AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026237.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026237.01.T01 MTR_5g042520 89.179 268 12 3 1 254 1 265 1.26e-165 459
MsG0580026237.01.T01 MTR_5g042520 86.154 195 10 3 1 181 1 192 2.10e-107 312
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026237.01.T01 AT1G13990 56.055 289 76 6 1 252 1 275 2.28e-87 261
MsG0580026237.01.T01 AT1G13990 51.420 317 75 6 1 252 1 303 8.72e-83 250
MsG0580026237.01.T01 AT1G13990 45.312 320 93 9 1 252 1 306 6.56e-58 187

Find 91 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTTCCCTTTGGCTGTTT+TGG 0.249654 5:+32965357 None:intergenic
CAGATTAAGCTTAAGGCTTT+CGG 0.274485 5:+32965480 None:intergenic
AAGAACTCATCCGAGTTTCC+AGG 0.333604 5:+32968958 None:intergenic
AGATTAAGCTTAAGGCTTTC+GGG 0.343222 5:+32965481 None:intergenic
GTGGCGACGGAAAGGGTTGG+TGG 0.357740 5:+32969164 None:intergenic
CCTTTCTCCTCCTCCTTGTT+CGG 0.360594 5:+32969079 None:intergenic
GTCGTCGGCGGCGGCGGCAG+AGG 0.365376 5:-32969042 MsG0580026237.01.T01:CDS
GAGCTCGCAGATTAAGCTTA+AGG 0.373495 5:+32965473 None:intergenic
TGCAACGTCGTCGGCGGCGG+CGG 0.376584 5:-32969048 MsG0580026237.01.T01:CDS
AATCACACCTTATATTTCTC+CGG 0.387380 5:+32966342 None:intergenic
TGAAAACCCGGAGAAATATA+AGG 0.401206 5:-32966349 MsG0580026237.01.T01:intron
AGAGAGAGAGGCGGCGGGTG+TGG 0.404308 5:+32969145 None:intergenic
AGCTTAAGGCTTTCGGGTAC+AGG 0.405348 5:+32965487 None:intergenic
TCAGATCATCTTGATCTTCA+AGG 0.424973 5:+32965440 None:intergenic
CTAATATCTTCTACTTGCAT+TGG 0.426258 5:+32966147 None:intergenic
CGACGGAAAGGGTTGGTGGG+AGG 0.428198 5:+32969168 None:intergenic
TTGATCTTTGCAAGAAAATG+AGG 0.433526 5:-32965546 MsG0580026237.01.T01:CDS
CGGCGGGTGTGGCGACGGAA+AGG 0.455266 5:+32969156 None:intergenic
TACCGATGAGGCGTCTATCT+CGG 0.460916 5:+32965657 None:intergenic
TCGTGCAACGTCGTCGGCGG+CGG 0.462296 5:-32969051 MsG0580026237.01.T01:CDS
GGCTTTGAAGTTTCTCTCCA+AGG 0.462303 5:+32966413 None:intergenic
TTAATCTGCGAGCTCGAAAA+CGG 0.468199 5:-32965465 MsG0580026237.01.T01:CDS
GACGGAAAGGGTTGGTGGGA+GGG 0.471223 5:+32969169 None:intergenic
GGCGGGTGTGGCGACGGAAA+GGG 0.478572 5:+32969157 None:intergenic
GGAGAAGAGAGAGAGGCGGC+GGG 0.484370 5:+32969140 None:intergenic
CAAGTAGAAGATATTAGAGA+TGG 0.493706 5:-32966141 MsG0580026237.01.T01:CDS
GGGAGAAGAGAGAGAGGCGG+CGG 0.493916 5:+32969139 None:intergenic
AGAGGCGGCGGGTGTGGCGA+CGG 0.499779 5:+32969151 None:intergenic
CAGTGGAAACGAGGAGGAGG+TGG 0.500190 5:-32969010 MsG0580026237.01.T01:CDS
GAGGTGTAGAGATGAGTCTC+GGG 0.508797 5:+32969106 None:intergenic
GTCGGCGGCGGCGGCAGAGG+AGG 0.511472 5:-32969039 MsG0580026237.01.T01:CDS
AGCGAAGTTGAAAGAGAGCT+TGG 0.516832 5:-32966249 MsG0580026237.01.T01:CDS
GCTTTGAAGTTTCTCTCCAA+GGG 0.519722 5:+32966414 None:intergenic
AAATCAGTCTAAACAAGCAA+GGG 0.520422 5:-32966184 MsG0580026237.01.T01:CDS
GAAATTCAACTCCCTTGTGA+TGG 0.520817 5:-32965414 MsG0580026237.01.T01:three_prime_UTR
GTTGGTGGGAGGGAGAGCGA+TGG 0.522907 5:+32969179 None:intergenic
AGATATTAGAGATGGAGTGC+TGG 0.526832 5:-32966133 MsG0580026237.01.T01:intron
CTCCTCCTTGTTCGGAAGTG+AGG 0.531784 5:+32969087 None:intergenic
CCGAACAAGGAGGAGGAGAA+AGG 0.536819 5:-32969079 MsG0580026237.01.T01:CDS
ATCACACCTTATATTTCTCC+GGG 0.540391 5:+32966343 None:intergenic
TGTGTATGTACTCACCCAAA+AGG 0.546154 5:-32966390 MsG0580026237.01.T01:CDS
TGATCTTTGCAAGAAAATGA+GGG 0.547590 5:-32965545 MsG0580026237.01.T01:CDS
GAAGTATCGTGCAACGTCGT+CGG 0.549545 5:-32969057 MsG0580026237.01.T01:CDS
CTACACCTCACTTCCGAACA+AGG 0.552595 5:-32969092 MsG0580026237.01.T01:CDS
GATGGCGGGAGAAGAGAGAG+AGG 0.554072 5:+32969133 None:intergenic
GTGGGAGGGAGAGCGATGGA+AGG 0.556169 5:+32969183 None:intergenic
AAATTCAACTCCCTTGTGAT+GGG 0.557810 5:-32965413 MsG0580026237.01.T01:three_prime_UTR
TGACAGTGGAAACGAGGAGG+AGG 0.559489 5:-32969013 MsG0580026237.01.T01:CDS
AAAATCAGTCTAAACAAGCA+AGG 0.561023 5:-32966185 MsG0580026237.01.T01:CDS
GAGAAAGCGCTTCACATGGA+TGG 0.561227 5:-32968988 MsG0580026237.01.T01:CDS
CAGATCATCTTGATCTTCAA+GGG 0.562330 5:+32965441 None:intergenic
CAGTTCTATTCCGCCGAGGT+AGG 0.562349 5:-32965516 MsG0580026237.01.T01:CDS
CTTGCTGCAAGGAGGGTGTG+AGG 0.562675 5:-32965686 MsG0580026237.01.T01:CDS
TGAGGTGTAGAGATGAGTCT+CGG 0.563676 5:+32969105 None:intergenic
CGATGGAAGGTGAAACTGAA+GGG 0.566266 5:+32969196 None:intergenic
TTGCTGCAAGGAGGGTGTGA+GGG 0.569430 5:-32965685 MsG0580026237.01.T01:CDS
TGGCGACGGAAAGGGTTGGT+GGG 0.569624 5:+32969165 None:intergenic
GTCGCTTCTTTCCCATCACA+AGG 0.572178 5:+32965402 None:intergenic
CTTGGTTATTTGTACCGATG+AGG 0.573244 5:+32965645 None:intergenic
TGCCGAGATAGACGCCTCAT+CGG 0.575782 5:-32965659 MsG0580026237.01.T01:intron
AACTGAAGGGAGAGAATCCA+TGG 0.580578 5:+32969209 None:intergenic
AGTCTAAACAAGCAAGGGGA+GGG 0.591602 5:-32966179 MsG0580026237.01.T01:CDS
CTCACTTCCGAACAAGGAGG+AGG 0.592127 5:-32969086 MsG0580026237.01.T01:CDS
TAACACCCAAAACAGCCAAA+GGG 0.603277 5:-32965363 MsG0580026237.01.T01:three_prime_UTR
GTACAGGAGGTGTCCTACCT+CGG 0.604704 5:+32965503 None:intergenic
CAGTCTAAACAAGCAAGGGG+AGG 0.606675 5:-32966180 MsG0580026237.01.T01:CDS
AGGAGATGACAGTGGAAACG+AGG 0.607798 5:-32969019 MsG0580026237.01.T01:CDS
AATTGTGCACGATGAAAACC+CGG 0.610498 5:-32966361 MsG0580026237.01.T01:CDS
GGTGGAGAAAGCGCTTCACA+TGG 0.611542 5:-32968992 MsG0580026237.01.T01:CDS
GTATCGTGCAACGTCGTCGG+CGG 0.612057 5:-32969054 MsG0580026237.01.T01:CDS
GTAACACCCAAAACAGCCAA+AGG 0.614205 5:-32965364 MsG0580026237.01.T01:three_prime_UTR
GGTGTGGCGACGGAAAGGGT+TGG 0.615753 5:+32969161 None:intergenic
GGCGGGAGAAGAGAGAGAGG+CGG 0.617937 5:+32969136 None:intergenic
TTAAGGCTTTCGGGTACAGG+AGG 0.618037 5:+32965490 None:intergenic
AAGTATTGTGACTTGCTGCA+AGG 0.620712 5:-32965697 MsG0580026237.01.T01:CDS
CGTACGCATGTCATAAGCAC+GGG 0.624471 5:+32968921 None:intergenic
GCGATGGAAGGTGAAACTGA+AGG 0.640575 5:+32969195 None:intergenic
ATTGTGACTTGCTGCAAGGA+GGG 0.645627 5:-32965693 MsG0580026237.01.T01:CDS
AGATGACAGTGGAAACGAGG+AGG 0.659545 5:-32969016 MsG0580026237.01.T01:CDS
CTTGCAGTTCTATTCCGCCG+AGG 0.660240 5:-32965520 MsG0580026237.01.T01:CDS
AATCAGTCTAAACAAGCAAG+GGG 0.668776 5:-32966183 MsG0580026237.01.T01:CDS
ACGTACGCATGTCATAAGCA+CGG 0.670371 5:+32968920 None:intergenic
AGGTGTAGAGATGAGTCTCG+GGG 0.671225 5:+32969107 None:intergenic
ACGCATGTCATAAGCACGGG+AGG 0.671857 5:+32968924 None:intergenic
TCGCTTCTTTCCCATCACAA+GGG 0.698146 5:+32965403 None:intergenic
CAGGAGGTGTCCTACCTCGG+CGG 0.706198 5:+32965506 None:intergenic
TATTGTGACTTGCTGCAAGG+AGG 0.715644 5:-32965694 MsG0580026237.01.T01:CDS
CACCTCACTTCCGAACAAGG+AGG 0.734423 5:-32969089 MsG0580026237.01.T01:CDS
GGCAGAGGAGGAGATGACAG+TGG 0.737489 5:-32969027 MsG0580026237.01.T01:CDS
GGTATTTGAAGATGAAAACG+AGG 0.756977 5:-32965725 MsG0580026237.01.T01:CDS
GGTGTAGAGATGAGTCTCGG+GGG 0.766311 5:+32969108 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATCATTTAACAATT+TGG - Chr5:32968706-32968725 MsG0580026237.01.T01:intron 10.0%
!! ATTATATTATGTTTAGATAT+TGG - Chr5:32967783-32967802 MsG0580026237.01.T01:intron 10.0%
!!! AATTTAATTTTTTGTTTTCA+TGG - Chr5:32965652-32965671 MsG0580026237.01.T01:intron 10.0%
!!! ATAAATTTAGAATGAAATTT+AGG + Chr5:32966098-32966117 None:intergenic 10.0%
!!! TTAAGCTATTTTTATAAAAA+AGG + Chr5:32965913-32965932 None:intergenic 10.0%
!! ATAATAACAACTAAGTAATA+TGG + Chr5:32967605-32967624 None:intergenic 15.0%
!! ATTATTTACCTGTTTATTTA+GGG - Chr5:32967635-32967654 MsG0580026237.01.T01:intron 15.0%
!! TATTATTTACCTGTTTATTT+AGG - Chr5:32967634-32967653 MsG0580026237.01.T01:intron 15.0%
!!! TATTGTATTCTGTTTTATTT+TGG - Chr5:32967299-32967318 MsG0580026237.01.T01:intron 15.0%
!! AAAAACAATAAACTGACAAA+AGG + Chr5:32967969-32967988 None:intergenic 20.0%
!! AAAAGAATAAAGAAAGGAAT+TGG - Chr5:32966252-32966271 MsG0580026237.01.T01:intron 20.0%
!! AGCAATAAATTAAACTAAGA+TGG + Chr5:32967382-32967401 None:intergenic 20.0%
!! AGTAACTATATACTACAAAT+AGG + Chr5:32966346-32966365 None:intergenic 20.0%
!! ATATCGTTTGTGAATAAATT+CGG - Chr5:32966720-32966739 MsG0580026237.01.T01:intron 20.0%
!! ATTTCACATTACAAAATGTT+TGG + Chr5:32968427-32968446 None:intergenic 20.0%
!! CTAGAAAACTTATGTAAATA+AGG + Chr5:32965834-32965853 None:intergenic 20.0%
!! TAGGAAAAAAAACAGAAAAA+AGG + Chr5:32968153-32968172 None:intergenic 20.0%
!! TATTTGGATTGAATTATTTG+AGG - Chr5:32965735-32965754 MsG0580026237.01.T01:intron 20.0%
!!! AATATATCAGAAAGTATCAA+AGG - Chr5:32968444-32968463 MsG0580026237.01.T01:intron 20.0%
!!! ATTTAAAGTCAAATTGCTTA+TGG - Chr5:32965704-32965723 MsG0580026237.01.T01:CDS 20.0%
!!! CTATCAATATTTGAAATCTT+CGG - Chr5:32968170-32968189 MsG0580026237.01.T01:intron 20.0%
!!! CTTATTTACATAAGTTTTCT+AGG - Chr5:32965832-32965851 MsG0580026237.01.T01:intron 20.0%
!!! TTGTTACTTGTAATAATTGA+AGG - Chr5:32967939-32967958 MsG0580026237.01.T01:intron 20.0%
! AATAAGGTGAAATCTATTGA+TGG + Chr5:32965818-32965837 None:intergenic 25.0%
! AGAGTGAAAAGAATAAAGAA+AGG - Chr5:32966246-32966265 MsG0580026237.01.T01:CDS 25.0%
! ATTATGTTCAAACACATTAG+AGG + Chr5:32968483-32968502 None:intergenic 25.0%
! ATTTAACAATTTGGCAATGT+TGG - Chr5:32968715-32968734 MsG0580026237.01.T01:intron 25.0%
! CATAATAAGATCAAATTCGA+TGG + Chr5:32967210-32967229 None:intergenic 25.0%
! CGAAGATTTCAAATATTGAT+AGG + Chr5:32968172-32968191 None:intergenic 25.0%
! CTAATGTGTTTGAACATAAT+TGG - Chr5:32968483-32968502 MsG0580026237.01.T01:intron 25.0%
! GAATTTATTCACAAACGATA+TGG + Chr5:32966721-32966740 None:intergenic 25.0%
! GATCTTCATGATTTAGATTA+AGG - Chr5:32965676-32965695 MsG0580026237.01.T01:CDS 25.0%
! GTATACTAAGGAAAACTAAT+CGG + Chr5:32968559-32968578 None:intergenic 25.0%
! GTTTAAATTATCAAGTAGTG+AGG + Chr5:32967167-32967186 None:intergenic 25.0%
! TAATTGGAATTTGATTGCTT+TGG - Chr5:32968499-32968518 MsG0580026237.01.T01:intron 25.0%
! TATACTAAGGAAAACTAATC+GGG + Chr5:32968558-32968577 None:intergenic 25.0%
! TTGTAAAACTGAGTTAATTG+AGG - Chr5:32966632-32966651 MsG0580026237.01.T01:intron 25.0%
! TTTACAAAAAAGAGTAGAAC+AGG - Chr5:32966500-32966519 MsG0580026237.01.T01:intron 25.0%
! TTTGGAGATTATTGTTAGAA+AGG + Chr5:32966563-32966582 None:intergenic 25.0%
!! AAATTTAGGTTAAGTTGCTT+GGG + Chr5:32966084-32966103 None:intergenic 25.0%
!! AATCATTGTTTCATTTTACC+AGG + Chr5:32968630-32968649 None:intergenic 25.0%
!!! TATTCTGTTTTATTTTGGCT+GGG - Chr5:32967304-32967323 MsG0580026237.01.T01:intron 25.0%
AAAATCAGTCTAAACAAGCA+AGG - Chr5:32968282-32968301 MsG0580026237.01.T01:intron 30.0%
AAACACTAGACCTTAATTAC+AGG + Chr5:32967677-32967696 None:intergenic 30.0%
AAATCAGTCTAAACAAGCAA+GGG - Chr5:32968283-32968302 MsG0580026237.01.T01:intron 30.0%
AAATTATCAAGTAGTGAGGA+TGG + Chr5:32967163-32967182 None:intergenic 30.0%
AACACTAGACCTTAATTACA+GGG + Chr5:32967676-32967695 None:intergenic 30.0%
AAGTCAAATTGCTTATGGTA+AGG - Chr5:32965709-32965728 MsG0580026237.01.T01:CDS 30.0%
AATCACACCTTATATTTCTC+CGG + Chr5:32968128-32968147 None:intergenic 30.0%
ACCAACAATAATGAGTATGA+TGG + Chr5:32966158-32966177 None:intergenic 30.0%
ACCATCATACTCATTATTGT+TGG - Chr5:32966154-32966173 MsG0580026237.01.T01:CDS 30.0%
AGACATGTATAAAATGACAG+CGG - Chr5:32966801-32966820 MsG0580026237.01.T01:intron 30.0%
AGTCAAATTGCTTATGGTAA+GGG - Chr5:32965710-32965729 MsG0580026237.01.T01:CDS 30.0%
ATACTAAGGAAAACTAATCG+GGG + Chr5:32968557-32968576 None:intergenic 30.0%
ATAGTCAAAGTACAATTGCT+TGG + Chr5:32968843-32968862 None:intergenic 30.0%
CAACTTCTATCAAAAGACAT+CGG - Chr5:32966412-32966431 MsG0580026237.01.T01:CDS 30.0%
CAAGTAGAAGATATTAGAGA+TGG - Chr5:32968326-32968345 MsG0580026237.01.T01:intron 30.0%
CTAATATCTTCTACTTGCAT+TGG + Chr5:32968323-32968342 None:intergenic 30.0%
GAAGTTTATAAATTGCATCC+TGG + Chr5:32968535-32968554 None:intergenic 30.0%
GAGTATCTTAGTTTATCTCT+AGG - Chr5:32967527-32967546 MsG0580026237.01.T01:intron 30.0%
GGATAAGAAAACTACTTCAA+TGG - Chr5:32966380-32966399 MsG0580026237.01.T01:CDS 30.0%
GTAATATGGCAACTAATTCT+AGG + Chr5:32967591-32967610 None:intergenic 30.0%
GTATAAACCATGCAATATGT+TGG + Chr5:32968660-32968679 None:intergenic 30.0%
GTGTTTCAAAATTCAAACTC+AGG - Chr5:32966676-32966695 MsG0580026237.01.T01:intron 30.0%
TAACTCTCAAATCACATGAT+AGG + Chr5:32967259-32967278 None:intergenic 30.0%
TGAAAACAGTTGCAAATAGT+TGG - Chr5:32966010-32966029 MsG0580026237.01.T01:intron 30.0%
TGATCTTTGCAAGAAAATGA+GGG - Chr5:32968922-32968941 MsG0580026237.01.T01:intron 30.0%
TGTAGTATATAGTTACTGAG+CGG - Chr5:32966349-32966368 MsG0580026237.01.T01:intron 30.0%
TTAGAGTAGTAGGTATACTA+AGG + Chr5:32968571-32968590 None:intergenic 30.0%
TTGAGCGAGTCTAAATATAT+TGG - Chr5:32966767-32966786 MsG0580026237.01.T01:intron 30.0%
TTGATCTTTGCAAGAAAATG+AGG - Chr5:32968921-32968940 MsG0580026237.01.T01:intron 30.0%
! AACTATTTGCAACTGTTTTC+AGG + Chr5:32966011-32966030 None:intergenic 30.0%
! AACTTACAGTTTAGAGTAGT+AGG + Chr5:32968581-32968600 None:intergenic 30.0%
! AATAGGATTTTTCGTTGTAG+AGG + Chr5:32966329-32966348 None:intergenic 30.0%
! ACTATTTGCAACTGTTTTCA+GGG + Chr5:32966010-32966029 None:intergenic 30.0%
! ATACACATTTTTGTTCCCTT+TGG + Chr5:32969122-32969141 None:intergenic 30.0%
! ATAGGATTTTTCGTTGTAGA+GGG + Chr5:32966328-32966347 None:intergenic 30.0%
! CAAACAAAGCAAAGTTGTTT+TGG + Chr5:32966581-32966600 None:intergenic 30.0%
! CTAAACTGTAAGTTTGTTCA+TGG - Chr5:32968587-32968606 MsG0580026237.01.T01:intron 30.0%
! GAAATTTAGGTTAAGTTGCT+TGG + Chr5:32966085-32966104 None:intergenic 30.0%
! TACCTGTTTATTTAGGGTTA+GGG - Chr5:32967641-32967660 MsG0580026237.01.T01:intron 30.0%
! TGTCATCTAAAACTGATGAA+AGG + Chr5:32968032-32968051 None:intergenic 30.0%
! TTACCTGTTTATTTAGGGTT+AGG - Chr5:32967640-32967659 MsG0580026237.01.T01:intron 30.0%
!! TGCTTCCTTTTGAAAACAAT+AGG + Chr5:32968244-32968263 None:intergenic 30.0%
!!! CTACTCTTTTTTGTAAACCA+AGG + Chr5:32966497-32966516 None:intergenic 30.0%
!!! CTGTCATTTTATACATGTCT+AGG + Chr5:32966801-32966820 None:intergenic 30.0%
!!! GTATTCTGTTTTATTTTGGC+TGG - Chr5:32967303-32967322 MsG0580026237.01.T01:intron 30.0%
AAACAGTTGCAAATAGTTGG+TGG - Chr5:32966013-32966032 MsG0580026237.01.T01:intron 35.0%
AAATTCAACTCCCTTGTGAT+GGG - Chr5:32969054-32969073 MsG0580026237.01.T01:CDS 35.0%
AATCAGTCTAAACAAGCAAG+GGG - Chr5:32968284-32968303 MsG0580026237.01.T01:intron 35.0%
AATCATACGAGTCGAATCTA+TGG - Chr5:32967857-32967876 MsG0580026237.01.T01:intron 35.0%
ACATATACAGATCTCCATTG+AGG + Chr5:32967893-32967912 None:intergenic 35.0%
AGATTAAGCTTAAGGCTTTC+GGG + Chr5:32968989-32969008 None:intergenic 35.0%
ATAATACTGTCGAAGTTGTG+AGG + Chr5:32967768-32967787 None:intergenic 35.0%
ATCACACCTTATATTTCTCC+GGG + Chr5:32968127-32968146 None:intergenic 35.0%
ATCATAGCTGCTAGAATCAA+AGG + Chr5:32965589-32965608 None:intergenic 35.0%
ATCATCAACTTAGCTAAGCT+CGG - Chr5:32966292-32966311 MsG0580026237.01.T01:intron 35.0%
CACTCTTGTACATCCAAATA+GGG - Chr5:32965940-32965959 MsG0580026237.01.T01:intron 35.0%
CAGATCATCTTGATCTTCAA+GGG + Chr5:32969029-32969048 None:intergenic 35.0%
CAGATTAAGCTTAAGGCTTT+CGG + Chr5:32968990-32969009 None:intergenic 35.0%
CGTTTATGCATAGAGCTATT+GGG + Chr5:32966990-32967009 None:intergenic 35.0%
CTCTAGACTAATGATATCCT+TGG - Chr5:32966477-32966496 MsG0580026237.01.T01:intron 35.0%
GAAAACTACTTCAATGGAAG+AGG - Chr5:32966386-32966405 MsG0580026237.01.T01:CDS 35.0%
GACATGTATAAAATGACAGC+GGG - Chr5:32966802-32966821 MsG0580026237.01.T01:intron 35.0%
GGACAAATTGACAAATCACA+AGG + Chr5:32967189-32967208 None:intergenic 35.0%
GGTATTTGAAGATGAAAACG+AGG - Chr5:32968742-32968761 MsG0580026237.01.T01:intron 35.0%
GTCTTGTATCCCTGTAATTA+AGG - Chr5:32967664-32967683 MsG0580026237.01.T01:intron 35.0%
TATGCAAATGTATGCAAGTG+TGG + Chr5:32966920-32966939 None:intergenic 35.0%
TCAGATCATCTTGATCTTCA+AGG + Chr5:32969030-32969049 None:intergenic 35.0%
TCCTTCACCTCATTAAAGAT+GGG + Chr5:32966960-32966979 None:intergenic 35.0%
TCGTTTATGCATAGAGCTAT+TGG + Chr5:32966991-32967010 None:intergenic 35.0%
TCTAGTGAGAAATCCCTATT+TGG + Chr5:32965956-32965975 None:intergenic 35.0%
TGAAAACCCGGAGAAATATA+AGG - Chr5:32968118-32968137 MsG0580026237.01.T01:intron 35.0%
TGGAAAAATTCCTGGAAACT+CGG - Chr5:32965499-32965518 MsG0580026237.01.T01:CDS 35.0%
TTTGATTCTAGCAGCTATGA+TGG - Chr5:32965588-32965607 MsG0580026237.01.T01:intron 35.0%
! ACCTGTTTATTTAGGGTTAG+GGG - Chr5:32967642-32967661 MsG0580026237.01.T01:intron 35.0%
! ATCGTTCAGCTATTGCATTT+TGG + Chr5:32966847-32966866 None:intergenic 35.0%
! CTATTTGCAACTGTTTTCAG+GGG + Chr5:32966009-32966028 None:intergenic 35.0%
! CTTGGCCTATTGTTTTCAAA+AGG - Chr5:32968236-32968255 MsG0580026237.01.T01:intron 35.0%
! GATTTTCTATTGCAGCTCTT+CGG + Chr5:32968268-32968287 None:intergenic 35.0%
! GCTTATGGTAAGGGTATATT+TGG - Chr5:32965719-32965738 MsG0580026237.01.T01:CDS 35.0%
! GTTTCATTTTACCAGGTTGT+GGG + Chr5:32968623-32968642 None:intergenic 35.0%
! TAGGATTTTTCGTTGTAGAG+GGG + Chr5:32966327-32966346 None:intergenic 35.0%
! TCGTTCAGCTATTGCATTTT+GGG + Chr5:32966846-32966865 None:intergenic 35.0%
! TGTTTCATTTTACCAGGTTG+TGG + Chr5:32968624-32968643 None:intergenic 35.0%
!! TGGTGTAGATGTTTTGAACA+TGG - Chr5:32967803-32967822 MsG0580026237.01.T01:intron 35.0%
!!! GCACAATTGGTTTTCCTTTT+GGG + Chr5:32968094-32968113 None:intergenic 35.0%
!!! TGCACAATTGGTTTTCCTTT+TGG + Chr5:32968095-32968114 None:intergenic 35.0%
AAGCTAGTGAGATCCATTTG+AGG + Chr5:32966440-32966459 None:intergenic 40.0%
AAGTGTGTAGTGAACTGAAC+AGG - Chr5:32967049-32967068 MsG0580026237.01.T01:intron 40.0%
AATTGTGCACGATGAAAACC+CGG - Chr5:32968106-32968125 MsG0580026237.01.T01:intron 40.0%
ACATGATAGGTTCTCAACTC+AGG + Chr5:32967246-32967265 None:intergenic 40.0%
ACCCCTAACCCTAAATAAAC+AGG + Chr5:32967646-32967665 None:intergenic 40.0%
AGATATTAGAGATGGAGTGC+TGG - Chr5:32968334-32968353 MsG0580026237.01.T01:intron 40.0%
ATGGAGATTTCACACCTCAA+TGG - Chr5:32967876-32967895 MsG0580026237.01.T01:intron 40.0%
CACATGGATGGAAAAATTCC+TGG - Chr5:32965491-32965510 MsG0580026237.01.T01:CDS 40.0%
CACTACACACTTGCAACAAT+GGG + Chr5:32967041-32967060 None:intergenic 40.0%
CATTAGAGCTAGACTCAACT+CGG + Chr5:32967123-32967142 None:intergenic 40.0%
CTCCTTCACCTCATTAAAGA+TGG + Chr5:32966961-32966980 None:intergenic 40.0%
GAAATTCAACTCCCTTGTGA+TGG - Chr5:32969053-32969072 MsG0580026237.01.T01:CDS 40.0%
GCACTCTTGTACATCCAAAT+AGG - Chr5:32965939-32965958 MsG0580026237.01.T01:intron 40.0%
GTCTTGTATAGAGAGTGAGT+CGG - Chr5:32967330-32967349 MsG0580026237.01.T01:intron 40.0%
GTGAACTGAACAGGTTAATG+AGG - Chr5:32967058-32967077 MsG0580026237.01.T01:intron 40.0%
TAACACCCAAAACAGCCAAA+GGG - Chr5:32969104-32969123 MsG0580026237.01.T01:CDS 40.0%
TATCAAGTAGTGAGGATGGA+GGG + Chr5:32967159-32967178 None:intergenic 40.0%
TCACTACACACTTGCAACAA+TGG + Chr5:32967042-32967061 None:intergenic 40.0%
TCATCGACCAACATATTGCA+TGG - Chr5:32968650-32968669 MsG0580026237.01.T01:intron 40.0%
TCTTGTATAGAGAGTGAGTC+GGG - Chr5:32967331-32967350 MsG0580026237.01.T01:intron 40.0%
TGCTTTGGTAAATGCTAACC+AGG - Chr5:32968514-32968533 MsG0580026237.01.T01:intron 40.0%
TGTGTATGTACTCACCCAAA+AGG - Chr5:32968077-32968096 MsG0580026237.01.T01:intron 40.0%
TTAATCTGCGAGCTCGAAAA+CGG - Chr5:32969002-32969021 MsG0580026237.01.T01:CDS 40.0%
TTATCAAGTAGTGAGGATGG+AGG + Chr5:32967160-32967179 None:intergenic 40.0%
! AATCAAGTGTTGCAAAGGTG+AGG + Chr5:32966897-32966916 None:intergenic 40.0%
! ATTAAAGATGGGCTCACGTT+GGG + Chr5:32966949-32966968 None:intergenic 40.0%
! CTTGGTTATTTGTACCGATG+AGG + Chr5:32968825-32968844 None:intergenic 40.0%
! GTTTTACAACCATGACAGAG+AGG + Chr5:32966621-32966640 None:intergenic 40.0%
! TATCATTAGTCTAGAGCGAG+AGG + Chr5:32966473-32966492 None:intergenic 40.0%
! TGTGGAATCAAGTGTTGCAA+AGG + Chr5:32966902-32966921 None:intergenic 40.0%
! TTTGTTCCCTTTGGCTGTTT+TGG + Chr5:32969113-32969132 None:intergenic 40.0%
!! AAGTATTGTGACTTGCTGCA+AGG - Chr5:32968770-32968789 MsG0580026237.01.T01:intron 40.0%
!! AGTTGAGTCTAGCTCTAATG+CGG - Chr5:32967123-32967142 MsG0580026237.01.T01:intron 40.0%
!! GCTTTGAAGTTTCTCTCCAA+GGG + Chr5:32968056-32968075 None:intergenic 40.0%
!! TTGTTCCCTTTGGCTGTTTT+GGG + Chr5:32969112-32969131 None:intergenic 40.0%
!! TTTTGGGTGAGTACATACAC+AGG + Chr5:32968078-32968097 None:intergenic 40.0%
!!! CAGTTTTAGATGACAACCCT+TGG - Chr5:32968037-32968056 MsG0580026237.01.T01:intron 40.0%
!!! GTTTTTTCTCCTCTCTGTCA+TGG - Chr5:32966609-32966628 MsG0580026237.01.T01:intron 40.0%
AAGAACTCATCCGAGTTTCC+AGG + Chr5:32965512-32965531 None:intergenic 45.0%
ACGTACGCATGTCATAAGCA+CGG + Chr5:32965550-32965569 None:intergenic 45.0%
ACGTGAGCCCATCTTTAATG+AGG - Chr5:32966950-32966969 MsG0580026237.01.T01:intron 45.0%
AGCGAAGTTGAAAGAGAGCT+TGG - Chr5:32968218-32968237 MsG0580026237.01.T01:intron 45.0%
AGTCTAAACAAGCAAGGGGA+GGG - Chr5:32968288-32968307 MsG0580026237.01.T01:intron 45.0%
ATTCGGCTAGACAGTTGAAC+AGG - Chr5:32966737-32966756 MsG0580026237.01.T01:intron 45.0%
CAATTTGGCAATGTTGGTGC+AGG - Chr5:32968721-32968740 MsG0580026237.01.T01:intron 45.0%
CGATGGAAGGTGAAACTGAA+GGG + Chr5:32965274-32965293 None:intergenic 45.0%
GAACAGGCTGAAGCTAAAAG+TGG - Chr5:32966516-32966535 MsG0580026237.01.T01:intron 45.0%
GAGCTCGCAGATTAAGCTTA+AGG + Chr5:32968997-32969016 None:intergenic 45.0%
GCCCATCTTTAATGAGGTGA+AGG - Chr5:32966956-32966975 MsG0580026237.01.T01:intron 45.0%
GTAACACCCAAAACAGCCAA+AGG - Chr5:32969103-32969122 MsG0580026237.01.T01:CDS 45.0%
TCGCTTCTTTCCCATCACAA+GGG + Chr5:32969067-32969086 None:intergenic 45.0%
TGAGGTGTAGAGATGAGTCT+CGG + Chr5:32965365-32965384 None:intergenic 45.0%
TGCTTATGACATGCGTACGT+CGG - Chr5:32965550-32965569 MsG0580026237.01.T01:CDS 45.0%
! CATTAAAGATGGGCTCACGT+TGG + Chr5:32966950-32966969 None:intergenic 45.0%
! GGGTTTTCATCGTGCACAAT+TGG + Chr5:32968107-32968126 None:intergenic 45.0%
!! AAAGACATCGGCTCCTCAAA+TGG - Chr5:32966424-32966443 MsG0580026237.01.T01:CDS 45.0%
!! ATTGTGACTTGCTGCAAGGA+GGG - Chr5:32968774-32968793 MsG0580026237.01.T01:intron 45.0%
!! GGCTTTGAAGTTTCTCTCCA+AGG + Chr5:32968057-32968076 None:intergenic 45.0%
!! GTTGAGTCTAGCTCTAATGC+GGG - Chr5:32967124-32967143 MsG0580026237.01.T01:intron 45.0%
!! TATTGTGACTTGCTGCAAGG+AGG - Chr5:32968773-32968792 MsG0580026237.01.T01:intron 45.0%
!! TTGAGTCTAGCTCTAATGCG+GGG - Chr5:32967125-32967144 MsG0580026237.01.T01:intron 45.0%
!! TTGTGCGTTTTGGTCAAGAG+AGG - Chr5:32966188-32966207 MsG0580026237.01.T01:CDS 45.0%
AGACTCAACTCGGTAGAGCT+AGG + Chr5:32967113-32967132 None:intergenic 50.0%
AGATGACAGTGGAAACGAGG+AGG - Chr5:32965451-32965470 MsG0580026237.01.T01:CDS 50.0%
AGGAGATGACAGTGGAAACG+AGG - Chr5:32965448-32965467 MsG0580026237.01.T01:CDS 50.0%
AGGTGTAGAGATGAGTCTCG+GGG + Chr5:32965363-32965382 None:intergenic 50.0%
AGTTACTGAGCGGCATCAAG+AGG - Chr5:32966359-32966378 MsG0580026237.01.T01:CDS 50.0%
CAGTCTAAACAAGCAAGGGG+AGG - Chr5:32968287-32968306 MsG0580026237.01.T01:intron 50.0%
CCTTTCTCCTCCTCCTTGTT+CGG + Chr5:32965391-32965410 None:intergenic 50.0%
CGTACGCATGTCATAAGCAC+GGG + Chr5:32965549-32965568 None:intergenic 50.0%
CTACACCTCACTTCCGAACA+AGG - Chr5:32965375-32965394 MsG0580026237.01.T01:three_prime_UTR 50.0%
GAGGTGTAGAGATGAGTCTC+GGG + Chr5:32965364-32965383 None:intergenic 50.0%
GCGATGGAAGGTGAAACTGA+AGG + Chr5:32965275-32965294 None:intergenic 50.0%
GTCGCTTCTTTCCCATCACA+AGG + Chr5:32969068-32969087 None:intergenic 50.0%
TATAGAGAGTGAGTCGGGTC+AGG - Chr5:32967336-32967355 MsG0580026237.01.T01:intron 50.0%
TTACCAGGTTGTGGGCACTA+GGG + Chr5:32968615-32968634 None:intergenic 50.0%
TTTACCAGGTTGTGGGCACT+AGG + Chr5:32968616-32968635 None:intergenic 50.0%
! AGCTTAAGGCTTTCGGGTAC+AGG + Chr5:32968983-32969002 None:intergenic 50.0%
! AGGCTTTAGCTCATCTGAGC+AGG + Chr5:32967093-32967112 None:intergenic 50.0%
! GGCTTTAGCTCATCTGAGCA+GGG + Chr5:32967092-32967111 None:intergenic 50.0%
! TACCGATGAGGCGTCTATCT+CGG + Chr5:32968813-32968832 None:intergenic 50.0%
! TGCTGCGTGTTTGTGCGTTT+TGG - Chr5:32966178-32966197 MsG0580026237.01.T01:CDS 50.0%
! TTAAGGCTTTCGGGTACAGG+AGG + Chr5:32968980-32968999 None:intergenic 50.0%
!! GAAGTATCGTGCAACGTCGT+CGG - Chr5:32965410-32965429 MsG0580026237.01.T01:three_prime_UTR 50.0%
!! GAGAAAGCGCTTCACATGGA+TGG - Chr5:32965479-32965498 MsG0580026237.01.T01:CDS 50.0%
ACGCATGTCATAAGCACGGG+AGG + Chr5:32965546-32965565 None:intergenic 55.0%
AGTAGTGAGGATGGAGGGAC+TGG + Chr5:32967154-32967173 None:intergenic 55.0%
CACCTCACTTCCGAACAAGG+AGG - Chr5:32965378-32965397 MsG0580026237.01.T01:three_prime_UTR 55.0%
CAGTTCTATTCCGCCGAGGT+AGG - Chr5:32968951-32968970 MsG0580026237.01.T01:CDS 55.0%
CCGAACAAGGAGGAGGAGAA+AGG - Chr5:32965388-32965407 MsG0580026237.01.T01:three_prime_UTR 55.0%
CTCACTTCCGAACAAGGAGG+AGG - Chr5:32965381-32965400 MsG0580026237.01.T01:three_prime_UTR 55.0%
CTCCTCCTTGTTCGGAAGTG+AGG + Chr5:32965383-32965402 None:intergenic 55.0%
CTTGCAGTTCTATTCCGCCG+AGG - Chr5:32968947-32968966 MsG0580026237.01.T01:CDS 55.0%
GGTGTAGAGATGAGTCTCGG+GGG + Chr5:32965362-32965381 None:intergenic 55.0%
GTAGTGAGGATGGAGGGACT+GGG + Chr5:32967153-32967172 None:intergenic 55.0%
TGACAGTGGAAACGAGGAGG+AGG - Chr5:32965454-32965473 MsG0580026237.01.T01:CDS 55.0%
TGCCGAGATAGACGCCTCAT+CGG - Chr5:32968808-32968827 MsG0580026237.01.T01:intron 55.0%
! TTGCTGCAAGGAGGGTGTGA+GGG - Chr5:32968782-32968801 MsG0580026237.01.T01:intron 55.0%
!! GGTGGAGAAAGCGCTTCACA+TGG - Chr5:32965475-32965494 MsG0580026237.01.T01:CDS 55.0%
!! GTACAGGAGGTGTCCTACCT+CGG + Chr5:32968967-32968986 None:intergenic 55.0%
AGATGAGTCTCGGGGGCAGA+TGG + Chr5:32965355-32965374 None:intergenic 60.0%
CAGTGGAAACGAGGAGGAGG+TGG - Chr5:32965457-32965476 MsG0580026237.01.T01:CDS 60.0%
GAGTCGGGTCAGGTCTACTC+TGG - Chr5:32967346-32967365 MsG0580026237.01.T01:intron 60.0%
GGCAGAGGAGGAGATGACAG+TGG - Chr5:32965440-32965459 MsG0580026237.01.T01:CDS 60.0%
GTACCCTAGTGCCCACAACC+TGG - Chr5:32968609-32968628 MsG0580026237.01.T01:intron 60.0%
GTATCGTGCAACGTCGTCGG+CGG - Chr5:32965413-32965432 MsG0580026237.01.T01:three_prime_UTR 60.0%
! CTTGCTGCAAGGAGGGTGTG+AGG - Chr5:32968781-32968800 MsG0580026237.01.T01:intron 60.0%
! TGGCGACGGAAAGGGTTGGT+GGG + Chr5:32965305-32965324 None:intergenic 60.0%
!! GACGGAAAGGGTTGGTGGGA+GGG + Chr5:32965301-32965320 None:intergenic 60.0%
!! GATGGCGGGAGAAGAGAGAG+AGG + Chr5:32965337-32965356 None:intergenic 60.0%
GGAGAAGAGAGAGAGGCGGC+GGG + Chr5:32965330-32965349 None:intergenic 65.0%
GGCGGGAGAAGAGAGAGAGG+CGG + Chr5:32965334-32965353 None:intergenic 65.0%
GGGAGAAGAGAGAGAGGCGG+CGG + Chr5:32965331-32965350 None:intergenic 65.0%
GGTGTGGCGACGGAAAGGGT+TGG + Chr5:32965309-32965328 None:intergenic 65.0%
GTGGCGACGGAAAGGGTTGG+TGG + Chr5:32965306-32965325 None:intergenic 65.0%
GTGGGAGGGAGAGCGATGGA+AGG + Chr5:32965287-32965306 None:intergenic 65.0%
TGAGTCTCGGGGGCAGATGG+CGG + Chr5:32965352-32965371 None:intergenic 65.0%
! GTTGGTGGGAGGGAGAGCGA+TGG + Chr5:32965291-32965310 None:intergenic 65.0%
!! CAGGAGGTGTCCTACCTCGG+CGG + Chr5:32968964-32968983 None:intergenic 65.0%
!! CGACGGAAAGGGTTGGTGGG+AGG + Chr5:32965302-32965321 None:intergenic 65.0%
AGAGAGAGAGGCGGCGGGTG+TGG + Chr5:32965325-32965344 None:intergenic 70.0%
GAGTCTCGGGGGCAGATGGC+GGG + Chr5:32965351-32965370 None:intergenic 70.0%
GGCGGGTGTGGCGACGGAAA+GGG + Chr5:32965313-32965332 None:intergenic 70.0%
TCGTGCAACGTCGTCGGCGG+CGG - Chr5:32965416-32965435 MsG0580026237.01.T01:exon 70.0%
AGAGGCGGCGGGTGTGGCGA+CGG + Chr5:32965319-32965338 None:intergenic 75.0%
CGGCGGGTGTGGCGACGGAA+AGG + Chr5:32965314-32965333 None:intergenic 75.0%
TGCAACGTCGTCGGCGGCGG+CGG - Chr5:32965419-32965438 MsG0580026237.01.T01:exon 75.0%
! GTCGGCGGCGGCGGCAGAGG+AGG - Chr5:32965428-32965447 MsG0580026237.01.T01:exon 85.0%
! GTCGTCGGCGGCGGCGGCAG+AGG - Chr5:32965425-32965444 MsG0580026237.01.T01:exon 85.0%
Chromosome Type Strat End Strand Name
Chr5 gene 32965260 32969229 32965260 ID=MsG0580026237.01;Name=MsG0580026237.01
Chr5 mRNA 32965260 32969229 32965260 ID=MsG0580026237.01.T01;Parent=MsG0580026237.01;Name=MsG0580026237.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0.75|0.8|1|1|1|5|175|254
Chr5 exon 32965260 32965572 32965260 ID=MsG0580026237.01.T01:exon:13498;Parent=MsG0580026237.01.T01
Chr5 exon 32965660 32965746 32965660 ID=MsG0580026237.01.T01:exon:13497;Parent=MsG0580026237.01.T01
Chr5 exon 32966134 32966269 32966134 ID=MsG0580026237.01.T01:exon:13496;Parent=MsG0580026237.01.T01
Chr5 exon 32966350 32966449 32966350 ID=MsG0580026237.01.T01:exon:13495;Parent=MsG0580026237.01.T01
Chr5 exon 32968926 32969229 32968926 ID=MsG0580026237.01.T01:exon:13494;Parent=MsG0580026237.01.T01
Chr5 CDS 32968926 32969229 32968926 ID=MsG0580026237.01.T01:cds;Parent=MsG0580026237.01.T01
Chr5 CDS 32966350 32966449 32966350 ID=MsG0580026237.01.T01:cds;Parent=MsG0580026237.01.T01
Chr5 CDS 32966134 32966269 32966134 ID=MsG0580026237.01.T01:cds;Parent=MsG0580026237.01.T01
Chr5 CDS 32965660 32965746 32965660 ID=MsG0580026237.01.T01:cds;Parent=MsG0580026237.01.T01
Chr5 CDS 32965435 32965572 32965435 ID=MsG0580026237.01.T01:cds;Parent=MsG0580026237.01.T01
Chr5 three_prime_UTR 32965260 32965434 32965260 ID=MsG0580026237.01.T01:three_prime_utr;Parent=MsG0580026237.01.T01
Gene Sequence

>MsG0580026237.01.T01

ATGGATTCTCTCCCTTCAGTTTCACCTTCCATCGCTCTCCCTCCCACCAACCCTTTCCGTCGCCACACCCGCCGCCTCTCTCTCTTCTCCCGCCATCTGCCCCCGAGACTCATCTCTACACCTCACTTCCGAACAAGGAGGAGGAGAAAGGAAGTATCGTGCAACGTCGTCGGCGGCGGCGGCAGAGGAGGAGATGACAGTGGAAACGAGGAGGAGGTGGAGAAAGCGCTTCACATGGATGGAAAAATTCCTGGAAACTCGGATGAGTTCTTGAAGCAAGTTTCCTCCCGTGCTTATGACATGCTTTTAGATGACAACCCTTGGAGAGAAACTTCAAAGCCTGTGTATGTACTCACCCAAAAGGAAAACCAATTGTGCACGATGAAAACCCGGAGAAATATAAGCGAAGTTGAAAGAGAGCTTGGCCTATTGTTTTCAAAAGGAAGCAACCGAAGAGCTGCAATAGAAAATCAGTCTAAACAAGCAAGGGGAGGGACAAAATTTCCAATGCAAGTAGAAGATATTAGAGATGGAGTGCTGGTATTTGAAGATGAAAACGAGGCAGCTAAGTATTGTGACTTGCTGCAAGGAGGGTGTGAGGGTGTTGCCGAGATAGACGCCTCATCGATATTTGATCTTTGCAAGAAAATGAGGGCTCTTGCAGTTCTATTCCGCCGAGGTAGGACACCTCCTGTACCCGAAAGCCTTAAGCTTAATCTGCGAGCTCGAAAACGGTCCCTTGAAGATCAAGATGATCTGATATGA
Protein sequence

>MsG0580026237.01.T01

MDSLPSVSPSIALPPTNPFRRHTRRLSLFSRHLPPRLISTPHFRTRRRRKEVSCNVVGGGGRGGDDSGNEEEVEKALHMDGKIPGNSDEFLKQVSSRAYDMLLDDNPWRETSKPVYVLTQKENQLCTMKTRRNISEVERELGLLFSKGSNRRAAIENQSKQARGGTKFPMQVEDIRDGVLVFEDENEAAKYCDLLQGGCEGVAEIDASSIFDLCKKMRALAVLFRRGRTPPVPESLKLNLRARKRSLEDQDDLI*