AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010583.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010583.01.T01 MTR_2g087890 97.531 81 2 0 1 81 60 140 3.49e-51 166
MsG0280010583.01.T01 MTR_3g111530 53.846 78 34 2 1 78 129 204 1.77e-20 84.0
MsG0280010583.01.T01 MTR_1g021652 51.852 81 37 2 1 81 95 173 2.75e-20 83.6
MsG0280010583.01.T01 MTR_1g034320 51.852 81 37 2 1 81 52 130 2.60e-19 80.5
MsG0280010583.01.T01 MTR_1g034330 49.383 81 39 2 1 81 56 134 7.13e-18 76.6
MsG0280010583.01.T01 MTR_4g068550 46.835 79 40 1 1 79 49 125 2.83e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010583.01.T01 AT5G42650 50.000 76 36 1 3 78 91 164 1.40e-18 78.6
MsG0280010583.01.T01 AT4G15440 70.000 40 12 0 42 81 1 40 2.32e-14 66.6

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATTTACGGGAAATATT+AGG 0.183729 2:+71766231 MsG0280010583.01.T01:CDS
TTTCTCTCACCTCTTTGATA+TGG 0.280521 2:+71766160 MsG0280010583.01.T01:CDS
TGTTCCTAGTGTTGAATTTA+CGG 0.291492 2:+71766220 MsG0280010583.01.T01:CDS
TCAAGAACTGCAATAATATT+AGG 0.294757 2:-71766128 None:intergenic
TGAATTTACGGGAAATATTA+GGG 0.330709 2:+71766232 MsG0280010583.01.T01:CDS
GTTCCTAGTGTTGAATTTAC+GGG 0.375540 2:+71766221 MsG0280010583.01.T01:CDS
GGATTCACGTTGGTGAAGAA+AGG 0.453342 2:-71766107 None:intergenic
GACAAAAGAGATGTTCTAGT+TGG 0.460373 2:+71766191 MsG0280010583.01.T01:CDS
TTTACGGGAAATATTAGGGT+TGG 0.514210 2:+71766236 MsG0280010583.01.T01:CDS
GATTCACGTTGGTGAAGAAA+GGG 0.521739 2:-71766106 None:intergenic
TTCTGAACCTCAACATGCTA+AGG 0.543924 2:+71766274 MsG0280010583.01.T01:CDS
TTTCCCGTAAATTCAACACT+AGG 0.562457 2:-71766224 None:intergenic
AGTGGTACCTTAGCATGTTG+AGG 0.575238 2:-71766281 None:intergenic
AGGGAACGTGGGTGGAACAT+TGG 0.578719 2:-71766087 None:intergenic
GAAAGTAGGTGGTGTCATAG+TGG 0.592408 2:-71766299 None:intergenic
ACGTGGGTGGAACATTGGTA+CGG 0.634090 2:-71766082 None:intergenic
GTGAAGAAAGGGAACGTGGG+TGG 0.642657 2:-71766095 None:intergenic
CAACAAGGTCCATATCAAAG+AGG 0.642799 2:-71766169 None:intergenic
AATAATATTAGGATTCACGT+TGG 0.648803 2:-71766117 None:intergenic
GTTGGTGAAGAAAGGGAACG+TGG 0.649752 2:-71766099 None:intergenic
TTGGTGAAGAAAGGGAACGT+GGG 0.709718 2:-71766098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAATATTAGGATTCACGT+TGG - Chr2:71766120-71766139 None:intergenic 25.0%
! TCAAGAACTGCAATAATATT+AGG - Chr2:71766131-71766150 None:intergenic 25.0%
! TGAATTTACGGGAAATATTA+GGG + Chr2:71766232-71766251 MsG0280010583.01.T01:CDS 25.0%
! TTGAATTTACGGGAAATATT+AGG + Chr2:71766231-71766250 MsG0280010583.01.T01:CDS 25.0%
! TGTTCCTAGTGTTGAATTTA+CGG + Chr2:71766220-71766239 MsG0280010583.01.T01:CDS 30.0%
GACAAAAGAGATGTTCTAGT+TGG + Chr2:71766191-71766210 MsG0280010583.01.T01:CDS 35.0%
TTTACGGGAAATATTAGGGT+TGG + Chr2:71766236-71766255 MsG0280010583.01.T01:CDS 35.0%
TTTCCCGTAAATTCAACACT+AGG - Chr2:71766227-71766246 None:intergenic 35.0%
TTTCTCTCACCTCTTTGATA+TGG + Chr2:71766160-71766179 MsG0280010583.01.T01:CDS 35.0%
! GAACATCTCTTTTGTCAACA+AGG - Chr2:71766187-71766206 None:intergenic 35.0%
! GTTCCTAGTGTTGAATTTAC+GGG + Chr2:71766221-71766240 MsG0280010583.01.T01:CDS 35.0%
CAACAAGGTCCATATCAAAG+AGG - Chr2:71766172-71766191 None:intergenic 40.0%
TTCTGAACCTCAACATGCTA+AGG + Chr2:71766274-71766293 MsG0280010583.01.T01:CDS 40.0%
! GATTCACGTTGGTGAAGAAA+GGG - Chr2:71766109-71766128 None:intergenic 40.0%
AGTGGTACCTTAGCATGTTG+AGG - Chr2:71766284-71766303 None:intergenic 45.0%
TTGGTGAAGAAAGGGAACGT+GGG - Chr2:71766101-71766120 None:intergenic 45.0%
! GGATTCACGTTGGTGAAGAA+AGG - Chr2:71766110-71766129 None:intergenic 45.0%
ACGTGGGTGGAACATTGGTA+CGG - Chr2:71766085-71766104 None:intergenic 50.0%
! GTTGGTGAAGAAAGGGAACG+TGG - Chr2:71766102-71766121 None:intergenic 50.0%
AGGGAACGTGGGTGGAACAT+TGG - Chr2:71766090-71766109 None:intergenic 55.0%
GTGAAGAAAGGGAACGTGGG+TGG - Chr2:71766098-71766117 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 71766056 71766307 71766056 ID=MsG0280010583.01;Name=MsG0280010583.01
Chr2 mRNA 71766056 71766307 71766056 ID=MsG0280010583.01.T01;Parent=MsG0280010583.01;Name=MsG0280010583.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|83
Chr2 exon 71766056 71766307 71766056 ID=MsG0280010583.01.T01:exon:15174;Parent=MsG0280010583.01.T01
Chr2 CDS 71766056 71766307 71766056 ID=MsG0280010583.01.T01:cds;Parent=MsG0280010583.01.T01
Gene Sequence

>MsG0280010583.01.T01

ATGGACAAGTACAAGAGTACTGTATTCCGTACCAATGTTCCACCCACGTTCCCTTTCTTCACCAACGTGAATCCTAATATTATTGCAGTTCTTGATTGTAAGTCTTTCTCTCACCTCTTTGATATGGACCTTGTTGACAAAAGAGATGTTCTAGTTGGAGATTTTGTTCCTAGTGTTGAATTTACGGGAAATATTAGGGTTGGTGTTTATCAAGATGTTTCTGAACCTCAACATGCTAAGGTACCACTATGA

Protein sequence

>MsG0280010583.01.T01

MDKYKSTVFRTNVPPTFPFFTNVNPNIIAVLDCKSFSHLFDMDLVDKRDVLVGDFVPSVEFTGNIRVGVYQDVSEPQHAKVPL*