Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024059.01.T01 | XP_039690755.1 | 94.915 | 59 | 3 | 0 | 1 | 59 | 1 | 59 | 2.44E-34 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024059.01.T01 | Q680P8 | 92.593 | 54 | 4 | 0 | 1 | 54 | 1 | 54 | 1.82E-31 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024059.01.T01 | A0A2J6KU64 | 98.214 | 56 | 1 | 0 | 1 | 56 | 1 | 56 | 1.52e-33 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048922.01 | MsG0580024059.01 | 0.809738 | 1.631537e-50 | 9.656130e-48 |
| MsG0180000096.01 | MsG0580024059.01 | 0.832046 | 1.181607e-55 | 1.305609e-52 |
| MsG0180000118.01 | MsG0580024059.01 | 0.854982 | 8.559998e-62 | 1.946515e-58 |
| MsG0180000456.01 | MsG0580024059.01 | 0.813437 | 2.561331e-51 | 1.672633e-48 |
| MsG0180003897.01 | MsG0580024059.01 | 0.834286 | 3.272969e-56 | 3.866579e-53 |
| MsG0180004609.01 | MsG0580024059.01 | 0.820155 | 7.965790e-53 | 6.253869e-50 |
| MsG0180004625.01 | MsG0580024059.01 | 0.837849 | 4.078057e-57 | 5.364991e-54 |
| MsG0180004829.01 | MsG0580024059.01 | 0.823068 | 1.690235e-53 | 1.440287e-50 |
| MsG0180005384.01 | MsG0580024059.01 | 0.854732 | 1.012001e-61 | 2.282121e-58 |
| MsG0280011308.01 | MsG0580024059.01 | 0.871096 | 8.865636e-67 | 3.531851e-63 |
| MsG0480019165.01 | MsG0580024059.01 | 0.861451 | 1.016655e-63 | 2.878257e-60 |
| MsG0480019632.01 | MsG0580024059.01 | 0.804032 | 2.623952e-49 | 1.338918e-46 |
| MsG0480020911.01 | MsG0580024059.01 | 0.821304 | 4.334428e-53 | 3.513205e-50 |
| MsG0480021279.01 | MsG0580024059.01 | 0.898489 | 5.046909e-77 | 5.978233e-73 |
| MsG0480022006.01 | MsG0580024059.01 | 0.846675 | 1.871375e-59 | 3.246151e-56 |
| MsG0480022789.01 | MsG0580024059.01 | 0.898864 | 3.490976e-77 | 4.205896e-73 |
| MsG0480023380.01 | MsG0580024059.01 | 0.806454 | 8.161325e-50 | 4.431707e-47 |
| MsG0480023774.01 | MsG0580024059.01 | 0.840764 | 7.142315e-58 | 1.027722e-54 |
| MsG0480023941.01 | MsG0580024059.01 | 0.906055 | 2.232848e-80 | 3.717371e-76 |
| MsG0580024059.01 | MsG0580024481.01 | 0.853957 | 1.694412e-61 | 3.725096e-58 |
| MsG0580024059.01 | MsG0580024540.01 | 0.868954 | 4.448005e-66 | 1.639410e-62 |
| MsG0580024059.01 | MsG0580024738.01 | 0.829778 | 4.254864e-55 | 4.398783e-52 |
| MsG0580024059.01 | MsG0580025750.01 | 0.805736 | 1.155907e-49 | 6.162793e-47 |
| MsG0580024059.01 | MsG0580030073.01 | 0.803052 | 4.190827e-49 | 2.085128e-46 |
| MsG0580024059.01 | MsG0780036015.01 | 0.809352 | 1.974078e-50 | 1.156453e-47 |
| MsG0580024059.01 | MsG0780037290.01 | 0.843630 | 1.245368e-58 | 1.960521e-55 |
| MsG0580024059.01 | MsG0780037894.01 | 0.813412 | 2.593855e-51 | 1.692761e-48 |
| MsG0580024059.01 | MsG0780038383.01 | 0.904047 | 1.844859e-79 | 2.803025e-75 |
| MsG0580024059.01 | MsG0780039633.01 | 0.873721 | 1.180990e-67 | 5.177548e-64 |
| MsG0580024059.01 | MsG0780040782.01 | 0.878347 | 3.023877e-69 | 1.573560e-65 |
| MsG0580024059.01 | MsG0780040900.01 | 0.800848 | 1.188588e-48 | 5.592418e-46 |
| MsG0580024059.01 | MsG0780041461.01 | 0.811741 | 6.014468e-51 | 3.752915e-48 |
| MsG0580024059.01 | MsG0780041522.01 | 0.880384 | 5.737069e-70 | 3.225879e-66 |
| MsG0580024059.01 | MsG0780041790.01 | 0.849555 | 2.998916e-60 | 5.706233e-57 |
| MsG0580024059.01 | MsG0880042125.01 | 0.824710 | 6.964637e-54 | 6.218343e-51 |
| MsG0580024059.01 | MsG0880042517.01 | 0.819124 | 1.369072e-52 | 1.044405e-49 |
| MsG0580024059.01 | MsG0880043416.01 | 0.832153 | 1.111750e-55 | 1.232097e-52 |
| MsG0580024059.01 | MsG0880046220.01 | 0.863887 | 1.806208e-64 | 5.561991e-61 |
| MsG0580024059.01 | MsG0880046486.01 | 0.830510 | 2.820267e-55 | 2.977444e-52 |
| MsG0580024059.01 | MsG0880046879.01 | 0.815340 | 9.721948e-52 | 6.684792e-49 |
| MsG0380015857.01 | MsG0580024059.01 | 0.848831 | 4.769267e-60 | 8.865032e-57 |
| MsG0380016607.01 | MsG0580024059.01 | 0.852783 | 3.679411e-61 | 7.775554e-58 |
| MsG0380016678.01 | MsG0580024059.01 | 0.808421 | 3.122300e-50 | 1.784514e-47 |
| MsG0380016760.01 | MsG0580024059.01 | 0.857903 | 1.188417e-62 | 2.979293e-59 |
| MsG0480018164.01 | MsG0580024059.01 | 0.845064 | 5.125899e-59 | 8.440764e-56 |
| MsG0280006629.01 | MsG0580024059.01 | 0.839311 | 1.709592e-57 | 2.351693e-54 |
| MsG0280006664.01 | MsG0580024059.01 | 0.831320 | 1.784996e-55 | 1.930202e-52 |
| MsG0280006747.01 | MsG0580024059.01 | 0.837298 | 5.644113e-57 | 7.299578e-54 |
| MsG0280007055.01 | MsG0580024059.01 | 0.880630 | 4.684926e-70 | 2.660926e-66 |
| MsG0280007995.01 | MsG0580024059.01 | 0.879324 | 1.367666e-69 | 7.383401e-66 |
| MsG0280009166.01 | MsG0580024059.01 | 0.828077 | 1.098756e-54 | 1.080710e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580024059.01 | MsG0180005772.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024059.01.T01 | MTR_5g005130 | 100.000 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 2.73e-36 | 115 |
| MsG0580024059.01.T01 | MTR_3g115330 | 100.000 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 2.73e-36 | 115 |
| MsG0580024059.01.T01 | MTR_1g106755 | 100.000 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 2.73e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024059.01.T01 | AT4G33865 | 92.593 | 54 | 4 | 0 | 1 | 54 | 1 | 54 | 1.86e-32 | 106 |
| MsG0580024059.01.T01 | AT3G44010 | 92.593 | 54 | 4 | 0 | 1 | 54 | 1 | 54 | 1.86e-32 | 106 |
| MsG0580024059.01.T01 | AT3G43980 | 92.593 | 54 | 4 | 0 | 1 | 54 | 1 | 54 | 1.86e-32 | 106 |
| MsG0580024059.01.T01 | AT3G44010 | 92.593 | 54 | 4 | 0 | 1 | 54 | 1 | 54 | 1.86e-32 | 106 |
Find 27 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCAATGCCAAGGAAAT+TGG | 0.216340 | 5:+348926 | MsG0580024059.01.T01:CDS |
| CAAGGAAATTGGCTTCATTA+AGG | 0.318093 | 5:+348937 | MsG0580024059.01.T01:CDS |
| TCATGGGTCACTCCAACGTT+TGG | 0.416385 | 5:+348200 | MsG0580024059.01.T01:exon |
| GAACCAGGACCATAGGTCTT+GGG | 0.430692 | 5:-348232 | None:intergenic |
| CTTAATGAAGCCAATTTCCT+TGG | 0.435363 | 5:-348936 | None:intergenic |
| ACCCATGATGATTTCTTCCT+CGG | 0.445681 | 5:-348185 | None:intergenic |
| AGAACCAGGACCATAGGTCT+TGG | 0.460388 | 5:-348233 | None:intergenic |
| GAAACTCTCATGGATTGATC+AGG | 0.487816 | 5:+348870 | MsG0580024059.01.T01:CDS |
| CCATACCATGTGCGAGAACC+AGG | 0.488541 | 5:-348247 | None:intergenic |
| CACCCCAAGACCTATGGTCC+TGG | 0.491604 | 5:+348229 | MsG0580024059.01.T01:CDS |
| AGTACGGACTCATGTGTTGC+AGG | 0.493102 | 5:+348894 | MsG0580024059.01.T01:CDS |
| AACTCTCACCCCAAGACCTA+TGG | 0.505813 | 5:+348223 | MsG0580024059.01.T01:CDS |
| CGTGTCTGTGGAAACTCTCA+TGG | 0.528677 | 5:+348860 | MsG0580024059.01.T01:CDS |
| ATGATTTCTTCCTCGGCGGT+AGG | 0.562131 | 5:-348178 | None:intergenic |
| ATGGTCCTGGTTCTCGCACA+TGG | 0.572066 | 5:+348242 | MsG0580024059.01.T01:CDS |
| CATGGATTGATCAGGAAGTA+CGG | 0.572937 | 5:+348878 | MsG0580024059.01.T01:CDS |
| CGCCGAGGAAGAAATCATCA+TGG | 0.584765 | 5:+348183 | MsG0580024059.01.T01:exon |
| TGTGCGAGAACCAGGACCAT+AGG | 0.585453 | 5:-348239 | None:intergenic |
| ATTTCTTCCTCGGCGGTAGG+CGG | 0.586413 | 5:-348175 | None:intergenic |
| CAATTTCCTTGGCATTGCTG+TGG | 0.593734 | 5:-348925 | None:intergenic |
| AACCAGGACCATAGGTCTTG+GGG | 0.607026 | 5:-348231 | None:intergenic |
| GTGCTTCCACAGCAATGCCA+AGG | 0.621677 | 5:+348919 | MsG0580024059.01.T01:CDS |
| CATGAGAGTTTCCACAGACA+CGG | 0.630579 | 5:-348859 | None:intergenic |
| GCCGAGGAAGAAATCATCAT+GGG | 0.630683 | 5:+348184 | MsG0580024059.01.T01:exon |
| GGGGTGAGAGTTCCAAACGT+TGG | 0.647246 | 5:-348212 | None:intergenic |
| CATGATGATTTCTTCCTCGG+CGG | 0.651824 | 5:-348182 | None:intergenic |
| GAGATCGCCGCCTACCGCCG+AGG | 0.653515 | 5:+348168 | MsG0580024059.01.T01:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACGTTAAAAACAAAGAAT+GGG | - | Chr5:348650-348669 | None:intergenic | 20.0% |
| !! | GTAAAATGATGTGAAAATTA+AGG | - | Chr5:348603-348622 | None:intergenic | 20.0% |
| !! | TGATTTACTGAAACAAATTT+CGG | + | Chr5:348408-348427 | MsG0580024059.01.T01:intron | 20.0% |
| !!! | TCCTACTTTGAAAATAAAAT+GGG | + | Chr5:348568-348587 | MsG0580024059.01.T01:intron | 20.0% |
| ! | CAAACGTTAAAAACAAAGAA+TGG | - | Chr5:348651-348670 | None:intergenic | 25.0% |
| ! | TCATCCAATTAACAATAAGT+TGG | - | Chr5:348391-348410 | None:intergenic | 25.0% |
| !! | GTCCTACTTTGAAAATAAAA+TGG | + | Chr5:348567-348586 | MsG0580024059.01.T01:intron | 25.0% |
| !!! | TCCCATTTTATTTTCAAAGT+AGG | - | Chr5:348572-348591 | None:intergenic | 25.0% |
| !!! | TTGTTTTTAACGTTTGTACT+TGG | + | Chr5:348655-348674 | MsG0580024059.01.T01:intron | 25.0% |
| CACACCAACTTATTGTTAAT+TGG | + | Chr5:348384-348403 | MsG0580024059.01.T01:intron | 30.0% | |
| GAACACAGTAATCAGAAATA+CGG | - | Chr5:348326-348345 | None:intergenic | 30.0% | |
| TGATGAATGACAGTAATCTA+TGG | - | Chr5:348366-348385 | None:intergenic | 30.0% | |
| ! | CATTAAGGTGAATATTTTGC+TGG | + | Chr5:348952-348971 | MsG0580024059.01.T01:CDS | 30.0% |
| AAGCACATCGAAAAATGCAT+TGG | - | Chr5:348487-348506 | None:intergenic | 35.0% | |
| CTTAATGAAGCCAATTTCCT+TGG | - | Chr5:348939-348958 | None:intergenic | 35.0% | |
| GAGTTGGATCTGAATGAATT+AGG | - | Chr5:348453-348472 | None:intergenic | 35.0% | |
| GGATCTGAATGAATTAGGAT+CGG | - | Chr5:348448-348467 | None:intergenic | 35.0% | |
| ! | ATTGGTTTCAAGAGTTGAGT+TGG | - | Chr5:348469-348488 | None:intergenic | 35.0% |
| ! | CAAGGAAATTGGCTTCATTA+AGG | + | Chr5:348937-348956 | MsG0580024059.01.T01:CDS | 35.0% |
| ACCCATGATGATTTCTTCCT+CGG | - | Chr5:348188-348207 | None:intergenic | 40.0% | |
| ACGGATCGAAGAATCTTACA+AGG | - | Chr5:348307-348326 | None:intergenic | 40.0% | |
| CGGATCGAAGAATCTTACAA+GGG | - | Chr5:348306-348325 | None:intergenic | 40.0% | |
| TCGAAGAATCTTACAAGGGT+TGG | - | Chr5:348302-348321 | None:intergenic | 40.0% | |
| ! | CATGGATTGATCAGGAAGTA+CGG | + | Chr5:348878-348897 | MsG0580024059.01.T01:CDS | 40.0% |
| ! | GAAACTCTCATGGATTGATC+AGG | + | Chr5:348870-348889 | MsG0580024059.01.T01:CDS | 40.0% |
| ! | TCGATGTGCTTGTACTTTTC+TGG | + | Chr5:348496-348515 | MsG0580024059.01.T01:intron | 40.0% |
| !! | GGTGAATATTTTGCTGGTCT+AGG | + | Chr5:348958-348977 | MsG0580024059.01.T01:CDS | 40.0% |
| CAATTTCCTTGGCATTGCTG+TGG | - | Chr5:348928-348947 | None:intergenic | 45.0% | |
| CACAGCAATGCCAAGGAAAT+TGG | + | Chr5:348926-348945 | MsG0580024059.01.T01:CDS | 45.0% | |
| CATGAGAGTTTCCACAGACA+CGG | - | Chr5:348862-348881 | None:intergenic | 45.0% | |
| CATGATGATTTCTTCCTCGG+CGG | - | Chr5:348185-348204 | None:intergenic | 45.0% | |
| GCCGAGGAAGAAATCATCAT+GGG | + | Chr5:348184-348203 | MsG0580024059.01.T01:exon | 45.0% | |
| AACCAGGACCATAGGTCTTG+GGG | - | Chr5:348234-348253 | None:intergenic | 50.0% | |
| AACTCTCACCCCAAGACCTA+TGG | + | Chr5:348223-348242 | MsG0580024059.01.T01:CDS | 50.0% | |
| ACAGACACGGCTACAAAACG+CGG | - | Chr5:348849-348868 | None:intergenic | 50.0% | |
| AGAACCAGGACCATAGGTCT+TGG | - | Chr5:348236-348255 | None:intergenic | 50.0% | |
| AGTACGGACTCATGTGTTGC+AGG | + | Chr5:348894-348913 | MsG0580024059.01.T01:CDS | 50.0% | |
| ATGATTTCTTCCTCGGCGGT+AGG | - | Chr5:348181-348200 | None:intergenic | 50.0% | |
| CGCCGAGGAAGAAATCATCA+TGG | + | Chr5:348183-348202 | MsG0580024059.01.T01:exon | 50.0% | |
| CGTGTCTGTGGAAACTCTCA+TGG | + | Chr5:348860-348879 | MsG0580024059.01.T01:CDS | 50.0% | |
| GAACCAGGACCATAGGTCTT+GGG | - | Chr5:348235-348254 | None:intergenic | 50.0% | |
| TCATGGGTCACTCCAACGTT+TGG | + | Chr5:348200-348219 | MsG0580024059.01.T01:exon | 50.0% | |
| ATTTCTTCCTCGGCGGTAGG+CGG | - | Chr5:348178-348197 | None:intergenic | 55.0% | |
| CCATACCATGTGCGAGAACC+AGG | - | Chr5:348250-348269 | None:intergenic | 55.0% | |
| GGGGTGAGAGTTCCAAACGT+TGG | - | Chr5:348215-348234 | None:intergenic | 55.0% | |
| GTGCTTCCACAGCAATGCCA+AGG | + | Chr5:348919-348938 | MsG0580024059.01.T01:CDS | 55.0% | |
| TGTGCGAGAACCAGGACCAT+AGG | - | Chr5:348242-348261 | None:intergenic | 55.0% | |
| ! | ATGGTCCTGGTTCTCGCACA+TGG | + | Chr5:348242-348261 | MsG0580024059.01.T01:CDS | 55.0% |
| ! | CCTGGTTCTCGCACATGGTA+TGG | + | Chr5:348247-348266 | MsG0580024059.01.T01:intron | 55.0% |
| ! | GCGTTTTGTAGCCGTGTCTG+TGG | + | Chr5:348848-348867 | MsG0580024059.01.T01:intron | 55.0% |
| CACCCCAAGACCTATGGTCC+TGG | + | Chr5:348229-348248 | MsG0580024059.01.T01:CDS | 60.0% | |
| TGCTGCTGCTGCTGCTGCTA+GGG | + | Chr5:348142-348161 | MsG0580024059.01.T01:five_prime_UTR | 60.0% | |
| TTGCTGCTGCTGCTGCTGCT+AGG | + | Chr5:348141-348160 | MsG0580024059.01.T01:five_prime_UTR | 60.0% | |
| GAGATCGCCGCCTACCGCCG+AGG | + | Chr5:348168-348187 | MsG0580024059.01.T01:five_prime_UTR | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 348119 | 348979 | 348119 | ID=MsG0580024059.01;Name=MsG0580024059.01 |
| Chr5 | mRNA | 348119 | 348979 | 348119 | ID=MsG0580024059.01.T01;Parent=MsG0580024059.01;Name=MsG0580024059.01.T01;_AED=0.41;_eAED=0.45;_QI=83|1|0.5|1|0|0|2|0|60 |
| Chr5 | exon | 348119 | 348263 | 348119 | ID=MsG0580024059.01.T01:exon:28;Parent=MsG0580024059.01.T01 |
| Chr5 | exon | 348859 | 348979 | 348859 | ID=MsG0580024059.01.T01:exon:29;Parent=MsG0580024059.01.T01 |
| Chr5 | five_prime_UTR | 348119 | 348201 | 348119 | ID=MsG0580024059.01.T01:five_prime_utr;Parent=MsG0580024059.01.T01 |
| Chr5 | CDS | 348202 | 348263 | 348202 | ID=MsG0580024059.01.T01:cds;Parent=MsG0580024059.01.T01 |
| Chr5 | CDS | 348859 | 348979 | 348859 | ID=MsG0580024059.01.T01:cds;Parent=MsG0580024059.01.T01 |
| Gene Sequence |
| Protein sequence |
