AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038744.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038744.01.T01 MTR_7g061550 99.444 180 1 0 6 185 180 359 1.88e-128 365
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038744.01.T01 AT2G37630 63.187 182 53 6 6 176 185 363 5.97e-62 196

Find 58 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTCAATTTCTGCTCTT+TGG 0.188451 7:+52183352 None:intergenic
GACAACGCGCCTCTTGTTCT+TGG 0.307535 7:-52183680 MsG0780038744.01.T01:CDS
AGTGTCACTGACGGTGAATT+TGG 0.344648 7:+52183764 None:intergenic
TAGAATTGAAGGAGAATATA+GGG 0.345153 7:-52183408 MsG0780038744.01.T01:CDS
ATAGAATTGAAGGAGAATAT+AGG 0.366625 7:-52183409 MsG0780038744.01.T01:CDS
GACGGTGAATTTGGCCTAAC+AGG 0.373066 7:+52183773 None:intergenic
ACAACGCGCCTCTTGTTCTT+GGG 0.374191 7:-52183679 MsG0780038744.01.T01:CDS
GGAGGAACAAGCAGTTGCTT+TGG 0.413662 7:-52183432 MsG0780038744.01.T01:CDS
CTGCTACTGTCGAGGGAGAT+AGG 0.417667 7:+52183738 None:intergenic
TCAACAATGCCGCACCTGTT+AGG 0.423262 7:-52183787 MsG0780038744.01.T01:CDS
GAATATAGGGAACAGTTAGC+AGG 0.428199 7:-52183395 MsG0780038744.01.T01:CDS
GTTGCTTTGGATAGAATTGA+AGG 0.460120 7:-52183419 MsG0780038744.01.T01:CDS
TCAAGGCATGCTGAATCAAA+TGG 0.464258 7:-52183272 MsG0780038744.01.T01:CDS
AGAACAAGAGGCGCGTTGTC+CGG 0.468651 7:+52183683 None:intergenic
CTTGTTCTAGAAACTTAGTA+AGG 0.469265 7:+52183306 None:intergenic
GCCTCCTCCGTGGATGCAAC+CGG 0.471595 7:-52183714 MsG0780038744.01.T01:CDS
GCAGAAATTGACTGAACAAT+GGG 0.483539 7:-52183345 MsG0780038744.01.T01:CDS
AGCGAAGGTAAAAGCTTTGA+GGG 0.495317 7:-52183453 MsG0780038744.01.T01:CDS
CCTCGTACCGGTTGCATCCA+CGG 0.501659 7:+52183707 None:intergenic
ACTAAGTTTCTAGAACAAGT+AGG 0.508354 7:-52183302 MsG0780038744.01.T01:CDS
CTAAGTTTCTAGAACAAGTA+GGG 0.513298 7:-52183301 MsG0780038744.01.T01:CDS
AACAAGTAGGGTGTAGATCA+AGG 0.517136 7:-52183289 MsG0780038744.01.T01:CDS
ACCGGTTGCATCCACGGAGG+AGG 0.517761 7:+52183713 None:intergenic
CATGGCGCTGTTCTAAGTTA+CGG 0.519277 7:-52183644 MsG0780038744.01.T01:CDS
AAGCGAAGGTAAAAGCTTTG+AGG 0.521296 7:-52183454 MsG0780038744.01.T01:CDS
GATGCAACCGGTACGAGGAC+CGG 0.528845 7:-52183702 MsG0780038744.01.T01:CDS
AAGTCGAAGGAGGGAAAAGA+TGG 0.533991 7:-52183486 MsG0780038744.01.T01:CDS
CACATAAGAAAGAGGCGGCA+TGG 0.534804 7:-52183550 MsG0780038744.01.T01:CDS
GCGTTGTCCGGTCCTCGTAC+CGG 0.539158 7:+52183695 None:intergenic
CAGAGAAAGCAAGTCGAAGG+AGG 0.553564 7:-52183496 MsG0780038744.01.T01:CDS
GGAAGAAGTGCATCATGCAT+TGG 0.554304 7:-52183576 MsG0780038744.01.T01:CDS
GGTTGATTGCTGCAAGGAGT+TGG 0.563650 7:-52183597 MsG0780038744.01.T01:CDS
AGCAGAAATTGACTGAACAA+TGG 0.573036 7:-52183346 MsG0780038744.01.T01:CDS
CAGTAGCAGCGCCTCCTCCG+TGG 0.575710 7:-52183724 MsG0780038744.01.T01:CDS
AGGAGGCGCTGCTACTGTCG+AGG 0.582880 7:+52183730 None:intergenic
AAGCCGAGTGGAGCTGCAGT+TGG 0.595479 7:-52183522 MsG0780038744.01.T01:CDS
CTTGGGAACGTAGCTCCACA+TGG 0.599562 7:-52183662 MsG0780038744.01.T01:CDS
GATGGAGGAAATCGAAGCGA+AGG 0.603003 7:-52183468 MsG0780038744.01.T01:CDS
TCTAAGTTACGGCGAAAGCA+TGG 0.605377 7:-52183633 MsG0780038744.01.T01:CDS
ACTCCAACTGCAGCTCCACT+CGG 0.608842 7:+52183519 None:intergenic
AGAGAAAGCAAGTCGAAGGA+GGG 0.612523 7:-52183495 MsG0780038744.01.T01:CDS
AGTCAGAGAAAGCAAGTCGA+AGG 0.613297 7:-52183499 MsG0780038744.01.T01:CDS
AAGCATGGTAATGTCTGAGT+TGG 0.613423 7:-52183618 MsG0780038744.01.T01:CDS
ATTGGCAGCACATAAGAAAG+AGG 0.615820 7:-52183558 MsG0780038744.01.T01:CDS
AATATAGGGAACAGTTAGCA+GGG 0.620687 7:-52183394 MsG0780038744.01.T01:CDS
TGAGTTGGTTGATTGCTGCA+AGG 0.626354 7:-52183603 MsG0780038744.01.T01:CDS
CCGTGGATGCAACCGGTACG+AGG 0.628739 7:-52183707 MsG0780038744.01.T01:CDS
GGAGGCGCTGCTACTGTCGA+GGG 0.631075 7:+52183731 None:intergenic
TGAATTTGGCCTAACAGGTG+CGG 0.641843 7:+52183778 None:intergenic
GCTACGTTCCCAAGAACAAG+AGG 0.644153 7:+52183671 None:intergenic
GGCATGGAGGTTAAGCCGAG+TGG 0.659534 7:-52183534 MsG0780038744.01.T01:CDS
TCGAAGGAGGGAAAAGATGG+AGG 0.666714 7:-52183483 MsG0780038744.01.T01:CDS
TAACTTAGAACAGCGCCATG+TGG 0.674992 7:+52183647 None:intergenic
GGCAGCACATAAGAAAGAGG+CGG 0.680405 7:-52183555 MsG0780038744.01.T01:CDS
GATAGGCTAAGTGTCACTGA+CGG 0.714066 7:+52183755 None:intergenic
GAAGGTAAAAGCTTTGAGGG+AGG 0.724090 7:-52183450 MsG0780038744.01.T01:CDS
ATAAGAAAGAGGCGGCATGG+AGG 0.734242 7:-52183547 MsG0780038744.01.T01:CDS
CGTACCGGTTGCATCCACGG+AGG 0.799948 7:+52183710 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAGAATTGAAGGAGAATAT+AGG - Chr7:52183656-52183675 MsG0780038744.01.T01:CDS 25.0%
! TAGAATTGAAGGAGAATATA+GGG - Chr7:52183657-52183676 MsG0780038744.01.T01:CDS 25.0%
!! ACTAAGTTTCTAGAACAAGT+AGG - Chr7:52183763-52183782 MsG0780038744.01.T01:CDS 30.0%
!! CTAAGTTTCTAGAACAAGTA+GGG - Chr7:52183764-52183783 MsG0780038744.01.T01:CDS 30.0%
!! CTTGTTCTAGAAACTTAGTA+AGG + Chr7:52183762-52183781 None:intergenic 30.0%
AATATAGGGAACAGTTAGCA+GGG - Chr7:52183671-52183690 MsG0780038744.01.T01:CDS 35.0%
AGCAGAAATTGACTGAACAA+TGG - Chr7:52183719-52183738 MsG0780038744.01.T01:CDS 35.0%
GCAGAAATTGACTGAACAAT+GGG - Chr7:52183720-52183739 MsG0780038744.01.T01:CDS 35.0%
TTCAGTCAATTTCTGCTCTT+TGG + Chr7:52183716-52183735 None:intergenic 35.0%
! GTTGCTTTGGATAGAATTGA+AGG - Chr7:52183646-52183665 MsG0780038744.01.T01:CDS 35.0%
AACAAGTAGGGTGTAGATCA+AGG - Chr7:52183776-52183795 MsG0780038744.01.T01:CDS 40.0%
AAGCGAAGGTAAAAGCTTTG+AGG - Chr7:52183611-52183630 MsG0780038744.01.T01:CDS 40.0%
ATTGGCAGCACATAAGAAAG+AGG - Chr7:52183507-52183526 MsG0780038744.01.T01:CDS 40.0%
GAATATAGGGAACAGTTAGC+AGG - Chr7:52183670-52183689 MsG0780038744.01.T01:CDS 40.0%
TCAAGGCATGCTGAATCAAA+TGG - Chr7:52183793-52183812 MsG0780038744.01.T01:CDS 40.0%
! AAGCATGGTAATGTCTGAGT+TGG - Chr7:52183447-52183466 MsG0780038744.01.T01:CDS 40.0%
! AGCGAAGGTAAAAGCTTTGA+GGG - Chr7:52183612-52183631 MsG0780038744.01.T01:CDS 40.0%
AAGTCGAAGGAGGGAAAAGA+TGG - Chr7:52183579-52183598 MsG0780038744.01.T01:CDS 45.0%
AGAGAAAGCAAGTCGAAGGA+GGG - Chr7:52183570-52183589 MsG0780038744.01.T01:CDS 45.0%
AGTCAGAGAAAGCAAGTCGA+AGG - Chr7:52183566-52183585 MsG0780038744.01.T01:CDS 45.0%
TAACTTAGAACAGCGCCATG+TGG + Chr7:52183421-52183440 None:intergenic 45.0%
TCTAAGTTACGGCGAAAGCA+TGG - Chr7:52183432-52183451 MsG0780038744.01.T01:CDS 45.0%
TGAATTTGGCCTAACAGGTG+CGG + Chr7:52183290-52183309 None:intergenic 45.0%
! AGTGTCACTGACGGTGAATT+TGG + Chr7:52183304-52183323 None:intergenic 45.0%
! CATGGCGCTGTTCTAAGTTA+CGG - Chr7:52183421-52183440 MsG0780038744.01.T01:CDS 45.0%
! GATAGGCTAAGTGTCACTGA+CGG + Chr7:52183313-52183332 None:intergenic 45.0%
! GGAAGAAGTGCATCATGCAT+TGG - Chr7:52183489-52183508 MsG0780038744.01.T01:CDS 45.0%
!! GAAGGTAAAAGCTTTGAGGG+AGG - Chr7:52183615-52183634 MsG0780038744.01.T01:CDS 45.0%
!! TGAGTTGGTTGATTGCTGCA+AGG - Chr7:52183462-52183481 MsG0780038744.01.T01:CDS 45.0%
ACAACGCGCCTCTTGTTCTT+GGG - Chr7:52183386-52183405 MsG0780038744.01.T01:CDS 50.0%
ATAAGAAAGAGGCGGCATGG+AGG - Chr7:52183518-52183537 MsG0780038744.01.T01:CDS 50.0%
CACATAAGAAAGAGGCGGCA+TGG - Chr7:52183515-52183534 MsG0780038744.01.T01:CDS 50.0%
CAGAGAAAGCAAGTCGAAGG+AGG - Chr7:52183569-52183588 MsG0780038744.01.T01:CDS 50.0%
GACGGTGAATTTGGCCTAAC+AGG + Chr7:52183295-52183314 None:intergenic 50.0%
GATGGAGGAAATCGAAGCGA+AGG - Chr7:52183597-52183616 MsG0780038744.01.T01:CDS 50.0%
GCTACGTTCCCAAGAACAAG+AGG + Chr7:52183397-52183416 None:intergenic 50.0%
GGAGGAACAAGCAGTTGCTT+TGG - Chr7:52183633-52183652 MsG0780038744.01.T01:CDS 50.0%
GGCAGCACATAAGAAAGAGG+CGG - Chr7:52183510-52183529 MsG0780038744.01.T01:CDS 50.0%
GGTTGATTGCTGCAAGGAGT+TGG - Chr7:52183468-52183487 MsG0780038744.01.T01:CDS 50.0%
TCAACAATGCCGCACCTGTT+AGG - Chr7:52183278-52183297 MsG0780038744.01.T01:CDS 50.0%
TCGAAGGAGGGAAAAGATGG+AGG - Chr7:52183582-52183601 MsG0780038744.01.T01:CDS 50.0%
ACTCCAACTGCAGCTCCACT+CGG + Chr7:52183549-52183568 None:intergenic 55.0%
CTGCTACTGTCGAGGGAGAT+AGG + Chr7:52183330-52183349 None:intergenic 55.0%
CTTGGGAACGTAGCTCCACA+TGG - Chr7:52183403-52183422 MsG0780038744.01.T01:CDS 55.0%
GACAACGCGCCTCTTGTTCT+TGG - Chr7:52183385-52183404 MsG0780038744.01.T01:CDS 55.0%
! AGAACAAGAGGCGCGTTGTC+CGG + Chr7:52183385-52183404 None:intergenic 55.0%
AAGCCGAGTGGAGCTGCAGT+TGG - Chr7:52183543-52183562 MsG0780038744.01.T01:CDS 60.0%
GATGCAACCGGTACGAGGAC+CGG - Chr7:52183363-52183382 MsG0780038744.01.T01:CDS 60.0%
GGCATGGAGGTTAAGCCGAG+TGG - Chr7:52183531-52183550 MsG0780038744.01.T01:CDS 60.0%
!! CCTCGTACCGGTTGCATCCA+CGG + Chr7:52183361-52183380 None:intergenic 60.0%
ACCGGTTGCATCCACGGAGG+AGG + Chr7:52183355-52183374 None:intergenic 65.0%
CCGTGGATGCAACCGGTACG+AGG - Chr7:52183358-52183377 MsG0780038744.01.T01:CDS 65.0%
GCCTCCTCCGTGGATGCAAC+CGG - Chr7:52183351-52183370 MsG0780038744.01.T01:CDS 65.0%
GCGTTGTCCGGTCCTCGTAC+CGG + Chr7:52183373-52183392 None:intergenic 65.0%
!! AGGAGGCGCTGCTACTGTCG+AGG + Chr7:52183338-52183357 None:intergenic 65.0%
!! CGTACCGGTTGCATCCACGG+AGG + Chr7:52183358-52183377 None:intergenic 65.0%
!! GGAGGCGCTGCTACTGTCGA+GGG + Chr7:52183337-52183356 None:intergenic 65.0%
CAGTAGCAGCGCCTCCTCCG+TGG - Chr7:52183341-52183360 MsG0780038744.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 52183265 52183822 52183265 ID=MsG0780038744.01;Name=MsG0780038744.01
Chr7 mRNA 52183265 52183822 52183265 ID=MsG0780038744.01.T01;Parent=MsG0780038744.01;Name=MsG0780038744.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|185
Chr7 exon 52183265 52183822 52183265 ID=MsG0780038744.01.T01:exon:9511;Parent=MsG0780038744.01.T01
Chr7 CDS 52183265 52183822 52183265 ID=MsG0780038744.01.T01:cds;Parent=MsG0780038744.01.T01
Gene Sequence

>MsG0780038744.01.T01

ATGGCTTTCAAATTCAACAATGCCGCACCTGTTAGGCCAAATTCACCGTCAGTGACACTTAGCCTATCTCCCTCGACAGTAGCAGCGCCTCCTCCGTGGATGCAACCGGTACGAGGACCGGACAACGCGCCTCTTGTTCTTGGGAACGTAGCTCCACATGGCGCTGTTCTAAGTTACGGCGAAAGCATGGTAATGTCTGAGTTGGTTGATTGCTGCAAGGAGTTGGAAGAAGTGCATCATGCATTGGCAGCACATAAGAAAGAGGCGGCATGGAGGTTAAGCCGAGTGGAGCTGCAGTTGGAGTCAGAGAAAGCAAGTCGAAGGAGGGAAAAGATGGAGGAAATCGAAGCGAAGGTAAAAGCTTTGAGGGAGGAACAAGCAGTTGCTTTGGATAGAATTGAAGGAGAATATAGGGAACAGTTAGCAGGGCTGAGAAGAGATGCTGAAACCAAAGAGCAGAAATTGACTGAACAATGGGCTGCGAAGCATTTGCGCCTTACTAAGTTTCTAGAACAAGTAGGGTGTAGATCAAGGCATGCTGAATCAAATGGAAGATAG

Protein sequence

>MsG0780038744.01.T01

MAFKFNNAAPVRPNSPSVTLSLSPSTVAAPPPWMQPVRGPDNAPLVLGNVAPHGAVLSYGESMVMSELVDCCKELEEVHHALAAHKKEAAWRLSRVELQLESEKASRRREKMEEIEAKVKALREEQAVALDRIEGEYREQLAGLRRDAETKEQKLTEQWAAKHLRLTKFLEQVGCRSRHAESNGR*