AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005860.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005860.01.T01 MTR_3g045700 70.526 95 25 1 1 92 256 350 3.46e-39 133
MsG0180005860.01.T01 MTR_1g109520 67.347 98 25 1 1 91 79 176 7.37e-39 127
MsG0180005860.01.T01 MTR_7g083950 65.263 95 30 1 1 92 338 432 5.69e-36 126
MsG0180005860.01.T01 MTR_1g109340 67.021 94 28 1 1 91 261 354 3.97e-35 122
MsG0180005860.01.T01 MTR_1g100280 62.887 97 31 2 1 92 241 337 9.00e-35 121
MsG0180005860.01.T01 MTR_7g070100 57.292 96 36 2 1 92 256 350 1.21e-27 102
MsG0180005860.01.T01 MTR_4g133320 55.914 93 40 1 1 92 216 308 4.77e-27 100
MsG0180005860.01.T01 MTR_3g006100 54.737 95 40 1 1 92 258 352 6.57e-27 100
MsG0180005860.01.T01 MTR_4g133490 54.839 93 41 1 1 92 256 348 1.92e-26 99.8
MsG0180005860.01.T01 MTR_3g006010 54.348 92 39 1 1 89 375 466 3.80e-26 100
MsG0180005860.01.T01 MTR_3g006180 53.191 94 41 1 1 91 245 338 5.04e-25 95.9
MsG0180005860.01.T01 MTR_3g006120 52.174 92 41 1 1 89 258 349 1.45e-23 92.0
MsG0180005860.01.T01 MTR_3g006140 54.737 95 36 4 2 91 281 373 2.36e-22 89.0
MsG0180005860.01.T01 MTR_6g091850 43.564 101 48 2 1 92 1 101 5.87e-22 82.4
MsG0180005860.01.T01 MTR_3g068220 47.475 99 43 2 1 90 257 355 7.36e-22 87.4
MsG0180005860.01.T01 MTR_6g011110 47.619 105 39 3 1 92 176 277 1.04e-21 85.9
MsG0180005860.01.T01 MTR_6g088340 45.545 101 46 2 1 92 333 433 1.29e-21 87.4
MsG0180005860.01.T01 MTR_6g091830 46.535 101 45 2 1 92 355 455 3.18e-21 86.3
MsG0180005860.01.T01 MTR_6g092140 45.545 101 46 2 1 92 355 455 1.20e-20 84.7
MsG0180005860.01.T01 MTR_3g086480 48.276 87 41 2 1 84 168 253 2.92e-19 79.3
MsG0180005860.01.T01 MTR_1g089620 76.923 39 9 0 1 39 110 148 7.58e-15 67.8
MsG0180005860.01.T01 MTR_3g085910 50.000 58 29 0 1 58 218 275 1.15e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCATATCCCCACCTGTT+GGG 0.307053 1:+96962879 None:intergenic
TTCCAGTTTGACGTAATTCA+TGG 0.341057 1:+96962962 None:intergenic
TTCAAGTTGTAGTGTGTATT+CGG 0.358079 1:-96962826 MsG0180005860.01.T01:CDS
AACCATGAATTACGTCAAAC+TGG 0.393600 1:-96962964 None:intergenic
TTACGTCAAACTGGAAACTT+TGG 0.400584 1:-96962955 MsG0180005860.01.T01:CDS
AAAACACCAATCAGACTTGA+AGG 0.428047 1:+96962746 None:intergenic
CAAGTTCCTTCAAGTCTGAT+TGG 0.438389 1:-96962752 MsG0180005860.01.T01:CDS
ATGCATTGAGCAACCCCAAC+AGG 0.449427 1:-96962893 MsG0180005860.01.T01:CDS
TACGTCAAACTGGAAACTTT+GGG 0.462690 1:-96962954 MsG0180005860.01.T01:CDS
ATTACACTTTCAACTCCTCA+TGG 0.500145 1:-96962777 MsG0180005860.01.T01:CDS
GCTTTCATATCCCCACCTGT+TGG 0.512470 1:+96962878 None:intergenic
TTTCATATCCCCACCTGTTG+GGG 0.516850 1:+96962880 None:intergenic
AGTTGTAGTGTGTATTCGGT+TGG 0.533748 1:-96962822 MsG0180005860.01.T01:CDS
CATTGAGCAACCCCAACAGG+TGG 0.595743 1:-96962890 MsG0180005860.01.T01:CDS
ATTGAGCAACCCCAACAGGT+GGG 0.636644 1:-96962889 MsG0180005860.01.T01:CDS
TTGAGCAACCCCAACAGGTG+GGG 0.661540 1:-96962888 MsG0180005860.01.T01:CDS
TTGAAGGAACTTGTTCCATG+AGG 0.687755 1:+96962762 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTAGACATCAACATTTTG+AGG - Chr1:96962937-96962956 MsG0180005860.01.T01:CDS 25.0%
!! TTCAAGTTGTAGTGTGTATT+CGG - Chr1:96962838-96962857 MsG0180005860.01.T01:CDS 30.0%
AAAACACCAATCAGACTTGA+AGG + Chr1:96962921-96962940 None:intergenic 35.0%
ATTACACTTTCAACTCCTCA+TGG - Chr1:96962887-96962906 MsG0180005860.01.T01:CDS 35.0%
TACGTCAAACTGGAAACTTT+GGG - Chr1:96962710-96962729 MsG0180005860.01.T01:CDS 35.0%
TTACGTCAAACTGGAAACTT+TGG - Chr1:96962709-96962728 MsG0180005860.01.T01:CDS 35.0%
AGTTGTAGTGTGTATTCGGT+TGG - Chr1:96962842-96962861 MsG0180005860.01.T01:CDS 40.0%
CAAGTTCCTTCAAGTCTGAT+TGG - Chr1:96962912-96962931 MsG0180005860.01.T01:CDS 40.0%
! TTGAAGGAACTTGTTCCATG+AGG + Chr1:96962905-96962924 None:intergenic 40.0%
CTTTCATATCCCCACCTGTT+GGG + Chr1:96962788-96962807 None:intergenic 45.0%
TTTCATATCCCCACCTGTTG+GGG + Chr1:96962787-96962806 None:intergenic 45.0%
ATGCATTGAGCAACCCCAAC+AGG - Chr1:96962771-96962790 MsG0180005860.01.T01:CDS 50.0%
ATTGAGCAACCCCAACAGGT+GGG - Chr1:96962775-96962794 MsG0180005860.01.T01:CDS 50.0%
GCTTTCATATCCCCACCTGT+TGG + Chr1:96962789-96962808 None:intergenic 50.0%
CATTGAGCAACCCCAACAGG+TGG - Chr1:96962774-96962793 MsG0180005860.01.T01:CDS 55.0%
TTGAGCAACCCCAACAGGTG+GGG - Chr1:96962776-96962795 MsG0180005860.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 96962704 96962982 96962704 ID=MsG0180005860.01;Name=MsG0180005860.01
Chr1 mRNA 96962704 96962982 96962704 ID=MsG0180005860.01.T01;Parent=MsG0180005860.01;Name=MsG0180005860.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|92
Chr1 exon 96962704 96962982 96962704 ID=MsG0180005860.01.T01:exon:30698;Parent=MsG0180005860.01.T01
Chr1 CDS 96962704 96962982 96962704 ID=MsG0180005860.01.T01:cds;Parent=MsG0180005860.01.T01
Gene Sequence

>MsG0180005860.01.T01

ATGAATTACGTCAAACTGGAAACTTTGGGCGACATTGCATTATTTTATGTTTCATACAGAAGTTGTTATGCATTGAGCAACCCCAACAGGTGGGGATATGAAAGCAACTCTGTGTATATCATCAGTTCTCTATCTTCAAGTTGTAGTGTGTATTCGGTTGGTGATAAAAAATTGCAAAAGAACATTACACTTTCAACTCCTCATGGAACAAGTTCCTTCAAGTCTGATTGGTGTTTTAGACATCAACATTTTGAGGTAGATTATTCTCTAGTTGAGTGA

Protein sequence

>MsG0180005860.01.T01

MNYVKLETLGDIALFYVSYRSCYALSNPNRWGYESNSVYIISSLSSSCSVYSVGDKKLQKNITLSTPHGTSSFKSDWCFRHQHFEVDYSLVE*