AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040261.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040261.01.T01 MTR_7g086720 96.512 172 6 0 1 172 228 399 6.64e-124 354
MsG0780040261.01.T01 MTR_6g027550 76.000 175 39 1 1 172 143 317 2.05e-98 287
MsG0780040261.01.T01 MTR_0072s0020 57.803 173 67 5 1 171 264 432 1.99e-64 204
MsG0780040261.01.T01 MTR_6g011080 52.632 171 81 0 1 171 262 432 1.17e-61 196
MsG0780040261.01.T01 MTR_2g085300 52.326 172 77 3 1 172 258 424 1.40e-57 186
MsG0780040261.01.T01 MTR_4g022240 48.837 172 88 0 1 172 248 419 1.49e-54 178
MsG0780040261.01.T01 MTR_0662s0030 57.692 130 55 0 1 130 151 280 1.38e-53 172
MsG0780040261.01.T01 MTR_4g114140 57.692 130 55 0 1 130 250 379 1.22e-52 172
MsG0780040261.01.T01 MTR_1g108280 43.011 186 79 3 1 172 225 397 1.33e-44 151
MsG0780040261.01.T01 MTR_1g046740 40.936 171 98 2 1 171 271 438 1.23e-37 134
MsG0780040261.01.T01 MTR_4g008010 38.953 172 103 2 2 172 237 407 3.78e-37 132
MsG0780040261.01.T01 MTR_0011s0310 34.211 114 60 2 58 171 2 100 3.28e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040261.01.T01 AT4G03340 59.538 173 64 5 1 171 256 424 3.09e-67 211
MsG0780040261.01.T01 AT4G03340 59.538 173 64 5 1 171 279 447 4.30e-67 211
MsG0780040261.01.T01 AT5G39990 54.651 172 78 0 1 172 276 447 8.54e-67 210
MsG0780040261.01.T01 AT1G03520 58.960 173 65 4 1 171 189 357 9.31e-67 207
MsG0780040261.01.T01 AT4G27480 54.651 172 73 2 1 172 255 421 3.79e-66 208
MsG0780040261.01.T01 AT4G27480 54.651 172 73 2 1 172 255 421 3.79e-66 208
MsG0780040261.01.T01 AT4G27480 54.651 172 73 2 1 172 255 421 3.79e-66 208
MsG0780040261.01.T01 AT1G03520 58.960 173 65 4 1 171 278 446 8.51e-66 207
MsG0780040261.01.T01 AT3G03690 76.106 113 27 0 1 113 228 340 1.24e-62 197
MsG0780040261.01.T01 AT5G15050 51.744 172 83 0 1 172 263 434 1.11e-61 197
MsG0780040261.01.T01 AT3G15350 55.747 174 70 4 1 172 256 424 1.29e-60 194
MsG0780040261.01.T01 AT3G15350 55.747 174 70 4 1 172 256 424 1.29e-60 194
MsG0780040261.01.T01 AT1G53100 53.529 170 73 4 3 171 258 422 5.50e-59 189
MsG0780040261.01.T01 AT1G53100 56.688 157 62 4 3 158 258 409 3.67e-57 184
MsG0780040261.01.T01 AT1G71070 42.529 174 96 2 1 172 224 395 3.12e-47 158
MsG0780040261.01.T01 AT2G37585 44.444 171 76 2 2 172 233 384 4.15e-45 152
MsG0780040261.01.T01 AT3G24040 38.953 172 103 2 1 171 244 414 1.40e-36 131

Find 30 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTAAAGATTATAGGAAAA+TGG 0.276934 7:+75050753 MsG0780040261.01.T01:CDS
TTTGTTAAACAAAATCCTTA+TGG 0.342506 7:+75050984 MsG0780040261.01.T01:CDS
AGAGACCTAGGATGTTGTTT+TGG 0.350611 7:-75050727 None:intergenic
CTGTTTACCTACACTGCATT+TGG 0.352059 7:-75051020 None:intergenic
GGTTTGAGAACTGAGAATTA+TGG 0.376756 7:+75050922 MsG0780040261.01.T01:CDS
ACTGTTTGGAAATATCCTTC+TGG 0.379198 7:-75050631 None:intergenic
CTCTAGGACTTAAAGATTAT+AGG 0.380227 7:+75050746 MsG0780040261.01.T01:CDS
ATGATCTTCACTACATTACT+TGG 0.398716 7:+75050695 MsG0780040261.01.T01:CDS
AGCCTTCTTGAGCCAGGACC+AGG 0.423280 7:-75050955 None:intergenic
TTTGGAGGGTGGTGTTCTCA+AGG 0.438791 7:+75050877 MsG0780040261.01.T01:CDS
GGTTTGCTTAAACCTGGTCC+TGG 0.447605 7:+75050943 MsG0780040261.01.T01:CDS
GTGAGTTGCATATTACTGTT+TGG 0.477123 7:-75050645 None:intergenic
CTGAGTATTGCATCGTGGGA+TGG 0.479349 7:+75050557 MsG0780040261.01.T01:CDS
TGAGTATTGCATCGTGGGAT+GGG 0.496769 7:+75050558 MsG0780040261.01.T01:CDS
AAACAACATCCTAGGTCTCT+AGG 0.497584 7:+75050730 MsG0780040261.01.T01:CDS
AATTATGGTTTGCTTAAACC+TGG 0.512607 7:+75050937 MsG0780040261.01.T01:CDS
TAGTAGAGGAGTAGAGTCCT+TGG 0.516432 7:-75050589 None:intergenic
AGACAAAGTTAGTGTAGTAG+AGG 0.518512 7:-75050603 None:intergenic
GGAAAGAACAAAGCATGCTC+AGG 0.540430 7:+75050901 MsG0780040261.01.T01:CDS
ATCCTCCAAAACAACATCCT+AGG 0.540904 7:+75050722 MsG0780040261.01.T01:CDS
CACAAAATTCAGGTTCAGCA+TGG 0.562912 7:+75050512 None:intergenic
TCTTTAAGTCCTAGAGACCT+AGG 0.565974 7:-75050739 None:intergenic
AATGTAGTGAAGATCATGGT+TGG 0.568469 7:-75050689 None:intergenic
TGATCTTCACTACATTACTT+GGG 0.576959 7:+75050696 MsG0780040261.01.T01:CDS
AAGTAATGTAGTGAAGATCA+TGG 0.594031 7:-75050693 None:intergenic
AACCTGGTCCTGGCTCAAGA+AGG 0.597108 7:+75050953 MsG0780040261.01.T01:CDS
GTGAAGATCATGGTTGGCAG+TGG 0.602943 7:-75050683 None:intergenic
AACTTTGTCTCATCACCAGA+AGG 0.613473 7:+75050616 MsG0780040261.01.T01:CDS
TGGGATGGGAAAATCTGCCA+AGG 0.635256 7:+75050572 MsG0780040261.01.T01:CDS
GGAGGGTGGTGTTCTCAAGG+TGG 0.638629 7:+75050880 MsG0780040261.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAAAGATTATAGGAAAA+TGG + Chr7:75050753-75050772 MsG0780040261.01.T01:CDS 20.0%
!! TTTGTTAAACAAAATCCTTA+TGG + Chr7:75050984-75051003 MsG0780040261.01.T01:CDS 20.0%
AAGTAATGTAGTGAAGATCA+TGG - Chr7:75050696-75050715 None:intergenic 30.0%
ATGATCTTCACTACATTACT+TGG + Chr7:75050695-75050714 MsG0780040261.01.T01:CDS 30.0%
GGCGAAAAAATTTATCCATA+AGG - Chr7:75051002-75051021 None:intergenic 30.0%
TGATCTTCACTACATTACTT+GGG + Chr7:75050696-75050715 MsG0780040261.01.T01:CDS 30.0%
!! AATTATGGTTTGCTTAAACC+TGG + Chr7:75050937-75050956 MsG0780040261.01.T01:CDS 30.0%
!! AGATCTTTTGAAGAGGTATA+AGG + Chr7:75050843-75050862 MsG0780040261.01.T01:CDS 30.0%
!! ATCTTTTGAAGAGGTATAAG+GGG + Chr7:75050845-75050864 MsG0780040261.01.T01:CDS 30.0%
!! CTCTAGGACTTAAAGATTAT+AGG + Chr7:75050746-75050765 MsG0780040261.01.T01:CDS 30.0%
!! GATCTTTTGAAGAGGTATAA+GGG + Chr7:75050844-75050863 MsG0780040261.01.T01:CDS 30.0%
!! TTGATAGAGATCTTTTGAAG+AGG + Chr7:75050836-75050855 MsG0780040261.01.T01:CDS 30.0%
AATGTAGTGAAGATCATGGT+TGG - Chr7:75050692-75050711 None:intergenic 35.0%
ACTGTTTGGAAATATCCTTC+TGG - Chr7:75050634-75050653 None:intergenic 35.0%
AGACAAAGTTAGTGTAGTAG+AGG - Chr7:75050606-75050625 None:intergenic 35.0%
GGTTTGAGAACTGAGAATTA+TGG + Chr7:75050922-75050941 MsG0780040261.01.T01:CDS 35.0%
GTGAGTTGCATATTACTGTT+TGG - Chr7:75050648-75050667 None:intergenic 35.0%
! TATAAGGGGGGATTTTCTTT+TGG + Chr7:75050859-75050878 MsG0780040261.01.T01:CDS 35.0%
!! ATTTTCTGAGTATTGCATCG+TGG + Chr7:75050552-75050571 MsG0780040261.01.T01:CDS 35.0%
!! CTCTTGAATTTTCTAGCGAA+AGG - Chr7:75050793-75050812 None:intergenic 35.0%
!! TCTTTTGAAGAGGTATAAGG+GGG + Chr7:75050846-75050865 MsG0780040261.01.T01:CDS 35.0%
!! TGAATTTTCTAGCGAAAGGA+CGG - Chr7:75050789-75050808 None:intergenic 35.0%
!! TTTTCTGAGTATTGCATCGT+GGG + Chr7:75050553-75050572 MsG0780040261.01.T01:CDS 35.0%
AAACAACATCCTAGGTCTCT+AGG + Chr7:75050730-75050749 MsG0780040261.01.T01:CDS 40.0%
AACTTTGTCTCATCACCAGA+AGG + Chr7:75050616-75050635 MsG0780040261.01.T01:CDS 40.0%
ATCCTCCAAAACAACATCCT+AGG + Chr7:75050722-75050741 MsG0780040261.01.T01:CDS 40.0%
TCTTTAAGTCCTAGAGACCT+AGG - Chr7:75050742-75050761 None:intergenic 40.0%
! TTTTTCGCCAAATGCAGTGT+AGG + Chr7:75051013-75051032 MsG0780040261.01.T01:CDS 40.0%
!! AGAGACCTAGGATGTTGTTT+TGG - Chr7:75050730-75050749 None:intergenic 40.0%
!! CTTTTGAAGAGGTATAAGGG+GGG + Chr7:75050847-75050866 MsG0780040261.01.T01:CDS 40.0%
GGAAAGAACAAAGCATGCTC+AGG + Chr7:75050901-75050920 MsG0780040261.01.T01:CDS 45.0%
TAGTAGAGGAGTAGAGTCCT+TGG - Chr7:75050592-75050611 None:intergenic 45.0%
! AAGGGGGGATTTTCTTTTGG+AGG + Chr7:75050862-75050881 MsG0780040261.01.T01:CDS 45.0%
! AGGGGGGATTTTCTTTTGGA+GGG + Chr7:75050863-75050882 MsG0780040261.01.T01:CDS 45.0%
! TGAGTATTGCATCGTGGGAT+GGG + Chr7:75050558-75050577 MsG0780040261.01.T01:CDS 45.0%
!! TTTTTGAGCCTTCTTGAGCC+AGG - Chr7:75050964-75050983 None:intergenic 45.0%
!!! GACCTAGGATGTTGTTTTGG+AGG - Chr7:75050727-75050746 None:intergenic 45.0%
GTGAAGATCATGGTTGGCAG+TGG - Chr7:75050686-75050705 None:intergenic 50.0%
TGGGATGGGAAAATCTGCCA+AGG + Chr7:75050572-75050591 MsG0780040261.01.T01:CDS 50.0%
TTTGGAGGGTGGTGTTCTCA+AGG + Chr7:75050877-75050896 MsG0780040261.01.T01:CDS 50.0%
! CTGAGTATTGCATCGTGGGA+TGG + Chr7:75050557-75050576 MsG0780040261.01.T01:CDS 50.0%
! GGTTTGCTTAAACCTGGTCC+TGG + Chr7:75050943-75050962 MsG0780040261.01.T01:CDS 50.0%
!! GGGGATTTTCTTTTGGAGGG+TGG + Chr7:75050866-75050885 MsG0780040261.01.T01:CDS 50.0%
AACCTGGTCCTGGCTCAAGA+AGG + Chr7:75050953-75050972 MsG0780040261.01.T01:CDS 55.0%
AGCCTTCTTGAGCCAGGACC+AGG - Chr7:75050958-75050977 None:intergenic 60.0%
GGAGGGTGGTGTTCTCAAGG+TGG + Chr7:75050880-75050899 MsG0780040261.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 75050520 75051038 75050520 ID=MsG0780040261.01;Name=MsG0780040261.01
Chr7 mRNA 75050520 75051038 75050520 ID=MsG0780040261.01.T01;Parent=MsG0780040261.01;Name=MsG0780040261.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|172
Chr7 exon 75050520 75051038 75050520 ID=MsG0780040261.01.T01:exon:14170;Parent=MsG0780040261.01.T01
Chr7 CDS 75050520 75051038 75050520 ID=MsG0780040261.01.T01:cds;Parent=MsG0780040261.01.T01
Gene Sequence

>MsG0780040261.01.T01

TCAGGTTCAGCATGGACAATAGTATCAAGATCATTTTCTGAGTATTGCATCGTGGGATGGGAAAATCTGCCAAGGACTCTACTCCTCTACTACACTAACTTTGTCTCATCACCAGAAGGATATTTCCAAACAGTAATATGCAACTCACAAGACTACAAGAACACCACTGCCAACCATGATCTTCACTACATTACTTGGGACAATCCTCCAAAACAACATCCTAGGTCTCTAGGACTTAAAGATTATAGGAAAATGGTTCTAAGTAGCCGTCCTTTCGCTAGAAAATTCAAGAGAAATGATATTGTTCTCGATAAAATTGATAGAGATCTTTTGAAGAGGTATAAGGGGGGATTTTCTTTTGGAGGGTGGTGTTCTCAAGGTGGAAAGAACAAAGCATGCTCAGGTTTGAGAACTGAGAATTATGGTTTGCTTAAACCTGGTCCTGGCTCAAGAAGGCTCAAAAATTTGTTAAACAAAATCCTTATGGATAAATTTTTTCGCCAAATGCAGTGTAGGTAA

Protein sequence

>MsG0780040261.01.T01

SGSAWTIVSRSFSEYCIVGWENLPRTLLLYYTNFVSSPEGYFQTVICNSQDYKNTTANHDLHYITWDNPPKQHPRSLGLKDYRKMVLSSRPFARKFKRNDIVLDKIDRDLLKRYKGGFSFGGWCSQGGKNKACSGLRTENYGLLKPGPGSRRLKNLLNKILMDKFFRQMQCR*