr2:40937132-40937151	MsG0280008886.01.T01:CDS	55.0%
!	TGAGGGAAGCCCATTGGTTG+AGG	-	Chr2:40937077-40937096	MsG0280008886.01.T01:CDS	55.0%
!	TGTGGGTTGAAATGGGGGCA+AGG	-	Chr2:40937174-40937193	MsG0280008886.01.T01:CDS	55.0%
	TGGCGACGTCCAGAGGGAAA+AGG	+	Chr2:40937459-40937478	None:intergenic	60.0%
	TTTCCCTCTGGACGTCGCCA+GGG	-	Chr2:40937459-40937478	MsG0280008886.01.T01:CDS	60.0%
!	AAGCCCATTGGTTGAGGCGG+TGG	-	Chr2:40937083-40937102	MsG0280008886.01.T01:CDS	60.0%
!	GGGAAGCCCATTGGTTGAGG+CGG	-	Chr2:40937080-40937099	MsG0280008886.01.T01:CDS	60.0%
!	TTTTCCCTCTGGACGTCGCC+AGG	-	Chr2:40937458-40937477	MsG0280008886.01.T01:CDS	60.0%
	TCACCCTGGCGACGTCCAGA+GGG	+	Chr2:40937465-40937484	None:intergenic	65.0%
	TTCACCCTGGCGACGTCCAG+AGG	+	Chr2:40937466-40937485	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	40937066	40937491	40937066	ID=MsG0280008886.01;Name=MsG0280008886.01
Chr2	mRNA	40937066	40937491	40937066	ID=MsG0280008886.01.T01;Parent=MsG0280008886.01;Name=MsG0280008886.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|141
Chr2	exon	40937066	40937491	40937066	ID=MsG0280008886.01.T01:exon:30112;Parent=MsG0280008886.01.T01
Chr2	CDS	40937066	40937491	40937066	ID=MsG0280008886.01.T01:cds;Parent=MsG0280008886.01.T01

Gene Sequence

>MsG0280008886.01.T01

ATGGCTGCTTTTGAGGGAAGCCCATTGGTTGAGGCGGTGGAACACGGTAGAGAAGATTTCAATGATCACCAAGAGGCTAGTGGATGTGGGTATGAGTGCTTTCGTGTTTGTGGGTTGAAATGGGGGCAAGGCAATGATGAAGGTAAAGGTCTACTGGAGCAAAGGGAAGATTCTTGGTGGGTTTGCAAGTTGAGGAAGATGAAGGAAGTTTCAGAAGTGATTGCAGGTCCTAAGTGGAAGACATTCATTAGAAAGATTAGCATGTATGGTAAGAAGCAACATAAGGGTAAGTTCCAATATGATGAACACAGCTATGCTCTCAACTTCAGTAGTGGGGCACAGAGTGAAGATGACGACTTGCCGCACAGTTTTTCGGCTAGATTTAGTGCTCCTTTTCCCTCTGGACGTCGCCAGGGTGAACTGTGA

Protein sequence

>MsG0280008886.01.T01

MAAFEGSPLVEAVEHGREDFNDHQEASGCGYECFRVCGLKWGQGNDEGKGLLEQREDSWWVCKLRKMKEVSEVIAGPKWKTFIRKISMYGKKQHKGKFQYDEHSYALNFSSGAQSEDDDLPHSFSARFSAPFPSGRRQGEL*