Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039821.01.T01 | XP_003624162.1 | 98.598 | 214 | 3 | 0 | 1 | 214 | 1 | 214 | 6.86E-150 | 425 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0780039821.01 | -0.846120 | 2.651335e-59 | 4.516480e-56 |
| MsG0080047910.01 | MsG0780039821.01 | 0.807641 | 4.577851e-50 | 2.563205e-47 |
| MsG0080047967.01 | MsG0780039821.01 | 0.934455 | 3.931328e-96 | 2.969913e-91 |
| MsG0080048036.01 | MsG0780039821.01 | -0.863555 | 2.289793e-64 | 6.969368e-61 |
| MsG0080048059.01 | MsG0780039821.01 | 0.928514 | 2.591190e-92 | 1.371506e-87 |
| MsG0080048111.01 | MsG0780039821.01 | -0.819492 | 1.128689e-52 | 8.699162e-50 |
| MsG0080048202.01 | MsG0780039821.01 | -0.804030 | 2.627118e-49 | 1.340426e-46 |
| MsG0080048347.01 | MsG0780039821.01 | -0.836195 | 1.078967e-56 | 1.350096e-53 |
| MsG0080048352.01 | MsG0780039821.01 | -0.829941 | 3.883948e-55 | 4.033828e-52 |
| MsG0080048486.01 | MsG0780039821.01 | -0.828297 | 9.723702e-55 | 9.624832e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039821.01.T01 | MTR_7g079900 | 98.598 | 214 | 3 | 0 | 1 | 214 | 1 | 214 | 8.31e-154 | 425 |
Find 89 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAAATTGCCTTCTAGA+AGG | 0.412684 | 7:+69435270 | None:intergenic |
| AATCAACAAAACTTAAGTCA+TGG | 0.592569 | 7:-69435391 | MsG0780039821.01.T01:exon |
| ACAAGTGGGCTTGATGTAAC+TGG | 0.422772 | 7:+69434573 | None:intergenic |
| ACACACACTGGTTGAATTGA+GGG | 0.479464 | 7:+69435443 | None:intergenic |
| ACCGAATATGCCGGATCAGC+AGG | 0.445265 | 7:-69434420 | MsG0780039821.01.T01:CDS |
| ACTTCAATTCCCCTAAGAAG+TGG | 0.571599 | 7:+69434504 | None:intergenic |
| AGAAGGCAATTGATGGAGTG+GGG | 0.661086 | 7:+69435287 | None:intergenic |
| AGACCTTTGGTCACACCTAG+GGG | 0.664459 | 7:-69435324 | MsG0780039821.01.T01:CDS |
| AGAGGCCCGGCTTTAACGAA+CGG | 0.558183 | 7:+69434450 | None:intergenic |
| AGAGGTACCATGCAACAAGT+GGG | 0.679084 | 7:+69434559 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTTAAATTGCCTTCTAGA+AGG | + | Chr7:69434228-69434247 | None:intergenic | 30.0% | |
| ATGAAACTCCATAGATTGTA+AGG | + | Chr7:69435143-69435162 | None:intergenic | 30.0% | |
| CAGAAGCAATTCATGAATTA+AGG | + | Chr7:69434455-69434474 | None:intergenic | 30.0% | |
| CTATGGTATGATAGATAAAG+TGG | + | Chr7:69434347-69434366 | None:intergenic | 30.0% | |
| GAAGATCCAAAACATATAGA+AGG | + | Chr7:69435317-69435336 | None:intergenic | 30.0% | |
| TATATGTTCATTGCTTAGGT+TGG | - | Chr7:69434562-69434581 | MsG0780039821.01.T01:CDS | 30.0% | |
| TATGTGTGTTAATGAGATGA+TGG | - | Chr7:69435386-69435405 | MsG0780039821.01.T01:exon | 30.0% | |
| TGTTCATCACATTATTCACT+TGG | - | Chr7:69434661-69434680 | MsG0780039821.01.T01:intron | 30.0% | |
| AGATTGTAAGGCAAATGCTT+AGG | + | Chr7:69435131-69435150 | None:intergenic | 35.0% | |
| AGCATTTGCCTTACAATCTA+TGG | - | Chr7:69435132-69435151 | MsG0780039821.01.T01:intron | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 69434044 | 69435473 | 69434044 | ID=MsG0780039821.01;Name=MsG0780039821.01 |
| Chr7 | mRNA | 69434044 | 69435473 | 69434044 | ID=MsG0780039821.01.T01;Parent=MsG0780039821.01;Name=MsG0780039821.01.T01;_AED=0.49;_eAED=0.49;_QI=79|1|1|1|1|1|2|120|214 |
| Chr7 | exon | 69434044 | 69434655 | 69434044 | ID=MsG0780039821.01.T01:exon:12532;Parent=MsG0780039821.01.T01 |
| Chr7 | exon | 69435242 | 69435473 | 69435242 | ID=MsG0780039821.01.T01:exon:12531;Parent=MsG0780039821.01.T01 |
| Chr7 | five_prime_UTR | 69435395 | 69435473 | 69435395 | ID=MsG0780039821.01.T01:five_prime_utr;Parent=MsG0780039821.01.T01 |
| Chr7 | CDS | 69435242 | 69435394 | 69435242 | ID=MsG0780039821.01.T01:cds;Parent=MsG0780039821.01.T01 |
| Chr7 | CDS | 69434164 | 69434655 | 69434164 | ID=MsG0780039821.01.T01:cds;Parent=MsG0780039821.01.T01 |
| Chr7 | three_prime_UTR | 69434044 | 69434163 | 69434044 | ID=MsG0780039821.01.T01:three_prime_utr;Parent=MsG0780039821.01.T01 |
| Gene Sequence |
>MsG0780039821.01.T01 ATGGCAGCTGCTTCTCCTATGGCAAGCCAACTCAAGTCCAGCTTCACTAGACCTTTGGTCACACCTAGGGGACTTTGTGGCTCTTCCCCACTCCATCAATTGCCTTCTAGAAGGCAATTTAACTTCACTGTTAAGGCAATCCAATCTGAAAAGCAAAATTACCAAGTGATTCAACCAATCAACGGTGACCCATTCATTGGAAGCCTTGAAACTCCAGTTACATCAAGCCCACTTGTTGCATGGTACCTCTCCAACCTACCAGGCTACAGAACTGCAGTGAACCCACTTCTTAGGGGAATTGAAGTCGGCTTAGCCCACGGATTCTTCCTCGTAGGCCCGTTCGTTAAAGCCGGGCCTCTAAGGAACACCGAATATGCCGGATCAGCAGGCTCTCTTGCAGCTGCTGGGCTTATTGTTATCCTAAGCATTTGCCTTACAATCTATGGAGTTTCATCATTCAATGAAGGTGATGCATCAACTGCTCCATCTTTGACCTTAACGGGCCGTAAGAAGCAGCCCGATCCGCTTCAAACAGCTGATGGTTGGTCCAAATTCACTGGTGGGTTTTTCTTTGGAGGAATTTCTGGTGTTACTTGGGCTTACTTCCTTCTATATGTTTTGGATCTTCCATACTTTGTTAAGTAA |
| Protein sequence |
>MsG0780039821.01.T01 MAAASPMASQLKSSFTRPLVTPRGLCGSSPLHQLPSRRQFNFTVKAIQSEKQNYQVIQPINGDPFIGSLETPVTSSPLVAWYLSNLPGYRTAVNPLLRGIEVGLAHGFFLVGPFVKAGPLRNTEYAGSAGSLAAAGLIVILSICLTIYGVSSFNEGDASTAPSLTLTGRKKQPDPLQTADGWSKFTGGFFFGGISGVTWAYFLLYVLDLPYFVK* |
