AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046457.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046457.01.T01 MTR_3g039810 70.270 74 15 3 1 71 1 70 1.57e-27 95.5
MsG0880046457.01.T01 MTR_6g012220 69.565 69 17 2 1 69 1 65 1.17e-26 93.2
MsG0880046457.01.T01 MTR_3g100650 62.264 53 17 1 22 71 78 130 1.49e-16 71.6
MsG0880046457.01.T01 MTR_8g467340 57.627 59 20 1 17 70 57 115 4.10e-16 71.2
MsG0880046457.01.T01 MTR_1g077020 60.000 55 17 2 21 71 6 59 7.21e-16 68.6
MsG0880046457.01.T01 MTR_5g065440 60.377 53 18 1 22 71 56 108 4.19e-15 67.4
MsG0880046457.01.T01 MTR_7g010300 55.556 54 19 1 22 70 86 139 4.68e-15 68.2
MsG0880046457.01.T01 MTR_2g076780 50.000 66 27 2 8 70 24 86 7.75e-15 66.6
MsG0880046457.01.T01 MTR_4g134580 59.649 57 18 2 24 76 113 168 3.97e-14 65.5
MsG0880046457.01.T01 MTR_8g069080 56.000 50 19 1 24 70 69 118 7.88e-14 64.3
MsG0880046457.01.T01 MTR_5g020510 52.459 61 24 2 17 73 92 151 1.11e-13 63.9
MsG0880046457.01.T01 MTR_5g032600 58.000 50 18 1 24 70 78 127 1.78e-13 63.2
MsG0880046457.01.T01 MTR_6g012280 59.615 52 16 2 24 70 91 142 2.04e-13 63.5
MsG0880046457.01.T01 MTR_6g073013 56.000 50 18 1 24 69 19 68 2.23e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046457.01.T01 AT1G18835 64.384 73 24 1 1 71 1 73 4.91e-26 91.7
MsG0880046457.01.T01 AT3G28917 55.952 84 24 2 1 71 1 84 5.13e-23 84.3
MsG0880046457.01.T01 AT1G74660 69.811 53 13 1 22 71 37 89 4.27e-19 74.7
MsG0880046457.01.T01 AT1G74660 69.811 53 13 1 22 71 37 89 6.12e-19 73.9
MsG0880046457.01.T01 AT3G50890 52.778 72 27 3 6 71 40 110 1.98e-16 70.9
MsG0880046457.01.T01 AT1G14440 59.649 57 18 2 19 71 85 140 6.96e-16 70.1
MsG0880046457.01.T01 AT1G14440 59.649 57 18 2 19 71 85 140 6.96e-16 70.1
MsG0880046457.01.T01 AT2G18350 60.784 51 17 1 24 71 82 132 1.22e-15 68.9
MsG0880046457.01.T01 AT2G02540 61.111 54 16 2 21 70 84 136 1.71e-15 68.9
MsG0880046457.01.T01 AT2G02540 61.111 54 16 2 21 70 84 136 1.71e-15 68.9
MsG0880046457.01.T01 AT5G15210 56.364 55 19 1 21 70 53 107 2.64e-15 68.2
MsG0880046457.01.T01 AT5G42780 60.000 50 19 1 24 72 64 113 6.57e-15 66.6
MsG0880046457.01.T01 AT4G24660 61.111 54 16 2 22 71 47 99 7.50e-15 66.2
MsG0880046457.01.T01 AT5G39760 50.000 66 28 1 9 69 41 106 9.89e-15 67.0
MsG0880046457.01.T01 AT4G24660 61.111 54 16 2 22 71 47 99 1.11e-14 65.9
MsG0880046457.01.T01 AT1G69600 58.491 53 17 1 22 69 29 81 2.36e-14 65.5
MsG0880046457.01.T01 AT3G28920 55.932 59 21 1 22 75 50 108 3.30e-14 65.5
MsG0880046457.01.T01 AT5G60480 51.852 54 21 1 21 69 34 87 8.65e-14 63.5
MsG0880046457.01.T01 AT5G65410 56.863 51 19 1 24 71 75 125 1.10e-13 63.9
MsG0880046457.01.T01 AT1G75240 52.542 59 25 1 16 71 68 126 1.89e-13 63.5
MsG0880046457.01.T01 AT1G14687 52.174 46 20 1 24 69 7 50 8.90e-11 54.7

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAAATATTGTTGGATA+TGG 0.243499 8:+73611481 MsG0880046457.01.T01:CDS
ATGGAATTCATGGCATGTTC+AGG 0.335777 8:+73611559 MsG0880046457.01.T01:CDS
TCATCTTCAAGAAATATTGT+TGG 0.392599 8:+73611475 MsG0880046457.01.T01:CDS
GTGCTGCTTGCGGTTGTCAT+AGG 0.419461 8:+73611599 MsG0880046457.01.T01:CDS
GTCATAGGAACTTCCACAAA+AGG 0.480871 8:+73611614 MsG0880046457.01.T01:CDS
CTATGCTGTTGATGGATGTA+TGG 0.485307 8:+73611540 MsG0880046457.01.T01:CDS
AAGAATCATGCAGCATATAG+TGG 0.485623 8:+73611514 MsG0880046457.01.T01:CDS
AGTGGAGGCTATGCTGTTGA+TGG 0.489516 8:+73611532 MsG0880046457.01.T01:CDS
TGATGGATGTATGGAATTCA+TGG 0.495255 8:+73611549 MsG0880046457.01.T01:CDS
TGGAATTCATGGCATGTTCA+GGG 0.512543 8:+73611560 MsG0880046457.01.T01:CDS
AAAACGGCAAGTGGTGGTGG+TGG 0.605250 8:+73611435 MsG0880046457.01.T01:CDS
GCACTTACATGTGCTGCTTG+CGG 0.620281 8:+73611589 MsG0880046457.01.T01:CDS
GGAATTCATGGCATGTTCAG+GGG 0.647724 8:+73611561 MsG0880046457.01.T01:CDS
AATCATGCAGCATATAGTGG+AGG 0.667408 8:+73611517 MsG0880046457.01.T01:CDS
GTATTGCAAAGTACAGACTG+AGG 0.757439 8:+73611640 MsG0880046457.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAGAAATATTGTTGGATA+TGG + Chr8:73611481-73611500 MsG0880046457.01.T01:CDS 25.0%
! TCATCTTCAAGAAATATTGT+TGG + Chr8:73611475-73611494 MsG0880046457.01.T01:CDS 25.0%
! TTTGCAATACTTTCCTTTTG+TGG - Chr8:73611630-73611649 None:intergenic 30.0%
AAGAATCATGCAGCATATAG+TGG + Chr8:73611514-73611533 MsG0880046457.01.T01:CDS 35.0%
! TGATGGATGTATGGAATTCA+TGG + Chr8:73611549-73611568 MsG0880046457.01.T01:CDS 35.0%
AATCATGCAGCATATAGTGG+AGG + Chr8:73611517-73611536 MsG0880046457.01.T01:CDS 40.0%
ATGGAATTCATGGCATGTTC+AGG + Chr8:73611559-73611578 MsG0880046457.01.T01:CDS 40.0%
GTCATAGGAACTTCCACAAA+AGG + Chr8:73611614-73611633 MsG0880046457.01.T01:CDS 40.0%
TGGAATTCATGGCATGTTCA+GGG + Chr8:73611560-73611579 MsG0880046457.01.T01:CDS 40.0%
! CTATGCTGTTGATGGATGTA+TGG + Chr8:73611540-73611559 MsG0880046457.01.T01:CDS 40.0%
GGAATTCATGGCATGTTCAG+GGG + Chr8:73611561-73611580 MsG0880046457.01.T01:CDS 45.0%
AGTGGAGGCTATGCTGTTGA+TGG + Chr8:73611532-73611551 MsG0880046457.01.T01:CDS 50.0%
GAAAAAACGGCAAGTGGTGG+TGG + Chr8:73611432-73611451 MsG0880046457.01.T01:CDS 50.0%
!! GCACTTACATGTGCTGCTTG+CGG + Chr8:73611589-73611608 MsG0880046457.01.T01:CDS 50.0%
!! AAAACGGCAAGTGGTGGTGG+TGG + Chr8:73611435-73611454 MsG0880046457.01.T01:CDS 55.0%
!! GTGCTGCTTGCGGTTGTCAT+AGG + Chr8:73611599-73611618 MsG0880046457.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 73611430 73611660 73611430 ID=MsG0880046457.01;Name=MsG0880046457.01
Chr8 mRNA 73611430 73611660 73611430 ID=MsG0880046457.01.T01;Parent=MsG0880046457.01;Name=MsG0880046457.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|76
Chr8 exon 73611430 73611660 73611430 ID=MsG0880046457.01.T01:exon:4053;Parent=MsG0880046457.01.T01
Chr8 CDS 73611430 73611660 73611430 ID=MsG0880046457.01.T01:cds;Parent=MsG0880046457.01.T01
Gene Sequence

>MsG0880046457.01.T01

ATGAAAAAACGGCAAGTGGTGGTGGTGGTGAAACGCAACAGTACTTCATCTTCAAGAAATATTGTTGGATATGGAGAGTGTCAAAAGAATCATGCAGCATATAGTGGAGGCTATGCTGTTGATGGATGTATGGAATTCATGGCATGTTCAGGGGAAGAAGCACTTACATGTGCTGCTTGCGGTTGTCATAGGAACTTCCACAAAAGGAAAGTATTGCAAAGTACAGACTGA

Protein sequence

>MsG0880046457.01.T01

MKKRQVVVVVKRNSTSSSRNIVGYGECQKNHAAYSGGYAVDGCMEFMACSGEEALTCAACGCHRNFHKRKVLQSTD*