Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021998.01.T01 | XP_003607621.1 | 95.062 | 243 | 8 | 1 | 1 | 243 | 1 | 239 | 1.05E-163 | 462 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000220.01 | MsG0480021998.01 | 0.804775 | 1.837151e-49 | 9.555404e-47 |
| MsG0180000475.01 | MsG0480021998.01 | 0.834932 | 2.251873e-56 | 2.712191e-53 |
| MsG0180000555.01 | MsG0480021998.01 | 0.838317 | 3.089429e-57 | 4.122373e-54 |
| MsG0180000585.01 | MsG0480021998.01 | 0.832887 | 7.313078e-56 | 8.284216e-53 |
| MsG0180000604.01 | MsG0480021998.01 | 0.847474 | 1.130030e-59 | 2.010925e-56 |
| MsG0180000680.01 | MsG0480021998.01 | 0.833617 | 4.811107e-56 | 5.570146e-53 |
| MsG0180000702.01 | MsG0480021998.01 | 0.817129 | 3.870837e-52 | 2.795110e-49 |
| MsG0180000703.01 | MsG0480021998.01 | 0.831431 | 1.675718e-55 | 1.818012e-52 |
| MsG0180000741.01 | MsG0480021998.01 | 0.840225 | 9.883591e-58 | 1.398660e-54 |
| MsG0180000797.01 | MsG0480021998.01 | 0.810612 | 1.057047e-50 | 6.401403e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021998.01.T01 | MTR_4g080340 | 95.062 | 243 | 8 | 1 | 1 | 243 | 1 | 239 | 1.28e-167 | 462 |
| MsG0480021998.01.T01 | MTR_2g083910 | 53.257 | 261 | 76 | 10 | 1 | 243 | 1 | 233 | 5.09e-72 | 220 |
| MsG0480021998.01.T01 | MTR_2g083910 | 53.282 | 259 | 75 | 10 | 1 | 241 | 1 | 231 | 1.38e-70 | 217 |
| MsG0480021998.01.T01 | MTR_8g086820 | 45.020 | 251 | 121 | 7 | 1 | 243 | 1 | 242 | 1.17e-50 | 166 |
| MsG0480021998.01.T01 | MTR_2g016760 | 41.129 | 248 | 118 | 6 | 1 | 240 | 1 | 228 | 3.67e-45 | 155 |
| MsG0480021998.01.T01 | MTR_2g078620 | 45.614 | 114 | 44 | 5 | 128 | 241 | 225 | 320 | 1.07e-16 | 78.2 |
Find 44 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCAAAGTTATAGAAGA+AGG | 0.481928 | 4:+66946291 | MsG0480021998.01.T01:CDS |
| AAAGTTATAGAAGAAGGAGA+AGG | 0.599626 | 4:+66946297 | MsG0480021998.01.T01:CDS |
| AAATTGGTTTCATTGATACC+TGG | 0.540169 | 4:+66946410 | MsG0480021998.01.T01:CDS |
| AATTGGAGAGTACCAGATAA+GGG | 0.506303 | 4:+66946587 | MsG0480021998.01.T01:CDS |
| AATTGTTGTTTCAAACCAAA+AGG | 0.459274 | 4:-66945979 | None:intergenic |
| AGAATTCGGAGAATTCTCAA+AGG | 0.627578 | 4:+66946211 | MsG0480021998.01.T01:CDS |
| AGCCTTATTTATCTGAGGCT+TGG | 0.418495 | 4:+66946525 | MsG0480021998.01.T01:CDS |
| AGTAAGAGATTTGTGTCTTG+AGG | 0.545501 | 4:-66946053 | None:intergenic |
| ATACCTGGTTTACAAAGACT+TGG | 0.496252 | 4:+66946425 | MsG0480021998.01.T01:CDS |
| ATCCCTTCTGAACGAGAATT+CGG | 0.393301 | 4:+66946197 | MsG0480021998.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCCAAAGTTATAGAAGA+AGG | + | Chr4:66946291-66946310 | MsG0480021998.01.T01:CDS | 30.0% | |
| AAAGTTATAGAAGAAGGAGA+AGG | + | Chr4:66946297-66946316 | MsG0480021998.01.T01:CDS | 30.0% | |
| CTAAAAGGGTTTATGGATTT+GGG | + | Chr4:66946356-66946375 | MsG0480021998.01.T01:CDS | 30.0% | |
| CTTGAGTTTAAAGAGCTAAA+AGG | + | Chr4:66946341-66946360 | MsG0480021998.01.T01:CDS | 30.0% | |
| GAAAATTATGACGTAGAAGA+AGG | + | Chr4:66946452-66946471 | MsG0480021998.01.T01:CDS | 30.0% | |
| GGAAGACAAAGATTCAAAAT+TGG | + | Chr4:66946394-66946413 | MsG0480021998.01.T01:CDS | 30.0% | |
| TAAAGAGCTAAAAGGGTTTA+TGG | + | Chr4:66946349-66946368 | MsG0480021998.01.T01:CDS | 30.0% | |
| TAGTGAAAATCATCATCAGA+AGG | + | Chr4:66946241-66946260 | MsG0480021998.01.T01:CDS | 30.0% | |
| TTCTCCTTCTTCTATAACTT+TGG | - | Chr4:66946298-66946317 | None:intergenic | 30.0% | |
| AGAATTCGGAGAATTCTCAA+AGG | + | Chr4:66946211-66946230 | MsG0480021998.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 66945945 | 66946676 | 66945945 | ID=MsG0480021998.01;Name=MsG0480021998.01 |
| Chr4 | mRNA | 66945945 | 66946676 | 66945945 | ID=MsG0480021998.01.T01;Parent=MsG0480021998.01;Name=MsG0480021998.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|243 |
| Chr4 | exon | 66945945 | 66946676 | 66945945 | ID=MsG0480021998.01.T01:exon:20937;Parent=MsG0480021998.01.T01 |
| Chr4 | CDS | 66945945 | 66946676 | 66945945 | ID=MsG0480021998.01.T01:cds;Parent=MsG0480021998.01.T01 |
| Gene Sequence |
>MsG0480021998.01.T01 ATGTCTGCAGAACAAATTCTAACTTTGTTAGATTCCTTTTGGTTTGAAACAACAATTCTCACCAACAAAAATCCTTCAAACTTTCACACAAAAGTTGAAGAAATTCTTCCTCAAGACACAAATCTCTTACTTGTTCCAACTCCAAACCTTGATGTTAGGTTCTATAGTGAACAAAACTTGAGCATCATAGGATCAGTTTTCTCTGATTCTCCATCACCAAATTCAGTCCTCACCTCATCAAAGCTAAGAACAATCCCTTCTGAACGAGAATTCGGAGAATTCTCAAAGGAGACTAGTAGTGAAAATCATCATCAGAAGGAAGATGTTGCATACACTAAGAAAAAACAAAGCCAAAGTTATAGAAGAAGGAGAAGGAAAAGTAGGAGTTTATCAGAACTTGAGTTTAAAGAGCTAAAAGGGTTTATGGATTTGGGTTTTGTGTTTTCTGAGGAAGACAAAGATTCAAAATTGGTTTCATTGATACCTGGTTTACAAAGACTTGGAAAAGAAAATTATGACGTAGAAGAAGGTGAAGATGAAGAACATAAGAAGATTGATGAACATGTTTTATCTGATCATAAGCCTTATTTATCTGAGGCTTGGGATGTTCTTGATCAAAGGGAGAGAAAAAATCCATTAGTGAATTGGAGAGTACCAGATAAGGGTAGTGAGATTGACATGAAAGACAATCTTAAATTCTGGGCTCATGCAGTTGCATCCATTGCTAGATAA |
| Protein sequence |
>MsG0480021998.01.T01 MSAEQILTLLDSFWFETTILTNKNPSNFHTKVEEILPQDTNLLLVPTPNLDVRFYSEQNLSIIGSVFSDSPSPNSVLTSSKLRTIPSEREFGEFSKETSSENHHQKEDVAYTKKKQSQSYRRRRRKSRSLSELEFKELKGFMDLGFVFSEEDKDSKLVSLIPGLQRLGKENYDVEEGEDEEHKKIDEHVLSDHKPYLSEAWDVLDQRERKNPLVNWRVPDKGSEIDMKDNLKFWAHAVASIAR* |
