AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027798.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027798.01.T01 MTR_4g030210 94.932 513 25 1 1 512 1 513 0.0 971
MsG0580027798.01.T01 MTR_4g030040 81.961 510 86 2 1 509 1 505 0.0 851
MsG0580027798.01.T01 MTR_4g030040 82.341 504 88 1 7 509 3 506 0.0 849
MsG0580027798.01.T01 MTR_8g039190 72.764 492 129 2 21 508 18 508 0.0 735
MsG0580027798.01.T01 MTR_5g080050 68.713 505 147 4 14 508 8 511 0.0 688
MsG0580027798.01.T01 MTR_5g082760 69.048 504 140 3 21 508 18 521 0.0 680
MsG0580027798.01.T01 MTR_8g039070 66.057 492 126 4 21 508 18 472 0.0 638
MsG0580027798.01.T01 MTR_8g447340 46.380 511 263 4 10 509 9 519 1.31e-170 492
MsG0580027798.01.T01 MTR_2g081930 32.087 508 320 7 18 505 21 523 7.90e-78 253
MsG0580027798.01.T01 MTR_4g120720 39.465 299 144 6 82 375 2 268 2.21e-61 202
MsG0580027798.01.T01 MTR_4g081490 30.769 390 246 7 134 509 66 445 1.96e-47 171
MsG0580027798.01.T01 MTR_2g081950 31.186 388 242 7 133 500 18 400 7.79e-45 163
MsG0580027798.01.T01 MTR_2g081960 34.862 109 71 0 30 138 32 140 2.62e-19 85.1
MsG0580027798.01.T01 MTR_4g081480 32.110 109 70 2 30 134 36 144 5.57e-16 75.5
MsG0580027798.01.T01 MTR_3g116060 27.742 155 109 1 146 297 68 222 6.03e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027798.01.T01 AT1G16390 47.937 509 257 4 7 507 1 509 5.42e-178 511
MsG0580027798.01.T01 AT1G79360 46.435 519 259 7 7 509 1 516 2.03e-173 499
MsG0580027798.01.T01 AT1G79410 45.155 485 260 6 30 510 26 508 1.81e-146 430
MsG0580027798.01.T01 AT1G16370 43.114 501 278 6 16 510 15 514 7.76e-146 429
MsG0580027798.01.T01 AT1G73220 36.935 509 302 5 16 509 26 530 1.32e-109 337
MsG0580027798.01.T01 AT3G20660 31.034 493 318 10 30 510 35 517 2.64e-72 239
MsG0580027798.01.T01 AT3G51490 30.058 173 113 3 132 297 3 174 3.02e-13 72.8
MsG0580027798.01.T01 AT3G51490 30.058 173 113 3 132 297 53 224 3.49e-13 72.8
MsG0580027798.01.T01 AT3G51490 30.058 173 113 3 132 297 53 224 3.70e-13 72.8
MsG0580027798.01.T01 AT1G20840 29.560 159 101 3 146 297 68 222 3.03e-11 66.6
MsG0580027798.01.T01 AT1G20840 29.560 159 101 3 146 297 68 222 3.03e-11 66.6

Find 90 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCATGACCATGAAGATTT+TGG 0.105653 5:-68688956 None:intergenic
TCAAATTCCTTATCGGATTT+TGG 0.181510 5:+68689215 MsG0580027798.01.T01:CDS
GGAATTGGAATGGTTTATTT+TGG 0.195131 5:+68689682 MsG0580027798.01.T01:CDS
ATCTTCTCTTATGGTGTGTT+TGG 0.229402 5:+68690087 MsG0580027798.01.T01:CDS
TCTTCTCTTATGGTGTGTTT+GGG 0.236997 5:+68690088 MsG0580027798.01.T01:CDS
GAAGCAATTGCAATGCTTAA+AGG 0.243155 5:+68689499 MsG0580027798.01.T01:CDS
TTCTTCATAGGTTGCCTAAT+TGG 0.243229 5:+68689061 MsG0580027798.01.T01:CDS
TTTAGATAATAGAGATATTA+AGG 0.249467 5:+68686409 MsG0580027798.01.T01:five_prime_UTR
TAATATCTCTATTATCTAAA+TGG 0.256585 5:-68686406 None:intergenic
AGGAATTCTGTGGTTGGATA+TGG 0.258871 5:+68686429 MsG0580027798.01.T01:exon
TTGTGTCGCTACTTATTTCT+TGG 0.264486 5:+68689783 MsG0580027798.01.T01:CDS
TAAGCATTGCAATTGCTTCT+TGG 0.272991 5:-68689495 None:intergenic
TATTGTGCTTCGCAGGAAAA+AGG 0.282742 5:+68688734 MsG0580027798.01.T01:intron
TGATTCAACATGCAGATCTA+TGG 0.284386 5:+68686471 MsG0580027798.01.T01:CDS
CCACAATCTTCATTCTTCAT+AGG 0.305982 5:+68689049 MsG0580027798.01.T01:CDS
CCTTTAGCCGTTGGGAACTT+AGG 0.321956 5:+68689709 MsG0580027798.01.T01:CDS
TCGGCTTTCAAATTCCTTAT+CGG 0.326745 5:+68689208 MsG0580027798.01.T01:CDS
GAGAGGAGTTTACGAACATT+TGG 0.340782 5:+68688782 MsG0580027798.01.T01:CDS
ATGGTTCTTGGTATTGGAAT+TGG 0.344101 5:+68689667 MsG0580027798.01.T01:CDS
GCAAAATCCCAAAATCTTCA+TGG 0.345391 5:+68688948 MsG0580027798.01.T01:CDS
TGTGTTATGTGTGTTGTTGT+TGG 0.359401 5:+68689862 MsG0580027798.01.T01:CDS
ACAAGCTCTTTGTGATACTA+TGG 0.364002 5:+68690164 MsG0580027798.01.T01:CDS
TTTAATGTAATATTTGTACT+AGG 0.364732 5:+68690217 MsG0580027798.01.T01:exon
GCTATAATTTACTCAATCAT+TGG 0.374272 5:+68689421 MsG0580027798.01.T01:CDS
CTTAGGGTTCGACATTTACT+TGG 0.376808 5:+68689726 MsG0580027798.01.T01:CDS
ATGTGTGTTGTTGTTGGATC+AGG 0.394930 5:+68689868 MsG0580027798.01.T01:CDS
CTTTAGCCGTTGGGAACTTA+GGG 0.397423 5:+68689710 MsG0580027798.01.T01:CDS
GCAATGATGGTTCTTGGTAT+TGG 0.397888 5:+68689661 MsG0580027798.01.T01:CDS
GTCGTGTTCCTCACACTTGT+CGG 0.398211 5:-68689985 None:intergenic
GTTGAAGCATTACTTTGTCT+TGG 0.404742 5:+68690284 MsG0580027798.01.T01:three_prime_UTR
GAAAAGAATGGTTGCAATGA+TGG 0.411878 5:+68689648 MsG0580027798.01.T01:CDS
TTTATGCATTTAGGTTCGTT+TGG 0.414086 5:+68690248 MsG0580027798.01.T01:three_prime_UTR
CTACTTATTTCTTGGAGAAT+CGG 0.419849 5:+68689791 MsG0580027798.01.T01:CDS
ATGGTTGCAATGATGGTTCT+TGG 0.423914 5:+68689655 MsG0580027798.01.T01:CDS
ATTGAAAATGCAAGAATTGA+CGG 0.431608 5:-68689823 None:intergenic
GGAGTACAAGGAATCAAAGT+TGG 0.436137 5:+68689889 MsG0580027798.01.T01:CDS
TGTTGTGTTTAATGCCTTGA+TGG 0.436862 5:+68689750 MsG0580027798.01.T01:CDS
ATAACATACATCGTTAGCTT+TGG 0.436938 5:-68686500 None:intergenic
TCCCAAAATCTTCATGGTCA+TGG 0.441703 5:+68688954 MsG0580027798.01.T01:CDS
CTAATGCAAAAGAACCAATT+AGG 0.442316 5:-68689075 None:intergenic
CCTAAGTTCCCAACGGCTAA+AGG 0.454852 5:-68689709 None:intergenic
CAAAGTTGTTTGAGACAAGC+TGG 0.456719 5:+68689620 MsG0580027798.01.T01:CDS
TTGGTATGCCTTTAGCCGTT+GGG 0.459450 5:+68689701 MsG0580027798.01.T01:CDS
AAGAATTGCAAATGATCAAT+CGG 0.461038 5:+68690388 MsG0580027798.01.T01:three_prime_UTR
CTTGCTTTGCCAGAGACTAA+AGG 0.464667 5:+68690141 MsG0580027798.01.T01:CDS
AGAGCTTGTCCTTTAGTCTC+TGG 0.466490 5:-68690150 None:intergenic
GTTGGATATGGCTTCTATTG+AGG 0.479176 5:+68686441 MsG0580027798.01.T01:exon
CCCAAAATCTTCATGGTCAT+GGG 0.481349 5:+68688955 MsG0580027798.01.T01:CDS
ACTTTAGCTGATACATCCCT+TGG 0.481619 5:+68689100 MsG0580027798.01.T01:CDS
ATGATTGAGTAAATTATAGC+TGG 0.481999 5:-68689418 None:intergenic
GAGGAACACGACAACGTCAT+TGG 0.501684 5:+68689996 MsG0580027798.01.T01:CDS
TTTGGTATGCCTTTAGCCGT+TGG 0.503043 5:+68689700 MsG0580027798.01.T01:CDS
TTAATGTAATATTTGTACTA+GGG 0.515395 5:+68690218 MsG0580027798.01.T01:exon
TAAAAGCCACCTTGGAGACT+CGG 0.517844 5:-68689458 None:intergenic
TCTATCATTTCATCTGATGA+AGG 0.520096 5:-68688761 None:intergenic
TCTTGGTATTGGAATTGGAA+TGG 0.520997 5:+68689672 MsG0580027798.01.T01:CDS
GGAGTTTACGAACATTTGGA+TGG 0.525287 5:+68688786 MsG0580027798.01.T01:CDS
GTTGTTGGATCAGGAGTACA+AGG 0.526318 5:+68689877 MsG0580027798.01.T01:CDS
TAGTACGGAATGGAGGTTCA+CGG 0.527397 5:+68689285 MsG0580027798.01.T01:CDS
ATATTAAGGAATTCTGTGGT+TGG 0.528597 5:+68686423 MsG0580027798.01.T01:five_prime_UTR
ATGAACGCCAAAATCCGATA+AGG 0.531545 5:-68689222 None:intergenic
CCTTGCATTAAAAGCCACCT+TGG 0.537931 5:-68689466 None:intergenic
AAGCTGGGCTTTGAAAAGAA+TGG 0.539389 5:+68689636 MsG0580027798.01.T01:CDS
GAGTACAAGGAATCAAAGTT+GGG 0.541950 5:+68689890 MsG0580027798.01.T01:CDS
AACCAATGTTTGGATTTACT+CGG 0.542219 5:+68689189 MsG0580027798.01.T01:CDS
CTTCTCTTATGGTGTGTTTG+GGG 0.557389 5:+68690089 MsG0580027798.01.T01:CDS
TGTGCAAGTAGCTTCGTCAC+AGG 0.558448 5:+68689022 MsG0580027798.01.T01:CDS
AATCTGTGCAATGCCATGTT+GGG 0.563621 5:-68688895 None:intergenic
AAAGTTGTTTGAGACAAGCT+GGG 0.565151 5:+68689621 MsG0580027798.01.T01:CDS
ACAAGGAATCAAAGTTGGGT+TGG 0.576373 5:+68689894 MsG0580027798.01.T01:CDS
GAATCTGTGCAATGCCATGT+TGG 0.577316 5:-68688896 None:intergenic
TGAAAACACAATGATGATTG+AGG 0.578552 5:-68689167 None:intergenic
AAATCTTCATGGTCATGGGA+TGG 0.583642 5:+68688959 MsG0580027798.01.T01:CDS
ACAAGACGGTATTTCCATCA+AGG 0.595551 5:-68689764 None:intergenic
TGCATTTAGCAACAAACTCG+CGG 0.597000 5:-68689551 None:intergenic
TCTTTGTTACCGAGTCTCCA+AGG 0.601885 5:+68689449 MsG0580027798.01.T01:CDS
AGAGATATTAAGGAATTCTG+TGG 0.604430 5:+68686419 MsG0580027798.01.T01:five_prime_UTR
ACGGAATGGAGGTTCACGGT+TGG 0.606795 5:+68689289 MsG0580027798.01.T01:CDS
AGCCGAGTAAATCCAAACAT+TGG 0.608114 5:-68689191 None:intergenic
CATCAGATGAAATGATAGAG+AGG 0.609645 5:+68688765 MsG0580027798.01.T01:CDS
GAGTTTACGAACATTTGGAT+GGG 0.612517 5:+68688787 MsG0580027798.01.T01:CDS
AATGCTTAAAGGAATGAGCT+CGG 0.634915 5:+68689510 MsG0580027798.01.T01:CDS
AAATAAGTAGCGACACAAGA+CGG 0.636296 5:-68689778 None:intergenic
AGTTGTTTCCGACAAGTGTG+AGG 0.640373 5:+68689977 MsG0580027798.01.T01:CDS
ACACCGACGATTACCCAACA+TGG 0.652539 5:+68688882 MsG0580027798.01.T01:CDS
GTCGAACCCTAAGTTCCCAA+CGG 0.657526 5:-68689716 None:intergenic
TTAGGGTACATGTCATTACC+CGG 0.657645 5:+68689337 MsG0580027798.01.T01:CDS
CCTATGAAGAATGAAGATTG+TGG 0.667133 5:-68689049 None:intergenic
ATGCCATGTTGGGTAATCGT+CGG 0.699036 5:-68688885 None:intergenic
TTGTTACCGAGTCTCCAAGG+TGG 0.709073 5:+68689452 MsG0580027798.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAAAAACCAAAA+AGG + Chr5:68688348-68688367 MsG0580027798.01.T01:intron 10.0%
!!! AGAAATTTTTATTTTTTTAG+GGG + Chr5:68687470-68687489 MsG0580027798.01.T01:intron 10.0%
!!! ATCTCAAATAAAAATTATTT+TGG - Chr5:68687634-68687653 None:intergenic 10.0%
!!! ATGATATATAATTTTATGTA+TGG + Chr5:68687602-68687621 MsG0580027798.01.T01:intron 10.0%
!!! GTTTTTCTTTTTTTAATTAT+TGG + Chr5:68688113-68688132 MsG0580027798.01.T01:intron 10.0%
!!! TTTTATTTTTTTTATTCTTG+TGG + Chr5:68687811-68687830 MsG0580027798.01.T01:intron 10.0%
!! CAAATATAGAAAAAACTTAT+CGG - Chr5:68686944-68686963 None:intergenic 15.0%
!! CTATCATTATGAAATAAAAT+AGG - Chr5:68687227-68687246 None:intergenic 15.0%
!! GTGAAAATAATGAAATATAT+AGG + Chr5:68687399-68687418 MsG0580027798.01.T01:intron 15.0%
!! TAATATCTCTATTATCTAAA+TGG - Chr5:68686409-68686428 None:intergenic 15.0%
!! TTAATGTAATATTTGTACTA+GGG + Chr5:68690218-68690237 MsG0580027798.01.T01:exon 15.0%
!! TTTAATGTAATATTTGTACT+AGG + Chr5:68690217-68690236 MsG0580027798.01.T01:exon 15.0%
!! TTTAGATAATAGAGATATTA+AGG + Chr5:68686409-68686428 MsG0580027798.01.T01:five_prime_UTR 15.0%
!! TTTGATAAAACTTTATAAAG+AGG - Chr5:68687095-68687114 None:intergenic 15.0%
!!! ATAGTATGTTTTACTTTTAA+CGG + Chr5:68687868-68687887 MsG0580027798.01.T01:intron 15.0%
!!! ATAGTTTTGCATTTTATATA+TGG - Chr5:68686582-68686601 None:intergenic 15.0%
!!! CTATTTTATTTCATAATGAT+AGG + Chr5:68687225-68687244 MsG0580027798.01.T01:intron 15.0%
!!! CTTTATTTTACATGAATTAT+TGG + Chr5:68688137-68688156 MsG0580027798.01.T01:intron 15.0%
!!! GAGTAAAAAAATGAATATTT+TGG + Chr5:68687443-68687462 MsG0580027798.01.T01:intron 15.0%
!!! GCATATATTTTATCATTTAA+AGG + Chr5:68688282-68688301 MsG0580027798.01.T01:intron 15.0%
!!! GTAGAAGTTTTTTTTTTTTT+TGG + Chr5:68687348-68687367 MsG0580027798.01.T01:intron 15.0%
!!! TTTTCATGTTGAAAAATAAA+AGG + Chr5:68687747-68687766 MsG0580027798.01.T01:intron 15.0%
!! AAAAAACAATCATATCTCAA+TGG + Chr5:68688990-68689009 MsG0580027798.01.T01:CDS 20.0%
!! AAAAATGATATCTTCTCTTA+TGG + Chr5:68690078-68690097 MsG0580027798.01.T01:CDS 20.0%
!! ACAAAAGATTATTGTTAAGT+TGG - Chr5:68688227-68688246 None:intergenic 20.0%
!! ACAACTACAATTAATGAATA+TGG - Chr5:68688092-68688111 None:intergenic 20.0%
!! ACAATTAATGAATATGGATA+GGG - Chr5:68688086-68688105 None:intergenic 20.0%
!! TACAATTAATGAATATGGAT+AGG - Chr5:68688087-68688106 None:intergenic 20.0%
!! TGAAATATATAGGTACTATA+TGG + Chr5:68687409-68687428 MsG0580027798.01.T01:intron 20.0%
!! TGTTGGAACAAAATAAAATA+TGG - Chr5:68687989-68688008 None:intergenic 20.0%
!! TTGGTCATTTATATTAGTAA+AGG - Chr5:68688320-68688339 None:intergenic 20.0%
!!! AATATTTTTGCTTCACATTA+GGG + Chr5:68689320-68689339 MsG0580027798.01.T01:CDS 20.0%
!!! AATTTTTATTTTTTTAGGGG+AGG + Chr5:68687473-68687492 MsG0580027798.01.T01:intron 20.0%
!!! GAAAAATAAAAGGAGTATTT+TGG + Chr5:68687757-68687776 MsG0580027798.01.T01:intron 20.0%
!!! TAGACAATATTTCTTCTTTT+AGG - Chr5:68686865-68686884 None:intergenic 20.0%
!!! TTTTATTTCATAATGATAGG+TGG + Chr5:68687228-68687247 MsG0580027798.01.T01:intron 20.0%
! AAGAATTGCAAATGATCAAT+CGG + Chr5:68690388-68690407 MsG0580027798.01.T01:three_prime_UTR 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - Chr5:68688431-68688450 None:intergenic 25.0%
! ATGAGAATACATAACTGTAT+TGG + Chr5:68687713-68687732 MsG0580027798.01.T01:intron 25.0%
! ATGATTGAGTAAATTATAGC+TGG - Chr5:68689421-68689440 None:intergenic 25.0%
! ATTGAAAATGCAAGAATTGA+CGG - Chr5:68689826-68689845 None:intergenic 25.0%
! GCTATAATTTACTCAATCAT+TGG + Chr5:68689421-68689440 MsG0580027798.01.T01:CDS 25.0%
! TAATAAGAACATAACATGAG+AGG - Chr5:68686710-68686729 None:intergenic 25.0%
! TATTCTTGTGGTTTAGATAT+TGG + Chr5:68687823-68687842 MsG0580027798.01.T01:intron 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + Chr5:68688443-68688462 MsG0580027798.01.T01:intron 25.0%
!! AGGTACTATATGGATTATTT+AGG + Chr5:68687419-68687438 MsG0580027798.01.T01:intron 25.0%
!! CTCATTAGATTAATCCTTTT+TGG - Chr5:68688365-68688384 None:intergenic 25.0%
!!! ATGTGTTCTTGTTTTGTTAA+CGG + Chr5:68689255-68689274 MsG0580027798.01.T01:CDS 25.0%
!!! CTTAAAATCTTGTTTTGCTT+TGG + Chr5:68686653-68686672 MsG0580027798.01.T01:intron 25.0%
!!! GAATATTTTTGCTTCACATT+AGG + Chr5:68689319-68689338 MsG0580027798.01.T01:CDS 25.0%
!!! GAGGTGCAATAATATTTTTA+TGG + Chr5:68687492-68687511 MsG0580027798.01.T01:intron 25.0%
!!! GATATGATTGTTTTTTGTGA+TGG - Chr5:68688986-68689005 None:intergenic 25.0%
!!! GTTTTTTGTTTTTGCACAAA+AGG - Chr5:68687195-68687214 None:intergenic 25.0%
!!! GTTTTTTGTTTTTGCAGAAA+AGG - Chr5:68687286-68687305 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + Chr5:68688430-68688449 MsG0580027798.01.T01:intron 25.0%
AAACAATGGAGAGAGAAAAA+AGG + Chr5:68688457-68688476 MsG0580027798.01.T01:intron 30.0%
AACCAATGTTTGGATTTACT+CGG + Chr5:68689189-68689208 MsG0580027798.01.T01:CDS 30.0%
AACGTGGATCAAAAAACATA+GGG + Chr5:68686801-68686820 MsG0580027798.01.T01:intron 30.0%
AGAGATATTAAGGAATTCTG+TGG + Chr5:68686419-68686438 MsG0580027798.01.T01:five_prime_UTR 30.0%
AGGAATTCACAAAGTATGAA+AGG - Chr5:68686687-68686706 None:intergenic 30.0%
ATATTAAGGAATTCTGTGGT+TGG + Chr5:68686423-68686442 MsG0580027798.01.T01:five_prime_UTR 30.0%
ATTTATACTCTTGTGGTGTA+TGG + Chr5:68688008-68688027 MsG0580027798.01.T01:intron 30.0%
CGTGGAAAAATGTTTACATT+TGG + Chr5:68689386-68689405 MsG0580027798.01.T01:CDS 30.0%
CTAATGCAAAAGAACCAATT+AGG - Chr5:68689078-68689097 None:intergenic 30.0%
GTCAAAAAGTTGTCAAAAAG+TGG + Chr5:68688524-68688543 MsG0580027798.01.T01:intron 30.0%
GTTAACGGAAAAAGTTAGTA+CGG + Chr5:68689270-68689289 MsG0580027798.01.T01:CDS 30.0%
TCAACAAAGTTGTCAACAAA+TGG - Chr5:68688405-68688424 None:intergenic 30.0%
TCACAAAGTATGAAAGGAAA+GGG - Chr5:68686681-68686700 None:intergenic 30.0%
TCTATCATTTCATCTGATGA+AGG - Chr5:68688764-68688783 None:intergenic 30.0%
TGAAAACACAATGATGATTG+AGG - Chr5:68689170-68689189 None:intergenic 30.0%
TTCACAAAGTATGAAAGGAA+AGG - Chr5:68686682-68686701 None:intergenic 30.0%
TTCCAACATTTATACTCTTG+TGG + Chr5:68688001-68688020 MsG0580027798.01.T01:intron 30.0%
! AAATTTTTATTGTGCTTCGC+AGG + Chr5:68688727-68688746 MsG0580027798.01.T01:intron 30.0%
! ATAACATACATCGTTAGCTT+TGG - Chr5:68686503-68686522 None:intergenic 30.0%
! CATTATCAACGTTCTTTTGT+TGG - Chr5:68687943-68687962 None:intergenic 30.0%
! CTACTTATTTCTTGGAGAAT+CGG + Chr5:68689791-68689810 MsG0580027798.01.T01:CDS 30.0%
! GGAATTGGAATGGTTTATTT+TGG + Chr5:68689682-68689701 MsG0580027798.01.T01:CDS 30.0%
! GTTTTTGCACAAAAGGATAT+AGG - Chr5:68687188-68687207 None:intergenic 30.0%
! GTTTTTGCAGAAAAGGATAT+AGG - Chr5:68687279-68687298 None:intergenic 30.0%
! TCAAATTCCTTATCGGATTT+TGG + Chr5:68689215-68689234 MsG0580027798.01.T01:CDS 30.0%
! TTGATCGAGAACTTTTTGTA+TGG - Chr5:68686778-68686797 None:intergenic 30.0%
! TTGTGTTTTCAACCAATGTT+TGG + Chr5:68689179-68689198 MsG0580027798.01.T01:CDS 30.0%
! TTTATGCATTTAGGTTCGTT+TGG + Chr5:68690248-68690267 MsG0580027798.01.T01:three_prime_UTR 30.0%
!!! AGTCCTTTTTTAATATCTGC+TGG + Chr5:68690051-68690070 MsG0580027798.01.T01:CDS 30.0%
!!! ATCGTTTTTTAGTGCTACTA+CGG + Chr5:68689924-68689943 MsG0580027798.01.T01:CDS 30.0%
!!! CAACATGTTTTTTCTTCCAA+GGG - Chr5:68689119-68689138 None:intergenic 30.0%
!!! CATTTTGAGAGCATTCAATT+TGG + Chr5:68688196-68688215 MsG0580027798.01.T01:intron 30.0%
!!! GGTCTGTTTTTTATGCATTT+AGG + Chr5:68690239-68690258 MsG0580027798.01.T01:three_prime_UTR 30.0%
!!! GTCCTTTTTTAATATCTGCT+GGG + Chr5:68690052-68690071 MsG0580027798.01.T01:CDS 30.0%
!!! TCAACATGTTTTTTCTTCCA+AGG - Chr5:68689120-68689139 None:intergenic 30.0%
!!! TTCGTTTGGTTTGTTGTTTT+TGG + Chr5:68690262-68690281 MsG0580027798.01.T01:three_prime_UTR 30.0%
!!! TTTTGGGATTTTGCAAATGT+TGG - Chr5:68688942-68688961 None:intergenic 30.0%
AAAAAGTTAGTACGGAATGG+AGG + Chr5:68689278-68689297 MsG0580027798.01.T01:CDS 35.0%
AAAAGTTGCATGTTGTCTTG+TGG + Chr5:68687783-68687802 MsG0580027798.01.T01:intron 35.0%
AAATAAGTAGCGACACAAGA+CGG - Chr5:68689781-68689800 None:intergenic 35.0%
AATGCTTAAAGGAATGAGCT+CGG + Chr5:68689510-68689529 MsG0580027798.01.T01:CDS 35.0%
ACAAGCTCTTTGTGATACTA+TGG + Chr5:68690164-68690183 MsG0580027798.01.T01:CDS 35.0%
AGCTTGTCATATGCATTATG+AGG - Chr5:68688600-68688619 None:intergenic 35.0%
ATCTTCTCTTATGGTGTGTT+TGG + Chr5:68690087-68690106 MsG0580027798.01.T01:CDS 35.0%
CAACGTGGATCAAAAAACAT+AGG + Chr5:68686800-68686819 MsG0580027798.01.T01:intron 35.0%
CATCAGATGAAATGATAGAG+AGG + Chr5:68688765-68688784 MsG0580027798.01.T01:CDS 35.0%
CCACAATCTTCATTCTTCAT+AGG + Chr5:68689049-68689068 MsG0580027798.01.T01:CDS 35.0%
CCTATGAAGAATGAAGATTG+TGG - Chr5:68689052-68689071 None:intergenic 35.0%
CTCCCAGCAGATATTAAAAA+AGG - Chr5:68690057-68690076 None:intergenic 35.0%
GAAAAGAATGGTTGCAATGA+TGG + Chr5:68689648-68689667 MsG0580027798.01.T01:CDS 35.0%
GAAGCAATTGCAATGCTTAA+AGG + Chr5:68689499-68689518 MsG0580027798.01.T01:CDS 35.0%
GAGTACAAGGAATCAAAGTT+GGG + Chr5:68689890-68689909 MsG0580027798.01.T01:CDS 35.0%
GAGTTTACGAACATTTGGAT+GGG + Chr5:68688787-68688806 MsG0580027798.01.T01:CDS 35.0%
GCAAAATCCCAAAATCTTCA+TGG + Chr5:68688948-68688967 MsG0580027798.01.T01:CDS 35.0%
TAAGAACATAACATGAGAGG+AGG - Chr5:68686707-68686726 None:intergenic 35.0%
TCGAAAAACATAAGCTACAC+CGG - Chr5:68689359-68689378 None:intergenic 35.0%
TCGGCTTTCAAATTCCTTAT+CGG + Chr5:68689208-68689227 MsG0580027798.01.T01:CDS 35.0%
TCTTCTCTTATGGTGTGTTT+GGG + Chr5:68690088-68690107 MsG0580027798.01.T01:CDS 35.0%
TGATTCAACATGCAGATCTA+TGG + Chr5:68686471-68686490 MsG0580027798.01.T01:CDS 35.0%
TGTTGTGTTTAATGCCTTGA+TGG + Chr5:68689750-68689769 MsG0580027798.01.T01:CDS 35.0%
TTCTTCATAGGTTGCCTAAT+TGG + Chr5:68689061-68689080 MsG0580027798.01.T01:CDS 35.0%
TTTCTTAACATGTGCCCTAA+GGG - Chr5:68686842-68686861 None:intergenic 35.0%
! AAAGTTGTTTGAGACAAGCT+GGG + Chr5:68689621-68689640 MsG0580027798.01.T01:CDS 35.0%
! ATCAACGTTCTTTTGTTGGA+AGG - Chr5:68687939-68687958 None:intergenic 35.0%
! ATGGTTCTTGGTATTGGAAT+TGG + Chr5:68689667-68689686 MsG0580027798.01.T01:CDS 35.0%
! CACCACAAGAGTATAAATGT+TGG - Chr5:68688006-68688025 None:intergenic 35.0%
! GTGAGACAAGCTATTGTTTT+CGG + Chr5:68690018-68690037 MsG0580027798.01.T01:CDS 35.0%
! GTTGAAGCATTACTTTGTCT+TGG + Chr5:68690284-68690303 MsG0580027798.01.T01:three_prime_UTR 35.0%
! TAAGCATTGCAATTGCTTCT+TGG - Chr5:68689498-68689517 None:intergenic 35.0%
! TCTTGGTATTGGAATTGGAA+TGG + Chr5:68689672-68689691 MsG0580027798.01.T01:CDS 35.0%
! TGTGTTATGTGTGTTGTTGT+TGG + Chr5:68689862-68689881 MsG0580027798.01.T01:CDS 35.0%
! TTGTGTCGCTACTTATTTCT+TGG + Chr5:68689783-68689802 MsG0580027798.01.T01:CDS 35.0%
!! ATACACACTCTAAAGTTGCA+TGG - Chr5:68688646-68688665 None:intergenic 35.0%
!! ATGTTTTTCGATCTTCATCG+TGG + Chr5:68689368-68689387 MsG0580027798.01.T01:CDS 35.0%
!! ATTGTTAACTTGTGCCCTTA+GGG + Chr5:68686825-68686844 MsG0580027798.01.T01:intron 35.0%
!! GGGAAATGTTAACTTGTTGA+GGG - Chr5:68686982-68687001 None:intergenic 35.0%
!! TACACACTCTAAAGTTGCAT+GGG - Chr5:68688645-68688664 None:intergenic 35.0%
!! TATTGTTAACTTGTGCCCTT+AGG + Chr5:68686824-68686843 MsG0580027798.01.T01:intron 35.0%
!! TGGGAAATGTTAACTTGTTG+AGG - Chr5:68686983-68687002 None:intergenic 35.0%
!!! CTATGTTTTTTGATCCACGT+TGG - Chr5:68686802-68686821 None:intergenic 35.0%
!!! CTTTTTTAATATCTGCTGGG+AGG + Chr5:68690055-68690074 MsG0580027798.01.T01:CDS 35.0%
AAATCTTCATGGTCATGGGA+TGG + Chr5:68688959-68688978 MsG0580027798.01.T01:CDS 40.0%
AAATGCACCCCCTAAACAAA+AGG + Chr5:68689567-68689586 MsG0580027798.01.T01:CDS 40.0%
AATCTGTGCAATGCCATGTT+GGG - Chr5:68688898-68688917 None:intergenic 40.0%
ACAAGACGGTATTTCCATCA+AGG - Chr5:68689767-68689786 None:intergenic 40.0%
ACAAGGAATCAAAGTTGGGT+TGG + Chr5:68689894-68689913 MsG0580027798.01.T01:CDS 40.0%
ACTTTAGCTGATACATCCCT+TGG + Chr5:68689100-68689119 MsG0580027798.01.T01:CDS 40.0%
AGCCGAGTAAATCCAAACAT+TGG - Chr5:68689194-68689213 None:intergenic 40.0%
AGGAATTCTGTGGTTGGATA+TGG + Chr5:68686429-68686448 MsG0580027798.01.T01:exon 40.0%
ATGAACGCCAAAATCCGATA+AGG - Chr5:68689225-68689244 None:intergenic 40.0%
ATGGTTGCAATGATGGTTCT+TGG + Chr5:68689655-68689674 MsG0580027798.01.T01:CDS 40.0%
CCCAAAATCTTCATGGTCAT+GGG + Chr5:68688955-68688974 MsG0580027798.01.T01:CDS 40.0%
CGAAAAACATAAGCTACACC+GGG - Chr5:68689358-68689377 None:intergenic 40.0%
CGGAAAAAGTTAGTACGGAA+TGG + Chr5:68689275-68689294 MsG0580027798.01.T01:CDS 40.0%
CTTAGGGTTCGACATTTACT+TGG + Chr5:68689726-68689745 MsG0580027798.01.T01:CDS 40.0%
CTTCTCTTATGGTGTGTTTG+GGG + Chr5:68690089-68690108 MsG0580027798.01.T01:CDS 40.0%
GAGAGGAGTTTACGAACATT+TGG + Chr5:68688782-68688801 MsG0580027798.01.T01:CDS 40.0%
GCAATGATGGTTCTTGGTAT+TGG + Chr5:68689661-68689680 MsG0580027798.01.T01:CDS 40.0%
GGAGTACAAGGAATCAAAGT+TGG + Chr5:68689889-68689908 MsG0580027798.01.T01:CDS 40.0%
GGAGTTTACGAACATTTGGA+TGG + Chr5:68688786-68688805 MsG0580027798.01.T01:CDS 40.0%
GGTAGCAAAGAGAGAAAAAG+TGG + Chr5:68686373-68686392 MsG0580027798.01.T01:five_prime_UTR 40.0%
GTTGGATATGGCTTCTATTG+AGG + Chr5:68686441-68686460 MsG0580027798.01.T01:exon 40.0%
GTTTCTTAACATGTGCCCTA+AGG - Chr5:68686843-68686862 None:intergenic 40.0%
TATTGTGCTTCGCAGGAAAA+AGG + Chr5:68688734-68688753 MsG0580027798.01.T01:intron 40.0%
TCCCAAAATCTTCATGGTCA+TGG + Chr5:68688954-68688973 MsG0580027798.01.T01:CDS 40.0%
TCTTGTGGTGTATGGAGAAT+TGG + Chr5:68688016-68688035 MsG0580027798.01.T01:intron 40.0%
TGCATTTAGCAACAAACTCG+CGG - Chr5:68689554-68689573 None:intergenic 40.0%
TTAGGGTACATGTCATTACC+CGG + Chr5:68689337-68689356 MsG0580027798.01.T01:CDS 40.0%
! ATGTGTGTTGTTGTTGGATC+AGG + Chr5:68689868-68689887 MsG0580027798.01.T01:CDS 40.0%
! CAAAGTTGTTTGAGACAAGC+TGG + Chr5:68689620-68689639 MsG0580027798.01.T01:CDS 40.0%
! CCCATGACCATGAAGATTTT+GGG - Chr5:68688958-68688977 None:intergenic 40.0%
! CTTGCATTTTCAATAGCGAG+TGG + Chr5:68689832-68689851 MsG0580027798.01.T01:CDS 40.0%
! TCCCATGACCATGAAGATTT+TGG - Chr5:68688959-68688978 None:intergenic 40.0%
!! AAACCCGAAGCCTTTTGTTT+AGG - Chr5:68689580-68689599 None:intergenic 40.0%
!! AACCCGAAGCCTTTTGTTTA+GGG - Chr5:68689579-68689598 None:intergenic 40.0%
!! AAGCTGGGCTTTGAAAAGAA+TGG + Chr5:68689636-68689655 MsG0580027798.01.T01:CDS 40.0%
!! GGATTTTGGCGTTCATCTAT+AGG + Chr5:68689229-68689248 MsG0580027798.01.T01:CDS 40.0%
ACCCCCTAAACAAAAGGCTT+CGG + Chr5:68689573-68689592 MsG0580027798.01.T01:CDS 45.0%
AGAGCTTGTCCTTTAGTCTC+TGG - Chr5:68690153-68690172 None:intergenic 45.0%
AGTTCTCGATCAATCCAACG+TGG + Chr5:68686785-68686804 MsG0580027798.01.T01:intron 45.0%
ATGCCATGTTGGGTAATCGT+CGG - Chr5:68688888-68688907 None:intergenic 45.0%
CCTTGCATTAAAAGCCACCT+TGG - Chr5:68689469-68689488 None:intergenic 45.0%
CTTGCTTTGCCAGAGACTAA+AGG + Chr5:68690141-68690160 MsG0580027798.01.T01:CDS 45.0%
CTTTAGCCGTTGGGAACTTA+GGG + Chr5:68689710-68689729 MsG0580027798.01.T01:CDS 45.0%
GAATCTGTGCAATGCCATGT+TGG - Chr5:68688899-68688918 None:intergenic 45.0%
TAAAAGCCACCTTGGAGACT+CGG - Chr5:68689461-68689480 None:intergenic 45.0%
TAGTACGGAATGGAGGTTCA+CGG + Chr5:68689285-68689304 MsG0580027798.01.T01:CDS 45.0%
TCTTTGTTACCGAGTCTCCA+AGG + Chr5:68689449-68689468 MsG0580027798.01.T01:CDS 45.0%
TTGGTATGCCTTTAGCCGTT+GGG + Chr5:68689701-68689720 MsG0580027798.01.T01:CDS 45.0%
TTTGGTATGCCTTTAGCCGT+TGG + Chr5:68689700-68689719 MsG0580027798.01.T01:CDS 45.0%
! AGTTGTTTCCGACAAGTGTG+AGG + Chr5:68689977-68689996 MsG0580027798.01.T01:CDS 45.0%
! CCAAGGTGGCTTTTAATGCA+AGG + Chr5:68689466-68689485 MsG0580027798.01.T01:CDS 45.0%
! GTTGTTGGATCAGGAGTACA+AGG + Chr5:68689877-68689896 MsG0580027798.01.T01:CDS 45.0%
!! ACCCGAAGCCTTTTGTTTAG+GGG - Chr5:68689578-68689597 None:intergenic 45.0%
!!! AAGAAATTTTTATTTTTTTA+GGG + Chr5:68687469-68687488 MsG0580027798.01.T01:intron 5.0%
!!! TAAGAAATTTTTATTTTTTT+AGG + Chr5:68687468-68687487 MsG0580027798.01.T01:intron 5.0%
!!! TTAATATATAATATCTTTTT+GGG - Chr5:68687002-68687021 None:intergenic 5.0%
!!! TTTAATATATAATATCTTTT+TGG - Chr5:68687003-68687022 None:intergenic 5.0%
!!! TTTTTTTTTTAGTTTAAAAT+TGG - Chr5:68688339-68688358 None:intergenic 5.0%
ACACCGACGATTACCCAACA+TGG + Chr5:68688882-68688901 MsG0580027798.01.T01:CDS 50.0%
CCCCCTAAACAAAAGGCTTC+GGG + Chr5:68689574-68689593 MsG0580027798.01.T01:CDS 50.0%
CCTAAGTTCCCAACGGCTAA+AGG - Chr5:68689712-68689731 None:intergenic 50.0%
CCTTTAGCCGTTGGGAACTT+AGG + Chr5:68689709-68689728 MsG0580027798.01.T01:CDS 50.0%
GAGGAACACGACAACGTCAT+TGG + Chr5:68689996-68690015 MsG0580027798.01.T01:CDS 50.0%
GTCGAACCCTAAGTTCCCAA+CGG - Chr5:68689719-68689738 None:intergenic 50.0%
GTCGTGTTCCTCACACTTGT+CGG - Chr5:68689988-68690007 None:intergenic 50.0%
TGTGCAAGTAGCTTCGTCAC+AGG + Chr5:68689022-68689041 MsG0580027798.01.T01:CDS 50.0%
TTGTTACCGAGTCTCCAAGG+TGG + Chr5:68689452-68689471 MsG0580027798.01.T01:CDS 50.0%
!! CCCGAAGCCTTTTGTTTAGG+GGG - Chr5:68689577-68689596 None:intergenic 50.0%
ACGGAATGGAGGTTCACGGT+TGG + Chr5:68689289-68689308 MsG0580027798.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 68686371 68690421 68686371 ID=MsG0580027798.01;Name=MsG0580027798.01
Chr5 mRNA 68686371 68690421 68686371 ID=MsG0580027798.01.T01;Parent=MsG0580027798.01;Name=MsG0580027798.01.T01;_AED=0.28;_eAED=0.28;_QI=77|1|1|1|1|1|2|200|512
Chr5 exon 68686371 68686513 68686371 ID=MsG0580027798.01.T01:exon:14378;Parent=MsG0580027798.01.T01
Chr5 exon 68688749 68690421 68688749 ID=MsG0580027798.01.T01:exon:14379;Parent=MsG0580027798.01.T01
Chr5 five_prime_UTR 68686371 68686447 68686371 ID=MsG0580027798.01.T01:five_prime_utr;Parent=MsG0580027798.01.T01
Chr5 CDS 68686448 68686513 68686448 ID=MsG0580027798.01.T01:cds;Parent=MsG0580027798.01.T01
Chr5 CDS 68688749 68690221 68688749 ID=MsG0580027798.01.T01:cds;Parent=MsG0580027798.01.T01
Chr5 three_prime_UTR 68690222 68690421 68690222 ID=MsG0580027798.01.T01:three_prime_utr;Parent=MsG0580027798.01.T01
Gene Sequence

>MsG0580027798.01.T01

ATGGCTTCTATTGAGGTGCTTTCTGATTCAACATGCAGATCTATGGATGATACCAAAGCTAACGATGAAAAAGGTATTCCTTCATCAGATGAAATGATAGAGAGGAGTTTACGAACATTTGGATGGGTAGAATTTGTGCAATGCATTCTTGTATCATTCGCAATGTTCTTCGACGCACAACAATCATTCATAGCAATTTACACCGACGATTACCCAACATGGCATTGCACAGATTCAACAACATGCAATTCATATTCCAACATTTGCAAAATCCCAAAATCTTCATGGTCATGGGATGGACCATCACAAAAAACAATCATATCTCAATGGAATCTTGAATGTGCAAGTAGCTTCGTCACAGGTTTACCACAATCTTCATTCTTCATAGGTTGCCTAATTGGTTCTTTTGCATTAGCTACTTTAGCTGATACATCCCTTGGAAGAAAAAACATGTTGATTATCTCTTGTTTATCAATGTCAATAACCTCAATCATCATTGTGTTTTCAACCAATGTTTGGATTTACTCGGCTTTCAAATTCCTTATCGGATTTTGGCGTTCATCTATAGGTACATGTGTTCTTGTTTTGTTAACGGAAAAAGTTAGTACGGAATGGAGGTTCACGGTTGGAATTGTTGAATATTTTTGCTTCACATTAGGGTACATGTCATTACCCGGTGTAGCTTATGTTTTTCGATCTTCATCGTGGAAAAATGTTTACATTTGGACATCAATTCCAGCTATAATTTACTCAATCATTGGTTATTTCTTTGTTACCGAGTCTCCAAGGTGGCTTTTAATGCAAGGTAGAAACCAAGAAGCAATTGCAATGCTTAAAGGAATGAGCTCGGAAGAAAATGCTGAATTAACCGCGAGTTTGTTGCTAAATGCACCCCCTAAACAAAAGGCTTCGGGTTTTCAACTTTACTCATCAATAGCAAAGTTGTTTGAGACAAGCTGGGCTTTGAAAAGAATGGTTGCAATGATGGTTCTTGGTATTGGAATTGGAATGGTTTATTTTGGTATGCCTTTAGCCGTTGGGAACTTAGGGTTCGACATTTACTTGGCTGTTGTGTTTAATGCCTTGATGGAAATACCGTCTTGTGTCGCTACTTATTTCTTGGAGAATCGGCGAAGAAAGCCGTCAATTCTTGCATTTTCAATAGCGAGTGGTGTATGTTGTGTTATGTGTGTTGTTGTTGGATCAGGAGTACAAGGAATCAAAGTTGGGTTGGCGCTGACATCGTTTTTTAGTGCTACTACGGCTTATAATGTGTTTCTCATTTACATTATAGAGTTGTTTCCGACAAGTGTGAGGAACACGACAACGTCATTGGTGAGACAAGCTATTGTTTTCGGCAACATTTTTAGTCCTTTTTTAATATCTGCTGGGAGGAAAAATGATATCTTCTCTTATGGTGTGTTTGGGGTTGTTATTATGTTGTCATGTGTTACATTGCTTGCTTTGCCAGAGACTAAAGGACAAGCTCTTTGTGATACTATGGATCAACAAGAGAAAAAAGACAAAAGTTATGTTTAA
Protein sequence

>MsG0580027798.01.T01

MASIEVLSDSTCRSMDDTKANDEKGIPSSDEMIERSLRTFGWVEFVQCILVSFAMFFDAQQSFIAIYTDDYPTWHCTDSTTCNSYSNICKIPKSSWSWDGPSQKTIISQWNLECASSFVTGLPQSSFFIGCLIGSFALATLADTSLGRKNMLIISCLSMSITSIIIVFSTNVWIYSAFKFLIGFWRSSIGTCVLVLLTEKVSTEWRFTVGIVEYFCFTLGYMSLPGVAYVFRSSSWKNVYIWTSIPAIIYSIIGYFFVTESPRWLLMQGRNQEAIAMLKGMSSEENAELTASLLLNAPPKQKASGFQLYSSIAKLFETSWALKRMVAMMVLGIGIGMVYFGMPLAVGNLGFDIYLAVVFNALMEIPSCVATYFLENRRRKPSILAFSIASGVCCVMCVVVGSGVQGIKVGLALTSFFSATTAYNVFLIYIIELFPTSVRNTTTSLVRQAIVFGNIFSPFLISAGRKNDIFSYGVFGVVIMLSCVTLLALPETKGQALCDTMDQQEKKDKSYV*