AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021998.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021998.01.T01 MTR_4g080340 95.062 243 8 1 1 243 1 239 1.28e-167 462
MsG0480021998.01.T01 MTR_2g083910 53.257 261 76 10 1 243 1 233 5.09e-72 220
MsG0480021998.01.T01 MTR_2g083910 53.282 259 75 10 1 241 1 231 1.38e-70 217
MsG0480021998.01.T01 MTR_8g086820 45.020 251 121 7 1 243 1 242 1.17e-50 166
MsG0480021998.01.T01 MTR_2g016760 41.129 248 118 6 1 240 1 228 3.67e-45 155
MsG0480021998.01.T01 MTR_2g078620 45.614 114 44 5 128 241 225 320 1.07e-16 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021998.01.T01 AT2G42760 46.961 181 83 5 73 243 89 266 2.01e-33 122

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAGCTAAAAGGGTTTA+TGG 0.170053 4:+66946349 MsG0480021998.01.T01:CDS
GCTAAAAGGGTTTATGGATT+TGG 0.205025 4:+66946355 MsG0480021998.01.T01:CDS
CTTGAGTTTAAAGAGCTAAA+AGG 0.220257 4:+66946341 MsG0480021998.01.T01:CDS
CTAAAAGGGTTTATGGATTT+GGG 0.271870 4:+66946356 MsG0480021998.01.T01:CDS
TAACTTTGTTAGATTCCTTT+TGG 0.273536 4:+66945964 MsG0480021998.01.T01:CDS
TTCTCCTTCTTCTATAACTT+TGG 0.283198 4:-66946295 None:intergenic
GGAAGACAAAGATTCAAAAT+TGG 0.293253 4:+66946394 MsG0480021998.01.T01:CDS
GATGAGGTGAGGACTGAATT+TGG 0.313702 4:-66946161 None:intergenic
TGAAAGACAATCTTAAATTC+TGG 0.319158 4:+66946624 MsG0480021998.01.T01:CDS
CAACTCCAAACCTTGATGTT+AGG 0.320056 4:+66946081 MsG0480021998.01.T01:CDS
TCAATCTCACTACCCTTATC+TGG 0.351221 4:-66946599 None:intergenic
TCCCAAGCCTCAGATAAATA+AGG 0.366051 4:-66946527 None:intergenic
GCCTTATTTATCTGAGGCTT+GGG 0.368719 4:+66946526 MsG0480021998.01.T01:CDS
GAACAAAACTTGAGCATCAT+AGG 0.370015 4:+66946113 MsG0480021998.01.T01:CDS
ATCCCTTCTGAACGAGAATT+CGG 0.393301 4:+66946197 MsG0480021998.01.T01:CDS
GGTACTCTCCAATTCACTAA+TGG 0.398825 4:-66946578 None:intergenic
GAAAGACAATCTTAAATTCT+GGG 0.415251 4:+66946625 MsG0480021998.01.T01:CDS
AGCCTTATTTATCTGAGGCT+TGG 0.418495 4:+66946525 MsG0480021998.01.T01:CDS
TTGAGTTTAAAGAGCTAAAA+GGG 0.432583 4:+66946342 MsG0480021998.01.T01:CDS
AATTGTTGTTTCAAACCAAA+AGG 0.459274 4:-66945979 None:intergenic
GAAGAAGGAGAAGGAAAAGT+AGG 0.462102 4:+66946306 MsG0480021998.01.T01:CDS
AAAGCCAAAGTTATAGAAGA+AGG 0.481928 4:+66946291 MsG0480021998.01.T01:CDS
TTGGGATGTTCTTGATCAAA+GGG 0.484785 4:+66946544 MsG0480021998.01.T01:CDS
GAAAATTATGACGTAGAAGA+AGG 0.488373 4:+66946452 MsG0480021998.01.T01:CDS
TAGAACCTAACATCAAGGTT+TGG 0.495307 4:-66946086 None:intergenic
ATACCTGGTTTACAAAGACT+TGG 0.496252 4:+66946425 MsG0480021998.01.T01:CDS
AATTGGAGAGTACCAGATAA+GGG 0.506303 4:+66946587 MsG0480021998.01.T01:CDS
CTTGGGATGTTCTTGATCAA+AGG 0.518140 4:+66946543 MsG0480021998.01.T01:CDS
TTCTTAGCTTTGATGAGGTG+AGG 0.519531 4:-66946172 None:intergenic
GAATTGGAGAGTACCAGATA+AGG 0.523292 4:+66946586 MsG0480021998.01.T01:CDS
TCTCCGAATTCTCGTTCAGA+AGG 0.528702 4:-66946200 None:intergenic
CACTATAGAACCTAACATCA+AGG 0.532184 4:-66946091 None:intergenic
TTTCCAAGTCTTTGTAAACC+AGG 0.538680 4:-66946428 None:intergenic
AAATTGGTTTCATTGATACC+TGG 0.540169 4:+66946410 MsG0480021998.01.T01:CDS
AGTAAGAGATTTGTGTCTTG+AGG 0.545501 4:-66946053 None:intergenic
CTCCGAATTCTCGTTCAGAA+GGG 0.549233 4:-66946199 None:intergenic
CTAACATCAAGGTTTGGAGT+TGG 0.554839 4:-66946080 None:intergenic
TGGTCATTGTTATCTAGCAA+TGG 0.557526 4:-66946663 None:intergenic
TCATAAGCCTTATTTATCTG+AGG 0.578723 4:+66946520 MsG0480021998.01.T01:CDS
GATTGTTCTTAGCTTTGATG+AGG 0.591944 4:-66946177 None:intergenic
AAAGTTATAGAAGAAGGAGA+AGG 0.599626 4:+66946297 MsG0480021998.01.T01:CDS
TAGTGAAAATCATCATCAGA+AGG 0.610824 4:+66946241 MsG0480021998.01.T01:CDS
GTGAGGACTGAATTTGGTGA+TGG 0.623869 4:-66946155 None:intergenic
AGAATTCGGAGAATTCTCAA+AGG 0.627578 4:+66946211 MsG0480021998.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAAAAATCCATTAGTGAAT+TGG + Chr4:66946570-66946589 MsG0480021998.01.T01:CDS 25.0%
! GAAAGACAATCTTAAATTCT+GGG + Chr4:66946625-66946644 MsG0480021998.01.T01:CDS 25.0%
! TGAAAGACAATCTTAAATTC+TGG + Chr4:66946624-66946643 MsG0480021998.01.T01:CDS 25.0%
! TTGAGTTTAAAGAGCTAAAA+GGG + Chr4:66946342-66946361 MsG0480021998.01.T01:CDS 25.0%
!! AAAGTTTGAAGGATTTTTGT+TGG - Chr4:66946009-66946028 None:intergenic 25.0%
!! TAACTTTGTTAGATTCCTTT+TGG + Chr4:66945964-66945983 MsG0480021998.01.T01:CDS 25.0%
!!! AATTGTTGTTTCAAACCAAA+AGG - Chr4:66945982-66946001 None:intergenic 25.0%
AAAGCCAAAGTTATAGAAGA+AGG + Chr4:66946291-66946310 MsG0480021998.01.T01:CDS 30.0%
AAAGTTATAGAAGAAGGAGA+AGG + Chr4:66946297-66946316 MsG0480021998.01.T01:CDS 30.0%
CTAAAAGGGTTTATGGATTT+GGG + Chr4:66946356-66946375 MsG0480021998.01.T01:CDS 30.0%
CTTGAGTTTAAAGAGCTAAA+AGG + Chr4:66946341-66946360 MsG0480021998.01.T01:CDS 30.0%
GAAAATTATGACGTAGAAGA+AGG + Chr4:66946452-66946471 MsG0480021998.01.T01:CDS 30.0%
GGAAGACAAAGATTCAAAAT+TGG + Chr4:66946394-66946413 MsG0480021998.01.T01:CDS 30.0%
TAAAGAGCTAAAAGGGTTTA+TGG + Chr4:66946349-66946368 MsG0480021998.01.T01:CDS 30.0%
TAGTGAAAATCATCATCAGA+AGG + Chr4:66946241-66946260 MsG0480021998.01.T01:CDS 30.0%
TTCTCCTTCTTCTATAACTT+TGG - Chr4:66946298-66946317 None:intergenic 30.0%
! AAATTGGTTTCATTGATACC+TGG + Chr4:66946410-66946429 MsG0480021998.01.T01:CDS 30.0%
! ACTTTTGTGTGAAAGTTTGA+AGG - Chr4:66946020-66946039 None:intergenic 30.0%
!! TCATAAGCCTTATTTATCTG+AGG + Chr4:66946520-66946539 MsG0480021998.01.T01:CDS 30.0%
AGAATTCGGAGAATTCTCAA+AGG + Chr4:66946211-66946230 MsG0480021998.01.T01:CDS 35.0%
AGTAAGAGATTTGTGTCTTG+AGG - Chr4:66946056-66946075 None:intergenic 35.0%
ATACCTGGTTTACAAAGACT+TGG + Chr4:66946425-66946444 MsG0480021998.01.T01:CDS 35.0%
CACTATAGAACCTAACATCA+AGG - Chr4:66946094-66946113 None:intergenic 35.0%
GAACAAAACTTGAGCATCAT+AGG + Chr4:66946113-66946132 MsG0480021998.01.T01:CDS 35.0%
GCTAAAAGGGTTTATGGATT+TGG + Chr4:66946355-66946374 MsG0480021998.01.T01:CDS 35.0%
TAGAACCTAACATCAAGGTT+TGG - Chr4:66946089-66946108 None:intergenic 35.0%
TTGGGATGTTCTTGATCAAA+GGG + Chr4:66946544-66946563 MsG0480021998.01.T01:CDS 35.0%
TTTCCAAGTCTTTGTAAACC+AGG - Chr4:66946431-66946450 None:intergenic 35.0%
!! AATTGGAGAGTACCAGATAA+GGG + Chr4:66946587-66946606 MsG0480021998.01.T01:CDS 35.0%
!! GATTGTTCTTAGCTTTGATG+AGG - Chr4:66946180-66946199 None:intergenic 35.0%
!! TTTGGGTTTTGTGTTTTCTG+AGG + Chr4:66946373-66946392 MsG0480021998.01.T01:CDS 35.0%
ATCCCTTCTGAACGAGAATT+CGG + Chr4:66946197-66946216 MsG0480021998.01.T01:CDS 40.0%
CAACTCCAAACCTTGATGTT+AGG + Chr4:66946081-66946100 MsG0480021998.01.T01:CDS 40.0%
CTTGGGATGTTCTTGATCAA+AGG + Chr4:66946543-66946562 MsG0480021998.01.T01:CDS 40.0%
GAAGAAGGAGAAGGAAAAGT+AGG + Chr4:66946306-66946325 MsG0480021998.01.T01:CDS 40.0%
GGTACTCTCCAATTCACTAA+TGG - Chr4:66946581-66946600 None:intergenic 40.0%
TCAATCTCACTACCCTTATC+TGG - Chr4:66946602-66946621 None:intergenic 40.0%
TCCCAAGCCTCAGATAAATA+AGG - Chr4:66946530-66946549 None:intergenic 40.0%
! CTAACATCAAGGTTTGGAGT+TGG - Chr4:66946083-66946102 None:intergenic 40.0%
!! AGCCTTATTTATCTGAGGCT+TGG + Chr4:66946525-66946544 MsG0480021998.01.T01:CDS 40.0%
!! GAATTGGAGAGTACCAGATA+AGG + Chr4:66946586-66946605 MsG0480021998.01.T01:CDS 40.0%
!! GCCTTATTTATCTGAGGCTT+GGG + Chr4:66946526-66946545 MsG0480021998.01.T01:CDS 40.0%
!! TTCTTAGCTTTGATGAGGTG+AGG - Chr4:66946175-66946194 None:intergenic 40.0%
CTCCGAATTCTCGTTCAGAA+GGG - Chr4:66946202-66946221 None:intergenic 45.0%
GATGAGGTGAGGACTGAATT+TGG - Chr4:66946164-66946183 None:intergenic 45.0%
TCTCCGAATTCTCGTTCAGA+AGG - Chr4:66946203-66946222 None:intergenic 45.0%
! GTGAGGACTGAATTTGGTGA+TGG - Chr4:66946158-66946177 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66945945 66946676 66945945 ID=MsG0480021998.01;Name=MsG0480021998.01
Chr4 mRNA 66945945 66946676 66945945 ID=MsG0480021998.01.T01;Parent=MsG0480021998.01;Name=MsG0480021998.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|243
Chr4 exon 66945945 66946676 66945945 ID=MsG0480021998.01.T01:exon:20937;Parent=MsG0480021998.01.T01
Chr4 CDS 66945945 66946676 66945945 ID=MsG0480021998.01.T01:cds;Parent=MsG0480021998.01.T01
Gene Sequence

>MsG0480021998.01.T01

ATGTCTGCAGAACAAATTCTAACTTTGTTAGATTCCTTTTGGTTTGAAACAACAATTCTCACCAACAAAAATCCTTCAAACTTTCACACAAAAGTTGAAGAAATTCTTCCTCAAGACACAAATCTCTTACTTGTTCCAACTCCAAACCTTGATGTTAGGTTCTATAGTGAACAAAACTTGAGCATCATAGGATCAGTTTTCTCTGATTCTCCATCACCAAATTCAGTCCTCACCTCATCAAAGCTAAGAACAATCCCTTCTGAACGAGAATTCGGAGAATTCTCAAAGGAGACTAGTAGTGAAAATCATCATCAGAAGGAAGATGTTGCATACACTAAGAAAAAACAAAGCCAAAGTTATAGAAGAAGGAGAAGGAAAAGTAGGAGTTTATCAGAACTTGAGTTTAAAGAGCTAAAAGGGTTTATGGATTTGGGTTTTGTGTTTTCTGAGGAAGACAAAGATTCAAAATTGGTTTCATTGATACCTGGTTTACAAAGACTTGGAAAAGAAAATTATGACGTAGAAGAAGGTGAAGATGAAGAACATAAGAAGATTGATGAACATGTTTTATCTGATCATAAGCCTTATTTATCTGAGGCTTGGGATGTTCTTGATCAAAGGGAGAGAAAAAATCCATTAGTGAATTGGAGAGTACCAGATAAGGGTAGTGAGATTGACATGAAAGACAATCTTAAATTCTGGGCTCATGCAGTTGCATCCATTGCTAGATAA

Protein sequence

>MsG0480021998.01.T01

MSAEQILTLLDSFWFETTILTNKNPSNFHTKVEEILPQDTNLLLVPTPNLDVRFYSEQNLSIIGSVFSDSPSPNSVLTSSKLRTIPSEREFGEFSKETSSENHHQKEDVAYTKKKQSQSYRRRRRKSRSLSELEFKELKGFMDLGFVFSEEDKDSKLVSLIPGLQRLGKENYDVEEGEDEEHKKIDEHVLSDHKPYLSEAWDVLDQRERKNPLVNWRVPDKGSEIDMKDNLKFWAHAVASIAR*