AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021322.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021322.01.T01 MTR_4g071550 72.355 293 42 4 1 287 1 260 9.97e-141 397
MsG0480021322.01.T01 MTR_5g033930 67.254 284 65 4 3 286 2 257 6.00e-129 367
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021322.01.T01 AT5G05670 68.844 199 52 2 88 286 71 259 3.77e-98 289
MsG0480021322.01.T01 AT5G05670 68.844 199 52 2 88 286 71 259 3.77e-98 289
MsG0480021322.01.T01 AT2G18770 59.735 226 74 5 68 286 44 259 7.35e-82 248
MsG0480021322.01.T01 AT2G18770 59.735 226 74 5 68 286 54 269 8.91e-82 248

Find 62 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTATATGTACCAGTTCT+TGG 0.230635 4:-56680998 MsG0480021322.01.T01:three_prime_UTR
GCAGATGCTTCTGGTTTAAC+TGG 0.270185 4:-56681161 MsG0480021322.01.T01:CDS
TTCTTCAGTTTCTACTCTTT+AGG 0.284102 4:-56684376 MsG0480021322.01.T01:intron
AGTCTTTGTGGTTGATGCTT+TGG 0.317524 4:-56682043 MsG0480021322.01.T01:CDS
CTGTCTGCGGATAAATTCTT+TGG 0.318615 4:+56681670 None:intergenic
GTTACAGCTGCAGATGCTTC+TGG 0.319155 4:-56681170 MsG0480021322.01.T01:CDS
TTCTCGTCTTCGACCCAAAC+TGG 0.345023 4:-56682094 MsG0480021322.01.T01:CDS
GTTACAAATGAGTTCACTTT+GGG 0.358609 4:-56681236 MsG0480021322.01.T01:CDS
AGGCAAGAACTCATCCAGTT+TGG 0.361067 4:+56682080 None:intergenic
AGAATAAAAGTGCCTTCATT+AGG 0.363687 4:+56682423 None:intergenic
TTCTATTCCTGCATCAGCTT+AGG 0.374348 4:-56682485 MsG0480021322.01.T01:CDS
GAGCACTGAGTAAAGGAAAA+TGG 0.377929 4:+56681200 None:intergenic
GTGAGATATAATCAGAATCT+TGG 0.378317 4:-56682578 MsG0480021322.01.T01:intron
TCTATTCCTGCATCAGCTTA+GGG 0.408440 4:-56682484 MsG0480021322.01.T01:CDS
GTTCATATTGTTGATGTGCC+TGG 0.408838 4:-56682120 MsG0480021322.01.T01:CDS
ATAGCTGCATAGAGTTGATT+GGG 0.413943 4:+56684455 None:intergenic
GGCAAGAACTCATCCAGTTT+GGG 0.419474 4:+56682081 None:intergenic
AGTGCATAATTTGAATAGAA+AGG 0.419575 4:+56682530 None:intergenic
GTTTAAACTGAGATTTCTGT+TGG 0.428944 4:-56681078 MsG0480021322.01.T01:three_prime_UTR
AATAGCTGCATAGAGTTGAT+TGG 0.428948 4:+56684454 None:intergenic
CCTATATGATCTATTGACTA+AGG 0.433187 4:-56681762 MsG0480021322.01.T01:CDS
AGCAGCTGGCATAGTCTTTG+TGG 0.437351 4:-56682055 MsG0480021322.01.T01:CDS
CAGATGCTTCTGGTTTAACT+GGG 0.455158 4:-56681160 MsG0480021322.01.T01:CDS
TGAGATATAATCAGAATCTT+GGG 0.473089 4:-56682577 MsG0480021322.01.T01:intron
GATAAAGTAAATCCAAGAAC+TGG 0.478119 4:+56680986 None:intergenic
TTCATATTGTTGATGTGCCT+GGG 0.478238 4:-56682119 MsG0480021322.01.T01:CDS
GAGTTCTTGCCTCAAGCAGC+TGG 0.478903 4:-56682069 MsG0480021322.01.T01:CDS
GGCACATCAACAATATGAAC+AGG 0.480812 4:+56682123 None:intergenic
TGTTACAAATGAGTTCACTT+TGG 0.486602 4:-56681237 MsG0480021322.01.T01:CDS
GAGTTCACTTTGGGAGTACC+TGG 0.488290 4:-56681227 MsG0480021322.01.T01:CDS
CCTTAGTCAATAGATCATAT+AGG 0.492682 4:+56681762 None:intergenic
GAGAATATGTTAAGCAATAG+CGG 0.497430 4:-56681109 MsG0480021322.01.T01:exon
CCAGGGTACTGTTACATCAA+TGG 0.499911 4:-56682448 MsG0480021322.01.T01:CDS
TTTGTTAGAGCACTGAGTAA+AGG 0.505548 4:+56681193 None:intergenic
GTAAAGGAAAATGGCTCGCC+AGG 0.507254 4:+56681209 None:intergenic
ATCTAGCAACTGAATGGAGA+AGG 0.513521 4:+56681983 None:intergenic
CTCACGAAAGAGAAGAAGTA+GGG 0.519654 4:+56684412 None:intergenic
ACTCACGAAAGAGAAGAAGT+AGG 0.523499 4:+56684411 None:intergenic
TAGGGATGGTTCTACTCACC+AGG 0.530858 4:-56682466 MsG0480021322.01.T01:CDS
AACCATCCCTAAGCTGATGC+AGG 0.534380 4:+56682478 None:intergenic
TTCCTGCATCAGCTTAGGGA+TGG 0.541638 4:-56682480 MsG0480021322.01.T01:CDS
AGAATATGTTAAGCAATAGC+GGG 0.544006 4:-56681108 MsG0480021322.01.T01:exon
CTATATGATCTATTGACTAA+GGG 0.579425 4:-56681761 MsG0480021322.01.T01:CDS
ACATCAATGGAACCTAATGA+AGG 0.581103 4:-56682435 MsG0480021322.01.T01:CDS
TCTTCACGGTGAAACTGCAA+AGG 0.591155 4:-56682403 MsG0480021322.01.T01:intron
AAGACTATGCCAGCTGCTTG+AGG 0.591483 4:+56682060 None:intergenic
TGCAACAAGAATTAGAGCAA+TGG 0.594629 4:-56684534 MsG0480021322.01.T01:CDS
TTTCCTTCTCGATCTGTCTG+CGG 0.600971 4:+56681657 None:intergenic
TCTAGCAACTGAATGGAGAA+GGG 0.608882 4:+56681984 None:intergenic
GGTCGAAGACGAGAATGCCC+AGG 0.623720 4:+56682102 None:intergenic
GGAATTACTCTGAGGCAGCA+CGG 0.624997 4:+56682005 None:intergenic
ACAAGAATTAGAGCAATGGA+AGG 0.635265 4:-56684530 MsG0480021322.01.T01:CDS
TAGCTGCATAGAGTTGATTG+GGG 0.653502 4:+56684456 None:intergenic
TTGTTGCACAGAATAAGCAG+AGG 0.654972 4:+56681716 None:intergenic
TGACTAAGGGAAGTGTTGTG+AGG 0.663752 4:-56681748 MsG0480021322.01.T01:CDS
TATCCGCAGACAGATCGAGA+AGG 0.678549 4:-56681660 MsG0480021322.01.T01:CDS
ATTACGGTCATCAAGAAGTG+CGG 0.678648 4:-56681273 MsG0480021322.01.T01:CDS
CCATTGATGTAACAGTACCC+TGG 0.685400 4:+56682448 None:intergenic
AGATGCTTCTGGTTTAACTG+GGG 0.698873 4:-56681159 MsG0480021322.01.T01:CDS
AGCTGCATAGAGTTGATTGG+GGG 0.724048 4:+56684457 None:intergenic
AGGGATGGTTCTACTCACCA+GGG 0.731933 4:-56682465 MsG0480021322.01.T01:CDS
TGGAGAAGGGAATTACTCTG+AGG 0.732266 4:+56681997 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAGAATTAATTGATA+AGG + Chr4:56683944-56683963 None:intergenic 15.0%
!! TAGTTTAAAGAATAAATTGA+TGG - Chr4:56682384-56682403 MsG0480021322.01.T01:intron 15.0%
!!! ACATTTTGTTTTATGATTTA+AGG - Chr4:56683729-56683748 MsG0480021322.01.T01:intron 15.0%
!!! CATTATAATAAGTTGATAAA+TGG - Chr4:56681568-56681587 MsG0480021322.01.T01:intron 15.0%
!!! TATTATAATGTTGACTATAT+CGG + Chr4:56681558-56681577 None:intergenic 15.0%
!!! TTAATAATAAGTTGGATAAA+TGG - Chr4:56681408-56681427 MsG0480021322.01.T01:intron 15.0%
!!! TTAATTTGTGAAATAAAGAT+TGG - Chr4:56682411-56682430 MsG0480021322.01.T01:CDS 15.0%
!!! TTATTAATGTTGACTTATAT+CGG + Chr4:56681395-56681414 None:intergenic 15.0%
!! AGTCAACATTAATAATAAGT+TGG - Chr4:56681400-56681419 MsG0480021322.01.T01:intron 20.0%
!! ATAAGTTTGAACATTTGTTT+GGG - Chr4:56683175-56683194 MsG0480021322.01.T01:intron 20.0%
!! TATAAGTTTGAACATTTGTT+TGG - Chr4:56683174-56683193 MsG0480021322.01.T01:intron 20.0%
!!! AAGATTTAAGATTAATGAGT+TGG - Chr4:56681804-56681823 MsG0480021322.01.T01:intron 20.0%
!!! ATGTTAAACCTAAATTCTTT+TGG - Chr4:56681312-56681331 MsG0480021322.01.T01:intron 20.0%
!!! TGAAATGAATTGATTTCTTT+AGG - Chr4:56682713-56682732 MsG0480021322.01.T01:intron 20.0%
!!! TTTTAGAAACTAACCTAAAA+AGG + Chr4:56682239-56682258 None:intergenic 20.0%
! AAGTATGTTATTACATTAGC+AGG - Chr4:56682906-56682925 MsG0480021322.01.T01:intron 25.0%
! ACACTATAAGAGAAACTATT+AGG - Chr4:56681780-56681799 MsG0480021322.01.T01:intron 25.0%
! ACTCAATAAAACGATTCATT+TGG - Chr4:56684134-56684153 MsG0480021322.01.T01:intron 25.0%
! AGTGCATAATTTGAATAGAA+AGG + Chr4:56682989-56683008 None:intergenic 25.0%
! AGTGTGAAGATAAATAACTT+TGG + Chr4:56684117-56684136 None:intergenic 25.0%
! ATTTCTTTAGGTCTAATCTA+AGG - Chr4:56682725-56682744 MsG0480021322.01.T01:intron 25.0%
! CAACTAAACCAAAAGAATTT+AGG + Chr4:56681323-56681342 None:intergenic 25.0%
! CTGAATAATCAAATGAGTAA+AGG + Chr4:56683915-56683934 None:intergenic 25.0%
! CTGATTTCACAACTATTAAT+AGG + Chr4:56683308-56683327 None:intergenic 25.0%
! GATATTGCCTATTAAACAAT+TGG - Chr4:56681355-56681374 MsG0480021322.01.T01:intron 25.0%
! GTCTCTATCTATATAGTATA+TGG - Chr4:56682340-56682359 MsG0480021322.01.T01:intron 25.0%
! GTTCGATTATAAACCATATT+TGG + Chr4:56681832-56681851 None:intergenic 25.0%
! TAAATGATGCATCGTATTAA+AGG + Chr4:56681504-56681523 None:intergenic 25.0%
! TGAGATATAATCAGAATCTT+GGG - Chr4:56682939-56682958 MsG0480021322.01.T01:intron 25.0%
! TGGTTTATAATCGAACATTA+TGG - Chr4:56681836-56681855 MsG0480021322.01.T01:intron 25.0%
! TTAATATTTACTGTACAGCA+TGG - Chr4:56683591-56683610 MsG0480021322.01.T01:intron 25.0%
! TTGGATGTAATTCAATGTTT+CGG - Chr4:56681437-56681456 MsG0480021322.01.T01:intron 25.0%
!! CTATATGATCTATTGACTAA+GGG - Chr4:56683755-56683774 MsG0480021322.01.T01:intron 25.0%
!! GATGCATCATTTAGTTTAAT+GGG - Chr4:56681511-56681530 MsG0480021322.01.T01:intron 25.0%
!! TCTCTATTTTACGAAAAACT+AGG + Chr4:56682792-56682811 None:intergenic 25.0%
!! TCTTTTCTGTTATAGTTCTT+TGG - Chr4:56682487-56682506 MsG0480021322.01.T01:CDS 25.0%
!! TTAATGGGTAAAGATTGTAT+CGG - Chr4:56681685-56681704 MsG0480021322.01.T01:CDS 25.0%
!! TTATAGTTCTTTGGCAATTT+TGG - Chr4:56682496-56682515 MsG0480021322.01.T01:CDS 25.0%
!!! TTGTGAGAATTCTTTGTTTT+AGG - Chr4:56682677-56682696 MsG0480021322.01.T01:intron 25.0%
!!! TTTGTTTTGATTTTGTAGTG+CGG - Chr4:56681190-56681209 MsG0480021322.01.T01:CDS 25.0%
AAAGGATCCGAATATTATGA+TGG - Chr4:56682636-56682655 MsG0480021322.01.T01:intron 30.0%
AAGAAACGTAAGCTAATACT+TGG + Chr4:56683346-56683365 None:intergenic 30.0%
AATAAAGATTGGATGCATGA+TGG - Chr4:56682422-56682441 MsG0480021322.01.T01:CDS 30.0%
AATGTAATCTAGCAACTGAA+TGG + Chr4:56683542-56683561 None:intergenic 30.0%
AATTACCTGATAGAAGAGAA+CGG + Chr4:56681277-56681296 None:intergenic 30.0%
ACAACTATACAAGCGATATT+TGG + Chr4:56682838-56682857 None:intergenic 30.0%
AGAATATGTTAAGCAATAGC+GGG - Chr4:56684408-56684427 MsG0480021322.01.T01:CDS 30.0%
AGTAAAGGTAGACAAAGTTA+AGG + Chr4:56683900-56683919 None:intergenic 30.0%
ATAAAGATTGGATGCATGAT+GGG - Chr4:56682423-56682442 MsG0480021322.01.T01:CDS 30.0%
ATAATAGTCTCCAAGAAAGA+AGG + Chr4:56682579-56682598 None:intergenic 30.0%
ATATAACAACCCTTCTTTCT+TGG - Chr4:56682566-56682585 MsG0480021322.01.T01:CDS 30.0%
ATATGAGTCAATAGTAGATC+TGG + Chr4:56684204-56684223 None:intergenic 30.0%
ATGGTAAAGATTTGTATCAC+CGG - Chr4:56681529-56681548 MsG0480021322.01.T01:intron 30.0%
CCTTAGTCAATAGATCATAT+AGG + Chr4:56683757-56683776 None:intergenic 30.0%
GAAGAAAGAGTGATAGTTTA+GGG + Chr4:56681994-56682013 None:intergenic 30.0%
GAGAATATGTTAAGCAATAG+CGG - Chr4:56684407-56684426 MsG0480021322.01.T01:CDS 30.0%
GATAAAGTAAATCCAAGAAC+TGG + Chr4:56684533-56684552 None:intergenic 30.0%
GCAATTCAGTTATTGTTACA+AGG - Chr4:56682751-56682770 MsG0480021322.01.T01:intron 30.0%
GTGAGATATAATCAGAATCT+TGG - Chr4:56682938-56682957 MsG0480021322.01.T01:intron 30.0%
GTTACAAATGAGTTCACTTT+GGG - Chr4:56684280-56684299 MsG0480021322.01.T01:intron 30.0%
GTTAGTATTGTAAGTAGAGT+TGG - Chr4:56681709-56681728 MsG0480021322.01.T01:CDS 30.0%
GTTTAAACTGAGATTTCTGT+TGG - Chr4:56684438-56684457 MsG0480021322.01.T01:CDS 30.0%
TAATAGTCTCCAAGAAAGAA+GGG + Chr4:56682578-56682597 None:intergenic 30.0%
TCAATGTTGTTACTGTTGAT+TGG - Chr4:56684038-56684057 MsG0480021322.01.T01:intron 30.0%
TCATCATAGAGTAATTTGAG+TGG + Chr4:56682214-56682233 None:intergenic 30.0%
TGAAGAAAGAGTGATAGTTT+AGG + Chr4:56681995-56682014 None:intergenic 30.0%
TGATAGTTTAGGGACTAAAA+TGG + Chr4:56681984-56682003 None:intergenic 30.0%
TGTCTCTCCAATTGTTTAAT+AGG + Chr4:56681365-56681384 None:intergenic 30.0%
TGTTACAAATGAGTTCACTT+TGG - Chr4:56684279-56684298 MsG0480021322.01.T01:intron 30.0%
TTCTTCAGTTTCTACTCTTT+AGG - Chr4:56681140-56681159 MsG0480021322.01.T01:CDS 30.0%
! AGGAACAATTTTCTCATCTA+TGG - Chr4:56681006-56681025 MsG0480021322.01.T01:three_prime_UTR 30.0%
! ATTTTCTCATCTATGGAATG+TGG - Chr4:56681013-56681032 MsG0480021322.01.T01:three_prime_UTR 30.0%
! CCTATATGATCTATTGACTA+AGG - Chr4:56683754-56683773 MsG0480021322.01.T01:intron 30.0%
! CGATGCATCATTTAGTTTAA+TGG - Chr4:56681510-56681529 MsG0480021322.01.T01:intron 30.0%
! CTAAAAAAGTTTTACACCGT+CGG - Chr4:56682304-56682323 MsG0480021322.01.T01:intron 30.0%
! GTATATGGACTAAATTGAAG+AGG - Chr4:56682355-56682374 MsG0480021322.01.T01:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG - Chr4:56681816-56681835 MsG0480021322.01.T01:intron 30.0%
! TCTACCTGACATGATTTTAT+TGG - Chr4:56682273-56682292 MsG0480021322.01.T01:intron 30.0%
!! AGAATAAAAGTGCCTTCATT+AGG + Chr4:56683096-56683115 None:intergenic 30.0%
!! AGTTGATAAATGGAAGTGAT+TGG - Chr4:56681578-56681597 MsG0480021322.01.T01:intron 30.0%
!!! TTGGTTTTATGTCAAGAAAG+TGG - Chr4:56684153-56684172 MsG0480021322.01.T01:intron 30.0%
!!! TTGTTTTGATTTTGTAGTGC+GGG - Chr4:56681191-56681210 MsG0480021322.01.T01:CDS 30.0%
AACCCCACCATCATAATATT+CGG + Chr4:56682646-56682665 None:intergenic 35.0%
AATAGCAAGGACCAAATTGA+CGG + Chr4:56682047-56682066 None:intergenic 35.0%
ACAAGAATTAGAGCAATGGA+AGG - Chr4:56680986-56681005 MsG0480021322.01.T01:three_prime_UTR 35.0%
ACATCAATGGAACCTAATGA+AGG - Chr4:56683081-56683100 MsG0480021322.01.T01:intron 35.0%
ACTTAATCCAGTCAACACAA+TGG + Chr4:56681241-56681260 None:intergenic 35.0%
CAACTATATGTACCAGTTCT+TGG - Chr4:56684518-56684537 MsG0480021322.01.T01:CDS 35.0%
CAATGAATGAAACGCTATGT+AGG - Chr4:56682460-56682479 MsG0480021322.01.T01:CDS 35.0%
GAACAAACCCTTCAAACAAA+AGG + Chr4:56682615-56682634 None:intergenic 35.0%
GATCCGAATATTATGATGGT+GGG - Chr4:56682640-56682659 MsG0480021322.01.T01:intron 35.0%
GCATCCAATAAAATCATGTC+AGG + Chr4:56682280-56682299 None:intergenic 35.0%
GCTTGTATAGTTGTATCTGA+GGG - Chr4:56682844-56682863 MsG0480021322.01.T01:intron 35.0%
GTTGGATAAATGGAAGTGAT+TGG - Chr4:56681418-56681437 MsG0480021322.01.T01:intron 35.0%
TCTCTAACATCTCAATAGCA+AGG + Chr4:56682060-56682079 None:intergenic 35.0%
TCTGGCAGATGAAAATAAAC+TGG + Chr4:56684186-56684205 None:intergenic 35.0%
TGCAACAAGAATTAGAGCAA+TGG - Chr4:56680982-56681001 MsG0480021322.01.T01:three_prime_UTR 35.0%
TTAAACAATTGGAGAGACAC+CGG - Chr4:56681366-56681385 MsG0480021322.01.T01:intron 35.0%
TTAAGCTGGGACTAAGATTT+GGG - Chr4:56682521-56682540 MsG0480021322.01.T01:CDS 35.0%
TTACATTAGCAGGTGATACA+TGG - Chr4:56682916-56682935 MsG0480021322.01.T01:intron 35.0%
TTCATATTGTTGATGTGCCT+GGG - Chr4:56683397-56683416 MsG0480021322.01.T01:intron 35.0%
! AATAGCTGCATAGAGTTGAT+TGG + Chr4:56681065-56681084 None:intergenic 35.0%
! ATAGCTGCATAGAGTTGATT+GGG + Chr4:56681064-56681083 None:intergenic 35.0%
! ATCCGAATATTATGATGGTG+GGG - Chr4:56682641-56682660 MsG0480021322.01.T01:intron 35.0%
! ATGTTGACTATATCGGTATC+CGG + Chr4:56681551-56681570 None:intergenic 35.0%
! CAACACAATGGTGTTAGATT+TGG + Chr4:56681229-56681248 None:intergenic 35.0%
! CTCTATGATGACACCTTTTT+AGG - Chr4:56682223-56682242 MsG0480021322.01.T01:intron 35.0%
! GTTTGAAGGGTTTGTTCAAA+AGG - Chr4:56682618-56682637 MsG0480021322.01.T01:intron 35.0%
! TCATGTCAGGTAGATAGTTT+TGG + Chr4:56682267-56682286 None:intergenic 35.0%
!! ATTGTGTTGACTGGATTAAG+TGG - Chr4:56681240-56681259 MsG0480021322.01.T01:CDS 35.0%
!! GCATCATCCTTTTGTTTGAA+GGG - Chr4:56682605-56682624 MsG0480021322.01.T01:intron 35.0%
!! TGCATCATCCTTTTGTTTGA+AGG - Chr4:56682604-56682623 MsG0480021322.01.T01:intron 35.0%
!! TGTTGACTTATATCGGTATC+CGG + Chr4:56681388-56681407 None:intergenic 35.0%
!! TTGTGTTGACTGGATTAAGT+GGG - Chr4:56681241-56681260 MsG0480021322.01.T01:CDS 35.0%
!! TTTGTTAGAGCACTGAGTAA+AGG + Chr4:56684326-56684345 None:intergenic 35.0%
!!! CCTTTTTCAGCGACAAATTA+CGG - Chr4:56684227-56684246 MsG0480021322.01.T01:intron 35.0%
!!! GAAGGCACTTTTATTCTTCA+CGG - Chr4:56683099-56683118 MsG0480021322.01.T01:intron 35.0%
!!! TTACAAGGTTTTGAACTGCA+TGG - Chr4:56682766-56682785 MsG0480021322.01.T01:intron 35.0%
ACTAATATCCCTTCTTCTGC+AGG + Chr4:56683693-56683712 None:intergenic 40.0%
ACTCACGAAAGAGAAGAAGT+AGG + Chr4:56681108-56681127 None:intergenic 40.0%
AGGATAGTCAGATTGCAAAG+AGG - Chr4:56681740-56681759 MsG0480021322.01.T01:CDS 40.0%
ATAGCAAGGACCAAATTGAC+GGG + Chr4:56682046-56682065 None:intergenic 40.0%
ATCCAGTGTCCAAAATGTGT+CGG + Chr4:56681632-56681651 None:intergenic 40.0%
ATCTAGCAACTGAATGGAGA+AGG + Chr4:56683536-56683555 None:intergenic 40.0%
ATTACGGTCATCAAGAAGTG+CGG - Chr4:56684243-56684262 MsG0480021322.01.T01:intron 40.0%
CAAAACCGTTCTCTTCTATC+AGG - Chr4:56681269-56681288 MsG0480021322.01.T01:CDS 40.0%
CACAAACACACATTCAGAAG+GGG + Chr4:56683997-56684016 None:intergenic 40.0%
CACATTTGGACACTGGATAA+TGG - Chr4:56681473-56681492 MsG0480021322.01.T01:intron 40.0%
CAGATCGAGAAGGAAATGTA+TGG - Chr4:56683866-56683885 MsG0480021322.01.T01:intron 40.0%
CCACAAACACACATTCAGAA+GGG + Chr4:56683998-56684017 None:intergenic 40.0%
CCTTCTGAATGTGTGTTTGT+GGG - Chr4:56683996-56684015 MsG0480021322.01.T01:intron 40.0%
CGCTTGTATAGTTGTATCTG+AGG - Chr4:56682843-56682862 MsG0480021322.01.T01:intron 40.0%
CTCACGAAAGAGAAGAAGTA+GGG + Chr4:56681107-56681126 None:intergenic 40.0%
CTGTCTGCGGATAAATTCTT+TGG + Chr4:56683849-56683868 None:intergenic 40.0%
CTTAAGCTGGGACTAAGATT+TGG - Chr4:56682520-56682539 MsG0480021322.01.T01:CDS 40.0%
GACAAATTTCTATGCACCGA+CGG + Chr4:56682323-56682342 None:intergenic 40.0%
GGATCCGAATATTATGATGG+TGG - Chr4:56682639-56682658 MsG0480021322.01.T01:intron 40.0%
GGCACATCAACAATATGAAC+AGG + Chr4:56683396-56683415 None:intergenic 40.0%
GTTCATATTGTTGATGTGCC+TGG - Chr4:56683396-56683415 MsG0480021322.01.T01:intron 40.0%
TAAGCTGGGACTAAGATTTG+GGG - Chr4:56682522-56682541 MsG0480021322.01.T01:CDS 40.0%
TATCCAGTGTCCAAATGTGT+CGG + Chr4:56681472-56681491 None:intergenic 40.0%
TCTAGCAACTGAATGGAGAA+GGG + Chr4:56683535-56683554 None:intergenic 40.0%
TCTATTCCTGCATCAGCTTA+GGG - Chr4:56683032-56683051 MsG0480021322.01.T01:intron 40.0%
TTCTATTCCTGCATCAGCTT+AGG - Chr4:56683031-56683050 MsG0480021322.01.T01:intron 40.0%
TTCTGCAGGAACATGAGAAA+TGG + Chr4:56683679-56683698 None:intergenic 40.0%
TTGTTGCACAGAATAAGCAG+AGG + Chr4:56683803-56683822 None:intergenic 40.0%
! ACACATTTTGGACACTGGAT+TGG - Chr4:56681632-56681651 MsG0480021322.01.T01:intron 40.0%
! AGTCTTTGTGGTTGATGCTT+TGG - Chr4:56683473-56683492 MsG0480021322.01.T01:intron 40.0%
! CCGTAATTTGTCGCTGAAAA+AGG + Chr4:56684230-56684249 None:intergenic 40.0%
! TAAGGCTTGGTTGTTGTTGT+TGG - Chr4:56681897-56681916 MsG0480021322.01.T01:intron 40.0%
! TAGCTGCATAGAGTTGATTG+GGG + Chr4:56681063-56681082 None:intergenic 40.0%
! TCTAACACCATTGTGTTGAC+TGG - Chr4:56681231-56681250 MsG0480021322.01.T01:CDS 40.0%
! TGTAAGTAGAGTTGGTCAGA+TGG - Chr4:56681717-56681736 MsG0480021322.01.T01:CDS 40.0%
! TGTGTGATACCGACACATTT+TGG - Chr4:56681460-56681479 MsG0480021322.01.T01:intron 40.0%
!! AGATGCTTCTGGTTTAACTG+GGG - Chr4:56684357-56684376 MsG0480021322.01.T01:intron 40.0%
!! CAGATGCTTCTGGTTTAACT+GGG - Chr4:56684356-56684375 MsG0480021322.01.T01:intron 40.0%
!! GAGCACTGAGTAAAGGAAAA+TGG + Chr4:56684319-56684338 None:intergenic 40.0%
!!! GGCAATTTTGGTCTTAAGCT+GGG - Chr4:56682508-56682527 MsG0480021322.01.T01:CDS 40.0%
!!! TGGCAATTTTGGTCTTAAGC+TGG - Chr4:56682507-56682526 MsG0480021322.01.T01:CDS 40.0%
AAACACACATTCAGAAGGGG+TGG + Chr4:56683994-56684013 None:intergenic 45.0%
AAAGAAGAGTCAGGGTTAGG+TGG - Chr4:56682868-56682887 MsG0480021322.01.T01:intron 45.0%
AACACACATTCAGAAGGGGT+GGG + Chr4:56683993-56684012 None:intergenic 45.0%
AGGCAAGAACTCATCCAGTT+TGG + Chr4:56683439-56683458 None:intergenic 45.0%
ATAAGATCCGCCCGTCAATT+TGG - Chr4:56682033-56682052 MsG0480021322.01.T01:CDS 45.0%
ATACCGACACATTTGGACAC+TGG - Chr4:56681466-56681485 MsG0480021322.01.T01:intron 45.0%
CCATTGATGTAACAGTACCC+TGG + Chr4:56683071-56683090 None:intergenic 45.0%
CCCACAAACACACATTCAGA+AGG + Chr4:56683999-56684018 None:intergenic 45.0%
CCCTTCTGAATGTGTGTTTG+TGG - Chr4:56683995-56684014 MsG0480021322.01.T01:intron 45.0%
CTCATGTTCCTGCAGAAGAA+GGG - Chr4:56683682-56683701 MsG0480021322.01.T01:intron 45.0%
CTGAGGGAAAAGAAGAGTCA+GGG - Chr4:56682860-56682879 MsG0480021322.01.T01:intron 45.0%
GGAAAAGAAGAGTCAGGGTT+AGG - Chr4:56682865-56682884 MsG0480021322.01.T01:intron 45.0%
GGCAAGAACTCATCCAGTTT+GGG + Chr4:56683438-56683457 None:intergenic 45.0%
GTCAGATTGCAAAGAGGTAG+AGG - Chr4:56681746-56681765 MsG0480021322.01.T01:CDS 45.0%
GTGTGTGATACCGACACATT+TGG - Chr4:56681459-56681478 MsG0480021322.01.T01:intron 45.0%
TAGGCAATATCCCGCGAATT+CGG + Chr4:56681346-56681365 None:intergenic 45.0%
TCTCATGTTCCTGCAGAAGA+AGG - Chr4:56683681-56683700 MsG0480021322.01.T01:intron 45.0%
TCTGAGGGAAAAGAAGAGTC+AGG - Chr4:56682859-56682878 MsG0480021322.01.T01:intron 45.0%
TCTTCACGGTGAAACTGCAA+AGG - Chr4:56683113-56683132 MsG0480021322.01.T01:intron 45.0%
TGGAGAAGGGAATTACTCTG+AGG + Chr4:56683522-56683541 None:intergenic 45.0%
TGGTTTAGTTGCCGAATTCG+CGG - Chr4:56681332-56681351 MsG0480021322.01.T01:intron 45.0%
TTGACTGGATTAAGTGGGAG+TGG - Chr4:56681246-56681265 MsG0480021322.01.T01:CDS 45.0%
TTTCCTTCTCGATCTGTCTG+CGG + Chr4:56683862-56683881 None:intergenic 45.0%
! AAGCCTTATCCCACTAAGCA+GGG + Chr4:56681885-56681904 None:intergenic 45.0%
! AAGTAGAGTTGGTCAGATGG+AGG - Chr4:56681720-56681739 MsG0480021322.01.T01:CDS 45.0%
! AGCTGCATAGAGTTGATTGG+GGG + Chr4:56681062-56681081 None:intergenic 45.0%
! TACCGACACATTTTGGACAC+TGG - Chr4:56681627-56681646 MsG0480021322.01.T01:intron 45.0%
! TGGGACTAAGATTTGGGGTT+TGG - Chr4:56682527-56682546 MsG0480021322.01.T01:CDS 45.0%
!! CCAGGGTACTGTTACATCAA+TGG - Chr4:56683068-56683087 MsG0480021322.01.T01:intron 45.0%
!! GCAGATGCTTCTGGTTTAAC+TGG - Chr4:56684355-56684374 MsG0480021322.01.T01:intron 45.0%
!! TGACTAAGGGAAGTGTTGTG+AGG - Chr4:56683768-56683787 MsG0480021322.01.T01:intron 45.0%
AACCATCCCTAAGCTGATGC+AGG + Chr4:56683041-56683060 None:intergenic 50.0%
AAGACTATGCCAGCTGCTTG+AGG + Chr4:56683459-56683478 None:intergenic 50.0%
ACACACATTCAGAAGGGGTG+GGG + Chr4:56683992-56684011 None:intergenic 50.0%
AGCAGCTGGCATAGTCTTTG+TGG - Chr4:56683461-56683480 MsG0480021322.01.T01:intron 50.0%
CACTCAAGCCAGTGTTTGAG+TGG + Chr4:56683154-56683173 None:intergenic 50.0%
CTGCTTAGTGGGATAAGGCT+TGG - Chr4:56681884-56681903 MsG0480021322.01.T01:intron 50.0%
CTTATCCCACTAAGCAGGGT+CGG + Chr4:56681881-56681900 None:intergenic 50.0%
GGTTTAGTTGCCGAATTCGC+GGG - Chr4:56681333-56681352 MsG0480021322.01.T01:intron 50.0%
GTAAAGGAAAATGGCTCGCC+AGG + Chr4:56684310-56684329 None:intergenic 50.0%
GTTACAGCTGCAGATGCTTC+TGG - Chr4:56684346-56684365 MsG0480021322.01.T01:intron 50.0%
TATCCGCAGACAGATCGAGA+AGG - Chr4:56683856-56683875 MsG0480021322.01.T01:intron 50.0%
TCAAGCCAGTGTTTGAGTGG+AGG + Chr4:56683151-56683170 None:intergenic 50.0%
TTCCTGCATCAGCTTAGGGA+TGG - Chr4:56683036-56683055 MsG0480021322.01.T01:intron 50.0%
TTCTCGTCTTCGACCCAAAC+TGG - Chr4:56683422-56683441 MsG0480021322.01.T01:intron 50.0%
! AGGGATGGTTCTACTCACCA+GGG - Chr4:56683051-56683070 MsG0480021322.01.T01:intron 50.0%
! CAAGCCTTATCCCACTAAGC+AGG + Chr4:56681886-56681905 None:intergenic 50.0%
! TAGGGATGGTTCTACTCACC+AGG - Chr4:56683050-56683069 MsG0480021322.01.T01:intron 50.0%
!! ATGATGGTGGGGTTGTGTAG+TGG - Chr4:56682652-56682671 MsG0480021322.01.T01:intron 50.0%
!! GAGTTCACTTTGGGAGTACC+TGG - Chr4:56684289-56684308 MsG0480021322.01.T01:intron 50.0%
!! GGAATTACTCTGAGGCAGCA+CGG + Chr4:56683514-56683533 None:intergenic 50.0%
AAGTAGCCGACCCTGCTTAG+TGG - Chr4:56681872-56681891 MsG0480021322.01.T01:intron 55.0%
ACTAAGCAGGGTCGGCTACT+TGG + Chr4:56681873-56681892 None:intergenic 55.0%
AGCCAGTGTTTGAGTGGAGG+AGG + Chr4:56683148-56683167 None:intergenic 55.0%
AGCCTCCTCCACTCAAACAC+TGG - Chr4:56683143-56683162 MsG0480021322.01.T01:intron 55.0%
AGTAGCCGACCCTGCTTAGT+GGG - Chr4:56681873-56681892 MsG0480021322.01.T01:intron 55.0%
CGACCCTGCTTAGTGGGATA+AGG - Chr4:56681879-56681898 MsG0480021322.01.T01:intron 55.0%
GAGTTCTTGCCTCAAGCAGC+TGG - Chr4:56683447-56683466 MsG0480021322.01.T01:intron 55.0%
GCAAGGACCAAATTGACGGG+CGG + Chr4:56682043-56682062 None:intergenic 55.0%
GGTCGAAGACGAGAATGCCC+AGG + Chr4:56683417-56683436 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56680981 56684557 56680981 ID=MsG0480021322.01;Name=MsG0480021322.01
Chr4 mRNA 56680981 56684557 56680981 ID=MsG0480021322.01.T01;Parent=MsG0480021322.01;Name=MsG0480021322.01.T01;_AED=0.41;_eAED=0.49;_QI=0|0.4|0.33|1|0.4|0.16|6|131|287
Chr4 exon 56680981 56681301 56680981 ID=MsG0480021322.01.T01:exon:7118;Parent=MsG0480021322.01.T01
Chr4 exon 56681656 56681787 56681656 ID=MsG0480021322.01.T01:exon:7117;Parent=MsG0480021322.01.T01
Chr4 exon 56681919 56681929 56681919 ID=MsG0480021322.01.T01:exon:7116;Parent=MsG0480021322.01.T01
Chr4 exon 56681993 56682160 56681993 ID=MsG0480021322.01.T01:exon:7115;Parent=MsG0480021322.01.T01
Chr4 exon 56682404 56682585 56682404 ID=MsG0480021322.01.T01:exon:7114;Parent=MsG0480021322.01.T01
Chr4 exon 56684377 56684557 56684377 ID=MsG0480021322.01.T01:exon:7113;Parent=MsG0480021322.01.T01
Chr4 CDS 56684377 56684557 56684377 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 CDS 56682404 56682585 56682404 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 CDS 56681993 56682160 56681993 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 CDS 56681919 56681929 56681919 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 CDS 56681656 56681787 56681656 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 CDS 56681112 56681301 56681112 ID=MsG0480021322.01.T01:cds;Parent=MsG0480021322.01.T01
Chr4 three_prime_UTR 56680981 56681111 56680981 ID=MsG0480021322.01.T01:three_prime_utr;Parent=MsG0480021322.01.T01
Gene Sequence

>MsG0480021322.01.T01

ATGCAACAAGAATTAGAGCAATGGAAGGAACAATTTTCTCATCTATGGAATGTGGCAAACGATTATCTTCGCGATGTTCCCCCAATCAACTCTATGCAGCTATTGCTATCGCGATTTTCACAACCCTACTTCTTCTCTTTCGTGAGTTTCTACAATTTGTTCTTCAGTTTCTACTCTTTAGAATCTTGGGTAATATTATTATTACTATTAATTTCCTTTCTATTCAAATTATGCACTAACATAATGTTCTTGACACACTTTCTATTCCTGCATCAGCTTAGGGATGGTTCTACTCACCAGGGTACTGTTACATCAATGGAACCTAATGAAGGCACTTTTATTCTTCACGGTGAAACTGCAAAGAAGAGCAAGATAAAACCTGTTCATATTGTTGATGTGCCTGGGCATTCTCGTCTTCGACCCAAACTGGATGAGTTCTTGCCTCAAGCAGCTGGCATAGTCTTTGTGGTTGATGCTTTGGACTTCTTACAAATTGCCGTGCTGCCTCAGAGTAATTCCCTTCTCCATTCACATGGTGAGTTGTACCTATATGATCTATTGACTAAGGGAAGTGTTGTGAGGAAGAAGATTCCTCTGCTTATTCTGTGCAACAAAACAGACAAAGTTACTGCTCACACCAAAGAATTTATCCGCAGACAGATCGAGAAGGAAATCGACAAATTACGGTCATCAAGAAGTGCGGTTTCAGAAGCAGATGTTACAAATGAGTTCACTTTGGGAGTACCTGGCGAGCCATTTTCCTTTACTCAGTGCTCTAACAAAGTTACAGCTGCAGATGCTTCTGGTTTAACTGGGGAGATATCTCAGCTACATGAGTTTATCAGAGAATATGTTAAGCAATAG
Protein sequence

>MsG0480021322.01.T01

MQQELEQWKEQFSHLWNVANDYLRDVPPINSMQLLLSRFSQPYFFSFVSFYNLFFSFYSLESWVILLLLLISFLFKLCTNIMFLTHFLFLHQLRDGSTHQGTVTSMEPNEGTFILHGETAKKSKIKPVHIVDVPGHSRLRPKLDEFLPQAAGIVFVVDALDFLQIAVLPQSNSLLHSHGELYLYDLLTKGSVVRKKIPLLILCNKTDKVTAHTKEFIRRQIEKEIDKLRSSRSAVSEADVTNEFTLGVPGEPFSFTQCSNKVTAADASGLTGEISQLHEFIREYVKQ*