AlfalfaGEDB | MsG0680031618.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031618.01

KEGG: -
GO: GO:0008375 GO:0016020
PFAM: PF02485

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031618.01.T01	XP_013451521.2	95.238	231	11	0	1	231	1	231	2.65E-159	457

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031618.01.T01	Q9LFQ0	55.738	183	80	1	56	237	88	270	2.05E-67	217

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000334.01	MsG0680031618.01	0.823512	1.330977e-53	1.148423e-50
MsG0180000470.01	MsG0680031618.01	0.807142	5.840063e-50	3.227821e-47
MsG0180001892.01	MsG0680031618.01	0.819202	1.314287e-52	1.004753e-49
MsG0180004389.01	MsG0680031618.01	0.804274	2.337043e-49	1.199897e-46
MsG0180004465.01	MsG0680031618.01	0.803369	3.601845e-49	1.806752e-46
MsG0180005575.01	MsG0680031618.01	0.800550	1.367345e-48	6.384783e-46
MsG0280007072.01	MsG0680031618.01	0.813099	3.038827e-51	1.966086e-48
MsG0280007432.01	MsG0680031618.01	0.814185	1.752604e-51	1.167939e-48
MsG0280007955.01	MsG0680031618.01	0.845869	3.103486e-59	5.244843e-56
MsG0280008377.01	MsG0680031618.01	0.808942	2.416415e-50	1.400024e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031618.01.T01	MTR_7g086720	68.103	232	70	2	1	231	1	229	1.03e-105	311
MsG0680031618.01.T01	MTR_6g027550	96.528	144	5	0	88	231	1	144	2.34e-102	300
MsG0680031618.01.T01	MTR_4g114140	57.692	182	76	1	57	237	76	257	1.25e-73	229
MsG0680031618.01.T01	MTR_2g085300	56.284	183	79	1	56	237	83	265	7.94e-70	220
MsG0680031618.01.T01	MTR_6g011080	47.107	242	112	5	8	237	32	269	1.05e-69	220
MsG0680031618.01.T01	MTR_0072s0020	51.500	200	96	1	39	237	72	271	5.25e-69	218
MsG0680031618.01.T01	MTR_4g022240	51.412	177	85	1	56	231	73	249	1.11e-64	207
MsG0680031618.01.T01	MTR_1g046740	50.538	186	91	1	53	237	93	278	5.09e-59	192
MsG0680031618.01.T01	MTR_0662s0030	56.250	144	62	1	95	237	15	158	1.38e-56	182
MsG0680031618.01.T01	MTR_1g108280	46.961	181	95	1	52	231	46	226	4.43e-54	179

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031618.01.T01	AT3G03690	61.429	210	77	2	22	231	24	229	2.24e-88	266
MsG0680031618.01.T01	AT4G27480	57.923	183	76	1	56	237	80	262	6.21e-73	228
MsG0680031618.01.T01	AT4G27480	57.923	183	76	1	56	237	80	262	6.21e-73	228
MsG0680031618.01.T01	AT4G27480	57.923	183	76	1	56	237	80	262	6.21e-73	228
MsG0680031618.01.T01	AT3G15350	56.284	183	79	1	56	237	81	263	3.57e-72	226
MsG0680031618.01.T01	AT3G15350	56.284	183	79	1	56	237	81	263	3.57e-72	226
MsG0680031618.01.T01	AT3G03690	59.064	171	66	2	22	192	24	190	4.23e-69	211
MsG0680031618.01.T01	AT1G53100	47.458	236	111	4	8	231	25	259	6.28e-69	218
MsG0680031618.01.T01	AT1G53100	47.458	236	111	4	8	231	25	259	7.29e-69	218
MsG0680031618.01.T01	AT5G15050	55.738	183	80	1	56	237	88	270	2.09e-68	217

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Find 48 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAATCAGATATTTGGC+AGG	0.557557	6:-23397798	MsG0680031618.01.T01:CDS
AAATTATTATTTGATTCATA+TGG	0.318689	6:-23398770	MsG0680031618.01.T01:CDS
AAGAACAAAGAACAATGTTG+AGG	0.619282	6:+23398975	None:intergenic
AAGGTTGTGTTCATTGGTTT+TGG	0.309897	6:+23398907	None:intergenic
ACATCTCTATGATCTTTCTT+TGG	0.304538	6:+23398736	None:intergenic
ACCATAGCAGTCATTTGGGT+TGG	0.447311	6:-23398335	MsG0680031618.01.T01:CDS
AGAAATTAAGTTTGGGAATA+TGG	0.178104	6:+23399052	None:intergenic
AGAGTTGTAGAAAGCATAGT+TGG	0.528565	6:+23398625	None:intergenic
AGTAACTTTGTTACATATAA+AGG	0.317512	6:-23398649	MsG0680031618.01.T01:CDS
ATACCATCTTGTGTAACCAA+AGG	0.660551	6:+23398532	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAATCAGATATTTGGC+AGG	-	Chr6:23398994-23399013	MsG0680031618.01.T01:CDS	30.0%
AAGAACAAAGAACAATGTTG+AGG	+	Chr6:23397820-23397839	None:intergenic	30.0%
ACATCTCTATGATCTTTCTT+TGG	+	Chr6:23398059-23398078	None:intergenic	30.0%
ATGGCATGATCCAATATTAA+AGG	+	Chr6:23398295-23398314	None:intergenic	30.0%
GAAAATCAAAAGGTTGTTGA+AGG	-	Chr6:23397983-23398002	MsG0680031618.01.T01:intron	30.0%
GAGATGCTATGAAAATCAAA+AGG	-	Chr6:23397973-23397992	MsG0680031618.01.T01:intron	30.0%
GCCAAAAGAAATCAGATATT+TGG	-	Chr6:23398990-23399009	MsG0680031618.01.T01:CDS	30.0%
TCTTCTTAGGAGTTGTAAAT+GGG	-	Chr6:23398205-23398224	MsG0680031618.01.T01:intron	30.0%
TTCTTCTTAGGAGTTGTAAA+TGG	-	Chr6:23398204-23398223	MsG0680031618.01.T01:intron	30.0%
AGAGCCGGATCTATAAAAAT+TGG	+	Chr6:23398965-23398984	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	23397730	23399084	23397730	ID=MsG0680031618.01;Name=MsG0680031618.01
Chr6	mRNA	23397730	23399084	23397730	ID=MsG0680031618.01.T01;Parent=MsG0680031618.01;Name=MsG0680031618.01.T01;_AED=0.44;_eAED=0.44;_QI=50\|1\|0.66\|1\|1\|1\|3\|0\|237
Chr6	exon	23397730	23397868	23397730	ID=MsG0680031618.01.T01:exon:14589;Parent=MsG0680031618.01.T01
Chr6	exon	23398330	23398405	23398330	ID=MsG0680031618.01.T01:exon:14588;Parent=MsG0680031618.01.T01
Chr6	exon	23398536	23399084	23398536	ID=MsG0680031618.01.T01:exon:14587;Parent=MsG0680031618.01.T01
Chr6	five_prime_UTR	23399035	23399084	23399035	ID=MsG0680031618.01.T01:five_prime_utr;Parent=MsG0680031618.01.T01
Chr6	CDS	23398536	23399034	23398536	ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01
Chr6	CDS	23398330	23398405	23398330	ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01
Chr6	CDS	23397730	23397868	23397730	ID=MsG0680031618.01.T01:cds;Parent=MsG0680031618.01.T01

Gene Sequence

>MsG0680031618.01.T01

ATGGATATCAAAATTTTCATCATTGCCTCTATCCTTACCTCAACATTGTTCTTTGTTCTTTACATTCCCACCAAATTAACCATACCAGCCTTAAAATATAGTCCTCCAAAACCAATGAACACAACCTTCAACATCTCCAATACCAACAAAAAATATCCAATTAGTTTTGCATATTTAATTTCAGGTTCAAAAGGAGATGCTATGAAAATCAAAAGGTTGTTGAAGGCTTTGTACCATCCAGGAAATTATTATTTGATTCATATGGATCATGGTGCACCAAAGAAAGATCATAGAGATGTGATTGATTATGTGACTAATGAACCTGTTTTTGGTCAAGTAGGAAATGTTTGGATTATAAAGAAGAGTAACTTTGTTACATATAAAGGTCCAACTATGCTTTCTACAACTCTTCATGCTATGGCAATTCTTCTTAGGAGTTGTAAATGGGATTGGTTTATTAATCTTAGTGCATCTGATTATCCTTTGGTTACACAAGATGATCTGATCCATGCCTTTTCTGGAATTCCAAAGCATATGAATTTCATTCACCATAGCAGTCATTTGGGTTGGAAATTCAATAAAAGAGGGAAGCCAATTTTTATAGATCCGGCTCTTTACAGCCAAAAGAAATCAGATATTTGGCAGGTAACTAAGCAAAGGAGACTCCCAACAGCTTTTAAACTATATACAGGTGTCATTTTCTTCTGCCTTTAA

Protein sequence

>MsG0680031618.01.T01

MDIKIFIIASILTSTLFFVLYIPTKLTIPALKYSPPKPMNTTFNISNTNKKYPISFAYLISGSKGDAMKIKRLLKALYHPGNYYLIHMDHGAPKKDHRDVIDYVTNEPVFGQVGNVWIIKKSNFVTYKGPTMLSTTLHAMAILLRSCKWDWFINLSASDYPLVTQDDLIHAFSGIPKHMNFIHHSSHLGWKFNKRGKPIFIDPALYSQKKSDIWQVTKQRRLPTAFKLYTGVIFFCL*