AlfalfaGEDB | MsG0480018322.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018322.01

KEGG: -
GO: -
PFAM: PF05678

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018322.01.T01	XP_039688797.1	88.652	141	2	3	1	139	1	129	2.94E-71	222

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018322.01.T01	Q9M8L3	35.503	169	43	9	4	151	7	130	1.95E-11	62

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018322.01.T01	G7JGW0	88.652	141	2	3	1	139	1	129	1.40e-71	222

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048948.01	MsG0480018322.01	-0.832661	8.321882e-56	9.363948e-53
MsG0080049038.01	MsG0480018322.01	-0.826375	2.807840e-54	2.628701e-51
MsG0080049066.01	MsG0480018322.01	-0.809362	1.964220e-50	1.150983e-47
MsG0180003779.01	MsG0480018322.01	-0.830244	3.275327e-55	3.431672e-52
MsG0180003908.01	MsG0480018322.01	-0.804332	2.272807e-49	1.168659e-46
MsG0180004226.01	MsG0480018322.01	-0.804023	2.635020e-49	1.344272e-46
MsG0180004491.01	MsG0480018322.01	0.813393	2.618998e-51	1.708295e-48
MsG0180005817.01	MsG0480018322.01	-0.831115	2.004691e-55	2.154578e-52
MsG0180005848.01	MsG0480018322.01	-0.809784	1.594275e-50	9.447323e-48
MsG0280011200.01	MsG0480018322.01	-0.814793	1.285491e-51	8.708526e-49
MsG0280011299.01	MsG0480018322.01	-0.805552	1.263089e-49	6.701922e-47
MsG0280011301.01	MsG0480018322.01	-0.801216	9.996737e-49	4.747462e-46
MsG0380012281.01	MsG0480018322.01	-0.822064	2.892667e-53	2.395268e-50
MsG0380014410.01	MsG0480018322.01	0.809354	1.972536e-50	1.155604e-47
MsG0380014642.01	MsG0480018322.01	-0.807604	4.661112e-50	2.607255e-47
MsG0380015752.01	MsG0480018322.01	-0.804636	1.964618e-49	1.018117e-46
MsG0380016931.01	MsG0480018322.01	-0.812952	3.272461e-51	2.108953e-48
MsG0380016934.01	MsG0480018322.01	-0.806918	6.514382e-50	3.579713e-47
MsG0380016935.01	MsG0480018322.01	-0.802518	5.400004e-49	2.650510e-46
MsG0380017667.01	MsG0480018322.01	0.811675	6.218738e-51	3.873265e-48
MsG0380017962.01	MsG0480018322.01	-0.800415	1.456756e-48	6.779284e-46
MsG0380018072.01	MsG0480018322.01	-0.800425	1.449888e-48	6.749107e-46
MsG0480018112.01	MsG0480018322.01	-0.804118	2.518468e-49	1.287914e-46
MsG0480018322.01	MsG0480019588.01	0.813230	2.843285e-51	1.846203e-48
MsG0480018322.01	MsG0480020841.01	-0.810671	1.026720e-50	6.227674e-48
MsG0480018322.01	MsG0480020842.01	-0.814760	1.307149e-51	8.847167e-49
MsG0480018322.01	MsG0480020938.01	-0.823917	1.070017e-53	9.339006e-51
MsG0480018322.01	MsG0480020958.01	0.803071	4.151769e-49	2.066701e-46
MsG0480018322.01	MsG0480021912.01	-0.828266	9.893194e-55	9.783626e-52
MsG0480018322.01	MsG0480023749.01	-0.817260	3.615419e-52	2.620064e-49
MsG0480018322.01	MsG0580024493.01	-0.807877	4.077028e-50	2.296886e-47
MsG0480018322.01	MsG0580024678.01	-0.805777	1.133369e-49	6.048808e-47
MsG0480018322.01	MsG0580025117.01	0.817712	2.860476e-52	2.098861e-49
MsG0480018322.01	MsG0580029177.01	-0.802798	4.728272e-49	2.337582e-46
MsG0480018322.01	MsG0580029623.01	0.819145	1.354605e-52	1.033896e-49
MsG0480018322.01	MsG0580030019.01	0.828265	9.898582e-55	9.788683e-52
MsG0480018322.01	MsG0680030635.01	-0.806750	7.067706e-50	3.866941e-47
MsG0480018322.01	MsG0780037751.01	0.808670	2.763704e-50	1.589758e-47
MsG0480018322.01	MsG0780039821.01	-0.808284	3.339945e-50	1.902062e-47
MsG0480018322.01	MsG0780039918.01	0.805456	1.323426e-49	7.005005e-47
MsG0480018322.01	MsG0780040072.01	-0.807735	4.371266e-50	2.453663e-47
MsG0480018322.01	MsG0780040121.01	-0.804262	2.350996e-49	1.206656e-46
MsG0480018322.01	MsG0780040685.01	-0.806516	7.920172e-50	4.307442e-47
MsG0480018322.01	MsG0780041100.01	0.824772	6.734073e-54	6.023069e-51
MsG0480018322.01	MsG0780041106.01	-0.816753	4.700768e-52	3.359762e-49
MsG0480018322.01	MsG0780041172.01	0.824485	7.869647e-54	6.979845e-51
MsG0480018322.01	MsG0780041720.01	-0.817132	3.864100e-52	2.790551e-49
MsG0480018322.01	MsG0780041783.01	-0.804920	1.713589e-49	8.946688e-47
MsG0480018322.01	MsG0880045703.01	-0.800652	1.303545e-48	6.102772e-46
MsG0480018322.01	MsG0880045804.01	0.807542	4.803393e-50	2.682623e-47
MsG0480018322.01	MsG0880046838.01	-0.852034	6.013789e-61	1.240548e-57
MsG0480018322.01	MsG0880047318.01	-0.809872	1.526821e-50	9.068002e-48
MsG0480018322.01	MsG0880047493.01	0.817760	2.789467e-52	2.049536e-49
MsG0280007786.01	MsG0480018322.01	0.803793	2.942187e-49	1.492093e-46
MsG0280009742.01	MsG0480018322.01	-0.800520	1.387038e-48	6.471773e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018322.01.T01	MTR_4g009950	88.652	141	2	3	1	139	1	129	3.56e-75	222

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018322.01.T01	AT1G80450	35.503	169	43	9	4	151	7	130	1.99e-12	62.0

Find 36 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGTGGAGGACGGTGGGCT+TGG	0.256659	4:-3348837	None:intergenic
GAGGACGGTGGGCTTGGGCT+TGG	0.257828	4:-3348831	None:intergenic
CAACATGCGAAGTGTTATTA+TGG	0.268568	4:+3349023	MsG0480018322.01.T01:CDS
TCATGTTATTGTTGTTGTTT+TGG	0.291134	4:-3348969	None:intergenic
AGGACGGTGGGCTTGGGCTT+GGG	0.306796	4:-3348830	None:intergenic
TAATATTCCAATGGTGAAAC+TGG	0.334222	4:-3349051	None:intergenic
TGGTGGAGGACGGTGGGCTT+GGG	0.338898	4:-3348836	None:intergenic
TTGTGTTTGGTTGTGTTGTT+AGG	0.343814	4:-3348693	None:intergenic
GGTTCCATGGTGGTTGTTGT+TGG	0.392587	4:-3348856	None:intergenic
TCCATGGTGGTTGTTGTTGG+TGG	0.405900	4:-3348853	None:intergenic
GCTTGTACAAAGGTTGTGTT+TGG	0.410037	4:-3348706	None:intergenic
ACAACTTGTCGGAAATTTGA+TGG	0.417823	4:-3348733	None:intergenic
GCGTTCGTGGAGTTTGAAGT+TGG	0.435488	4:-3348899	None:intergenic
CGTTCGTGGAGTTTGAAGTT+GGG	0.446220	4:-3348898	None:intergenic
TGGTTGTTGTTGGTGGAGGA+CGG	0.455302	4:-3348846	None:intergenic
TAAGCCCATTGGTGGTTCCA+TGG	0.464436	4:-3348869	None:intergenic
ACCATGCATTCACCAACATA+TGG	0.465787	4:+3348664	None:intergenic
GGCTTCACCAGTTTCACCAT+TGG	0.477870	4:+3349044	MsG0480018322.01.T01:CDS
TGTTGTTGGTGGAGGACGGT+GGG	0.481918	4:-3348842	None:intergenic
AAAACTCCAACTCAACGTTT+CGG	0.499017	4:+3348930	MsG0480018322.01.T01:CDS
AGACGAGAAGGGAGTGTGAG+TGG	0.503781	4:-3348802	None:intergenic
TGATGGGTCTTGTGATGCTC+CGG	0.504118	4:-3348767	None:intergenic
ATGGTGGTTGTTGTTGGTGG+AGG	0.515633	4:-3348850	None:intergenic
AACCACCATGGAACCACCAA+TGG	0.516253	4:+3348864	MsG0480018322.01.T01:CDS
TGATGGGTCAGCTTGTACAA+AGG	0.525050	4:-3348716	None:intergenic
ACCACCATGGAACCACCAAT+GGG	0.535319	4:+3348865	MsG0480018322.01.T01:CDS
GTTTGGTTGTGTTGTTAGGT+CGG	0.535973	4:-3348689	None:intergenic
GCTTGGGCTGTTAGACGAGA+AGG	0.539771	4:-3348814	None:intergenic
TTGTTGTTGGTGGAGGACGG+TGG	0.540140	4:-3348843	None:intergenic
CAACTTGTCGGAAATTTGAT+GGG	0.565093	4:-3348732	None:intergenic
GGACTATAGTAATATTCCAA+TGG	0.579476	4:-3349060	None:intergenic
GTTAGGTCGGATCCATATGT+TGG	0.606638	4:-3348676	None:intergenic
TCCATATGTTGGTGAATGCA+TGG	0.614670	4:-3348665	None:intergenic
CTTGGGCTGTTAGACGAGAA+GGG	0.624849	4:-3348813	None:intergenic
TCCACCAACAACAACCACCA+TGG	0.648779	4:+3348852	MsG0480018322.01.T01:CDS
GCCCATTGGTGGTTCCATGG+TGG	0.657294	4:-3348866	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTTTTCTTCATTTTCATG+TGG	-	Chr4:3349092-3349111	None:intergenic	25.0%
!!!	TCATGTTATTGTTGTTGTTT+TGG	-	Chr4:3348972-3348991	None:intergenic	25.0%
	GGACTATAGTAATATTCCAA+TGG	-	Chr4:3349063-3349082	None:intergenic	30.0%
	TAATATTCCAATGGTGAAAC+TGG	-	Chr4:3349054-3349073	None:intergenic	30.0%
!	TTCATTTTCATGTGGTGATT+TGG	-	Chr4:3349084-3349103	None:intergenic	30.0%
!!	TTAGTTTTTGGACAACTTGT+CGG	-	Chr4:3348747-3348766	None:intergenic	30.0%
!!!	TTGTTGTTTTGGAACATCAT+GGG	-	Chr4:3348961-3348980	None:intergenic	30.0%
	AAAACTCCAACTCAACGTTT+CGG	+	Chr4:3348930-3348949	MsG0480018322.01.T01:CDS	35.0%
	ACAACTTGTCGGAAATTTGA+TGG	-	Chr4:3348736-3348755	None:intergenic	35.0%
	CAACTTGTCGGAAATTTGAT+GGG	-	Chr4:3348735-3348754	None:intergenic	35.0%
	CAAGTTGTCCAAAAACTAAC+CGG	+	Chr4:3348748-3348767	MsG0480018322.01.T01:CDS	35.0%
!	CAACATGCGAAGTGTTATTA+TGG	+	Chr4:3349023-3349042	MsG0480018322.01.T01:CDS	35.0%
!	TTGTGTTTGGTTGTGTTGTT+AGG	-	Chr4:3348696-3348715	None:intergenic	35.0%
!!!	GTTGTTGTTTTGGAACATCA+TGG	-	Chr4:3348962-3348981	None:intergenic	35.0%
!!!	TGAGTTGGAGTTTTTTAGAG+CGG	-	Chr4:3348924-3348943	None:intergenic	35.0%
!!!	TTGGGTTTTTTTAAGCCCAT+TGG	-	Chr4:3348883-3348902	None:intergenic	35.0%
	GCTTGTACAAAGGTTGTGTT+TGG	-	Chr4:3348709-3348728	None:intergenic	40.0%
!	GAGTGTGAGTGGAAGTTTTT+GGG	-	Chr4:3348794-3348813	None:intergenic	40.0%
!	GATTTTCCGAAACGTTGAGT+TGG	-	Chr4:3348939-3348958	None:intergenic	40.0%
!	GTTTGGTTGTGTTGTTAGGT+CGG	-	Chr4:3348692-3348711	None:intergenic	40.0%
!!!	GGTTTTTTTAAGCCCATTGG+TGG	-	Chr4:3348880-3348899	None:intergenic	40.0%
	GTTAGGTCGGATCCATATGT+TGG	-	Chr4:3348679-3348698	None:intergenic	45.0%
!	CGTTCGTGGAGTTTGAAGTT+GGG	-	Chr4:3348901-3348920	None:intergenic	45.0%
!	GGAGTGTGAGTGGAAGTTTT+TGG	-	Chr4:3348795-3348814	None:intergenic	45.0%
!	TGATGGGTCAGCTTGTACAA+AGG	-	Chr4:3348719-3348738	None:intergenic	45.0%
!!	AGTGGAAGTTTTTGGGCTGA+TGG	-	Chr4:3348787-3348806	None:intergenic	45.0%
!!	GTGGAAGTTTTTGGGCTGAT+GGG	-	Chr4:3348786-3348805	None:intergenic	45.0%
!!!	GTGATGCTCCGGTTAGTTTT+TGG	-	Chr4:3348759-3348778	None:intergenic	45.0%
	AACCACCATGGAACCACCAA+TGG	+	Chr4:3348864-3348883	MsG0480018322.01.T01:CDS	50.0%
	ACCACCATGGAACCACCAAT+GGG	+	Chr4:3348865-3348884	MsG0480018322.01.T01:CDS	50.0%
	CTTGGGCTGTTAGACGAGAA+GGG	-	Chr4:3348816-3348835	None:intergenic	50.0%
	GGCTTCACCAGTTTCACCAT+TGG	+	Chr4:3349044-3349063	MsG0480018322.01.T01:CDS	50.0%
	GGTTCCATGGTGGTTGTTGT+TGG	-	Chr4:3348859-3348878	None:intergenic	50.0%
	TCCACCAACAACAACCACCA+TGG	+	Chr4:3348852-3348871	MsG0480018322.01.T01:CDS	50.0%
!	ATGGTGGTTGTTGTTGGTGG+AGG	-	Chr4:3348853-3348872	None:intergenic	50.0%
!	GCGTTCGTGGAGTTTGAAGT+TGG	-	Chr4:3348902-3348921	None:intergenic	50.0%
!	TAAGCCCATTGGTGGTTCCA+TGG	-	Chr4:3348872-3348891	None:intergenic	50.0%
!	TCCATGGTGGTTGTTGTTGG+TGG	-	Chr4:3348856-3348875	None:intergenic	50.0%
!	TGATGGGTCTTGTGATGCTC+CGG	-	Chr4:3348770-3348789	None:intergenic	50.0%
!	TGGTTGTTGTTGGTGGAGGA+CGG	-	Chr4:3348849-3348868	None:intergenic	50.0%
!!!	GTTTTTTAGAGCGGCGTTCG+TGG	-	Chr4:3348915-3348934	None:intergenic	50.0%
	GCTTGGGCTGTTAGACGAGA+AGG	-	Chr4:3348817-3348836	None:intergenic	55.0%
!	AGACGAGAAGGGAGTGTGAG+TGG	-	Chr4:3348805-3348824	None:intergenic	55.0%
!	TGTTGTTGGTGGAGGACGGT+GGG	-	Chr4:3348845-3348864	None:intergenic	55.0%
!	TTGTTGTTGGTGGAGGACGG+TGG	-	Chr4:3348846-3348865	None:intergenic	55.0%
!	GCCCATTGGTGGTTCCATGG+TGG	-	Chr4:3348869-3348888	None:intergenic	60.0%
!	AGGACGGTGGGCTTGGGCTT+GGG	-	Chr4:3348833-3348852	None:intergenic	65.0%
!	TGGTGGAGGACGGTGGGCTT+GGG	-	Chr4:3348839-3348858	None:intergenic	65.0%
!	TTGGTGGAGGACGGTGGGCT+TGG	-	Chr4:3348840-3348859	None:intergenic	65.0%
!	GAGGACGGTGGGCTTGGGCT+TGG	-	Chr4:3348834-3348853	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	3348667	3349125	3348667	ID=MsG0480018322.01;Name=MsG0480018322.01
Chr4	mRNA	3348667	3349125	3348667	ID=MsG0480018322.01.T01;Parent=MsG0480018322.01;Name=MsG0480018322.01.T01;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|152
Chr4	exon	3348667	3349125	3348667	ID=MsG0480018322.01.T01:exon:928;Parent=MsG0480018322.01.T01
Chr4	CDS	3348667	3349125	3348667	ID=MsG0480018322.01.T01:cds;Parent=MsG0480018322.01.T01

Gene Sequence

>MsG0480018322.01.T01

ATGCATTCACCAACATATGGATCCGACCTAACAACACAACCAAACACAACCTTTGTACAAGCTGACCCATCAAATTTCCGACAAGTTGTCCAAAAACTAACCGGAGCATCACAAGACCCATCAGCCCAAAAACTTCCACTCACACTCCCTTCTCGTCTAACAGCCCAAGCCCAAGCCCACCGTCCTCCACCAACAACAACCACCATGGAACCACCAATGGGCTTAAAAAAACCCAACTTCAAACTCCACGAACGCCGCTCTAAAAAACTCCAACTCAACGTTTCGGAAAATCCCATGATGTTCCAAAACAACAACAATAACATGATGATGATGTTAATGAATAGTAACAACAACAACAACATGCGAAGTGTTATTATGGCTTCACCAGTTTCACCATTGGAATATTACTATAGTCCAAATCACCACATGAAAATGAAGAAAAAGAAGAAGAAAAAGTGA

Protein sequence

>MsG0480018322.01.T01

MHSPTYGSDLTTQPNTTFVQADPSNFRQVVQKLTGASQDPSAQKLPLTLPSRLTAQAQAHRPPPTTTTMEPPMGLKKPNFKLHERRSKKLQLNVSENPMMFQNNNNNMMMMLMNSNNNNNMRSVIMASPVSPLEYYYSPNHHMKMKKKKKKK*