AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031618.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031618.01.T01 MTR_7g086720 68.103 232 70 2 1 231 1 229 1.03e-105 311
MsG0680031618.01.T01 MTR_6g027550 96.528 144 5 0 88 231 1 144 2.34e-102 300
MsG0680031618.01.T01 MTR_4g114140 57.692 182 76 1 57 237 76 257 1.25e-73 229
MsG0680031618.01.T01 MTR_2g085300 56.284 183 79 1 56 237 83 265 7.94e-70 220
MsG0680031618.01.T01 MTR_6g011080 47.107 242 112 5 8 237 32 269 1.05e-69 220
MsG0680031618.01.T01 MTR_0072s0020 51.500 200 96 1 39 237 72 271 5.25e-69 218
MsG0680031618.01.T01 MTR_4g022240 51.412 177 85 1 56 231 73 249 1.11e-64 207
MsG0680031618.01.T01 MTR_1g046740 50.538 186 91 1 53 237 93 278 5.09e-59 192
MsG0680031618.01.T01 MTR_0662s0030 56.250 144 62 1 95 237 15 158 1.38e-56 182
MsG0680031618.01.T01 MTR_1g108280 46.961 181 95 1 52 231 46 226 4.43e-54 179
MsG0680031618.01.T01 MTR_4g008010 35.965 228 139 3 11 231 10 237 7.76e-41 144
MsG0680031618.01.T01 MTR_4g077600 43.860 57 32 0 168 224 5 61 2.55e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031618.01.T01 AT3G03690 61.429 210 77 2 22 231 24 229 2.24e-88 266
MsG0680031618.01.T01 AT4G27480 57.923 183 76 1 56 237 80 262 6.21e-73 228
MsG0680031618.01.T01 AT4G27480 57.923 183 76 1 56 237 80 262 6.21e-73 228
MsG0680031618.01.T01 AT4G27480 57.923 183 76 1 56 237 80 262 6.21e-73 228
MsG0680031618.01.T01 AT3G15350 56.284 183 79 1 56 237 81 263 3.57e-72 226
MsG0680031618.01.T01 AT3G15350 56.284 183 79 1 56 237 81 263 3.57e-72 226
MsG0680031618.01.T01 AT3G03690 59.064 171 66 2 22 192 24 190 4.23e-69 211
MsG0680031618.01.T01 AT1G53100 47.458 236 111 4 8 231 25 259 6.28e-69 218
MsG0680031618.01.T01 AT1G53100 47.458 236 111 4 8 231 25 259 7.29e-69 218
MsG0680031618.01.T01 AT5G15050 55.738 183 80 1 56 237 88 270 2.09e-68 217
MsG0680031618.01.T01 AT5G39990 54.301 186 84 1 53 237 98 283 8.70e-67 213
MsG0680031618.01.T01 AT1G03520 49.000 200 97 2 39 237 1 196 1.33e-64 205
MsG0680031618.01.T01 AT1G03520 48.515 202 99 2 37 237 88 285 7.85e-64 205
MsG0680031618.01.T01 AT4G03340 48.000 200 102 2 39 237 88 286 4.86e-63 203
MsG0680031618.01.T01 AT2G37585 51.412 177 83 2 56 231 59 233 4.82e-62 199
MsG0680031618.01.T01 AT3G24040 45.699 186 100 1 53 237 66 251 5.86e-54 179
MsG0680031618.01.T01 AT1G71070 43.646 181 101 1 52 231 45 225 1.30e-49 167
MsG0680031618.01.T01 AT4G03340 41.500 200 92 3 39 237 88 263 2.25e-47 162

Find 48 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATTAAGTTTGGGAATA+TGG 0.178104 6:+23399052 None:intergenic
CTTCACAATCATCTCTAAAA+TGG 0.202291 6:-23399031 MsG0680031618.01.T01:exon
TTCTTCTTAGGAGTTGTAAA+TGG 0.207307 6:-23398588 MsG0680031618.01.T01:CDS
TAAGGCTGGTATGGTTAATT+TGG 0.213797 6:+23398942 None:intergenic
ATTAAATATGCAAAACTAAT+TGG 0.222896 6:+23398856 None:intergenic
GCCAAATATCTGATTTCTTT+TGG 0.224723 6:+23397801 None:intergenic
GCCAAAAGAAATCAGATATT+TGG 0.232766 6:-23397802 MsG0680031618.01.T01:CDS
ATGCTATGGCAATTCTTCTT+AGG 0.239237 6:-23398600 MsG0680031618.01.T01:CDS
GATTGTGAAGAAATTAAGTT+TGG 0.263708 6:+23399044 None:intergenic
ACATCTCTATGATCTTTCTT+TGG 0.304538 6:+23398736 None:intergenic
AAGGTTGTGTTCATTGGTTT+TGG 0.309897 6:+23398907 None:intergenic
CATTCACCATAGCAGTCATT+TGG 0.315591 6:-23398340 MsG0680031618.01.T01:CDS
AGTAACTTTGTTACATATAA+AGG 0.317512 6:-23398649 MsG0680031618.01.T01:CDS
AAATTATTATTTGATTCATA+TGG 0.318689 6:-23398770 MsG0680031618.01.T01:CDS
GAAATTAAGTTTGGGAATAT+GGG 0.344225 6:+23399053 None:intergenic
ATTCACCATAGCAGTCATTT+GGG 0.401509 6:-23398339 MsG0680031618.01.T01:CDS
TGAATCAAATAATAATTTCC+TGG 0.403261 6:+23398775 None:intergenic
TTGGTCAAGTAGGAAATGTT+TGG 0.403827 6:-23398684 MsG0680031618.01.T01:CDS
TAGTGCATCTGATTATCCTT+TGG 0.430100 6:-23398548 MsG0680031618.01.T01:CDS
ATGTTGAAGGTTGTGTTCAT+TGG 0.435000 6:+23398901 None:intergenic
TGAATGAAATTCATATGCTT+TGG 0.436558 6:+23398357 None:intergenic
TATTTGATTCATATGGATCA+TGG 0.436582 6:-23398763 MsG0680031618.01.T01:CDS
GAAAATCAAAAGGTTGTTGA+AGG 0.437111 6:-23398809 MsG0680031618.01.T01:CDS
ACCATAGCAGTCATTTGGGT+TGG 0.447311 6:-23398335 MsG0680031618.01.T01:CDS
TTAGGAGTTGTAAATGGGAT+TGG 0.455353 6:-23398582 MsG0680031618.01.T01:CDS
TATCCTTTGGTTACACAAGA+TGG 0.455593 6:-23398535 MsG0680031618.01.T01:intron
GGCTGGTATGGTTAATTTGG+TGG 0.469378 6:+23398945 None:intergenic
TATTTAATTTCAGGTTCAAA+AGG 0.470731 6:-23398841 MsG0680031618.01.T01:CDS
GTTGGTATTGGAGATGTTGA+AGG 0.471056 6:+23398888 None:intergenic
TCTTCTTAGGAGTTGTAAAT+GGG 0.473649 6:-23398587 MsG0680031618.01.T01:CDS
ATTGTGAAGAAATTAAGTTT+GGG 0.473998 6:+23399045 None:intergenic
TTGAAGGCTTTGTACCATCC+AGG 0.476164 6:-23398793 MsG0680031618.01.T01:CDS
TATATAGTTTAAAAGCTGTT+GGG 0.491319 6:+23397756 None:intergenic
TTCTACAACTCTTCATGCTA+TGG 0.494826 6:-23398614 MsG0680031618.01.T01:CDS
CAATGTTGAGGTAAGGATAG+AGG 0.500511 6:+23398987 None:intergenic
GTATATAGTTTAAAAGCTGT+TGG 0.503019 6:+23397755 None:intergenic
ATGCTTTGGAATTCCAGAAA+AGG 0.506923 6:+23398371 None:intergenic
TCAAATAATAATTTCCTGGA+TGG 0.514443 6:+23398779 None:intergenic
AGAGTTGTAGAAAGCATAGT+TGG 0.528565 6:+23398625 None:intergenic
GCTGGTATGGTTAATTTGGT+GGG 0.529232 6:+23398946 None:intergenic
GAGATGCTATGAAAATCAAA+AGG 0.539134 6:-23398819 MsG0680031618.01.T01:CDS
CAAAGAACAATGTTGAGGTA+AGG 0.540779 6:+23398980 None:intergenic
TTGGAATTCCAGAAAAGGCA+TGG 0.553652 6:+23398376 None:intergenic
AAAGAAATCAGATATTTGGC+AGG 0.557557 6:-23397798 MsG0680031618.01.T01:CDS
TCCAACCCAAATGACTGCTA+TGG 0.598350 6:+23398334 None:intergenic
AAGAACAAAGAACAATGTTG+AGG 0.619282 6:+23398975 None:intergenic
TTTGGCAGGTAACTAAGCAA+AGG 0.625832 6:-23397784 MsG0680031618.01.T01:CDS
ATACCATCTTGTGTAACCAA+AGG 0.660551 6:+23398532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGATTCAAAAAATAAAA+TGG + Chr6:23398314-23398333 None:intergenic 10.0%
!! AAATTATTATTTGATTCATA+TGG - Chr6:23398022-23398041 MsG0680031618.01.T01:intron 10.0%
!!! AATTAATAAACTTTTAGTAT+AGG + Chr6:23398821-23398840 None:intergenic 10.0%
!!! ATTAATAAACTTTTAGTATA+GGG + Chr6:23398820-23398839 None:intergenic 10.0%
!!! ATTAGAATTTTTAAGTAAAA+TGG + Chr6:23398906-23398925 None:intergenic 10.0%
!! ATTAAATATGCAAAACTAAT+TGG + Chr6:23397939-23397958 None:intergenic 15.0%
!! TTATAAAATATTTAGCCTAT+TGG - Chr6:23398645-23398664 MsG0680031618.01.T01:CDS 15.0%
!!! TTAATAAACTTTTAGTATAG+GGG + Chr6:23398819-23398838 None:intergenic 15.0%
!! AGTAACTTTGTTACATATAA+AGG - Chr6:23398143-23398162 MsG0680031618.01.T01:intron 20.0%
!! ATTGTGAAGAAATTAAGTTT+GGG + Chr6:23397750-23397769 None:intergenic 20.0%
!! TATATAGTTTAAAAGCTGTT+GGG + Chr6:23399039-23399058 None:intergenic 20.0%
!! TGAATCAAATAATAATTTCC+TGG + Chr6:23398020-23398039 None:intergenic 20.0%
!!! ACTAATTGGATATTTTTTGT+TGG + Chr6:23397925-23397944 None:intergenic 20.0%
!!! AGTTTTGCATATTTAATTTC+AGG - Chr6:23397942-23397961 MsG0680031618.01.T01:intron 20.0%
!!! TATTTAATTTCAGGTTCAAA+AGG - Chr6:23397951-23397970 MsG0680031618.01.T01:intron 20.0%
! AGAAATTAAGTTTGGGAATA+TGG + Chr6:23397743-23397762 None:intergenic 25.0%
! CCAATTTAGTCACTAAAATT+TGG + Chr6:23398861-23398880 None:intergenic 25.0%
! GAAATTAAGTTTGGGAATAT+GGG + Chr6:23397742-23397761 None:intergenic 25.0%
! GATTGTGAAGAAATTAAGTT+TGG + Chr6:23397751-23397770 None:intergenic 25.0%
! GTATATAGTTTAAAAGCTGT+TGG + Chr6:23399040-23399059 None:intergenic 25.0%
! TATTTGATTCATATGGATCA+TGG - Chr6:23398029-23398048 MsG0680031618.01.T01:intron 25.0%
! TCAAATAATAATTTCCTGGA+TGG + Chr6:23398016-23398035 None:intergenic 25.0%
! TGAATGAAATTCATATGCTT+TGG + Chr6:23398438-23398457 None:intergenic 25.0%
!! ACAGCTTTTAAACTATATAC+AGG - Chr6:23399040-23399059 MsG0680031618.01.T01:five_prime_UTR 25.0%
!! ATATTTTCCAGCAATAAAAG+AGG - Chr6:23398935-23398954 MsG0680031618.01.T01:CDS 25.0%
!! TATTTTCCAGCAATAAAAGA+GGG - Chr6:23398936-23398955 MsG0680031618.01.T01:CDS 25.0%
!!! CCAAATTTTAGTGACTAAAT+TGG - Chr6:23398858-23398877 MsG0680031618.01.T01:CDS 25.0%
!!! TGGATATTTTTTGTTGGTAT+TGG + Chr6:23397919-23397938 None:intergenic 25.0%
!!! TTTTGGAGGACTATATTTTA+AGG + Chr6:23397871-23397890 None:intergenic 25.0%
AAAGAAATCAGATATTTGGC+AGG - Chr6:23398994-23399013 MsG0680031618.01.T01:CDS 30.0%
AAGAACAAAGAACAATGTTG+AGG + Chr6:23397820-23397839 None:intergenic 30.0%
ACATCTCTATGATCTTTCTT+TGG + Chr6:23398059-23398078 None:intergenic 30.0%
ATGGCATGATCCAATATTAA+AGG + Chr6:23398295-23398314 None:intergenic 30.0%
GAAAATCAAAAGGTTGTTGA+AGG - Chr6:23397983-23398002 MsG0680031618.01.T01:intron 30.0%
GAGATGCTATGAAAATCAAA+AGG - Chr6:23397973-23397992 MsG0680031618.01.T01:intron 30.0%
GCCAAAAGAAATCAGATATT+TGG - Chr6:23398990-23399009 MsG0680031618.01.T01:CDS 30.0%
TCTTCTTAGGAGTTGTAAAT+GGG - Chr6:23398205-23398224 MsG0680031618.01.T01:intron 30.0%
TTCTTCTTAGGAGTTGTAAA+TGG - Chr6:23398204-23398223 MsG0680031618.01.T01:intron 30.0%
! CTTCACAATCATCTCTAAAA+TGG - Chr6:23397761-23397780 MsG0680031618.01.T01:CDS 30.0%
! GAAGCCAATTTTTATAGATC+CGG - Chr6:23398958-23398977 MsG0680031618.01.T01:CDS 30.0%
! GCCAAATATCTGATTTCTTT+TGG + Chr6:23398994-23399013 None:intergenic 30.0%
! TAAATATTGACTGAGCCAAT+AGG + Chr6:23398663-23398682 None:intergenic 30.0%
!! AGTCTCTAAAATTACCTCAA+AGG - Chr6:23398682-23398701 MsG0680031618.01.T01:CDS 30.0%
!!! ACTATATTTTAAGGCTGGTA+TGG + Chr6:23397862-23397881 None:intergenic 30.0%
AGAGCCGGATCTATAAAAAT+TGG + Chr6:23398965-23398984 None:intergenic 35.0%
ATACCATCTTGTGTAACCAA+AGG + Chr6:23398263-23398282 None:intergenic 35.0%
ATGCTATGGCAATTCTTCTT+AGG - Chr6:23398192-23398211 MsG0680031618.01.T01:intron 35.0%
ATGTTGAAGGTTGTGTTCAT+TGG + Chr6:23397894-23397913 None:intergenic 35.0%
ATTCACCATAGCAGTCATTT+GGG - Chr6:23398453-23398472 MsG0680031618.01.T01:intron 35.0%
CAAAGAACAATGTTGAGGTA+AGG + Chr6:23397815-23397834 None:intergenic 35.0%
CATGGATCAGATCTGAAAAT+TGG + Chr6:23398401-23398420 None:intergenic 35.0%
TAAGGCTGGTATGGTTAATT+TGG + Chr6:23397853-23397872 None:intergenic 35.0%
TAGTGCATCTGATTATCCTT+TGG - Chr6:23398244-23398263 MsG0680031618.01.T01:intron 35.0%
TATCCTTTGGTTACACAAGA+TGG - Chr6:23398257-23398276 MsG0680031618.01.T01:intron 35.0%
TCATCACATTAGTTCCTTTG+AGG + Chr6:23398699-23398718 None:intergenic 35.0%
TTAGGAGTTGTAAATGGGAT+TGG - Chr6:23398210-23398229 MsG0680031618.01.T01:intron 35.0%
TTCTACAACTCTTCATGCTA+TGG - Chr6:23398178-23398197 MsG0680031618.01.T01:intron 35.0%
TTGGTCAAGTAGGAAATGTT+TGG - Chr6:23398108-23398127 MsG0680031618.01.T01:intron 35.0%
TTTCCTACTTGACCAAAAAC+AGG + Chr6:23398104-23398123 None:intergenic 35.0%
TTTGGTTACACAAGATGGTA+TGG - Chr6:23398262-23398281 MsG0680031618.01.T01:intron 35.0%
! AGAGTTGTAGAAAGCATAGT+TGG + Chr6:23398170-23398189 None:intergenic 35.0%
! GTGACTAATGAACCTGTTTT+TGG - Chr6:23398089-23398108 MsG0680031618.01.T01:intron 35.0%
!! ATGCTTTGGAATTCCAGAAA+AGG + Chr6:23398424-23398443 None:intergenic 35.0%
!!! AAGGTTGTGTTCATTGGTTT+TGG + Chr6:23397888-23397907 None:intergenic 35.0%
CAATGTTGAGGTAAGGATAG+AGG + Chr6:23397808-23397827 None:intergenic 40.0%
CATTCACCATAGCAGTCATT+TGG - Chr6:23398452-23398471 MsG0680031618.01.T01:intron 40.0%
TGGTGAGTGGCCTTTAATAT+TGG - Chr6:23398282-23398301 MsG0680031618.01.T01:intron 40.0%
TTGGAATTCCAGAAAAGGCA+TGG + Chr6:23398419-23398438 None:intergenic 40.0%
TTTGGCAGGTAACTAAGCAA+AGG - Chr6:23399008-23399027 MsG0680031618.01.T01:CDS 40.0%
! GCTGGTATGGTTAATTTGGT+GGG + Chr6:23397849-23397868 None:intergenic 40.0%
! GTTGGTATTGGAGATGTTGA+AGG + Chr6:23397907-23397926 None:intergenic 40.0%
!! TTCTTTTGGCTGTAAAGAGC+CGG + Chr6:23398980-23398999 None:intergenic 40.0%
!!! GAACCTGTTTTTGGTCAAGT+AGG - Chr6:23398098-23398117 MsG0680031618.01.T01:intron 40.0%
!!! GGAGGACTATATTTTAAGGC+TGG + Chr6:23397867-23397886 None:intergenic 40.0%
!!! GTTGTGTTCATTGGTTTTGG+AGG + Chr6:23397885-23397904 None:intergenic 40.0%
ACACAAGATGGTATGGTGAG+TGG - Chr6:23398269-23398288 MsG0680031618.01.T01:intron 45.0%
ACCATAGCAGTCATTTGGGT+TGG - Chr6:23398457-23398476 MsG0680031618.01.T01:intron 45.0%
TCCAACCCAAATGACTGCTA+TGG + Chr6:23398461-23398480 None:intergenic 45.0%
! AGGGGCATAGTACAAGTGAT+TGG + Chr6:23398801-23398820 None:intergenic 45.0%
! GATCTGATCCATGCCTTTTC+TGG - Chr6:23398408-23398427 MsG0680031618.01.T01:intron 45.0%
! GGCTGGTATGGTTAATTTGG+TGG + Chr6:23397850-23397869 None:intergenic 45.0%
! TGGCTTCCCTCTTTTATTGC+TGG + Chr6:23398945-23398964 None:intergenic 45.0%
! TTGAAGGCTTTGTACCATCC+AGG - Chr6:23397999-23398018 MsG0680031618.01.T01:intron 45.0%
!!! AATTAAATTCTATAATTTTA+GGG + Chr6:23398592-23398611 None:intergenic 5.0%
!!! TAATTAAATTCTATAATTTT+AGG + Chr6:23398593-23398612 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
Chr6 gene 23397730 23399084 23397730 ID=MsG0680031618.01;Name=MsG0680031618.01
Chr6 mRNA 23397730 23399084 23397730 ID=MsG0680031618.01.T01;Parent=MsG0680031618.01;Name=MsG0680031618.01.T01;_AED=0.44;_eAED=0.44;_QI=50|1|0.66|1|1|1|3|0|237
Chr6 exon 23397730 23397868 23397730 ID=MsG0680031618.01.T01:exon:14589;Parent=MsG0680031618.01.T01
Chr6 exon 23398330 23398405 23398330 ID=MsG0680031618.01.T01:exon:14588;Parent=MsG0680031618.01.T01
Chr6 exon 23398536 23399084 23398536 ID=MsG0680031618.01.T01:exon:14587;Parent=MsG0680031618.01.T01
Chr6 five_prime_UTR 23399035 23399084 23399035 ID=MsG0680031618.01.T01:five_prime_utr;Parent=MsG0680031618.01.T01
Chr6 CDS 23398536 23399034 23398536 ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01
Chr6 CDS 23398330 23398405 23398330 ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01
Chr6 CDS 23397730 23397868 23397730 ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01
Gene Sequence

>MsG0680031618.01.T01

ATGGATATCAAAATTTTCATCATTGCCTCTATCCTTACCTCAACATTGTTCTTTGTTCTTTACATTCCCACCAAATTAACCATACCAGCCTTAAAATATAGTCCTCCAAAACCAATGAACACAACCTTCAACATCTCCAATACCAACAAAAAATATCCAATTAGTTTTGCATATTTAATTTCAGGTTCAAAAGGAGATGCTATGAAAATCAAAAGGTTGTTGAAGGCTTTGTACCATCCAGGAAATTATTATTTGATTCATATGGATCATGGTGCACCAAAGAAAGATCATAGAGATGTGATTGATTATGTGACTAATGAACCTGTTTTTGGTCAAGTAGGAAATGTTTGGATTATAAAGAAGAGTAACTTTGTTACATATAAAGGTCCAACTATGCTTTCTACAACTCTTCATGCTATGGCAATTCTTCTTAGGAGTTGTAAATGGGATTGGTTTATTAATCTTAGTGCATCTGATTATCCTTTGGTTACACAAGATGATCTGATCCATGCCTTTTCTGGAATTCCAAAGCATATGAATTTCATTCACCATAGCAGTCATTTGGGTTGGAAATTCAATAAAAGAGGGAAGCCAATTTTTATAGATCCGGCTCTTTACAGCCAAAAGAAATCAGATATTTGGCAGGTAACTAAGCAAAGGAGACTCCCAACAGCTTTTAAACTATATACAGGTGTCATTTTCTTCTGCCTTTAA

Protein sequence

>MsG0680031618.01.T01

MDIKIFIIASILTSTLFFVLYIPTKLTIPALKYSPPKPMNTTFNISNTNKKYPISFAYLISGSKGDAMKIKRLLKALYHPGNYYLIHMDHGAPKKDHRDVIDYVTNEPVFGQVGNVWIIKKSNFVTYKGPTMLSTTLHAMAILLRSCKWDWFINLSASDYPLVTQDDLIHAFSGIPKHMNFIHHSSHLGWKFNKRGKPIFIDPALYSQKKSDIWQVTKQRRLPTAFKLYTGVIFFCL*