Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028346.01.T01 | XP_003615412.1 | 93.53 | 541 | 28 | 2 | 1 | 534 | 1 | 541 | 0 | 1009 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0580028346.01 | 0.803782 | 2.958267e-49 | 1.499781e-46 |
| MsG0080048383.01 | MsG0580028346.01 | 0.839750 | 1.314184e-57 | 1.832221e-54 |
| MsG0080048765.01 | MsG0580028346.01 | 0.820661 | 6.096637e-53 | 4.853467e-50 |
| MsG0180000001.01 | MsG0580028346.01 | 0.833244 | 5.961943e-56 | 6.826112e-53 |
| MsG0180000101.01 | MsG0580028346.01 | 0.858511 | 7.833512e-63 | 2.004176e-59 |
| MsG0180000144.01 | MsG0580028346.01 | 0.810420 | 1.162863e-50 | 7.005987e-48 |
| MsG0180000235.01 | MsG0580028346.01 | 0.801581 | 8.414863e-49 | 4.033067e-46 |
| MsG0180000494.01 | MsG0580028346.01 | 0.833186 | 6.162048e-56 | 7.043226e-53 |
| MsG0180000555.01 | MsG0580028346.01 | 0.816296 | 5.950909e-52 | 4.200558e-49 |
| MsG0180000580.01 | MsG0580028346.01 | 0.819734 | 9.941330e-53 | 7.713621e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028346.01.T01 | MTR_5g067670 | 93.530 | 541 | 28 | 2 | 1 | 534 | 1 | 541 | 0.0 | 1009 |
| MsG0580028346.01.T01 | MTR_8g005790 | 61.133 | 512 | 162 | 10 | 37 | 534 | 26 | 514 | 0.0 | 576 |
| MsG0580028346.01.T01 | MTR_8g021310 | 47.628 | 506 | 235 | 8 | 39 | 534 | 20 | 505 | 8.62e-159 | 462 |
| MsG0580028346.01.T01 | MTR_2g007740 | 49.482 | 483 | 228 | 5 | 39 | 512 | 23 | 498 | 5.87e-157 | 457 |
| MsG0580028346.01.T01 | MTR_8g005485 | 55.134 | 448 | 141 | 8 | 37 | 472 | 26 | 425 | 9.34e-156 | 451 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028346.01.T01 | AT1G61670 | 61.047 | 516 | 176 | 4 | 28 | 534 | 14 | 513 | 0.0 | 619 |
| MsG0580028346.01.T01 | AT1G61670 | 61.047 | 516 | 176 | 4 | 28 | 534 | 14 | 513 | 0.0 | 619 |
| MsG0580028346.01.T01 | AT1G72480 | 46.498 | 514 | 238 | 8 | 37 | 534 | 17 | 509 | 2.35e-160 | 466 |
| MsG0580028346.01.T01 | AT2G01070 | 45.957 | 507 | 232 | 7 | 36 | 534 | 24 | 496 | 7.92e-152 | 444 |
| MsG0580028346.01.T01 | AT2G01070 | 48.098 | 447 | 205 | 4 | 36 | 474 | 24 | 451 | 5.67e-143 | 420 |
| MsG0580028346.01.T01 | AT1G10980 | 45.591 | 465 | 230 | 9 | 25 | 473 | 8 | 465 | 1.11e-134 | 401 |
Find 151 sgRNAs with CRISPR-Local
Find 325 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTCCTTCTTCTTGGTG+TGG | 0.525085 | 5:-78761300 | MsG0580028346.01.T01:CDS |
| AAAGAAGGTTCTTGTAAGTT+AGG | 0.306234 | 5:-78761875 | MsG0580028346.01.T01:CDS |
| AAAGATAGACTATACCTGGT+TGG | 0.603212 | 5:+78759953 | None:intergenic |
| AAATCCGGATGGTTATCTAC+CGG | 0.552193 | 5:-78761654 | MsG0580028346.01.T01:CDS |
| AACACGGCGGAGATTGAAGA+GGG | 0.582857 | 5:+78763258 | None:intergenic |
| AAGAACTGTGTGGAGAAATC+CGG | 0.522773 | 5:-78761669 | MsG0580028346.01.T01:CDS |
| AAGAGGGGTGGTTCCGGTGA+CGG | 0.529790 | 5:+78763274 | None:intergenic |
| AAGATCTAGAGGAAGACAAG+AGG | 0.616120 | 5:-78758988 | MsG0580028346.01.T01:CDS |
| AATACACCACACCAAGAAGA+AGG | 0.543762 | 5:+78761294 | None:intergenic |
| AATCCGGATGGTTATCTACC+GGG | 0.567234 | 5:-78761653 | MsG0580028346.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAAGGTTCTTGTAAGTT+AGG | - | Chr5:78760088-78760107 | MsG0580028346.01.T01:CDS | 30.0% | |
| AAAGGAATTTCAAATTACCG+CGG | - | Chr5:78762610-78762629 | MsG0580028346.01.T01:intron | 30.0% | |
| AACAAATCGCAATTACACTA+TGG | + | Chr5:78762408-78762427 | None:intergenic | 30.0% | |
| AAGCATTTGTGAAACTGTTT+GGG | + | Chr5:78761298-78761317 | None:intergenic | 30.0% | |
| AATAAGCTGAAAACAGCTTA+CGG | - | Chr5:78761243-78761262 | MsG0580028346.01.T01:CDS | 30.0% | |
| AATATACTCCATGAGATTTG+AGG | - | Chr5:78762647-78762666 | MsG0580028346.01.T01:intron | 30.0% | |
| ACAAAATGAAGAGACTAACA+TGG | - | Chr5:78760180-78760199 | MsG0580028346.01.T01:intron | 30.0% | |
| ACATGTCACAGTAACAATTA+TGG | + | Chr5:78761019-78761038 | None:intergenic | 30.0% | |
| ACTGAAGAATACATTTCTGT+AGG | + | Chr5:78761795-78761814 | None:intergenic | 30.0% | |
| AGAAACTTTAAACAAGTAGG+GGG | - | Chr5:78759730-78759749 | MsG0580028346.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 78758610 | 78763375 | 78758610 | ID=MsG0580028346.01;Name=MsG0580028346.01 |
| Chr5 | mRNA | 78758610 | 78763375 | 78758610 | ID=MsG0580028346.01.T01;Parent=MsG0580028346.01;Name=MsG0580028346.01.T01;_AED=0.35;_eAED=0.35;_QI=44|0.66|0.5|1|0.66|0.5|4|372|534 |
| Chr5 | exon | 78758610 | 78759177 | 78758610 | ID=MsG0580028346.01.T01:exon:46726;Parent=MsG0580028346.01.T01 |
| Chr5 | exon | 78759968 | 78760171 | 78759968 | ID=MsG0580028346.01.T01:exon:46725;Parent=MsG0580028346.01.T01 |
| Chr5 | exon | 78761125 | 78762066 | 78761125 | ID=MsG0580028346.01.T01:exon:46724;Parent=MsG0580028346.01.T01 |
| Chr5 | exon | 78763069 | 78763375 | 78763069 | ID=MsG0580028346.01.T01:exon:46723;Parent=MsG0580028346.01.T01 |
| Chr5 | five_prime_UTR | 78763332 | 78763375 | 78763332 | ID=MsG0580028346.01.T01:five_prime_utr;Parent=MsG0580028346.01.T01 |
| Chr5 | CDS | 78763069 | 78763331 | 78763069 | ID=MsG0580028346.01.T01:cds;Parent=MsG0580028346.01.T01 |
| Chr5 | CDS | 78761125 | 78762066 | 78761125 | ID=MsG0580028346.01.T01:cds;Parent=MsG0580028346.01.T01 |
| Chr5 | CDS | 78759968 | 78760171 | 78759968 | ID=MsG0580028346.01.T01:cds;Parent=MsG0580028346.01.T01 |
| Chr5 | CDS | 78758982 | 78759177 | 78758982 | ID=MsG0580028346.01.T01:cds;Parent=MsG0580028346.01.T01 |
| Chr5 | three_prime_UTR | 78758610 | 78758981 | 78758610 | ID=MsG0580028346.01.T01:three_prime_utr;Parent=MsG0580028346.01.T01 |
| Gene Sequence |
>MsG0580028346.01.T01 ATGTCTTACACCGATCACGGCGGCGATCGCCGTCACCGTCACCGGAACCACCCCTCTTCAATCTCCGCCGTGTTCCTCTACCTCACTTTCCATTCAATCATCACCCTCGTCGGTGCTTCCATTCACGACTACCAAAACGAAACGTTCATCCGTCGTGCTAATTCGTTTTTCTTTCATGGTGGTAGTGAAGGTCTTTACGCTTCTAAACCTATTGAGTTTAACCATTCTCTGGATAACTTTCCCACTGGTAAATCCTTCATCAGGTTTGAGTCTATCAACTTCCGGAGGACAAAAGAGTCTGCTCAAAAGGCAAGTTCGATGCAACAGAAAACAGGGTTGGTTGAAGCTATAATAATTAAGGTGAGAGATAGGAATAACATTGGGGGTGTTTATTTGAACTCTGATGCTATATGCTGCACACCAGAGCTTGCTAAAGAAGGTTCTTGTAAGTTAGGAGAGGTTATCATCCGTGAGAACCCGGATGAACCGAACGGGCCGAAACGCTTGCAAACGTTTTTTGAAGGACAAAATGAAGAGACTAACATGGTCATTCAAACTGTTGATATTAATAGTACTGGGATGTATTATTTGTATTTCATGTTTTGTGATCCTGAACTGAAGGACACGGTGATTTCTGGAAGAACTGTGTGGAGAAATCCGGATGGTTATCTACCGGGGAAGATGATGCCATTGATGACTTTTTATGGTTTGATGTCTTTGGCTTACCTTTTTCTTGGTCTTGTATGGTTTCTCTGGTTTGTAAAATATTGGAAAGATGTTATACAGTTGCATTACCACATTACTGCTGTTATTGGCCTAGGCATGTGTGAAATGGCTCTGTGGTATTTTGAGTATGCAAATTTCAATTCGACAGGAAGCAGGCCAATGGTAATTACAGTATGGGCCGTGACTTTCACTGCTGTCAAGAAGACAGTGTCACGGCTTCTTCTTTTGGTGGTTTCTATGGGCTATGGAGTTGTCCGTCCAACACTTGGTGGTCTAACCTCAAAAGTCCTTCTTCTTGGTGTGGTGTATTTTGTGGCCTCAGAAGCACTTGAGCTGGTGGAACATCTCGGTAATATTAATGACTTCTCTGGAAAAACAAGGCTTTTCCTGGTGCTGCCTGTGGCTCTGTTGGATGCTTGCTTTATTCTTTGGATCTTTTCATCATTATCTAAAACCCTTGAGAAGCTTCAGGTAACCTATATCTTGATTTTGTGTTTTATGCTAATAACTACAAGTGTCTCAGGCTCTGATTTAATCATGTTTCAGTTGTACTTCAATGCATCAGACCCTTTAGGGGAACTGTGGCGAAGAGCATGGGCCATCCCAGCTTTCTGGACTTTGCTTGCTTATGCTCTCCTGATAGTGATCTGCATTCTCTGGGCCCCATCTCGAAATCCAACCAGGTATTCGTACTCGGAAGAGACGGGAGATGATTTTGATGAGGAAGCTGTCGCAGTTGTTGGAAGTGGAGTTAAGATGTCTGGAGAAATGTCTACCATGCTAGAAAGAAAGGATAGAAAGGCATCGTCTACATCACTTGCTACAGATCATCATGTATTTGGAGTTATTGAAGATCTAGAGGAAGACAAGAGGGAGTGA |
| Protein sequence |
>MsG0580028346.01.T01 MSYTDHGGDRRHRHRNHPSSISAVFLYLTFHSIITLVGASIHDYQNETFIRRANSFFFHGGSEGLYASKPIEFNHSLDNFPTGKSFIRFESINFRRTKESAQKASSMQQKTGLVEAIIIKVRDRNNIGGVYLNSDAICCTPELAKEGSCKLGEVIIRENPDEPNGPKRLQTFFEGQNEETNMVIQTVDINSTGMYYLYFMFCDPELKDTVISGRTVWRNPDGYLPGKMMPLMTFYGLMSLAYLFLGLVWFLWFVKYWKDVIQLHYHITAVIGLGMCEMALWYFEYANFNSTGSRPMVITVWAVTFTAVKKTVSRLLLLVVSMGYGVVRPTLGGLTSKVLLLGVVYFVASEALELVEHLGNINDFSGKTRLFLVLPVALLDACFILWIFSSLSKTLEKLQVTYILILCFMLITTSVSGSDLIMFQLYFNASDPLGELWRRAWAIPAFWTLLAYALLIVICILWAPSRNPTRYSYSEETGDDFDEEAVAVVGSGVKMSGEMSTMLERKDRKASSTSLATDHHVFGVIEDLEEDKRE* |
