AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017067.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017067.01.T01 MTR_3g100330 92.188 128 9 1 1 127 1 128 1.72e-79 234
MsG0380017067.01.T01 MTR_5g066100 67.969 128 37 2 1 127 2 126 5.17e-49 160
MsG0380017067.01.T01 MTR_4g093930 44.800 125 66 2 4 127 5 127 1.71e-30 114
MsG0380017067.01.T01 MTR_2g009520 41.803 122 67 2 3 123 4 122 1.67e-26 103
MsG0380017067.01.T01 MTR_2g020420 44.715 123 66 2 1 122 1 122 1.07e-22 92.4
MsG0380017067.01.T01 MTR_2g020420 44.715 123 66 2 1 122 1 122 1.25e-22 92.4
MsG0380017067.01.T01 MTR_4g118660 41.463 123 68 3 1 122 42 161 2.02e-20 85.9
MsG0380017067.01.T01 MTR_6g059870 37.398 123 72 3 1 122 1 119 6.10e-17 75.9
MsG0380017067.01.T01 MTR_5g097170 35.938 128 77 3 1 127 1 124 1.69e-16 74.7
MsG0380017067.01.T01 MTR_7g114150 37.500 128 76 3 1 127 1 125 5.96e-16 73.2
MsG0380017067.01.T01 MTR_3g091040 37.398 123 72 3 1 122 1 119 3.15e-15 71.2
MsG0380017067.01.T01 MTR_1g081970 37.984 129 74 4 1 127 1 125 2.83e-14 68.6
MsG0380017067.01.T01 MTR_1g060440 37.500 128 76 3 1 127 1 125 1.01e-13 67.0
MsG0380017067.01.T01 MTR_3g082810 33.607 122 76 3 1 121 1 118 1.79e-13 66.2
MsG0380017067.01.T01 MTR_1g081960 37.288 118 68 4 1 116 51 164 3.60e-12 62.0
MsG0380017067.01.T01 MTR_2g014480 49.153 59 26 2 68 123 3 60 6.27e-11 58.5
MsG0380017067.01.T01 MTR_4g015840 50.847 59 25 2 68 123 3 60 7.69e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017067.01.T01 AT2G01710 56.154 130 52 3 2 127 5 133 9.26e-37 127
MsG0380017067.01.T01 AT2G01710 56.154 130 52 3 2 127 49 177 1.87e-36 127
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 3.55e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT5G64360 44.355 124 64 4 7 127 17 138 4.14e-20 85.1
MsG0380017067.01.T01 AT4G19570 40.310 129 72 3 1 128 1 125 1.59e-19 83.6
MsG0380017067.01.T01 AT4G19580 40.650 123 68 3 1 122 1 119 1.89e-19 82.0
MsG0380017067.01.T01 AT5G09540 42.063 126 68 4 5 127 11 134 8.63e-19 79.7
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G37380 40.650 123 68 3 1 122 1 119 1.60e-18 80.5
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 3.30e-18 79.7
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 7.79e-18 78.6
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 7.79e-18 78.6
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 7.79e-18 78.6
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 8.06e-18 78.6
MsG0380017067.01.T01 AT5G53150 38.211 123 71 3 1 122 1 119 8.06e-18 78.6
MsG0380017067.01.T01 AT4G19590 38.400 125 73 3 1 124 1 122 1.26e-17 77.8
MsG0380017067.01.T01 AT4G19590 38.400 125 73 3 1 124 1 122 1.36e-17 77.4
MsG0380017067.01.T01 AT3G04980 40.625 128 71 3 1 127 1 124 6.07e-17 76.3
MsG0380017067.01.T01 AT3G04980 40.625 128 71 3 1 127 1 124 6.07e-17 76.3
MsG0380017067.01.T01 AT3G06340 36.585 123 74 3 1 122 1 120 2.29e-16 74.3
MsG0380017067.01.T01 AT3G06340 36.585 123 74 3 1 122 1 120 2.29e-16 74.3
MsG0380017067.01.T01 AT3G06340 36.585 123 74 3 1 122 1 120 2.29e-16 74.3
MsG0380017067.01.T01 AT3G06340 36.585 123 74 3 1 122 1 120 2.29e-16 74.3
MsG0380017067.01.T01 AT1G62970 39.167 120 70 3 7 123 17 136 2.52e-16 74.3
MsG0380017067.01.T01 AT2G05230 34.109 129 80 3 1 128 1 125 1.05e-15 72.4
MsG0380017067.01.T01 AT2G05250 34.109 129 80 3 1 128 1 125 1.05e-15 72.4
MsG0380017067.01.T01 AT4G19590 37.719 114 67 3 12 124 1 111 3.34e-15 70.9
MsG0380017067.01.T01 AT5G27240 41.129 124 66 4 1 122 1 119 1.35e-14 69.3
MsG0380017067.01.T01 AT5G27240 41.129 124 66 4 1 122 1 119 1.35e-14 69.3
MsG0380017067.01.T01 AT5G27240 41.129 124 66 4 1 122 1 119 1.35e-14 69.3
MsG0380017067.01.T01 AT2G25560 35.772 123 74 3 1 122 1 119 1.43e-14 69.3
MsG0380017067.01.T01 AT3G04980 40.909 110 60 3 19 127 1 106 1.66e-13 66.2
MsG0380017067.01.T01 AT5G18750 36.885 122 71 4 3 122 2 119 4.64e-13 65.1
MsG0380017067.01.T01 AT5G37440 35.537 121 69 4 9 128 21 133 1.15e-11 60.5

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCGAAGCAGAACGGTTAC+TGG 0.326509 3:-91555794 MsG0380017067.01.T01:CDS
TTCCAGTAACCGTTCTGCTT+CGG 0.360483 3:+91555792 None:intergenic
CAAACTCGTTAAGGATGCTT+GGG 0.376604 3:-91555479 MsG0380017067.01.T01:CDS
GTAAGAAACGGATTCTTATC+AGG 0.393315 3:+91555514 None:intergenic
GACTCGCTTCAAACTCGTTA+AGG 0.423383 3:-91555488 MsG0380017067.01.T01:CDS
GAAACCGAACCTCTTCTTGA+AGG 0.424732 3:-91555703 MsG0380017067.01.T01:CDS
CCTGTGAATTGCGATCAATT+TGG 0.429303 3:+91555587 None:intergenic
TCAAACTCGTTAAGGATGCT+TGG 0.443507 3:-91555480 MsG0380017067.01.T01:CDS
GGAACCTTCAAGAAGAGGTT+CGG 0.467349 3:+91555699 None:intergenic
GCAGAACGGTTACTGGAAAT+CGG 0.494239 3:-91555787 MsG0380017067.01.T01:CDS
CCAAATTGATCGCAATTCAC+AGG 0.501908 3:-91555587 MsG0380017067.01.T01:CDS
AAGCGAGTCAGCGTAAGAAA+CGG 0.502766 3:+91555502 None:intergenic
TGCTATGTTGAGTTGTTGAC+CGG 0.508926 3:+91555442 None:intergenic
TGGTGGTTGTTGAGATTGAG+AGG 0.510306 3:+91555631 None:intergenic
GAAGAAGAAGAGCGAGAGTA+CGG 0.517568 3:+91555539 None:intergenic
TCAACAACCACCACCTCGAT+TGG 0.523308 3:-91555621 MsG0380017067.01.T01:CDS
CGGCGTACCAATCGAGGTGG+TGG 0.534499 3:+91555614 None:intergenic
GGAGAACGGCGTACCAATCG+AGG 0.566993 3:+91555608 None:intergenic
TGATCGGAACCTTCAAGAAG+AGG 0.608536 3:+91555694 None:intergenic
ATTGCGATCAATTTGGAGAA+CGG 0.611753 3:+91555594 None:intergenic
AACAATAGCGAGGATCTGAT+CGG 0.616293 3:+91555678 None:intergenic
TGTTGATGTTCTCGAAGCTG+CGG 0.616667 3:-91555659 MsG0380017067.01.T01:CDS
AAACTCGTTAAGGATGCTTG+GGG 0.642473 3:-91555478 MsG0380017067.01.T01:CDS
CAAGCAAAGCCGAAGCAGAA+CGG 0.656161 3:-91555801 MsG0380017067.01.T01:CDS
CGAGAACATCAACAATAGCG+AGG 0.683416 3:+91555668 None:intergenic
GAACGGCGTACCAATCGAGG+TGG 0.714852 3:+91555611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTACAAAAACGAGATCTCAA+AGG - Chr3:91555498-91555517 MsG0380017067.01.T01:CDS 30.0%
ATTGCGATCAATTTGGAGAA+CGG + Chr3:91555661-91555680 None:intergenic 35.0%
! GTAAGAAACGGATTCTTATC+AGG + Chr3:91555741-91555760 None:intergenic 35.0%
AAACTCGTTAAGGATGCTTG+GGG - Chr3:91555774-91555793 MsG0380017067.01.T01:CDS 40.0%
AACAATAGCGAGGATCTGAT+CGG + Chr3:91555577-91555596 None:intergenic 40.0%
CAAACTCGTTAAGGATGCTT+GGG - Chr3:91555773-91555792 MsG0380017067.01.T01:CDS 40.0%
CCAAATTGATCGCAATTCAC+AGG - Chr3:91555665-91555684 MsG0380017067.01.T01:CDS 40.0%
CCTGTGAATTGCGATCAATT+TGG + Chr3:91555668-91555687 None:intergenic 40.0%
TCAAACTCGTTAAGGATGCT+TGG - Chr3:91555772-91555791 MsG0380017067.01.T01:CDS 40.0%
AAGCGAGTCAGCGTAAGAAA+CGG + Chr3:91555753-91555772 None:intergenic 45.0%
CGAGAACATCAACAATAGCG+AGG + Chr3:91555587-91555606 None:intergenic 45.0%
GAAACCGAACCTCTTCTTGA+AGG - Chr3:91555549-91555568 MsG0380017067.01.T01:CDS 45.0%
GAAGAAGAAGAGCGAGAGTA+CGG + Chr3:91555716-91555735 None:intergenic 45.0%
GACTCGCTTCAAACTCGTTA+AGG - Chr3:91555764-91555783 MsG0380017067.01.T01:CDS 45.0%
GCAGAACGGTTACTGGAAAT+CGG - Chr3:91555465-91555484 MsG0380017067.01.T01:CDS 45.0%
GGAACCTTCAAGAAGAGGTT+CGG + Chr3:91555556-91555575 None:intergenic 45.0%
TGATCGGAACCTTCAAGAAG+AGG + Chr3:91555561-91555580 None:intergenic 45.0%
TGGTGGTTGTTGAGATTGAG+AGG + Chr3:91555624-91555643 None:intergenic 45.0%
TGTTGATGTTCTCGAAGCTG+CGG - Chr3:91555593-91555612 MsG0380017067.01.T01:CDS 45.0%
TTCCAGTAACCGTTCTGCTT+CGG + Chr3:91555463-91555482 None:intergenic 45.0%
!! TTGGGGTGTTTTGTCTGATC+CGG - Chr3:91555791-91555810 MsG0380017067.01.T01:CDS 45.0%
CAAGCAAAGCCGAAGCAGAA+CGG - Chr3:91555451-91555470 MsG0380017067.01.T01:CDS 50.0%
TCAACAACCACCACCTCGAT+TGG - Chr3:91555631-91555650 MsG0380017067.01.T01:CDS 50.0%
AGCCGAAGCAGAACGGTTAC+TGG - Chr3:91555458-91555477 MsG0380017067.01.T01:CDS 55.0%
GAACGGCGTACCAATCGAGG+TGG + Chr3:91555644-91555663 None:intergenic 60.0%
GGAGAACGGCGTACCAATCG+AGG + Chr3:91555647-91555666 None:intergenic 60.0%
CGGCGTACCAATCGAGGTGG+TGG + Chr3:91555641-91555660 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 91555444 91555830 91555444 ID=MsG0380017067.01;Name=MsG0380017067.01
Chr3 mRNA 91555444 91555830 91555444 ID=MsG0380017067.01.T01;Parent=MsG0380017067.01;Name=MsG0380017067.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|128
Chr3 exon 91555444 91555830 91555444 ID=MsG0380017067.01.T01:exon:20697;Parent=MsG0380017067.01.T01
Chr3 CDS 91555444 91555830 91555444 ID=MsG0380017067.01.T01:cds;Parent=MsG0380017067.01.T01
Gene Sequence

>MsG0380017067.01.T01

ATGAACACAAGCAAAGCCGAAGCAGAACGGTTACTGGAAATCGGAGAAGAGCTCTTACAAAAACGAGATCTCAAAGGTTCAAGAGAAATTGCGAATCTTGTTCAAGAAACCGAACCTCTTCTTGAAGGTTCCGATCAGATCCTCGCTATTGTTGATGTTCTCGAAGCTGCGGAAAAACCTCTCAATCTCAACAACCACCACCTCGATTGGTACGCCGTTCTCCAAATTGATCGCAATTCACAGGATCTCAATCGCATTAAGAAACAGTACCGTACTCTCGCTCTTCTTCTTCATCCTGATAAGAATCCGTTTCTTACGCTGACTCGCTTCAAACTCGTTAAGGATGCTTGGGGTGTTTTGTCTGATCCGGTCAACAACTCAACATAG

Protein sequence

>MsG0380017067.01.T01

MNTSKAEAERLLEIGEELLQKRDLKGSREIANLVQETEPLLEGSDQILAIVDVLEAAEKPLNLNNHHLDWYAVLQIDRNSQDLNRIKKQYRTLALLLHPDKNPFLTLTRFKLVKDAWGVLSDPVNNST*