AlfalfaGEDB | MsG0380017038.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017038.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017038.01.T01	XP_013461686.1	97.436	78	2	0	1	78	1	78	1.47E-44	159

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017038.01.T01	Q8W490	55.128	78	30	2	1	78	1	73	7.28E-16	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017038.01.T01	A0A072V200	97.436	78	2	0	1	78	1	78	7.02e-45	159

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000101.01	MsG0380017038.01	0.833700	4.588238e-56	5.325002e-53
MsG0180000146.01	MsG0380017038.01	0.813169	2.933108e-51	1.901333e-48
MsG0180000555.01	MsG0380017038.01	0.800890	1.165446e-48	5.489569e-46
MsG0180000741.01	MsG0380017038.01	0.808346	3.240953e-50	1.848674e-47
MsG0180001911.01	MsG0380017038.01	0.809710	1.653861e-50	9.781092e-48
MsG0180002071.01	MsG0380017038.01	0.810528	1.102450e-50	6.661064e-48
MsG0180002709.01	MsG0380017038.01	0.806423	8.286037e-50	4.495657e-47
MsG0180003303.01	MsG0380017038.01	0.822712	2.046095e-53	1.725978e-50
MsG0180003744.01	MsG0380017038.01	0.821098	4.837623e-53	3.898766e-50
MsG0180004056.01	MsG0380017038.01	0.846381	2.251784e-59	3.868698e-56
MsG0180004252.01	MsG0380017038.01	0.817990	2.475726e-52	1.830437e-49
MsG0180004372.01	MsG0380017038.01	0.809198	2.130055e-50	1.242689e-47
MsG0180004598.01	MsG0380017038.01	0.820538	6.504779e-53	5.161201e-50
MsG0180004645.01	MsG0380017038.01	0.823737	1.178763e-53	1.023725e-50
MsG0180004730.01	MsG0380017038.01	0.814466	1.518905e-51	1.019987e-48
MsG0180005228.01	MsG0380017038.01	0.817359	3.434600e-52	2.495853e-49
MsG0180005985.01	MsG0380017038.01	0.814481	1.507061e-51	1.012444e-48
MsG0280010932.01	MsG0380017038.01	0.821284	4.381930e-53	3.549554e-50
MsG0280010976.01	MsG0380017038.01	0.830356	3.075765e-55	3.233017e-52
MsG0280011075.01	MsG0380017038.01	0.843173	1.648955e-58	2.558185e-55
MsG0380012458.01	MsG0380017038.01	0.805927	1.053649e-49	5.644928e-47
MsG0380014488.01	MsG0380017038.01	0.800904	1.157877e-48	5.455838e-46
MsG0380015179.01	MsG0380017038.01	0.804552	2.045053e-49	1.057519e-46
MsG0380015436.01	MsG0380017038.01	0.835242	1.880547e-56	2.286371e-53
MsG0380015607.01	MsG0380017038.01	0.806982	6.315043e-50	3.475904e-47
MsG0380016209.01	MsG0380017038.01	0.844447	7.516773e-59	1.213826e-55
MsG0380017038.01	MsG0380017039.01	0.820887	5.408224e-53	4.333159e-50
MsG0380017038.01	MsG0480018460.01	0.812863	3.422089e-51	2.200159e-48
MsG0380017038.01	MsG0480021204.01	0.841116	5.776312e-58	8.401808e-55
MsG0380017038.01	MsG0480021216.01	0.808752	2.654324e-50	1.530151e-47
MsG0380017038.01	MsG0480022026.01	0.821167	4.663055e-53	3.765169e-50
MsG0380017038.01	MsG0480022108.01	0.806012	1.011463e-49	5.430764e-47
MsG0380017038.01	MsG0480022318.01	0.818643	1.761301e-52	1.325808e-49
MsG0380017038.01	MsG0480022552.01	0.812090	5.049006e-51	3.180227e-48
MsG0380017038.01	MsG0480022678.01	0.801433	9.025167e-49	4.309549e-46
MsG0380017038.01	MsG0580024770.01	0.840040	1.104368e-57	1.554075e-54
MsG0380017038.01	MsG0580025128.01	0.824245	8.960892e-54	7.893199e-51
MsG0380017038.01	MsG0580025184.01	0.807339	5.305954e-50	2.947749e-47
MsG0380017038.01	MsG0580026664.01	0.800817	1.205974e-48	5.669522e-46
MsG0380017038.01	MsG0580028346.01	0.800842	1.192324e-48	5.609037e-46
MsG0380017038.01	MsG0680030684.01	0.800476	1.415860e-48	6.598842e-46
MsG0380017038.01	MsG0680034067.01	0.804441	2.157474e-49	1.112450e-46
MsG0380017038.01	MsG0780036836.01	0.816331	5.844925e-52	4.129641e-49
MsG0380017038.01	MsG0780039587.01	0.837777	4.254273e-57	5.584939e-54
MsG0380017038.01	MsG0780040687.01	0.802665	5.035924e-49	2.481085e-46
MsG0380017038.01	MsG0780040742.01	0.830132	3.489030e-55	3.643586e-52
MsG0380017038.01	MsG0780040845.01	0.816382	5.693337e-52	4.027994e-49
MsG0380017038.01	MsG0780041033.01	0.811824	5.771981e-51	3.609446e-48
MsG0380017038.01	MsG0780041222.01	0.821996	3.000379e-53	2.479747e-50
MsG0380017038.01	MsG0780041278.01	0.812720	3.678989e-51	2.356624e-48
MsG0380017038.01	MsG0780041370.01	0.824558	7.563719e-54	6.723200e-51
MsG0380017038.01	MsG0880042206.01	0.802244	6.151204e-49	2.998308e-46
MsG0380017038.01	MsG0880042783.01	0.814181	1.755623e-51	1.169836e-48
MsG0380017038.01	MsG0880043341.01	0.807682	4.486607e-50	2.514813e-47
MsG0380017038.01	MsG0880043533.01	0.818014	2.445089e-52	1.808950e-49
MsG0380017038.01	MsG0880044546.01	0.823496	1.342728e-53	1.158067e-50
MsG0380017038.01	MsG0880045165.01	0.839355	1.664881e-57	2.293479e-54
MsG0380017038.01	MsG0880045450.01	0.807835	4.161784e-50	2.342128e-47
MsG0380017038.01	MsG0880046047.01	0.816688	4.860475e-52	3.467584e-49
MsG0380017038.01	MsG0880046535.01	0.804517	2.079981e-49	1.074566e-46
MsG0380017038.01	MsG0880046643.01	0.800138	1.658552e-48	7.664240e-46
MsG0380017038.01	MsG0880047745.01	0.826982	2.011279e-54	1.916064e-51
MsG0280006462.01	MsG0380017038.01	0.813531	2.441410e-51	1.598462e-48
MsG0280007087.01	MsG0380017038.01	0.866555	2.618308e-65	8.861837e-62
MsG0280007726.01	MsG0380017038.01	0.801073	1.069168e-48	5.059413e-46
MsG0280007829.01	MsG0380017038.01	0.809035	2.308498e-50	1.340854e-47
MsG0280007830.01	MsG0380017038.01	0.818298	2.108865e-52	1.572442e-49
MsG0280010531.01	MsG0380017038.01	0.812851	3.443896e-51	2.213471e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017038.01.T01	MTR_3g099767	97.436	78	2	0	1	78	1	78	1.78e-48	159

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017038.01.T01	AT1G12680	55.128	78	30	2	1	78	1	73	7.42e-17	73.6

Find 16 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATCTGGGTGTTGTTAATT+TGG	0.196464	3:+91063295	MsG0380017038.01.T01:CDS
CATTGCTACTGCTCCGCCTT+TGG	0.251885	3:+91063463	MsG0380017038.01.T01:CDS
ATTGCTACTGCTCCGCCTTT+GGG	0.294492	3:+91063464	MsG0380017038.01.T01:CDS
TCCTTTAAAAGTAGGCTTGC+TGG	0.333477	3:+91063440	MsG0380017038.01.T01:CDS
ACTCTTTAGAAGATTGTTGT+AGG	0.336360	3:+91063366	MsG0380017038.01.T01:CDS
AGAGTAATGAGACTTCAAAT+TGG	0.397426	3:-91063348	None:intergenic
TTGAAGGTTCCTTTAAAAGT+AGG	0.407569	3:+91063432	MsG0380017038.01.T01:CDS
GACACTCACATTGAATTTGA+AGG	0.414868	3:+91063416	MsG0380017038.01.T01:CDS
AACTGATGCATCTACGGATC+TGG	0.426906	3:+91063280	MsG0380017038.01.T01:CDS
TTAGATCACGCTGGTTCTGT+TGG	0.447839	3:+91063320	MsG0380017038.01.T01:CDS
ATTATGAGGAAGAAAAGAAA+AGG	0.483921	3:+91063251	None:intergenic
AAGTGAAACTGATGCATCTA+CGG	0.527123	3:+91063274	MsG0380017038.01.T01:CDS
ACTGATGCATCTACGGATCT+GGG	0.577067	3:+91063281	MsG0380017038.01.T01:CDS
AATCAAAGACGACGTCCCAA+AGG	0.588050	3:-91063479	None:intergenic
CAAAGACGACGTCCCAAAGG+CGG	0.588247	3:-91063476	None:intergenic
AATTTGGATTTAGATCACGC+TGG	0.689781	3:+91063311	MsG0380017038.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGAGTAATGAGACTTCAAAT+TGG	-	Chr3:91063351-91063370	None:intergenic	30.0%
!	ACTCTTTAGAAGATTGTTGT+AGG	+	Chr3:91063366-91063385	MsG0380017038.01.T01:CDS	30.0%
!	TTGAAGGTTCCTTTAAAAGT+AGG	+	Chr3:91063432-91063451	MsG0380017038.01.T01:CDS	30.0%
	AAGTGAAACTGATGCATCTA+CGG	+	Chr3:91063274-91063293	MsG0380017038.01.T01:CDS	35.0%
	AATTTGGATTTAGATCACGC+TGG	+	Chr3:91063311-91063330	MsG0380017038.01.T01:CDS	35.0%
	GACACTCACATTGAATTTGA+AGG	+	Chr3:91063416-91063435	MsG0380017038.01.T01:CDS	35.0%
!	TCCTTTAAAAGTAGGCTTGC+TGG	+	Chr3:91063440-91063459	MsG0380017038.01.T01:CDS	40.0%
!!	GGATCTGGGTGTTGTTAATT+TGG	+	Chr3:91063295-91063314	MsG0380017038.01.T01:CDS	40.0%
	AACTGATGCATCTACGGATC+TGG	+	Chr3:91063280-91063299	MsG0380017038.01.T01:CDS	45.0%
	ACTGATGCATCTACGGATCT+GGG	+	Chr3:91063281-91063300	MsG0380017038.01.T01:CDS	45.0%
!!	GCCAGCAAGCCTACTTTTAA+AGG	-	Chr3:91063444-91063463	None:intergenic	45.0%
!!	TTAGATCACGCTGGTTCTGT+TGG	+	Chr3:91063320-91063339	MsG0380017038.01.T01:CDS	45.0%
	ATTGCTACTGCTCCGCCTTT+GGG	+	Chr3:91063464-91063483	MsG0380017038.01.T01:CDS	50.0%
	CAAAGACGACGTCCCAAAGG+CGG	-	Chr3:91063479-91063498	None:intergenic	55.0%
	CATTGCTACTGCTCCGCCTT+TGG	+	Chr3:91063463-91063482	MsG0380017038.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	91063254	91063499	91063254	ID=MsG0380017038.01;Name=MsG0380017038.01
Chr3	mRNA	91063254	91063499	91063254	ID=MsG0380017038.01.T01;Parent=MsG0380017038.01;Name=MsG0380017038.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
Chr3	exon	91063254	91063499	91063254	ID=MsG0380017038.01.T01:exon:5177;Parent=MsG0380017038.01.T01
Chr3	CDS	91063254	91063499	91063254	ID=MsG0380017038.01.T01:cds;Parent=MsG0380017038.01.T01

Gene Sequence	>MsG0380017038.01.T01 ATGAGGAAGAAAAGAAAAGGAAGTGAAACTGATGCATCTACGGATCTGGGTGTTGTTAATTTGGATTTAGATCACGCTGGTTCTGTTGGATCGTCCAATTTGAAGTCTCATTACTCTTTAGAAGATTGTTGTAGGTTGAAGAAACGATGTAAAGAAGATGCTGACACTCACATTGAATTTGAAGGTTCCTTTAAAAGTAGGCTTGCTGGCATTGCTACTGCTCCGCCTTTGGGACGTCGTCTTTGA
Protein sequence	>MsG0380017038.01.T01 MRKKRKGSETDASTDLGVVNLDLDHAGSVGSSNLKSHYSLEDCCRLKKRCKEDADTHIEFEGSFKSRLAGIATAPPLGRRL*